2021年05月, Animal Science Journal優秀論文賞, Social defeat models in animal science: What we have learned from rodent models, Animal Science Journal 88 944-952, 2017, 日本畜産学会
豊田 淳

2019年06月, 令和元年度国立大学法人茨城大学学長学術表彰優秀賞, 心理社会的ストレスモデル動物の栄養・代謝に関する研究, 国立大学法人茨城大学
豊田 淳

2019年03月, 日本畜産学会賞, 心理社会的ストレスモデル動物の栄養・代謝に関する研究, 日本畜産学会
豊田 淳
国内学会・会議・シンポジウム等の賞

2017年03月, 日本畜産学会Reviewers賞, 日本畜産学会
豊田 淳

Large-scale animal model study uncovers altered brain pH and lactate levels as a transdiagnostic endophenotype of neuropsychiatric disorders involving cognitive impairment.
Hideo Hagihara; Hirotaka Shoji; Satoko Hattori; Giovanni Sala; Yoshihiro Takamiya; Mika Tanaka; Masafumi Ihara; Mihiro Shibutani; Izuho Hatada; Kei Hori; Mikio Hoshino; Akito Nakao; Yasuo Mori; Shigeo Okabe; Masayuki Matsushita; Anja Urbach; Yuta Katayama; Akinobu Matsumoto; Keiichi I Nakayama; Shota Katori; Takuya Sato; Takuji Iwasato; Haruko Nakamura; Yoshio Goshima; Matthieu Raveau; Tetsuya Tatsukawa; Kazuhiro Yamakawa; Noriko Takahashi; Haruo Kasai; Johji Inazawa; Ikuo Nobuhisa; Tetsushi Kagawa; Tetsuya Taga; Mohamed Darwish; Hirofumi Nishizono; Keizo Takao; Kiran Sapkota; Kazutoshi Nakazawa; Tsuyoshi Takagi; Haruki Fujisawa; Yoshihisa Sugimura; Kyosuke Yamanishi; Lakshmi Rajagopal; Nanette Deneen Hannah; Herbert Y Meltzer; Tohru Yamamoto; Shuji Wakatsuki; Toshiyuki Araki; Katsuhiko Tabuchi; Tadahiro Numakawa; Hiroshi Kunugi; Freesia L Huang; Atsuko Hayata-Takano; Hitoshi Hashimoto; Kota Tamada; Toru Takumi; Takaoki Kasahara; Tadafumi Kato; Isabella A Graef; Gerald R Crabtree; Nozomi Asaoka; Hikari Hatakama; Shuji Kaneko; Takao Kohno; Mitsuharu Hattori; Yoshio Hoshiba; Ryuhei Miyake; Kisho Obi-Nagata; Akiko Hayashi-Takagi; Léa J Becker; Ipek Yalcin; Yoko Hagino; Hiroko Kotajima-Murakami; Yuki Moriya; Kazutaka Ikeda; Hyopil Kim; Bong-Kiun Kaang; Hikari Otabi; Yuta Yoshida; Atsushi Toyoda; Noboru H Komiyama; Seth G N Grant; Michiru Ida-Eto; Masaaki Narita; Ken-Ichi Matsumoto; Emiko Okuda-Ashitaka; Iori Ohmori; Tadayuki Shimada; Kanato Yamagata; Hiroshi Ageta; Kunihiro Tsuchida; Kaoru Inokuchi; Takayuki Sassa; Akio Kihara; Motoaki Fukasawa; Nobuteru Usuda; Tayo Katano; Teruyuki Tanaka; Yoshihiro Yoshihara; Michihiro Igarashi; Takashi Hayashi; Kaori Ishikawa; Satoshi Yamamoto; Naoya Nishimura; Kazuto Nakada; Shinji Hirotsune; Kiyoshi Egawa; Kazuma Higashisaka; Yasuo Tsutsumi; Shoko Nishihara; Noriyuki Sugo; Takeshi Yagi; Naoto Ueno; Tomomi Yamamoto; Yoshihiro Kubo; Rie Ohashi; Nobuyuki Shiina; Kimiko Shimizu; Sayaka Higo-Yamamoto; Katsutaka Oishi; Hisashi Mori; Tamio Furuse; Masaru Tamura; Hisashi Shirakawa; Daiki X Sato; Yukiko U Inoue; Takayoshi Inoue; Yuriko Komine; Tetsuo Yamamori; Kenji Sakimura; Tsuyoshi Miyakawa, Increased levels of lactate, an end-product of glycolysis, have been proposed as a potential surrogate marker for metabolic changes during neuronal excitation. These changes in lactate levels can result in decreased brain pH, which has been implicated in patients with various neuropsychiatric disorders. We previously demonstrated that such alterations are commonly observed in five mouse models of schizophrenia, bipolar disorder, and autism, suggesting a shared endophenotype among these disorders rather than mere artifacts due to medications or agonal state. However, there is still limited research on this phenomenon in animal models, leaving its generality across other disease animal models uncertain. Moreover, the association between changes in brain lactate levels and specific behavioral abnormalities remains unclear. To address these gaps, the International Brain pH Project Consortium investigated brain pH and lactate levels in 109 strains/conditions of 2294 animals with genetic and other experimental manipulations relevant to neuropsychiatric disorders. Systematic analysis revealed that decreased brain pH and increased lactate levels were common features observed in multiple models of depression, epilepsy, Alzheimer's disease, and some additional schizophrenia models. While certain autism models also exhibited decreased pH and increased lactate levels, others showed the opposite pattern, potentially reflecting subpopulations within the autism spectrum. Furthermore, utilizing large-scale behavioral test battery, a multivariate cross-validated prediction analysis demonstrated that poor working memory performance was predominantly associated with increased brain lactate levels. Importantly, this association was confirmed in an independent cohort of animal models. Collectively, these findings suggest that altered brain pH and lactate levels, which could be attributed to dysregulated excitation/inhibition balance, may serve as transdiagnostic endophenotypes of debilitating neuropsychiatric disorders characterized by cognitive impairment, irrespective of their beneficial or detrimental nature.
eLife, 2024年03月26日, ［査読有り］

Alpha-glycosyl isoquercitrin alleviates subchronic social defeat stress-induced depression symptoms by modulating the microbiota-gut-brain axis in mice.
Hong Liu; Junjie Li; Shogo Takahashi; Atsushi Toyoda; Ryo Inoue; Mihoko Koyanagi; Shim-Mo Hayashi; Meiyu Xu; Yuki Yamamoto; Kentaro Nagaoka, AIMS: Increasing evidence suggests a link between gut microbial dysbiosis and the pathogenesis of depression. Alpha-glycosyl isoquercitrin (AGIQ), consisting of isoquercitrin and its glycosylated quercetin, has beneficial effects on the gut microbiome and brain function. Here, we detected the potential antidepressant impact of a four-week administration of AGIQ and its underlying mechanisms using a mouse model of depression. MAIN METHODS: Male C57BL/6 mice were orally administered AGIQ (0.05 % or 0.5 % in drinking water) for 28 days; subchronic social defeat stress was performed in the last 10 days. Behavior tests were conducted to assess anxiety and depressive-like behaviors. Additionally, evaluations encompassed 5-hydroxytryptamine (5-HT) levels, the gut microbiota composition, lipopolysaccharide (LPS) concentrations, short-chain fatty acids levels, and intestinal barrier integrity changes. KEY FINDINGS: AGIQ significantly alleviated depression-like behaviors and increased hippocampal 5-HT levels. Further, AGIQ mitigated stress-induced gut microbial abnormalities and reduced the levels of LPS in the serum, which affected the relative gene expression levels of 5-HT biosynthesis enzymes in vitro. Furthermore, AGIQ reversed the reduced butyrate levels in cecal contents and improved the impaired intestinal barrier by increasing the expression of colonic zonula occluden-1 (ZO-1) and occludin, thereby decreasing LPS leakage. SIGNIFICANCE: Our results suggest that AGIQ could improve stress-induced depression by regulating the gut microbiome, which inhibits LPS production and maintains the gut barrier. This is the first report on the potential effect of AGIQ on depression via the gut microbiota-brain axis, shedding new light on treatment options.
Life sciences, 2024年03月13日, ［査読有り］

Effects of subchronic and mild social defeat stress on the intestinal microbiota and fecal bile acid composition in mice
Naoko YAMAGISHI; Daisuke KYOUI; Naoko MORIYA; Ayako AOKI-YOSHIDA; Tatsuhiko GOTO; Atsushi TOYODA; Katsunari IPPOUSHI; Mari MAEDA-YAMAMOTO; Yoshiharu TAKAYAMA; Chise SUZUKI, BMFH Press
Bioscience of Microbiota, Food and Health, 2024年03月, ［査読有り］

Metabolic profiles of saliva in male mouse models of chronic sleep disorders induced by psychophysiological stress.
Katsutaka Oishi; Yuhei Yajima; Yuta Yoshida; Hideo Hagihara; Tsuyoshi Miyakawa; Sayaka Higo-Yamamoto; Atsushi Toyoda, ラスト(シニア)オーサー, Disordered sleep is a global social problem and an established significant risk factor for psychological and metabolic diseases. We profiled non-targeted metabolites in saliva from mouse models of chronic sleep disorder (CSD). We identified 288 and 55 metabolites using CE-FTMS and LC-TOFMS, respectively, among which concentrations of 58 (CE-FTMS) and three (LC-TOFMS) were significantly changed by CSD. Pathway analysis revealed that CSD significantly suppressed glycine, serine and threonine metabolism. Arginine and proline metabolic pathways were among those that were both upregulated and downregulated. Pathways of alanine, aspartate and glutamate metabolism, genetic information processing, and the TCA cycle tended to be downregulated, whereas histidine metabolism tended to be upregulated in mice with CSD. Pyruvate, lactate, malate, succinate and the glycemic amino acids alanine, glycine, methionine, proline, and threonine were significantly decreased, whereas 3-hydroxybutyric and 2-hydroxybutyric acids associated with ketosis were significantly increased, suggesting abnormal glucose metabolism in mice with CSD. Increases in the metabolites histamine and kynurenic acid that are associated with the central nervous system- and decreased glycine, might be associated with sleep dysregulation and impaired cognitive dysfunction in mice with CSD. Our findings suggested that profiling salivary metabolites could be a useful strategy for diagnosing CSD.
Scientific reports, 2023年07月10日, ［査読有り］

Impacts of Subchronic and Mild Social Defeat Stress on Plasma Putrefactive Metabolites and Cardiovascular Structure in Male Mice.
Atsushi Toyoda; Kina Kawakami; Yuto Amano; Hideaki Nishizawa; Shin-Ichi Nakamura; Takahiro Kawase; Yuta Yoshida; Hodaka Suzuki; Takamitsu Tsukahara, 筆頭著者, Psychosocial stress precipitates mental illnesses, such as depression, and increases the risk of other health problems, including cardiovascular diseases. In this study, we observed the effects of psychosocial stress on the histopathological features of systemic organs and tissues in a mouse psychosocial stress model, namely the subchronic and mild social defeat stress (sCSDS) model. There were several pathological findings in the tissues of both sCSDS and control mice. Mild fibrosis of the heart was observed in sCSDS mice but not in control mice. Extramedullary hematopoiesis in the spleen and hemorrhage in the lungs were observed in both the control and sCSDS mice. Focal necrosis of the liver was seen only in control mice. Furthermore, putrefactive substances in the blood plasma were analyzed because these metabolites originating from intestinal fermentation might be linked to heart fibrosis. Among them, plasma p-cresyl glucuronide and p-cresyl sulfate concentrations significantly increased owing to subchronic social defeat stress, which might influence cardiac fibrosis in sCSDS mice. In conclusion, several pathological features such as increased cardiac fibrosis and elevated plasma putrefactive substances were found in sCSDS mice. Thus, sCSDS mice are a potential model for elucidating the pathophysiology of psychosocial stress and heart failure.
International journal of molecular sciences, 2023年01月08日, ［査読有り］

園芸と家畜との相互作用を含む農園活動が精神疾患の ある成人の心理的および生理的状態に及ぼす影響　　　　　　　　　　　　　　　
安江 健; 坂本希美; 平山 望; 小松﨑将一; 山川百合子; 高橋弘美; 柴沼沙織; 小針大助; 岡山 毅; 豊田 淳, ラスト(シニア)オーサー
ヒトと動物の関係学会誌, 2023年01月, ［査読有り］

Identification of salivary microRNA profiles in male mouse model of chronic sleep disorder.
Yuta Yoshida; Yuhei Yajima; Yuri Fujikura; Haotong Zhuang; Sayaka Higo-Yamamoto; Atsushi Toyoda; Katsutaka Oishi, Chronic sleep disorders (CSD) comprise a potential risk factor for metabolic and cardiovascular diseases, obesity and stroke. Thus, the identification of biomarkers for CSD is an important step in the early prevention of metabolic dysfunctions induced by sleep dysfunction. Diagnostic saliva samples can be easily and noninvasively collected. Thus, we aimed to identify whole microRNA (miRNA) profiles of saliva in control and psychophysiologically stressed CSD mouse models and compare them at Zeitgeber time (ZT) 0 (lights on) and ZT12 (lights off). The findings of two-way ANOVA revealed that the expression of 342 and 109 salivary miRNAs was affected by CSD and the time of day, respectively. Interactions were found in 122 miRNAs among which, we identified 197 (ZT0) and 62 (ZT12) upregulated, and 40 (ZT0) and seven (ZT12) downregulated miRNAs in CSD mice. We showed that miR-30c-5p, which is elevated in the plasma of patients with hypersomnia, was upregulated in the saliva of CSD mice collected at ZT0. The miRNAs, miR-10a-5p, miR-146b-5p, miR-150-5p, and miR-25-3p are upregulated in the serum of humans with poor sleep quality, and these were also upregulated in the saliva of CSD mice collected at ZT0. The miRNAs miR-30c, miR146b-5p, miR150, and miR-25-5p are associated with cardiovascular diseases, and we found that plasma concentrations of brain natriuretic peptides were significantly increased in CSD mice. The present findings showed that salivary miRNA profiles could serve as useful biomarkers for predicting CSD.
Stress (Amsterdam, Netherlands), 2023年01月, ［査読有り］

Salivary microRNA and Metabolic Profiles in a Mouse Model of Subchronic and Mild Social Defeat Stress.
Yuta Yoshida; Yuhei Yajima; Kina Kawakami; Shin-Ichi Nakamura; Takamitsu Tsukahara; Katsutaka Oishi; Atsushi Toyoda, ラスト(シニア)オーサー, Identification of early biomarkers of stress is important for preventing mood and anxiety disorders. Saliva is an easy-to-collect and non-invasive diagnostic target. The aim of this study was to characterize the changes in salivary whole microRNAs (miRNAs) and metabolites in mice subjected to subchronic and mild social defeat stress (sCSDS). In this study, we identified seven upregulated and one downregulated miRNAs/PIWI-interacting RNA (piRNA) in the saliva of sCSDS mice. One of them, miR-208b-3p, which is reported as a reliable marker for myocardial infarction, was upregulated in the saliva of sCSDS mice. Histological analysis showed frequent myocardial interstitial fibrosis in the heart of such mice. In addition, gene ontology and pathway analyses suggested that the pathways related to energy metabolism, such as the oxidative phosphorylation and the pentose phosphate pathway, were significantly related to the miRNAs affected by sCSDS in saliva. In contrast, salivary metabolites were not significantly changed in the sCSDS mice, which is consistent with our previous metabolomic study on the plasma of sCSDS mice. Taken in the light of previous studies, the present study provides novel potential stress biomarkers for future diagnosis using saliva.
International journal of molecular sciences, 2022年11月21日, ［査読有り］

Dietary Hesperidin Suppresses Lipopolysaccharide-Induced Inflammation in Male Mice.
Mizuho Sato; Alato Okuno; Keita Ishisono; Yuhei Yajima; Atsushi Toyoda, ラスト(シニア)オーサー, Depressive disorders are partially attributed to chronic inflammation associated with the tryptophan (Trp)-kynurenine (Kyn) pathway. Recent evidence suggests that anti-inflammatory agents may reduce the risk of depression. The present study aimed to elucidate the potential of the citrus flavonoid hesperidin, which exhibits anti-inflammatory activity, in suppressing the Trp-Kyn pathway in the brain, using a lipopolysaccharide (LPS)-induced inflammation mouse model. Dietary hesperidin was found to suppress activation of the Trp-Kyn pathway in the prefrontal cortex. In addition, it reduced systemic LPS-induced signs of illness, such as low skin temperature and enhanced leukocyte count in the blood. However, dietary supplementation with hesperidin did not improve body weight loss, food intake, water intake, or splenic increases in leukocyte numbers in the LPS model. Collectively, the results suggest that dietary hesperidin can partially regulate central and peripheral events linked to inflammation in LPS mouse models.
International journal of tryptophan research : IJTR, 2022年10月, ［査読有り］

Effect of Probiotic Bifidobacterium bifidum TMC3115 Supplementation on Psychosocial Stress Using a Sub-Chronic and Mild Social Defeat Stress in Mice.
Kazutoyo Yoda; Gaku Harata; Mizuho Sato; Kenji Miyazawa; Natsuki Ohsawa; Fang He; Atsushi Toyoda, ラスト(シニア)オーサー, With the accumulation of knowledge on the relation between psychological stress and gut microbiota, there is growing interest in controlling stress and/or mood disorders via probiotic supplementation. We aimed to examine the effect of probiotic Bifidobacterium bifidum TMC3115 (TMC3115) supplementation using a sub-chronic and mild social defeat stress murine model in this study. TM3115 supplementation maintained body weight gain and alleviated a polydipsia-like symptom induced by the stress. In the analyses of fecal and cecal bacterial profiles, expansions of Proteobacteria in stressed mice and increases in Actinobacteria and Bifidobacterium in mice supplemented with TMC3115 were observed. There was no marked difference in the diversity of cecal bacteria between the tested mice. Elevated serum levels of inflammatory markers such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 were observed in the stressed mice, while TMC3115 only reduced the IL-6 level. These findings suggest that TMC3115 supplementation confers tolerance to psychosocial stress in the host through modulation of the gut microbiota and alleviation of stress-induced inflammatory responses. Furthermore, it may be expected to exert prevention and treatment of disorders related to peripheral IL-6, including depression.
Nutrients, 2022年02月24日, ［査読有り］

Differential Effect of Non-Purified and Semi-Purified Standard Diets on Kynurenine and Peripheral Metabolites in Male C57BL/6J Mice.
Yuhei Yajima; Alato Okuno; Isamu Nakamura; Teruo Miyazaki; Akira Honda; Atsushi Toyoda, ラスト(シニア)オーサー, The kynurenine (Kyn) pathway plays crucial roles in several inflammation-induced disorders such as depression. In this study, we measured Kyn and other related molecules in the blood plasma, brain, and urine of male C57BL/6J mice (B6) fed non-purified (MF) and semi-purified (AIN-93G and AIN-93M) standard rodent diets. Mice fed MF had increased plasma Kyn levels compared with those on AIN93-based diets, as well as decreased hippocampal Kyn levels compared with those fed AIN-93G. Previous studies showed that branched chain amino acids (BCAAs) suppress peripheral blood Kyn transportation to the brain, but plasma BCAA levels were not significantly different between the diet groups in our study. Urine metabolome analysis revealed that feed ingredients affected the excretion of many metabolites, and MF-fed mice had elevated excretion of kynurenic and quinolinic acids, pivotal metabolites in the Kyn pathway. Collectively, the level of critical metabolites in the Kyn pathway in the central and peripheral tissues was strongly affected by feed ingredients. Therefore, feed selection is a critical factor to ensure the reproducibility of experimental data in studies involving rodent models.
International journal of tryptophan research : IJTR, 2022年, ［査読有り］

Estimating body weight of pigs from posture analysis using a depth camera.
Tsuyoshi Okayama; Yoshifumi Kubota; Atsushi Toyoda; Daisuke Kohari; Go Noguchi, A noninvasive method for estimating the body weight (BW) of a pig considering its posture using a low-cost depth camera (Kinect v2) was proposed. A total of 150 pigs were used, and 738 depth images (point clouds) were obtained for them. The pig "volume" was calculated from the pig point cloud, and it was found to have a very high correlation to BW. To evaluate the posture of a pig quantitatively, seven posture angles were calculated based on the "spine" extracted from a pig point cloud. We found the posture angles representing the height of the head position correlated with the accuracy of BW estimation using the "volume." Based on this finding, we proposed an "adjusted volume," which was adjusted based on the relationship between the posture angles and the estimation error. The BW of pigs was estimated using the simple regression model with the "adjusted volume," and the MAPE and RMSPE were 4.87% and 6.13%, respectively. The accuracy of the suggested model was similar to that of the volume-based estimation models of other studies that used only data with an appropriate pig posture for BW estimation.
Animal science journal = Nihon chikusan Gakkaiho, 2021年12月, ［査読有り］

Protein lactylation induced by neural excitation.
Hideo Hagihara; Hirotaka Shoji; Hikari Otabi; Atsushi Toyoda; Kaoru Katoh; Masakazu Namihira; Tsuyoshi Miyakawa, Lactate has diverse roles in the brain at the molecular and behavioral levels under physiological and pathophysiological conditions. This study investigates whether lysine lactylation (Kla), a lactate-derived post-translational modification in macrophages, occurs in brain cells and if it does, whether Kla is induced by the stimuli that accompany changes in lactate levels. Here, we show that Kla in brain cells is regulated by neural excitation and social stress, with parallel changes in lactate levels. These stimuli increase Kla, which is associated with the expression of the neuronal activity marker c-Fos, as well as with decreased social behavior and increased anxiety-like behavior in the stress model. In addition, we identify 63 candidate lysine-lactylated proteins and find that stress preferentially increases histone H1 Kla. This study may open an avenue for the exploration of a role of neuronal activity-induced lactate mediated by protein lactylation in the brain.
Cell reports, 2021年10月12日, ［査読有り］

Subchronic and mild social defeat stress downregulates peripheral expression of sweet and umami taste receptors in male mice.
Yuta Yoshida; Misa Miyazaki; Yuhei Yajima; Atsushi Toyoda, ラスト(シニア)オーサー, Depression is associated with taste disorders; however, the mechanisms by which mental stress affects taste perception are not well understood. This study aimed to elucidate the effects of psychosocial stress on peripheral taste-sensing systems using a mouse depression model. Male mice were subjected to subchronic and mild social defeat stress (sCSDS). Results showed that sCSDS significantly increased body weight, food and water intake, and social avoidance behavior and that sCSDS did not change reward-seeking behavior on sucrose preference but tended to decrease pheromonal preference for female urine. Furthermore, sCSDS downregulated the mRNA levels of sweet and umami taste receptor subunits, i.e., sweet taste receptor type 1 members 2 and 3 (T1R2 and T1R3), but not the umami taste receptor subunit, i.e., taste receptor type 1 member 1 (T1R1), in the circumvallate papillae of mice. It is known that sucrose preference is mediated by the gut-brain axis without taste perception; thus, it was considered that sCSDS affected the peripheral taste-sensing systems, rather than the central reward systems, which mediate sucrose preference. This is the first study to report that psychosocial stress affects peripheral sweet and umami taste-sensing systems.
Biochemical and biophysical research communications, 2021年09月24日, ［査読有り］

Metabolic and Microbial Characterizations for the Gastrointestinal Digesta of the Zoo Colobus guereza
Toyoda A; Shionome N; Kohari D; Iida S; Masato H; Namae N; Nakamura S; Tsukahara T, 筆頭著者, Austin publishing
Journal of Bacteriology and Mycology, 2021年02月12日, ［査読有り］

The effects of chronic social defeat stress on solid gastric epmtying and defecation in C57BL/6J mice
Naoko Moriya; Naoko Yamagishi; Tatsuhiko Goto; Hikari Otabi; Hiromi Nira-Kimoto; Atsushi Toyoda; Chise Suzuki, Japan International Research Center for Agricultural Sciences
Japan Agriculture Research Quarterly, 2021年01月, ［査読有り］

Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations.
Takamitsu Tsukahara; Atsushi Toyoda; Takahiro Kawase; Shin-Ichi Nakamura; Kuniyasu Ochiai, BACKGROUND: Periodontopathic bacteria such as Porphyromonas gingivalis produce several metabolites, including lipopolysaccharide (LPS) and n-butyric acid (BA). Past work suggested that periodontal infection may cause cognitive impairment in mice. AIMS: To elucidate the mechanisms by which metabolites such as LPS and BA, resulting from Porphyromonas gingivalis activity, induce immunological and physiological abnormalities in mice. METHODS: In the present work, 28 male ICR mice were placed in an open-field arena and the total distance (cm/600 s) they covered was recorded. Based on their moving distances, mice were divided into 4 groups (n = 7) and injected the following substances into their gingival tissues for 32 consecutive days: saline (C), 5 mmol/L of BA (B), 1 μg/mouse of LPS (L), and BA-LPS (BL) solutions. Distances covered by mice were also measured on days 14 and 21, with their habituation scores considered as "(moving distance on day 14 or 21)/(moving distance on day 0)". Afterwards, mice were dissected, and hippocampal gene expression and the concentrations of short-chain fatty acids, neurotransmitters and cytokines in their blood plasma and brains were analyzed. In addition, mouse brain and liver tissues were fixed and visually assessed for histopathological abnormalities. RESULTS: Group BL had significantly higher habituation scores than C and B on day 14. LPS induced higher habituation scores on day 21. LPS induced significant decreases in the mRNA levels of interleukin (IL)-6 and brain-derived neurotrophic factors, and an increase in neurotrophic tyrosine kinase receptor type 2. In both plasma and brain, LPS induced a significant acetate increase. Moreover, LPS significantly increased acetylcholine in brain. In plasma alone, LPS and BA significantly decreased monocyte chemoattractant protein 1 (MCP-1). However, while LPS significantly decreased tyrosine, BA significantly increased it. Lastly, LPS significantly decreased IL-6 and tumor necrosis factor in plasma. No histopathological abnormalities were detected in liver or brain tissues of mice. CONCLUSION: We showed that injections of LPS and/or BA induced mice to move seemingly tireless and that both LPS and BA injections strongly induced a reduction of MCP-1 in blood plasma. We concluded that LPS and BA may have been crucial to induce and/or aggravate abnormal behavior in mice.
Journal of neuroinflammation, 2020年11月05日, ［査読有り］

Reduction in BDNF from Inefficient Precursor Conversion Influences Nest Building and Promotes Depressive-Like Behavior in Mice.
Masami Kojima; Hikari Otabi; Haruko Kumanogoh; Atsushi Toyoda; Masahito Ikawa; Masaru Okabe; Toshiyuki Mizui, We generated a knock-in mouse line in which the gene encoding brain-derived neurotrophic factor (Bdnf) was replaced with a sequence for proBDNF containing human single nucleotide polymorphisms encoding arginines proximal to the cleavage site (R125M and R127L). The ratio of the mature form of BDNF (mBDNF) to precursor BDNF (proBDNF) in hippocampal tissue lysates was decreased in a manner dependent on the number of copies of the mutant gene, indicating that the mutations inhibited proteolytic conversion of proBDNF into mBDNF. Although homozygous mice had a proBDNF/mBDNF ratio of ~9:1, they survived until adulthood. The levels of mBDNF were reduced by 57% in heterozygous mutant mice, which exhibited a depressive-like behavior in the tail suspension test and weight gain when housed in social isolation, showing that impaired proBDNF cleavage contributes to stress-induced depressive-like phenotypes. Furthermore, socially isolated heterozygous mice displayed a pronounced deficit in daily nest-building behaviors. These findings suggest that the decreased production of mBDNF by impaired proBDNF cleavage disturbs daily activities in mice.
International journal of molecular sciences, 2020年06月01日, ［査読有り］

Impaired bile acid metabolism with defectives of mitochondrial-tRNA taurine modification and bile acid taurine conjugation in the taurine depleted cats.
Teruo Miyazaki; Sei-Ich Sasaki; Atsushi Toyoda; Fan-Yan Wei; Mutsumi Shirai; Yukio Morishita; Tadashi Ikegami; Kazuhito Tomizawa; Akira Honda, Taurine that conjugates with bile acid (BA) and mitochondrial-tRNA (mt-tRNA) is a conditional essential amino acid in humans, similarly to cats. To better understand the influence of acquired depletion of taurine on BA metabolism, the profiling of BAs and its intermediates, BA metabolism-enzyme expression, and taurine modified mt-tRNAs were evaluated in the taurine deficient diet-supplemented cats. In the taurine depleted cats, taurine-conjugated bile acids in bile and taurine-modified mt-tRNA in liver were significantly decreased, whereas unconjugated BA in serum was markedly increased. Impaired bile acid metabolism in the liver was induced accompanied with the decreases of mitochondrial cholesterol 27-hydroxylase expression and mitochondrial activity. Consequently, total bile acid concentration in bile was significantly decreased by the low activity of mitochondrial bile acid synthesis. These results implied that the insufficient dietary taurine intake causes impaired bile acid metabolism, and in turn, a risk for the various diseases similar to the mitochondrial diseases would be enhanced.
Scientific reports, 2020年03月18日, ［査読有り］

Coenzyme A and Its Thioester Pools in Obese Zucker and Zucker Diabetic Fatty Rats.
Shigeru Chohnan; Shiori Matsuno; Kei Shimizu; Yuka Tokutake; Daisuke Kohari; Atsushi Toyoda, ラスト(シニア)オーサー, Feeding behavior is closely related to hypothalamic malonyl-CoA level in the brain and diet-induced obesity affects total CoA pools in liver. Herein, we performed a comprehensive analysis of the CoA pools formed in thirteen tissues of Zucker and Zucker diabetic fatty (ZDF) rats. Hypothalamic malonyl-CoA levels in obese rats remained low and were almost the same as those of lean rats, despite obese rats having much higher content of leptin, insulin, and glucose in their sera. Regardless of the fa-genotypes, larger total CoA pools were formed in the livers of ZDF rats and the size of hepatic total CoA pools in Zucker rats showed almost one tenth of the size of ZDF rats. The decreased total CoA pool sizes in Zucker rats was observed in the brown adipose tissues, while ZDF-fatty rats possessed 6% of total CoA pool in the lean rats in response to fa deficiency. This substantially lower CoA content in the obese rats would be disadvantageous to non-shivering thermogenesis. Thus, comparing the intracellular CoA behaviors between Zucker and ZDF rats, as well as the lean and fatty rats of each strain would help to elucidate features of obesity and type 2 diabetes in combination with result (s) of differential gene expression analysis and/or comparative genomics.
Nutrients, 2020年02月06日, ［査読有り］

Metabolomic analyses of plasma and liver of mice fed with immature Citrus tumida peel.
Atsushi Toyoda; Mizuho Sato; Masaki Muto; Tatsuhiko Goto; Yuji Miyaguchi; Eiichi Inoue, 筆頭著者, In this study, we investigated the effects of dietary supplementation of Citrus tumida hort. ex Tanaka on food intake, body and fat tissue weights, and metabolic profiles of plasma and liver in mice. Supplementation with 5% (w/w) of peels of immature C. tumida (PIC) for 4 weeks significantly suppressed body weight gain and decreased adipose tissue weight in epididymal, perirenal, and subcutaneous fats. Metabolome analyses showed that 2-hydroxyvaleric acid levels were reduced in the blood plasma of mice fed with PIC. PIC supplementation significantly elevated dipeptide (Thr-Asp, Ser-Glu, and Ala-Ala), glucuronic acid, and S-methylglutathione levels, and significantly reduced betaine aldehyde levels in the liver. In conclusion, PIC supplementation affects the metabolism of fatty acids, pectin, glutathione, and choline, showing potential beneficial effects for metabolic syndrome and obesity. PIC may be developed as a functional food and used in the treatment of these diseases.
Bioscience, biotechnology, and biochemistry, 2020年02月04日, ［査読有り］

Assessment of nest building and social interaction behavior in mice exposed to acute social defeat stress using a three-dimensional depth camera.
Hikari Otabi; Tsuyoshi Okayama; Atsushi Toyoda, ラスト(シニア)オーサー, Nest building is an instinctive behavior toward protection from predators, body temperature regulation, and courtship. Previously, we discovered that acute and chronic social defeat stress suppresses the onset of nest-building behavior in male mice (C57BL/6J). Here, we analyzed nest building and other behavioral deficits induced by acute social defeat stress (ASDS). We utilized a customized cage and specifically developed observational programs for nest building, social avoidance, and other behaviors using an infrared depth camera to acquire three-dimensional (3D) data of animal behavior (Negura system). We determined the volume of nesting materials from these 3D depth images. Mice exposed to ASDS showed increased spontaneous activities, decreased rearing, and delayed nest building; however, nest-building activity was gradually recovered during the dark period of the 24 hr observation interval. At the endpoint following 24 hr, the ASDS and control groups showed no differences in nest volumes. Furthermore, we observed the time courses of both nest building and social avoidance behaviors and their relationship using the Negura system. Our data demonstrated a weak positive correlation between nest-building delay and social avoidance in ASDS mice. The Negura system can observe various behaviors that reflect the effects of social defeat stress.
Animal science journal = Nihon chikusan Gakkaiho, 2020年01月, ［査読有り］

Effects of dietary intake of heat-inactivated Lactobacillus gasseri CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model.
Atsushi Toyoda; Takahiro Kawase; Takamitsu Tsukahara, 筆頭著者, The intestinal ecosystem is involved in the pathogenesis of mood disorders such as depression. Intestinal microbes can affect the central nervous system through the gut-brain axis, which raises the possibility of using probiotics for preventing depression. In this study, we examined the effect of heat-inactivated Lactobacillus gasseri CP2305 (CP2305) in a subchronic and mild social defeat stress (sCSDS) mouse model. sCSDS suppressed food intake. However, dietary CP2305 intake rescued it, suggesting that CP2305 improved the decreased appetite in sCSDS mice. sCSDS did not alter the gene expression of brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 in the hippocampus. However, dietary CP2305 provided following sCSDS increased the gene expression of these neurotrophins in the hippocampus. These findings suggest that CP2305 supplementation would aid in preventing psychosocial stress-induced disorders.
Biomedical research (Tokyo, Japan), 2020年, ［査読有り］

Preliminary investigation of the effect of oral supplementation of Lactobacillus plantarum strain SNK12 on mRNA levels of neurotrophic factors and GABA receptors in the hippocampus of mice under stress-free and sub-chronic mild social defeat-stressing con
Tsukahara T; Kawase T; Yoshida H; Bukawa W; Kan T; Toyoda A, ラスト(シニア)オーサー
Bioscience, biotechnology, and biochemistry, 2019年09月, ［査読有り］

Dietary intake of the citrus flavonoid hesperidin affects stress-resilience and brain kynurenine levels in a subchronic and mild social defeat stress model in mice.
Sato M; Okuno A; Suzuki K; Ohsawa N; Inoue E; Miyaguchi Y; Toyoda A, ラスト(シニア)オーサー, Depressive disorders are partly caused by chronic inflammation through the kynurenine (KYN) pathway. Preventive intervention using anti-inflammatory reagents may be beneficial for alleviating the risk of depression. In this study, we focused on the Japanese local citrus plant, Citrus tumida hort. ex Tanaka (C. tumida; CT), which contains flavonoids such as hesperidin that have anti-inflammatory actions. The dietary intake of 5% immature peels of CT fruits slightly increased stress resilience in a subchronic and mild social defeat (sCSDS) model in mice. Moreover, the dietary intake of 0.1% hesperidin significantly increased stress resilience and suppressed KYN levels in the hippocampus and prefrontal cortex in these mice. In addition, KYN levels in the hippocampus and prefrontal cortex were significantly correlated with the susceptibility to stress. In conclusion, these results suggest that dietary hesperidin increases stress resilience by suppressing the augmentation of KYN signaling under sCSDS.
Bioscience, biotechnology, and biochemistry, 2019年05月, ［査読有り］

Heat-killed Lactobacillus helveticus strain MCC1848 confers resilience to anxiety or depression-like symptoms caused by subchronic social defeat stress in mice.
Maehata H; Kobayashi Y; Mitsuyama E; Kawase T; Kuhara T; Xiao JZ; Tsukahara T; Toyoda A, ラスト(シニア)オーサー
Bioscience, biotechnology, and biochemistry, 2019年03月, ［査読有り］

Comparison of gut tight junction gene expression in C57BL/6J and BALB/c mice after chronic social defeat stress.
Yamagishi N; Omata Y; Aoki-Yoshida A; Moriya N; Goto T; Toyoda A; Aoki R; Suzuki C; Takayama Y
Japan Agricultural Research Quartely, 2019年01月, ［査読有り］

Dietary Intake of Immature Citrus tumida Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity.
Sato M; Goto T; Inoue E; Miyaguchi Y; Toyoda A, ラスト(シニア)オーサー
Journal of nutritional science and vitaminology, 2019年, ［査読有り］

ワイヤレス 3 軸加速度計による精神障がい者と健常者の園芸作業の動作比較　　　　　　　　　　　　　　　
ハニン サニ デビ; 小松﨑将一; 山川百合子; 高橋弘美; 柴沼沙織; 安江健; 岡山毅; 豊田淳, ラスト(シニア)オーサー
農作業研究, 2018年12月, ［査読有り］

Effects of housing conditions on behaviors and biochemical parameters in juvenile cynomolgus monkeys (Macaca fascicularis).
Koyama H; Tachibana Y; Takaura K; Takemoto S; Morii K; Wada S; Kaneko H; Kimura M; Toyoda A, ラスト(シニア)オーサー
Experimental animals, 2018年12月, ［査読有り］

Reduced fucosylation in the distal intestinal epithelium of mice subjected to chronic social defeat stress.
Yasuhiro Omata; Reiji Aoki; Ayako Aoki-Yoshida; Keiko Hiemori; Atsushi Toyoda; Hiroaki Tateno; Chise Suzuki; Yoshiharu Takayama, Psychological stress can cause dysfunction of the gastrointestinal tract by regulating its interaction with central nervous system (brain-gut axis). Chronic social defeat stress (CSDS) is widely used to produce a rodent model of stress-induced human mood disorders and depression. We previously showed that CSDS significantly affects the intestinal ecosystem including cecal and fecal microbiota, intestinal gene expression profiles and cecal metabolite profiles. Here, we investigated whether the glycosylation pattern in the intestinal epithelium was affected in C57BL/6 mice exposed to CSDS (hereinafter referred to as CSDS mice). A lectin microarray analysis revealed that CSDS significantly reduced the reactivity of fucose-specific lectins (rAOL, TJA-II, rAAL, rGC2, AOL, AAL, rPAIIL and rRSIIL) with distal intestinal mucosa, but not with mucosa from proximal intestine and colon. Flow cytometric analysis confirmed the reduced TJA-II reactivity with intestinal epithelial cells in CSDS mice. In addition, distal intestine expression levels of the genes encoding fucosyltransferase 1 and 2 (Fut1 and Fut2) were downregulated in CSDS mice. These findings suggest that CSDS alters the fucosylation pattern in the distal intestinal epithelium, which could be used as a sensitive marker for CSDS exposure.
Scientific reports, 2018年09月04日, ［査読有り］

Development of Implantable Wireless Sensor Nodes for Animal Husbandry and MedTech Innovation.
Jian Lu; Lan Zhang; Dapeng Zhang; Sohei Matsumoto; Hiroshi Hiroshima; Ryutaro Maeda; Mizuho Sato; Atsushi Toyoda; Takafumi Gotoh; Nobuhiro Ohkohchi, In this paper, we report the development, evaluation, and application of ultra-small low-power wireless sensor nodes for advancing animal husbandry, as well as for innovation of medical technologies. A radio frequency identification (RFID) chip with hybrid interface and neglectable power consumption was introduced to enable switching of ON/OFF and measurement mode after implantation. A wireless power transmission system with a maximum efficiency of 70% and an access distance of up to 5 cm was developed to allow the sensor node to survive for a duration of several weeks from a few minutes' remote charge. The results of field tests using laboratory mice and a cow indicated the high accuracy of the collected biological data and bio-compatibility of the package. As a result of extensive application of the above technologies, a fully solid wireless pH sensor and a surgical navigation system using artificial magnetic field and a 3D MEMS magnetic sensor are introduced in this paper, and the preliminary experimental results are presented and discussed.
Sensors (Basel, Switzerland), 2018年03月26日, ［査読有り］

Effects of non-purified and semi-purified commercial diets on behaviors, plasma corticosterone levels, and cecum microbiome in C57BL/6J mice
Atsushi Toyoda; Hikari Shimonishi; Mizuho Sato; Kento Usuda; Natsuki Ohsawa; Kentaro Nagaoka, 筆頭著者, Diverse commercially available feeds are used in animal studies according to the purpose of the studies. We sought to understand the relationship between feed ingredients and their effects on animal physiology and behaviors. Here, we investigated how male laboratory mice (C57BL/6J (“B6”) mice) were affected by chronic feeding with two commercially available diets, a non-purified diet (MF) and a semi-purified diet (AIN-93G). In B6 mice, both diets similarly induced spontaneous activities in the home cage and the open field box, anxiety in the elevated plus maze test, and depressive-like behaviors in tail-suspension and forced-swimming tests, and with both diets, similar data were obtained on calorie intake, water intake, body weight gain, and plasma corticosterone levels. By contrast, liver weight was significantly higher in MF-fed B6 mice than in AIN-93G-fed B6 mice. Furthermore, the cecum microbiome was drastically affected by the diets, and, specifically, Allobaculum was the major genus (43.4%) in the cecum microbiota of AIN-93G-fed mice but its abundance was reduced (to 3.8%) in the case of MF-fed mice. Future studies should address whether the differences in diet purity and cecum microbiota influence brain functions and behaviors in B6 mice., Elsevier Ireland Ltd
Neuroscience Letters, 2018年03月23日, ［査読有り］

Effects of Diet Quality and Psychosocial Stress on the Metabolic Profiles of Mice
Tatsuhiko Goto; Shozo Tomonaga; Atsushi Toyoda, ラスト(シニア)オーサー, There has been an increasing interest in relationship between stress and diet. To address this relationship, we evaluated an animal model of depression: male C57BL/6J mice subjected to subchronic mild social defeat stress (sCSDS) for 10 consecutive days using male ICR mice under two different calorie-adjusted diets conditions-nonpurified (MF) and semi purified (AIN) diets made from natural and chemical ingredients mainly, respectively. Our previous study indicates that diet quality and purity affect stress susceptibility in sCSDS mice. We therefore hypothesized that there are some key peripheral metabolites to change stress-susceptible behavior. GC-MS metabolomics of plasma, liver, and cecal content were performed on four test groups: sCSDS + AIN diet (n = 7), sCSDS + MF diet (n = 6), control (no sCSDS) + AIN diet (n = 8), and control + MF diet (n = 8). Metabolome analyses revealed that the number of metabolites changed by food was larger than the number changed by stress in all tissues. Enrichment analysis of the liver stress-susceptible mice show increased glycolysis-related substrates in the liver. We found metabolites that were affected by stress (e.g., plasma and liver 4-hydroxyproline and plasma beta-alanine are higher in sCSDS than in control) and a stress x food interaction (e.g., plasma GABA is lower in sCSDS + AIN than in sCSDS + MF). Because functional compounds were altered by both stress and food, diet may be able to attenuate various stress-induced symptoms by changing metabolites in peripheral tissues., AMER CHEMICAL SOC
JOURNAL OF PROTEOME RESEARCH, 2017年05月, ［査読有り］

The acute social defeat stress and nest-building test paradigm: A potential new method to screen drugs for depressive-like symptoms
Hikari Otabi; Tatsuhiko Goto; Tsuyoshi Okayama; Daisuke Kohari; Atsushi Toyoda, Psychosocial stress can cause mental conditions such as depression in humans. To develop drug therapies for the treatment of depression, it is necessary to use animal models of depression to screen drug candidates that exhibit anti-depressive effects. Unfortunately, the present methods of drug screening for antidepressants, the forced-swim test and tail-suspension test, are limiting factors in drug discovery because they are not based on the constructive validity of objective phenotypes in depression. Previously, we discovered that the onset of nest building is severely delayed in mice exposed to subchronic mild social defeat stress (sCSDS). Therefore, a novel paradigm combining acute social defeat stress (ASDS) and the nest-building test (SNB) were established for the efficient screening of drugs for depressive-like symptoms. Since ASDS severely delayed the nest-building process as shown in chronically social defeated mice, we sought to rescue the delayed nest-building behavior in ASDS mice. Injecting a specific serotonin 2a receptor antagonist (SR-46349B), the nest-building deficit exhibited by ASDS mice was partially rescued. On the other hand, a selective serotonin reuptake inhibitor (fluoxetine) did not rescue the nest building deficit in ASDS mice. Therefore, we conclude that the SNB paradigm is an another potential behavioral method for screening drugs for depressive-like symptoms including attention deficit, anxiety, low locomotion, and decreased motivation. (C) 2016 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
BEHAVIOURAL PROCESSES, 2017年02月, ［査読有り］

Effects of combined treatment with fermented soybean (natto) intake and exercise on bone metabolism in ovariectomized rats.
Kawano M; Itomine I; Monma M; Asakawa K; Toyoda A; Miyaguchi Y, OBJECTIVES: Using ovariectomized rats, we examined the influence of combined exercise tolerance and natto intake on the bone loss inhibitory effect. METHODS: We divided female Wistar rats into the following groups: Ovariectomy, Ovariectomy + Exercise, Ovariectomy + Natto Intake, Ovariectomy + Exercise + Natto Intake, and Pseudo-operative (Sham group). After conducting experiments on each group, we collected the tissues and performed morphological and molecular biological analyses. RESULTS: In comparison with the Ovariectomy group, only in the Ovariectomy + Exercise group was there a significant bone loss inhibitory effect in the femoral cancellous bone. Although there was a tendency toward this trend seen in the Natto Intake and Exercise + Natto Intake groups, these differences were not significant. The increase in messenger RNA expression levels of alkaline phosphatase (osteoblast marker) in the bone marrow caused by ovariectomy was suppressed by individual factors, and by those in combination. However, messenger RNA expression levels of estrogen receptor alpha in the bone marrow showed a decreasing tendency with each factor, and decreased significantly with the combination, similar to the Sham group. CONCLUSION: This suggests that natto intake and exercise maintain bone mass by different molecular mechanisms and that these two factors do not simply act synergistically in combination to maintain bone mass.
SAGE open medicine, 2017年, ［査読有り］

Community Gardens as Health Promoters: Effects on Mental and Physical Stress Levels in Adults with and without Mental Disabilities
Nugrahaning Sani Dewi; Masakazu Komatsuzaki; Yuriko Yamakawa; Hiromi Takahashi; Saori Shibanuma; Takeshi Yasue; Tsuyoshi Okayama; Atsushi Toyoda; Hikari Shimonishi; Seiichi Sasaki, The study focuses on psychological and physical effects of stress while performing community garden activities of various intensity levels. The aim of this study was to determine the psychological and physical effects in adults with (case group) and without (control group) mental disabilities. Salivary alpha-amylase (sAA) levels and the stress response scale (SRS-18) were used for the psychological analysis (n = 42). For physical assessment (n = 13), electrocardiogram (ECG), surface electromyogram (sEMG), and respiration rate were continuously measured while performing the activities using a multichannel telemetry system. The results showed that following the activities, the case group exhibited decreasing sAA levels while control group exhibited increasing sAA levels. However, both groups exhibited lower SRS-18 results following the activities. Compared with the control group, the case group had a significantly lower increase in the ratio of the heart rate (IRHR) (5.5%) during low-intensity work (filling pots with soil), but a significantly higher IRHR (16.7%) during high-intensity work (turning over soil). The case group experienced significantly higher levels of fatigue during high-intensity work (digging) than during the rest condition. These findings indicate that appropriate workload allocation, according to health, is necessary in the community garden setting because reducing the intensity of work assignments for people with mental disabilities will reduce their physical stress., MDPI AG
SUSTAINABILITY, 2017年01月, ［査読有り］

Omics Studies of the Murine Intestinal Ecosystem Exposed to Subchronic and Mild Social Defeat Stress
Ayako Aoki-Yoshida; Reiji Aoki; Naoko Moriya; Tatsuhiko Goto; Yoshifumi Kubota; Atsushi Toyoda; Yoshiharu Takayama; Chise Suzuki, The microbiota-gut-brain axis plays an important role in the development of stress-induced mental disorders. We previously established the subchronic and mild social defeat stress (sCSDS) model, a murine experimental model of depression, and investigated the metabolomic profiles of plasma and liver. Here we used omits approaches to identify stress-induced changes in the gastrointestinal tract. Mice exposed to sCSDS for 10 days showed the following changes: (1) elevation of cholic acid and reduction of 5-aminovaleric acid among cecal metabolites; (2) downregulation of genes involved in the immune response in the terminal ileum; (3) a shift in the diversity of the microbiota in cecal contents and feces; and (4) fluctuations in the concentrations of cecal metabolites produced by gut microbiota reflected in plasma and hepatic metabolites. Operational taxonomic units within the family Lachnospiraceae showed an inverse correlation with certain metabolites. The social interaction score correlated with cecal metabolites, IgA, and cecal and fecal microbiota, suggesting that sCSDS suppressed the ileal immune response, altering the balance of microbiota, which together with host cells and host enzymes resulted in a pattern of accumulated metabolites in the intestinal ecosystem distinct from that of control mice., AMER CHEMICAL SOC
JOURNAL OF PROTEOME RESEARCH, 2016年09月, ［査読有り］

未熟フクレミカン果皮の給与がマウスの増体に及ぼす影響　　　　　　　　　　　　　　　
佐藤 瑞穂; 白取 知大; 後藤 達彦; 井上 栄一; 豊田 淳, (公社)日本栄養・食糧学会
日本栄養・食糧学会大会講演要旨集, 2016年04月

Decreased hepatic contents of coenzyme A molecular species in mice after subchronic mild social defeat stress
Yoshifumi Kubota; Tatsuhiko Goto; Yuki Hagiya; Shigeru Chohnan; Atsushi Toyoda, Social stress may precipitate psychiatric disorders such as depression, which is related to the occurrence of the metabolic syndrome, including obesity and type 2 diabetes. We have evaluated the effects of social stress on central and peripheral metabolism using a model of depression in mice. In the present study, we focused on coenzyme A (CoA) molecular species [i.e. non-esterified CoA (CoASH), acetyl-CoA and malonyl-CoA] which play important roles in numerous metabolic pathways, and we analyzed changes in expression of these molecules in the hypothalamus and liver of adult male mice (C57BL/6J) subjected to 10 days of subchronic mild social defeat stress (sCSDS) with ICR mice as aggressors. Mice (n=12) exposed to showed hyperphagia- and polydipsia-like symptoms and increased body weight gain compared with control mice which were not affected by exposure to ICR mice (n=12). To elucidate the underlying metabolic features in the sCSDS model, acetyl-CoA, malonyl-CoA and CoASH tissue levels were analyzed using the acyl-CoA cycling method. The levels of hypothalamic malonyl-CoA, which decreases feeding behavior, were not influenced by sCSDS. However, sCSDS reduced levels of acetyl-CoA, malonyl-CoA and total CoA (sum of the three CoA molecular species) in the liver. Hence, hyperphagia-like symptoms in sCSDS mice evidently occurred independently of hypothalamic malonyl-CoA, but might consequently lead to down-regulation of hepatic CoA via altered expression of nudix hydrolase 7. Future studies should investigate the molecular mechanism(s) underlying the down-regulation of liver CoA pools in sCSDS mice., TAYLOR & FRANCIS LTD
STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, 2016年03月, ［査読有り］

Effects of diet quality on vulnerability to mild subchronic social defeat stress in mice
Tatsuhiko Goto; Yoshifumi Kubota; Atsushi Toyoda, Objectives: The chronic social defeat stress (CSDS) mouse model is a potentially useful system for understanding stress responses to social environments. We previously developed a mouse model of subchronic and mild social defeat stress (sCSDS) that exhibits increased body weight gain and food intake following polydipsia-like features. sCSDS mice also show avoidance behavior in a social interaction test. In this study, we examined the effects of diet quality on susceptibility to sCSDS by feeding these mice semi-and non-purified diets.
Methods: Male C57BL/6J (B6; n = 82) mice were exposed to sCSDS using male ICR mice. The B6 mice were divided into four test groups: semi-purified pellet diet + sCSDS, non-purified pellet diet + sCSDS, semipurified diet + control (no sCSDS), and non-purified diet + control.
Results: Although increased body weight, and food and water intake following sCSDS exposure were consistently observed in the groups that were fed semi-and non-purified diets, social avoidance behavior was influenced by food type (i.e., sCSDS mice fed semi-purified diet showed the greatest social avoidance behavior). In addition, the rates of stress susceptibility were estimated at 73.9 and 34.8% in sCSDS mice fed semi-purified and non-purified diets, respectively (P < 0.05). For comparison, the susceptible-like phenotype rates were estimated at 12.5 and 8.3% in healthy control mice fed semipurified and non-purified diets, respectively.
Discussion: These results suggest that diet quality affects the vulnerability of mice to social defeat stress., TAYLOR & FRANCIS LTD
NUTRITIONAL NEUROSCIENCE, 2016年, ［査読有り］

Subchronic and mild social defeat stress alter mouse nest building behavior
Hikari Otabi; Tatsuhiko Goto; Tsuyoshi Okayama; Daisuke Kohari; Atsushi Toyoda, Behavioral and physiological evaluations of animal models of depression are essential to thoroughly understand the mechanisms of depression in humans. Various models have been developed and characterized, and the socially defeated mouse has been widely used for studying depression. Here, we developed and characterized a mouse model of social aversion using a subchronic and mild social defeat stress (sCSDS) paradigm. Compared to control mice, sCSDS mice showed significantly increased body weight gain, water intake, and social aversion to dominant mice on the social interaction test. We observed nest building behavior in sCSDS mice using the pressed cotton as a nest material. Although sCSDS mice eventually successfully built nests, the onset of nest building was severely delayed compared to control mice. The underlying mechanism of this significant delay in nest building by sCSDS mice is unclear. However, our results demonstrate that nest building evaluation is a simple and useful assay for understanding behavior in socially defeated mice and screening drugs such as antidepressants. (c) 2015 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
BEHAVIOURAL PROCESSES, 2016年01月, ［査読有り］

A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits
Tatsuhiko Goto; Atsushi Toyoda, Stressful life events often increase the incidence of depression in humans. To study the mechanisms of depression, the development of animal models of depression is essential. Because there are several types of depression, various animal models are needed for a deeper understanding of the disorder. Previously, a mouse model of subchronic and mild social defeat stress (sCSDS) using a modified chronic social defeat stress (CSDS) paradigm was established. In the paradigm, to reduce physical injuries from aggressors, the duration of physical contact between the aggressor and a subordinate was reduced compared to in the original CSDS paradigm. sCSDS mice showed increased body weight gain, food intake, and water intake during the stress period, and their social behaviors were suppressed after the stress period. In terms of the face validity of the stress-induced overeating and overdrinking following the increased body weight gain, the sCSDS mice may show some features related to atypical depression in humans. Thus, a mouse model of sCSDS may be useful for studying the pathogenic mechanisms underlying depression. This protocol will help establish the sCSDS mouse model, especially for studying the mechanisms underlying stress-induced weight gain and polydipsia-and hyperphagia-like symptoms., JOURNAL OF VISUALIZED EXPERIMENTS
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015年11月, ［査読有り］

Strain differences in temporal changes of nesting behaviors in C57BL/6N, DBA/2N, and their F-1 hybrid mice assessed by a three-dimensional monitoring system
Tatsuhiko Goto; Tsuyoshi Okayama; Atsushi Toyoda, Nest building is one of the innate behaviors that are widely observed throughout the animal kingdom. Previous studies have reported specific brain regions and genetic loci associated with nest building in mice. These studies mainly evaluated the nest structure, without observing the nesting process. In this study, we evaluated the effects of strain and learning on the nesting process of mice using a 3D depth camera. To determine the quality of the nest structure, a conventional scoring method, Deacon scores 1-5, was applied to the recorded depth images. The final score of the nest, latency to start nesting behavior, and latencies to reach Deacon scores 3-5, were determined using three genetically different mouse strains-C57BL/6NCrl (B6), DBA/2NCrICrlj (DBA), and B6D2F1/Crl (B6D2F1). The final score of the DBA nest was significantly lower than that of the B6D2F1 nest, and DBA mice showed significantly longer latency to start nest building than the other two strains in the first trial. By observing the time course of nest building, we confirmed that DBA mice took significantly longer to build their nests than B6 and B6D2F1 mice. Although we did not find any significant differences between DBA and B6 mice in the final assessment of the nest based on the Deacon method, overnight monitoring of the nesting behavior using a 3D depth camera could elucidate the clear differences in the amount of time spent nesting between DBA and B6 mice. In addition, the learning effect was more evident in DBA mice than it was in B6 in terms of latencies to reach Deacon score 3-5 in five repeated trials. DBA mice showed a gradual decrease in latency to build, whereas nesting behaviors of B6 mice were relatively consistent throughout the five trials. Therefore, our 3D depth image method gives higher resolution and structural information regarding the nesting process in mice. Future genetic analyses using the 3D assessment system will provide novel insights into the complex genetic basis for nesting and other behaviors in animals. (C) 2015 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
BEHAVIOURAL PROCESSES, 2015年10月, ［査読有り］

Assessing nest-building behavior of mice using a 3D depth camera
Tsuyoshi Okayama; Tatsuhiko Goto; Atsushi Toyoda, We developed a novel method to evaluate the nest-building behavior of mice using an inexpensive depth camera. The depth camera clearly captured nest-building behavior. Using three-dimensional information from the depth camera, we obtained objective features for assessing nest-building behavior, including "volume," "radius," and "mean height". The "volume" represents the change in volume of the nesting material, a pressed cotton square that a mouse shreds and untangles in order to build its nest. During the nest-building process, the total volume of cotton fragments is increased. The "radius" refers to the radius of the circle enclosing the fragments of cotton. It describes the extent of nesting material dispersion. The "radius" averaged approximately 60 mm when a nest was built. The "mean height" represents the change in the mean height of objects. If the nest walls were high, the "mean height" was also high. These features provided us with useful information for assessment of nest-building behavior, similar to conventional methods for the assessment of nest building. However, using the novel method, we found that JF1 mice built nests with higher walls than B6 mice, and B6 mice built nests faster than JF1 mice. Thus, our novel method can evaluate the differences in nest-building behavior that cannot be detected or quantified by conventional methods. In future studies, we will evaluate nest-building behaviors of genetically modified, as well as several inbred, strains of mice, with several nesting materials. (C) 2015 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
JOURNAL OF NEUROSCIENCE METHODS, 2015年08月, ［査読有り］

Influence of Chronic Social Defeat Stress on Digestive System Functioning in Rats
Atsushi Toyoda; Wataru Iio; Noriko Matsukawa; Takamitsu Tsukahara, Mental disorders are caused by chronic psychosocial stress, and can cause various symptoms related to the digestive system. We focused on the conjugation of intestinal absorptive and enzymatic mechanisms between chronic social defeat stress (CSDS) model rats and healthy controls to obtain general biochemical data about the intestine of the model in this study. The small intestine was divided into three regions: proximal (PI), middle (MI), and distal (DI): mRNA expression associated with a nutrient absorption, glucose absorption activity, and activities of the digestive enzymes such as maltase, sucrase and lactase was measured. Expression of both sodium-dependent glucose transporter 1 (Sglt1) and glucose transporter 2 gene tended to be higher in the stress group compared to the control group in PI. Glucose absorption was also higher in PI of the CSDS group. Sglt1 and peptide transporter 1 gene expressions in the CSDS group were significantly higher than those in the control group in DI. Furthermore, in PI, expression of the aquaporin 1 gene was significantly higher in the CSDS group compared to the control group. Thus, absorption of some nutrients might be higher in the small intestine of the CSDS rat., CENTER ACADEMIC PUBL JAPAN
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 2015年06月, ［査読有り］

Effect of Yacon Tuber (Smallanthus sonchifolius)-derived Fructooligosaccharides on the Intestinal Flora and Immune System of OVA-sensitized BALB/c Mice
Yuji Miyaguchi; Takuya Tomatsuri; Atsushi Toyoda; Eiichi Inoue; Yasuki Ogawa, Yacon (Smallanthus sonchifolius) tuber contains fructooligosaccharides (FOS). FOS are known as prebiotics that beneficially affect the host, i.e., improve the intestinal flora balance and enhance the immunomodulatory potential. In this study, we investigated the effect of a yacon extract rich in FOS (YOS) on the intestinal flora and immune system of BALB/c mice sensitized and challenged with ovalbumin in comparison with an extract with acid-degraded FOS (YAH). During the 4-week test period, the amount of YOS intake was lower than that of YAH; however, a significant increase in body weight was not shown among any of the test groups. Sneezing events were observed in OVA-treated mice. It was found that administration of yacon FOS improved the intestinal flora. Further, it was suggested that administration of yacon-derived FOS could epidemiologically improve the Th1/Th2 cytokine balance through its prebiotic effect on the number of beneficial bacteria in the animal, KARGER
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 2015年03月, ［査読有り］

Plasma and Liver Metabolic Profiles in Mice Subjected to Subchronic and Mild Social Defeat Stress
Tatsuhiko Goto; Yoshifumi Kubota; Atsushi Toyoda, To improve the quality of life of animals, understanding of stress-induced changes is necessary. Previously, we established a subchronic and mild social defeat stress (sCSDS) model in mice, which showed significantly higher body weight gain, food intake, and water intake compared to control mice. In this study, we elucidated metabolic profiles of plasma, liver, and urine in sCSDS mice by using metabolome and biochemical analyses. There was no significant difference between defeated and control mice in the plasma metabolites. In the liver of sCSDS mice, levels of taurocyamine (GES), phosphorylcholine, d-alanyl-d-alanine (d-ala-d-ala), and 1-methylnicotinamide (MNA) were elevated compared to controls. Taurine plays a role in osmotic regulation, and GES is a potential inhibitor of the taurine transporter. The polydipsia and increased body water content in sCSDS mice may disrupt body fluid balance following GES elevation. Furthermore, sCSDS increased heart and spleen weight significantly. Because MNA and d-ala-d-ala have anti-inflammatory and hepatoprotective effects, they may reduce inflammation in the liver of sCSDS mice. Finally, suppressed excretion of urine sodium was observed in sCSDS mice. Therefore, sCSDS induces various changes in metabolite concentrations, especially related to osmoregulation and inflammation, that may be used as biomarkers for stress-induced changes in animals., AMER CHEMICAL SOC
JOURNAL OF PROTEOME RESEARCH, 2015年02月, ［査読有り］

Effects of chronic mild food restriction on behavior and the hypothalamic malonyl-CoA signaling pathway
Wataru Iio; Yuka Tokutake; Hiroaki Koike; Noriko Matsukawa; Takamitsu Tsukahara; Shigeru Chohnan; Atsushi Toyoda, Depression induces anorexia, leading to suppressed feeding behaviors and energy intake. Previously, we revealed that chronic social defeat induced a mild suppression of feeding in rats with elevated levels of hypothalamic malonyl-CoA which regulates feeding. Therefore, we attempted to elucidate the effects of chronic mild food restriction on behavior and on hypothalamic malonyl-CoA. The chronic mild food restricted rats were fed a restricted diet approximately 80% to 90% amount of diet compared to the control for 5 weeks. Ratios of restriction were adjusted with feed consumption in the chronic social defeat stressed rats. Chronic mild food restricted rats exhibited a suppression of body weight gain similar to that of the chronic social defeat stressed rats. Also these rats showed increased time spent in the center area of an open field (OF), prolonged immobility time in forced swim, increased phosphorylation of hypothalamic adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase and a decreased concentration of hypothalamic malonyl-CoA. Weight of the adrenal glands, locomotion in an OF, mitogen-activated protein kinase cascade and calcium/calmodulin-dependent protein kinases II in the hippocampus were not affected by chronic mild food restriction. Our findings suggest that chronic mild food restriction activates AMPK following a decreased hypothalamic malonyl-CoA., WILEY-BLACKWELL
ANIMAL SCIENCE JOURNAL, 2015年02月, ［査読有り］

Differential expression of genes encoding neurotrophic factors and their receptors along the septal-temporal axis of the rat hippocampus
Atsushi Toyoda; Wataru Iio; Tatsuhiko Goto; Hiroaki Koike; Takamitsu Tsukahara, The hippocampus plays a key role in learning and emotional regulation. The hippocampus' function varies along its septotemporal axis, with the septal pole being more frequently involved in spatial learning and memory, and the temporal pole playing a greater role in emotional behaviors. In this study, we present findings aimed at checking the expression level of the genes encoding neurotrophins and their receptors, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and their receptors (TrkA, TrkB and TrkC) in the hippocampus along the septotemporal axis. Using real-time PCR, several different expression patterns were observed. Remarkably, the expression of both NT-3 and TrkA genes in the septal hippocampus was higher than in the middle and temporal hippocampus. Higher expression of NT-3 and TrkA may implicate active neurogenesis in the dentate gyrus (DG) of the septal hippocampus because more neurogenesis occurs in the septal than the temporal DG of rats. Finally, the results obtained in this study emphasize the importance of choosing the hippocampal portion along its septotemporal axis for any hippocampal molecular and biochemical experimental studies., WILEY-BLACKWELL
ANIMAL SCIENCE JOURNAL, 2014年12月, ［査読有り］

Subchronic and mild social defeat stress accelerates food intake and body weight gain with polydipsia-like features in mice
Tatsuhiko Goto; Yoshifumi Kubota; Yuki Tanaka; Wataru Iio; Naoko Moriya; Atsushi Toyoda, Development and characterization of animal models of depression are essential for fully understanding the pathogenesis of depression in humans. We made and analyzed a mouse model exhibiting social deficit and hyperphagia-like behavior using a subchronic and mild social defeat stress (sCSDS) paradigm. The body weight, food and water intake of mice were monitored during a test period, and their behaviors and serum components were analyzed at two stages: immediately after the sCSDS period and 1 month after the sCSDS. The body weight and food intake of defeated mice were significantly higher than control mice at the sCSDS period, and these differences were sustained until 1 month after the sCSDS, whereas the water intake of defeated mice was significantly higher than control mice for the period of sCSDS only. Behavioral analyses revealed that the defeated mice exhibit significant social aversion to unfamiliar mice in a social interaction test and a trend of anxiety-like behavior in an elevated-plus maze test. Possibly due to polydipsia-like symptoms, defeated mice had significantly lower levels of albumin and blood urea nitrogen than control mice immediately after the sCSDS period but not at 1 month after sCSDS. The present study revealed that our sCSDS mice keep much more water in their body than control mice. This study reports the first step toward an understanding of the mechanisms of stress-induced overhydration, over-eating and resultant weight gain. (C) 2014 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
BEHAVIOURAL BRAIN RESEARCH, 2014年08月, ［査読有り］

Effects of chronic social defeat stress on peripheral leptin and its hypothalamic actions
Wataru Iio; Haruyoshi Takagi; Yasuki Ogawa; Takamitsu Tsukahara; Shigeru Chohnan; Atsushi Toyoda, Background: Suppression of body weight and symptom of anorexia are major symptoms of depression. Recently, we reported that chronic social defeat stress (CSDS) induced suppression of body weight gain and anorexic feeding behavior in rats. These abnormalities were the result of disrupted malonyl-coenzyme A (CoA) signaling pathway in the hypothalamus. However, the condition of peripheral leptin and its hypothalamic downstream signal molecules which regulate hypothalamic malonyl-CoA level in the CSDS-exposed rats (CSDS rats) is still unknown.
Results: CSDS rats showed suppressed body weight gain and food intake. The weight of the CSDS rats' epididymal white adipose tissues was decreased when compared to the control rats. The plasma cholesterol concentration was decreased significantly in the CSDS rats compared to the control rats (P < 0.05). The plasma glucose concentration was slightly decreased in the CSDS rats compared to the control rats (P < 0.1). The expression of leptin mRNA in epididymal white adipose tissues and the plasma leptin concentration were decreased in CSDS rats. Furthermore, the phosphorylation of the hypothalamic downstream signals of leptin, including extracellular signal-regulated kinase 1/2 (ERK1/2) and signal transducer and activator of transcription 3 (STAT3), was decreased in CSDS rats.
Conclusions: Our results indicated that decreased peripheral leptin expression in CSDS rats could down-regulate the hypothalamic downstream signaling pathways of leptin while suppressed food intake. These data indicate that CSDS induces the down-regulation of hypothalamic AMPK following the elevation of hypothalamic malonyl-CoA levels and is independent of peripheral leptin and glucose., BIOMED CENTRAL LTD
BMC NEUROSCIENCE, 2014年06月, ［査読有り］

The effects of oral taurine administration on behavior and hippocampal signal transduction in rats
Wataru Iio; Noriko Matsukawa; Takamitsu Tsukahara; Atsushi Toyoda, Taurine, 2-aminoethylsulfonic acid, is one of the most abundant amino acids in the brain. It has various important physiological functions as a neuromodulator and antioxidant. Taurine is expected to be involved in depression; however, knowledge regarding its function in relation to depression is limited. In this study, we attempted to elucidate the effects of oral taurine administration on antidepressant-like behaviors in rats and depression-related signal transduction in the hippocampus. In behavioral tests, rats fed a high taurine (HT: 45.0 mmol/kg taurine) diet for 4 weeks (HT4w) showed decreased immobility in the forced swim test (FS) compared to controls. However, rats fed a low taurine (LT: 22.5 mmol/kg taurine) diet for 4 weeks or an HT diet for 2 weeks (HT2w) did not show a significant difference in FS compared to controls. In biochemical analyses, the expression of glutamic acid decarboxylase (GAD) 65 and GAD67 in the hippocampus was not affected by taurine administration. However, the phosphorylation levels of extracellular signal-regulated kinase1/2 (ERK1/2), protein kinase B (Akt), glycogen synthase kinase3 beta (GSK3 beta) and cAMP response element-binding protein (CREB) were increased in the hippocampus of HT4w and HT2w rats. Phospho-calcium/calmodulin-dependent protein kinase II (CaMKII) was increased in the hippocampus of HT4w rats only. Moreover, no significant changes in these molecules were observed in the hippocampus of rats fed an HT diet for 1 day. In conclusion, our findings suggest that taurine has an antidepressant-like effect and an ability to change depression-related signaling cascades in the hippocampus., SPRINGER WIEN
AMINO ACIDS, 2012年11月, ［査読有り］

Effect of diet composition on coenzyme A and its thioester pools in various rat tissues
Yuka Tokutake; Wataru Iio; Naoki Onizawa; Yuta Ogata; Daisuke Kohari; Atsushi Toyoda; Shigeru Chohnan, Three coenzyme A (CoA) molecular species, i.e., acetyl-CoA, malonyl-CoA, and nonesterified CoA (CoASH), in 13 types of fasted rat tissue were analyzed. A relatively larger pool size of total CoA, consisting of acetyl-CoA, malonyl-CoA, and CoASH, was observed in the medulla oblongata, liver, heart, and brown adipose tissue. Focusing on changes in the CoA pool size in response to the nutrient composition of the diet given, total CoA pools in rats continuously fed a high-fat diet for 4 weeks were significantly higher in the hypothalamus, cerebellum, and kidney, and significantly lower in the liver and skeletal muscle than those of rats fed a high-carbohydrate or high-protein diet. In particular, reductions in the liver were remarkable and were caused by decreased CoASH levels. Consequently, the total CoA pool size was reduced by approximately one-fifth of the hepatic contents of rats fed the other diets. In the hypothalamus, which monitors energy balance, all three CoA molecular species measured were at higher levels when rats were fed the high-fat diet. Thus, it was of interest that feeding rats a high-fat diet affected the behaviors of CoA pools in the hypothalamus, liver, and skeletal muscle, suggesting a significant relationship between CoA pools, especially malonyl-CoA and/or CoASH pools, and lipid metabolism in vivo. (C) 2012 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012年07月, ［査読有り］

Anorexic behavior and elevation of hypothalamic malonyl-CoA in socially defeated rats
Wataru Iio; Yuka Tokutake; Noriko Matsukawa; Takamitsu Tsukahara; Shigeru Chohnan; Atsushi Toyoda, Suppression of body weight and eating disorders, such as anorexia, are one of the major symptoms of psychiatric disorders such as depression. However, the mechanisms of weight loss and reduced appetite in depressive patients and in animal models of depression are largely unknown. In this study, we characterized the mechanism of anorexia resulting from depression using socially defeated rats as an animal model of depression. Socially defeated rats showed suppressed body weight gain, enlarged adrenal glands, decreased home cage activity, decreased food intake, and increased immobility in the forced swim test. These results are representative of some of the core symptoms of depression. Simultaneously, we observed decreased levels of phosphorylated AMP-activated protein kinase (AMPK) and acetyl-coenzyme A (CoA) carboxylase (ACC) and increased levels of malonyl-CoA in the hypothalamus of socially defeated rats. Hypothalamic malonyl-CoA controlled feeding behavior and elevation of malonyl-CoA in the hypothalamus induced inhibition of food intake. Our findings suggest that the suppression of body weight gain caused by social defeat stress is caused by anorexic feeding behavior via an increased concentration of malonyl-CoA in the hypothalamus. (C) 2012 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012年05月, ［査読有り］

Effects of chronic social defeat stress on MAP kinase cascade
Wataru Iio; Noriko Matsukawa; Takamitsu Tsukahara; Daisuke Kohari; Atsushi Toyoda, Chronic psychological and social stress can cause psychiatric disorders in humans. In this study, we analyzed the mitogen-activated protein kinase (MAPK) cascade in the hippocampus of chronically socially defeated rats. The rats that were subjected to social defeat every day for 5 weeks showed physiological and behavioral changes, including a reduced rate of weight gain, enlarged adrenal glands, and increased immobility in the forced swim test without concomitant changes in locomotor activity in the open field test. Altered body weight and enlarged adrenal glands are typical symptoms of human depression. Prolonged immobility in the forced swim test indicates behavioral despair, a well-established index of depression. Because the MAPK cascade plays a pivotal role in depression, we quantified the expression of these molecules in the hippocampus of chronically defeated rats using western blot analysis. We found that phospho-MAPK kinases 1/2 (MEK1/2) and phospho-extracellular signal-regulated kinases 1/2 (ERK1/2) were decreased, whereas MAPK phosphatase-1 (MKP-1) was increased in the hippocampus of chronically defeated rats compared to the control group. These results were consistent with findings in depressed patients and other animal models of depression. In conclusion, our findings suggest that chronic psychosocial stress in Wistar rats induced depression-like behavior and downregulated the MAPK cascade in the hippocampus. (C) 2011 Elsevier Ireland Ltd. All rights reserved., ELSEVIER IRELAND LTD
NEUROSCIENCE LETTERS, 2011年10月, ［査読有り］

The effects of concentrations of lysine in media on differentiation of 3T3-L1 preadipocytes
Takahito Kyoya; Aiko Ishida; Kazuki Nakashima; Ikuyo Nakajima; Atsushi Toyoda; Yutaka Nakamura; Masaya Katsumata, Intramuscular fat content is increased by feeding of low lysine diets in pigs. Reduction in dietary lysine intake results in low plasma lysine concentration and low cytosolic lysine concentration in skeletal muscles. From these observations, we hypothesized that low plasma lysine concentration in pigs fed on low lysine diets reduced supply of lysine from blood circulation to preadipocytes, and this limited supply of lysine might promote adipocyte differentiation in porcine muscles. In order to verify the hypothesis, we investigated the effects of low concentrations of lysine in culture medium on differentiation of 3T3-L1 preadipocytes. Low concentration of lysine suppressed lipid accumulation and messenger RNA (mRNA) expression and enzyme activity of fatty acid synthase. mRNA expressions of peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein alpha (C/EBP alpha) were lower in cells cultured in low lysine medium. On the other hand, mRNA and protein expressions of C/EBP beta and C/EBP delta were not inhibited by low concentrations of lysine in culture medium. These results indicate that low lysine concentrations in culture medium inhibit differentiation of 3T3-L1 preadipocytes through inhibiting the mRNA expressions of PPAR gamma and C/EBP alpha., WILEY
ANIMAL SCIENCE JOURNAL, 2011年, ［査読有り］

Coenzyme A and its thioester pools in fasted and fed rat tissues
Yuka Tokutake; Naoki Onizawa; Hiroki Katoh; Atsushi Toyoda; Shigeru Chohnan, Levels of three coenzyme A (CoA) molecular species, i.e., nonesterified CoA (CoASH), acetyl-CoA, and malonyl-CoA, in fasted and fed rat tissues were analyzed by the acyl-CoA cycling method which makes detection possible at the pmol level. Malonyl-CoA in brain tissues readily increased with feeding, and inversely, acetyl-CoA decreased. This phenomenon occurred in the cerebral cortex, hippocampus, cerebellum, and medulla oblongata, as well as in the hypothalamus which controls energy balance by monitoring malonyl-CoA. In the non-brain tissues, the sizes of the acetyl-CoA, malonyl-CoA, and CoASH pools depended on the tissues. The total CoA pools consisting of the above three CoA species in the liver, heart, and brown adipose tissue were larger and those of the perirenal, epididymal, and ovarian adipose tissues were much smaller, compared with those of other tissues including brain tissues. In addition, the response of each CoA pool to feeding was not uniform, suggesting that the tissue-specific metabolism individually functions in the non-brain tissues. Thus, a comprehensive analysis of thirteen types of rat tissue revealed that CoA pools have different sizes and showed a different response to fasting and feeding depending on the tissue. (C) 2010 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010年11月, ［査読有り］

Effect of Bacillus subtilis var. natto Fermented Soybean on Growth Performance, Microbial Activity in the Caeca and Cytokine Gene Expression of Domestic Meat Type Chickens
Ken-ichiro Fujiwara; Makoto Yamazaki; Hiroyuki Abe; Kazuki Nakashima; Yoko Yakabe; Makoto Otsuka; Yasunobu Ohbayashi; Yukino Kato; Kazuo Namai; Atsushi Toyoda; Yuji Miyaguchi; Yutaka Nakamura, Experiments were conducted to investigate the effect of the Bacillus subtilis var. natto fermented soybean (FS) on growth performance, microbial activity in the caeca and cytokine gene expression in spleen of domestic meat type chickens. Chicks were fed commercial diets supplemented with 0 (control), 1 or 2% FS for 80 days from 0 to 80 days of age, and a fourth group of chicks were fed control diet from 0 to 27 days of age, and then fed a 2% FS diet from 28 to 80 days of age. Supplementation with FS did not decrease feed intake compared to chicks fed the control diet, and there were no significant differences in body weight gain and feed efficiency due to dietary treatment. FS supplementation had no effect oil carcass weight, breast and thigh meat composition, or abdominal fat deposition. There were no significant differences in pH. NH3-N concentration, or number of microorganisms in the cecum content in each group; however. total acetic acid concentration increased (P < 0.05) in chicks fed the 2% FS Supplemented diet from 0 to 80 days of age. Supplementation with FS did not affect IFN-gamma, IL-4 and IL-13 gene expression in spleens.
It can be concluded from this study that supplementing FS to meat type chicken diets has no deleterious effect on the growth performance. and has beneficial effects on intestinal microbial activity, as observed by increases in VFA concentration., JAPAN POULTRY SCIENCE ASSOC
JOURNAL OF POULTRY SCIENCE, 2009年04月, ［査読有り］

Isolation and Identification of Cellulose-Binding Proteins from Sheep Rumen Contents
Atsushi Toyoda; Wataru Iio; Makoto Mitsumori; Hajime Minato, To extend our understanding of the mechanisms of plant cell wall degradation in the rumen, cellulose-binding proteins (CBPs) from the contents of a sheep rumen were directly isolated and identified using a metaproteomics approach. The rumen CBPs were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and some CBPs revealed endoglucanase activities toward carboxymethyl cellulose. Using mass spectrometry analyses, four CBPs were identified and annotated as known proteins from the predominant rumen cellulolytic bacterium Fibrobacter succinogenes: tetratricopeptide repeat domain protein, OmpA family protein, fibro-slime domain protein, and cellulose-binding endoglucanase F (EGF). Another CBP was identified as the cellulosomal glycosyl hydrolase family 6 exoglucanase, Cel6A, of Piromyces equi. F. succinogenes cells expressing EGF were found to be major members of the bacterial community on the surface or at the inner surface of hay stems by immunohistochemical analyses using anti-EGF antibody. The finding that four of the five CBPs isolated and identified from sheep rumen contents were from F. succinogenes indicates that F. succinogenes is significantly involved in cellulose degradation in the rumen., AMER SOC MICROBIOLOGY
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009年03月, ［査読有り］

Effect of fermented soybean, “Natto” on the production and qualities of chicken meat
K. Fujiwara; Y. Miyaguchi; X. H. Feng; A. Toyoda; Y. Nakamura; M. Yamazaki; K. Nakashima; H. Abe, Natto is a Japanese traditional soybean product fermented by Bacillus natto. The effect of natto supplement on the production and qualities of chicken meat was studied since the effective use of various waste foods is required in Japan. Dried natto prepared by heating at 60 degrees C was added to a basic diet at an amount of below 2%. The supplementation of dried natto did not influence the weights of the carcass, breast and thigh meat, fillet or abdominal fat. Growth of the thighbone such as the length, thickness of cortex bone. and Ca/P ratio in bone ash were not altered by, the addition of natto. However, the pH of male meat decreased following the supplementation of dried natto from days 28 to 80. The water-soluble protein content in male thigh meat increased in the group fed 2% natto from days 28 to 80. Free peptides increased in male thigh meat by feeding 2% natto from days 0 to 80. The supplementation of natto increased free glutamic acid in thigh meat regardless of sex. Moreover, the supplementation of natto specifically decreased meat cholesterol in female chickens though the effect was not shown in male chickens., ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
Asia-Aust. J. Anim. Sci., 2008年12月, ［査読有り］

Effect of fermented soybean “Natto” supplement on egg production and qualities
K. Fujiwara; Y. Miyaguchi; A. Toyoda; Y. Nakamura; M. Yamazaki; K. Nakashima; H. Abe, Natto is a Japanese traditional soybean product fermented by Bacillus natto. The effects of dried fermented soybean (natto) supplement on egg production and egg qualities of layer chickens was studied with regard to the effective use of various waste foods in Japan. Dried natto, prepared by heating at 60°C, was added to a basic diet at a level of up to 3%. Forty 166-wk-old layer chickens (Rhode Island Red) were randomly divided into 4 groups and five layer chickens were used in each group with two replicates. Layer chickens in group 1 were fed a basic diet as the control. The remaining 3 groups were fed the basic diet supplemented with dried natto at levels of 1, 2, and 3% (w/w), respectively. The result did not show improvements in egg production or feed conversion ratio of layer chickens even when 3% dried natto was added to the control diet. The egg qualities including egg weight, eggshell strength and thickness, yolk color, yolk weight, albumen height, and Haugh unit were also not improved. However, the feeding of dried natto changed the cholesterol content in the egg yolk. The supplementation of dried natto showed the tendency to decrease the yolk cholesterol after 12-wk of feeding compared to the control diet though it did not change plasma cholesterol levels in the blood. On the other hand, yolk cholesterol decreased significantly after 12-wk of feeding 3% dried natto (p<
0.05)., Asian-Australasian Association of Animal Production Societies
Asia-Aust. J. Anim. Sci., 2008年, ［査読有り］

Biochemical characterization of cellulose-binding proteins (CBPA and CBPB) from the rumen cellulolytic bacterium Eubacterium cellulosolvens 5
Miho Yoshimatsu; Atsushi Toyoda; Naoki Onizawa; Yutaka Nakamura; Hajime Minato, The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-beta-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007年10月, ［査読有り］

Purification, characterization, and gene analysis of cellulase (Cel8A) from Lysobacter sp IB-9374
Jiro Ogura; Atsushi Toyoda; Taisuke Kurosawa; Ai Leng Chong; Shigeru Chohnan; Takeharu Masaki, An enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium Lysobacter sp. IB-9374, a high lysyl endopeptidase-producing strain. The enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated Cel8A. The purified Cel8A had a molecular mass of 41 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A pH optimum of 5.0 was found for the beta-1,4-glucanase activity, and pH optima of 5.0 and 7.0 were found for the chitosanase activity. Nucleotide sequencing of the Ce18A gene yielded a deduced amino acid sequence that comprises a 33-amino acid, N-terminal signal peptide and a mature enzyme consisting of a 381-residue polypeptide with a predicted molecular mass of 41,241 Da. The amino acid sequence of the Cel8A, which contains the catalytic module of glycosyl hydrolase family 8, is homologous to beta-1,3-1,4-D-glucanase from Bacillus circulans WL-12 and endoglucanase N-257 from B. circulans KSM-N257., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2006年10月, ［査読有り］

Cloning, sequencing, and expression of a Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) with novel carbohydrate-binding modules, and properties of Cel5A
K Yoda; A Toyoda; Y Mukoyama; Y Nakamura; H Minato, A novel Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) was cloned and expressed in Escherichia coli, and its enzymatic properties were characterized. The cel5A gene consists of a 3,444-bp open reading frame and encodes a 1,148-amino-acid protein with a molecular mass of 127,047 Da. Cel5A is a modular enzyme consisting of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules (CBMs), two linker sequences, and a C-terminal sequence with an unknown function. The amino acid sequences of the two catalytic modules and the two CBMs are 94% and 73% identical to each other, respectively. Two regions that consisted of one CBM and one catalytic module were tandemly connected via a linker sequence. The CBMs did not exhibit significant sequence similarity with any other CBMs. Analyses of the hydrolytic activity of the recombinant Cel5A (rCel5A) comprising the CBMs and the catalytic modules showed that the enzyme is an endoglucanase with activities with carboxymethyl cellulose, lichenan, acid-swollen cellulose, and oat spelt xylan. To investigate the functions of the CBMs and the catalytic modules, truncated derivatives of rCel5A were constructed and characterized. There were no differences in the hydrolytic activities with various polysaccharides or in the hydrolytic products obtained from cellooligosaccharides between the two catalytic modules. Both CBMs had the same substrate affinity with intact rCel5A. Removal of the CBMs from rCel5A reduced the catalytic activities with various polysaccharides remarkably. These observations show that CBMs play an important role in the catalytic function of the enzyme., AMER SOC MICROBIOLOGY
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005年10月, ［査読有り］

Cloning, nucleotide sequence and module structure of the gene encoding the cellulose-binding protein B (CBPB) of Eubacterium cellulosolvens 5
A Toyoda; M Yoshimatsu; K Takano; H Minato, The nucleotide sequence of the gene encoding the cellulose-binding protein B (CBPB) of Eubacterium cellulosolvens 5 was determined. The gene consists of an open reading frame of 3,429 nucleotides. The deduced amino acid sequence of CBPB contained one module highly similar to a catalytic module of glycosyl hydrolase family 9 (GHF9), one module partially similar to a family 3 carbohydrate-binding module (CBM3), two linkers, one module similar to a CBM of cellulose-binding protein A (CBPA) from E. cellulosolvens 5, and one module almost identical to a cell wall-binding module (CWBM) of CBPA. The module similar to GHF9 showed CMCase activity, and the modules similar to CBM3 and CBM of CBPA bound to cellulose. Moreover, the module highly similar to CWBM of CBPA bound to the cell walls prepared from E. cellulosolvens 5. The amino acid sequence of CBPB had a significant homology (64.15% sequence identity) with that of CBPA. These results suggest that cbpA and cbpB genes descended from the same ancestral cellulase gene., MICROBIOL RES FOUNDATION
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2005年08月, ［査読有り］

PCR detection of bovine mitochondrial DNA derived from meat and bone meal in feed
A Toyoda; M Nakajo; H Kawachi; T Matsui; H Yano, Because bovine meat and bone meal (MBM) is thought to be a major source of bovine spongiform encephalopathy, we developed a PCR-based method for detection of bovine MBM in animal feed. We isolated bone particles from feed containing bovine MBM using a separation technique based on specific gravity and then washed bone particles with sodium hypochlorite solution and an EDTA-proteinase K solution. The mitochondrial DNA was extracted from bone particles and amplified using PCR with cattle-specific primers. Bovine DNA was not detected in a milk replacer containing dried skim milk and dried whey, but bovine DNA was detected in the milk replacer that was mixed with bovine MBM. Other cattle-derived materials in feeds did not interfere with the selective detection of bovine MBM. This method allowed detection of bovine mitochondrial DNA in feed with 0.1% added bovine MBM. When the treatment with sodium hypochlorite was excluded, bovine DNA derived from MBM could not be distinguished from bovine DNA derived from other bovine materials. However, the exclusion of this treatment improved the detection limit of bovine MBM in feed. This method appears suitable for the selective detection of bovine MBM in feed., INT ASSOC FOOD PROTECTION
JOURNAL OF FOOD PROTECTION, 2004年12月, ［査読有り］

A possible role of cellulose-binding protein A (CBPA) in the adhesion of Eubacterium cellulosolvens 5 to cellulose
A Toyoda; K Takano; H Minato, The cellulose-binding protein A (CBPA) of Eubacterium cellulosolvens 5 is a modular enzyme comprised of a catalytic domain, a cellulose-binding domain and a cell wall-binding domain. Cellobiose-grown cells changed their adhesion ability to cellulose depending on the growth phase. On the other hand, carboxymethyl cellulose (CMC)-grown cells bound to cellulose regardless of their growth phase. The distribution of CBPA in the culture supernatant and cell fractions changed depending on the carbon source contained in the medium and growth phase. The cellobiose-grown cells harvested from the culture of the late stationary growth phase did not bind to cellulose, but their adhesion ability was recovered by treatment with recombinant CBPA. Moreover, cellobiose-grown cells harvested from the culture of an early exponential growth phase bound to cellulose, but their adhesion ability was inhibited by treatment with anti-CBPA antiserum. CBPA rapidly decreased the viscosity of CMC, indicating that CBPA was endoglucanase. The results obtained in this study indicate that CBPA plays an important role in the adhesion of E. cellulosolvens 5 cells to cellulose., MICROBIOL RES FOUNDATION
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2003年08月, ［査読有り］

Identification of the cellulose-binding and the cell wall-binding domains of Eubacterium cellulosolvens 5 celluloseb-binding protein A (CBPA)
A Toyoda; H Minato, The cellulose-binding domain (CBD) and the cell wall-binding domain (CWBD) of Eubacterium cellulosolvens 5 cellulose-binding protein A (CBPA) have been determined. The gene (cbpA) encoding CBPA and its derivatives were expressed in Escherichia coli. We were able to obtain the eight recombinant proteins and examine for their cellulose-binding ability, cell wall-binding ability and carboxymethyl cellulase (CMCase) activity. Since Five recombinant proteins, which contain the unknown domain (UD-2) located between two linker-like regions of CBPA, bound to cellulose, this region has been identified as the CBD. The CBD did not show a significant sequence similarity with any other CBDs. Moreover, the N-terminal region of CBPA showed a significant sequence similarity with a catalytic domain of glycosyl hydrolase family 9, and the recombinant proteins containing the region showed CMCase activity. Since the UD-3, which is located in the C-termnal region of CBPA, bound to the cell walls of E cellulosolvens 5, the region has been identified as the CWBD. However, the CWBD did not show a significant sequence similarity with any other proteins previously reported. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved., ELSEVIER SCIENCE BV
FEMS MICROBIOLOGY LETTERS, 2002年08月, ［査読有り］

Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein A (CBPA) of Eubacterium cellulosolvens 5
A Toyoda; H Minato, The nucleotide sequence of the gene encoding the cellulose-binding protein A (CBPA) of Eubacterium cellulosolvens 5 was determined. The gene consists of an open reading frame of 3453 nucleotides and encodes a protein of I 15 1 amino acids with a molecular mass of 126 408 Da. The deduced amino acid Sequence of CBPA contained one domain highly similar to a catalytic domain of glycosyl hydrolases belonging to family 9, two linker-like domains and four domains of unknown function. Among the four domains of unknown function, the domains 1 and 2 region had significant homology in amino acid sequence with the cellulose-binding domains in the family 9 glycosyl hydrolases. The and 2 region had significant homology in amino acid sequence with the cell ulose-binding cloned gene was inserted into in expression vector, pBAD.TOPO, and expressed in Escherichia coli as a fused protein. The fused protein was detected by immunoblotting using antiserum against CBPA. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved., ELSEVIER SCIENCE BV
FEMS MICROBIOLOGY LETTERS, 2002年02月, ［査読有り］

Presence of several cellulose-binding proteins in culture supernatant and cell lysate of Eubacterium cellulosolvens 5
A Toyoda; K Yoda; Y Nakamura; H Minato, Attempts were made to separate and characterize cellulose-binding proteins (CBPs) from both the culture supernatant and cell lysate of Eubacterium cellulosolvens 5. Once the CBPs were bound to Avicel cellulose, they were then effectively eluted with the solution containing 3.2 or 5% sodium dodecyl sulfate (SDS), but not eluted with the solution containing various kinds of carbohydrates and reagents. Namely, CBPs in both the culture supernatant and cell lysate of the bacterium bound tightly and strongly to cellulose. The SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the eluted CBPs indicated that the CBPs contained the two major proteins having the molecular weights of approximately 160 and 84 kilodaltons (kDa) and one sub-major protein having a molecular weight of approximately 140 kDa. Zymogram analysis after the SDS-PAGE of the eluted CBPs showed that two proteins exhibited the highest levels of carboxymethyl cellulose (CMCase) activity corresponding to the molecular weights of approximately 160 and 90 kDa. A major protein having the molecular weight of approximately 160 kDa exhibited a distinct CMCase activity and was designated as CBPE1. Western immunoblot analysis indicated that the proteins prepared from 16 representative strains of rumen bacteria did not cross-react with rabbit antiserum raised against CBPE1. Thus, CBPE1 may be a unique CBP that plays an important role in the adhesion of the bacterium to cellulose., MICROBIOL RES FOUNDATION
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2001年12月, ［査読有り］

Effects of the Administration of Yucca shidigera Saponins on Pigs Intestinal Microbial Population
KATSUNUMA Yu; OTSUKA Makoto; NAKAMURA Yutaka; TOYODA Atsushi; TAKADA Ryozo; MINATO Hajime, Three pigs were fed a diet supplemented with 50ppm Yucca shidigera saponins, and the remaining three pigs were given a nonsupplemented diet. The feces of the pigs were collected at 103 days of age after birth. The concentration of ammonia-N in the feces of pigs given a diet supplemented with saponins was significantly lower than the pigs given the nonsupplemented diet. The total volatile fatty acids concentration in the feces was not significantly different between the pigs given a diet supplemented with or without saponins. However, the molar proportion of the acetic acid in the feces of pigs given a diet supplemented with saponins was lower, and inversely the concentration of the propionic, butyric and n-valeric acids was higher in comparison with the pigs given the nonsupplemented diet. The total viable counts in the feces were not different between the pigs given a diet supplemented with or without saponins. Bifidobacteria, eubacteria, and staphylococci were more abundant in the feces of pigs given a diet supplemented with saponins, as compared with the pigs given the non-supplemented diet. Veillonella was less abundant in the feces of pigs given a diet supplemented with saponins than the pigs given the non-supplemented diet., Japanese Society of Animal Science
Nihon Chikusan Gakkaiho, 2000年11月, ［査読有り］

Effects of the administration of Yucca shidigera saponins on pigs intestinal microbial population
Yu Katsunuma; Makoto Otsuka; Yutaka Nakamura; Atsushi Toyoda; Ryozo Takada; Hajime Minato, Three pigs were fed a diet supplemented with 50ppm Yucca shidigera saponins, and the remaining three pigs were given a nonsupplemented diet. The feces of the pigs were collected at 103 days of age after birth. The concentration of ammonia-N in the feces of pigs given a diet supplemented with saponins was significantly lower than the pigs given the nonsupplemented diet. The total volatile fatty acids concentration in the feces was not significantly different between the pigs given a diet supplemented with or without saponins. However, the molar proportion of the acetic acid in the feces of pigs given a diet supplemented with saponins was lower, and inversely the concentration of the propionic, butyric and n-valeric acids was higher in comparison with the pigs given the nonsupplemented diet. The total viable counts in the feces were not different between the pigs given a diet supplemented with or without saponins. Bifidobacteria, eubacteria, and staphylococci were more abundant in the feces of pigs given a diet supplemented with saponins, as compared with the pigs given the non-supplemented diet. Veillonella was less abundant in the feces of pigs given a diet supplemented with saponins than the pigs given the non-supplemented diet., Japanese Society of Animal Science
Anim. Sci. J., 2000年, ［査読有り］

Effect of Yucca shidigera extract and saponins on growth of bacteria isolated from animal intestinal tract
Yu Katsunuma; Yutaka Nakamura; Atsushi Toyoda; Hajime Minato, Influence of Yucca shidigera extract and saponins on the growth of bacteria isolated from the animal intestinal tract was investigated in in vitro. Among a total of 20 strains examined, seven strains classified into 7 species of Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus hirae, Escherichia coli, Biftdobacterium thermophilum, Bifidobacterium longum and Streptococcus bovis grew well on the media containing 10% Yucca shidigera extract. In addition, the minimum inhibitory concentration (MIC) of Y, shidigera saponins prepared from Y. shidigera extract against these 7 strains exceeded 28mg/ml. Meanwhile, the other 13 strains which include Bacteroides fragilis, Fusobacterium varium, Fusobacterium necrophorum, Clostridium perfringens, Clostridium innocuum, Clostridium sporogenes, Veillonella parvula, Propionibacterium acnes, Eubacterium aerofaciens, Selenomonas ruminantium, Peptococcus asaccharolyticus, Ruminococcus productus and Megasphaera elsdenii did not grow on the media containing 10% Y. shidigera extract. And, the MICs of the Y. shidigera saponins against these 13 strains was in the range of 14 to 0.7mg/ml. The results obtained in this investigation provide useful and basic information for analyzing the effects of Y shidigera extract and saponins on the constitution of microflora in the animal intestinal tract., Japanese Society of Animal Science
Anim. Sci. J., 2000年, ［査読有り］

A comparison of features and the microbial constitution of the fresh feces of pigs fed diets supplemented with or without dietary microbes
Nagamine, I; J Shimada; Y Nakamura; A Toyoda; H Minato, The features and the constitution of the microbial population of fresh feces were compared between pigs fed a diet supplemented with dietary microbes and pigs given nonsupplemented diets. The former were reared on farm C and the latter on farms A and a. The concentrations of ammonia-N, indole, and skatole of fresh feces were not significantly different between pigs reared on farm C and those raised on farms A and a, but the concentrations of ammonia-N and the skatole of fresh feces were significantly different between pigs reared on farms A and a. The total VFA (volatile fatty acids) concentration of fresh feces in pigs on farm C was slightly lower than in those on farms A and a. Moreover, the molar proportion of the acetic acid in feces in pigs on farm C was lower; inversely, that of propionic and butyric acids was higher in comparison with those on farms A and a. No differences were evident in the total viable counts of feces among pigs reared on the three different farms. Clostridium perfringens was abundant in feces of pigs raised on farms A and a, but it was not detected in pigs reared on farm C. Megasphaerae, bifidobacteria, and clostridia except for C. perfringens were more abundant in the feces of pigs fed a diet supplemented with dietary microbes on farm C, compared with pigs given the nonsupplemented diets on farms A and a., MICROBIOL RES FOUNDATION
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1998年12月, ［査読有り］

BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein
M Deguchi; Y Hata; M Takeuchi; N Ide; K Hirao; IK Yao; M Irie; A Toyoda; Y Takai, PSD-95/SAP90 is a synaptic membrane-associated guanylate kinase with three PDZ, one SH3, and one guanylate kinase (GK) domain. PSD-95/SAP90 binds various proteins through the PDZ domains and organizes synaptic junctions. PSD-95/SAP90 also interacts with the postsynaptic density (PSD) fraction-enriched protein, named SAPAP (also called GKAP and DAP), through the GK domain. SAPAP is Triton X-100-insoluble and recruits PSD-95/SAP90 into the Triton X-100-insoluble fraction in the transfected cells, suggesting that SAPAP may fix PSD-95/SAP90 to the PSD. Here we report a novel protein interacting with the GK domain of PSD95/SAP90, BEGAIN. BEGAIN is specifically expressed in brain and enriched in the PSD fraction. BEGAIN is Triton X-100-soluble in the transfected cells but is recruited to the Triton X-100-insoluble fraction by SAPAP when coexpressed with PSD-95/SAP90. BEGAIN may be a novel PSD component associated with the core complex of PSD-95/SAP90 and SAPAP., AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998年10月, ［査読有り］

A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins
K Hirao; Y Hata; N Ide; M Takeuchi; M Irie; Yao, I; M Deguchi; A Toyoda; TC Sudhof; Y Takai, At synaptic junctions, pre- and postsynaptic membranes are connected by cell adhesion and have distinct structures for specialized functions. The presynaptic membranes have a machinery for fast neurotransmitter release, and the postsynaptic membranes have clusters of neurotransmitter receptors, The molecular mechanism of the assembly of synaptic junctions is not yet clear. Pioneering studies identified postsynaptic density (PSD)-95/SAP90 as a prototypic synaptic scaffolding protein to maintain the structure of synaptic junctions. PSD-95/SAP90 belongs to a family of membrane-associated guanylate kinases and binds N-methyl-D-aspartate receptors, potassium channels, and neuroligins through the PDZ domains and GKAP/SAPAP/DAP through the guanylate kinase (GK) domain. We performed here a yeast two-hybrid screening for SAPAP-interacting molecules and identified a novel protein that has an inverse structure of membrane-associated guanylate kinases with an NH2-terminal GK-like domain followed by two WW and five PDZ domains. It binds SAPAP through the GH-like domain and NMDA receptors and neuroligins through the PDZ domains. We named this protein S-SCAM (synaptic scaffolding molecule) because S-SCAM may assemble receptors and cell adhesion proteins at synaptic junctions., AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998年08月, ［査読有り］

Molecular cloning and characterization of rat Lin-10
N Ide; K Hirao; Y Hata; M Takeuchi; M Irie; Yao, I; M Deguchi; A Toyoda; H Nishioka; A Mizoguchi; Y Takai, In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 encodes a small protein with one PDZ domain. The lin-10 product has no homology to known proteins. Here, we have cloned a rat homologue of the lin-10 product and characterized it, Rat lin-10 is ubiquitously expressed in various rat tissues and distributed in both the cytosol and membrane fractions. In brain, however, rat lin-10 is distributed only in the membrane fraction and enriched in the synaptic plasma membrane and postsynaptic density fractions. These results suggest that rat lin-10 is involved at least in synaptic functions in brain. (C) 1998 Academic Press., ACADEMIC PRESS INC
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998年02月, ［査読有り］

Binding of neuroligins to PSD-95
M Irie; Y Hata; M Takeuchi; K Ichtchenko; A Toyoda; K Hirao; Y Takai; TW Rosahl; TC Sudhof, PSD-95 is a component of postsynaptic densities in central synapses, It contains three PDZ domains that localize N-methyl-D-aspartate receptor subunit 2 (NMDA2 receptor) and K+ channels to synapses. In mouse forebrain, PSD-95 bound to the cytoplasmic COOH-termini of neuroligins, which are neuronal cell adhesion molecules that interact with beta-neurexins and form intercellular junctions. Neuroligins bind to the third PDZ domain of PSD-95, whereas NMDA2 receptors and K+ channels interact with the first and second PDZ domains. Thus different PDZ domains of PSD-95 are specialized for distinct functions. PSD-95 may recruit ion channels and neurotransmitter receptors to intercellular junctions formed between neurons by neuroligins and beta-neurexins., AMER ASSOC ADVANCEMENT SCIENCE
SCIENCE, 1997年09月, ［査読有り］

SAPAPs - A family of PSD-95/SAP90-associated proteins localized at postsynaptic density
M Takeuchi; Y Hata; K Hirao; A Toyoda; M Irie; Y Takai, PSD-95/SAP90 is a member of membrane-associated guanylate kinases localized at postsynaptic density (PSD) in neuronal cells. Membrane-associated guanylate kinases are a family of signaling molecules expressed at various submembrane domains which have the PDZ (DHR) domains, the SH3 domain, and the guanylate kinase domain. PSD-95/SAP90 interacts with N-methyl-D-aspartate receptors 2A/B, Shaker-type potassium channels, and brain nitric oxide synthase through the PDZ (DHR) domains and clusters these molecules at synaptic junctions. However, neither the function of the SH3 domain or the guanylate kinase domain of PSD-95/ SAP90, nor the protein interacting with these domains has been identified. We have isolated here a novel protein family consisting of at least four members which specifically interact with PSD-95/SAP90 and its related proteins through the guanylate kinase domain, and named these proteins SAPAPs (SAP90/PSD-95-Associated Proteins). SAPAPs are specifically expressed in neuronal cells and enriched in the PSD fraction. SAPAPs induce the enrichment of PSD-95/SAP90 to the plasma membrane in transfected cells. Thus, SAPAPs may have a potential activity to maintain the structure of PSD by concentrating its components to the membrane area., AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997年05月, ［査読有り］

Possible nicotinic receptor-mediated modulation of synaptic transmission in nucleus of the solitary tract
Takuma Shiraki; Atsushi Toyoda; Haruhiko Sugino; Aiko Hori; Shigeo Kobayashi, Signal transmission from afferent nerves to neurons in the nucleus of the solitary tract (NTS) may be mediated partially by nicotinic acetylcholine receptors (nAChRs). Here, we investigated nAChR-mediated signal transmission using rat NTS slices. First, we characterized nAChRs by obtaining patch- clamp recordings from NTS neuronal cell bodies. Under whole cell voltage- clamp conditions at -60 mV, application of nicotine induced an inward current, and this effect was blocked by hexamethonium. In outside-out patch recordings, nicotine was seen to induce a hexamethonium-sensitive single- channel current. Second, we investigated nAChR-mediated signal transmission. Fast synaptic transmission mediated by nAChRs was not detected. The action of diffusible acetylcholine (ACh) on nAChRs was then tested using the outside- out patches excised from NTS neurons as probes for ACh. When the patch was placed at a distance of 20-30 μm from the cell body, single-channel currents were recorded, and these were inhibited by hexamethonium. The frequency of channel opening was increased by high-extracellular potassium concentration solution suggesting the voltage-dependent release of ACh that acts on nAChRs. These results suggested that nAChR-mediated signal transmission from sensory afferents to NTS neurons is in part mediated by diffusible ACh.
American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 1997年03月, ［査読有り］

亜鉛欠乏がラットの脳内亜鉛濃度に与える影響
豊田 淳; 白木琢磨; 矢野史子; 松井 徹; 矢野秀雄
微量栄養素研究, 1993年, ［査読有り］

動画を用いたニホンザルの3次元顔計上データ取得
岡山毅; 小針大助; 豊田淳; 西川夏美; 染谷祐也; 正藤陽久; 飯田伸弥; 川瀬啓祐
動物園学紀要, 2025年03月21日

心理社会的ストレスモデルマウスを用いたうつ病発症に関わるバイオマーカーの探索
豊田 淳
バイオインダストリー, 2024年07月12日, ［招待有り］
筆頭著者

心理社会的ストレスモデルマウスにおける肝臓代謝物の網羅解析
谷島 優平; 中村 勇; 川瀬 貴博; 塚原 隆充; 高橋 阿貴; 豊田 淳
日本畜産学会大会講演要旨集, 2023年09月
責任著者

ヘスペリジンの給与がBALB/cオスマウスの社会行動及び脳内キヌレニン経路に及ぼす影響
田中 彩加里; 佐藤 瑞穂; 川瀬 貴博; 塚原 隆充; 豊田 淳
日本栄養・食糧学会大会講演要旨集, 2023年03月

Impaired Bile Acid Synthesis in a Taurine-Deficient Cat Model.
Teruo Miyazaki; Sei-Ich Sasaki; Atsushi Toyoda; Mutsumi Shirai; Tadashi Ikegami; Akira Honda
Advances in experimental medicine and biology, 2022年

うつ病モデルのストレス感受性と給与飼料の関係性
豊田 淳
LABIO21, 2022年01月01日, ［招待有り］
筆頭著者

実験動物用飼料がマウス脳のキヌレニン濃度におよぼす影響
谷島 優平; 奥野 海良人; 中村 勇; 宮崎 照雄; 本多 彰; 豊田 淳
日本畜産学会大会講演要旨集, 2021年09月

実験動物用飼料がマウス脳のキヌレニン濃度におよぼす影響
谷島 優平; 奥野 海良人; 中村 勇; 宮崎 照雄; 本多 彰; 豊田 淳
日本畜産学会大会講演要旨集, 2021年09月

後天的タウリン欠乏による結合組織の増加
宮崎照男; 佐々木誠一; 豊田淳; 白井睦; 森下由紀雄; 池上正; 本多彰
タウリンリサーチ, 2021年09月, ［査読有り］

ウシの飼育管理作業が大学生の心理・生理的状態に及ぼす影響
安江健; 平山望; 武田愛生; 小針大助; 岡山毅; 小松崎将一; 山川百合子; 佐々木誠一; 豊田淳
日本畜産学会報, 2021年05月, ［査読有り］
ラスト(シニア)オーサー

飼料の違いが社会的敗北BALB/cマウスの行動に与える影響
石其慧太; 佐藤瑞穂; 佐藤瑞穂; 谷島優平; 豊田淳; 豊田淳
日本農芸化学会大会講演要旨集(Web), 2020年

ヘスペリジンはLPS投与モデルマウスの血中白血球および脳内キヌレニンの増加を抑制する
佐藤瑞穂; 佐藤瑞穂; 奥野海良人; 鈴木一徳; 石其慧太; 谷島優平; 豊田淳; 豊田淳
日本栄養・食糧学会大会講演要旨集, 2020年

〔主要な業績〕Nutritional interventions for promoting stress resilience: Recent progress using psychosocial stress models of rodents.
Atsushi Toyoda
Animal science journal = Nihon chikusan Gakkaiho, 2020年01月, ［査読有り］, ［招待有り］
筆頭著者

タウリン欠乏による胆汁酸代謝の変化
宮﨑照雄; 佐々木誠一; 豊田淳; 白井睦; 池上正; 本多彰
タウリンリサーチ, 2019年09月, ［査読有り］

ガラクトオリゴ糖の長期給が食事誘発性肥満モデルマウスにおよぼす影響
鈴木 一徳; 佐藤 瑞穂; 中島 聡美; 堀端 大路; 塚原 隆充; 豊田 淳
日本栄養・食糧学会大会講演要旨集, 2019年04月

The effects of taurine depletion on bile acid composition and its amino acid-conjugation in the bile of cats
Teruo Miyazaki; Sei-ich Sasaki; Atsushi Toyoda; Mutsumi Shirai; Tadashi Ikegami; Yasushi Matsuzaki; Akira Honda
FASEB JOURNAL, 2019年04月

ヘスペリジンの給与は慢性社会的敗北ストレスによる脳内キヌレニンの増加を抑制し,社会的ストレスに対する抵抗性を付与する
佐藤瑞穂; 佐藤瑞穂; 奥野海良人; 鈴木一徳; 大澤夏樹; 井上栄一; 井上栄一; 宮口右二; 宮口右二; 豊田淳; 豊田淳
日本栄養・食糧学会大会講演要旨集, 2019年

Influences of Taurine Deficiency on Bile Acids of the Bile in the Cat Model.
Miyazaki T; Sasaki SI; Toyoda A; Shirai M; Ikegami T; Matsuzaki Y; Honda A
Advances in experimental medicine and biology, 2019年, ［査読有り］

ガラクトオリゴ糖の給与が慢性社会的敗北ストレスモデルマウスのストレス抵抗性におよぼす影響
鈴木 一徳; 市川 美沙紀; 佐藤 瑞穂; 中島 聡美; 小田原 達郎; 堀端 大路; 鏡 朋和; 豊田 淳
日本栄養・食糧学会大会講演要旨集, 2018年04月

幼若期のマウスを用いた社会的敗北モデルの作成と評価
大澤夏樹; 奥野海良人; 豊田淳
日本分子生物学会年会プログラム・要旨集(Web), 2018年

タウリンの胆汁酸代謝に及ぼす作用に評価におけるネコモデルの有用性
宮崎照雄; 佐々木誠一; 豊田 淳; 白井 睦; 池上 正; 松崎靖司; 本多 彰
タウリンリサーチ, 2018年, ［査読有り］

Development of wireless sensor nodes for animals husbandry and medical applications
J. Lu; L. Zhang; S. Matsumoto; H. Hiroshima; R. Maeda; T. Gotoh; M. Sato; A. Toyoda; N. Ohkohchi
Proceedings of IEEE Sensors, 2017年12月21日, ［査読有り］

Effects of dietary intake of Lactobacillus gasseri CP2305 (CP2305) on the depressive-like behaviours in subchronic mild social defeat stress (sCSDS) model of mice
A. Toyoda; T. Goto; T. Kawase; T. Tsukahara; S. Fujiwara
NEUROGASTROENTEROLOGY AND MOTILITY, 2017年08月, ［査読有り］
筆頭著者

未熟フクレミカンの果皮給与がC57BL/6Jオスマウスの体重と代謝産物に与える影響
豊田 淳; 佐藤 瑞穂; 井上 栄一
日本栄養・食糧学会大会講演要旨集, 2017年04月

〔主要な業績〕Social defeat models in animal science: What we have learned from rodent models
Toyoda, A.
Animal Science Journal, 2017年04月, ［査読有り］, ［招待有り］
筆頭著者

家畜とのふれあいを含む農作業活動が精神疾患者の心理・生理的状態に及ぼす効果
安江 健; 竹村 紀穂; 高橋 弘美; 柴沼 沙織; 下西 ひかり; 水野 高昌; 小松崎 将一; 山川 百合子; 小針 大助; 岡山 毅; 佐々木 誠一; 豊田 淳
日本畜産学会大会講演要旨集, 2017年03月

社会的敗北ストレスモデルを用いた増体および摂食行動に関する研究
飯尾 恒; 塚原隆充; 小川恭喜; 長南 茂; 豊田 淳
栄養生理研究会報, 2017年03月, ［査読有り］, ［招待有り］

社会的敗北モデルマウスのメタボローム解析
豊田 淳; 後藤達彦; 友永省三
タウリンリサーチ, 2017年, ［査読有り］

タウリン欠乏モデルネコの作製
宮崎照雄; 佐々木誠一; 豊田淳; 白井睦; 池上正; 松﨑靖司; 本多彰
タウリンリサーチ, 2017年, ［査読有り］

Murine Depression Model and its Potential Applications for Discovering Foods and Farm Products with Antidepressant-Like Effects
Tatsuhiko Goto; Shozo Tomonaga; Tsuyoshi Okayama; Atsushi Toyoda
FRONTIERS IN NEUROSCIENCE, 2016年03月, ［査読有り］, ［招待有り］

和牛の分娩前の尾挙げ時間に関わる要因
佐藤晴香; 小針大助; 岡山毅; 豊田淳; 小迫孝実
Animal Behaviour and Management, 2016年

食肉の給与が海馬のタンパク質リン酸化および遺伝子発現に及ぼす影響
豊田 淳; 下西ひかり; 後藤達彦
平成26年度食肉に関する助成研究調査成果報告書, 2015年11月

慢性的な社会敗北ストレスはC57BL/6Jマウスの消化管機能に影響をおよぼす
守谷直子; 山岸直子; 木元広実; 大森英之; 青木綾子; 後藤達彦; 豊田淳; 鈴木チセ
日本畜産学会大会講演要旨, 2015年03月28日

社会的敗北ストレス負荷がマウスの胆汁酸産生に及ぼす影響
麻生愛花; 一法師克成; 山岸直子; 守谷直子; 青木(吉田)綾子; 後藤達彦; 豊田淳; 山本(前田)万里; 鈴木チセ
日本農芸化学会大会講演要旨集(Web), 2015年03月05日

深度センサを用いたインゲンマメの水ストレス反応の三次元計測
岡山毅; 鈴木丈詞; 豊田淳
農業環境工学関連学会合同大会講演要旨集(CD-ROM), 2015年

社会心理的ストレスモデルマウスの腸内細菌叢プロファイル
山岸直子; 青木綾子; 青木綾子; 守谷直子; 後藤達彦; 豊田淳; 豊田淳; 青木玲二; 鈴木チセ; 鈴木チセ; 高山喜晴
臨床ストレス応答学会大会抄録集, 2015年

慢性社会的敗北ストレスがマウス大腸のタイト結合構成蛋白質の遺伝子発現に与える効果
山岸直子; 青木(吉田)綾子; 守谷直子; 後藤達彦; 後藤達彦; 豊田淳; 豊田淳; 豊田淳; 青木玲二; 鈴木チセ; 高山喜晴
日本生化学会大会(Web), 2015年

Effects of Chronic Taurine Administration on Gene Expression, Protein Translation and Phosphorylation in the Rat Hippocampus
Atsushi Toyoda; Hiroaki Koike; Kouichiro Nishihata; Wataru Iio; Tatsuhiko Goto
TAURINE 9, 2015年, ［査読有り］

Monitoring Nest Building in Mice and Leaf Movement of Kidney Beans using an Infrared Range Camera
Tsuyoshi Okayama; Tatsuhiko Goto; Takeshi Suzuki; Atsushi Toyoda
International Conference on Agro-Industry (IcoA): Sustainable and Competitive Agro-Industry for Human Welfare Yogyakarta-INDONESIA 2014, 2015年, ［査読有り］

食肉の給与が海馬のタンパク質リン酸化および遺伝子発現におよぼす影響
豊田 淳
平成25年度食肉に関する助成研究成果報告書, 2014年12月

慢性社会的敗北ストレスがマウスの行動および血液生化学性状におよぼす影響
後藤達彦; 久保田祥史; 吉田綾子; 青木玲二; 高山喜晴; 守谷直子; 鈴木チセ; 豊田淳
日本畜産学会大会講演要旨, 2014年

C57BL/6Jマウスの慢性社会的敗北ストレス負荷による腸内細菌叢の変動
吉田綾子; 後藤達彦; 青木玲二; 高山喜晴; 守谷直子; 豊田淳; 鈴木チセ
日本畜産学会大会講演要旨, 2014年

腸管オルガノイド及び社会的ストレス負荷マウスを用いた精神的ストレス評価系の構築
青木(吉田)綾子; 濱田晃弘; 後藤達彦; 青木玲二; 高山喜晴; 守谷直子; 豊田淳; 鈴木チセ; 園山慶
日本栄養・食糧学会大会講演要旨集, 2014年

赤外線深度センサを利用した動物,植物の動きの解析
豊田淳; 豊田淳; 豊田淳; 後藤達彦; 後藤達彦; 荒川俊也; 小針大助; 小針大助; 小針大助; 鈴木丈詞; 岡山毅; 岡山毅; 岡山毅
日本生化学会大会(Web), 2014年

Antidepressant-like effect of chronic taurine administration and its hippocampal signal transduction in rats
Atsushi Toyoda; Wataru Iio
Advances in Experimental Medicine and Biology, 2013年, ［査読有り］

飼料中リジン濃度の低減によるブタ筋肉内脂肪の畜積促進機構の解明
京谷隆侍; 石田藍子; 中島一喜; 中島郁世; 豊田淳; 中村豊; 勝俣昌也
栄養生理研究会報, 2011年06月, ［査読有り］

Effects of chronic taurine administration on behavioral and biochemical parameters in Wistar rats
Wataru Iio; Atsushi Toyoda
NEUROSCIENCE RESEARCH, 2011年

食肉由来機能性成分クレアチンによる脳機能の維持・改善に関する研究
鬼澤直樹; 豊田裕子; 豊田淳
食肉に関する助成研究調査成果報告書, 2011年

The magnitudes of promoted accumulation of intramuscular fat in responding to dietary low lysine in five distinct skeletal muscles of fattening pigs
Kyoya, T.; Ishida, A.; Nakashima, K.; Toyoda, A.; Nakamura, Y.; Katsumata, M.
EAAP Scientific Series, 2010年, ［査読有り］

成長および低リジン飼料給与に伴う豚筋肉内脂肪細胞の数と大きさの変化
京谷隆侍; 京谷隆侍; 石田藍子; 中島一喜; 豊田淳; 豊田淳; 中村豊; 中村豊; 勝俣昌也
日本畜産学会大会講演要旨, 2010年

うつ病モデルラットの作製
豊田淳
財団法人 タカノ農芸化学研究助成財団平成21年度助成研究報告書, 2010年

食肉由来機能性成分クレアチンによる脳機能の維持・改善に関する研究
鬼澤直樹; 豊田淳
食肉に関する助成研究調査成果報告書, 2010年

スィートソルガム茎における糖収量の年次間差異
神山 朗範; 成澤 才彦; 久留主 泰朗; 太田 寛行; 長南 茂; 宮口 右二; 豊田 淳; 加藤 亮; 小林 久; 小松崎 将一; 佐藤 達雄; 新田 洋司; 吉田 貢士; 本間 貴司; 梅原 涼平; 小林 亮; 松田 智明; 中村 聡; 後藤 雄佐; 井上 栄一
日本作物学会東北支部会報, 2009年

気象条件がスィートソルガム茎における糖収量に与える影響
神山 朗範; 成澤 才彦; 久留主 泰朗; 太田 寛行; 長南 茂; 宮口 右二; 豊田 淳; 加藤 亮; 小林 久; 小松崎 将一; 佐藤 達雄; 新田 洋司; 吉田 貢士; 本間 貴司; 梅原 涼平; 小林 亮; 松田 智明; 中村 聡; 後藤 雄佐; 井上 栄一
日本作物学会東北支部会報, 2009年

低リジン飼料を豚に給与したときの筋肉内脂肪の蓄積は筋肉の種類によって異なる
京谷隆侍; 京谷隆侍; 石田藍子; 中島一喜; 豊田淳; 中村豊; 勝俣昌也
日本畜産学会大会講演要旨, 2009年

食肉由来機能性成分クレアチンによる脳機能の維持・改善に関する研究
鬼澤直樹; 豊田 淳
食肉に関する助成研究調査成果報告書, 2009年

45 バイオアルコール生産を目指したスィートソルガムの栽培制御研究(栽培,日本作物学会第225回講演会)
新田洋司; 神山朗範; 松田智明; 中村聡; 後藤雄佐; 井上栄一; 成澤才彦; 久留主泰朗; 太田寛行; 長南茂; 豊田淳; 加藤亮; 小林久; 小松崎将一; 佐藤達雄
日本作物學會紀事, 2008年03月27日

納豆残さの給与が採卵鶏および筑波地鶏の飼養成績および盲腸内環境に及ぼす影響
藤原謙一郎; 加藤由紀乃; 大林康信; 生井和夫; 磯政男; 山崎信; 阿部啓之; 中島一喜; 石田藍子; 大塚誠; 豊田淳; 宮口右二; 中村豊; 金子謙一郎
日本畜産学会大会講演要旨, 2005年

ルミノロジー研究における最近の進歩（4）
湊 一; 三森真琴; 豊田 淳
畜産の研究, 2005年

ルミノロジー研究における最近の進歩（3）
湊 一; 三森真琴; 豊田 淳
畜産の研究, 2005年

ルミノロジー研究における最近の進歩（2）
湊 一; 三森真琴; 豊田 淳
畜産の研究, 2005年

ルミノロジー研究における最近の進歩（１）
湊 一; 三森真琴; 豊田 淳
畜産の研究, 2005年

ルーメン内主要セルロース分解菌、Eubacterium cellulosolvens 5、のセルロースへの付着機構
豊田淳
栄養生理研究会報, 2004年05月, ［査読有り］

繊維質分解性ルーメン細菌のセルロースへの付着機構に関する研究の現状
湊一, 豊田淳
日本微生物生態学会誌, 2004年03月

The cell wall-anchoring module of cellulose-binding protein A from rumen cellulolytic bacterium Eubacterium cellulosolvens 5
Akio Ichihara; Atsushi Toyoda; Miho Yoshimatsu; Yutaka Nakamura; Hajime Minato
Biotechnology of lignocellulose degradation and biomass utilization., 2004年, ［査読有り］

Gene cluster for the cellulose degradation in rumen cellulolytic bacterium Eubacterium cellulosolvens 5
Miho Yoshimatsu; Atsushi Toyoda; Akio Ichihara; Yutaka Nakamura; Hajime Minato
Biotechnology of lignocellulose degradation and biomass utilization., 2004年, ［査読有り］

A possible role of cellulose-binding protein A (CBPA) in adhesion of Eubacterium cellulosolvens 5 to cellulose
Atsushi Toyoda; Kazunori Takano; Akio Ichihara; Miho Yoshimatsu; Hajime Minato
Biotechnology of lignocellulose degradation and biomass utilization., 2004年, ［査読有り］

反芻胃内セルロース分解菌の有用代謝産物に関する研究
豊田 淳; 中村 豊; 湊 一
食肉に関する助成研究調査成果報告書, 2004年

反芻胃内由来繊維分解細菌の繊維結合性因子の分子的機能解析
豊田 淳; 中村 豊; 湊 一
食肉に関する助成研究調査成果報告書, 2003年

Eubacterium cellulosolvens 5のセルロース結合性蛋白質CBPAは菌体のセルロースへの付着に必要である
豊田淳; 市原朗雄; 高野和儀; 依田一豊; 湊一
日本畜産学会大会講演要旨, 2002年

Eubacterium cellulosolvens 5のセルロース分解系遺伝子の解析
吉松美帆; 豊田 淳; 市原朗雄; 中村 豊; 湊 一
ルーメン研究会報, 2002年

ルーメン内セルロース分解菌Eubacterium cellulosolvens 5のセルロース結合性タンパク質(CBPA)のC末端側の機能解析
市原朗雄; 豊田 淳; 高野和儀; 吉松美帆; 中村 豊; 湊 一
ルーメン研究会報, 2002年

反芻胃内由来繊維分解性細菌の繊維結合因子に関する研究
豊田 淳; 中村 豊; 湊 一
食肉に関する助成研究調査成果報告書, 2002年

Eubacterium cellulosolvensの繊維分解機構
豊田淳
Proceedings of Japanese Society for Rumen Metabolism and Physiology, 2001年, ［査読有り］

豚の腸内微生物叢に及ぼすユッカ抽出物サポニン給与の影響
湊 一; 中村 豊; 豊田 淳
食肉に関する助成研究調査成果報告書, 2000年

豚の腸内微生物叢に及ぼす微生物資材投与の影響
湊 一; 中村 豊; 豊田 淳
食肉に関する助成研究調査成果報告書, 1999年

PSD-95/SAP90に結合する新しい蛋白質のクローニングとその性状解析
竹内 勝一; 畑 裕; 入江 美奈; 平尾 和世; 豊田 淳; 高井 義美
日本分子生物学会年会プログラム・講演要旨集, 1996年08月01日

麹菌の資化によるカンキツ果皮中の ポリメトシキフラボノイド含有量の増加　　　　　　　　　　　　　　　
阿武佑弥; 藤井智教; 大西賢一; 柳橋諒; 長谷川守文; 豊田淳; 望月佑哉; 井上栄一
第22回日本機能性食品医用学会総会, 2024年12月07日
20241207, 20241208

Evaluation of the effects of psychosocial stress on the level of DNA damage in mouse tissues　　　　　　　　　　　　　　　
Shinya Masugata; Aiko Tsujita; Misa Iwasaki; Atsushi Toyoda; Asako J. Nakamur
第67回日本放射線影響学会, 2024年09月25日
20240928

マウスの行動実験を通して痛感した飼料の大切さ
豊田 淳
日本畜産学会第132回大会 企業協賛シンポジウム２, 2024年09月18日

D-Ala intervention from middle age prevents age-related behavioural deficits in mice.　　　　　　　　　　　　　　　
Sumika Nishi; Atsushi Toyoda
Taiwan Society for Neuroscience, 2024年08月25日
20240823, 20240825

寒冷環境への暴露は急性社会的敗北ストレスモデルマウスの巣作り行動失調を抑制するか？　　　　　　　　　　　　　　　
Chiho Kozaw; Hikari Otabi; Atsushi Toyoda
NEURO2024, 2024年07月25日

環境エンリッチメントはマウスの行動多様性を増加させる　　　　　　　　　　　　　　　
岩崎実紗; 鈴木穂高; 中村麻子; 豊田淳
NEURO2024, 2024年07月25日

半精製飼料に配合されるカゼインの種類が 亜慢性社会的敗北ストレス負荷マウスの行動に及ぼす影響　　　　　　　　　　　　　　　
川瀬貴博; 池田裕美; 西澤秀朗; 小澤智穂; 小林洋大; 豊田淳; 塚原隆充
第78回日本栄養・食糧学会大会, 2024年05月26日

実験動物を用いた栄養とレジリエンスの関係性の解明　　　　　　　　　　　　　　　
豊田 淳
第78回日本栄養・食糧学会大会, 2024年05月25日, ［招待有り］

D-アラニンの経口摂取が中年マウスの行動に及ぼす影響　　　　　　　　　　　　　　　
西純可; 豊田淳
日本農芸化学会2024年度大会, 2024年03月25日

⼼理社会的なストレスが味蕾に及ぼす影響の検討　　　　　　　　　　　　　　　
宮崎 美沙; 豊田 淳; 吉田 悠太
第46回日本分子生物学会年会, 2023年12月07日

⼼理社会的なストレスが塩味嗜好性に及ぼす影響の検討　　　　　　　　　　　　　　　
門脇 崇; 湯澤 亜利沙; 豊田 淳; 吉田 悠太
第46回日本分子生物学会年会, 2023年12月07日

A study on the relationship between salivary miR-208b-3p and stress-induced cardiac fibrosis in male mice subjected to sub-chronic and mild social defeat stress (sCSDS)　　　　　　　　　　　　　　　
Finandya Fatihasari; Yuta Yoshida; Yuhei Yajima; Kina Kawakami; Shin-ichi Nakamura; Takamitsu Tsukahara; Katsutaka Oishi; Atsushi Toyoda
第46回日本分子生物学会年会, 2023年12月06日

幼若期における社会的隔離モデルマウスの巣作り⾏動解析　　　　　　　　　　　　　　　
礒嵜 友輔; 竹ケ原 菜月; 岩崎 実紗; 川瀬 貴博; 塚原 隆充; 遠野 雅徳; 岡山 毅; 豊田 淳
第46回日本分子生物学会年会, 2023年12月06日

心理社会的ストレスモデルにおけるストレス感受性と関連する腸内細菌叢の探索　　　　　　　　　　　　　　　
谷島優平; 中村勇; 川瀬貴博; 塚原隆充; 高橋阿貴; 豊田淳
Hindgut Club Japan2023, 2023年12月02日

心理社会的ストレスモデルマウスにおける肝臓代謝物の網羅解析
谷島優平; 中村 勇; 川瀬貴博; 塚原隆充; 高橋阿貴; 豊田 淳
日本畜産学会第131回大会, 2023年09月20日, 日本畜産学会
20230918, 20230921

D-アラニンの慢性経口摂取は中年マウスの短期および長期記憶、動機付け行動に影響する　　　　　　　　　　　　　　　
西 純可; 豊田 淳
第46回日本神経科学大会, 2023年08月03日
20230801, 20230804

マウス脳のメタボローム解析用前処理法の検討　　　　　　　　　　　　　　　
廣瀬 恵太郎; 谷島 優平; 中村 勇; 豊田 淳
第46回日本神経科学大会, 2023年08月02日
20230801, 20230804

環境エンリッチメント処置がC57BL/6J系統オスマウスの各種行動に及ぼす影響　　　　　　　　　　　　　　　
岩崎 実紗; 鈴木 穂高; 中村 麻子; 豊田 淳
第46回日本神経科学大会, 2023年08月01日
20230801, 20230804

ヘスペリジンの給与がBALB/cオスマウスの社会行動及び脳内キヌレニン経路に及ぼす影響　　　　　　　　　　　　　　　
田中彩加里; 佐藤瑞穂; 川瀬貴博; 塚原 隆充; 豊田 淳
第77回日本栄養・食糧学会大会, 2023年05月14日
20230511, 20230514

半精製飼料の原料であるカゼインの種類がオスマウスの行動におよぼす影響　　　　　　　　　　　　　　　
西澤秀朗; 小澤智穂; 川瀬貴博; 塚原隆充; 豊田 淳
第77回日本栄養・食糧学会大会, 2023年05月13日
20230511, 20230514

大腸水素はうつ病モデルマウスの非レリジエント個体に対して抗うつ作用を示す　　　　　　　　　　　　　　　
山本達朗; 奥谷紗羽; 山本恵美里; 杉山夏菜; 糸澤久美; 田邊宏基; 豊田淳; 西村直道
第127回日本解剖学会総会・全国学術集会, 2023年03月

レンコン粉末の麹化による ヌシフェリン産生機構の解明　　　　　　　　　　　　　　　
柳橋 諒; 藤井智教; 大西賢一; 長谷川守文; 豊田 淳; 望月佑哉; 井上栄一
一般社団法人園芸学会令和5年度春季大会, 2023年03月20日
2023, 2023

慢性的な睡眠障害モデルマウスの唾液におけるmiRNA-seq解析　　　　　　　　　　　　　　　
吉田悠太; 谷島優平; 藤倉祐里; 庄皓桐; 山本明花; 豊田淳; 大石勝隆
第29回 日本時間生物学会学術大会, 2022年12月03日

半精製飼料に配合するカゼインの種類がオスマウスの行動におよぼす影響
西澤秀朗; 小澤智穂; 川瀬貴博; 塚原隆充; 豊田 淳
日本畜産学会第130回大会, 2022年09月16日
20220914, 20220917

慢性的な睡眠障害モデルマウスにおける唾液microRNAプロファイルの解析　　　　　　　　　　　　　　　
吉田悠太; 谷島優平; 藤倉祐里; 庄皓桐; 山本明花; 豊田淳; 大石勝隆
第9回 日本時間栄養学会学術大会, 2022年08月26日

身体的ストレスを軽減させたプロトコルによる社会的敗北ストレスモデルマウスの確立とその行動解析
西澤秀朗; 谷島優平; 豊田 淳
Neuro2022, 2022年06月30日
20220630, 20220703

社会的敗北ストレスモデルマウスの糞便、血漿からストレスバイオマーカー探索の試み
谷島優平; 中村 勇; 川瀬貴弘; 塚原隆充; 豊田 淳
Neuro2022, 2022年06月30日
20220630, 20220703

新しいタンパク質修飾・乳酸修飾とストレス　　　　　　　　　　　　　　　
萩原 英雄; 昌子 浩孝; 大給 日香里; 豊田 淳; 加藤 薫; 波平 昌一; 宮川 剛
第1回日本科学振興協会（JAAS）総会・キックオフミーティング, 2022年06月18日
20220618, 20220624

うつ病モデルマウスを用いた大腸水素の抗うつ作用に関する行動学的分析
山本達朗; 杉山夏菜; 糸澤久美; 田邊宏基; 豊田 淳 西村直道
第76回日本栄養・食糧学会, 2022年06月12日
20220610, 20220612

タウリン⽋乏によるアミロイドーシスの発症　　　　　　　　　　　　　　　
宮﨑照雄; 佐々木誠一; 豊田淳; 白井睦; 森下由紀雄; 池上正; 本多彰
第8回国際タウリン研究会日本部会学術会議, 2022年03月08日
20220307, 20220308

加齢がC57BL/6Jオスマウスの各種行動におよぼす影響
西 純可; 礒嵜友輔; 豊田 淳
第44回日本分子生物学会年会, 2021年12月03日
20211201, 20211203

実験動物用飼料がマウス脳のキヌレニン濃度におよぼす影響　　　　　　　　　　　　　　　
谷島 優平; 奥野海良人; 中村 勇; 宮崎照雄; 本多 彰; 豊田 淳
日本畜産学会第129回大会, 2021年09月15日

麹化によるレンコン粉末の機能性成分の改変
柳橋諒; 藤井智教; 大西賢一; 長谷川守文; 豊田淳; 井上栄一
園芸学会令和3年度秋季大会, 園芸学会
20210908, 20210914

未病モデルマウスに対する福来 (フクレ) ミカン果皮摂取の有効性の解析　　　　　　　　　　　　　　　
定光祐佳; 伊藤直樹; 豊田淳; 井上栄一; 小田口浩; 清原寛章
第38回和漢医薬学会, 2021年09月04日
20210904, 20210904

〔主要な業績〕うつ病モデルマウスを用いた微生物資材の評価研究
豊田 淳
第一回ヘルスケア・フーズ研究会, 2021年08月06日, 茨城県, ［招待有り］

マウス海馬のキヌレニンレベルは飼料内容の影響を受ける
谷島 優平; 奥野海良人; 中村 勇; 宮崎照雄; 本多彰; 豊田 淳
第44回日本神経科学大会, 2021年07月30日, 日本神経科学会
20210728, 20210731

深度センサによる牛分娩前体型変化の検出
岡山毅; 小針大助; 豊田淳
日本畜産学会第128回大会, 2021年03月29日, 日本畜産学会
20210327, 20210330

情動と巣作りに対する飼育ストレスの影響：BDNF一塩基多型モデルマウスの研究　　　　　　　　　　　　　　　
小島 正己; 小西 行雄; 水井 利幸; 熊ノ郷 晴子; 大給 日香里; 豊田 淳
第43回日本神経科学大会, 2020年07月30日

３次元深度カメラによる急性社会的敗北ストレスモデルマウスにおける巣作りと社会行動の解析　　　　　　　　　　　　　　　
大給 日香里; 岡山 毅; 豊田 淳
第43回日本神経科学大会, 2020年07月30日

ヘスペリジンの給与はリポ多糖投与モデルマウスの血中白血球数と脳内キヌレニンレベルの増加を抑制する　　　　　　　　　　　　　　　
佐藤 瑞穂; 佐藤 瑞穂; 奥野 海良人; 鈴木 一徳; 石其 慧太; 谷島 優平; 豊田 淳
第43回日本神経科学大会, 2020年07月29日

飼料の違いが慢性社会的敗北ストレスモデルマウスのレジリエンスと脳のキヌレニン経路におよぼす影響　　　　　　　　　　　　　　　
谷島 優平; 佐藤 瑞穂; 石其 慧太; 奥野 海良人; 豊田 淳
第43回日本神経科学大会, 2020年07月29日

慢性社会的敗北ストレスモデルマウスを用いたビフィズス菌Bifidobacterium bifidum TMC3115株給与の評価　　　　　　　　　　　　　　　
依田一豊; 佐藤瑞穂; 原田岳; 大澤夏樹; 何方; 豊田淳
日本農芸化学会2020年度大会, 2020年03月27日
20200326, 20200328

飼料の違いが社会的敗北BALB/cマウスの行動に与える影響　　　　　　　　　　　　　　　
石其 慧太; 佐藤 瑞穂; 谷島 優平; 豊田 淳
日本農芸化学会2020年度大会, 2020年03月27日
20200325, 20200328

飼料の違いが社会的敗北BALB/cマウスの行動に与える影響（優秀発表）　　　　　　　　　　　　　　　
石其慧太; 佐藤瑞穂; 谷島優平; 豊田淳
日本農芸化学会2020年度大会, 2020年03月26日, ［招待有り］
20200325, 20200328

〔主要な業績〕ヘスペリジンは心理社会的ストレスに対するレジリエンスを向上させる
豊田 淳
ヘスペリジン研究会第11回研究発表会, 2019年12月16日, ［招待有り］

脳由来神経成長因子BDNF産生不全マウスの腸内細菌と腸管機能の解析　　　　　　　　　　　　　　　
小島正己; 大給日香里; 豊田 淳; 塚原隆充; 松井このみ
第25回Hindgut Club JAPANシンポジウム, 2019年12月07日

ガラクトオリゴ糖の長期給与が食事誘発性肥満モデルマウスの増体、盲腸内細菌叢、肝臓の代謝におよぼす影響　　　　　　　　　　　　　　　
鈴木一徳; 佐藤瑞穂; 中島聡美; 堀端大路; 塚原隆充; 豊田 淳
第25回Hindgut Club JAPANシンポジウム, 2019年12月07日

慢性および亜慢性社会的敗北ストレスモデルマウスの作製と比較
寺本朱里; 鍵和田 晴美; 堀本 勝久; 豊田 淳
第42回日本分子生物学会年会, 2019年12月03日

幼若期における慢性社会的敗北ストレスは小脳キヌレニンと盲腸内細菌叢に影響する
豊田 淳; 大澤 夏樹; 奥野 海良人; 塚原 隆充
第42回日本神経科学大会, 2019年07月25日

ガラクトオリゴ糖の長期給が食事誘発性肥満モデルマウスにおよぼす影響　　　　　　　　　　　　　　　
鈴木 一徳; 佐藤 瑞穂; 中島 聡美; 堀端 大路; 塚原 隆充; 豊田 淳
第73回日本栄養・食糧学会, 2019年05月18日

ヘスペリジンの給与は慢性社会的敗北ストレスによる脳内キヌレニンの増加を抑制し、社会的ストレスに対する抵抗性を付与する　　　　　　　　　　　　　　　
佐藤 瑞穂; 奥野 海良人; 鈴木 一徳; 大澤 夏樹; 井上 栄一; 宮口 右二; 豊田 淳
第73回日本栄養・食糧学会, 2019年05月18日

茨城県産フクレミカン（Citrus tumida hort. ex Tanaka）果実におけるフラボノイド含量　　　　　　　　　　　　　　　
藤井 智教; 長谷川 守文; 豊田 淳; 井上 栄一
第73回日本栄養・食糧学会, 2019年05月18日

〔主要な業績〕心理社会的ストレスモデル動物の栄養・代謝に関する研究
豊田 淳
日本畜産学会第125回大会（日本畜産学会賞受賞者講演）, 2019年03月28日, ［招待有り］

P. gingivalis由来LPS及び酪酸の継続的な歯周組織内接種は，マウスの行動異常を惹起する　　　　　　　　　　　　　　　
川瀬貴博; 中村進一; 豊田 淳; 落合邦康; 塚原隆充
第24回 Hindgut Club Japanシンポジウム, 2018年12月08日

ヘスペリジンの給与は慢性社会的敗北ストレスによる脳内キヌレニンの増加を抑制し、社会的ストレスに対する抵抗性（レジリエンス）を付与する　　　　　　　　　　　　　　　
佐藤瑞穂; 奥野海良人; 鈴木一徳; 大澤夏樹; 井上栄一; 宮口右二; 豊田 淳
第24回 Hindgut Club Japanシンポジウム, 2018年12月08日

幼若期慢性社会的敗北モデルマウスの作成と評価ーブリーダーの影響ー　　　　　　　　　　　　　　　
大澤夏樹; 奥野海良人; 塚原隆充; 豊田 淳
第24回 Hindgut Club Japanシンポジウム, 2018年12月08日

アビシニアコロブス（Colobus guereza）の消化管各部位における腸内細菌叢および代謝産物の網羅的解析　　　　　　　　　　　　　　　
中村進一; 川瀬貴博; 豊田 淳; 飯田伸弥; 正藤晴久; 塚原隆充
第24回 Hindgut Club Japanシンポジウム, 2018年12月08日

赤外線深度センサを用いた動物の３次元行動解析
豊田 淳; 大給 日香里; 新納 浩幸; 小針 大助; 岡山 毅
第41回日本分子生物学会年会, 2018年11月30日, ［招待有り］

幼若期のマウスを用いた社会的敗北モデルの作成と評価
大澤 夏樹; 奥野 海良人; 豊田 淳
第41回日本分子生物学会年会, 2018年11月29日

A potential screening paradigm for antidepressants using the nest building evaluation after acute social defeat stress (ASDS)
H. OTABI; T. OKAYAMA; D. KOHARI; A. TOYODA
北米神経科学会, 2018年11月05日

P. gingivalis由来LPS及び酪酸の継続的な歯周組織内接種は，マウスの行動異常を惹起する
川瀬貴博; 中村進一; 豊田 淳; 落合邦康; 塚原隆充
第61回秋季日本歯周病学会学術大会, 2018年10月26日

Dietary intake of hesperidin suppresses brain kynurenine levels and social avoidance behavior in the depression model of mouse
Mizuho Sato; Arato Okuno; Kazunori Suzuki; Natsuki Ohsawa; Eiichi Inoue; Yuji Miyaguchi; Atsushi Toyoda
The 15th International Society for Tryptophan Research (ISTRY) Conference – Tryptophan Research from Bench Research to Clinical Outcome –, 2018年09月20日

アビシニアコロブスの消化管各部位における腸内細菌叢および代謝産物の網羅的解析
中村 進一; 川瀬 貴博; 豊田 淳; 飯田 伸弥; 正藤 晴久; 塚原 隆充
第24回日本野生動物医学会大会, 2018年09月01日

Effects of dietary galacto-oligosaccharide on stress-related behaviors in a subchronic mild social defeat stress model of mice
Atsushi Toyoda; Kazunori Suzuki; Misaki Ichikawa; Mizuho Sato; Satomi Nakashima; Tatsuro Odawara; Oji Horibata; Tomokazu Kagami
FNM 2018 – 3rd Meeting of the Federation of Neurogastroenterology and Motility, 2018年08月30日

乳酸菌ヘルベティカスMCC1848の摂取が慢性社会的敗北ストレスモデルマウスの行動に及ぼす効果
前畑 葉月; 川瀬 貴博; 小林 洋大; 久原 徹哉; 清水 金忠; 豊田 淳
第41回日本神経科学大会, 2018年07月27日

ヘスペリジンの慢性給与は心理社会的ストレスに対する抵抗性を向上させる
佐藤 瑞穂; 鈴木 一徳; 大澤 夏樹; 奥野 海良人; 井上 栄一; 宮口 右二; 豊田 淳
第41回日本神経科学大会, 2018年07月27日

うつ病患者および社会的敗北ストレスモデルマウスにおける脳pHの低下
萩原 英雄; 昌子 浩孝; 大給 日香里; 豊田 淳; 宮川 剛
第41回日本神経科学大会, 2018年07月27日

Decreased brain pH in patients with depression and in a social defeat mouse model
Hideo Hagihara; Hirotaka Shoji; Hikari Otabi; Atsushi Toyoda; Tsuyoshi Miyakawa
CINP2018, 2018年06月16日

うつ病モデル動物の栄養代謝研究 −レジリエンスに関わる分子の探索−
豊田 淳
第72回日本栄養・食糧学会大会 特別講演「アミノ酸栄養に対するアニマルサイエンスからのアプローチ」, 2018年05月13日, ［招待有り］

未熟フクレミカンの果皮粉末の給与が慢性社会 的敗北モデルマウスのレジリエンスにおよぼす 影響
佐藤 瑞穂; 鈴木 一徳; 大澤 夏樹; 井上 栄一; 宮口 右二; 豊田 淳
第72回日本栄養・食糧学会大会, 2018年05月12日

ガラクトオリゴ糖の給与が慢性社会的敗北スト レスモデルマウスのストレス抵抗性におよぼす 影響
鈴木 一徳; 市川 美沙紀; 佐藤 瑞穂; 中島 聡美; 小田原 達郎; 堀端 大路; 鏡 朋和; 豊田 淳
第72回日本栄養・食糧学会大会, 2018年05月12日

社会的ストレスへのレジリエンスに関わる代謝産物の探索研究
豊田淳; 後藤達彦; 友永省三
日本畜産学会第124回大会, 2018年03月29日

社会的敗北ストレスへの耐性に関わる代謝産物の探索
豊田 淳; 後藤達彦; 友永省三
ConBio2017, 2017年12月08日

幼若期社会的敗北モデルマウスの開発と評価
大澤夏樹; 豊田 淳
第23回Hindgut Club Japanシンポジウム, 2017年12月02日

未熟フクレミカン果皮成分の給与が慢性社会的敗北モデルマウスの ストレス脆弱性におよぼす影響
佐藤瑞穂; 鈴木一徳; 大澤夏樹; 井上栄一; 宮口右二; 豊田 淳
第23回Hindgut Club Japanシンポジウム, 2017年12月02日

Diet purity impacts on the cecum microbiome but not depressive-like behaviors and plasma corticosterone levels in C57Bl/6J mice
Atsushi Toyoda; Hikari Shimonishi; Mizuho Sato; Kento Usuda; Kentaro Nagaoka
Neuroscience 2017, 2017年11月13日

Nutritional and metabolic studies on chronic social defeat models
豊田 淳
第60回日本神経化学会大会, 2017年09月08日, ［招待有り］

飼育下のオオアリクイの行動は季節によって変わるのか？　　　　　　　　　　　　　　　
中山 侑; 小針大助; 豊田 淳; 木村隆司; 奥山絵里; 中根建宣; 武田 梓
KOUDOU2017, 2017年08月31日

Effects of dietary intake of Lactobacillus gasseri CP2305 (CP2305) on the depressive-like behaviours in subchronic mild social defeat stress (sCSDS) model of mice
Atsushi Toyoda; Tatsuhiko Goto; Takahiro Kawase; Takamitsu Tsukahara; Shigeru Fujiwara
NeuroGASTRO2017, 2017年08月26日

未熟フクレミカンの果皮粉末給与が慢性社会的敗北モデルマウスの社会行動におよぼす影響
佐藤 瑞穂; 井上栄一; 宮口右二; 豊田 淳
第40回日本神経科学大会, 2017年07月22日

飼料の質がマウスの行動、血漿中コルチコステロン、盲腸内細菌叢におよぼす影響
豊田 淳; 下西 ひかり; 臼田 賢人; 永岡 謙太郎
第40回日本神経科学大会, 2017年07月20日

5-HT2a受容体拮抗薬は急性社会的敗北ストレスモデルマウスにおける巣作り遅延を部分的にレスキューする
大給 日香里; 岡山 毅; 小針 大助; 豊田 淳
第40回日本神経科学大会, 2017年07月20日

恒明条件がC57BL/6Jマウスの増体に与える影響
佐藤 瑞穂; 豊田 淳
第71回日本栄養・食糧学会大会, 2017年05月20日

未熟フクレミカンの果皮給与がC57BL/6Jオスマウスの体重と代謝産物に与える影響
豊田 淳; 佐藤 瑞穂; 井上 栄一
第71回日本栄養・食糧学会大会, 2017年05月20日

うつ病モデルマウスを用いた 栄養・代謝研究　　　　　　　　　　　　　　　
豊田 淳
第71回日本栄養・食糧学会大会・ハインドガットサテライト, 2017年05月20日, ［招待有り］

家畜とのふれあいを含む農作業活動が精神疾患者の心理・生理的状態に及ぼす効果
安江 健; 竹村; 紀穂; 高橋; 弘美; 柴沼; 沙織; 下西 ひかり; 水野; 高昌; 小松崎; 将一; 山川; 百合子; 小針; 大助; 岡山; 毅; 佐々木; 誠一; 豊田 淳
日本畜産学会第122回大会, 2017年03月28日

飼育下のオオアリクイの常同行動と個体維持行動に及ぼす影響
中山侑; 小針大助; 豊田淳; 中根建宜; 武田梓; 前外間利奈
日本家畜管理学会・応用動物行動学会２０１７年度春季合同研究発表会, 2017年03月27日

社会的敗北ストレスモデルを用いた増体および摂食行動に関する研究　　　　　　　　　　　　　　　
飯尾 恒; 塚原 隆充; 小川 恭喜; 長南 茂; 豊田 淳
2017年度家畜栄養生理研究会春季集談会, 2017年03月26日, ［招待有り］

5-HT2a受容体アンタゴニストは急性社会的敗北ストレスによる巣作り障害を部分的にレスキューする
大給日香里; 豊田 淳
第90回日本薬理学会年会, 2017年03月16日

うつ病モデル動物のメタボローム研究
豊田 淳
第一回茨城大学重点研究シンポジウム つながる微生物の不思議：農と医も？, 2017年03月09日

社会的敗北モデルマウスのメタボローム解析
豊田 淳; 後藤達彦; 友永省三
国際タウリン研究会inいばらき, 2017年02月12日

タウリン欠乏モデルネコの作製
宮崎照雄; 佐々木誠一; 豊田淳; 白井睦; 池上正; 松崎靖司; 本多彰
第三回国際タウリン研究会日本部会inいばらき, 2017年02月11日

うつ病予防機能を持つ食品探索プラットフォーム
豊田 淳
第16回 産総研・産技連LS-BT合同研究発表会, 2017年01月31日

うつ病モデルマウスを用いた栄養・代謝研究
豊田 淳
第22回Hindgut Club Japanシンポジウム, 2016年12月03日, ［招待有り］

フクレミカンの未熟果皮粉末が慢性社会的敗北モデルマウスのストレス脆弱性におよぼす影響
佐藤 瑞穂; 豊田 淳
第22回Hindgut Club Japanシンポジウム, 2016年12月03日

動物の健康モニタリングを目的とした超小型低消費電力ワイヤレスセンサノードの開発
芹澤 弘一; 魯 健; 前田 龍太郎; 佐藤 瑞穂; 豊田 淳; 早瀬 仁則; 新井 恵美
第22回Hindgut Club Japanシンポジウム, 2016年12月03日

Behavioral and metabolic characterization in subchronic social defeat model of mice
A. TOYODA; T. GOTO; H. OTABI; S. TOMONAGA; D. KOHARI; T. OKAYAMA
Neuroscience 2016, 2016年11月16日, Society for neuroscience

社会的敗北モデルマウスの栄養・代謝研究
豊田 淳
2016年度行動遺伝学研究会, 2016年10月13日

Effect of farming activity included horticulture and interacting with domestic animals on person with mental disorders
Takeshi Yasue; Nozomi Sakamoto; Masakazu Komatsuzaki; Hikari Shimonishi; Yuriko Yamakawa; Daisuke Kohari; Tsuyoshi Okayama; Seiichi Sasaki; Atsushi Toyoda
The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016年08月25日

飼料の質と社会心理ストレスがマウスの代謝産物におよぼす影響
豊田 淳; 後藤 達彦; 友永 省三
第39回日本神経科学大会, 2016年07月15日

Behavioural and metabolome characterization in socially defeated mice for understanding psychiatric disorders
Atsushi Toyoda; Tatsuhiko Goto; Hikari Otabi; Shozo Tomonaga; Daisuke Kohari; Tsuyoshi Okayama
ISAE2016, 2016年07月13日

こころの健康と食の関係　　　　　　　　　　　　　　　
豊田 淳
茨城大学公開講座「オトナのための食育講座」, 2016年07月01日

飼料組成および社会心理ストレスがマウスの血漿、肝臓および盲腸代謝産物におよぼす影響
後藤 達彦; 友永 省三; 豊田 淳
日本畜産学会第121回大会, 2016年03月29日

ウシの飼育管理作業が健常者の心理・生理的状態に及ぼす効
安江 健; 平山 望; 武田 愛生; 小針 大助; 岡山 毅; 小松崎 将一; 山川 百合子; 佐々木 誠一; 豊田 淳
日本畜産学会第121回大会, 2016年03月29日

こころと栄養―うつ病モデルマウスが教えてくれること―　　　　　　　　　　　　　　　
豊田 淳
第4回 かみね・おもしろZOOサロン, 2016年02月20日

慢性社会的敗北ストレスがマウス大腸のタイト結合構成蛋白質の遺伝子発現に与える効果
山岸 直子; 青木-吉田 綾子; 守谷 直子; 後藤 達彦; 豊田 淳; 青木 玲二; 鈴木 チセ; 高山 喜晴
第38回日本分子生物学会年会（BMB2015), 2015年12月02日

社会的敗北ストレスモデルマウスにおける巣作り行動障害のメカニズム
大給 日香里; 後藤 達彦; 岡山 毅; 小針 大助; 豊田 淳
第38回日本分子生物学会年会（BMB2015), 2015年12月01日

社会的敗北モデルマウスを用いた栄養・代謝研究
豊田 淳
第18回日本補完代替医療学会学術集会, 2015年11月28日, ［招待有り］

社会心理的ストレスモデルマウスの腸内細菌叢プロファイル
山岸直子; 守谷直子; 青木綾子; 後藤達彦; 豊田淳; 青木玲二; 鈴木チセ; 高山喜晴
臨床ストレス応答学会, 2015年11月07日

Development of the objective method for evaluating nest building behaviors in mice using a 3-D depth camera
A. TOYODA; T. GOTO; T. OKAYAMA
Society for Neuroscience 2015, 2015年10月18日

Effects of diet quality on stress-vulnerability and metabolomic profiles in chronic mild social defeat stress model of mice
T. GOTO; S. TOMONAGA; Y. KUBOTA; A. TOYODA
Society for Neuroscience 2015, 2015年10月18日

Auto-detecting cattle behaviour using IR depth-sensor camera
D.Kohari; T.Okayama; H.Sato; K.Matsuo; T.Kosako; T.Goto; A.Toyoda
ISAE2015, 2015年09月15日, The Congress of the International Society for Applied Ethology (ISAE)

赤外線深度センサによるマウス巣作り行動の3D 解析
豊田淳; 後藤達彦; 大給日香里; 岡山毅
日本畜産学会第120回大会, 2015年09月12日

社会的敗北ストレスモデルマウスの新たな行動評価法の開発巣作り行動の遅延について
大給日香里; 後藤達彦; 岡山毅; 小針大助; 豊田淳
日本畜産学会第120回大会, 2015年09月11日

社会心理ストレスに暴露されたマウスの肝臓および血漿代謝産物の変化
後藤達彦; 久保田祥史; 豊田淳
日本畜産学会第120回大会, 2015年09月11日

3次元深度センサを用いたマウス巣作り行動の評価に関する研究
豊田 淳; 後藤 達彦; 岡山 毅
第38回日本神経科学会大会, 2015年07月29日

慢性的な軽度の社会的敗北ストレスを暴露したマウスにおける血漿および肝臓のメタボローム解析
後藤 達彦; 久保田 祥史; 豊田 淳
第38回日本神経科学大会, 2015年07月29日

精製および非精製飼料がマウスの社会心理ストレスに対する脆弱性におよぼす影響　　　　　　　　　　　　　　　
後藤 達彦; 久保田 祥史; 豊田 淳
第119回日本畜産学会大会, 2015年03月29日

慢性的な社会的敗北ストレスはC57BL/6Jマウスの消化管機能に影響をおよぼす　　　　　　　　　　　　　　　
守谷直子; 山岸直子; 木元広実; 大森英之; 青木綾子; 後藤達彦; 豊田淳; 鈴木チセ
第119回日本畜産学会大会, 2015年03月29日

赤外線深度センサを活用したマウス巣作り行動の解析　　　　　　　　　　　　　　　
豊田淳; 後藤達彦; 岡山毅
第119回日本畜産学会大会, 2015年03月29日

ウシの飼育管理作業がヒトの精神的・生理的状態に及ぼす影響　　　　　　　　　　　　　　　
安江 健; 武田 愛生; 小針 大助; 岡山 毅; 小松崎 将一; 豊田 淳
第119回日本畜産学会大会, 2015年03月29日

社会的敗北ストレス負荷がマウスの胆汁酸産生に及ぼす影響　　　　　　　　　　　　　　　
麻生 愛花; 一法師 克成; 山岸 直子; 守谷 直子; 青木(吉田) 綾子; 後藤 達彦; 豊田 淳; 山本(前田) 万里; 鈴木 チセ
日本農芸化学会 2015 年度大会, 2015年03月26日

茨城大学農医連携プロジェクトでの取組について　　　　　　　　　　　　　　　
豊田 淳
食と健康・新たな機能性表示制度に関する講演会, 2015年02月19日, いばらき成長産業振興協議会食品研究会, ［招待有り］

社会心理的ストレス暴露によるマウスの行動および血液性状への影響
後藤達彦; 久保田祥文; 豊田 淳
第20回Hindgut Club Japanシンポジウム, 2014年12月06日

20日間の慢性社会的敗北ストレス暴露がマウスの行動および体水分率におよぼす影響
大給 日香里; 後藤 達彦; 守谷 直子; 豊田 淳
第37回日本分子生物学会年会, 2014年11月26日

Monitoring Nest Building in Mice and Leaf Movement of Kidney Beans using an Infrared Range Camera　　　　　　　　　　　　　　　
Tsuyoshi Okayama; Tatsuhiko Goto; Takeshi Suzuki; Atsushi Toyoda
International Conference on Agro-industry, 2014年11月24日, ［招待有り］

Effects of chronic mild social defeat stress on characteristics of behaviors and serum components in mice
T. GOTO; Y. KUBOTA; Y. TANAKA; W. IIO; A. TOYODA
Neuroscience 2014, 2014年11月19日, Society for neuroscience

Three-dimensional (3-D) behavior analysis for mice using the commercial depth sensor
A. TOYODA; T. GOTO; T. OKAYAMA
Neuroscience 2014, 2014年11月15日, Society for neuroscience

ラクトフェリンの慢性的投与が社会的敗北ストレスモデルマウスの行動におよぼす影響
後藤 達彦; 久保田祥史; 豊田淳
日本ラクトフェリン学会第6回学術集会, 2014年11月08日

3D センサを用いたマウスの巣作り行動の解析 －遺伝的な影響の検討－
後藤達彦; 岡山毅; 豊田淳
日本動物遺伝育種学会第 15 回大会, 2014年11月01日

赤外線深度センサを利用した動物、植物の動きの解析
豊田 淳; 後藤 達彦; 荒川 俊也; 小針 大助; 鈴木 丈詞; 岡山 毅
第87回日本生化学会大会, 2014年10月18日, 日本生化学会, ［招待有り］

慢性社会的敗北ストレス負荷がC57BL/6Jマウスの腸管における 遺伝子発現、腸内細菌叢および腸内代謝産物に及ぼす影響　　　　　　　　　　　　　　　
青木(吉田) 綾子; 青木 玲二; 髙山 喜晴; 守谷 直子; 後藤 達彦; 豊田 淳; 鈴木 チセ
第10回日本食品免疫学会学術大会, 2014年10月16日

3次元深度センサを用いたマウス行動解析システムの開発
豊田淳; 後藤達彦; 岡山毅
第37回日本神経科学学会, 2014年09月13日

慢性的な軽度の社会的敗北ストレスがマウスの行動特性におよぼす影響
後藤 達彦; 久保田 祥史; 田中 勇気; 飯尾 恒; 豊田 淳
第37回日本神経科学学会, 2014年09月12日

ストレスは脳、行動、遺伝子、栄養に何をもたらすか？
豊田 淳
日本科学飼料協会 第431月例研究会, 2014年07月30日, ［招待有り］

動物のこころと遺伝子
豊田 淳
茨城大学農学部オープンキャンパス模擬授業, 2014年07月19日

心身の健康を維持・改善する農医連携研究の推進　　　　　　　　　　　　　　　
豊田 淳
茨城大学農学部後援会, 2014年07月12日

腸管オルガノイド及び社会的ストレス負荷マウスを用いた精神的ストレス評価系の構築
青木(吉田)綾子; 濱田晃弘; 後藤達彦; 青木玲二; 高山喜晴; 守谷直子; 豊田淳; 鈴木チセ; 園山慶
第６８回日本栄養・食糧学会シンポジウム, 2014年05月31日

Effects of chronic taurine administration on the gene and protein expression and the phosphorylation in rat hippocampus
Atsushi Toyoda; Hiroaki Koike; Kouichiro Nishihata; Wataru Iio; Tatsuhiko Goto
XIX international Taurine meeting, 2014年05月23日

赤外線深度センサを用いた動物行動解析システムの開発
豊田 淳; 後藤 達彦; 岡山 毅
第118回日本畜産学会, 2014年03月28日, 畜産草地研究所

胎生期および乳子期のラットへのタウリン強化が成長や行動、中枢神経系遺伝子発現におよぼす影響
小池 広明; 飯尾 恒; 豊田 淳
第118回日本畜産学会, 2014年03月27日, 畜産草地研究所

慢性社会的敗北ストレスがマウスの行動および血液生化学性状におよぼす影響
後藤 達彦; 久保田 祥史; 吉田 綾子; 青木 玲二; 高山 喜晴; 守谷 直子; 鈴木 チセ; 豊田 淳
第118回日本畜産学会, 2014年03月27日, 畜産草地研究所

慢性社会的敗北ストレスがマウスの増体、摂食量、および肝臓 CoAレベルにおよぼす影響
久保田 祥史; 後藤 達彦; 萩谷 友稀; 長南 茂; 豊田 淳
第118回日本畜産学会, 2014年03月27日, 畜産草地研究所

C57BL/6J マウスの慢性社会的敗北ストレス負荷による腸内細菌叢の変動
吉田 綾子; 後藤 達彦; 青木 玲二; 高山 喜晴; 守谷 直子; 豊田 淳; 鈴木 チセ
第118回日本畜産学会, 2014年03月27日, 畜産草地研究所

Introduction of Ibaraki University Cooperation between Agriculture and Medicine (IUCAM)　　　　　　　　　　　　　　　
豊田 淳
baraki University-Kasetsart University Symposium on "Integrative Molecular Biosciences", 2013年12月13日, ［招待有り］

社会心理的ストレス暴露によるマウス消化管への影響
後藤達彦; 久保田祥史; 鈴木健太; 塚原隆充; 豊田 淳
Hindgut Club Japanシンポジウム, 2013年12月07日

赤外線深度センサを用いたマウス行動解析システムの開発　　　　　　　　　　　　　　　
後藤 達彦; 岡山 毅; 豊田 淳
日本分子生物学会, 2013年12月04日

Effects of chronic taurine administration on the protein expression and phosphorylation in rat hippocampus
豊田淳; 飯尾恒; 小池広明
北米神経科学会, 2013年11月13日

Body surface temperature measurement in the mouse using an infrared thermometer installed on the ceiling of a shelter
岡山毅; 後藤達彦; 小池広明; 飯尾恒; 豊田淳
北米神経科学会, 2013年11月10日

妊娠期のタウリン給与が産仔に与える影響の解明
小池 広明; 飯尾 恒; 豊田 裕子; 豊田 淳
日本畜産学会 第116回大会, 2013年03月28日

タウリンの長期給与がラットの行動と海馬のシグナル伝達におよぼす影響
豊田 淳; 飯尾 恒
関東畜産学会, 2012年11月10日

慢性的社会性ストレスモデル動物における摂食量および体重の変化
田中勇気; 飯尾 恒; 豊田 淳
関東畜産学会, 2012年11月10日

慢性的社会性ストレスによる摂食不振は視床下部マロニル-CoA濃度の 上昇によっておこる
飯尾 恒; 小池広明; 徳竹由華; 松川典子; 塚原隆充; 長南 茂; 豊田 淳
関東畜産学会, 2012年11月10日

Anorexia and hypothalamic malonyl- CoA signaling pathway in chronic social defeated rats
飯尾 恒; 徳竹由華; 松川典子; 塚原隆充; 長南 茂; 豊田 淳
北米神経科学会, 2012年10月17日

Chronic taurine administration and its antidepressant-like action in rats
豊田 淳; 飯尾 恒; 松川典子; 塚原隆充
北米神経科学会2012, 2012年10月16日

高脂肪食がラット組織内CoAプー ルに及ぼす影響　　　　　　　　　　　　　　　
徳竹 由華; 飯尾 恒; 豊田 淳; 長南 茂
第33回肥満学会, 2012年10月11日

動物のこころと遺伝子　　　　　　　　　　　　　　　
豊田 淳
茨城大学農学部サテライト公開授業（柏）, 2012年10月06日

社会的慢性ストレスを曝露されたラットは視床下部マロニル-CoA濃度が増加し、摂食行動が抑 制される
飯尾 恒,徳竹; 由華,松川; 典子,塚原; 隆充; 長南 茂; 豊田; 淳
第35回日本神経科学大会, 2012年09月18日

実験小動物用飼育環境の計測制御装置の試作　　　　　　　　　　　　　　　
岡山 毅; 田中勇気; 飯尾 恒; 豊田 淳
日本生物環境工学会, 2012年09月

実験小動物用飼育環境計測制御装置の開発　　　　　　　　　　　　　　　
田中 勇気; 飯尾; 恒; 豊田; 淳; 岡山 毅
日本農業環境工学関連学会2012年合同大会, 2012年09月

Modification of the Acyl-CoA Cycling Method for Analysis of CoA Pools　　　　　　　　　　　　　　　
Y. TOKUTAKE; A. SAIJO; W. IIO; Y. OGATA; A. TOYODA; S. CHOHNAN
米国微生物学会, 2012年06月19日

Behavioral and biochemical studies of antidepressant-like effects of taurine
豊田 淳; 飯尾 恒
18th International Taurine Meeting, 2012年04月

社会的慢性ストレスがラット白色脂肪および脾臓の TNF-α、IL-6、レプチンの遺伝子発現に及ぼす影響　　　　　　　　　　　　　　　
高木 良治; 飯尾 恒; 瀬尾 優太; 松川 典子; 塚原 隆充; 豊田 淳; 宮口 右二; 小川 恭喜
日本畜産学会第115回大会, 2012年03月

社会的慢性ストレスモデルにおける増体抑制メカニズムの解明　　　　　　　　　　　　　　　
飯尾 恒; 徳竹; 由華; 松川 典子; 塚原 隆充; 長南 茂; 豊田 淳
日本畜産学会第115回大会, 2012年03月

タウリンの長期間投与がWistarラットの行動および海馬のシグナル伝達に及ぼす影響　　　　　　　　　　　　　　　
飯尾 恒; 松川 典子; 塚原 隆充; 豊田 淳
第17回Hindgut Club Japan, 2011年12月

タウリンの長期給与が海馬におけるグルタミン酸脱炭酸酵素GAD65およびGAD67の発現に及ぼす影響　　　　　　　　　　　　　　　
飯尾 恒; 松川 典子; 塚原 隆充; 豊田 淳
第34回日本分子生物学会年, 2011年12月

食餌の質がラット組織内CoAプールに及ぼす影響　　　　　　　　　　　　　　　
徳竹 由華; 鬼澤 直樹; 飯尾 恒; 豊田 淳; 長南 茂
第32回日本肥満学会, 2011年09月

Wistarラットにおける慢性的タウリン投与の行動学的および生化学的評価　　　　　　　　　　　　　　　
飯尾 恒; 豊田 淳
日本神経科学会, 2011年09月

未熟フクレミカン皮の給与がマウスのうつ様および不安様行動に与える影響　　　　　　　　　　　　　　　
武本 智嗣; 高野 幸恵; 真泉 蘇代子; 豊田 淳
日本栄養食糧学会, 2011年05月

ラットへの2週間のタウリン給与がうつ様行動に与える影響　　　　　　　　　　　　　　　
飯尾 恒; 豊田 淳
日本栄養食糧学会, 2011年05月

慢性的社会性ストレスと急性ストレスラットでの小腸消化吸収能の違い　　　　　　　　　　　　　　　
松川 典子; 飯尾 恒; 豊田 淳; 中山 啓三; 原 博; 塚原 隆充
日本栄養食糧学会, 2011年05月

飼料中リジン濃度の低減によるブタ筋肉内脂肪の畜積促進機構の解明　　　　　　　　　　　　　　　
京谷隆侍; 石田藍子; 中島一喜; 中島郁世; 豊田淳; 中村豊; 勝俣昌也
家畜栄養生理研究会, 2011年05月

社会的ストレス誘導型うつ病モデルラットにおける増体抑制機序の解明　　　　　　　　　　　　　　　
松川 典子; 飯尾 恒; 豊田 淳; 中山 啓三; 原 博; 塚原 隆充
日本農芸化学会, 2011年03月

4週間のタウリン給与における抗うつ効果の行動学的評価　　　　　　　　　　　　　　　
飯尾 恒; 豊田 淳
日本農芸化学会, 2011年03月

絶食および飽食ラットにおける組織内CoAプールの動態解析　　　　　　　　　　　　　　　
徳竹 由華; 鬼澤 直樹; 村田 恵; 渡辺 央好; 角田 梨奈; 豊田 淳; 長南 茂
日本農芸化学会, 2011年03月

社会的ストレスによるうつ病モデルラットの増体抑制はなぜ起こるのか？　　　　　　　　　　　　　　　
松川典子; 飯尾恒; 豊田淳; 原博; 塚原隆充
第16回Hindgut Club Japanシンポジウム, 2010年12月

飼料としてのスイートソルガム残さの品質に及ぼす蒸煮・爆砕処理の影響　　　　　　　　　　　　　　　
石井照章; 中村豊; 宮口右二; 豊田淳; 本間貴史; 長谷川守文; 新田洋司; 久留主泰朗
関東畜産学会, 2010年11月

成長および低リジン飼料給与に伴う豚筋肉内脂肪細胞の数と大きさの変化　　　　　　　　　　　　　　　
京谷隆侍; 石田藍子; 中島一喜; 豊田淳; 中村豊; 勝俣昌也
日本畜産学会第112回大会, 2010年03月

クレアチンの経口給与が脳内CoA分子種に及ぼす影響　　　　　　　　　　　　　　　
鬼澤直樹; 徳竹由華; 長南茂; 中村豊; 豊田淳
日本畜産学会第112回大会, 2010年03月

アシル‐CoAサイクリング法によるラット組織内CoAプールの解析　　　　　　　　　　　　　　　
徳竹由華; 鬼澤直樹; 豊田淳; 長南茂
日本農芸化学会, 2010年03月

機能性食品の評価により適した、社会的ストレス誘導によるうつ病モデルラットの確立　　　　　　　　　　　　　　　
松川典子; 飯尾恒; 岸野恵理子; 藤田孝輝; 中山啓三; 塚原隆充; 豊田淳
第15回Hindgut Club Japanシンポジウム, 2009年12月

酢酸カルシウム給与は鶏ヒナ社会的隔離ストレスによる血漿中コルチコステロン濃度の上昇を抑制する　　　　　　　　　　　　　　　
菅原諭; 豊田淳; 滝川裕美; 畠山雅彦
日本畜産学会第111回大会, 2009年09月

同系統ラットを用いた社会的慢性ストレスモデルの確立および生化学的評価　　　　　　　　　　　　　　　
飯尾恒; 鬼澤直樹; 和知弘朗; 豊田淳
日本畜産学会第111回大会, 2009年09月

反芻動物のルーメン内容物に含まれるセルロース結合タンパク質に対する抗体ライブラリーの作製　　　　　　　　　　　　　　　
豊田淳; 飯尾恒; 湊 一
日本畜産学会, 2009年03月

クレアチンが摂食リズムに及ぼす影響　　　　　　　　　　　　　　　
鬼澤直樹; 藤森水絵; 中村豊; 豊田淳
日本畜産学会第110回大会, 2009年03月

納豆添加飼料給与とニワトリ脾臓中の炎症性サイトカイン遺伝子の発現　　　　　　　　　　　　　　　
杉田歩; 西野景知; 五島恵; 藤田真弓; 豊田淳; 宮口右二; 小川恭喜; 藤原謙一郎; 山崎信; 大津晴彦; 矢ヶ部陽子; 阿部啓之
日本畜産学会第110回大会, 2009年03月

低リジン飼料を豚に給与したときの筋肉内脂肪の蓄積は筋肉の種類によって異なる　　　　　　　　　　　　　　　
京谷隆侍; 石田藍子; 中島一喜; 豊田淳; 中村豊; 勝俣昌也
日本畜産学会第110回大会, 2009年03月

反芻動物のルーメン内容物に含まれるセルロース結合タンパク質に対する抗体ライブラリーの作製　　　　　　　　　　　　　　　
豊田淳; 飯尾恒; 湊 一
第81回日本生化学会、第31回日本分子生物学会合同大会, 2008年12月

Sweet sorghum cultivation as a bio-fuel crop in the suburbs of Tokyo Metropolitan area.　　　　　　　　　　　　　　　
Akinori Kamiyama; Youji Nitta; Toshiaki Matsuda; Satoshi Nakamura; Yusuke Goto; Eiichi Inoue; Kazuhiko Narisawa; Yasuro Kurusu; Hiroyuki Ohta; Shigeru Chonan; Atsushi Toyoda; Tasuku Kato; Hisashi Kobayashi; Masakazu Komatsuzaki; Tatsuo Sato
5th International Crop Science Congress & Exhibition, 2008年04月

反芻動物のルーメン内容物に含まれるセルロース結合タンパク質の分離と同定　　　　　　　　　　　　　　　
豊田淳; 飯尾恒; 湊 一
日本畜産学会, 2008年03月

Sweet sorghum cultivation as a bio-fuel crop in Ibaraki prefecture.　　　　　　　　　　　　　　　
Nitta Y; Kamiyama A; Matsuda T; Nakamura S; Goto S; Inoue E; Narisawa K; Kurusu Y; Ohta H; Chonan S; Toyoda A; Kato T; Kobayashi H; Komatsuzaki M; Sato T
日本作物学会, 2008年03月

クレアチンの脳室投与がラットの摂食行動に及ぼす影響　　　　　　　　　　　　　　　
鬼澤直樹; 中村豊; 豊田淳
日本畜産学会第109回大会, 2008年03月

同系統ラットを用いた社会的慢性ストレスモデルの確立　　　　　　　　　　　　　　　
飯尾恒; 豊田淳
日本畜産学会第109回大会, 2008年03月

反芻動物のルーメン内容物に含まれるセルロース結合性タンパク質の分離と同定　　　　　　　　　　　　　　　
豊田淳; 飯尾恒; 湊 一
日本分子生物学会, 2007年12月

ミトコンドリア外膜タンパク質Voltage-dependent anion channel (VDAC)と微小管結合タンパク質Microtubule-associated protein 2 (MAP2)の結合様式　　　　　　　　　　　　　　　
西村結; 中條潤; 豊田淳
第30回日本分子生物学会、第80回日本生化学会大会, 2007年12月

乳酸発酵残さの給与が産卵鶏のヒナの飼養成績および盲腸内環境に及ぼす影響　　　　　　　　　　　　　　　
藤原 謙一; 加藤 由紀乃; 御幡 寿; 礒 政男; 宮口 右二; 豊田 淳; 小川 恭喜; 中村 豊; 山崎 信; 矢ヶ部 陽子; 中島 一喜; 阿部 啓之; 稲葉 陸太; 山田 正人; 井 上 雄三
日本畜産学会第108回大会, 2007年09月

納豆給与が筑波地鶏の肉質に及ぼす影響　　　　　　　　　　　　　　　
宮口 右二; 馮 暁寒; 西野 景知; 藤田 真弓; 豊田 淳; 小川 恭喜; 中村 豊; 藤原 謙一郎; 加藤 由紀乃; 御幡 寿; 礒 政男; 山崎 信; 中島 一喜; 阿部 啓之; 金子 謙 一郎
日本畜産学会第108回大会, 2007年09月

ルーメン内セルロース分解菌Eubacterium cellulosolvens 5のセルラーゼCel9Dの遺伝子解析　　　　　　　　　　　　　　　
矢野由梨; 吉松美帆; 豊田淳
日本畜産学会第108回大会, 2007年09月

Biochemical characterization of cellulose-binding proteins (CBPA and CBPB) from the rumen cellulolytic bacterium Eubacterium cellulosolvens 5.　　　　　　　　　　　　　　　
Miho Yoshimatsu; Atsushi Toyoda; Yutaka Nakamura; Hajime Minato
The 6th Joint Symposium of Japan-Korea-China on Rumen Metabolism and Physiology – Improvement of rumen function for efficient animal production, 2007年07月

Creatine経口投与がラットの学習行動に及ぼす影響　　　　　　　　　　　　　　　
佐藤朱里; 鬼澤直樹; 豊田淳
日本栄養食糧学会第61回大会, 2007年05月

Creatineの経口投与がラットの自発行動、摂食行動に与える影響　　　　　　　　　　　　　　　
鬼澤直樹; 佐藤朱里; 豊田淳
日本栄養食糧学会第61回大会, 2007年05月

乾燥納豆給与が産卵鶏および肉用鶏の脾臓のサイトカイン遺伝子発現へ及ぼす影響　　　　　　　　　　　　　　　
阿部啓之; 矢ヶ部陽子; 山崎信; 中島一喜; 藤原謙一郎; 加藤由紀乃; 御幡寿; 礒政男; 宮口右二; 豊田淳; 小川恭喜; 中村豊; 金子謙一郎
日本畜産学会第107大会, 2007年03月

納豆の給与が産卵鶏の飼養成績、卵質および盲腸内環境に及ぼす影響　　　　　　　　　　　　　　　
藤原謙一郎; 加藤由紀乃; 御幡寿; 礒政男; 山崎信; 中島一喜; 矢ヶ部陽子; 阿部啓之; 豊田淳; 宮口右二; 小川恭喜; 中村豊; 金子謙一郎
日本畜産学会第107大会, 2007年03月

ニワトリ肝臓中のコレステロール関連遺伝子の発現に及ぼす乾燥納豆給与の影響　　　　　　　　　　　　　　　
藤田真弓; 宮口右二; 豊田淳; 小川恭喜; 中村豊; 藤原謙一郎; 加藤由紀乃; 御幡寿; 礒政男; 山崎信; 中島一喜; 矢ヶ部陽子; 阿部啓之; 金子謙一郎
日本畜産学会第107大会, 2007年03月

ルーメン内繊維分解細菌Eubacterium cellulosolvens 5のセルロース結合性タンパク質CBPAおよびCBPBの性状比較　　　　　　　　　　　　　　　
吉松美帆; 鬼澤直樹; 豊田淳
日本畜産学会第107大会, 2007年03月

クレアチンの経口投与がラットの自発行動、摂食行動に及ぼす影響　　　　　　　　　　　　　　　
鬼澤直樹; 豊田淳
日本畜産学会第107大会, 2007年03月

Effect of high temperature on a pig growth performance and its future prediction　　　　　　　　　　　　　　　
Atsuko Izutsu; Ryozo Takada; Makoto Otsuka; Masaya Katsumata; Atsushi Toyoda; Yutaka Nakamura
International student workshop in Ami consideration to food, life and environment in Asia, Proposals for issues from the new generation in Asia, 2006年10月

The cellulose-binding proteins in rumen cellulolytic bacterium Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
豊田淳
XIIth AAAP Animal Science Congress 2006 Busan, 2006年06月, ［招待有り］

ルーメン内繊維分解細菌Eubacterium cellulosolvens 5セルロース結合性タンパク質CBPBの性状解析　　　　　　　　　　　　　　　
吉松美帆; 鬼澤直樹; 豊田淳
日本畜産学会第106大会, 2006年03月

Eubacterium cellulosolvens 5のセルロース結合性タンパク質Aのモジュール構造が酵素活性に及ぼす影響　　　　　　　　　　　　　　　
鬼澤直樹; 吉松美帆; 豊田淳
日本畜産学会第106大会, 2006年03月

カンショ皮の給与が肉用鶏の品質及ぼす影響　　　　　　　　　　　　　　　
宮口右二; 永山理恵; 馬 暁寒; 豊田淳; 中村豊; 藤原謙一郎; 加藤由紀乃; 御幡 寿; 礒 政男; 山崎 信; 中島一喜; 阿部啓之
日本畜産学会第106大会, 2006年03月

カンショ皮の給与が筑波地鶏の血漿脂質成分および抗酸化能に及ぼす影響　　　　　　　　　　　　　　　
山崎 信; 阿部啓之; 中島一喜; 矢ヶ部陽子; 藤原謙一郎; 加藤由紀乃; 御幡 寿; 礒 政男; 豊田淳; 宮口右二; 中村豊; 金子謙一郎; 鬼澤文夫; 鬼澤宏幸
日本畜産学会第106大会, 2006年03月

カンショ皮および納豆残さの給与が筑波地鶏の飼養成績および盲腸内環境に及ぼす影響　　　　　　　　　　　　　　　
藤原謙一郎; 加藤由紀乃; 御幡 寿; 礒 政男; 山崎 信; 阿部啓之; 中島一喜; 永山理恵; 豊田淳; 宮口右二; 中村豊; 金子謙一郎; 鬼澤文夫; 鬼澤宏幸
日本畜産学会第106大会, 2006年03月

Lysobacter sp. IB-9374由来endoglucanase(Cel8A)遺伝子のクローニング　　　　　　　　　　　　　　　
小倉治朗; 鐘 愛玲; 豊田 淳; 長南 茂; 正木武治
日本農芸化学会, 2006年03月

ミトコンドリア外膜タンパク質Voltage-dependent anion channel (VDAC)結合タンパク質の探索　　　　　　　　　　　　　　　
中條潤; 向山佳宏; 鬼澤直樹; 豊田淳
第28回日本分子生物学会, 2005年12月

Ruminal cellulose degradation: a molecular and biochemical approach　　　　　　　　　　　　　　　
豊田淳
Food and environmental preservation in Asian agriculture, International symposium on academic exchange cooperation Udayana University and Ibaraki University, 2005年11月

採卵鶏への納豆残さの給与が卵質に及ぼす影響　　　　　　　　　　　　　　　
豊田 淳; 宮口右二; 永山理恵; 中村 豊; 藤原謙一郎; 加藤由紀乃; 大林康信; 生井和夫; 山崎 信; 阿部啓之; 中島一喜; 金子謙一郎
日本畜産学会, 2005年09月

納豆残さの給与が肉用鶏の品質に及ぼす影響　　　　　　　　　　　　　　　
宮口右二; 豊田 淳; 永山理恵; 中村 豊; 藤原謙一郎; 加藤由紀乃; 大林康信; 生井和夫; 山崎 信; 阿部啓之; 中島一喜; 金子謙一郎
日本畜産学会第105回大会, 2005年09月

納豆残さの給与が採卵鶏および筑波地鶏の血漿脂質成分および抗酸化能に及ぼす影響　　　　　　　　　　　　　　　
山崎 信; 阿部啓之; 中島一喜; 藤原謙一郎; 大林康信; 加藤由紀乃; 生井和夫; 豊田 淳; 宮口右二; 中村 豊; 金子謙一郎
日本畜産学会第105回大会, 2005年09月

納豆残さの給与が採卵鶏および筑波地鶏の飼養成績および盲腸内環境に及ぼす影響　　　　　　　　　　　　　　　
藤原謙一郎; 加藤由紀乃; 大林康信; 生井和夫; 礒 政男; 山崎 信; 阿部啓之; 中島一喜; 石田藍子; 大塚 誠; 豊田 淳; 宮口右二; 中村 豊; 金子謙一
日本畜産学会第105回大会, 2005年09月

Utitlization of plant materials in grass carp, Ctenopharyngodon idella　　　　　　　　　　　　　　　
Masayuki Asada; Atsushi Toyoda; Makoto Ohtsuka; Ryozo Takada; Yutaka Nakamura
The 5th Joint Symposium of Japan-Korea-China on Rumen Metabolism and Physiology – Improvement of rumen function for efficient animal production, 2005年07月

Cloning of a gene encoding endoglucanase (Cel5A) with novel carbohydrate-binding modules of Eubacterium cellulosolvens 5, and properties of Cel5A　　　　　　　　　　　　　　　
Kazutoyo Yoda; Atsushi Toyoda; Yoshihiro Mukoyama; Yutaka Nakamura; Hajime Minato
The 5th Joint Symposium of Japan-Korea-China on Rumen Metabolism and Physiology – Improvement of rumen function for efficient animal production, 2005年06月

PCR detection of bovine mitochondrial DNA derived from meat and bone meal in feed　　　　　　　　　　　　　　　
豊田淳
日本分子生物学会, 2004年12月

Technological Development for Detection of Animal Materials in Feed in Japan　　　　　　　　　　　　　　　
T. Kawashima; O. Enishi; K. Tajima; M. Amari; T. Hosokawa-Kanai; K. Tuchiya; A. Toyoda; H. Kawachi; T. Matsui; H. Yano
International symposium food and feed safety in the context of prion diseases, 2004年06月

「ルーメン内主要セルロース分解菌、Eubacterium cellulosolvens 5、のセルロースへの付着機構　　　　　　　　　　　　　　　
豊田淳
家畜栄養生理研究会春季集談会, 2004年05月, ［招待有り］

PCR detection of bovine mitochondrial DNA derived from meat and bone meal in feed　　　　　　　　　　　　　　　
豊田淳
Ibaraki - Wageningen Young Investigators’ Symposium on Gut Microbiology (IWASM), 2004年04月

Cloning, sequencing and expression of a gene encoding endoglucanase (Cel5A) of Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Kazutoyo Yoda; Atsushi Toyoda; Yutaka Nakamura; Hajime Minato
Ibaraki - Wageningen Young Investigators’ Symposium on Gut Microbiology (IWASM), 2004年04月

Analysis of gene cluster for the cellulose degradation in Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Miho Yoshimatsu; Atsushi Toyoda; Akio Ichihara; Yutaka Nakamura; Hajime Minato
Ibaraki - Wageningen Young Investigators’ Symposium on Gut Microbiology (IWASM), 2004年04月

Bacterial diversity of grass carp (Ctenopharyngodon idella) intestine by sequence analysis of 16S rDNA libraries　　　　　　　　　　　　　　　
Masayuki Asada; Atsushi Toyoda; Kiyoshi Tajima; Makoto Ohtsuka; Yutaka Nakamura
Ibaraki - Wageningen Young Investigators’ Symposium on Gut Microbiology (IWASM), 2004年04月

Eubacterium cellulosolvens 5のセルロース分解酵素Cel5A遺伝子クローニングおよび発現解析　　　　　　　　　　　　　　　
依田一豊; 豊田 淳; 中村 豊
日本畜産学会第103回大会, 2004年03月

Analysis of the gene cluster for cellulose degradation in rumen cellulolytic bacterium Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Miho Yoshimatsu; Atsushi Toyoda; Akio Ichihara; Yutaka Nakamura; Hajime Minato
第26回日本分子生物学会年会, 2003年12月

The cell wall-anchoring module of cellulose-binding protein A from rumen cellulolytic bacterium Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Akio Ichihara; Atsushi Toyoda; Miho Yoshimatsu; Yutaka Nakamura; Hajime Minato
第26回日本分子生物学会年会, 2003年12月

A possible role of cellulose-binding protein A (CBPA) in adhesion of Eubacterium cellulosolvens 5 to cellulose　　　　　　　　　　　　　　　
豊田淳
MIE BIOFORUM 2003 - Biotechnology of lignocellulose degradation and biomass utilization -, 2003年10月

The cell wall-anchoring module of cellulose-binding protein A from rumen cellulolytic bacterium Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Akio Ichihara; Atsushi Toyoda; Miho Yoshimatsu; Yutaka Nakamura; Hajime Minato
MIE BIOFORUM 2003 - Biotechnology of lignocellulose degradation and biomass utilization -, 2003年10月

Gene cluster for the cellulose degradation in rumen cellulolytic bacterium Eubacterium cellulosolvens 5　　　　　　　　　　　　　　　
Miho Yoshimatsu; Atsushi Toyoda; Akio Ichihara; Yutaka Nakamura; Hajime Minato
MIE BIOFORUM 2003 - Biotechnology of lignocellulose degradation and biomass utilization -, 2003年10月

高い組織選択性を有するPCR法による飼料中ウシ肉骨粉の検出　　　　　　　　　　　　　　　
豊田 淳; 中條 潤; 河内 浩行; 松井 徹; 矢野秀雄
日本畜産学会, 2003年09月

Eubacterium cellulosolvens 5のセルロース分解系遺伝子の解析　　　　　　　　　　　　　　　
吉松 美帆; 豊田 淳; 市原 朗雄; 中村 豊; 湊 一
日本畜産学会第101回大会, 2003年03月

反芻胃内繊維分解菌Eubacterium cellulosolvens 5のセルロース結合性タンパク質(CBPA)の菌体細胞壁への結合様式の解析　　　　　　　　　　　　　　　
市原 朗雄; 豊田 淳; 吉松 美帆; 中村 豊; 湊 一
日本畜産学会第101回大会, 2003年03月

反芻胃内セルロース分解菌Eubacterium cellulosolvens 5の新規セルロース結合性タンパク質の同定と機能解析　　　　　　　　　　　　　　　
豊田 淳; 吉松美帆; 市原朗雄; 湊 一
日本分子生物学会, 2002年12月

反芻胃内セルロース分解菌Eubacterium cellulosolvens 5のセルロース結合タンパク質(CBPA)の新規モジュール　　　　　　　　　　　　　　　
市原朗雄; 豊田 淳; 吉松美帆; 中村 豊; 湊 一
第25回日本分子生物学会年会, 2002年12月

Eubacterium cellulosolvens 5のセルロース分解系遺伝子の解析　　　　　　　　　　　　　　　
吉松美帆; 豊田 淳; 市原朗雄; 中村 豊; 湊 一
ルーメン研究会, 2002年10月

ルーメン内セルロース分解菌Eubacterium cellulosolvens 5のセルロース結合性タンパク質(CBPA)のC末端側の機能解析　　　　　　　　　　　　　　　
市原朗雄; 豊田 淳; 高野和儀; 吉松美帆; 中村 豊; 湊 一
ルーメン研究会, 2002年10月

Eubacterium cellulosolvens 5のセルロース結合性蛋白質CBPAは菌体のセルロース付着に必要である　　　　　　　　　　　　　　　
豊田淳; 湊一
日本畜産学会, 2002年03月

ルーメン細菌Wolinella succinogenesにおける亜硝酸還元酵素の特性について　　　　　　　　　　　　　　　
佐藤泰章; 豊田 淳; 湊 一
日本畜産学会第100回大会, 2002年03月

セルロース分解菌Eubacterium cellulosolvens由来セルロース結合性蛋白質(CBPE1)の新規セルロース結合モジュール　　　　　　　　　　　　　　　
豊田淳; 湊一
日本畜産学会第99回, 2001年09月

ルーメン細菌Eubacterium cellulosolvens由来のセルロース結合性蛋白質をコードする遺伝子のクローニング　　　　　　　　　　　　　　　
豊田淳; 湊一
日本畜産学会第98回, 2001年03月

Eubacterium cellulosolvensの繊維分解機構　　　　　　　　　　　　　　　
豊田淳
ルーメン研究会, 2001年03月, ［招待有り］

ルーメン細菌（Selenomonas ruminantium subsp. Lactilytica）の硝酸還元酵素の精製　　　　　　　　　　　　　　　
藤田和政; 豊田 淳; 湊 一
日本畜産学会第98回大会, 2001年03月

ルーメン細菌Eubacterium cellulosolvens 由来のセルロース結合性タンパク質の検出　　　　　　　　　　　　　　　
豊田 淳; 湊 一
日本畜産学会第97回, 2000年03月

豚腸管内微生物叢に及ぼすユッカ･サポニン投与の影響　　　　　　　　　　　　　　　
勝沼 優; 豊田 淳; 湊 一
日本畜産学会第97回大会, 2000年03月

ルーメン細菌（Selenomonas ruminantium subsp. Lactilytica）の硝酸還元酵素の精製　　　　　　　　　　　　　　　
藤田和政; 豊田 淳; 湊 一
日本畜産学会第97回大会, 2000年03月

ユッカ抽出物およびサポニンの家畜腸管内由来細菌の発育に及ぼす影響　　　　　　　　　　　　　　　
勝沼 優; 豊田 淳; 湊 一
日本畜産学会第96回大会, 1999年03月

in vitro ルーメン内におけるメタン生成と繊維質消化性に及ぼす硝酸塩の影響　　　　　　　　　　　　　　　
井手順子; 豊田 淳; 湊 一
日本畜産学会第94回大会, 1998年03月

通常飼育豚舎および低臭気豚舎内における豚新鮮糞便中微生物構成の比較　　　　　　　　　　　　　　　
長峰 泉; 豊田 淳; 湊 一
日本畜産学会第94回大会, 1998年03月

温細胞は加温でカチオン透過性を上昇させ脱分極する　　　　　　　　　　　　　　　
右京久美子; 豊田 淳; 小林茂雄; その他
第17回日本神経科学大会, 1997年11月

神経細胞接着因子NeuroliginとPSD-95/SAP90の結合　　　　　　　　　　　　　　　
入江美奈; 豊田淳; 高井義美; その他
日本生化学会, 1997年

PSD-95/SAP90に結合する新しい蛋白質のクローニングとその性状解析　　　　　　　　　　　　　　　
竹内勝一; 豊田 淳; 高井義美; その他
第69回日本生化学会大会・第19回日本分子生物学会年会合同年会, 1996年

PSD-95/SAP90関連遺伝子のクローニングとその性状解析　　　　　　　　　　　　　　　
入江 美奈; 豊田 淳; 高井義美; その他
第69回日本生化学会大会・第19回日本分子生物学会年会合同年会, 1996年

孤束核ニューロンに対するアンギオテンシンII(AII)の作用機構　　　　　　　　　　　　　　　
豊田 淳; 小林茂雄
日本神経科学会, 1994年11月

視床下部温受容ニューロンの温度感受性カチオンコンダクタンス　　　　　　　　　　　　　　　
堀あいこ; 豊田 淳; 小林茂雄; その他
第18回日本神経科学大会, 1994年11月

亜鉛欠乏がラットの脳内亜鉛濃度に与える影響　　　　　　　　　　　　　　　
豊田 淳; 白木琢磨; 矢野史子; 松井徹; 矢野秀雄
微量栄養素研究会, 1993年07月

〔主要な業績〕動物栄養飼料学特論　　　　　　　　　　　　　　　
2020年10月 - 現在
東京農工大学大学院連合農学研究科

〔主要な業績〕食生命科学演習　　　　　　　　　　　　　　　
2019年10月 - 現在
茨城大学

〔主要な業績〕動物科学総論　　　　　　　　　　　　　　　
2019年04月 - 現在
茨城大学

〔主要な業績〕動物飼料学　　　　　　　　　　　　　　　
2019年04月 - 現在
茨城大学

〔主要な業績〕AIMS講義　　　　　　　　　　　　　　　
2016年12月 - 現在
茨城大学

生化学　　　　　　　　　　　　　　　
茨城大学農学部

応用生理学　　　　　　　　　　　　　　　
茨城大学大学院農学研究科

〔主要な業績〕博士論文　　　　　　　　　　　　　　　
東京農工大学大学院連合農学研究科

農業生産科学特論I　　　　　　　　　　　　　　　
新潟大学農学部

修士課題実習　　　　　　　　　　　　　　　
茨城大学大学院農学研究科

プレゼンテーション演習　　　　　　　　　　　　　　　
茨城大学大学院農学研究科

修士課題研究　　　　　　　　　　　　　　　
茨城大学大学院農学研究科

プレゼンテーション演習　　　　　　　　　　　　　　　
茨城大学農学部

卒業課題研究　　　　　　　　　　　　　　　
茨城大学農学部

外書講読　　　　　　　　　　　　　　　
茨城大学農学部

動物生産科学実験（分担）　　　　　　　　　　　　　　　
茨城大学農学部

家畜と人間（分担）　　　　　　　　　　　　　　　
茨城大学農学部

動物生産科学概論（分担）　　　　　　　　　　　　　　　
茨城大学農学部

動物生産科学入門（分担）　　　　　　　　　　　　　　　
茨城大学農学部

飼料資源科学特論　　　　　　　　　　　　　　　
茨城大学大学院農学研究科

基礎栄養実習　　　　　　　　　　　　　　　
つくば国際大学

畜産学　　　　　　　　　　　　　　　
茨城大学農学部

動物海洋生物資源学特論第四　　　　　　　　　　　　　　　
九州大学大学院生物資源環境科学府

食品機能化学　　　　　　　　　　　　　　　
東京大学大学院農学生命科学研究科

ゼミナール（基礎）　　　　　　　　　　　　　　　
茨城大学農学部

農学基礎（生命）　　　　　　　　　　　　　　　
茨城大学農学部

飼料資源学　　　　　　　　　　　　　　　
茨城大学農学部

動物科学実験II　　　　　　　　　　　　　　　
茨城大学農学部

〔主要な業績〕動物生化学特論　　　　　　　　　　　　　　　
茨城大学農学部

大学院特別講義　　　　　　　　　　　　　　　
麻布大学大学院

〔主要な業績〕生物学I　　　　　　　　　　　　　　　
茨城大学

〔主要な業績〕Advanced animal nutrition　　　　　　　　　　　　　　　
茨城大学

ゼミナール（基礎）　　　　　　　　　　　　　　　
茨城大学

ゼミナール（発展）　　　　　　　　　　　　　　　
茨城大学

大学入門ゼミ　　　　　　　　　　　　　　　
茨城大学

動物生化学　　　　　　　　　　　　　　　
茨城大学

〔主要な業績〕細胞生物学　　　　　　　　　　　　　　　
茨城大学

〔主要な業績〕化学物質と生命　　　　　　　　　　　　　　　
茨城大学

〔主要な業績〕動物科学実験I　　　　　　　　　　　　　　　
茨城大学

2020年01月 - 現在, ヘスペリジン研究会

国際タウリン研究会

ルーメン研究会

北米神経科学会

日本栄養・食糧学会

日本神経科学学会

日本分子生物学会

日本農芸化学会

日本畜産学会

家畜栄養生理研究会