Yoichi NAKAHIRAAssociate Professor
■Researcher basic information
Organization
Research Areas
Research Keyword
Educational Background
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(Message from Researchers)
(教員からのメッセージ) 植物に特異的な細胞小器官である「葉緑体」や、葉緑体と進化的類縁関係にある「ラン藻」に興味をもって研究を進めています。大学院生・研究員時代は、葉緑体DNAにコードされた遺伝子の発現制御（とりわけ転写制御）に関する解析や、ラン藻の概日時計の分子機構の解明など、基礎生物学的な研究が中心でした。その中で巡り合った解析手法の１つである「葉緑体ゲノムへの遺伝子導入技術 =【葉緑体工学】」は、基礎研究において有効であるのみならず、食料・医療・エネルギー・環境など、さまざまな産業分野に貢献できる『 次世代型遺伝子組換え作物 』の創出においても威力を発揮します。茨大農学部における研究では、【葉緑体工学】を活用することにより、持続可能な社会の形成に資する有用遺伝子組換え植物を開発し、その実用化を目指します。
■Research activity information
Paper
- 〔Major achievements〕Novel bovine viral diarrhea virus (BVDV) virus-like particle vaccine candidates presenting the E2 protein using the SpyTag/SpyCatcher system induce a robust neutralizing antibody response in mice
Miki Katsura; Masaki Fukushima; Ken-Ichiro Kameyama; Takehiko Kokuho; Yoichi Nakahira; Kaoru Takeuchi
Archives of Virology, 07 Jan. 2023, [Reviewed] - 〔Major achievements〕Lack of plastid‐encoded Ycf10, a homolog of the nuclear‐encoded DLDG1 and the cyanobacterial PxcA, enhances the induction of non‐photochemical quenching in tobacco
Mai Duy Luu Trinh; Akira Hashimoto; Masaru Kono; Shinichi Takaichi; Yoichi Nakahira; Shinji Masuda, Wiley
Plant Direct, 14 Dec. 2021, [Reviewed] - 〔Major achievements〕Mass Production of Virus-Like Particles Using Chloroplast Genetic Engineering for Highly Immunogenic Oral Vaccine Against Fish Disease.
Yoichi Nakahira; Kaori Mizuno; Hirofumi Yamashita; Minami Tsuchikura; Kaoru Takeuchi; Takashi Shiina; and Hidemasa Kawakami, Lead, Nervous necrosis virus (NNV) is the causative agent of viral nervous necrosis (VNN), which is one of the most serious fish diseases leading to mass mortality in a wide range of fish species worldwide. Although a few injectable inactivated vaccines are commercially available, there is a need for more labor-saving, cost-effective, and fish-friendly immunization methods. The use of transgenic plants expressing pathogen-derived recombinant antigens as edible vaccines is an ideal way to meet these requirements. In this study, chloroplast genetic engineering was successfully utilized to overexpress the red-spotted grouper NNV capsid protein (RGNNV-CP). The RGNNV-CP accumulated at high levels in all young, mature, and old senescent leaves of transplastomic tobacco plants (averaging approximately 3 mg/g leaf fresh weight). The RGNNV-CP efficiently self-assembled into virus-like particles (RGNNV-VLPs) in the chloroplast stroma of the transgenic lines, which could be readily observed by in situ transmission electron microscopy. Furthermore, intraperitoneal injection and oral administration of the crudely purified protein extract containing chloroplast-derived RGNNV-VLPs provided the sevenband grouper fish with sufficient protection against RGNNV challenge, and its immunogenicity was comparable to that of a commercial injectable vaccine. These findings indicate that chloroplast-derived VLP vaccines may play a promising role in the prevention of various diseases, not only in fish but also in other animals, including humans., frontiers
frontiers in Plant Science, 23 Aug. 2021, [Reviewed] - 〔Major achievements〕Selective activation of chloroplast psbD light-responsive promoter and psaA/B promoter in transplastomic tobacco plants overexpressing Arabidopsis sigma factor AtSIG5.
Nozoe M; Tsunoyama Y; Ishizaki Y; Nakahira Y; Shiina T., BACKGROUND: Plastid-encoded eubacterial-type RNA polymerase (PEP) plays a critical role in the transcription of photosynthesis genes in chloroplasts. Notably, some of the reaction center genes, including psaA, psaB, psbA, and psbD genes, are differentially transcribed by PEP in mature chloroplasts. However, the molecular mechanism of promoter selection in the reaction center gene transcription by PEP is not well understood. OBJECTIVE: Sigma factor proteins direct promoter selection by a core PEP in chloroplasts as well as bacteria. AtSIG5 is a unique chloroplast sigma factor essential for psbD light-responsive promoter (psbD LRP) activity. To analyze the role of AtSIG5 in chloroplast transcription in more detail, we assessed the effect of AtSIG5 hyper-expression on the transcription of plastid-encoded genes in chloroplast transgenic plants. RESULTS: The chloroplast transgenic tobacco (CpOX-AtSIG5) accumulates AtSIG5 protein at extremely high levels in chloroplasts. Due to the extremely high-level expression of recombinant AtSIG5, most PEP holoenzymes are most likely to include the recombinant AtSIG5 in the CpOXAtSIG5 chloroplasts. Thus, we can assess the promoter preference of AtSIG5 in vivo. The overexpression of AtSIG5 significantly increased the expression of psbD LRP transcripts encoding PSII reaction center D2 protein and psaA/B operon transcripts encoding PSI core proteins. Furthermore, run-on transcription analyses revealed that AtSIG5 preferentially recognizes the psaA/B promoter, as well as the psbD LRP. Moreover, we found that psbD LRP is constitutively active in CpOX-AtSIG5 plants irrespective of light and dark. CONCLUSION: AtSIG5 probably plays a significant role in differential transcription of reaction center genes in mature chloroplasts.
Protein Pept Lett., 14 Oct. 2019, [Reviewed] - 〔Major achievements〕A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import.
Kikuchi S; Asakura Y; Imai M; Nakahira Y; Kotani Y; Hashiguchi Y; Nakai Y; Takafuji K; Bédard J; Hirabayashi-Ishioka Y; Mori H; Shiina T; Nakai M., Chloroplasts import thousands of nucleus-encoded preproteins synthesized in the cytosol through the TOC and TIC translocons on the outer and inner envelope membranes, respectively. Preprotein translocation across the inner membrane requires ATP; however, the import motor has remained unclear. Here, we report that a 2-MD heteromeric AAA-ATPase complex associates with the TIC complex and functions as the import motor, directly interacting with various translocating preproteins. This 2-MD complex consists of a protein encoded by the previously enigmatic chloroplast gene ycf2 and five related nuclear-encoded FtsH-like proteins, namely, FtsHi1, FtsHi2, FtsHi4, FtsHi5, and FtsH12. These components are each essential for plant viability and retain the AAA-type ATPase domain, but only FtsH12 contains the zinc binding active site generally conserved among FtsH-type metalloproteases. Furthermore, even the FtsH12 zinc binding site is dispensable for its essential function. Phylogenetic analyses suggest that all AAA-type members of the Ycf2/FtsHi complex including Ycf2 evolved from the chloroplast-encoded membrane-bound AAA-protease FtsH of the ancestral endosymbiont. The Ycf2/FtsHi complex also contains an NAD-malate dehydrogenase, a proposed key enzyme for ATP production in chloroplasts in darkness or in nonphotosynthetic plastids. These findings advance our understanding of this ATP-driven protein translocation system that is unique to the green lineage of photosynthetic eukaryotes.
Plant Cell, 11 Oct. 2018, [Reviewed] - 〔Major achievements〕Comparative Analysis of Chloroplast psbD Promoters in Terrestrial Plants
Shuichi Shimmura; Mikio Nozoe; Shota Kitora; Satoko Kin; Shigeru Matsutani; Yoko Ishizaki; Yoichi Nakahira; Takashi Shiina
FRONTIERS IN PLANT SCIENCE, Jul. 2017, [Reviewed] - 〔Major achievements〕Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria
Sayaka Hondo; Masatoshi Takahashi; Takashi Osanai; Mami Matsuda; Tomohisa Hasunuma; Akio Tazuke; Yoichi Nakahira; Shigeru Chohnan; Morifumi Hasegawa; Munehiko Asayama
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Nov. 2015, [Reviewed] - Diversity in Guanosine 3 ',5 '-Bisdiphosphate (ppGpp) Sensitivity among Guanylate Kinases of Bacteria and Plants
Yuhta Nomura; Atsushi Izumi; Yoshinori Fukunaga; Kensuke Kusumi; Koh Iba; Seiya Watanabe; Yoichi Nakahira; Andreas P. M. Weber; Akira Nozawa; Yuzuru Tozawa
JOURNAL OF BIOLOGICAL CHEMISTRY, May 2014, [Reviewed] - 〔Major achievements〕Theophylline-dependent riboswitch as a novel genetic tool for strict regulation of protein expression in cyanobacterium synechococcus elongatus PCC 7942
Yoichi Nakahira; Atsushi Ogawa; Hiroyuki Asano; Tokitaka Oyama; Yuzuru Tozawa, Lead
Plant and Cell Physiology, Oct. 2013, [Reviewed] - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Yusuke Nakai; Yoichi Nakahira; Hiroki Sumida; Kosuke Takebayashi; Yumiko Nagasawa; Kanako Yamasaki; Masako Akiyama; Masaru Ohme-Takagi; Sumire Fujiwara; Takashi Shiina; Nobutaka Mitsuda; Eiichiro Fukusaki; Yasuyuki Kubo; Masa H. Sato
Plant Journal, Mar. 2013, [Reviewed] - Erratum: Chloroplast-mediated activation of plant immune signalling in Arabidopsis (Nature Communications (2012) 3 (926) DOI: 10.1038/ncomms1926)
Hironari Nomura; Teiko Komori; Shuhei Uemura; Yui Kanda; Koji Shimotani; Kana Nakai; Takuya Furuichi; Kohsuke Takebayashi; Takanori Sugimoto; Satoshi Sano; I. Nengah Suwastika; Eiichiro Fukusaki; Hirofumi Yoshioka; Yoichi Nakahira; Takashi Shiina
Nature Communications, 2013, [Reviewed] - 〔Major achievements〕Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering.
Nakahira Y; Ishikawa K; Tanaka K; Tozawa Y; and Shiina T, Lead
Biosci. Biotechnol. Biochem., 2013, [Reviewed] - Chloroplast-mediated activation of plant immune signalling in Arabidopsis
Hironari Nomura; Teiko Komori; Shuhei Uemura; Yui Kanda; Koji Shimotani; Kana Nakai; Takuya Furuichi; Kohsuke Takebayashi; Takanori Sugimoto; Satoshi Sano; I. Nengah Suwastika; Eiichiro Fukusaki; Hirofumi Yoshioka; Yoichi Nakahira; Takashi Shiina
NATURE COMMUNICATIONS, Jun. 2012, [Reviewed] - Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase
Yusuke Yagi; Yoko Ishizaki; Yoichi Nakahira; Yuzuru Tozawa; Takashi Shiina
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 2012, [Reviewed] - Function and evolution of plastid sigma factors.
Shiina T; Ishizaki Y; Yagi Y; and Nakahira Y, Last
Plant Biotechnol., 2009, [Reviewed] - Evidence for chloroplast control of external Ca2+-induced cytosolic Ca2+ transients and stomatal closure.
Nomura H; Komori T; Kobori M; Nakahira Y; and Shiina T
Plant J., 2008, [Reviewed] - Regulation of external calcium-induced stomatal closure by chloroplast localized CAS
Nomura Hironari; Komori Teiko; Nakahira Yoichi; Shiina Takashi
PLANT AND CELL PHYSIOLOGY, 2007, [Reviewed] - Coordinated functions of plastid sigma factors in chloroplast development and gene expression
Shiina T; Ishizaki Y; Ozono K; Takenaka C; Hanaoka M; Kanamaru K; Tanaka K; Nakahira Y
Photosynthesis Research, 2007, [Reviewed] - [Plastid transformation in higher plants: application for chloroplast factory].
Nakahira Y; Shiina T
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Nov. 2005, [Reviewed] - Characterization of a plastid sigma factor AtSIG5 responsible for psbDlight-responsive transcription.
Nozoe M; Ishizaki Y; Tsunoyama Y; Tsubokura Y; Kato K; Shinnmyo A; Nakahira Y; Shiina T
"Photosynthesis: Fundamental Aspects to Global Perspectives ”A. van der Est, D. Bruce, eds., Kluwer Academic Publishers, Dordrecht., 2005, [Reviewed] - A nuclear-encoded sigma factor, Arabidopsis SIG6, recognizes sigma-70 type chloroplast promoters and regulates early chloroplast development in cotyledons.
Ishizaki Y; Tsunoyama Y; Hatano K; Ando K; Kato K; Shinmyo A; Kobori M; Takeba G; Nakahira Y; and Shiina T
Plant J., 2005, [Reviewed] - Transcriptional regulation of the circadian clock operon kaiBC by upstream regions in cyanobacteria.
Kutsuna S; Nakahira Y; Katayama M; Ishiura M; and Kondo T, In the cyanobacterium, Synechococcus elongatus PCC 7942, the kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate circadian oscillation. We investigated the regulation of kaiBC transcription. A primer extension and deletion analyses of the upstream region mapped the sufficient promoter region (SPR) to base pairs -55 to +1 (the transcription start site, TSS) and identified a constitutive negative regulatory region upstream of the SPR (base pairs -897 to -56) that extended into the coding sequence of kaiA. Base-pair substitution within the SPR identified a sequence from -52 to -28 that was the essential element for transcription. Most of the examined sequences drove rhythmic expression of a luxAB reporter that was similar to the expression driven by the kaiBC promoter (PkaiBC) and responded to the overexpression of kaiA or kaiC, even in a promoter activity range of 1-8000%. These results indicate that circadian feedback regulation by KaiA and KaiC is addressed to a global step preceding transcription driven by PkaiBC. However, increasing or decreasing the intrinsic activity of PkaiBC greatly affected the rhythm, suggesting that constitutive adjustment of PkaiBC activity by the sequences identified here is essential for the oscillator.
Mol. Microbiol., 2005, [Reviewed] - 〔Major achievements〕Global gene repression by KaiC as a master process of prokaryotic circadian system.
Nakahira Y; Katayama M; Miyashita H; Kutsuna S; Iwasaki H; Oyama T; and Kondo T, Lead
Proc. Natl. Acad. Sci. USA, 2004, [Reviewed] - An in vivo dual-reporter system of cyanobacteria using two railroad-worm luciferases with different color emissions.
Kitayama Y; Kondo T; Nakahira Y; Nishimura H; Ohmiya Y; and Oyama T
Plant Cell Physiol., 2004, [Reviewed] - Blue light-induced transcription of plastid-encoded psbD gene is mediated by a nuclear-encoded transcription initiation factor, AtSig5.
Tsunoyama Y; Ishizaki Y; Morikawa K; Kobori M; Nakahira Y; Takeba G; Toyoshima Y; and Shiina T
Proc. Natl. Acad. Sci. USA, 2004, [Reviewed] - 〔Major achievements〕Mutations in KaiA, a clock protein, extend the period of circadian rhythm in the cyanobacterium Synechococcus elongatus PCC 7942.
Nishimura H; Nakahira Y; Imai K; Tsuruhara A; Kondo H; Hayashi H; Hirai M; Saito H; and Kondo T, Lead
Microbiology, 2002, [Reviewed] - シアノバクテリアにおけるkai時計遺伝子作用モデルの再検討
岩崎 秀雄; 中平 洋一; 片山 光徳; 近藤 孝男
日本時間生物学会会誌: Journal of Chronobiology, 01 Oct. 2001 - Circadian-regulated expression of a nuclear-encoded plastid sigma factor gene (sigA) in wheat seedlings.
Morikawa K; Ito S; Tsunoyama Y; Nakahira Y; Shiina T; and Toyoshima Y
FEBS Lett., 1999, [Reviewed] - Developmental stage-specific multi-subunit plastid RNA polymerases (PEP) in wheat.
Satoh J; Baba K; Nakahira Y; Tsunoyama Y; Shiina T; and Toyoshima Y
Plant J., 1999, [Reviewed] - 〔Major achievements〕Circadian-regulated transcription of the psbD light-responsive promoter in wheat chloroplasts seedlings.
Nakahira Y; Baba K; Yoneda A; Shiina T; and Toyoshima Y, Lead
Plant Physiol., 1998, [Reviewed] - 〔Major achievements〕Circadian-regulated transcription of the psbD light-responsive promoter (psbD LRP) in wheat chloroplasts.
Nakahira Y; Morikawa K; Shiina T; and Toyoshima Y, Lead
In Photosynthesis: Mechanisms and Effects (Garab, G eds.) Kluwer Academic publishers, 1998 - Characterization of dynamics of the psbD light-induced transcription in mature wheat chloroplasts.
Satoh J; Baba K; Nakahira Y; Shiina T; and Toyoshima Y
Plant Mol. Biol., 1997, [Reviewed] - Cis and trans factor(s) of a light-responsive promoter of psbD/C gene cluster in wheat.
Tsunoyama Y; Nakahira Y; Shiina T; and Toyoshima Y
"Photosynthesis: from Light to Biosphere" edited by P. Mathis Kluwer Academic Publishers, 1995 - Possible roles of light-responsive psb promoters in regulation of turnover of PSII proteins.
Baba K; Satoh J; Nakahira Y; Shiina T; and Toyoshima Y
"Photosynthesis: from Light to Biosphere" edited by P. Mathis Kluwer Academic Publishers, 1995
MISC
- 〔Major achievements〕葉緑体工学でつくる水産用ワクチン植物
中平 洋一
アグリバイオ, Nov. 2024, [Invited]
Lead - 〔Major achievements〕葉緑体工学によるモノづくり,高等植物葉緑体ゲノム改変技術の進展とその応用
中平 洋一
化学と生物, 01 Mar. 2024, [Reviewed], [Invited]
Lead - Chloroplast Ca2+ binding protein CAS and salicylic acid biosynthesis
Shiina Takashi; Yoshioka Hirofumi; Fukusaki Eiichiro; Uemura Shihei; Kanda Yui; Sano Satoshi; Takebayashi Kosuke; Sugimoto Takanori; Bamba Takeshi; Nakahira Yoichi; Nomura Hironari
Plant and Cell Physiology Supplement, 2011 - NAC-like transcription factor,VOZは生物学的ストレスと非生物学的ストレスに対する適応制御の切り換えに関与する。
中井勇介; 中平洋一; 隅田浩規; 安居佑季子; 河内孝之; 高木優; 光田展隆; 久保康之; 佐藤雅彦
日本植物学会大会研究発表記録, 2011 - シロイヌナズナNAC関連転写因子VOZは非生物学的ストレス応答の制御に関与している
中井勇介; 中平洋一; 安居佑季子; 河内孝之; 椎名隆; 高木優; 光田展隆; 佐藤雅彦
日本植物生理学会年会要旨集, 2011 - フィトクロムシグナル伝達において機能する花成促進因子VOZの局在解析
安居佑季子; 西谷亜依子; 上本充大; 硯亮太; 向川佳子; 中井勇介; 中平洋一; 佐藤雅彦; 河内孝之
日本植物生理学会年会要旨集, 12 Mar. 2010 - Involvement of a chloroplast-localized protein CAS in the external Ca2+-induced stomatal closure.
H Nomura; M Kobori; Y Tsunoyama; E Iwagishi; Y Nakahira; T Shiina
PLANT AND CELL PHYSIOLOGY, 2006 - An Arabidopsis double mutant sig2sig6 exhibits albino phenotype.
Y Ishizaki; K Ozono; C Takenaka; Y Tsunoyama; Y Nakahira; K Tanaka; K Kanamaru; M Hanaoka; T Shiina
PLANT AND CELL PHYSIOLOGY, 2006 - Characterization of a specific plastid sigma factor AtSIG5 using chloroplast transformation
M Nozoe; Y Ishizaki; Y Tsunoyama; Y Tsubokura; Y Nakahira; T Shiina
PLANT AND CELL PHYSIOLOGY, 2005 - 〔Major achievements〕Plastid transcription in higher plants.
Toyoshima Y; Onda Y; Shiina T; and Nakahira Y
Critical Reviews in Plant Sciences, 2005
Last - 〔Major achievements〕Plastid RNA polymerases, promoters and transcription regulators in higher plants.
Shiina T; Tsunoyama Y; Nakahira Y; and Khan MS
International Review of Cytology, 2005 - Arabidopsis Sig6 is a general sigma factor in chloroplasts, important for transcription of photosynthesis-related genes at an early stage of seedling growth
Y Ishizaki; Y Tsunoyama; K Hatano; M Kobori; G Takeba; Y Nakahira; T Shiina
PLANT AND CELL PHYSIOLOGY, 2004 - Monitoring of circadian gene expression by rail-road worm luciferases dual-reporter system
Y Kitayama; T Oyama; Y Nakahira; Viviani, V; Y Ohmiya; T Kondo
PLANT AND CELL PHYSIOLOGY, 2003 - 〔Major achievements〕Transcriptional regulation of the kaiABC clock genes in cyanobacteria
Y Nakahira; M Katayama; H Iwasaki; T Kondo
PLANT AND CELL PHYSIOLOGY, 2002 - 〔Major achievements〕Light-induced switching of promoter recognition specificity of chloroplast polymerase.
T Shiina; K Baba; Y Tsunoyama; Y Nakahira; A Yoneda; K Morikawa; Y Toyoshima
PLANT PHYSIOLOGY, Jul. 1997 - 〔Major achievements〕葉緑体における転写制御機構
中平 洋一; 豊島 喜則
日本農芸化学会誌, 1997
Lead
Lectures, oral presentations, etc.
- 〔Major achievements〕高活性アルカン生合成酵素を用いたシアノバクテリアにおける持続可能な航空燃料（SAF）の生産性向上
石田梨紗子; 金子太樹; 中平洋一
第66回 日本植物生理学会年会（金沢）, 15 Mar. 2025 - 〔Major achievements〕ポジティブ・ネガティブ選抜マーカーを併用した新規「マーカーフリー葉緑体形質転換植物作出法」の開発
小薗江彩唯; 石川奈緒美; 松村優斗; 桑田小夜子; 中平洋一
第66回 日本植物生理学会年会（金沢）, 14 Mar. 2025 - 〔Major achievements〕Systematic modification of the theophylline-dependent synthetic riboswitches for cyanobacteria
藤原 未来; 大嶋 真由子; 中平 洋一
第41回 日本植物バイオテクノロジー学会（仙台）大会, 01 Sep. 2024 - 〔Major achievements〕Transplastomic tobacco plants overexpressing thermostable cellulases
坂本 晴那; 磯野 真秀; 中平 洋一
第41回 日本植物バイオテクノロジー学会（仙台）大会, 31 Aug. 2024 - 〔Major achievements〕Modification of theophylline-dependent synthetic riboswitch for production of useful substances in cyanobacteria.
藤原 未来、 大嶋 真由子、 中平 洋一
第65回 日本植物生理学会年会（神戸）, 17 Mar. 2024 - 〔Major achievements〕魚病防除用ウイルス様粒子（VLP）ワクチンを大量発現する葉緑体形質転換レタス
平山 美羽、原川 翔伍、川上 秀昌、中平 洋一
第40回 日本植物バイオテクノロジー 学会（千葉）大会, 12 Sep. 2023 - マロニル-CoA生合成を強化した大腸菌での遊離脂肪酸生産
濵田 美志、中平 洋一、西澤 智康、長南 茂
第74回日本生物工学会大会, 20 Oct. 2022 - 〔Major achievements〕Generation of an Oral Vaccine for Aquaculture Fish Using Chloroplast Engineering
中平 洋一
第38回日本植物バイオテクノロジー学会（つくば）大会,シンポジウム 「植物オルガネラゲノム工学の新展開」, 10 Sep. 2021, [Invited] - 〔Major achievements〕茨城大など、タバコ葉で魚病ウイルスの経口ワクチン開発 大量生産でコストダウン、今後は食用植物で実用化目指す
日経バイオテクONLINE, 31 Aug. 2021, [Invited] - 〔Major achievements〕養殖魚に経口ワクチン 茨城大学など開発 疾病予防に効果
日本経済新聞（北関東地域面）, 26 Aug. 2021, [Invited] - 〔Major achievements〕養殖魚に経口ワクチン 愛媛県農水研など開発 疾病予防に
日本経済新聞（四国版）, 26 Aug. 2021, [Invited] - 〔Major achievements〕植物で魚病ワクチン作出 茨城大など研究チーム開発 経口投与で免疫効果確認
日刊水産経済新聞, 24 Aug. 2021, [Invited] - 〔Major achievements〕養魚に｢食べるワクチン植物｣ ウイルス疾病予防に有効 既存注射型と同等の高免疫原性
みなと新聞, 24 Aug. 2021, [Invited] - 〔Major achievements〕Development of a strictly controlled inducible gene expression system for plastids by using synthetic riboswitches
RIKA YAMANE; ATSUSHI OGAWA; YUZURU TOZAWA; TAKASHI SHIINA; and YOICHI NAKAHIRA
The 2019 Gordon Research Conference on Chloroplast Biotechnology, 08 Jan. 2019 - 〔Major achievements〕Overproduction of a virus-like-particle (VLP) vaccine against a fish disease by plastid engineering
Yoichi Nakahira; Hirofumi Yamashita; Minami Tsuchikura; Kaoru Takeuchi; and Takashi Shiina
The 2019 Gordon Research Conference on Chloroplast Biotechnology, 07 Jan. 2019 - 〔Major achievements〕Improvement of the synthetic riboswitch-based inducible gene expression system in plastids
RIKA YAMANE; ATSUSHI OGAWA; YUZURU TOZAWA; TAKASHI SHIINA; and YOICHI NAKAHIRA
第41回日本分子生物学会年会, 28 Nov. 2018 - 〔Major achievements〕葉緑体工学を用いた水産用VLPワクチンの大量生産
中平 洋一; 山下 浩史; 土倉 みなみ; 竹内 薫; 椎名 隆
第36回日本植物細胞分子生物学会（金沢）大会, 28 Aug. 2018 - 〔Major achievements〕EVALUATION OF THEOPHYLLINE-DEPENDENT SYNTHETIC RIBOSWITCHES FOR STRICT CONTROL OF PLASTID GENE EXPRESSION
RIKA YAMANE; ATSUSHI OGAWA; YUZURU TOZAWA; TAKASHI SHIINA; and YOICHI NAKAHIRA
International Plant Molecular Biology 2018, 07 Aug. 2018 - 〔Major achievements〕HIGH-LEVEL PRODUCTION OF A VIRUS-LIKE PARTICLE VACCINE AGAINST A FISH NERVOUS NECROSIS VIRUS IN TRANSPLASTOMIC TOBACCO PLANTS
YOICHI NAKAHIRA; HIROFUMI YAMASHITA; MINAMI TSUCHIKURA; KAORU TAKEUCHI; and TAKASHI SHIINA
International Plant Molecular Biology 2018, 06 Aug. 2018 - 〔Major achievements〕Utilization of the theophylline-dependent engineered riboswitch for improved alkane production in cyanobacteria
金子 太樹; 福田 寛史; 武波 洋志; 朝山 宗彦; 中平 洋一
2017年度 生命科学系学会合同年次大会, 07 Dec. 2017 - 〔Major achievements〕Evaluation of the synthetic riboswitch-based inducible gene expression system in plastids
山根 里佳; 小川 敦司; 戸澤 譲; 椎名 隆; 中平 洋一
2017年度 生命科学系学会合同年次大会, 07 Dec. 2017 - 〔Major achievements〕人工リボスイッチを活用した葉緑体遺伝子発現制御系の評価
山根 里佳; 小川 敦司; 戸澤 譲; 椎名 隆; 中平 洋一
第35回 日本植物細胞分子生物学会（さいたま）大会, 29 Aug. 2017 - 〔Major achievements〕Metabolic engineering for improved production of drop-in fuels in the cyanobacterium Synechococcus elongatus PCC 7942
Hiroki Kaneko; Hirofumi Fukuda; MunehikoAsayama; Yoichi Nakahira
第58回 日本植物生理学会年会, 16 Mar. 2017 - 〔Major achievements〕Utilization of a theophylline-dependent engineered riboswitch for enhanced alkane production in the cyanobacterium Synechocystis sp. PCC 6803
Hirofumi Fukuda; Hiroki Kaneko; MunehikoAsayama; Yoichi Nakahira
第58回 日本植物生理学会年会, 16 Mar. 2017 - 〔Major achievements〕シアノバクテリアにおけるドロップイン燃料生産性強化に向けた遺伝子改変技術の開発
金子太樹; 山根里佳; 櫻井大貴; 福田寛史; 朝山宗彦; 中平洋一
第39回 日本分子生物学会年会, 02 Dec. 2016 - 〔Major achievements〕シアノバクテリアのアルカンと脂肪生産に与える栄養源欠乏培地の影響
福田寛史; 中平洋一; 長谷川守文; 宮口右二; 朝山宗彦
第39回 日本分子生物学会年会, 02 Dec. 2016 - 〔Major achievements〕人工リボスイッチを基盤とした葉緑体遺伝子発現誘導系
中平洋一; 山根里佳; 小川敦司; 戸澤譲; 椎名隆
第39回 日本分子生物学会年会, 01 Dec. 2016 - 〔Major achievements〕人工リボスイッチを用いたタバコ葉緑体遺伝子発現誘導系
中平洋一; 小川敦司; 戸澤譲; 椎名隆
第34回 日本植物細胞分子生物学会（上田）大会, 01 Sep. 2016 - 〔Major achievements〕Genetic engineering for enhanced alkane production in the model cyanobacterium Synechococcus elongatus PCC 7942
金子太樹; 福田寛史; 朝山宗彦; 中平洋一
第57回日本植物生理学会年会, 20 Mar. 2016 - 〔Major achievements〕Utilization of the theophylline-dependent engineered riboswitches for strict control of plastid gene expression in tobacco.
中平 洋一; 小川 敦司; 戸澤 譲; 椎名 隆
第57回日本植物生理学会年会, 18 Mar. 2016 - Genetic Engineering and Overproduction of Polyhydroxybutyrate in Cyanobacteria
Sayaka Hondo; Masatoshi Takahashi; Takashi Osanai; Mami Matsuda; Tomohisa Hasunuma; Akio Tazuke; Yoichi Nakahira; Shigeru Chonan; Morifumi Hasegawa; and Munehiko Asayama
THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, Dec. 2015 - 藻類バイオプラスチックPHB高生産株の創生と特徴付け
本堂彩花; 高橋正俊; 小山内崇; 松田真実; 蓮沼誠久; 長谷川守文; 中平洋一; 朝山宗彦
第３７回 日本分子生物学会年会, 27 Nov. 2014 - 〔Major achievements〕人工リボスイッチを基盤としたラン藻の新規遺伝子発現制御技術
中平 洋一; 小川 敦司; 浅野 宏幸; 小山 時隆; 戸澤 譲
第55回 日本植物生理学会年会, 20 Mar. 2014 - 〔Major achievements〕人工リボスイッチを用いたラン藻のための新規遺伝子発現制御技術
中平 洋一; 小川 敦司; 浅野 宏幸; 小山 時隆; 戸澤 譲
第36回 日本分子生物学会年会, Dec. 2013 - 〔Major achievements〕Overproduction of Thermostable Biomass-Saccharizing Enzymes in Tobacco Chloroplasts: Towards Developing the Self-Saccharizing Crops
Nakahira Y; Kashima Y; Tozawa Y; and Shiina T
3rd International Symposium on Chloroplast Genomics and Genetic Engineering, May 2013 - 〔Major achievements〕ラン藻のための新規遺伝子発現制御系の開発
中平 洋一; 小川 敦司; 浅野 宏幸; 小山 時隆; 戸澤 譲
第54回 日本植物生理学会年会, Mar. 2013 - 〔Major achievements〕葉緑体工学による有用植物の開発
中平 洋一; 鹿島 康浩; 椎名 隆; 戸澤 譲
日本農芸化学会中四国支部大会（第34回講演会）, Sep. 2012 - 〔Major achievements〕葉緑体工学による自己糖化型タバコの作出
第29回日本植物細胞分子生物学会大会, Sep. 2011 - 〔Major achievements〕High-Level Expression of a Set of Six Thermostable Cell Wall-Degrading Enzymes in Tobacco Chloroplasts: A First Step Towards Development of the Auto-Saccharification System of Bioenergy Crops.
22nd International Conference on Arabidopsis Research, May 2011 - 〔Major achievements〕葉緑体工学による耐熱性糖化酵素群の大量発現
中平 洋一; 鹿島 康浩
日本農芸化学会2011年度大会, Mar. 2011 - 〔Major achievements〕超耐熱性セルラーゼを大量発現する葉緑体形質転換植物
中平 洋一; 田中 國介; 石川 一彦; 椎名 隆
日本農芸化学会2009年度大会, Mar. 2009 - 〔Major achievements〕葉緑体工学による超耐熱性セルラーゼの大量発現
中平 洋一; 田中 國介; 石川 一彦; 椎名 隆
第50回 日本植物生理学会年会, Mar. 2009 - 〔Major achievements〕葉緑体工学を用いた超耐熱性セルラーゼの大量発現
第31回 日本分子生物学会年会・第81回 日本生化学会大会 合同大会, Dec. 2008 - 〔Major achievements〕プラスチド核様体に関連するSWIBドメイン蛋白質の解析
中平 洋一; 矢田 和正; 椎名 隆
第47回 日本植物生理学会年会, Mar. 2006 - 〔Major achievements〕Firefly luciferase as a vital reporter for gene expression in tobacco chloroplasts.
Nakahira Y; Takeba G; Shiina T
XIIIth International Congress on Photosynthesis, Aug. 2004 - 〔Major achievements〕ルシフェラーゼを利用した葉緑体の遺伝子発現モニター系
中平 洋一; 野添 幹雄; 竹場 剛; 椎名 隆
第45回 日本植物生理学会年会, Mar. 2004 - 〔Major achievements〕ルシフェラーゼ遺伝子を用いた葉緑体遺伝子発現モニタ系の開発
中平 洋一; 野添 幹雄; 椎名 隆
第26回 日本分子生物学会年会, Dec. 2003
Affiliated academic society
Research Themes
- 〔Major achievements〕バイオジェット燃料を“一気通貫生産”するエネルギー作物の開発
Apr. 2023 - Mar. 2024 - 〔Major achievements〕さまざまな魚病の防除に適用可能な「水産用ワクチン植物」創出基盤技術の開発
トライアウト
Oct. 2022 - Mar. 2024 - 〔Major achievements〕タンパク質“自己結合”システムを用いた多機能型ウイルス様中空粒子ワクチンの開発
Grant-in-Aid for Scientific Research (C)
University of Tsukuba
Apr. 2020 - Mar. 2023 - 〔Major achievements〕“低コスト”・“省力”・“高免疫原性”を兼ね備えた「水産用ワクチン植物」の開発
研究成果最適展開支援プログラム(A-STEP) 機能検証フェーズ
Nov. 2019 - Mar. 2021 - 〔Major achievements〕医食同源により万病予防を実現できる「経口ワクチン植物」創製基盤技術の開発
挑戦的研究(萌芽)
Jun. 2018 - Mar. 2021 - 〔Major achievements〕完全人工合成された葉緑体ゲノムを有する遺伝子組換え植物の創出
Grant-in-Aid for Exploratory Research
Apr. 2015 - Mar. 2018 - Novel mechanisms of the cellular integration through plastid-nuclear communications in plant cells
Grant-in-Aid for Scientific Research (B)
Kyoto Prefectural University
01 Apr. 2013 - 31 Mar. 2017 - 〔Major achievements〕葉緑体工学を用いた養殖魚のための疾病予防植物の創出
Nov. 2012 - Oct. 2013 - 〔Major achievements〕葉緑体工学を用いた自己糖化型エネルギー作物の開発
Oct. 2008 - Mar. 2011 - Calcium signal transduction network in plant innate immunity
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
Kyoto Prefectural University
2008 - 2010
