Hygienic indicator bacteria in the 2 species of wild-caught Japanese cicadas, large brown cicadas and robust cicadas
Takuro Ueda; Bontetsu Haga; Hodaka Suzuki, ラスト(シニア)オーサー, Entomophagy is considered a potent dietary practice to replace animal protein due to its highly nutritious and environmentally sustainable features. While entomophagy has many advantages, safety and security concerns still remain, especially for wild-caught insects, not farm-reared insects. This study aimed to clarify the potential microbiological hazards of eating wild-caught cicadas. Large brown cicadas and robust cicadas, which were commonly inhabited in Japan, were caught and examined the hygienic indicator bacteria, standard plate counts (SPC) and Enterobacteriaceae. Then, representative colonies of Enterobacteriaceae were identified. Also, the distribution of bacteria, i.e. the surface or inside the body of the cicadas, and the changes in the bacterial number during the storage of the dead cicadas were examined. SPC was in the range of 3.0 to 9.0 log CFU/g and Enterobacteriaceae was detected in the most of cicadas. Most of the Enterobacteriaceae species identified were biosafety level 2 pathogens or opportunistic pathogens. SPC and Enterobacteriaceae were distributed both on the surface and in the body of the cicadas. SPC and Enterobacteriaceae rapidly increased in the dead cicadas during the storage (at 25 degrees C). In this study, it is concluded that sufficient heat cooking and proper cold storage should be necessary for the safe consumption of cicadas as food., BRILL
JOURNAL OF INSECTS AS FOOD AND FEED, 2024年05月, ［査読有り］

加熱不十分な鶏肉製品に対する調理前の高圧処理による食中毒菌低減効果の検討　　　　　　　　　　　　　　　
岡田由美子; 鈴木穂高; 筒浦さとみ; 西海理之; 百瀬愛佳; 野田衛
日本食品微生物学会雑誌, 2024年03月, ［査読有り］

Microbiological Assessment of Pasteurized Milk in Japan by Different Testing Methods
Hodaka Suzuki; Amalia Widya Rizky Sahoed; Rikako Midoh; Yumiko Okada, 筆頭著者, Science and Education Publishing Co., Ltd.
Journal of Food and Nutrition Research, 2023年03月, ［査読有り］

Impacts of Subchronic and Mild Social Defeat Stress on Plasma Putrefactive Metabolites and Cardiovascular Structure in Male Mice
Atsushi Toyoda; Kina Kawakami; Yuto Amano; Hideaki Nishizawa; Shin-ichi Nakamura; Takahiro Kawase; Yuta Yoshida; Hodaka Suzuki; Takamitsu Tsukahara, Psychosocial stress precipitates mental illnesses, such as depression, and increases the risk of other health problems, including cardiovascular diseases. In this study, we observed the effects of psychosocial stress on the histopathological features of systemic organs and tissues in a mouse psychosocial stress model, namely the subchronic and mild social defeat stress (sCSDS) model. There were several pathological findings in the tissues of both sCSDS and control mice. Mild fibrosis of the heart was observed in sCSDS mice but not in control mice. Extramedullary hematopoiesis in the spleen and hemorrhage in the lungs were observed in both the control and sCSDS mice. Focal necrosis of the liver was seen only in control mice. Furthermore, putrefactive substances in the blood plasma were analyzed because these metabolites originating from intestinal fermentation might be linked to heart fibrosis. Among them, plasma p-cresyl glucuronide and p-cresyl sulfate concentrations significantly increased owing to subchronic social defeat stress, which might influence cardiac fibrosis in sCSDS mice. In conclusion, several pathological features such as increased cardiac fibrosis and elevated plasma putrefactive substances were found in sCSDS mice. Thus, sCSDS mice are a potential model for elucidating the pathophysiology of psychosocial stress and heart failure., MDPI AG
International Journal of Molecular Sciences, 2023年01月, ［査読有り］

Drastic Hypothermia after Intraperitoneal Injection of Okadaic Acid, a Diarrhetic Shellfish Poisoning Toxin, in Mice.　　　　　　　　　　　　　　　
Suzuki; H., 筆頭著者
Exp. Anim., 2021年08月, ［査読有り］

Anterior Segment Dysmorphogenesis of the Eye and Glaucoma in MG-W Gerbils.　　　　　　　　　　　　　　　
Fukunaga; Y.; Ogawa; T.; Suzuki; H.; Okada; Y.; Nakazawa; T.; Yamaguchi; Y.
J. Toxicol. Pathol., 2021年07月, ［査読有り］

Comparison of the Susceptibility to Tetrodotoxin in ddY Strain Mice from Different Breeder Companies.　　　　　　　　　　　　　　　
Suzuki, H, 筆頭著者
Food Hyg. Saf. Sci., 2020年02月, ［査読有り］

Prevalence of Cronobacter spp. in Retail Foods and Farm-Associated Environments in Japan.　　　　　　　　　　　　　　　
H. Ogihara; N. Fukuda; A. Fujiwara; H. Suzuki; H. Yamamoto; D. Kyoui; Y. Okada; S. Igimi
Food Sci. Tech. Res., 2019年04月, ［査読有り］

Comparative Toxicity of Dinophysistoxin-1 and Okadaic Acid in Mice.
H. Suzuki; Y. Okada, 筆頭著者, The mouse bioassay for diarrhetic shellfish poisoning toxins has been used worldwide. In this study, dinophysistoxin-1 (DTX-1) and okadaic acid (OA) were compared for toxicity. The lethality rate increased and the median survival time decreased in a dose-dependent manner in both DTX-1 and OA. The median lethal dose value was 150.4 µg/kg (95% confidence interval=130.1–171.2 µg/kg) for DTX-1 and 185.6 µg/kg (95% confidence interval=161.2–209.6 µg/ kg) for OA. The toxicity equivalent factor 1:1 has been used for OA and DTX-1 in the EU and Japan. Thus, it may be considered that toxicity potential of DTX-1 has remained underestimated as compared to that of OA and DTX-1 might be more toxic than OA., Japanese Society of Veterinary Science
J. Vet. Med. Sci., 2018年04月, ［査読有り］

Effects of High Hydrostatic Pressure Processing on the Number of Bacteria and Texture of Beef Liver.
H. Ogihara; H. Suzuki; M. Michishita; H. Hatakeyama; Y. Okada, 責任著者, Providing beef liver for raw consumption was banned in Japan on July 1, 2012. To lift the ban, the establishment of effective countermeasures for safe raw consumption is necessary. In this study, we examined the effects of high hydrostatic pressure processing on raw beef liver. Beef liver samples subjected to 300MPa of pressure or higher for 10min at 25 degrees C became firmer and showed a paler color and were considered unsuitable for raw consumption. More than 3.0 log reductions of bacteria were seen after treatments at 400 and 500MPa, but the treatment with lower pressure did not show enough microcidal effects for safe consumption. Histological and ultrastructural analysis revealed that high hydrostatic pressure processing increased mitochondrial swelling and reduced rough endoplasmic reticula in hepatocytes, and such changes might be related to the observed changes of texture in the treated raw beef liver., WILEY-HINDAWI
J. Food Qual., 2017年05月, ［査読有り］

Differences in susceptibility of mouse strains to tetrodotoxin
Hodaka Suzuki, 筆頭著者, The mouse bioassay for tetrodotoxin has been used for many years in Japan. To the best of our knowledge, however, there have only been a few reports that have specifically investigated differences in susceptibility to tetrodotoxin among mouse strains. In this study, we investigated the response of various mouse strains to tetrodotoxin. Tetrodotoxin solution was injected intraperitoneally into male mice of 5 inbred strains (A/J, BALB/c, C3H/He, C57BL/6, and DBA/2) and male and female mice of 2 non-inbred strains (ddY and ICR). Significant differences in susceptibility to tetrodotoxin were found among the mouse strains tested. In comparison to the ddY male mice, which are designated to be used in the Japanese reference method, the 5 inbred strains of mice tested were significantly more resistant to tetrodotoxin. However, no significant differences in tetrodotoxin susceptibility were observed between ddY male and female mice or between ddY male mice and ICR male and female mice. These results indicate that the users of the mouse bioassay should pay attention to differences in mouse strain in susceptibility to tetrodotoxin. (C) 2016 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
TOXICON, 2016年09月, ［査読有り］

水晶体脱臼を伴う眼球腫大のスナネズミ4症例　　　　　　　　　　　　　　　
小川竜也; 岡田由美子; 久世博; 花見正幸; 鈴木穂高, ラスト(シニア)オーサー
比較眼科研究, 2015年10月, ［査読有り］

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated from the Imported and the Domestic Foods in Japan.　　　　　　　　　　　　　　　
Y. Okada; S. Monden; H. Suzuki; A. Nakama; M. Ida; S. Igimi
J. Food Nutr. Sci., 2015年01月, ［査読有り］

Comparison of Toxicity between Saxitoxin and Decarbamoyl Saxitoxin in the Mouse Bioassay for Paralytic Shellfish Poisoning Toxins
Hodaka Suzuki; Kenji Machii, 筆頭著者, The mouse bioassay (MBA) for paralytic shellfish poisoning (PSP) toxins has been used in the AOAC Official Method and the official Japanese method. In the AOAC Official Method, the saxitoxin (STX) standard provided by the U.S. Food and Drug Administration (FDA) is used, but no standard is used in the official Japanese method. The objective of this study was to compare the toxicity of decarbamoyl STX (dcSTX), one of the derivatives of STX and a candidate standard for the MBA for PSP toxins in Japan, to that of FDA STX in the MBA platform. In this study, the toxicity of dcSTX was 918.0 +/- 44.9 mouse units/mu mol, and the relative toxicity ratio of deSTX to FDA STX based on moles was 0.478., JAPAN SOC VET SCI
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2014年11月, ［査読有り］

Influence of body weight of mice on the susceptibility to okadaic acid, a diarrhetic shellfish poisoning toxin
Hodaka Suzuki, 筆頭著者, The mouse bioassay (MBA) for diarrhetic shellfish poisoning (DSP) toxins has been widely used in many countries of the world. However, different body weight ranges of mice are designated to be used in the Japanese official method and European Union procedure. In this study we investigated whether and to what extent the body weights of the mice affect the susceptibility to DSP toxins. A lethal dose of okadaic acid, one of the representative DSP toxins, was injected intraperitoneally into mice of five different body weight range groups, from 14 to 24g. The mice were observed until 24h after injection. The lethality was 100% in the 14-15 and 16-17g groups, 80% in the 19-20g group, 50% in the 21-22g group, and 40% in the 23-24g group, with significant differences. Survival analysis indicated a relationship between body weights of mice and susceptibility to okadaic acid. These results would be quite useful not only for the MBA, but also to improve understanding of the biological responses to DSP toxins., TAYLOR & FRANCIS LTD
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2014年04月, ［査読有り］

Bacterial Translocation in Alymphoplasia (aly/aly) Mice
Hodaka Suzuki; Yumiko Okada, 筆頭著者, Bacterial translocation (BTL) is defined as the passage of viable bacteria from the gastrointestinal tract to the organs. This study was to elucidate the roles of Peyer's patches (PPs) and/or mesenteric lymph nodes (MLNs) in BTL. Alymphoplastic mutant mice and phenotypically normal heterozygous mice were dominantly colonized with streptomycin-resistant Escherichia colt and BTL was examined. In PP- and MLN-competent mice, BTL to MLNs was detected in 100% of mice, but BTL to organs was rare (25%). On the other hand, in PP- and MLN-deficientmice, BTL to organs was detected in 91% of mice. The results clearly indicate that PPs are not the only site for bacterial entry., POLISH ACAD SCIENCES, INST SYSTEMATICS EVOLUTION ANIMALS
FOLIA BIOLOGICA-KRAKOW, 2014年, ［査読有り］

Growth of Listeria monocytogenes in refrigerated ready-to-eat foods in Japan
Yumiko Okada; Izumi Ohnuki; Hodaka Suzuki; Shizunobu Igimi, The ability of L. monocytogenes to grow in a series of Japanese ready-to-eat (RTE) foods, including boiled baby sardine and Japanese pickle, was tested at two different refrigeration temperatures. In RTE foods in which L. monocytogenes can grow, growth was significantly higher at 10°C than that at 4°C during their shelf lives and growth patterns varied extensively among the different types of foods. However, growth did not occur at 4°C within the shelf life of certain RTE foods, such as broiled squid. The patterns of growth were varied extensively with different sample types. These results suggest that some types of traditional Japanese RTE foods stored at 10°C may be potential sources of listeriosis. To reduce the risk of food-borne listeriosis, studies to determine the contamination levels in RTE foods and the effects of storage temperature on their shelf lives are needed. © 2013 Copyright Taylor and Francis Group, LLC.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 2013年08月, ［査読有り］

Sequence of busulfan-induced neural progenitor cell damage in the fetal rat brain
Toko Ohira; Ryo Ando; Yumiko Okada; Hodaka Suzuki; Tsubasa Saito; Tomomi Nakazawa; Kaori Nishihara; Satoshi Yamamoto; Norihiko Nakamura; Kazutoshi Tamura, The sequence of neural progenitor cell (NPC) damage induced in fetal rat brain by transplacental exposure to busulfan, an antineoplastic bifunctional-alkylating agent, on gestational day 13 was examined by immunohistochemical and real-time RT-PCR analyses. Following busulfan treatment, pyknotic NPCs first appeared in the medial layer and then extended to the dorsal layer of the ventricular zone (VZ) of the telencephalon. Pyknotic NPCs that were immunohistochemically positive for cleaved caspase-3, i.e. apoptotic NPCs, began to increase at 24. h after treatment, peaked at 48. h, and returned to the control levels at 96. h. On the other hand, the index (%) of phospho-histone H3-positive NPCs, i.e. mitotic NPCs, and that of BrdU-positive NPCs, i.e. S-phase cells, decreased in accordance with the increase in the index of apoptotic NPCs. Prior to the peak time of apoptotic NPCs, the indices of p53- and p21-positive NPCs peaked at 36. h. In addition, the expression levels of p21 and Puma (p53-target genes) mRNAs were elevated in real-time RT-PCR analysis. These findings indicated that busulfan not only induced apoptosis through the p53-mediated intrinsic pathway but also inhibited cell proliferation in NPCs, resulting in a reduction of the width of the telencephalon. On the other hand, in spite of up-regulation of p21 expression, the expression of cyclin D1, part of the cell cycle machinery of the G1/S transition, and the expression levels of Cdc20 and cyclin B1 which are involved in G2/M transition, showed no changes, giving no possible information of busulfan-induced cell cycle arrest in NPCs. © 2012 Elsevier GmbH.
Experimental and Toxicologic Pathology, 2013年07月, ［査読有り］

Differences in Susceptibility to Okadaic Acid, a Diarrhetic Shellfish Poisoning Toxin, between Male and Female Mice
Hodaka Suzuki, 筆頭著者, The mouse bioassay (MBA) for diarrhetic shellfish poisoning (DSP) toxins has been widely used in many countries of the world. In the Japanese and EU methods, male mice are designated to be used for MBA. Female mice were described to be less susceptible than male mice. To the best of our knowledge, however, there have been no reports on the details of sex differences in susceptibility to DSP toxins. In this study, we investigated whether, and to what extent, female mice are less sensitive to DSP toxins. A lethal dose of okadaic acid (OA), one of the representative DSP toxins, was injected intraperitoneally into mice. The mice were observed until 24 hours after injection. Both male and female mice of ICR and ddY strains, which are designated in the Japanese official method, were compared. All the mice were four weeks old and weighed 18-20 g. The experiments were repeated twice. The lethality was 70%-100%. Survival analysis showed no sex differences in susceptibility to OA, but ICR female mice showed significant resistance compared with other groups in one out of two trials. These results indicate that sex differences were not clear but, nonetheless, male mice showed more stable results., MDPI AG
TOXINS, 2013年01月, ［査読有り］

Effects of injection speed of test samples on the mouse bioassay for paralytic shellfish poisoning toxins
Hodaka Suzuki; Kenji Machii, 筆頭著者, The mouse bioassay has been used as the official method for paralytic shellfish poisoning toxins detection in Japan since 1980. However, differences in the results of this assay, when performed by different investigators, have been noted despite the use of the same sample. This study was performed to examine the effect of the injection speed, a hypothetical cause of such differences, on the death time of mice. Speed-controlled injection of the toxin (at 12, 6, 3, and 1.5 mL/min) into mice was performed using a syringe pump, and the death times of mice were measured. No statistically significant differences were found among the groups, even between fast injection (5 s) and very slow injection (40 s), indicating that the injection speed may not be the crucial factor for this assay. © H. Suzuki and K. Machii, 2013 Licensee PAGEPress, Italy.
Italian Journal of Food Safety, 2013年, ［査読有り］

Age-dependent changes in intraepithelial lymphocytes (IELs) of the small intestine, cecum, and colon from young adult to aged mice
Hodaka Suzuki, 筆頭著者, We previously reported the regional differences in the IELs present in the proximal (P), middle (M), and distal (D) parts of the small intestine, cecum (Ce), and colon (Co) of mice. In this study, we investigated the age-dependent changes in the regional differences of IELs from young adult to aged mice. In this experiment, 3-, 6-, 12-, 18-, and 24-month-old mice were examined. IELs were separately isolated from 5 parts of the intestines and analyzed by flow cytometry. Regional differences in the number and phenotype of IELs showed the same trends in all age groups. The number of IELs was highest in 6-month-old mice and then gradually decreased with age. As to IEL subsets, age-related changes were not seen except for a few subsets among the age groups. We conclude that age-related decreases in IELs in mouse small intestine may be one of the aging phenomena of the intestinal immune system. Such age-related decreases in IELs may be concerned with the increased liability to intestinal infections in the elderly. (C) 2011 Elsevier Ireland Ltd. All rights reserved., ELSEVIER IRELAND LTD
ARCHIVES OF GERONTOLOGY AND GERIATRICS, 2012年09月, ［査読有り］

Rapid and Drastic Decreases of Body Temperature in Mice Intraperitoneally Injected with Lethal Dose of Okadaic Acid.　　　　　　　　　　　　　　　
H. Suzuki, 筆頭著者
Proceedings of the 14th ICHA, 2012年07月

Susceptibility of different mice strains to okadaic acid, a diarrhetic shellfish poisoning toxin
Hodaka Suzuki, 筆頭著者, The mouse bioassay is widely used to detect diarrhetic shellfish poisoning (DSP) toxins. To the best of our knowledge, however, there have been no reports specifically on strain differences in susceptibility to DSP toxins. In this study, we investigated the susceptibility of different mice strains to okadaic acid (OA), one of the representative DSP toxins. A lethal dose of OA was injected intraperitoneally (i.p.) into mice. The mice were observed until 24 h after injection. Five inbred strains (A/J, BALB/c, C3H/He, C57BL/6, and DBA/2) and two non-inbred strains (ddY, and ICR) of mice were compared. All the mice were male, weighed 16-20 g, and were 4-5 weeks old. The lethality was 90-100% in the A/J, BALB/c, ddY, and ICR strains, 70-80% in the C3H/He and C57BL/6 strains, and 40% in DBA/2 strain. Survival analysis showed that the BALB/c, C57BL/6, ddY, and ICR strains died earlier and the A/J, C3H/He and DBA/2 strains survived longer. These results indicate that significant differences may exist in the susceptibility of mice strains to OA., TAYLOR & FRANCIS LTD
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2012年, ［査読有り］

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan
Yumiko Okada; Akiko Okutani; Hodaka Suzuki; Hiroshi Asakura; Shuko Monden; Akiko Nakama; Tsutomu Maruyama; Shizunobu Igimi, The antimicrobial susceptibility of 201 Listeria monocytogenes isolates from foods, environments, animals and human patients in Japan was determined. All isolates were susceptible to ampicillin, the first choice of drug for listeriosis treatment, chloramphenicol, dihydrostreptomycin, erythromycin, enrofloxacin, gentamicin, kanamycin, lincomycin, nosiheptide, salinomycin, vancomycin, and virginiamycin. A human strain was resistant to oxytetracycline. The Minimum Inhibitory Concentration (MIC) for 50% of the strains and the MIC for 90% of the strains were comparable in all the isolates. This is the first investigation to compare antibiotic resistances between isolates from foods and isolates from human patients in Japan. The result showed that most of the isolates were susceptible to antibiotics used in this study., JAPAN SOC VET SCI
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2011年12月, ［査読有り］

Differences in Gene expression Profiles among the Proximal, Middle and Distal Peyer’s Patches in the Mouse Small Intestine.
H. Suzuki; R. Ohtsuka; M. Takeda, 筆頭著者, Approximately 6-11 Peyer's patches are present in the mouse small intestine. However, to the best of our knowledge, few studies have reported the regional differences in mouse Peyer's patches. This study aims to determine whether regional differences exist in the immunological activation status and immunological functions of mouse Peyer's patches in the normal state. The most proximal Peyer's patches, the most distal Peyer's patches and the Peyer's patches nearest to the midpoint between the pylorus and ileocecal junction were obtained from the mouse small intestine. The gene expression levels in the PPs obtained from different regions were compared using the DNA microarray technique. Of the 187 genes that were expressed differently among the Peyer's patches from different regions, 6 genes were related to immune system process. The 6 genes that significantly differed in their expression levels were histocompatibility 2, q region locus 1 (H2-Q1)
interleukin-18 (1l18)
adenosine disaminase (Ada)
angiogenin, ribonuclease A family, member 4 (Ang4)
chemokine (c-c motif) ligand 6 (Ccl6) and histocompatibility 2, T region locus 22 (H2-T22). These findings suggest that the regional differences among Peyer's patches in mice in terms of the immunological activation status and immunological functions may be subtle. © 2011 Asian Network for Scientific Information.
Res. J. Immunol., 2011年, ［査読有り］

Quantitative Risk Assessment of Vibrio parahaemolyticus in Finfish: A Model of Raw Horse Mackerel Consumption in Japan
Jun'ichiro Iwahori; Akio Yamamoto; Hodaka Suzuki; Takehisa Yamamoto; Toshiyuki Tsutsui; Keiko Motoyama; Mikiko Sawada; Tomoki Matsushita; Atsushi Hasegawa; Ken Osaka; Hajime Toyofuku; Fumiko Kasuga, The aim of this study was to evaluate the effects of implemented control measures to reduce illness induced by Vibrio parahaemolyticus (V. parahaemolyticus) in horse mackerel (Trachurus japonicus), seafood that is commonly consumed raw in Japan. On the basis of currently available experimental and survey data, we constructed a quantitative risk model of V. parahaemolyticus in horse mackerel from harvest to consumption. In particular, the following factors were evaluated: bacterial growth at all stages, effects of washing the fish body and storage water, and bacterial transfer from the fish surface, gills, and intestine to fillets during preparation. New parameters of the beta-Poisson dose-response model were determined from all human feeding trials, some of which have been used for risk assessment by the U.S. Food and Drug Administration (USFDA). The probability of illness caused by V. parahaemolyticus was estimated using both the USFDA dose-response parameters and our parameters for each selected pathway of scenario alternatives: washing whole fish at landing, storage in contaminated water, high temperature during transportation, and washing fish during preparation. The last scenario (washing fish during preparation) was the most effective for reducing the risk of illness by about a factor of 10 compared to no washing at this stage. Risk of illness increased by 50% by exposure to increased temperature during transportation, according to our assumptions of duration and temperature. The other two scenarios did not significantly affect risk. The choice of dose-response parameters was not critical for evaluation of control measures., WILEY-BLACKWELL PUBLISHING, INC
RISK ANALYSIS, 2010年12月, ［査読有り］

Differences in Intraepithelial Lymphocytes in the Proximal, Middle, Distal Parts of Small Intestine, Cecum, and Colon of Mice
Hodaka Suzuki, 筆頭著者, We have previously reported the regional differences in the intraepithelial lymphocytes (IELs) present in the small intestine of mice. In this study, we further investigated these differences on the basis of our previous findings and studied the entire intestine, including the cecum and colon. Most of the significant differences in phenotypic compositions were found between the small and large intestines, although some differences were found among the different parts of the small and large intestines. In particular, the composition of the subsets in alpha beta T cells and gamma delta T cells clearly differed between the small and large intestines. For example, in alpha beta T cells, the percentages of double negative (DN) and CD8 alpha alpha(+) cells were higher in the large intestine, that of CD8 alpha beta(+) cells was higher in the small intestine, and those of CD4(+) and CD4(+) CD8 alpha alpha(+) double positive (DP) cells were higher in the distal part of the small intestine. In gamma delta T cells, the percentage of CD8 alpha alpha(+) cells was higher in the small intestine and that of DN cells was higher in the large intestine. These results indicate that the differences between IELs in the small and large intestines are discontinuous., TAYLOR & FRANCIS INC
IMMUNOLOGICAL INVESTIGATIONS, 2009年, ［査読有り］

米国産輸入牛肉と我が国の腸管出血性大腸菌による食中毒，および感染症発生に関する研究
鈴木穂高; 山本茂貴, 筆頭著者, 国立医薬品食品衛生研究所安全情報部
国立医薬品食品衛生研究所報告, 2008年12月, ［査読有り］

Regional variations in the distributions of small intestinal intraepithelial lymphocytes (IELs) in outbred laboratory mice (Mus musculus domesticus) and the inbred strain of mice established from Japanese fancy mice (Mus musculus molossinus)
H Suzuki; S Yamamoto, 筆頭著者, Previously, we reported regional variations in small intestinal IELs of mice. In this study, we examined the regional variations of IELs in outbred laboratory mice (ddY: Mus musculus domesticus) and the inbred strain of mice established from Japanese fancy mice (JF1: Mus musculus molossinus). IELs were isolated from the proximal, middle and distal parts of the small intestine and analyzed by flow cytometry. The percentages of gamma delta T cells and alpha beta T cell Subset of extrathymic origin were higher in the proximal part while the percentages of alpha beta T cell subset(s) of thymic origin were higher in the distal part in both ddY and JF1 mice. Such trends in regional variations of IELs were almost the same as those found in the inbred strains of laboratory mice in our previous reports. This strongly suggests that these regional variations of IELs may be common phenomena in Mus muscultis species. (c) 2005 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2006年, ［査読有り］

Development of early apoptosis and changes in T-cell subsets in mouse thymocyte primary cultures treated with nivalenol
A Poapolathep; S Kumagai; H Suzuki; K Doi, Nivalenol (NIV), a trichothecene mycotoxin, is a secondary fungal metabolite mainly produced by Fusarium nivale. We first reported that NIV could induce apoptosis and changes in lymphocyte subsets in lymphoid tissues of mice. In this study, to clarify the direct effects of NIV on thymocytes, mouse thymocyte primary cultures were treated with NIV at the dose levels of 0.25, 0.5 and 1.0 mug/ml and examined for up to 24 h after treatment by flow cytometry. The number of viable cells decreased significantly at and after 6 It in dose- and time-dependent manners, and FACS analysis revealed that the apoptotic cell index showed a significant increase in all treated groups at and after 3 h in a time-dependent manner. The index at 24 h was lowest in 1.0 mug/ml-group. The number of CD4(+)CD8(+) cells was prominently depleted in all groups in a time-dependent manner. On the other hand, the numbers of CD4(+)CD8(-) and CD4(-)CD8(+) cells were significantly depleted only in 1.0 mug/ml-group at 24 HAT. These results indicate that NIV directly affects thymocytes and induces apoptosis mainly in CD4(+)CD8(+) cells. (C) 2004 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2004年10月, ［査読有り］

Development of early apopotosis and changes in lymphocyte subsets in lymphoid organs of mice orally inoculated with nivalenol
A Poapolathep; T Nagata; H Suzuki; S Kumagai; K Doi, Development of early apoptosis and changes in lymphocyte subsets were examined in lymphoid organs of female BALB/c mice after oral administration of 15 mg/kg b.w. of nivalenol (NIV), the major type B trichothecene mycotoxin, by FACS analysis. Judging from the results of viable cell count and apoptotic cell index, NIV attacked Peyer's patches first and thymus most severely. In thymus, selective damage in CD4(+)CD8(+) cells was observed at 12 and 24 It after inoculation (HAI), following the peak of apoptosis at 9 HAI. CD4(+) cells were clearly suppressed at 3 HAI in Peyer's patches, at and after 9 HAI in mesenteric lymph nodes, and 3 to 12 HAI in spleen, respectively. CD8(+) cells were also suppressed at 24 HAI in mesenteric lymph nodes and at 12 HAI in spleen, respectively. As to changes in B cell subsets, IgG(+) cells significantly decreased from 3 to 12 HAI and all B cell subsets at 24 HAI in mesenteric lymph nodes. In spleen, IgM(+) cells were suppressed at 9 HAI. On the other hand, in Peyer's patches, following clear decrease in the numbers of pan-T and pan-B cells and viable cells at 3 HAI, all B cell subsets, especially IgA(+) cells, showed a significant increase in their numbers at 9 HAI, and the numbers of IgA(+) and IgM(+) cells remained higher values than controls thereafter. Taken together, in the course of recovery from NIV-induced prominent damage in Peyer's patches at 3 HAI, interaction of NIV with Peyer's patches might result in in vivo stimulation of interleukin production at this site and result in increased proliferation and differentiation of IgA-secreting B cells at and after 9 HAI. (C) 2003 Elsevier Science (USA). All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2003年08月, ［査読有り］

Age-related changes in the regional variations in the number and subsets of intraepithelial lymphocytes in mouse small intestine
H Suzuki; KI Jeong; K Doi, 筆頭著者, Previously, we reported regional variations in the number and subsets of the small intestinal IELs of mice. In this study. we examined the age-related changes in the regional variations of IELs in mice front 2 to I I weeks old. IELs were isolated from the proximal, middle and distal parts of the small intestine and analysed by flow cytometry. The total number of IELs gradually increased with age and reached a plateau at 8 weeks old, As to IEL subsets. the percentage of a 0 T cells was higher in the distal part at and after 2 weeks of age (before weaning). The percentage of the alphabeta T cell subset of extrathymic origin was higher in the proximal part while the percentages of alphabeta T cell subsets of thymic origin were higher in the distal part at and after 3 weeks (just after weaning). It appears that regional variations in IELs may be formed before the weaning period in mice. (C) 2002 Published by Elsevier Science Ltd., PERGAMON-ELSEVIER SCIENCE LTD
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2002年07月, ［査読有り］

Regional variations in the distributions of small intestinal Intraepithelial lymphocytes in germ-free and specific pathogen-free mice
H Suzuki; KI Jeong; K Itoh; K Doi, 筆頭著者, Previously, we reported the regional variations in intraepithelial lymphocytes (IELs) in the small intestine of mice. To clarify the effects of intestinal bacteria on the distribution of IELs, regional variations in IELs were examined using germ-free (GF) and specific pathogen-free (SPF) BALB/cA mice. The small intestine was taken and divided equally into three parts the proximal, middle, and distal parts). IELs were isolated from each part of the intestine, and the total number of IELs in GF mice was about one seventh of that in SPF mice. The decreased number of IELs in GF mice suggests that intestinal bacteria may be essential for local expansion of IELs. On the other hand, similar regional variations in IEL subsets observed it. both GF and SPF mice, except for some subsets. The similarity of regional variations in GF and SPF mice indicates that the regional variations In EEL subsets may not fundamentally depend on intestinal bacteria. (C) 2002 Elsevier Science (USA)., ACADEMIC PRESS INC ELSEVIER SCIENCE
EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2002年06月, ［査読有り］

CD8(+)-T-cell depletion ameliorates circulatory shock in Plasmodium berghei-infected mice
WL Chang; SP Jones; DJ Lefer; T Welbourne; G Sun; LJ Yin; H Suzuki; J Huang; DN Granger; HC van der Heyde, The Plasmodium berghei-infected mouse model is a well-recognized model for human cerebral malaria. Mice infected with P. berghei exhibit (i) metabolic acidosis (pH < 7.3) associated with elevated plasma lactate concentrations, (ii) significant (P < 0.05) vascular leakage in their lungs, hearts, kidneys, and brains, (ii) significantly (P < 0.05) higher cell and serum glutamate concentrations, and (iv) significantly (P < 0.05) lower mean arterial blood pressures. Because these complications are similar to those of septic shock, the simplest interpretation of these findings is that the mice develop shock brought on by the P. berghei infection. To determine whether the immune system and specifically CD8(+) T cells mediate the key features of shock during P. berghei malaria, we depleted CD8(+) T cells by monoclonal antibody (mAb) treatment and assessed the complications of malarial shock. P. berghei-infected mice depleted of CD8(+) T cells by mAb treatment had significantly reduced vascular leakage in their hearts, brains, lungs, and kidneys compared with infected controls treated with rat immunoglobulin G. CD8-depleted mice were significantly (P < 0.05) protected from lactic acidosis, glutamate buildup, and diminished HCO3- levels. Although the blood pressure decreased in anti-CD8 mAb-treated mice infected with P. berghei, the cardiac output, as assessed by echocardiography, was similar to that of uninfected control mice. Collectively, our results indicate that (i) pathogenesis similar to septic shock occurs during experimental P. berghei malaria, (ii) respiratory distress with lactic acidosis occurs during P, berghei malaria, and (iii) most components of circulatory shock are ameliorated by depletion of CD8(+) T cells., AMER SOC MICROBIOLOGY
INFECTION AND IMMUNITY, 2001年12月, ［査読有り］

Regional Variations in the Distribution of Small Intestinal Intraepithelial Lymphocytes in Alymphoplasia (aly/aly) Mice and Heterozygous (aly/+) Mice.
H. Suzuki; K. I. Jeong; K. Doi, 筆頭著者, Regional variations in intraepithelial lymphocytes (IELs) in the small intestine were examined in alymphoplasia mutant (aly/aly) mice, which are characterized by the systemic absence of lymph nodes and Peyer's patches, and heterozygous (aly/+) mice. The small intestines were taken from 10 to 12-week-old mice and divided equally into 3 parts (the proximal, middle and distal parts). IELs were isolated from each part of the intestine and analyzed with a flow cytometer. The number of IELs in the distal part was significantly fewer in aly/aly mice compared with aly/+ mice, although the total number of small intestinal IELs were comparable between them. As to the IELs subsets, regional variations in alpha beta T cells and gamma delta T cells were observed in aly/+ mice, but they disappeared in aly/aly mice. However, regional variations in composition of alpha beta T cell subsets were similarly observed in both aly/aly mice and aly/+ mice. This indicates that, although not essential, mesenteric lymph nodes (MLN) and/or Peyer's patches may modify the regional variations in IELs., MARCEL DEKKER INC
Immunol. Invest., 2001年11月, ［査読有り］

Development of apoptosis and changes in lymphocyte subsets in thymus, mesenteric lymph nodes and PEYER'S patches of mice orally inoculated with T-2 toxin
T Nagata; H Suzuki; N Ishigami; J Shinozuka; K Uetsuka; H Nakayama; KN Doi, Development of apoptosis and changes in lymphocyte subsets were examined mainly by flow cytometer in thymus, mesenteric lymph nodes and PEYER'S patches of mice up to 24 hours after oral inoculation with T-2 toxin (10 mg/kg). T-2 toxin attacked PEYER'S patches first, then mesenteric lymph nodes, and finally thymus in relation to the course of enteric absorption of orally inoculated T-2 toxin. The degree of lymphocyte apoptosis was prominent in the thymus, moderate in the PEYER'S patches, and somewhat mild in the mesenteric lymph nodes, suggesting the difference in lymphocyte population susceptible to T-2 toxin. As to the changes in lymphocyte subsets, CD4(+) CD8(+) T cells were most sensitive to T-2 toxin, and CD4(+) CD8(-) T cells were more severely depressed than CD4(-) CD8(+) T cells in the thymus. In the mesenteric lymph nodes, CD3(+) cells was more clearly affected than CD19(+) cells, and the numbers of CD4(+) and CD8(+) cells were similarly decreased. In the PEYER'S patches, the numbers of CD3(+), CD19(+), CD4(+) and CD8(+) cells were unexceptionally decreased. In addition, among IgM(+), IgG(+) and IgA(+) B cells, the number of IA(+) B cells which are more important in the mucosal immunity was most severely affected., URBAN & FISCHER VERLAG
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, 2001年09月, ［査読有り］

Regional variations in the distributions of small intestinal intraepithelial lymphocytes (IELs) in BALB/c+/+, nu/+, and nu/nu mice
H Suzuki; KI Jeong; K Doi, 筆頭著者, Regional variations in intraepithelial lymphocytes (IELs) in the small intestine were examined in BALB/c +/+, nu/+, and nu/nu mice. The small intestine was obtained from 11- to 12-week-old mice and divided equally into three (proximal, middle, and distal) parts. The IELs were isolated from each part of the intestine, and the total numbers of IELs in nu/+ and nu/nu mice were about a fifth of those in +/+ mice. Regional variations in the distribution of the IEL alpha beta, but not the gamma delta T-cell subset were found by use of flow cytometry in +/+ and nu/+ mice. On the other hand, such differences were not found in nu/nu mice, suggesting that thymus-independent development of T cells is not different among regions. Different local expansion of thymus-dependent alpha beta T cells may cause the regional variations seen in the distribution of alpha beta T cell IELs in +/+ and nu/+ mice., AMER ASSOC LABORATORY ANIMAL SCIENCE
COMPARATIVE MEDICINE, 2001年04月, ［査読有り］

Respiratory Reflexes in Spontaneously Breathing Anesthetized Dogs in Response to Nasal Administration of Sevoflurane, Isoflurane, or Halothane.
T. Mutoh; A. Kanamaru; H. Suzuki; H. Tsubone; R. Nishimura; N. Sasaki, Objective-To characterize respiratory reflexes elicited by nasal administration of sevoflurane (Sevo), isoflurane (Iso), or halothane (Hal) in anesthetized dogs.
Animals-8 healthy Beagles.
Procedure-A permanent tracheostomy was created in each dog. Two to 3 weeks later, dogs were anesthetized by IV administration of thiopental and alpha -chloralose. Nasal passages were isolated such that inhalant anesthetics could be administered to the nasal passages while the dogs were breathing 100% O-2 via the tracheostomy. Respiratory reflexes in response to administration of each anesthetic at 1.2 and 2.4 times the minimum alveolar concentration (MAC) and the full vaporizer setting (5%) were recorded. Reflexes in response to administration of 5% of each anesthetic also were recorded following administration of lidocaine to the nasal passages.
Results-Nasal administration of Sevo, Iso, and Hal induced an immediate ventilatory response characterized by a dose-dependent increase in expiratory time and a resulting decrease in expired volume per unit of time. All anesthetics had a significant effect, but for Sevo, the changes were smaller in magnitude. Responses to administration of each anesthetic were attenuated by administration of lidocaine to the nasal passages.
Conclusions and Clinical Relevance-Nasal administration of Sevo at concentrations generally used for mask induction of anesthesia induced milder reflex inhibition of breathing, presumably via afferent neurons in the nasal passages, than that of Iso or Hal. Respiratory reflexes attributable to stimulation of the nasal passages may contribute to speed of onset and could promote a smoother induction with Sevo, compared with Iso or Hal., AMER VETERINARY MEDICAL ASSOC
Am. J. Vet. Res., 2001年03月, ［査読有り］

T-2 toxin-induced apoptosis and c-fos mRNA expression in Con A-stimulated mouse thymocyte primary cultures
Junko Shinozuka; Hodaka Suzuki; Shigeki Tsutsui; Hiroyuki Nakayama; Kunio Doi, The development of apoptosis and the expression of c-fos mRNA were investigated up to 24 hours after treatment (HAT) with 0.2 g/ml of T-2 toxin in Con A-stimulated primary thymocyte cultures prepared from BALB/c mice. Cell viability began to decrease from 1 to 3 HAT, and it was approximately 50% at 6 HAT. The level of cytoplasmic nucleosomes peaked at 3 HAT (about 2 times of control) and decreased thereafter. At 6 HAT, thymocytes showed irregular-shaped or fragmented nuclei. By RT-PCR, the level of c-fos mRNA began to increase within 1 HAT, and maintained a high level through the observation period. Preincubation with BAPTA/AM, a intracellular calcium ion chelator, markedly suppressed the expression of c-fos mRNA and the level of DNA fragmentation induced by T-2 toxin. Although less effective, preincubation with H-7, a protein kinase C (PKC) inhibitor, also depressed the above-mentioned two parameters. These findings indicate that, in mouse thymocyte primary cultures treated with T-2 toxin, c-fos may play an important role in the induction of apoptosis and the increase in intracellular calcium ion may be closely related with the expression of c-fos mRNA. In addition, these findings also suggest that PKC-dependent pathwav may be involved in T-2 toxin-induced thymocyte apoptosis., Japanese Society of Toxicologic Pathology
Journal of Toxicologic Pathology, 2001年, ［査読有り］

Regional Variations in the Distributions of Small Intestinal Intraepithelial Lymphocytes (IELs) in Three Inbred Strains of Mice.
H. Suzuki; K. I. Jeong; T. Okutani; K. Doi, 筆頭著者, The regional variation in the intraepithelial lymphocytes (IELs) in the small intestine was examined in BALB/c male and female mice and C3H/He and C57BL/6 male mice. The small intestines were taken from 11 to 12-week-old mice and divided equally into 3 parts (the proximal, middle and distal parts). IELs were isolated from each part of the intestine and analyzed with flow cytometer. The number of IELs was highest in the proximal part and lowest in the distal part. The distribution of IEL subsets was markedly different between the proximal and the distal parts, and that in the middle part showed the intermediate pattern. The percentage of alpha beta T cells were higher in the distal part. In alpha beta T cell subset, the percentage of CD8 alpha alpha T cells was higher in the proximal part, whereas those of CD4 and CD4CD8 alpha alpha double positive T cells were higher in the distal part. In gamma delta T cell subset, no regional variations were found. The regional variations in the number and subsets of IELs showed almost the same patterns between male and female BALB/c mice and similar patterns among three strains of mice. This strongly suggests that the regional variations in the small intestinal IELs are common to mouse species., JAPAN SOC VET SCI
J. Vet. Med. Sci., 2000年08月, ［査読有り］

Picryl chloride-induced allergic dermatitis in IQI/Jic female mice
M Ikeda; K Kuroki; H Suzuki; H Nakayama; J Saegusa; K Doi, IQI/Jic (IQI) mice are an ICR-derived inbred strain developed in Japan, and it is known that aged females of this strain develop allergic dermatitis of spontaneous nature. In the present study, young IQI female mice which were sensitized with picryl chloride (PCL) to the shaved skin of abdomen and then topically applied with PCL to the ear at 4, 11, 18 and 25 days after the sensitization were examined. The ear swelling response increased rapidly after the Ist application, peaked after the 2nd one, and then gradually decreased. Histopathologically, edema with inflammatory cell infiltration developed after the Ist application and progressed after the 2nd one. The number of mast cells, CD4-positive cells and MHC class II-positive cells became prominent accompanied with epidermal thickening and dermal fibroplasia after the 4th application when clear elevation of total serum IgE levels was observed in many mice. Compared with the dermatitis induced in the same way in BALB/c female mice, the nature was similar with each other but the degree was obviously severer in IQI female mice. IQI female mice are considered to be a useful laboratory animal for the investigation of allergic dermatitis., GUSTAV FISCHER VERLAG
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, 2000年06月, ［査読有り］

Ultrastructural study on the follicle-associated epithelium of nasal-associated lymphoid tissue in specific pathogen-free (SPF) and conventional environment-adapted (SPF-CV) rats
KI Jeong; H Suzuki; H Nakayama; K Doi, Membranous (M) cells in follicle-associated epithelium (FAE) play an important role in the mucosal immunity through transport of a variety of foreign antigens to the underlying mucosa-associated lymphoid tissue (MALT). We aimed to investigate the ultrastructure of M cells in the FAE covering nasal-associated lymphoid tissue (NALT) both in specific pathogen-free (SPF) rats and in conventional environment-adapted (SPF-CV) rats aged 8-38 wk. In NALT of both SPF and SPF-CV rats, FAE included the nonciliated microvillous cell, which appears to be an analogue of M cell previously described in other MALT. In SPF rats, M cells increased in number only slightly with age, and they maintained morphological uniformity irrespective of age. In SPF-CV rats, M cells selectively increased in number resulting in prominent expansion of FAE surface area in parallel with the duration of maintenance in a conventional environment. In addition, M cells in SPF-CV rats showed heterogeneity in their surface morphology such as the length and number of microvilli and cell surface area and outline. In addition, the FAE was stratified by various subtypes of M cells, which were characterised by several subcellular alterations including the presence of many keratin filaments, homogeneous dark bodies and extensive cytoplasmic interfoliation with wide intercellular spaces filled with amorphous proteinaceous material. These characteristics of M cells in SPF-CV rat were intimately related with a preferential influx of immunocompetent cells into the FAE, which was not seen or was very rare in SPF rats irrespective of age. The results suggest the possibility that NALT may effectively carry out the mucosal immune response against antigenic stimuli of different magnitude through the unique dynamics of M cells which seem to be influenced by the infiltration of immunocompetent cells., CAMBRIDGE UNIV PRESS
JOURNAL OF ANATOMY, 2000年04月, ［査読有り］

Regional variations in the number and subsets of intraepithelial lymphocytes in the mouse small intestine
H Suzuki; KI Jeong; T Okutani; K Doi, 筆頭著者, Background and Purpose: Regional variations in the intraepithelial lymphocytes (IELs) in the mouse small intestine were examined.
Methods: The small intestine was taken from six 12-week-old BALB/c mice and divided equally into three parts (proximal, middle, and distal). The IELs were isolated from each part of the intestine and analyzed by use of flow cytometry.
Results: The number of IELs in the distal part of the small intestine was significantly lower than that in the proximal and middle parts (P < 0.01). The distribution of IEL subsets was markedly different between the proximal and the distal parts, and that in the middle part had an intermediate pattern. The percentage of alpha beta T cells and expression of Thy-1.2 on the alpha beta T and gamma delta T cells were higher in the distal part (P < 0.01). The percentage of CD8 alpha alpha and of double-negative T cells were higher in the proximal part, whereas that of CD4CD8 alpha alpha double-positive T cells was higher in the distal part (P < 0.01). Regional variations were found mainly in the extrathymically developed T cell subsets.
Conclusion: Local development of IELs may differ between regions of the small intestine., AMER ASSOC LABORATORY ANIMAL SCIENCE
COMPARATIVE MEDICINE, 2000年02月, ［査読有り］

Regional variations in the number and subsets of intraepithelial lymphocytes in the mouse small intestine
Hodaka Suzuki; Kwang H Jeong; Taichi Okutani; Kunio Doi, 筆頭著者, Background and Purpose: Regional variations in the intraepithelial lymphocytes (IELs) in the mouse small intestine were examined. Methods: The small intestine was taken from six 12-week-old BALB/c mice and divided equally into three parts (proximal, middle, and distal). The IELs were isolated from each part of the intestine and analyzed by use of flow cytometry. Results: The number of IELs in the distal part of the small intestine was significantly lower than that in the proximal and middle parts (P<
0.01). The distribution of IEL subsets was markedly different between the proximal and the distal parts, and that in the middle part had an intermediate pattern. The percentage of αβT cells and expression of Thy-1.2 on the αβT and γδT cells were higher in the distal park (P<
0.01). The percentage of CD8αα and of double-negative T cells were higher in the proximal part, whereas that of CD4CD8αα double-positive T cells was higher in the distal part (P<
0.01). Regional variations were found mainly in the extrathymically developed T cell subsets. Conclusion: Local development of IELs may differ between regions of the small intestine.
Comparative Medicine, 2000年, ［査読有り］

Effects of Permanent Tracheostomy on Respiratory Reflexes to Lung Inflation and Capsaicin in Sevoflurane-Anaesthetized Dogs.
T. Mutoh; A. Kanamaru; H. Suzuki; R. Nishimura; N. Sasaki; H. Tsubone, The aim of this study was to evaluate the effects of permanent tracheostomy on baseline breathing, reflex responses to lung hyperinflation (1.5 kPa) and right atrial capsaicin injection (5 mu g/kg) before and at 3 and 5 weeks after tracheostomy in sevoflurane-anaesthetized spontaneously breathing dogs (n = 6). In all observation periods, apnoeic responses, represented by an increase in expiration time after lung inflation and right atrial capsaicin injection, were consistently observed to be a result of the Hering-Breuer inflation reflex and the pulmonary C-fibre chemoreflex. Investigation at 3 and 5 weeks after tracheostomy revealed no significant evidence of changing baseline breathing pattern or reflex responses to lung inflation and capsaicin in any ventilatory variable (inspiration time, expiration time, tidal volume, expired ventilation, and end-tidal PCO2) from levels recorded before surgery. These results indicate that permanent tracheostomy, at least up to 5 weeks, does not affect the baseline breathing pattern, the Hering-Breuer inflation reflex or the pulmonary C-fibre chemoreflex. Further, our investigation provides a useful canine model in respiratory physiology., WILEY
J. Vet. Med. A, 1999年08月, ［査読有り］

Diurnal changes in intraepithelial lymphocytes (IELs) in the small intestine of mice
H Suzuki; S Shibata; T Okutani; M Suzuki; M Nakayama; T Nishimura; K Doi, 筆頭著者, Diurnal changes in small intestinal intraepithelial lymphocytes (IELs) were examined in 8- to 10-week-old BALB/cA male mice. The ratio of T cell subsets expressing CD8 alpha alpha homodimer/CD8 alpha beta heterodimer was found to be higher in the dark period than that in the light period. Increased expression of Thy-1.2 on gamma delta T cells was also observed in the light period. No significant changes were found in other subsets. This is the first report to document diurnal changes in the small intestinal IELs in mice., INT PRESS EDITING CENTRE INC
EXPERIMENTAL ANIMALS, 1999年04月, ［査読有り］

Involvement of Macrophage Scavenger Receptors in Protection against Murine Malaria.
S. Nogami; J. Watanabe; K. Nakagami; K. Nakata; H. Suzuki; H. Suzuki; M. Fujisawa; T. Kodama; S. Kojima, Macrophage scavenger receptor A (MSR-A) deficient mice MSR-A(-/-) were infected by the intraperitoneal injection of the Plasmodium berghei NK65 strain in the erythrocytic stage. The MSR-A(-/-) mice died significantly earlier than the control mice (P = 0.060). In the surviving mice, two peaks of parasitemia were observed: the first 5-7 days and the second at 2-3 weeks after infection. Death of all MSR-A(-/-) mice occurred at either peak of parasitemia, suggesting that MSR-A protects mice from severe infection. This model may be useful for the study of molecular mechanisms of macrophage functions in malaria infection., AMER SOC TROP MED & HYGIENE
Am. J. Trop. Med. Hyg., 1998年11月, ［査読有り］

Age-related changes in susceptibility of mice to low-virulent mouse hepatitis virus (MHV-2-CC) infection
H Suzuki; W Kiatipattanasakul; S Kajikawa; S Tsutsui; H Nakayama; N Goto; K Doi, 筆頭著者, This study was performed to examine mouse age-dependent changes in susceptibility to MHV-2-CC-infection and participation of macrophages in such changes in BALB/c mice. One-week-old mice were fully susceptible (mortality, 100%), 2-week-old semi-susceptible (36%), and 3- and 4-week-old fully resistant (0%) to MHV-P-CC, respectively. Such age-dependent differences corresponded well with the differences in the virus titers in the liver, spleen and blood and in the severity of liver lesions. In 1-week-old mice with peritoneal exudate cells (PEG) transferred from 4-week-old mice and infected with MHV-2-CC, a slight prolongation of survival time was recorded, although there was no difference in mortality. In 3-week-old mice infected with MHV-2-CC after silica-treatment to suppress macrophages, there was no significant change in susceptibility. In macrophages infected with MHV-2-CC in vitro, the virus replicated better in macrophages obtained from younger mice. These results suggest that macrophages may play a small role in the age-related development of resistance to MHV-2-CC infection in BALB/c mice., JAPAN EXPER ANIMAL RES ASSN
EXPERIMENTAL ANIMALS, 1997年07月, ［査読有り］

Adrenocortical Carcinoma Treated with Adrenalectomy in a Dog.
T. Itoh; T. Kadosawa; M. Mochizuki; R. Nishimura; S. Matsunaga; H. Suzuki; H. Nakayama; N. Sasaki, 副腎機能充進症の発症前に診断された副腎皮質癌の13歳のイングリッシュセッターに対し, 副腎摘出術を行った。肉眼的に確認できた腫瘤は完全に摘出したが, この時点で反対側の副腎は外見上正常と思われた。しかしながら, ミネラルコルチコイド欠乏による術後の高カリウム血症が生じ, プレドニゾロン投与により改善された。手術の10ヵ月後, X線学的に肺転移が認められ, 身体検査および血液検査において副腎機能充進症に関連する異常が認められた。シスプラチンの投与により, 臨床症状の一時的な改善と血漿コルチゾール値の低下が認められたが, 2週間後に患犬は死亡した。剖検では, 肺, 脾臓, 肝臓, 腎臓, 小腸, および脳に転移病巣が認められた。, Japanese Society of Veterinary Anesthesia & Surgery
Jpn. J. Vet. Anesth. Surg., 1997年07月, ［査読有り］

熱帯性魚類食中毒シガテラのリスク評価のための研究
大城直雅; 登田美桜; 石川輝; 鈴木穂高; 豊福肇
食品衛生研究, 2018年05月, ［招待有り］

我が国の食肉の安全性，特に生食，加熱不足の危険性について
鈴木穂高
生活協同組合研究, 2017年08月, ［招待有り］
筆頭著者

【質疑応答】 臨床一般／法律・雑件：飛沫の飛ぶ距離は？ 対面調理時の衛生面への影響は？
鈴木穂高
週刊日本医事新報, 2016年07月, ［招待有り］
筆頭著者

日本、および諸外国における鶏卵・液卵のSalmonella汚染状況（文献調査）
鈴木穂高; 山本茂貴
国立医薬品食品衛生研究所報告, 2009年12月, ［査読有り］
筆頭著者

Campylobacter contamination in retail poultry meats and by-products in Japan: A literature survey
Hodaka Suzuki; Shigeki Yamamoto
FOOD CONTROL, 2009年06月, ［査読有り］
筆頭著者

Campylobacter Contamination in Retail Poultry Meats and By-Products in the World: A Literature Survey
Hodaka Suzuki; Shigeki Yamamoto
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2009年03月, ［査読有り］
筆頭著者

入門講座 国内の冷凍パン生地，ならびに原材料の汚染実態調査
鈴木穂高; 土屋禎; 田中廣行; 今溝洋子; 佐々木直; 森曜子; 五十君靜信; 豊福肇; 春日文子; 山本茂貴
食品衛生学雑誌, 2007年08月, ［査読有り］, ［招待有り］
筆頭著者

入門講座 小麦玄麦，小麦粉，および小麦粉を原料とする生地，生麺の微生物汚染に関する文献調査
鈴木穂高; 吉池由美子; 杉山恵; 長谷川専; 五十君静信; 豊福肇; 山本茂貴; 春日文子
食品衛生学雑誌, 2007年04月, ［査読有り］, ［招待有り］
筆頭著者

カキ摂食によるノロウイルス食中毒のリスク評価の試み
鈴木穂高; 春日文子
食品衛生研究, 2006年11月, ［招待有り］
筆頭著者

フレックネル実験動物の麻酔と鎮痛 第５版　　　　　　　　　　　　　　　
Paul Flecknel(著者)、笠井憲雪(訳者); 鈴木穂高(翻訳協力者), その他
株式会社アドスリー, 2023年12月
9784910513164

米国獣医学会 動物の安楽死指針（安楽死ガイドライン）：２０２０年版　　　　　　　　　　　　　　　
黒澤努、鈴木真 (鈴木穂高:翻訳協力), その他
株式会社アドスリー, 2022年05月
9784910513072

食品衛生検査指針 微生物編 改訂第2版 2018　　　　　　　　　　　　　　　
鈴木穂高, 共著
公益社団法人日本食品衛生協会, 2018年03月
9784889250985

食品衛生検査指針 微生物編 2015　　　　　　　　　　　　　　　
鈴木穂高, 共著
公益社団法人日本食品衛生協会, 2015年03月
9784889250725

Shellfish: Human Consumption, Health Implications and Conservation Concerns　　　　　　　　　　　　　　　
H. Suzuki, 共著
Nova Science Publishers, Inc., 2014年08月
9781633211957

リスク学用語小辞典　　　　　　　　　　　　　　　
鈴木穂高, 分担執筆
丸善, 2008年01月
9784621078495

Old Herborn University Seminar Monograph 19. Defense Mechanisms of the Innate System: Influence of Microbes　　　　　　　　　　　　　　　
J. J. Cebra; N. A. Bos; N. V. Boyko; N. Kushnir; M. Stoel; C. Surh; H. Suzuki; M. C. Thurnheer; G. D. Wu; K. Uchida; A. W. Zuercher, 分担執筆
Herborn Litterae, 2006年01月
3923022301