Morphological identification of Amphitetranychus speceis (Acari: Tetranychide) with crossbreeding, esterase zymograms and DNA barcode data　　　　　　　　　　　　　　　
Arabuli, T; M. W. Negm; T. Matsuda; Y. Kitashima; T. Abramishvili; I. A. Akimov; O. V. Zhovnerchuk; S. Ya. Popo; T. Gotoh
PLOS ONE, 2019年09月, ［査読有り］

ライトトラップのサイズおよび設置レイアウトがタバコシバンムシの捕獲効率に及ぼす影響
和栗伸伍・荻野翔真・北嶋康樹・後藤哲雄, 責任著者,

Light traps with sticky sheets are effective tools for controlling and monitoring stored-product pests. Cigarette beetle is an important storage pest. Light attracts both beetle sexes; often, single light traps are deployed in a large area, because they are larger and costlier than pheromone traps (which capture only males). Cigarette beetles occur in aggregates in areas with many obstacles, suggesting that a single trap is insufficient. To improve trapping efficacy at a low cost, we compared the number of trapped beetles among one, two or four small light traps (8 W) versus a single large light trap (50 W), attached to the wall(s) of a single darkroom (7.2×3.2×2.4 m, 25–30°C). Per treatment, 500 laboratory-reared adult beetles were released in the middle of the room. Half of the treatments had an opaque plastic wall (2×2 m) in the center of the room. After 6 hours, the mean number/trap of beetles captured with sticky sheets (7 replications) was 46.4, 39.4, 57.1 and 88.2, for one large and one, two and four small light traps, respectively. The obstacle did not affect these numbers. Hence, single lights trapped as many beetles, and more beetles were captured when more small lights were present.

, 日本衛生動物学会
衛生動物, 2016年06月, ［査読有り］

An LED-based UV-B irradiation system for tiny organisms: system description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa
Suzuki; T.; Y. Yoshioka; O. Tsarsitalidou; V. Ntalia; S. Ohno; K. Ohyama; Y. Kitashima; T. Gotoh; M. Takeda and D. S. Koveos, We developed a computer-based system for controlling the photoperiod and irradiance of UV-B and white light from a 5 x 5 light-emitting diode (LED) matrix (100 x 100 mm). In this system, the LED matrix was installed in each of four irradiation boxes and controlled by pulse-width modulators so that each box can independently emit UV-B and white light at irradiances of up to 1.5 and 4.0 W m(-2), respectively, or a combination of both light types. We used this system to examine the hatchabilities of the eggs of four Tetranychus spider mite species (T. urticae, T. kanzawai, T. piercei and T. okinawanus) collected from Okinawa Island under UV-B irradiation alone or simultaneous irradiation with white light for 12 h d(-1) at 25 degrees C. Although no eggs of any species hatched under the UV-B irradiation, even when the irradiance was as low as 0.02 W m(-2), the hatchabilities increased to >90% under simultaneous irradiation with 4.0 W m(-2) white light. At 0.06 W m(-2) UV-B, T. okinawanus eggs hatched (15% hatchability) under simultaneous irradiation with white light, whereas other species showed hatchabilities <1%. These results suggest that photolyases activated by white light may reduce UV-B-induced DNA damage in spider mite eggs and that the greater UV-B tolerance of T. okinawanus may explain its dominance on plants in seashore environments, which have a higher risk of exposure to reflected UV-B even on the undersurface of leaves. Our system will be useful for further examination of photophysiological responses of tiny organisms because of its ability to precisely control radiation conditions. (C) 2014 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
J. Insect Physiol., 2014年, ［査読有り］

Effect of hot-water treatment on the two-spotted spider mite, Tetranychus urticae, and its predator, Neoseiulus californicus (Acari: Tetranychidae, Phytoseiidae)
Gotoh; T.; Y. Kitashima and T. Sato, Hot-water treatment has been used on various kinds of plants to control plant diseases and pests. Here, we determined the lethal times (LT50 and LT100) for various temperatures for the two-spotted spider mite, Tetranychus urticae, and the predatory mite, Neoseiulus californicus (Spical®). Mites were placed on strawberry leaf discs that were dipped in hot water. For T. urticae eggs, LT50 ranged from 250 s at 47.5°C to 1 s at 57°C, while LT100 ranged from 600 s at 47.5°C to 7 s at 57°C. For T. urticae females, LT50 ranged from 380 s at 47.5°C to 4 s at 57°C, while LT100 ranged from 1200 s at 47.5°C to 9 s at 57°C. For N. californicus eggs, LT50 ranged from 68 s at 45°C to 7 s at 50°C, while LT100 ranged from 300 s at 45°C to 6 s at 52.5°C. For N. californicus females, LT50 ranged from 107 s at 45°C to 6 s at 50°C, while LT100 ranged from 180 s at 45°C to 6 s at 52.5°C. LT50 s for N. californicus eggs and females were 4-10 and 13-32 times shorter than those of T. urticae, respectively. The strawberry leaves did not appear to be damaged (i.e., they did not show significant wilting) when treated for 150 s at 50°C, 30 s at 55°C or 20 s at 57°C. Together, these results suggest that hot-water treatment can be used to control spider mites on strawberry, without much damage to the plants, but predatory mites may need to be replaced due to their sensitivity to hot water. © 2013 Taylor and Francis.
Internat. J. Acarol., 2013年, ［査読有り］

Susceptibility to acaricides in nine strains of the tomato red spider mite Tetranychus evansi (Acari: Tetranychidae)
Gotoh; T.; S. Fujiwara and Y. Kitashima, The tomato red mite, Tetranychus evansi Baker and Pritchard, is a herbivore specialized on solanaceous plants, which has rapidly spread through the world during the last decade. It causes serious damage to a variety of crops in Africa and Europe but has not yet been reported to damage solanaceous crops in Japan. Tetranychus evansi is known to occur in Japan and it has the potential to become a severe pest on solanaceous crops here as well. The present study determined the susceptibility of nine T. evansi strains from various countries to 11 acaricides, because chemicals will be used as the first line of control against a new pest when it starts to cause severe damage to crops. Bifenazate, cyenopyrafen, milbemectin, spirodiclofen and tebufenpyrad showed higher toxicity - as indicated by 50% lethal concentrations (LC50) - for imagocidal action, and chlorfenapyr showed higher LC50 for ovicidal action in almost all T. evansi strains, compared with a susceptible strain of Tetranychus kanzawai Kishida. Still, the LC50 of imagocidal action of all acaricides, and for ovicidal action of all but one (cyflumetofen), on all nine strains tested was much lower than the recommended concentrations. These results suggest that mites from all nine strains could be controlled with acaricides as a first method, should they occur in high numbers on crops., TAYLOR & FRANCIS INC
Internat. J. Acarol., 2011年, ［査読有り］

屋内と屋外に設置したライトトラップによる屋外昆虫の屋内への侵入数の評価　　　　　　　　　　　　　　　
守田詩穂; 田辺堅太郎; 田原雄一郎; 平尾 素一; 北嶋 康樹
応動昆, 2009年11月, ［査読有り］

Species composition of the spider mites (Acari: Tetranychidae) on vegetables in Okinawa, southwestern Japan
Ohno; S.; A. Miyagi; T. Ganaha-Kikumura; T. Gotoh; Y. Kitashima; T. Ooishi; T. Ando; K. Kijima; K. Futagami; T. Uesato and K. Yasuda, On the main islands of Japan and in Taiwan, the spider mites Tetranychus kanzawai and T urticae (green and red forms) are major pests on vegetable crops. The present study revealed that the dominant spider mite species injurious to vegetables in Okinawa, located between the main islands of Japan and Taiwan, differed from those in the two regions, providing important and fundamental information for controlling spider mites in Okinawa. T okinawanus was the most dominant species throughout Okinawa with the broadest host range, even though the species has been regarded as a non-pest to date. T piercei, which has similarly not been treated as a vegetable pest, was the second most dominant species with respect to frequency of occurrence and host range. T kanzawai and T urticae (green form) were comparable in the frequency of occurrence to T piercei in the northern part of Okinawa (Okinawa Islands), whereas they were scarce in the southern part (Sakishima Islands). As other less-dominant species, T ludeni, T neo-caledonicus and T pueraricola were found infesting several vegetable crops. T urticae (red form) was not found in this study., JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entomol. Zool., 2009年11月, ［査読有り］

A new species of the genus Bryobia Koch (Acari: Tetranychidae) from Iran
M. Khanjani; T. Gotoh; Y. Kitashima, A new mite, Bryobia mirmoayedii n. sp. (Tetranychidae), is described and illustrated based on females, a male and larvae collected from the grass, Poa bulbosa L. (Poaceae), in Rijabin Kermanshah Province, Iran., INDIRA PUBLISHING HOUSE
International Journal of Acarology, 2008年, ［査読有り］

A new species of the genus Bryobia Koch (Acari: Tetranychidae) from Iran　　　　　　　　　　　　　　　
M. Khanjani; T. Gotoh; Y. Kitashima
International Journal of Acarology, 2008年, ［査読有り］

タバコシバンムシの捕獲に及ぼすフェロモントラップの設置場所の影響　　　　　　　　　　　　　　　
北嶋康樹; 平尾素一; 高橋朋也
衛生動物, 2007年03月, ［査読有り］

Divergence in host range and reproductive compatibility in three strains of Oligonychus gotohi Ehara (Acari: Tetranychidae)
Tetsuo Gotoh; Satoshi Abe; Yasuki Kitashima; Shozo Ehara, Preliminary observations indicated that Oligonychus gotohi Ehara collected from deciduous chestnut (deciduous-tree strain) could not develop on leaves of the evergreen broad-leaved tree Lithocarpus edulis (Makino) Nakai in the laboratory. Furthermore, we found an evergreen arrhenotokous (A) strain that produces both female and male progeny and an evergreen thelytokous (T) strain that produces only female progeny. These observations suggest that O. gotohi consists of either host races or sibling species. To determine which is the case, we examined the above three strains on 19 fagaceous plant species. The deciduous-tree strain successfully developed and oviposited on seven deciduous trees but did not develop on evergreen trees at all. The evergreen-A strain had the narrowest host range and could develop and oviposit only on three species of deciduous trees and only on three species of evergreen trees. The evergreen-T strain grew well on six deciduous trees and on all but one of the evergreen trees. Thus, the three strains demonstrated host plant divergence. Malate dehydrogenase (MDH) zymograms among populations of the same strain were similar, but those from the different strains were clearly different. Inter-population crosses in the deciduous-tree and the evergreen-A strains were compatible, but the reciprocal crosses between the deciduous-tree and the evergreen-A strains produced no females, indicating that these two strains were reproductively isolated. Neither virgin females of the evergreen-T strain nor evergreen-T strain females crossed with males of either the deciduous-tree or the evergreen-A strain produced male offspring. Thus, O. gotohi contains at least three different biological entities that may be sibling species., INDIRA PUBLISHING HOUSE
Internat. J. Acarol. 33, 2007年, ［査読有り］

Wolbachia-induced cytoplasmic incompatibility in Japanese populations of Tetranychus urticae (Acari: Tetranychidae)
Tetsuo Gotoh; Jun Sugasawa; Hiroaki Noda; Yasuki Kitashima, Intracellular bacteria of the genus Wolbachia (alpha Proteobacteria) induce cytoplasmic incompatibility (CI) in many arthropod species, including spider mites, but not all Wolbachia cause CI. In spider mites CI becomes apparent by a reduced egg hatchability and a lower daughter:son ratio: CI in haplodiploid organisms in general was expected to produce all-male offspring or a male-biased sex ratio without any death of eggs. In a previous study of Japanese populations of Tetranychus urticae, two out of three green-form populations tested were infected with non-CI Wolbachia strains, whereas none of six red-form populations harbored Wolbachia. As the survey of Wolbachia infection in T. urticae is still fragmentary in Japan, we checked Wolbachia infection in thirty green-form populations and 29 red-form populations collected from a wide range of Japanese islands. For Wolbachia-infected populations, we tested the effects of Wolbachia on the reproductive traits and determined the phylogenetic relationships of the different strains of Wolbachia. All but one green-form populations were infected with Wolbachia and all strains belonged to the subgroup Ori when the wsp gene was used to determine the phylogenetic relationships of different strains of Wolbachia. Six out of 29 red-form populations harbored Wolbachia and the infected strains belonged to the subgroups Ori and Bugs. Twenty-four of 29 infected green-form populations and five of six infected red-form populations induced CI among the hosts. Thus, CI-Wolbachia strains are widespread in Japan, and no geographical trend was observed in the CI-Wolbachia. Although three red-form populations harbored other intracellular bacteria Cardinium, they did not affect host reproduction., SPRINGER
Exp. Appl. Acarol., 2007年, ［査読有り］

Tetranychus mite species identification using esterase and phosphoglucomutase zymograms
Tetsuo Gotoh; Yasuki Kitashima; Koichi Goka, Identification of spider mites based on morphological characteristics is difficult because the morphological differences are subtle and are usually specific to males, which are hard to find, and the adult stage. As an alternative method, we attempted to discriminate the 13 known Tetranychus species in Japan using only females, and esterase and phosphoglucomutase (PGM) zymograms. Using esterase zymograms we were able to discriminate females from five sympatric species (T ludeni, T kanzawai, T piercei, T pueraricola and T urticae (red form)) living on kudzu vine, Pueraria lobata, although a sixth sympatric species, T parakanzawai, was not distinguishable from the closely related T kanzawai. However, using PGM zymograms, females of each of the 13 species could be identified by the presence of one or two unique alleles. Thus, PGM isozyme analysis is a useful and simple tool for discriminating all Japanese species of the genus Tetranychus., JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entomol. Zool., 2007年, ［査読有り］

Compatibility between strains of Tetranychus pueraricola strains (Acari: Tetranychidae) correlated with distance between sites of their origin.
Akiyuki Suwa; Yasuki Kitashima; Tetsuo Gotoh, To test the hypothesis that the degree of incompatibility between strains of mites is correlated with the distance between the areas of their origin, seven local strains of Tetranychus pueraricola Ehara and Gotoh (Acari: Tetranychidae) were crossed with the Hitachi-ohta (Ho) and the Onoma (On) strains. For each F I generation obtained from crosses between the On strain and local strains, the number of eggs laid per female, egg hatchability, survival rate in immature stages, female ratio and average number of female offspring produced per female were similar to the values obtained in crosses of the On parent strain. Similar results were obtained for crosses between the Ho strain and all but one of the local strains. However, egg hatchability and average number of female offspring produced per female in the B I generation were less than those in the F-1 generation with large variations among cross combinations. The female ratio was significantly higher in the B I generation than in the F I generation. Thus, the F I generations obtained from crossings between any two strains were viable and fertile, but the degree of incompatibility in the B I generation varied from almost infertile to fertile. If compatibility in any one cross combination is estimated by "egg hatchability" and "female ratio", the compatibility in the B-1 generation was significantly correlated with the distance between the areas of their origin., INDIRA PUBLISHING HOUSE
Internat. J. Acarol., 2006年, ［査読有り］

Influence of prey on developmental performance, reproduction and prey consumption of Neoseiulus californicus (Acari: Phytoseiidae) Exp. Appl. Acarol.
Tetsuo Gotoh; Akiko Tsuchiya; Yasuki Kitashima, The Spical strain of the predatory mite Neoseiulus californicus (McGregor) is used as a biological control agent, but little is known about its preferred prey and host plants in Japan. Here we studied the development, reproduction and prey consumption of the Spical strain when fed on eggs of five different spider mite species deposited on both their laboratory-rearing plant and cherry, on which all five spider mite species developed well. The developmental periods of immature N. californicus females and males were significantly affected by the prey species they fed on, but not by the plants. No difference was found between males and females. The developmental period was shorter on eggs of two Tetranychus species than on eggs of Panonychus ulmi. Immature females had a higher predation rate than immature males. Preoviposition period, oviposition period and the number of eggs laid per female were not significantly affected by either the plants or the type of prey eggs. The postoviposition period and total adult longevity were shorter on eggs of P. ulmi than of the other four prey species, but there was no effect of plant substrate. The postoviposition period of the Spical strain was much longer than that of other N. californicus strains or other predatory mite species: the postoviposition period of the Spical strain was more than three times longer than the oviposition period, accounting for more than 75% of the total adult longevity. This suggests that the females need multiple mating to reach full egg load, but this remains to be tested. Total consumption by N. californicus adults was lower for eggs of P. ulmi than for eggs of the other four species, apparently because of the shorter postoviposition period when fed on eggs of P. ulmi. The intrinsic rates of natural increase (r (m)) on the rearing plant did not differ among prey species, whereas those on cherry were significantly different: the value was higher on Tetranychus urticae eggs than on eggs of other species. Only when N. californicus fed on T. urticae eggs, the r (m)-values were significantly different between the rearing plant and cherry (higher on cherry). Thus, the Spical strain of N. californicus could feed on eggs of all five spider mite species, deposited on a variety of plants with similar r (m)-values, suggesting that it could be successfully used to control spider mites in orchards and various crop fields of Japan., SPRINGER
Exp. Appl. Acarol., 2006年, ［査読有り］

Geographic variation of susceptibility to acaricides in two spider mite species, Panonychus osmanthi and P. citri (Acari: Tetranychidae) in Japan.
Tetsuo Gotoh; Yasuki Kitashima; Ishizue Adachi, The susceptibility of two Panonychus mite species to eight acaricides was examined. Many populations of P. osmanthi Ehara and Gotoh collected from Osmanthus plants and P. citri (McGregor) collected from either holly/yew or citrus were resistant to each of four acaricides registered before 1990, whereas almost all populations were susceptible to each of four new acaricides released on the market after 1990. Effectiveness of fenpyroximate in P. citri control was lower in central and southwestern areas of Japan than in the northern area, especially for populations infesting citrus trees. For all eight acaricides the average mortality of P. citri from citrus was lowest. Overall, little geographical variation in susceptibility was found in P. osmanthi, whereas some variation was detected for fenpyroximate in P. citri., INDIRA PUBLISHING HOUSE
Internat. J. Acarol., 2004年, ［査読有り］

Geographic variation of esterase and malate dehydrogenase allozymes in two spider mite species, Panonychus osmanthi and P. citri (Acari: Tetranychidae) in Japan.
Tetsuo Gotoh; Yasuki Kitashima Ishizue Adachi, The genetic basis of malate dehydrogenase (MDH) and an esterase (alpha-Est 3) in the citrus red mite Panonychus citri (McGregor) and P. osmanthi Ehara and Gotoh was studied by polyacrylamide gel electrophoresis and crossing experiments. Based on the banding patterns and the phenotype-segregation ratio, the MDH-1 appeared as a dimeric enzyme of a single locus composed of three codominant alleles (S, M and F) in P. citri. Esterase (alpha-Est 3), also a dimeric enzyme regulated by two codominant alleles (S and F), was polymorphic in some populations of P. citri, and the frequency of the F allele was higher in southwestern populations than in other populations. The F allele of P. osmanthi, a mite that superficially resembles P. citri, was observed in only one population and all others were fixed for the S allele. At the MDH-1 locus, all populations of P. osmanthi and P. citri were fixed for the M allele with one exceptional population of P. citri. In this population, most females exhibited a single band of the 'MM' homozygote, but one female showed three bands of the 'SM' heterozygote and two females showed three bands of the 'MF' heterozygote. Thus, little geographical variation was found in allele distribution at the MDH-1 locus, but some variation was detected at the alpha-Est 3 locus only in P. citri., INDIRA PUBLISHING HOUSE
Internat. J. Acarol., 2004年, ［査読有り］

Developmental and reproductive performance of Tetranychus pueraricole Ehara and Gotoh (Acari: Tetranychidae) at four constant temperatures.
Tetsuo Gotoh; Akiyuki Suwa; Yasuki Kitashima; H. A. Rezk, The spider mite Tetranychus pueraricola infests kudzu vines throughout Japan and causes conspicuous brownish feeding scars. It is possibility that T. pueraricola may get out of hand after invasion of agricultural leguminous crops. To better evaluate its potential severity as a pest, we determined the development, survivorship and life-history parameters of T. pueraricola on kidney bean. At temperatures between 15 and 30&DEG; C more than 89% of eggs hatched and of the newly hatched larvae ca. 89% attained maturity. There was no significant difference in eclosion rate of eggs or survival rate of immature stages among the temperatures. The lower threshold temperature from egg to oviposition was 10.9&DEG; C and the thermal constant was 147.1 degree-days. Based on these data, the maximum number of generations that can theoretically complete development under average field conditions in Ibaraki, Central Japan is between 11 and 14 generations per year. At 25&DEG; C, females laid on average 125 eggs during a mean oviposition period of 12.6 days. The intrinsic rate of natural increase (r(m)) was 0.102 at 15&DEG; C, 0.179 at 20&DEG; C, 0.299 at 25&DEG; C and 0.377 at 30&DEG; C. Thus, T pueraricola has the second highest r(m)-value among tetranychid mites reported so far at 25&DEG; C, with T .okinawanus having the highest (0.316). This indicates that T. pueraricola has the potential to become a serious pest on agricultural crops., JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entmol. Zool., 2004年, ［査読有り］

Development and oviposition of Tetranychus pueraricole Ehara and Gotoh (Acari: Tetranychidae) on various plants.　　　　　　　　　　　　　　　
Tetsuo Gotoh; Akiyuki Suwa; Yasuki Kitashima
J. Acarol. Soc. Jpn., 2004年, ［査読有り］

Population dynamics of Panonychus osmanthi (Acari: Tetranychidae) on two Osmanthus species
北嶋康樹・後藤哲雄, Panonychus osmanthi is a non-diapausing species of spider mite that superficially resembles P. citri. It infests Osmanthus species, which are evergreen roadside and garden trees. The population dynamics of P. osmanthi were studied on Osmanthus aurantiacus and O. x fortunei during a three-year period. Seasonal changes in P. osmanthi populations were fundamentally the same in each year, although their density differed greatly from year to year. The P. osmanthi population was bimodal, with one peak in spring ( May - June) and another in winter ( November - January). Populations abruptly declined after the spring peak. Predators showed a delayed density-dependent response to changes in spider mites from spring to summer, whereas in autumn and winter, predators were few because they had entered diapause. To determine the effect of predators on the rapid decline of spider mites just after the spring peak, the predators were removed by treating the trees with a synthetic pyrethroid. As a result, spider mite density did not decline after the spring peak and remained at a high level during the June-August period when spider mite density is usually very low. This suggests that predators play an important role in the drastic decline of P. osmanthi density just after the spring peak., KLUWER ACADEMIC PUBL
Exp. Appl. Acarol., 2003年, ［査読有り］

Life-history traits of the six Panonychus species from Japan (Acari: Tetranychidae).
Tetsuo Gotoh; Yukio Ishikawa; Yasuki Kitashima, Six species of the genus Panonychus are known from Japan. Life-history parameters of all six species were investigated at 25 S C, and for three species two strains of different geographical origin were included. The intrinsic rate of natural increase (r(m)) ranged from 0.172/ day for the P. osmanthi albino strain to 0.209/day for P. citri, while the net reproductive rate (R-0) varied from 23.98 in the thelytokous species P. thelytokus to 46.61 in P. citri. Both values were higher in the polyphagous species ( P. ulmi, P. mori and P. citri), which are considered crop pests, than those in the oligophagous species ( P. thelytokus and P. osmanthi), considered non-pests. The only exception was P. bambusicola, an oligophagous non-pest species with R-0- and r(m)- values closely resembling those of the three polyphagous species., KLUWER ACADEMIC PUBL
Exp. Appl. Acarol., 2003年, ［査読有り］

Hybrid affinities between the green and the red forms of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae) under laboratory and semi-natural conditions.
Jun Sugasawa; Yasuki Kitashima; Tetsuo Gotoh, The systematic status of the green and the red forms of Tetranychus urticae Koch is still unresolved because the ability of the two forms to interbreed varies considerably depending on the populations used. First, to investigate the hybrid fertility between the two forms in the laboratory, we interbred them for five generations. The hybrids produced showed relatively lower fitness (lower hatchability, lower proportion of female adults and lower production of fertile offspring) than their parents, and in some crossings, no female offspring emerged. Second, we used phosphoglucoisomerase (PGI) allozymes as genetic markers to analyze the temporal changes in hybridization of the two forms under semi-natural conditions. The temporal changes of hybridization were analyzed by the F-statistic. Intra-form mating was initially predominant, but as the number of damaged leaves increased, inter-form mating increased because the mites dispersed more frequently. However, as the fitness of hybrids from inter-form mating was much lower than that from intra-form mating, most hybrids were unable to produce offspring or had become extinct on the rose leaves. Thus, the gene flow between the two forms appears to be extremely restricted, indicating that a strong genetic differentiation is present between the two forms., JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entomol. Zool., 2002年, ［査読有り］

Susceptibility of the red spider mite, Oligonychus coffeae (Acari: Tetranychidae), to acaricides on tea plants in Japan.　　　　　　　　　　　　　　　
Tetsuo Gotoh; Yasuki Kitashima; Koichi Goka; Toru Nagata
Internat. J. Acarol., 2001年, ［査読有り］

Electrophoretic differentiation of F1 hybrids of the sibling species Panonychus osmanthi Ehara et Gotoh and P. citri (McGregor) (Acari: Tetranychidae).
Yasuki Kitashima; Tetsuo Gotoh, JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entomol. Zool., 1997年, ［査読有り］

Genetic control of albinism in Panonychus osmanthi Ehara et Gotoh (Acari: Tetranychidae).
Yasuki Kitashima; Tetsuo Gotoh, Three albino females were discovered in a field population of Panonychus osmanthi, the albinism being a result of spontaneous mutation. To determine the genetic control of the albinism, standard genetic crosses were conducted. Apparently the albinism was recessive to the normal pigmented trait and was under monogenic control. Albino mites with reddish eye-spots were obtained when pigmented hybrid females obtained from crosses between the albino and normal strains were either kept virgin or mated to males with various phenotypes. The eye-spots of albino females were not passed on to the next generation irrespective of the male phenotype. These results suggested that some pigment precursor possessed by pigmented females is responsible for the appearance of the eye-spots. Hybrid females resulted in a reduction in hatchability in F-2 eggs and a distortion of the female : male ratio. These phenomena might be explained in terms of the difference in mortality between haploid and diploid eggs: i.e., crosses between hybrid females and pure albino males without eye-spots resulted in the death of about 50% of both haploid and diploid eggs, whereas crosses between hybrid females and either normal males or eye-spotted albino males resulted in the death of about 50% of haploid eggs but did not cause the death of diploid eggs., JAPAN SOC APPL ENTOMOL ZOOL
Appl. Entomol. Zool., 1997年, ［査読有り］

Host range difference and reproductive incompatibility among five populations of the citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae)
Yasuki Kitashima; Tetsuo Gotoh
J. Acarol. Soc. Jpn., 1995年11月, ［査読有り］

食品工場における新しい防虫・防鼠管理システム. 最終章 システム全体のチェックおよび総括
北嶋康樹,高橋朋也
HACCP, 2005年02月

食品工場における新しい防虫・防鼠管理システム. 第4章 ネズミ害虫のIPM的防除
北嶋康樹,高橋朋也
HACCP, 2005年01月

食品工場における新しい防虫・防鼠管理システム. 第3章 防虫活動をスムーズに行うための組織のあり方と定期チェックの手法
北嶋康樹,高橋朋也
HACCP, 2004年12月

食品工場における新しい防虫・防鼠管理システム. 第2章 工場における防虫上の問題点に関する実態把握の手法
北嶋康樹,高橋朋也
HACCP, 2004年11月

食品工場における新しい防虫・防鼠管理システム. 第1章 防虫・防鼠管理の必要性と考え方
北嶋康樹,高橋朋也
HACCP, 2004年10月

ミカンハダニに近縁なモクセイハダニの生態と防除への利用
北嶋康樹
今月の農業, 2000年

黄色蛍光灯および遮光カーテンの走行性昆虫の誘引抑制効果の検証
野口優香・小林優希・北嶋康樹
第67回日本応用動物昆虫学会大会, 2023年03月15日, 日本応用動物昆虫学会
20230313, 20230315

紫外線カット蛍光灯の昆虫誘引抑制効果の検証
北嶋康樹・廣菜々子
第67回日本応用動物昆虫学会大会, 2023年03月15日, 日本応用動物昆虫学会
20230313, 20230315

ハンノキアケハダニのハンノキ寄生系統およびケヤマハンノキ寄生系統における分類学的検討
阿部初紀・蓮本勇太・北嶋康樹
第67回日本応用動物昆虫学会大会, 2023年03月14日, 日本応用動物昆虫学会
20230313, 20230315

Tetranychus kanzawai Kishida (Acari: Tetranychidae)　　　　　　　　　　　　　　　
Amini, M. Y; A. Osawa; Y. Takano; T. Suzuki; Y. Kitashima; T. Gotoh
第24回日本ダニ学会大会, 2015年09月13日, 日本ダニ学会

無酸素処理によ る選択的ハダニ防除技術の開発　　　　　　　　　　　　　　　
王 至弘・鈴木丈詞・北嶋康樹・後藤哲雄
第23回日本ダニ学会大会, 2014年10月19日, 日本ダニ学会

温度周期がスミスアケハダニの休眠誘導に及ぼす影響　　　　　　　　　　　　　　　
高野友二郎・鈴木丈詞・北嶋康樹・後藤哲雄
第23回日本ダニ学会大会, 2014年10月18日, 日本ダニ学会

無酸素処理による害虫防除および天敵分離技術の開発　　　　　　　　　　　　　　　
王 至弘・鈴木丈詞・北嶋康樹・後藤哲雄
第58回日本応用動物昆虫学会大会, 2014年03月28日, 日本応用動物昆虫学会

同所的近縁２種（Neoseiulus longispinosusおよびケナガカブリダニ）の分布を制限している要因は何か　　　　　　　　　　　　　　　
菅原令大・北嶋康樹・何 琦琛・後藤哲雄
第58回日本応用動物昆虫学会大会, 2014年03月28日, 日本応用動物昆虫学会

スミスアケハダニの温度依存性休眠機構の解明　　　　　　　　　　　　　　　
高野友二郎・鈴木丈詞・北嶋康樹・後藤哲雄
第58回日本応用動物昆虫学会大会, 2014年03月28日, 日本応用動物昆虫学会

沖縄産ハダニ４種の卵におけるUV-B損傷と光回復の種間差　　　　　　　　　　　　　　　
吉岡義雄・鈴木丈詞・北嶋康樹・後藤哲雄・大野 豪
第58回日本応用動物昆虫学会大会, 2014年03月28日, 日本応用動物昆虫学会

屋外の環境を室内に再現する変動環境シミュレータの開発：第２号試作機　　　　　　　　　　　　　　　
鈴木丈詞・高野友二郎・北嶋康樹・後藤哲雄・平井規央・渋谷俊夫・大山克己・天野 洋
第58回日本応用動物昆虫学会大会, 2014年03月28日, 日本応用動物昆虫学会

世界のクダハダニ属は２種か、それとも３種か　　　　　　　　　　　　　　　
後藤哲雄・松田朋子・北嶋康樹・I. A. Akimov・S. Ya. Popov
第 58回日本応用動物昆虫学会大会, 2014年03月27日, 日本応用動物昆虫学会

Tetranychus属ハダニにおけるDNA塩基配列による系統関係の推定　　　　　　　　　　　　　　　
福本千尋・日本典秀・北嶋康樹・後藤哲雄
第54回日本応用動物昆虫学会大会, 2010年03月27日

Oligonychus属ハダニの形態およびDNA塩基配列による系統解析.　　　　　　　　　　　　　　　
松田朋子・日本典秀・北嶋康樹・後藤哲雄
第54回日本応用動物昆虫学会大会, 2010年03月27日

Preliminary survey of spider mite fauna in Bangladesh　　　　　　　　　　　　　　　
Haque; M. A.・Y. Kitashima・M. S. Ullah・T. Gotoh
第54回日本応用動物昆虫学会大会, 2010年03月27日

温湯処理によるイチゴのナミハダニ防除　　　　　　　　　　　　　　　
亀里慶永・北嶋康樹・佐藤達雄・後藤哲雄
第54回日本応用動物昆虫学会大会, 2010年03月26日

ミツユビナミハダニとTetranychus evansiの関係(予報)　　　　　　　　　　　　　　　
新木涼志・北嶋康樹・後藤哲雄
第52回日本応用動物昆虫学会大会、宇都宮大学, 2008年03月28日, 日本応用動物昆虫学会

ナミハダニ黄緑型の雄の日齢と受精能力との関係　　　　　　　　　　　　　　　
森田晃将・北嶋康樹・後藤哲雄
第52回日本応用動物昆虫学会大会、宇都宮大学, 2008年03月28日

Does borage have any effect on spider mite predators?　　　　　　　　　　　　　　　
El-Shafefi, G.M.A; Y. Kitashima; T. Gotoh
第52回日本応用動物昆虫学会大会、宇都宮大学, 2008年03月28日

ミカンハダニに極めて近縁なモクセイマルハダニの発見：寄主転換と種分化　　　　　　　　　　　　　　　
北嶋康樹
第52回日本応用動物昆虫学会大会小集会（ハダニ談話会）、宇都宮大学, 2008年03月28日

カンザワハダニと近縁な“アジサイナミハダニ”の存在：形態は語る　　　　　　　　　　　　　　　
後藤哲雄・北嶋康樹・M. H. Badii
第52回日本応用動物昆虫学会大会、宇都宮大学, 2008年03月26日

チビコブツメハダニおよびツバキに寄生するOligonychus sp. の生活史パラメータ　　　　　　　　　　　　　　　
北嶋康樹・井本一弘・後藤哲雄
第16回日本ダニ学会, 2007年10月26日

ナミハダニの野外個体群における交尾雌率と雌雄比　　　　　　　　　　　　　　　
森田晃将・北嶋康樹・後藤哲雄
第16回日本ダニ学会大会、千葉大学, 2007年10月26日

Performance of seventeen tetranychid mite species on borage and strawberry　　　　　　　　　　　　　　　
El-Shafei, G.M.A; Kitashima, Y; Gotoh, T
. 第16回日本ダニ学会大会、千葉大学, 2007年10月26日

タイ産カンザワハダニの生殖特性　　　　　　　　　　　　　　　
山田総一郎・後藤哲雄・北嶋康樹
第51回日本応用動物昆虫学会大会、広島大学, 2007年03月29日

ナミハダニは第2雄の精子を使うか　　　　　　　　　　　　　　　
森田晃将・北嶋康樹・後藤哲雄
第51回日本応用動物昆虫学会大会、広島大学, 2007年03月29日

食品害虫フェロモントラップの設置条件が捕獲数に及ぼす要因　　　　　　　　　　　　　　　
北嶋康樹・平尾素一
第51回日本応用動物昆虫学会大会, 2007年03月27日

非休眠種であるモクセイマルハダニの越冬戦略　　　　　　　　　　　　　　　
北嶋康樹・後藤哲雄
第15回日本ダニ学会大会, 2006年10月27日

Overwintering tactics in the non- diapausing species, Panonychus osmanthi.　　　　　　　　　　　　　　　
Kitashima, Y; T. Gotoh
12th International Congress of Acarology. Amsterdam, The Netherlands, 2006年08月21日

モクセイマルハダニのナシ園への侵入の可能性とミカンハダニとの交尾干渉.　　　　　　　　　　　　　　　
北嶋康樹・後藤哲雄
第11回農林害虫防除研究会, 2006年06月23日

日本応用動物昆虫学会

日本ダニ学会

日本衛生動物学会

日本ペストロジー学会

日本家屋害虫学会

新農薬実用化試験に関する研究　　　　　　　　　　　　　　　
茨城大学
2022年02月 - 2023年03月

新農薬による各種害虫の生態と防除に関する研究　　　　　　　　　　　　　　　
新農薬による各種害虫の生態と防除に関する研究
2021年04月 - 2022年12月