1998, Award for encouragement of research from The Phytopathological Society of Japan

エンドファイトがイチゴの花芽形成を誘導する　　　　　　　　　　　　　　　
成澤才彦
植物の生長調節, Dec. 2024, [Invited]

ここまでわかった植物と微生物の関係　　　　　　　　　　　　　　　
成澤才彦, Lead
植物の生長調節, Dec. 2024, [Invited]

Isolation, structural elucidation, and biological activity of a novel isocoumarin from the dark septate endophytic fungus Phialocephala fortinii.
Kei Bando; Ryoga Kushibe; Naoki Kitaoka; Yutaka Tamai; Kazuhiko Narisawa and Hideyuki Matsuura, A novel isocoumarin was isolated from the mycelia of the dark septate endophytic fungus Phialocephala fortinii. The chemical structure was determined to be 8-hydroxy-6-methoxy-3,7-dimethyl-1H-2-benzopyran-1-one based on mass spectrometry, 1H-nuclear magnetic resonance (NMR), and 13C-NMR spectroscopic analyses, including 2D-NMR experiments. The isolated compound inhibited root growth of Arabidopsis thaliana, suggesting its potential as a plant growth regulator.
Zeitschrift für Naturforschung C, Mar. 2024, [Reviewed]

Accumulation of aluminium and arsenic inCenococcum geophilum sclerotia from forest soilaffected by mining smoke.
Khulan Nyamsanjaa; Akira Genseki; Tomohiro Hatano; Bolormaa Oyuntsetseg; Kazuhiko Narisawa and Makiko Watanabe., Informa UK Limited
Chemistry and Ecology, 18 Jan. 2024, [Reviewed]

Dark septate endophytic fungi associated with pioneer grass inhabiting volcanic deposits and their functions in promoting plant growth.
Han Sun; Tomoyasu Nishizawa; Hiroyuki Ohta; and Kazuhiko Narisawa, Corresponding
Biogeosciences, Nov. 2023, [Reviewed]

Detection and isolation of a new member of Burkholderiaceae‑related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales.
Yusuke Takashima; Kohei Yamamoto; Yousuke Degawa; Yong Guo; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Corresponding, Abstract

Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island in the Ogasawara (Bonin) Islands, Japan. The fungus was morphologically, phylogenetically, and physiologically characterized and proposed as a new species, Saksenaea boninensis sp. nov. Besides the fungal taxonomy, we also found the presence of BRE in isolates of this species by diagnostic PCR amplification of the 16S rRNA gene from mycelia, fluorescence microscopic observations, and isolation of the bacterium in pure culture. Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. The discovery of a culturable BRE lineage in the genus Saksenaea will add new insight into the evolutional origin of mucoralean fungus-BRE associations and emphasize the need to pay more attention to endofungal bacteria potentially associated with isolates of thermotolerant mucoralean fungi causing mucormycosis., Springer Science and Business Media LLC
IMA Fungus, Nov. 2023, [Reviewed]

31 駒止湿原開墾跡地における土着微生物を用いたブナ植樹試験 −植樹後5年の生育経過−（関東支部講演会）
轟 丈瑠; 郭 永; 野口 愛; 西澤 智康; 成澤 才彦; 坂上 伸生, 一般社団法人 日本土壌肥料学会
日本土壌肥料学会講演要旨集, 04 Sep. 2023

〔Major achievements〕First Report of Tripartite Symbiosis Potential among Soybean, Bradyrhizobium japonicum, and Dark Septate Endophytes.
Ni Luh Putu Citra Innosensia; I Putu Wirya Suputra; Gusti Ngurah Alit Susanta Wirya and Kazuhiko Narisawa., Corresponding
Agronomy, Jun. 2023, [Reviewed]

Investigating Endobacteria that Thrive Within Mucoromycota.
Alessandro Desirò; Yusuke Takashima; Gregory Bonito; Tomoyasu Nishizawa; Kazuhiko Narisawa; Paola Bonfante, Metagenomics approaches have revealed the importance of Mucoromycota in the evolution and functioning of plant microbiomes. Comprised of three subphyla (Glomeromycotina, Mortierellomycotina, and Mucoromycotina), this early diverging lineage of fungi encompasses species of mycorrhizal fungi, root endophytes, plant pathogens, and many decomposers of plant debris. Interestingly, several taxa of Mucoromycota share a common feature, that is, the presence of endobacteria within their mycelia and spores. The study of these endosymbiotic bacteria is still a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, a deeper understanding of such microbial interactions is now possible and fuels an emerging research field. In this chapter, we report how Mucoromycota, in particular Mortierellomycotina, and their endobacteria can be investigated using a combination of diverse cellular biology, microscopy, and molecular techniques.
Methods in molecular biology (Clifton, N.J.), 2023

The Dark Septate Endophyte, Phialocephala fortiniiJ2PC4, Mitigating Southern Rice Black-Streaked Dwarf Disease and Lethal to White Back Planthopper.
YanZhang; BixiaQin; LingXie; KazuhikoNarisawa; QianNong; LipingQin
Biological Control, Apr. 2022, [Reviewed]

Revisiting the isolation source after the first discovery: Myconymphaea yatsukahoi on excrements of Lithobiomorpha (Chilopoda)
Yusuke Takashima; Mai Suyama; Kohei Yamamoto; Tomohiko Ri; Kazuhiko Narisawa; Yousuke Degawa, Myconymphaea yatsukahoi is a fungus that has only been isolated once from a forest in the Sugadaira Research Station, Nagano, Japan. Over 20 y have passed since its first discovery but since then it has not been rediscovered. Here, we re-isolated M. yatsukahoi from the type locality and another location, Tambara Moor, Gunma, Japan. Sporophores of this species were detected by direct field observation in Sugadaira and by induction from soil from Tambara. We attempted to narrow down isolation sources of this species by investigating the excrements of Lithobiomorpha and Scolopendromorpha centipedes, which are frequently found in the two locations where the species is distributed. In both locations, we found M. yatsukahoi in the excrements of Lithobiomorpha but not Scolopendromorpha. Myconymphaea yatsukahoi appears to be a coprophilous fungus and the excrements of the predators living in soil may be promising isolation sources for understanding the hidden diversity of kickxellalean fungi., The Mycological Society of Japan
Mycoscience, Apr. 2022, [Reviewed]

見えてきた？菌類と細菌の協働作業
成澤 才彦, 公益社団法人 日本生物工学会
生物工学会誌, 25 Feb. 2022

〔Major achievements〕Biocontrol of Pythium root rot on lisianthus using a new dark septate endophytic fungus Hyaloscypha variabilis J1PC1.
Malek Marian; Yusuke Takashima; Wiwiek Harsonowati; Haruhiko Murota; Kazuhiko Narisawa, Last, Springer
European Journal of Plant Pathology, Feb. 2022, [Reviewed]

Whole-genome sequence of Entomortierella parvispora E1425, a Mucoromycotan fungus associated with Burkholderiaceae-related endosymbiotic bacteria.
Afri Herlambang; Yong Guo; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta; Tomoyasu Nishizawa, Some mucoromycotan fungi establish symbiotic associations with endohyphal bacteria. Here, the genome of
Entomortierella parvispora
E1425 (synonymously known as
Mortierella parvispora
E1425), which harbors a cultured
Burkholderiaceae
-related endobacterium (BRE) designated
Mycoavidus
sp. Strain B2-EB, was sequenced. We provide genomic information to elucidate fungal-BRE symbiotic features., American Society for Microbiology
Microbiology Resource Announcements, 13 Jan. 2022, [Reviewed]

〔Major achievements〕Prospecting the unpredicted potential traits of Cladophialophora chaetospira SK51 to alter photoperiodic flowering in strawberry, a perennial SD plant.
1)W. Harsonowati; Masrukhin; K. Narisawa, Corresponding
Scientia Horticulturae, Dec. 2021, [Reviewed]

Synergistic and Offset Effects of Fungal Species Combinations on Plant Performance.
Hori Y; H. Fujita; K. Hiruma; K. Narisawa and H. Toju, frontiers
Front. Microbiol., 13 Sep. 2021, [Reviewed]

The cellulolytic activity and symbiotic potential of dark septate endophytic fungus Phialocephala fortinii to promote non-mycorrhizal plants growth
Surono; K. Narisawa, The endophytic fungi have attracted much attention to investigating their ability to degrade cellulose and promote plant growth as double functional roles. The objective of this study was to investigate the cellulase activity and the ability of dark septate endophytic fungus (DSE) Phialocephala fortinii to promote non-mycorrhizal plants. This study consisted of carboxy-methylcellulose degradation activity of DSE isolates, cellulase activity, and the effect of cellulolytic DSE to promote non-mycorrhizal plants growth assays. The results showed P. fortinii III.Pi.I8 had the largest cellulolytic index than P. fortinii CKG.II.10.1 and P. eryngii for 7 days of incubation time. The cellulase activity pattern of all P. fortinii isolates was rather similar to P. eryngii for the incubation time of 7 to 35 days. Phialocephala fortinii III.Pi.I8, CKG.II.10.1, and CKG.I.11 increased the growth of Brassica oleracea var. capitata by 7.63, 92.5, and 47.7%, and Spinacia oleracea by 58.5, 283, and 303%, respectively, over to the control plant. Heavy colonization of P. fortinii occurred both in B. oleracea var. capitata and S. oleracea roots. This study proved that although P. fortinii has cellulase activity but did not cause disease symptoms in non-mycorrhizal plants so that it can potentially be multifunctional as cellulose decomposer and plant growth promoter., IOP Publishing Ltd
IOP Conference Series: Earth and Environmental Science, 22 Feb. 2021

アブラナ科植物はどのように栽培するのが良いのか? : その生理,生態,品質や環境への影響を考える (第21回大会) -- (全体セッション2)
成澤 才彦, 日本有機農業学会
有機農業研究 = Japanese journal of organic agriculture science, 2021

〔Major achievements〕Mycoavidus sp. Strain B2-EB: Comparative Genomics Reveals Minimal Genomic Features Required by a Cultivable Burkholderiaceae-Related Endofungal Bacterium.
Y. Guo; Y. Takashima; Y. Sato; K. Narisawa; H. Ohta; T. Nishizawa., Obligate bacterial endosymbionts are critical to the existence of many eukaryotes. Such endobacteria are usually characterized by reduced genomes and metabolic dependence on the host, which may cause difficulty in isolating them in pure cultures. Family Burkholderiaceae-related endofungal bacteria affiliated with the Mycoavidus-Glomeribacter clade can be associated with the fungal subphyla Mortierellomycotina and Glomeromycotina. In this study, a cultivable endosymbiotic bacterium, Mycoavidus sp. B2-EB, present in the fungal host Mortierella parvispora, was obtained successfully. The B2-EB genome (1.88 Mb) represents the smallest genome among the endofungal bacteria Mycoavidus cysteinexigens (2.64–2.80 Mb) of Mortierella elongata and uncultured endosymbionts ‘Candidatus Glomeribacter gigasporarum’ (1.37–2.36 Mb) of arbuscular mycorrhizal fungi. Despite a reduction in genome size, strain B2-EB displays a high genome completeness suggesting a nondegenerative reduction in the B2-EB genome. Compared with a large proportion of transposable elements (TEs) in other known Mycoavidus genomes (7.2–11.5% of total genome length), TEs accounted for only 2.4% of the B2-EB genome. This pattern, together with a high proportion of single-copy genes in the B2-EB genome, suggests that the B2-EB genome reached a state of relative evolutionary stability. These results represented the most streamlined structure among the cultivable endofungal bacteria and suggested the minimal genome features required by both an endofungal lifestyle and artificial culture. This study allowed us to understand the genome evolution of Burkholderiaceae-related endosymbionts and to elucidate microbiological interactions.


Importance This study attempts the isolation of a novel endobacterium, Mycoavidus sp. B2-EB (= JCM 33615), harbored in the fungal host Mortierella parvispora E1425 (= JCM 39028). We report the complete genome sequence of this strain, which possesses a reduced genome size with relatively high genome completeness and a streamlined genome structure. The information indicates the minimal genomic features required by both endofungal lifestyle and artificial cultivation, which furthers our understanding of genome reduction in fungal endosymbionts and extends the culture resources for biotechnological development on engineering synthetic microbiomes., American Society for Microbiology
Applied and Environmental Microbiology, 01 Sep. 2020, [Reviewed]

〔Major achievements〕The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities.
W. Harsonowati; M. Marian; K. Narisawa., Last
Front. Microbiol, 15 Apr. 2020, [Reviewed]

〔Major achievements〕Aposymbiosis of a Burkholderiaceae-Related Endobacterium Impacts on Sexual Reproduction of Its Fungal Host.　　　　　　　　　　　　　　　
Takashima Y.; Degawa Y.; Nishizawa T.; Ohta H.; Narisawa K., Last
Microbes and Environments, 15 Apr. 2020, [Reviewed]

Mortierella oedorhiza, a new species forming a dichotomously branched rhizoid at the sporangiophore base.
Takashima Y.; Degawa Y.; Narisawa K., Corresponding
Mycoscience, 2019, [Reviewed]

Publisher Correction: Core microbiomes for sustainable agroecosystems.
Hirokazu Toju; Kabir G Peay; Masato Yamamichi; Kazuhiko Narisawa; Kei Hiruma; Ken Naito; Shinji Fukuda; Masayuki Ushio; Shinji Nakaoka; Yusuke Onoda; Kentaro Yoshida; Klaus Schlaeppi; Yang Bai; Ryo Sugiura; Yasunori Ichihashi; Kiwamu Minamisawa; E Toby Kiers, Owing to a technical error, this Perspective was originally published without its received and accepted dates; the dates "Received: 31 December 2017; Accepted: 23 March 2018" have now been included in all versions.
Nature plants, Sep. 2018

Mortierella sugadairana, a new homothallic species related to the firstly described heterothallic species in the genus
Yusuke Takashima; Yousuke Degawa; Hiroyuki Ohta; Kazuhiko Narisawa, A new species, Mortierella sugadairana, is described for a fungus forming homothallic zygospores with a club-shaped macrosuspensor and a microsuspensor originating from the macrosuspensor. The species was isolated from cool regions in Japan and morphologically and phylogenetically close to a heterothallic species M. parvispora, which is the first species reported as a heterothallic species in the genus. Mycelial growth of the species was limited at 30 °C, whereas two isolates of M. parvispora can grow. This may indicate that the species and M. parvispora adapted to different climates from a common ancestor involving differentiation of the manner of reproduction., Elsevier B.V.
Mycoscience, 01 May 2018, [Reviewed], [Invited]

Effects of Rhizobium Species Living with the Dark Septate Endophytic Fungus Veronaeopsis simplex on Organic Substrate Utilization by the Host
Guo Yong; Matsuoka Yuuto; Nishizawa Tomoyasu; Ohta Hiroyuki; Narisawa Kazuhiko, Corresponding,

Bacteria harbored in/on the hyphae of the dark septate endophyte, Veronaeopsis simplex Y34, were identified as a single Rhizobium species by molecular analyses of bacterial 16S rRNA genes, and were successfully isolated from the endophyte. The Rhizobium-cured fungus was prepared thoroughly by an antibiotic treatment, thereby allowing an examination of their effects on organic substrate utilization. Assays with Biolog^® FF microplates revealed that the respiration potential for 52.6% of the tested compounds were significantly different between Rhizobium-harboring and -cured fungal hosts, indicating that organic substrate utilization by V. simplex Y34 was significantly influenced by the presence of the associated Rhizobium sp. VsBac-Y9.

, 日本微生物生態学会・日本土壌微生物学会
Ｍｉｃｒｏｂｅｓ ａｎｄ ｅｎｖｉｒｏｎｍｅｎｔｓ, 2018

Comparative Genomic Insights into Endofungal Lifestyles of Two Bacterial Endosymbionts, Mycoavidus cysteinexigens and Burkholderia rhizoxinica
Dilruba Sharmin; Yong Guo; Tomoyasu Nishizawa; Shoko Ohshima; Yoshinori Sato; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta, Japanese Society of Microbial Ecology
Microbes and Environments, 2018, [Reviewed]

The inhibitory role of dark septate endophytic fungus Phialocephala fortinii against Fusarium disease on the Asparagus officinalis growth in organic source conditions.　　　　　　　　　　　　　　　
Surono; Narisawa K., Corresponding
Biological Control, 2018, [Reviewed]

Core microbiomes for sustainable agroecosystems.
1)Hirokazu Toju; Kabir G. Peay; Masato Yamamichi; Kazuhiko Narisawa; Kei Hiruma; Ken Naito; Shinji Fukuda; Masayuki Ushio; Shinji Nakaoka; Yusuke Onoda; Kentaro Yoshida; Klaus Schlaeppi; Yang Bai; Ryo Sugiura; Yasunori Ichihashi; Kiwamu Minamisawa and E. Toby Kiers., In an era of ecosystem degradation and climate change, maximizing microbial functions in agroecosystems has become a prerequisite for the future of global agriculture. However, managing species-rich communities of plant-associated microbiomes remains a major challenge. Here, we propose interdisciplinary research strategies to optimize microbiome functions in agroecosystems. Informatics now allows us to identify members and characteristics of 'core microbiomes', which may be deployed to organize otherwise uncontrollable dynamics of resident microbiomes. Integration of microfluidics, robotics and machine learning provides novel ways to capitalize on core microbiomes for increasing resource-efficiency and stress-resistance of agroecosystems.
Nature Plants, 2018, [Reviewed]

Complete genome sequence of Agrobacterium pusense VsBac-Y9, a bacterial symbiont of the dark septate endophytic fungus Veronaeopsis simplex Y34 with potential for improving fungal colonization in roots　　　　　　　　　　　　　　　
1)Y. Guo; Y. Matsuoka; T. Miura; T. Nishizawa; H. Ohta; K. Narisawa, Corresponding
Journal of Biotechnology, 2018, [Reviewed]

Fungus-bacterium symbionts promote plant health and performance.　　　　　　　　　　　　　　　
Y. Guo; K. Narisawa., Corresponding
Microbes and Environments, 2018, [Invited]

Prevalence and Intra-Family Phylogenetic Divergence of Burkholderiaceae-Related Endobacteria Associated with Species of Mortierella.
Takashima Y.; Seto K.; Degawa Y.; Guo Y.; Nishizawa T.; Ohta H.; Narisawa K., Corresponding, Japanese Society of Microbial Ecology
Microbes and Environments, 2018, [Reviewed]

The dark septate endophytic fungus Phialocephala fortinii is a potential decomposer of soil organic compounds and a promoter of Asparagus officinalis growth
Surono; Kazuhiko Narisawa, There is limited information as to whether dark septate endophytic (DSE) fungi are able to degrade organic carbon, nitrogen and phosphorus compounds in soil and if these fungi have a significant role in nutrient cycles in nature, especially under organic nutrient conditions. In order to further knowledge in this area, 25 DSE fungi were isolated and tested for their promotion of Asparagus officinalis seedling growth. Three Phialocephala fortinii isolates were found to be most effective in increasing the growth of A. officinalis plants compared with uninoculated controls. These isolates had the ability to degrade all carbon and nitrogen compounds tested except for lignin. Using organic phosphorus and nitrogen sources, the three P. fortinii isolates were able to promote the growth of A. officinalis compared with control plants. The isolates were also able to promote the growth of A. officinalis seedlings on semi-organic and organic media. Our findings demonstrate that P. fortinii has a role in the promotion of A. officinalis growth under organic nutrient conditions, possibly by decomposing organic phosphorus and nitrogen compounds in soil. (C) 2017 Elsevier Ltd and British Mycological Society. All rights reserved., ELSEVIER SCI LTD
FUNGAL ECOLOGY, Aug. 2017, [Reviewed]

Soil survey on vegetation recovery of the abandoned cropland around Komado Shitsugen Moor, Fukushima Prefecture
SAKAGAMI Nobuo; INOSE Yuumi; GUO Yong; NISHIZAWA Tomoyasu; NARISAWA Kazuhiko; WATANABE Makiko, 【背景と目的】

本研究の対象地である福島県南会津町・昭和村に位置する駒止湿原は，1950年代に周辺地域が開拓地として開墾された。農地開発のためのブナ林伐採は1967年から1976年まで続き，抜根・整地のためにブナ林下の有機質表層が失われた。天然記念物に指定された1970年以降，湿原の保全対策が次第に強化され，農地からの土砂流入が湿原の生態系に影響を与えていることが指摘されると，2000年までに集水域全体が天然記念物として追加指定された。開拓農地跡は裸地化したが，2000年よりブナ林復元事業が実施された（図1）。2016年現在，ススキ草地の中に成長したブナ個体を認めることができるが，ブナ二次林辺縁部には，枯死あるいは成長していないブナの個体が多い一帯がある。

そこで，本研究では，植生の回復と有機質表層の再生との過程を明らかとするため，ブナ二次林から耕作放棄地であるススキ草地にかけて複数地点で土壌調査を行い，土壌断面の観察をおこなった。

【研究の方法】

駒止湿原周辺に分布するブナ二次林と開墾跡地を含むように，調査区（10×30m）を設置した。優占する植生の違いから，それぞれブナ林区，境界区，および放棄地区と区分し，コドラート内に自生する植生調査を行うとともに，コドラート両端および各区境界の4地点において土壌断面調査を実施した。また，各層位から土壌試料を採取し，一般理化学性の分析を行なった。

【結果と考察】

4地点における土壌断面調査の結果を図2に示す。農地開拓に伴う表土削剥の影響を受けていないブナ二次林下の2地点では，10cm以上A層が認められた（全炭素量20%以上）。そして，開拓跡地である放棄地区では，ススキの根が張る表層土壌もブナ二次林下層土に近い褐色を呈し，有機質表層の形成はほとんど認められなかった（全炭素量5%以下）。一方，ササが繁茂する境界区側の地点では，ササや他の下草の根が張る地表面から5cm程度の範囲において，比較的明度が低く全炭素量15%程度の有機質表層の分布が認められた。今後，ササおよびススキの繁茂と有機質表層形成との関係について調査し，ブナ林再生に向けた課題を明らかにするとともに，植生回復と土壌生成との関係に言及していく。, The Association of Japanese Geographers
Proceedings of the General Meeting of the Association of Japanese Geographers, 2017

Cladophialophora inabaensis sp nov., a New Species among the Dark Septate Endophytes from a Secondary Forest in Tottori, Japan
Erika Usui; Yusuke Takashima; Kazuhiko Narisawa, A novel species of Cladophialophora is herein described from the natural environment of secondary forest soil in Japan, which was able to be colonized by the host plant root. Morphological observations indicated that the isolate is distinct from previously identified species, and, thus, is described as the new species, C. inabaensis sp. nov., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Sep. 2016

Cladophialophora inabaensis sp nov., a New Species among the Dark Septate Endophytes from a Secondary Forest in Tottori, Japan
Erika Usui; Yusuke Takashima; Kazuhiko Narisawa, A novel species of Cladophialophora is herein described from the natural environment of secondary forest soil in Japan, which was able to be colonized by the host plant root. Morphological observations indicated that the isolate is distinct from previously identified species, and, thus, is described as the new species, C. inabaensis sp. nov., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Sep. 2016

Complete genome sequence of Streptomyces parvulus 2297, integrating site-specifically with actinophage R4
Nishizawa, T; T. Miura; C. Harada; Y. Guo; K. Narisawa; H. Ohta; H. Takahashi; M. Shirai, Am Soc Microbiol
Genome Announc., Aug. 2016, [Reviewed]

3-1-15 三宅島火山灰堆積物で形成された土壌層位における微生物群集構造解析（3-1 土壌生物の生態と機能，2016年度佐賀大会）
平野 明則; 郭 永; 西澤 智康; 上條 隆志; 成澤 才彦; 太田 寛行, 一般社団法人 日本土壌肥料学会
日本土壌肥料学会講演要旨集, 2016

Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongate.
Ohshima S; Sato Y; Fujimura R; Takashima Y; Hamada M; Nishizawa T; Narisawa K; Ohta H., An endohyphal bacterium (strain B1-EBT) living in association with the fungus Mortierella elongata FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EBT required cysteine for growth and grew at temperatures between 4 and 35 degrees C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EBT forms a distinct clade in the family Burkholderiaceae, encompassing a group of endosymbionts associated with several soil isolates of M. elongata. The most closely related genus is 'Candidatus Glomeribacter gigasporarum', an endosymbiont of the arbuscular mycorrhizal fungus Gigaspora margarita. The major cellular fatty acids of strain B1-EBT were C-16 : 0, summed feature 3 (C-16 : (1)omega 7c and C-16 : (1)omega 6c) and summed feature 8 (C-18 : (1)omega 7c or C-18 : (1)omega 6c). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EBT represents a novel genus and novel species in the family Burkholderiaceae, for which the name Mycoavidus cysteinexigens gen. nov., sp. nov. is proposed. The type strain is B1-EBT (=JCM 30646(T) =LMG 28693(T) =NBRC 110909(T))., SOC GENERAL MICROBIOLOGY
Int J Syst Evol Microbiol, 2016, [Reviewed]

岐阜県御嶽山の森林土壌から採取した菌核の培養法および非培養法による菌類群集解析　　　　　　　　　　　　　　　
野々山; 佐々木; 弥; 坂上伸生; 成澤才彦; 太田寛行; 渡邊眞紀子
土と微生物, 2016, [Reviewed]

三宅島2000年噴火堆積物中の窒素固定細菌に関する研究(2015年度大会講演要旨)
平野 明則; 郭 永; 藤村 玲子; 佐藤 嘉則; 西澤 智康; 上條 隆志; 成澤 才彦; 太田 寛行, 日本土壌微生物学会
土と微生物, 2015

土壌糸状菌Mortierella elongataに内生する細菌のゲノム特性の解析(2015年度大会講演要旨)
大島 翔子; 藤村 玲子; 水上 沙紀; 佐藤 嘉則; 西澤 智康; 成澤 才彦; 太田 寛行, 日本土壌微生物学会
土と微生物, 2015

Analysis of Sclerotia-Associated Fungal Communities in Cool-Temperate Forest Soils in North Japan
Anzilni F. Amasya; Kazuhiko Narisawa; Makiko Watanabe, We herein investigated sclerotia that were obtained from cool-temperate forests in Mt. Chokai and Mt. Iwaki in north Japan and tentatively identified as the resting bodies of Cenococcum geophilum. The profiles of sclerotia-associated fungal communities were obtained through T-RFLP combined with clone library techniques. Our results showed that sclerotia in Mt. Chokai and Mt. Iwaki were predominated by Arthrinium arundinis and Inonotus sp., respectively. The results of the present study suggested that these sclerotia-associated species were responsible for the formation of sclerotia or sclerotia were originally formed by Cenococcum geophilum, but were subsequently occupied by these species after Cenococcum geophilum germinated or failed to survive due to competition., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, 2015, [Reviewed]

The Role of Dark Septate Endophytic Fungal Isolates in the Accumulation of Cesium by Chinese Cabbage and Tomato Plants under Contaminated Environments
Ousmane Diene; Nobuo Sakagami; Kazuhiko Narisawa, Following the 2011 Fukushima Daiichi Nuclear Power Plant accident, the preservation of the food chain from radionuclides contamination has become of crucial importance. The potential of Dark septate endophytic fungi in the management of Cs accumulation in plants under contaminated environments was investigated using Chinese cabbage and tomato plants. Four endophytic fungal isolates of different species, i.e. Pseudosigmoidea ibarakiensis I.4-2-1, Veronaeopsis simplex Y34, Helminthosporium velutinum 41-1, and as yet unidentified taxon 312-6 were tested In Vitro in two levels of Cs (5ppm and 10ppm). On the plant growth, the inoculation of the selected DSEs to both Chinese cabbage and tomato resulted in an increased biomass of up to 82% and 122%, respectively compared to control (non-inoculated) plants. With regards to the Cs accumulation, it varied with the host plant considered. In Chinese cabbage, DSEs inoculation caused higher Cs accumulation in above ground plant parts, whereas in tomato, Cs accumulation decreased significantly with three of the isolates tested, i.e., V. simplex Y34, P. ibarakiensis I.4-2-1, and the as yet unidentified taxon 312-6 suggesting low-risk transfer on the above ground plants parts as a result of high and negative plant reactions rather than high and positive reactions as it is the case with Chinese cabbage. These results suggested that DSEs can be recommended for use with Chinese cabbage to enhance phytoremediation of Cs in surrounding contaminated areas. With tomato, DSEs can be recommended for decreasing the accumulation of Cs in plants under contaminated environments., PUBLIC LIBRARY SCIENCE
PLOS ONE, Oct. 2014, [Reviewed]

Effect of corn steep liquor on lettuce root rot (Fusarium oxysporum f.sp lactucae) in hydroponic cultures
Yufita D. Chinta; Kazuki Kano; Ani Widiastuti; Masaru Fukahori; Shizuka Kawasaki; Yumi Eguchi; Hideyuki Misu; Hiromitsu Odani; Songying Zhou; Kazuhiko Narisawa; Kazuki Fujiwara; Makoto Shinohara; Tatsuo Sato, BACKGROUND: Recent reports indicate that organic fertilisers have a suppressive effect on the pathogens of plants grown under hydroponic systems. Furthermore, microorganisms exhibiting antagonistic activity to diseases have been observed in organic hydroponic systems. This study evaluated the effect of corn steep liquor (CSL) on controlling lettuce root rot disease [Fusarium oxysporum f.sp. lactucae (FOL)] in a hydroponic system. The effect of CSL and Otsuka A (a chemical fertiliser) on the inhibition of FOL in terms of mycelial growth inhibition was tested in vivo.
RESULTS: Addition of CSL suppressed FOL infection rates. CSL inhibited FOL infection by 26.3-42.5% from 2 days after starting incubation. In comparison, Otsuka A inhibited FOL growth by 5.5-19.4%. In addition, four of 10 bacteria isolated from the nutrient media containing CSL exhibited inhibition zones preventing FOL mycelial growth.
CONCLUSIONS: We found that CSL suppressed FOL in lettuce via its antifungal and biostimulatory effects. We suggest that activation of beneficial microorganisms present in CSL may be used to decrease lettuce root rot disease and contribute to lettuce root growth. (C) 2014 Society of Chemical Industry, WILEY-BLACKWELL
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Aug. 2014, [Reviewed]

Heteroconium chaetospira Induces Resistance to Clubroot via Upregulation of Host Genes Involved in Jasmonic Acid, Ethylene, and Auxin Biosynthesis
Rachid Lahlali; Linda McGregor; Tao Song; Bruce D. Gossen; Kazuhiko Narisawa; Gary Peng, An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with the severity of clubroot at 5 weeks after treatment at a low (2x10(5) spores pot(-1)) but not high (2x10(5) spores pot(-1)) dose of pathogen inoculum. Transcript levels of nine B. napus (Bn) genes in roots treated with Hc plus Pb, Pb alone and a nontreated control were analyzed using qPCR supplemented with biochemical analysis for the activity of phenylalanine ammonia lyases (PAL). These genes encode enzymes involved in several biosynthetic pathways related potentially to plant defence. Hc plus Pb increased the activity of PAL but not that of the other two genes (BnCCR and BnOPCL) involved also in phenylpropanoid biosynthesis, relative to Pb inoculation alone. In contrast, expression of several genes involved in the jasmonic acid (BnOPR2), ethylene (BnACO), auxin (BnAAO1), and PR-2 protein (BnPR-2) biosynthesis were upregulated by 63, 48, 3, and 3 fold, respectively, by Hc plus Pb over Pb alone. This indicates that these genes may be involved in inducing resistance in canola by Hc against clubroot. The upregulation of BnAAO1 appears to be related to both pathogenesis of clubroot and induced defence mechanisms in canola roots. This is the first report on regulation of specific host genes involved in induced plant resistance by a non-mycorrhizal endophyte., PUBLIC LIBRARY SCIENCE
PLOS ONE, Apr. 2014, [Reviewed]

Characterization of Early Microbial Communities on Volcanic Deposits along a Vegetation Gradient on the Island of Miyake, Japan
Yong Guo; Reiko Fujimura; Yoshinori Sato; Wataru Suda; Seok-won Kim; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Kazuhiko Narisawa; Hiroyuki Ohta, The 2000 eruption of Mount Oyama on the island of Miyake (Miyake-jima) created a unique opportunity to study the early ecosystem development on newly exposed terrestrial substrates. In this study, bacterial and fungal communities on 9- and 11-year-old volcanic deposits at poorly to fully vegetation-recovered sites in Miyake-jima, Japan, were characterized by conventional culture-based methods and pyrosequencing of 16S rRNA and 18S rRNA genes. Despite the differences in the vegetation cover, the upper volcanic deposit layer samples displayed low among-site variation for chemical properties (pH, total organic carbon, and total nitrogen) and microbial population densities (total direct count and culturable count). Statistical analyses of pyrosequencing data revealed that the microbial communities of volcanic deposit samples were phylogenetically diverse, in spite of very low-carbon environmental conditions, and their diversity was comparable to that in the lower soil layer (buried soil) samples. Comparing with the microbial communities in buried soil, the volcanic deposit communities were characterized by the presence of Betaproteobacteria and Gammaproteobacteria as the main bacterial class, Deinococcus-Thermus as the minor bacterial phyla, and Ascomycota as the major fungal phyla. Multivariate analysis revealed that several bacterial families and fungal classes correlated positively or negatively with plant species., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Apr. 2014, [Reviewed]

Control of Fusarium wilt in melon by the fungal endophyte, Cadophora sp.
Khastini; R. O.; Sato; Y.; Ogawara; T.; Narisawa; K., Two soil-borne fungal endophytes almost completely suppressed the effects of a post-inoculated and virulent strain of Fusarium oxysporum f. sp. melonis when inoculated to axenically reared melon seedlings in Petri dishes. They were identified as Cadophora sp. on the basis of ITS 1-5.8S rDNA-ITS 2 sequences and morphological characters and obtained from the roots of Chinese cabbage grown as bait plants in a mixed soil made up of samples from different forest soils from Alberta and British Columbia, Canada. Hyphae of Cadophora sp. grew along the surface of the root and colonized root cells of the cortex and reduced the ingress of the Fusarium pathogen into adjacent cells. Melon seedlings pre-inoculated with Cadophora sp. were also grown in soil amended with the different N sources, nitrate or the amino acids leucine and valine, and glucose (final C:N ratio = 10:1). After 4 weeks, these seedlings were transplanted into the field and disease symptoms were assessed. Only the endophyte-inoculated seedlings treated with valine could effectively inhibit the development of Fusarium wilt in two plots and reduced disease symptom development by 43 and 62 %., SPRINGER
Eur J Plant Pathol, 2014, [Reviewed]

Draft genome sequence of the betaproteobacterial endosymbiont associated with the fungus Mortierella elongata FMR23-6
Reiko Fujimura; Ayumu Nishimura; Shoko Ohshima; Yoshinori Sato; Tomoyasu Nishizawa; Kenshiro Oshima; Masahira Hattori; Kazuhiko Narisawa; Hiroyuki Ohta, The fungus Mortierella elongata FMR23-6 harbors an endobacterium inside its mycelium. Attempts to isolate the endobacterium from the fungus were not yet successful, but a highly purified bacterial fraction was prepared. Here, we report the draft genome sequence of the endobacterium., American Society for Microbiology
Genome Announcements, 2014, [Reviewed]

First Report on Fungal Symbionts of Lycopodiaceae Root from Mount Gede Pangrango National Park Indonesia.
Takashima Y; Narisawa K; Hidayat I; and Rahayu G., Association of arbuscular mycorrhizal fungi (AMF) with plant roots is often stated as critical to the successful establishment and diversification of terrestial plants. Information on AMF associations with Lycopodiaceae, ancient lineages of terrestrial plants, contributes to the understanding of terrestrial colonization of plants. While diversity of AMF and other fungal endophytes of some agricultural plants in Indonesia is extensively studied, terresterial Lycopodiaceae are not well documented. In this study, colonization status of AMF and root endophytic fungi (REF) in sporophytes of terresterial Lycopodiaceae (Huperzia selago, H. serrata and Lycopodium clavatum) inhabiting Mt. Pangrango at different altitudes were investigated on the basis of root microscopic observations and culture-dependent methods, respectively. As results, AMF colonization was observed in H. selago collected from near summit (NS, 2998 m asl) and H. serrata collected from Kandang Badak (KB, 2440 m asl) and Cibereum Water Fall (CF, 1728 m asl), but not in L. clavatum from Mount Pangrango Summit (PS, 3019 m asl). These results indicate that the colonization rate of AMF is affected by differences in altitude. REF isolates consisting of seven genera including Cadophora, Cladophialophora, Cryptosporiopsis, Leohumicola, Leptodontidium, Phialocephala, Pseudoclathrosphaerina (putatively similar to Meliniomyces), and ecologically uncertain Helotiales were obtained. These isolates contained putative dark septate endophytes (DSE) and ericoid mycorrhiza and were more diverse around sub-alpine areas (NS, KB and PS sites) than in motane areas (CF site). The presence of these REF may be affected by a complex and mixed plant vegetation in the transition zone between the montane and sub-alpine areas., Agricultural and Forestry Research Center, University of Tsukuba
Journal of Developments in Sustainable Agriculture, 2014

Heat shock-induced resistance in strawberry against crown rot fungus Colletotrichum gloeosporioides
Ani Widiastuti; Mioko Yoshino; Harufumi Saito; Keisuke Maejima; Songying Zhou; Hiromitsu Odani; Kazuhiko Narisawa; Morifumi Hasegawa; Youji Nitta; Tatsuo Sato
Physiological and Molecular Plant Pathology, Oct. 2013, [Reviewed]

糸状菌Mortierella elongataの菌糸内部に生息する細菌の全ゲノム解析(最優秀賞,JSME2012豊橋大会特集)
藤村 玲子; 西村 歩; 佐藤 嘉則; 大島 翔子; 大島 健志朗; 服部 正平; 成澤 才彦; 太田 寛行, 日本微生物生態学会
日本微生物生態学会誌, 2013, [Reviewed]

Pseudosigmoidea ibarakiensis sp. nov., a Dark Septate Endophytic Fungus from a Cedar Forest in Ibaraki, Japan.
Diene; O.; Wang; W. T.; Narisawa; K., A dark septate fungus of Pseudosigmoidea, Hyphomycetes, was recovered from forest soil in Ibaraki prefecture, Japan. The isolate is characterized by pale to brown conidia with up to 8 septa measuring 68-132 × 4-7.9 μm. It is also unique in producing conidia borne by long conidogenious cells in agar medium with or without water, compared to P. cranei, which must be immersed in water to sporulate. Morphological analysis indicated that the isolate is distinct from P. cranei and is described as a new species, P. ibarakiensis sp. nov. Pathogenicity tests of Chinese cabbage and cucumber seedlings indicated that the fungus grows as an endophyte and colonizes, inter and intracellularly, the root epidermal and cortical layers without causing apparent disease symptoms in the host. This endophyte showed the ability to support cucumber plant growth under conditions where NaNO3 was replaced by organic nitrogen but also conferred to Chinese cabbage the ability to grow at low pH. It also became successfully established in six other plants, including the Brassicae, Solanaceae, Poaceae, and Liliacea families, suggesting its adaptability to a broad range of host plants.
Microbes and Environments, 2013, [Reviewed]

A new fungal endophyte, Scolecobasidium humicola, promoting growth of tomato under organic nitrogen conditions
Rola S. Mahmoud and Narisawa; K, A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources., PUBLIC LIBRARY SCIENCE
PLoS ONE, 2013, [Reviewed]

A Trial astudy of Nature Observation Program Using Sclerotia from Forest Soils
N. Sakagami; A, Sato; Y. Kodaka; M.Watanabe; K. Narisawa
Environmental Education, Oct. 2012, [Reviewed]

The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage
Rida O. Khastini; Hiroyuki Ohta; Kazuhiko Narisawa, The soil-inhabiting fungal pathogen Fusarium oxysporum has been an increasing threat to Chinese cabbage (Brassica campestris L.). A dark septate endophytic fungus, Veronaeopsis simplex Y34, isolated from Yaku Island, Japan, was evaluated in vitro for the ability to suppress Fusarium disease. Seedlings grown in the presence of the endophyte showed a 71% reduction in Fusarium wilt disease and still had good growth. The disease control was achieved through a synergetic effect involving a mechanical resistance created by a dense network of V. simplex Y34 hyphae, which colonized the host root, and siderophore production acting indirectly to induce a resistance mechanism in the plant. Changes in the relative abundance of the fungal communities in the soil as determined by fluorescently labelled T-RFs (terminal restriction fragments), appeared 3 weeks after application of the fungus. Results showed the dominance of V. simplex Y34, which became established in the rhizosphere and out-competed F. oxysporum., MICROBIOLOGICAL SOCIETY KOREA
THE JOURNAL OF MICROBIOLOGY, 2012, [Reviewed]

A New Fungal Endophyte, Helminthosporium velutinum, Promoting Growth of a Bioalcohol Plant, Sweet Sorghum.
Ousmane Diene; Takuya Takahashi; Akane Yonekura; Youji Nitta; and Kazuhiko Narisawa, A new fungal isolate that grows endophytically in sweet sorghum was identified as Helminthosporium velutinum Link ex Ficinus & Schubert. Light-microscopy of cross-sections of colonized sweet sorghum roots showed that the intercellular, pigmented hyphae of the fungus was mostly limited to the epidermal layer and formed outer mantle-like structures. This endophyte has the ability to significantly increase sweet sorghum biomass. This is the first report of Helminthosporium as an endophyte and could help realize sustainable the biomass production for biofuel purposes., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
Microbes and Environments, 2010, [Reviewed]

Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata Soil Isolates.
Sato; Y.; Narisawa; K. Tsuruta; K.; Umezu; M.; Nishizawa; T. Tanaka; K.; Yamaguchi; K.; Komatsuzaki; M.; H. Ohta, Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N(2)O-producing activity and the presence of endobacteria., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
Microbes and Environments, 2010, [Reviewed]

森林土壌から検出される菌核の微生物担体としての機能　　　　　　　　　　　　　　　
野々山 弥; 成澤才彦; 太田寛行; 渡邊眞紀子
日本生物工学会誌, 25 Nov. 2009, [Reviewed]

森林土壌に形成される菌核の微生物担体としての有効性評価
野々山弥; 太田寛行; 成澤才彦; 渡邉眞紀子, Fungal sclerotia, the resting bodies formed by ectomycorrhizal fungi, are found in forest soils and characterized by their hard, black, and spherical structure. Bacteria are detected in their porous internal structure of the sclerotium. It has been known that porous materials containing microbial cells have an advantage to maintain activities of useful microbes in soil. The objective of this study is to characterize phylogenetically the bacterial community inside the sclerotium grains and evaluate the efficiency of sclerotia as microbial cell carrier. Bacteria were isolated from four sclerotia by culturing on DNB agar medium and isolates were subjected to 16S rRNA gene analysis. The autoclave-treated sclerotium was then immersed in a pure culture of the isolated bacteria to check the behavior of bacteria. The experiment using eight strains including Ralstnia pickettii, demonstrated that the maximum counts of viable cell found in sclerotium and rate of cell adsorption into sclerotium were 8.7×10^7CFU・g^<-1> and 10^2-10^6CFU・h^<-1>, respectively., 日本生物工学会
生物工学会誌, 24 Nov. 2009, [Reviewed]

The use of symbiotic fungal associations with crops in sustainable agriculture
Diene; O. and Narisawa; K, Agriculture in the 21st century faces the daunting task of satisfying the unceasingly increasing demand for food in a context of continuous depletion of natural resources and the need to respect international environmental standards. Among the alternatives to conventional agriculture developed in this context, symbiotic fungal association with crops shows considerable promise because of its effectiveness, habit-specific mode of action, and ability to provide multiple benefits. Known as endophytism, this association represents a new area of research based on the benefits of mutualistic interactions between host crops and nonpathogenic fungi. The advantages conferred by endophytic fungi include their ability to promote plant growth and tolerance of both abiotic stresses (e.g., salt, drought, heat) and biotic stresses (e.g., insects, plant diseases). As such, the practical applications of endophytes as potential sources of bioorganic nutrients and as biocontrol agents can significantly improve yields in an environmentally sound way. Moreover, the ability of fungal endophytes to improve plant tolerance of salt, drought, and heat stress make it possible to grow crops on previously uncultivable land. Thus, fungal endophytes should be included among alternative modern technologies to support food production., Agricultural and Forestry Research Center, University of Tsukuba
Journal of Developments in Sustainable Agriculture, 2009

Molecular characterization and endophytic nature of the root-associated fungus Meliniomyces variabilis (LtVB3)
Nobuaki Ohtaka; Kazuhiko Narisawa, The root-associated fungus LtVB3 was formerly reported as a potential, new biocontrol agent of Verticillium yellows of Chinese cabbage and Fusarium wilt of tomato. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions and morphological characteristics, LtVB3 is here identified as Meliniomyces variabilis, a recently described species [formerly called "Variable White Taxon'' (VWT)] in a new genus erected to accommodate sterile fungi with phylogenetic affinities to Rhizoscyphus ericae, an ericoid mycorrhizal fungus. In vitro inoculation experiments showed that LtVB3 colonized the roots of torch azalea and eucalyptus; however, growth was enhanced only in azalea but not in eucalyptus. Hyphae of M. variabilis formed intracellular structures typical of ericoid mycorrhizas in epidermal cells of azalea roots but did not produce any typical ectomycorrhizal structures with or within root cells of either host. In LtVB3-treated eucalyptus roots, some cells in the epidermal and cortical layers had wall appositions and thickenings, which appeared to restrict fungal growth., SPRINGER TOKYO
JOURNAL OF GENERAL PLANT PATHOLOGY, Feb. 2008, [Reviewed]

Molecular characterization and ecological nature of the dark septate endophytic fungus LtVB3, a biocontrol agent of Verticillium yellows in Chinese cabbage　　　　　　　　　　　　　　　
Ohtaka; N. and Narisawa; K.
Journal of General Plant Pathology, 2008, [Reviewed]

Suppression of rice blast with application of freeze-killed mycelia of the new biocontrol fungal candidate MKP5111B
Ohtaka; N.; Kawamata; H. and Narisawa; K., Several applications of the suppressive fungus MKP5111B, isolated from the phylloplane of rice plants, were tested in an effort to control rice blast disease. Three treatments with MKP5111B [ living (Std), killed with liquid nitrogen (FR), and autoclaved (AC)] were either sprayed onto rice seedlings or mixed into seed-sown soil. Three weeks after spraying and 4 weeks after the soil application, we introduced Magnaporthe oryzae, the causal agent of rice blast, into our systems. The Std and FR treatments suppressed rice blast, but the AC treatment proved ineffective. Although a suppressive effect was seen on new leaves, no mycelium of MKP5111B was seen. The fungus thus may have induced a systemic resistance in the rice plants. A substance from MKP5111B, such as elicitor molecule(s) are likely responsible for the induced resistance., SPRINGER TOKYO
Journal of General Plant Pathology, 2008, [Reviewed]

A mutualistic symbiosis between a dark, septate endophytic fungus, Heteroconium chaetospira, and a non-mycorrhizal plant, Chinese cabbage, with bi-directional nutrient transfer
Usuki; F. and Narisawa; K, Symbiotic microorganisms, such as mycorrhizal fungi, are known to associate with most plants; however members of the Cruciferae are an exception. We investigated nutrient exchange between a dark septate endophytic fungus, Heteroconium chaelospira, and Chinese cabbage plants (Cruciferae) in vitro. Chinese cabbage could not use some amino acids, while the fungus-treated plants were able to use all of the nitrogen forms provided. To demonstrate that nitrogen transfer occurs between the fungus and the host plant, we used a hydrophobic polytetrafluoroethylene (PTFE) membrane compartment system, which restricts diffusion and mass flow of ions and allows only fungal penetration. Our results strongly suggest that H. chaetospira provided nitrogen to the plant, rather than the plant mineralizing available organic nitrogen. In addition carbon transfer from the host plant to the fungus was demonstrated with HPLC and (CO2)-C-13-labeling experiments. When H. chaetospira colonized host plant roots under low glucose condition, ergosterol content in culture pot (as an index of fungal biomass) increased significantly compared to the fungal treatment without a host plant. Sucrose concentration in the host root significantly decreased as a result of fungal colonization, and mannitol (a specific carbon source to fungal cells) increased in the roots. Sucrose and mannitol in the host root treated with the fungus were labeled clearly by C-13 after C-13-labeled CO2 was provided to the plant. These results suggest that the fungus obtained carbon, mainly as sucrose, from the host plant. We show for the first time the existence of a fungus establishing a mutualistic association with a nontnycorrhizal Cruciferae plant., ALLEN PRESS INC
Mycologia, 2007, [Reviewed]

Heteroconium chaetospira, a dark septate root endophyte allied to the Herpotrichiellaceae (Chaetothyriales) obtained from some forest soil samples using bait plants
Narisawa; K.; Hambleton; S. and Currah; R. S., During an extended search in Western Canada for fungal root endophytes useful as biocontrol agents against soil-borne pathogens, we isolated Heteroconium chaetospira, as well as Phialocephala fortinii or similar taxa, from seven samples of forest soil using herbaceous seedlings of four different species (i.e., barley, Chinese cabbage, eggplant, and melon) as bait plants. Our results support a previous observation that eggplant is a particularly effective species for baiting H. chaetospira from soil and confirm the ability of this fungus to grow as an endophyte in the roots of axenically reared host plants. Cultural characters show that this species is similar to P. fortinii and other melanized fungi in the dark septate endophyte (DSE) group (e.g., Leptodontidium orchidicola, P. sphaeroides, and Cadophora finlandica) in that it produces darkly pigmented colonies on agar media. Heteroconium chaetospira differs from P. fortinii and other melanized members of the Leotiomycetes in the DSE group in that its conidia are fusiform and develop in blastic acropetal chains. Heteroconium chaetospira is phylogenetically distant from most DSE taxa because DNA sequences for the nuclear small subunit (SSU) ribosomal RNA gene (rDNA) indicate that the taxon is affiliated with the Herpotrichiellaceae of the Chaetothyriales rather than with the Leotiomycetes. © 2007 The Mycological Society of Japan and Springer-Verlag., Springer Japan
Mycoscience, 2007, [Reviewed]

Effects of pathogen density, soil moisture, and soil pH on the biological control of clubroot in Chinese cabbage by the root endophytic fungus Heteroconium chaetospira.
Narisawa; K.; Shimura; M.; Usuki; F.; Fukuhara; S. and Hashiba; T., The effects of soil moisture and pH, and pathogen resting spore density, on the effectiveness of the biological control of clubroot by the fungal endophyte Heteroconium chaetospira was evaluated in greenhouse and field experiments. Conditions favoring disease development included low pH (5.5) and high soil moisture content (80%), with significant reductions in the disease being observed at a higher pH (6.3 and 7.2) and lower soil moisture content (40 and 60%). In greenhouse tests, H. chaetospira effectively controlled clubroot (reducing the disease by 90 to 100%) at pathogen resting spore densities of 104 and 105 spores/g of soil at all soil pHs tested (5.5, 6.3, and 7.2). However, when the resting spore density was 106 spores/g of soil, plants were severely diseased, regardless of treatment, and H. chaetospira had no effect on disease. At a soil moisture content of 40%, disease occurrence was low, regardless of pathogen spore density, but disease was significantly lower in H. chaetospira -treated plants at pathogen spore density of 105 spores/g of soil. At 60% soil moisture content, H. chaetospira significantly could affect at pathogen spore densities of 10(4) and 10(5) but not 10(4)/g of soil. At 80% soil moisture content, there was no effect of H. chaetospira at pathogen density. In situ, the soil moisture contents were constantly adjusted to relatively low to moderate (pF 2.2 to 2.4 and pF 2.0 to 2.2) and high (pF 1.6 to 1.8). Other environmental conditions, such as resting spore density and soil pH, were maintained at constant levels. Control plants (not treated with H. chaetospira) showed uniformly high disease levels and proportions of diseased plants across all three moisture treatments (disease index = 72 to 80, proportion of diseased plants 85 to 97%). In the field, H. chaetospiratreated plants at low soil moisture (pF 2.2 to 2.4, plot 1) had 68% disease reduction compared with untreated controls and 49% reduction at moderate moisture pF (pF 2.0 to 2.2, plot 2). There was no effect on disease by H. chaetospira at high soil moisture (pF 1.6 to 1.8, plot 3). Based on our results. H. chaetospira is an effective biocontrol agent against clubroot in Chinese cabbage at a low to moderate soil moisture range and a pathogen resting spore density of 105 (or lower) resting spores per gram of soil in situ., AMER PHYTOPATHOLOGICAL SOC
Plant Disease, 2005, [Reviewed]

Formation of ericoid mycorrhizal structures by the root endophytic fungus Heteroconium chaetospira with Rhododendron obtusum var. kaempferi
Fumiaki Usuki; Kazuhiko Narisawa, A resynthesis study was conducted to clarify the relationship between the root endophyte, Heteroconium chaetospira and the ericaceous plant, Rhododendron obtusum var. kaempferi. The host plant roots were recovered 2 months after inoculation, and the infection process and colonization pattern of the fungus were observed under a microscope. The hyphae of H. chaetospira developed structures resembling ericoid mycorrhizas, such as hyphal coils within the host epidermal cells. These structures were morphologically the same as previously reported ericoid mycorrhizal structures. The frequencies of hyphal coils within the epidermal cells of host roots ranged from 13 to 20%. H. chaetospira did not promote or reduce host plant growth. This is the first reported study that H. chaetospira is able to form structures resembling mycorrhizas within the roots of ericaceous plants., SPRINGER
Mycorrhiza, 2005, [Reviewed]

The development and endophytic nature of the fungus Heteroconium chaetospira
Teruyoshi Hashiba; Kazuhiko Narisawa, The root endophytic fungus Heteroconium chaetospira was isolated from roots of Chinese cabbage grown in field soil in Japan. This fungus penetrates through the outer epidermal cells of its host, passes into the inner cortex, and grows throughout the cortical cells, including those of the root tip region, without causing apparent pathogenic symptoms. There are no ultrastructural signs of host resistance responses. H. chaetospira has been recovered from 19 plant species in which there was no disruption of host growth. H. chaetospira has a symbiotic association with Chinese cabbage. The fungus provides nitrogen in exchange for carbon. These associations are beneficial for the inoculated plants, as demonstrated by increased growth rate. When used as a preinoculum, H. chaetospira suppresses the incidence of clubroot and Verticillium yellows when the test plant is post-inoculated with the causal agents of these diseases. H. chaetospira is an effective biocontrol agent against clubroot in Chinese cabbage at a low to moderate soil moisture range and a pathogen resting spore density of 105 resting spores per gram of soil in situ. Disease caused by Pseudo totuts syringae pv. macricola and Alternaria brassicae on leaves can be suppressed by treatment with H. chaetospira. The fungus persists in the roots and induces systemic resistance to the foliar disease. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
FEMS Microbiol. Letters, 2005

Anatomical study on the interaction between the root endophytic fungus Heteroconium chaetospira and Chinese cabbage
Miho Yonezawa; Fumiaki Usuki; Kazuhiko Narisawa; Jun Takahashi; Teruyoshi Hashiba, Chinese cabbage roots colonized by the dematiaceous fungal taxon Heteroconium chaetospira were previously found to become highly resistant to clubroot and Verticillium yellows. The dematiaceous fungus possesses an endophytic nature, but no detailed anatomical studies on endophyte-host plant interactions have so far been provided. Light and electron microscopy revealed that hyphae of H. chaetospira were abundant on and inside the root epidermal cells by 3 weeks following inoculation. The penetration pegs easily breached into epidermal cells, and the infection hyphae penetrated into cortical cells. Some appressorium-like swollen structures formed from intracellular hyphae, but no visible degradation of the host cell walls was evident where the hyphae contacted. No visible signs of host reactions and no invagination of the host plasma membrane around the hyphae were seen in the host cells. By 8 weeks following inoculation, masses of closely packed fungal cells had been formed in some cells of the epidermis and cortical layers, but further hyphal ingress was halted, mostly in the inner cortical cell layer. Thus, root vascular cylinders remained intact. © The Mycological Society of Japan and Springer-Verlag 2004., Springer Japan
Mycoscience, 2004, [Reviewed]

Suppression of rice blast by phylloplane fungi isolated from rice plant
Hitoshi Kawamata; Kazuhiko Narisawa; Teruyoshi Hashiba, Rice phylloplane fungi were evaluated for their potential as biocontrol agents for rice blast disease caused by Magnaporthe grisea. A total of 1923 fungal isolates were obtained from rice plants in fields at Ishigaki and Iwama and from potted plants placed in a cedar woods in Iwama as bait. Although 82.9% of isolated fungi could not be identified, species of Epicoccum were the most prevalent among identified isolates. Of the 1923 isolates, 967 were randomly selected for screening against rice leaf blast. Nine isolates (MKP5111B, MKP5112, J2JMR3-2, K2J131-2, I5R3-1, NOP541, K1KM134-1, NOP5112, MKP33222) suppressed the disease when a conidial or hyphal suspension of both the phylloplane fungus and pathogen were simultaneously used to inoculate rice plants cultured in pots in a growth chamber. Five of the isolates originated from potted plants in the woods and four from Ishigaki, a subtropical island. Five (MKP5111B, MKP5112, NOP541, NOP5112, MKP33222) of the nine isolates strongly suppressed conidial germination of M. grisea (≤0.7%) and formed inhibition zones (3-5 mm width) in dual cultures with the pathogen. Methanol extracts from the isolates also inhibited mycelial growth of the pathogen. These results suggest that the five isolates produced antibiotic(s). These five isolates are likely identical or closely related fungal species because the sequence of their ITS regions were 100% similar. ITS sequence analysis also suggested that J2JMR3-2 was associated with a species of Fusarium. Under field conditions, J2JMR3-2 reduced both leaf and panicle blast severity, and three other isolates (MKP5111B, K1KM134-1, K2J131-2) suppressed leaf blast in one of the three experiments.
J. Gen. Plant Pathol., 2004, [Reviewed]

Control of Verticillium yellows in Chinese cabbage by the Dark-septate endophytic fungus LtVB3
Kazuhiko Narisawa; Fumiaki Usuki; Teruyoshi Hashiba, Three hundred forty-nine fungal endophytes were obtained from a total of 1,214 root segments of eggplant, melon, barley, and Chinese cabbage grown as bait plants in a mixed soil made Lip of samples from different forest soils in Alberta and British Columbia. Canada. Three of the 349 isolates. when inoculated in axenically reared Chinese cabbage seedlings brown in petri dishes. almost completely Suppressed the effects of a post-inoculated and virulent strain of Verticillium longisporum. Two isolates effective against the pathogen were Phialocephala fortinii, which had been obtained from the roots of eggplant and Chinese cabbage. The third isolate was a dark septate endophytic (DSE) fungus obtained from barley roots. Hyphae of P fortinii grew along the Surface of the root and formed microsclerotia on or in the epidermal layer. Hyphae of the DSE fungus heavily colonized root cells of the cortex. Seedlings grown for 1 week in the presence of the endophytes were then challenged with the Verticillium pathogen. In DSE-treated roots. some of cell walls in the epidermal and cortical layers showed cell wall appositions and thickenings, which appeared to limit the ingress of the pathogen into adjacent cells. Such marked host reactions were not observed in the root cells colonized by P. fortinii. Chinese cabbage preinoculated with the above endophytes and. for comparison, a previously reported disease-suppressive fungal endophyte, Heteroconium chaetospira, were transplanted into the field and disease symptoms were assessed. The DSE could most effectively inhibit the development of Verticillium yellows. with reductions in the percentages of external and internal disease symptoms of 84 and 88%. respectively. The protective values against the disease are extremely high compared with those of other isolates. Most of the DSE-treated plants, in the plots achieved marketable quality., AMER PHYTOPATHOLOGICAL SOC
Phytopathology, 2004, [Reviewed]

Mutualistic symbiosis between Heteroconium chaetospira and Chinese cabbage　　　　　　　　　　　　　　　
Narisawa; K.
CBA/ABC & MAPB Annual Meeting, Winnipeg, Canada, 2004, [Reviewed], [Invited]

Ultrastructural Study on Interaction between a Sterile, White Endophytic Fungus and Eggplant Roots.
Narisawa; K.; Chen; M.; Hashiba; T. and Tsuneda; A
Journal of General Plant Pathology, 2003, [Reviewed]

Induced systemic resistance of Chinese cabbage to bacteria leaf spot and Alternaria leaf spot by root endophytic fungus, Heteroconium chaetospira　　　　　　　　　　　　　　　
Morita; S.; Azuma; M.; Aoba; T.; Satou; H.; Narisawa; K. and Hashiba; T.
Journal of General Plant Pathology, 2003, [Reviewed]

Suppression of Verticillium yellows in Chinese cabbage by the root endophytic fungus, Phialocephala fortinii　　　　　　　　　　　　　　　
Narisawa, K; Currah, R. S; Hashiba
8th ICPP, 2003, [Reviewed]

An efficient inoculation method for colonization of Chinese cabbage seedlings by the root endophytic fungus Heteroconium chaetospira
Usuki; F.; Narisawa; K.; Yonezawa; M. and Hashiba; T., To develop an efficient method to inoculate Chinese cabbage seedlings with the root endophytic fungus Heteroconium chaetospira, an appropriate nursery soil and glucose concentration for the nutrient medium for fungal colonization were determined. A grid-sheet method was established for estimating the degree of colonization of entire roots by the fungus. The fungus colonized at high frequencies when peat moss was used as the rooting medium. Colonization was highest (75%) when peat moss was amended with 0.1% glucose. Under these conditions, fungal hyphae developed intracellularly in root cortical cells. In contrast, under high glucose conditions, fungal colonization was restricted mostly to intercellular regions of epidermal or cortical root tissues. Here, hyphae formed inter- or intracellular microsclerotia. (Received August 19, 2002 ; Accepted December 5, 2002), Phytopathological Society of Japan
Journal of General Plant Pathology, 2002, [Reviewed]

Suppression of Verticillium wilt in Eggplant by some fungal root endophytes
Narisawa; K.; Kawamata; H.; Currah; R. S. and Hashiba; T., One hundred and twenty-three fungal isolates were obtained from 225 root segments of eggplants, melon, tomato, strawberry and Chinese cabbage, grown as bait plants in a mixed soil made up of samples from different fields in Shizuoka, Japan. Isolates belonging to Mycelium radicis atrovirens (MRA), including Phialocephala fortinii, were the most prevalent in all the five bait plants. Eleven of the 123 isolates, after being inoculated onto axenically reared eggplant seedlings, almost completely suppressed the pathogenic effects of a post-inoculated, virulent strain of Verticillium dahliae. Seven of these 11 isolates had come from the roots of eggplant and included Heteroconium chaetospira, P. fortinii, and unidentified species of Fusarium, Penicillium, Trichoderma and MRA. P. fortinii, H. chaetospira, a non-sporulating isolate with white mycelium (SWM) and MRA were easily reisolated from root segments. Hyphae of H. chaetospira, P. fortinii and SWM colonized the root tissues of eggplant without causing apparent pathogenic symptoms. The mechanisms by which these endophytes confer resistance to infection by V. dahliae are unknown but the effectiveness of these fungi in a laboratory setting indicates that they have potential as biocontrol agents and merit further investigation., KLUWER ACADEMIC PUBL
Eur. J. Plant Pathol, 2002, [Reviewed]

Colonization process of the root endophytic fungus Heteroconium chaetospira in root of Chinese cabbage
Ohki; T.; Masuya; H.; Yonezawa; M.; Usuki; F.; Narisawa; K. and Hashiba; T., Dark septate endophytic fungi (DSE) may have an important functional relationship with host plants, but these functions and the colonization process remain unknown. We made microscopic observations of the growth of an endophytic hyphomycete in Chinese cabbage roots to understand its colonization process. This hyphomycete was Heteroconium chaetospira, a suspected DSE. Three weeks post inoculation, some hyphae became irregularly lobed and formed microsclerotia within host epidermal cells of healthy plants. In stunted plants, hyphae formed closely packed masses of fungal cells within host epidermal cells, but conidiophores rarely broke through the cell walls to produce conidia., Springer Japan
Mycoscience, 2002, [Reviewed]

Ultrastructual effects of a fungal endophyte in the roots of eggplant and their potential role in disease suppression　　　　　　　　　　　　　　　
Narisawa; K.; Currah; R. S.; Chen; M.; and Tsuneda
ICOM3, 2001, [Reviewed]

Selection of root endophytic fungi to suppress Verticillium wilt in eggplant　　　　　　　　　　　　　　　
Narisawa; K.; Kawamata; H.; Currah; R. S.; and Hashiba; T.
CPS-APS Joint meeting, 2001, [Reviewed]

Impact of resistant melon cultivars on Sphaerotheca fuliginea
Hosoya; K.; Kuzuya; M.; Murakami; T.; Kato; K.; Narisawa; K. and Ezura; H., Effects of the resistant cultivars of melon on the powdery mildew fungus, Sphaerotheca fuliginea, were investigated in a melon-growing area of Japan. Powdery mildew fungi were isolated from four melon cultivars ('HN21','Quincy', 'Earl's Knight Natsu 2' and 'Earl's Miyabi Natsu 2') which are resistant to different races of S. fuliginea. 'HN21' is resistant to races 1 and N1, 'Quincy' and 'Earl's Knight Natsu 2' are resistant to races 1 and N2, and 'Earl's Miyabi Natsu 2' is resistant to races 1, N1 and N2. All 155 strains isolated from 'HN21' were identified as race N2. Seventy out of 81 strains isolated from 'Quincy' and 'Earl's Knight Natsu 2' were identified as race N1, while other strains were identified as races 1, 2US, N2 and N3. Nineteen out of 29 strains isolated from 'Earl's Miyabi Natsu 2' were identified as race N3, while other strains were identified as races 1, 2US, N1 and N4. These results suggest that, although the resistant cultivars are extremely effective for protecting the plants from their respective races, a race succession rapidly takes place, even in a small area, and other minor races become predominant., BLACKWELL WISSENSCHAFTS-VERLAG GMBH
Plant Breeding, 2000, [Reviewed]

Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by the root endophytic fungus, Heteroconium chaetospira
Narisawa; K.; Ohki; T. and Hashiba; T., Chinese cabbage seedlings inoculated with an isolate of the hyphomycete, Heteroconium chaetospira, were transplanted to the field. After 3 months, they showed a 52-97% reduction in clubroot and a 49-67% reduction in Verticillium yellows compared with noninoculated controls. H. chaetospira colonized the cortical cells, especially in the root tip region. Infected plants showed no disease symptoms. The infection process involves the formation of appressoria on the cell surface and the subsequent growth of hyphae within cells. H. chaetospira colonized 18 plant species, indicating a wide range of hosts. It may have potential as a biocontrol agent for clubroot and Verticillium yellows., BLACKWELL SCIENCE LTD
Plant Pathology, 2000, [Reviewed]

Race identification in powdery mildew(Sphaerotheca fuliginea)of melon(Cucumis melo L.)in Japan.　　　　　　　　　　　　　　　
Hosoya, K; Narisawa, K; Pitrat, M; Ezura, H
Plant Breeding, Jan. 1999, [Reviewed]

Powdery Mildew Fungi of Melon (Cucumis melo L.) identified by Differential Genotypes in Japan　　　　　　　　　　　　　　　
Hosoya; K.; Narisawa; K.; Pitrat; M.; and Ezura; H.
Plant Breeding, 1999, [Reviewed]

Development of resting spores on plants inoculated with a dikaryotic resting spore of Plasmodiophora brassicae
Narisawa; K. and Hashiba; T., A dikaryotic resting spore or two monokaryotic resting spores of Plasmodiophora brassicae were inoculated onto 1-d-old seedlings of Brassica campestris. The relative DNA content of a nucleus in a dikaryotic resting spore corresponded with that of a haploid nucleus in a monokaryotic resting spore. Plasmodia and zoosporangia were observed within the galls in some root cortical cells approximately 2 mo after inoculation. Resting spores were also formed within the galls in some cortical cells, and root hair infection was observed. The frequency of resting spore formation in B. campestris seedlings inoculated with a dikaryotic resting spore or two monokaryotic resting spores was 26.2 and 3.6%, respectively. The results indicate that the life-cycle of P. brassicae was completed on plants infected with a dikaryotic resting spore or by two monokaryotic resting spores., CAMBRIDGE UNIV PRESS
Mycol. Res, 1998, [Reviewed]

Suppression of Clubroot Formation in Chinese cabbage by Root endophytic Fungus, Heteroconium chaetospira
Narisawa; K.; Tokumasu; S. and Hashiba; T., Root-colonizing fungi were isolated from a total of 663 root segments of Chinese cabbage plants grown in soils collected from wheat, rape, Chinese cabbage, and napier grass fields. Most of the 322 isolates were from the wheat field soil and comprised 18 genera and two septate fungal groups. Hyaline and dark septate fungi accounted for approximately half the isolates from the wheat field soil. Sixteen isolates almost completely suppressed clubroot in sterile soil. Amongst these 16 isolates, two from Heteroconium chaetospira were also effective in nonsterile soil. Chinese cabbage seedlings from seed treated with these two isolates appeared healthy, and inoculation with one isolate promoted growth. Hyphae of the fungus covered the root surface and extensively colonized the inner cortical tissues., BLACKWELL SCIENCE LTD
Plant Pathology, 1998, [Reviewed]

Symbiotic association between Chinese cabbage and biocontrol endophytic fungus, Heteroconium chaetospira　　　　　　　　　　　　　　　
Narisawa; K. and Hashiba; T.
7th ICPP, 1998, [Reviewed]

Root endophytic fungus, Heteroconium chaetospira controls soilborne disease by colonizing into host plants　　　　　　　　　　　　　　　
Narisawa; K.; Yamada; K.; and Hashiba; T.
JMS-BMS Joint meeting, 1998, [Reviewed]

Biocontrol of clubroot disease of Chinese cabbage by root endophytic fungus, Heteroconium chaetospira.　　　　　　　　　　　　　　　
Narisawa; K.; and Hashiba; T.
農林水産省東北農業試験場公開国際シンポジウム「アブラナ科野菜の根こぶ病防除の現状と将来展望」, 1998, [Invited]

Biocontrol of Clubroot Disease in Brassica campestris by Root endophytic fungus, Heteroconium chaetospira　　　　　　　　　　　　　　　
Narisawa; K.; and Hashiba; T
Plant Growth-Promoting Rhizobacteria-Present Status and Future Prospects-, 1997, [Reviewed], [Invited]

The efficient root infection with single resting spores of Plasmodiophora brassicae
Narisawa; K.; Kageyama; K. and Hashiba; T., An efficient method of inoculation with single resting spores of Plasmodiophora brassicae has been developed. After purification of spore suspensions through step-wise density gradient centrifugation, the frequency of viable spores detected by staining with calcofluor white and ethidium bromide was ~ 80%. Approximately 90% of resting spores had one nucleus, the remainder were larger with two nuclei. Single resting spores which were small and considered to be viable were isolated under a microscope, and inoculated into 1-d-old seedlings of Brassica campestris by using the automatic system transferring single cells. A few small galls were observed on roots ~ 2 months after inoculation. Plasmodia were recognized mainly in galls on some roots. We concluded that the fungus grown in the host plants resulted in the formation of plasmodia. The frequency of successful infection with single resting spores was 48%.
Mycol. Res, 1996, [Reviewed]

Biological control of Plasmodiophora brassicae using rhizoplane fungi　　　　　　　　　　　　　　　
Narisawa; K.; and Hashiba; T.
13th IPPC, 1995, [Reviewed]

Axenic culture of the spermogonial state of Puccinia recondita f. sp. tritici
Narisawa; K.; Minakawa; H.; Yamaoka; Y. and Katsuya; K, Axenic cultures of Puccinia recondita f. sp. tritici were established by placing aseptic leaf segments with spermogonia on artificial media. Good growth of colonies was observed on the medium which contained 25% concentration of Murashige and Skoog's minerals, vitamins, and glycine; sucrose, 40 g; 2,4-D, 0.001 g; kinetin, 0.0001 g; peptic peptone, 4 g and bacto agar, 8 g per liter. The colonies of the axenic cultures were white to yellow and felty to fluffy in appearance and consisted of narrow and straight hyphae. Microscopic observation and determination of the DNA content of the nucleus in hyphal cells showed that the cultures were haploid and uninucleate., The Phytopathological Society of Japan (PSJ)
Ann. Phytopath. Soc. Japan, 1994, [Reviewed]

Mating type of isolates derived from the spermogonial state of Puccinia coronata var. coronata
Narisawa; K.; Yamaoka; Y. and Katsuya; K., Hyphal confrontation between two haploid cultures originating from single basidiospores was used to determine the mating type of Puccinia coronata var. coronata. Pairs of 15 single-basidiospore cultures were placed approximately 1 mm apart on the medium in all possible combinations. Hyphae of the pairs of colonies came into contact with each other in all combinations approximately two weeks after confrontations. When the nuclear number of hyphal cells in a contact zone was investigated one month after confrontation, monokaryotic hyphae were observed in selfing combination. On the other hand, dikaryotic hyphae were observed in 90.5% of crossing combinations between different cultures. Two isolates are considered compatible if dikaryotic hyphae are present in the contact zone but incompatible if they are absent. The mating type of the fungus was found to be characterized by multiple-allelomorphic tetrapolar incompatibility controlled by the “A” and “B” incompatible factors. © 1994, Mycological Society of Japan. All rights reserved.
Mycoscience, 1994, [Reviewed]

Root infection with a single resting spore of Plasmodiophora brassicae,
Narisawa; K.; and Hashiba; T., An efficient method of inoculation with single resting spores of Plasmodiophora brassicae has been developed. After purification of spore suspensions through step-wise density gradient centrifugation, the frequency of viable spores detected by staining with calcofluor white and ethidium bromide was ~ 80%. Approximately 90% of resting spores had one nucleus, the remainder were larger with two nuclei. Single resting spores which were small and considered to be viable were isolated under a microscope, and inoculated into 1-d-old seedlings of Brassica campestris by using the automatic system transferring single cells. A few small galls were observed on roots ~ 2 months after inoculation. Plasmodia were recognized mainly in galls on some roots. We concluded that the fungus grown in the host plants resulted in the formation of plasmodia. The frequency of successful infection with single resting spores was 48%.
5th IMC, 1994

Axenic Cultures of Spermogonial State of pussinia recondita f. sp. tritici Puccinia recondita f. sp. tritici　　　　　　　　　　　　　　　
NARISAWA,K; MINAKAWA,H; YAMAOKA,Y; KATSUYA,K, The Phytopathological Society of Japan (PSJ)
Annals of the Phytopathological Society of Japan, Jun. 1993

Establishment of axenic culture of Puccinia coronata var. coronata from basidiospores and their nuclear condition.
Narisawa; K.; Yamaoka; Y. and Katsuya; K., 日本菌学会
Trans. mycol. Soc. Japan, 1993, [Reviewed]

Nuclear Behavior in Germ Tubes of Basidiospores and Hyphae of Puccinia coronata var.coronata on an Artificial Medium　　　　　　　　　　　　　　　
NARISAWA,K; YAMAOKA,Y; KATSUYA,K, The Phytopathological Society of Japan (PSJ)
Annals of the Phytopathological Society of Japan, Oct. 1992

Axenic culture of the spermogonial and aecial state of Puccinia coronata var. coronata.　　　　　　　　　　　　　　　
Narisawa; K.; Yamaoka; Y. and Katsuya; K.
Trans. mycol. Soc. Japan, 1992, [Reviewed]

駒止湿原開墾跡地における土着微生物を用いたブナ植樹試験-植樹後5年の生育経過-
轟丈瑠; 郭永; 野口愛; 西澤智康; 成澤才彦; 坂上伸生
日本土壌肥料学会講演要旨集(Web), 2023

Environmental characteristics and application of symbiotic fungi for planting of beech seedlings in the abandoned cropland around Komado-Shitsugen
轟丈瑠; GUO Yong; 野口愛; 高島勇介; 西澤智康; 成澤才彦; 坂上伸生
日本生態学会大会講演要旨(Web), 2022

駒止湿原開墾跡地でのブナ林再生に向けた植樹試験における共生菌類の利用
轟丈瑠; 郭永; 西澤智康; 高島勇介; 渡邊眞紀子; 成澤才彦; 坂上伸生
日本土壌微生物学会大会講演要旨集(CD-ROM), 2021

駒止湿原開墾跡地におけるブナ共生菌類を利用した植樹試験の試行
轟丈瑠; 郭永; 高島勇介; 渡邊眞紀子; 西澤智康; 成澤才彦; 坂上伸生
日本土壌微生物学会大会講演要旨集(CD-ROM), 2020

高温ストレス条件下でのVeronaeopsis simplex Y34接種トマトのHeat Shock Proteinの発現状態
草谷奈津子; 晝間敬; 成澤才彦
日本土壌微生物学会大会講演要旨集(CD-ROM), 2020

Impacts on the Host Plants by Inoculation of Root Endophytes
堀淑恵; 成澤才彦; 東樹宏和
日本生態学会大会講演要旨(Web), 2020

Mortierella humilis/verticillata complexとその内生細菌のトマト生育への影響について
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本微生物生態学会大会(Web), 2019

根部エンドファイトVeronaeopsis simplex Y34のトマト根部微生物叢への時空間的な影響
野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本微生物生態学会大会(Web), 2019

Elucidation of Biological Interactions of Endohyphal Bacteria within Fungal Hyphae
西澤智康; 郭永; 高島勇介; 成澤才彦; 太田寛行
Institute for Fermentation, Osaka. Research Communications, 2019

根部エンドファイトVeronaeopsis simplex Y34の内外生細菌Rhizobium pusense Y9およびその近縁種が宿主菌類に及ぼす影響
橋本実佳; 郭永; 西澤智康; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

高温および有機態窒素条件におけるMortierella humilisとその内生細菌がトマト生育に及ぼす影響
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

Isolation and characterization of endophytic microbes associated with the pioneer grass colonizing on the recent volcanic deposits in the island of Miyake, Japan
孫寒; 西澤智康; 成澤才彦; 太田寛行
日本土壌微生物学会講演要旨集, 2019

Veronaeopsis simplex Y34接種トマトの根圏微生物叢の経時的モニタリング
野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

根部エンドファイトVeronaeopsis simplex Y34から分離されたRhizobium pusense Y9株とその近縁種が宿主に及ぼす影響
橋本実佳; 郭永; 西澤智康; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

Veronaeopsis simplex Y34資材を用いたハウス栽培におけるトマト根部微生物叢への影響
野口愛; 高田圭太; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

糸状菌内生細菌Mycoavidusの二次代謝産物生合成遺伝子の系統解析
郭永; SHARMIN Dilruba; 高島勇介; 成澤才彦; 太田寛行; 西澤智康
日本土壌微生物学会講演要旨集, 2018

Mortierella属菌とその内生細菌がトマト生育に及ぼす影響
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

平成27年9月関東・東北豪雨の影響を受けた農作物の無機元素組成に関する調査報告
坂上伸生; 齋藤明葉; 西澤智康; 成澤才彦; 伊藤哲司
日本地理学会発表要旨集, 2017

土壌微生物が創る共生の世界 －その先端的研究事例と農業への応用的研究展開4．糸状菌に共生する細菌その機能と生態－
太田寛行; 西澤智康; 松岡勇人; 高島勇介; 成澤才彦
日本土壌肥料学雑誌, Aug. 2016, [Reviewed], [Invited]

土壌糸状菌に内生するBurkholderiaceae科細菌の比較ゲノム解析
西澤智康; 藤村玲子; 大島翔子; 大島健志朗; 郭永; 佐藤嘉則; 服部正平; 服部正平; 成澤才彦; 太田寛行
日本土壌肥料学会講演要旨集, 09 Sep. 2015

刈敷を活用した不耕起・草生畑地の土壌微生物群集構造解析
雫田麻衣; 小松崎将一; 成澤才彦; 太田寛行; 西澤智康
土と微生物, 2015

不耕起・草生畑地土壌中に形成される土壌団粒の真核微生物群集構造解析
雫田麻衣; 中根麻冴美; 西澤智康; 西澤智康; 小松崎将一; 小松崎将一; 成澤才彦; 成澤才彦; 橋本知義; 太田寛行; 太田寛行
日本微生物生態学会大会(Web), 2015

根部エンドファイトVeronaeopsis simplexから分離されたRhizobium sp.Y9株の全ゲノム解析
松岡勇人; KHASTINI Rida; 郭永; 西澤智康; 成澤才彦
日本微生物生態学会大会(Web), 2015

糸状菌、特にエンドファイトの諸形質を内生細菌がコントロールするのか？
高島勇介; 太田寛行; 成澤才彦
土と微生物, 2015, [Invited]

P25-18 糸状菌Mortierella elongataに内生する新属新種細菌の性状解析(ポスター発表)
大島,翔子; 佐藤,嘉則; 藤村,玲子; 西村,歩; 西澤,智康; 成澤,才彦; 太田,寛行
日本微生物生態学会講演要旨集, 16 Oct. 2014

Fungal community in sclerotia of Japanese beech forest soils in northern Japan　　　　　　　　　　　　　　　
Amasya, A; Narisawa, K; Watanabe, M
European Geosciences Union General Assembly 2014, May 2014

内生細菌が糸状菌Moritierella elongataの性状に及ぼす影響(2013年度大会一般講演要旨)
西村 歩; 大島 翔子; 藤村 玲子; 佐藤 嘉則; 大島 健志郎; 服部 正平; 成澤 才彦; 太田 寛行
土と微生物, 2014

PB-039 Pythium aphanidermatum及びP. nunnに内生する細菌様構造物の検出(PB 共生/相互作用,ポスター発表)
高島,勇介; 佐藤,嘉則; 東條,元昭; 成澤,才彦
日本微生物生態学会講演要旨集, 08 Oct. 2011

糸状菌細胞に内生する細菌の存在とその検出法
佐藤嘉則; 成澤才彦; 西澤智康; 小松﨑将一; 太田寛行
土と微生物, 2011

1A-14 Characterization of endobacterial communities in dark-sepate endophytic fungus, Veronaeopsis simplex associated with biocontrol mechanisms of Fusarium disease of Chinese Cabbage(Oral Session) :
KHASTINI,RIDA; SATO,YOSHINORI; NISHIZAWA,TOMOYASU; OHTA,HIROYUKI; NARISAWA,KAZUHIKO
23 Nov. 2010

Effect of sowing and harvest date on sugar yield of Sweet sorghum(Sorghum bicolor (L.) Moench).
Nitta, Y; Umehara, R; Kamiyama, A; Kobayashi, A; Matsuda, T; Inoue, E; Narisawa, K; Kurusu, Y; Ohta, H; Chonan, S; Miyaguchi, Y; Toyoda, A; Kato, T; Kobayashi, H; Komatsuzaki; M. Sato, T; Nakamura, S; Goto, Y
Japanese Journal of Crop Science, 2009

Annual Differences of Sugar Production in Sweet Sorghum (Sorghum bicolor (L.) Moench) Stem
KAMIYAMA Akinori; NARISAWA Kazuhiko; KURUSU Yasurou; OHTA Hiroyuki; CHOHNAN Shigeru; MIYAGUCHI Yuji; TOYODA Atsushi; KATO Tasuku; KOBAYASHI Hisashi; KOMATSUZAKI Masakazu; SATO Tatsuo; NITTA Youji; YOSHIDA Koushi; HOMMA Takashi; UMEHARA Ryouhei; KOBAYASI Ryo; MATSUDA Toshiaki; NAKAMURA Satoshi; GOTO Yusuke; INOUE Eiichi
Tohoku Journal of Crop Science, 2009

Effect of Weather Condition on Sugar Yield in Sweet Sorghum (Sorghum bicolor (L.) Moench) Stem
KAMIYAMA Akinori; NARISAWA Kazuhiko; KURUSU Yasurou; OHTA Hiroyuki; CHOHNAN Shigeru; MIYAGUCHI Yuji; TOYODA Atsushi; KATO Tasuku; KOBAYASHI Hisashi; KOMATSUZAKI Masakazu; SATO Tatsuo; NITTA Youji; YOSHIDA Koushi; HOMMA Takashi; UMEHARA Ryouhei; KOBAYASI Ryo; MATSUDA Toshiaki; NAKAMURA Satoshi; GOTO Yusuke; INOUE Eiichi
Tohoku Journal of Crop Science, 2009

06-104 Effect of organic fertilizer inputs on nitrous oxide emission and microbial community in upland soils(Biogeochemical cycles)
Umezu,Masafumi; Sato,Yoshinori; Nishizawa,Tomoyasu; Niimi,Hiroshi; Hashimoto,Tomoyoshi; Narisawa,Kazuhiko; Komatsuzaki,Masakazu; Ohta,Hiroyuki
日本微生物生態学会講演要旨集, 25 Nov. 2008

Sweet sorghum cultivation as bio-fuel crop in Ibaraki prefecture.
Nitta, Y; Kamiyama, A; Matsuda, T; Nakamura, S; Goto, Y; Inoue, E; Narisawa, K; Kurusu, Y; Ohta, H; Chonan, S; Toyoda, A; Kato, T; Kobayashi, H; Komatsuzaki, M; Sato, T
Japanese Journal of Crop Science 77, 2008

有機物連用土壌における亜酸化窒素発生に関わる微生物群集構造の解析
梅津昌史; 佐藤嘉則; 西澤智康; 新美洋; 橋本知義; 成澤才彦; 小松崎将一; 太田寛行
日本微生物生態学会講演要旨集, 2008

PA-57 Developing factors of sclerotia-like structures formed by some dark septate fungal isolates from sclerotium grain and acid forest soil(Physiology/Growth,Poster presentation A) :
Nonoyama Yaya; Narisawa Kazuhiko; Ohta Hiroyuki; Watanabe Makiko
日本微生物生態学会講演要旨集, 2007

根部エンドファイトの共生および病害抑制メカニズム　　　　　　　　　　　　　　　
羽柴輝良; 森田聡一郎; 成澤才彦; 薄史曉
日本農芸化学会誌, 2003

なぜエンドファイトの感染は病徴を示さないのか　　　　　　　　　　　　　　　
成澤才彦
化学と生物, 2000

植物と相利共生するエンドファイト
大木健広; 成澤才彦; 羽柴輝良
植物の化学調節, 1999

根部エンドファイトを利用した土壌病害防除の実現に向けて
成澤才彦
農業技術, 1999

根部エンドファイトによるハクサイ根こぶ病の防除　　　　　　　　　　　　　　　
成澤才彦
農業茨城, 1999

新しい資源を生み出す宝庫ー植物と相利共生する微生物-３エンドファイト　　　　　　　　　　　　　　　
羽柴輝良; 成澤才彦; 島貫忠幸
化学と生物, 1998

Heteroconium chaetospiraの土壌病害防除への利用　　　　　　　　　　　　　　　
成澤才彦; 羽柴輝良
BRAIN TECHNO NEWS, 1998

根部エンドファイトによるハクサイ根こぶ病の防除
成澤才彦
農業技術体系, 1998

根部エンドファイトによるハクサイ根こぶ病の防除
成澤才彦
農耕と園芸, 1998

ハクサイの根こぶ病・黄化病を抑制する根部エンドファイト
成澤才彦; 羽柴輝良
化学と生物, 1997

根部エンドファイトの利用―微生物利用の新たな可能性 みんなの有機農業技術大事典
農山漁村文化協会, Joint work
農山漁村文化協会, Mar. 2025
9784540241079

有機農業への根部エンドファイトの活用−イチゴ栽培を例に−　　　　　　　　　　　　　　　
成澤才彦
JATAFFジャーナル, Dec. 2024

〔Major achievements〕もっと菌根の世界,知られざる根圏のパートナーシップ　　　　　　　　　　　　　　　
成澤才彦, Joint work
築地書館, 22 Sep. 2023
9784806716068

〔Major achievements〕Investigating endobacteria that thrive within Mucoromycota. In: Martin F, Uroz S (Eds). Microbial Environmental Genomics (MEG), Methods in Molecular Biology,　　　　　　　　　　　　　　　
Alessandro Desirò; Yusuke Takashima; Gregory Bonito; Tomoyasu Nishizawa; Kazuhiko Narisawa; Paola Bonfante, Joint work
Springer, Dec. 2022, [Reviewed]
9781071628713

Fungal communities of Sclerotia grains from forest soils., In Watanabe, M. (Ed.), Sclerotia grains in soils: Anew perspective from pedosclerotiology. Progress in soil science　　　　　　　　　　　　　　　
Narisawa; K.; Amasya; A.; Sasaki Nonoyama; Y. and Obase; K., Contributor
Springer Singapore, Feb. 2021
9789813342514

Relationships soil mesofauna, ectomycorrhizal fungi, and sclerotia in forest soils., In Watanabe, M. (Ed.), Sclerotia grains in soils: Anew perspective from pedosclerotiology. Progress in soil science　　　　　　　　　　　　　　　
Amasya; A. and Narisawa; K., Contributor
Springer Singapore, Feb. 2021
9789813342514

Sclerotia grains as bacterial carriers in soil., In Watanabe, M. (Ed.), Sclerotia grains in soils: Anew perspective from pedosclerotiology. Progress in soil science　　　　　　　　　　　　　　　
Sasaki Nonoyama; Y. and Narisawa; K., Contributor
Springer Singapore, Feb. 2021
9789813342514

植物病理学 第２版　　　　　　　　　　　　　　　
眞山滋志、土佐幸雄, Contributor
文永堂出版, 01 Mar. 2020
9784830041389

植物共生菌による省資源型栽培「有機農業大全」−持続可能な食の技術と思想−　　　　　　　　　　　　　　　
成澤才彦, Contributor
コモンズ, 20 Dec. 2019
9784861871641

農学入門　　　　　　　　　　　　　　　
太田寛行; 成澤才彦, Joint work
養賢社, Sep. 2013
9784842505190

Isolation and selection of fungal endophytes for the suppression of soil-borne diseases, In: Prospects and Applications for Plant-Associated Microbes: A laboratory Manual.　　　　　　　　　　　　　　　
Narisawa; K.; and Diene; O., Joint work
BioBien Innovations, Paimio, Finland, 2011

Evaluation of fungal endophytes for the control of plant diseases, In: Prospects and Applications for Plant-Associated Microbes: A laboratory Manual.　　　　　　　　　　　　　　　
Narisawa; K.; and Diene; O, Joint work
BioBien Innovations, Paimio, Finland, 2011

エンドファイトの働きと使い方　　　　　　　　　　　　　　　
成澤才彦, Single work
農文協, 2011

内生菌類による誘導抵抗-誘導抵抗による生物防除- 「微生物と植物の相互作用-病害と生物防除-」　　　　　　　　　　　　　　　
成澤才彦, Joint work
ソフトサイエンス社, 2009

農業と微生物-農作物と共生する微生物たち- 「微生物ってなに？」　　　　　　　　　　　　　　　
成澤才彦, Single work
日科技連出版社, 2006

菌類エンドファイト－植物との相互作用とその利用「作物保護の新展開－バイオサイエンスのかけはし－」　　　　　　　　　　　　　　　
成澤才彦, Single work
ソフトサイエンス社, 2005

根部エンドファイトHeteroconium chaetospiraによるハクサイ根こぶ病の防除－ 「作物保護の新展開－バイオサイエンスのかけはし－」　　　　　　　　　　　　　　　
薄史曉; 成澤才彦; 羽柴輝良, Joint work
ソフトサイエンス社, 2005

根部エンドファイトによるハクサイ根こぶ病の防除「拮抗微生物による作物病害の生物防除－我が国における研究事例・実用化事例－」　　　　　　　　　　　　　　　
成澤才彦; 薄史曉, Joint work
日本印刷工業株式会社, 2003

微生物農薬－さまざまな分野での応用技術－「微生物の取り扱いと利用・応用技術」　　　　　　　　　　　　　　　
成澤才彦, Single work
情報機構, 2003

ハクサイ根こぶ病菌・Plasmodiophora brassicae の病徴発現について-植物感染生理学研究の現状と将来展望-　　　　　　　　　　　　　　　
成澤才彦; 羽柴輝良, Joint work
仙台共同印刷, 1996

イチゴ炭疽病を抑制するDark septate endophytesの選抜　　　　　　　　　　　　　　　
岡根慧美; 成澤才彦
日本有機農業学会 第25回東京大会, Dec. 2024

高温環境下におけるハクサイの生育に有効なDark Septate Endophytes (DSE)の探索．　　　　　　　　　　　　　　　
髙井雅子; 野口愛; I Gede Karta; Satria Wibawa; 大坪華蓮; 成澤才彦
日本微生物生態学会 第37回広島大会, Oct. 2024

A Newly Discovered Dark Septate Fungus Cladophialophora sp. FX2: Exploring Its Endophytic and Ecological Behaviour　　　　　　　　　　　　　　　
Felix; 成澤才彦
日本微生物生態学会 第37回広島大会, Oct. 2024

Mortierella humilis S2の農業利用の可能性　　　　　　　　　　　　　　　
及川慶太; 坂上伸生; 成澤才彦
日本微生物生態学会 第37回広島大会, Oct. 2024

気候変動適応および肥料削減を目的としたテンサイ栽培における根部エンドファイトの利用可能性について　　　　　　　　　　　　　　　
熊本光太郎; 野口愛; 坂上伸生; Wibawa IGKS; 及川慶太; 須之内真穂; 浅木直美; 成澤才彦
日本土壌微生物学会2024年度大会, Jun. 2024

低温環境下でキュウリおよびトマトの生育を促進するDark Septate Endophytes の選抜　　　　　　　　　　　　　　　
出口陽菜; 成澤才彦
日本菌学会関東支部 令和6年度年次大会, 20 Apr. 2024

子嚢菌類に内生または外生する細菌の検出　　　　　　　　　　　　　　　
大坪華蓮; 成澤才彦
日本菌学会関東支部 令和6年度年次大会, 20 Apr. 2024

畑土壌に存在するMortierella属菌がトマトの生育およびリン酸吸収に与える影響　　　　　　　　　　　　　　　
及川慶太、成澤才彦
日本有機農業学会 第24回大会, 09 Dec. 2023, 日本有機農業学会

ゴボウの土壌病害には種子由来の菌類が影響する！－エンドファイトでの防除の取り組み－　　　　　　　　　　　　　　　
佐藤佑飛、成澤才彦
日本有機農業学会 第24回大会, 09 Dec. 2023, 日本有機農業学会

農作物への重金属の蓄積をエンドファイトは制御できるのか？　　　　　　　　　　　　　　　
穂積由樹、成澤才彦
日本有機農業学会 第24回大会, 09 Dec. 2023, 日本有機農業学会

Emerging the Elusive Symbiotic Function of Dark Septate Endophyte Cladophialophora chaetospira SK51 in Strawberry, a Perennial SD Plant: New Attempt for Flowering and Fruiting Behavior　　　　　　　　　　　　　　　
Wiwiek Harsonowati and Kazuhiko Narisawa
The First International Conference on Food and Agricultural Sciences (ICFAS), Nov. 2022, [Invited]

Mortierella属菌およびその内生細菌が各種植物の生育に与える影響　　　　　　　　　　　　　　　
坂本瑞希、成澤才彦
日本微生物生態学会 第35回大会, Oct. 2022, 日本微生物生態学会

Cladophialophora sp.13nは乾燥ストレス条件下でトウモロコシの生育を促進する　　　　　　　　　　　　　　　
森田翔央、成澤才彦
日本微生物生態学会 第35回大会, Oct. 2022, 日本微生物生態学会

Mortierella sugadairana に細胞内共生するBurkholderiaceae未記載種のゲノム解析　　　　　　　　　　　　　　　
高島勇介,郭永,吉橋佑馬,出川洋介,西澤智康,成澤才彦,津田吉晃
日本微生物生態学会第35回大会, Oct. 2022, 日本微生物生態学会

Screening of root endophytes to promote maize growth under stress conditions.　　　　　　　　　　　　　　　
Haoyue Lu; Kazuhiko Narisawa
日本土壌微生物学会 2022年度大会, Jun. 2022

茨城県におけるテンサイの生育と共生菌類の接種による耐暑性付与　　　　　　　　　　　　　　　
日本土壌微生物学会 2022年度大会, Jun. 2022, 日本土壌微生物学会

Dark Septate Endophytesのイネ生育への影響について　　　　　　　　　　　　　　　
関根直人,成澤才彦
日本有機農業学会 2021年度大会, 04 Dec. 2021

ロボット芝刈機と根部エンドファイトを利用したミニトマトの新しい不耕起草生・有機栽培技術の検討　　　　　　　　　　　　　　　
福田 真丈; 高嶋 尚哉; 野口 愛; 成澤 才彦; 坂上 伸生; 小松﨑 将一
第79回農業食料工学会, 13 Sep. 2021

トマト苗への根部エンドファイトVeronaeopsis simplex接種が土壌化学性および植物体元素組成に及ぼす影響　　　　　　　　　　　　　　　
大好優華・助川歩乃佳・野口 愛・Wibawa IGKS・松浦江里・高田圭太・成澤才彦・坂上伸生
日本土壌微生物学会2021年度大会, 18 Jun. 2021

有機農法水田に自生する植物に定着する菌類叢の解析　　　　　　　　　　　　　　　
関根直人、成澤才彦
日本土壌微生物学会 2020年度大会, 15 Jun. 2020

高温ストレス条件下でのVeronaeopsis simplex Y34接種トマトのHeat Shock,Proteinの発現状態　　　　　　　　　　　　　　　
草谷奈津子、晝間敬、成澤才彦
日本土壌微生物学会 2020年度大会, 15 Jun. 2020

Mortierella humilis/ verticillata complexとその内生細菌のトマト生育への影響について　　　　　　　　　　　　　　　
松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
日本微生物生態学会第33回大会, 11 Sep. 2019

Mortierella 属糸状菌に内生するMycoavidus属細菌のゲノム縮小化　　　　　　　　　　　　　　　
郭 永、高島勇介、Dilruba Sharmin、佐藤嘉則、成澤才彦、太田寛行、西澤智康
日本土壌微生物学会2019年度大会, 15 Jun. 2019

根部エンドファイトVeronaeopsis simplex Y34の内外生細菌Rhizobium pusense Y9　　　　　　　　　　　　　　　
橋本実佳、郭永、西澤智康、成澤才彦
日本土壌微生物学会2019年度大会, 15 Jun. 2019

ストレス条件下において根部エンドファイトVeronaeopsis simplexY34がエンドウ生育に及ぼす影響　　　　　　　　　　　　　　　
山崎亜耶乃、成澤才彦
日本土壌微生物学会 2019年度大会, 15 Jun. 2019

小笠原母島から分離されたSaksenaea属1未記載種およびそのBurkholderia属内生細菌について　　　　　　　　　　　　　　　
高島勇介; 西澤智康; 太田寛行; 成澤才彦
平成31年度日本菌学会関東支部年次大会, 20 Apr. 2019

Correlations between vegetation and soil microbiome across a vegetation gradient on the deforested land adjacent to the Komado-Shitsugen moor in Japan.　　　　　　　　　　　　　　　
Guo Y; Sakagami N; Takashima Y; Nishizawa T; Narisawa K; Watanabe M.
17th World Lake Conference, 18 Oct. 2018

駒止湿原開墾跡地におけるブナ生育に関わる共生菌類の探索と植樹試験への利用　　　　　　　　　　　　　　　
猪瀬有美、高島勇介、郭永、坂上伸生、西澤智康、渡邊眞紀子、成澤才彦
第17回世界湖沼会議, 18 Oct. 2018

Veronaeopsis simplex Y34資材を用いたハウス栽培におけるトマト根部微生物叢への影響　　　　　　　　　　　　　　　
野口愛、高田圭太、坂上伸生、郭永、西澤智康、太田寛行、成澤才彦
日本土壌微生物学会2018年度大会, 17 Jun. 2018

Mortierella属菌とその内生細菌がトマト生育に及ぼす影響　　　　　　　　　　　　　　　
松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
日本土壌微生物学会2018年度大会, 16 Jun. 2018

Veronaeopsis simplex Y34 から分離された Rhizobium pusense Y9 株とその近縁種が宿主に及ぼす影響　　　　　　　　　　　　　　　
橋本実佳、郭永、西澤智康、成澤才彦
日本土壌微生物学会2018年度大会, 16 Jun. 2018

根部エンドファイトVeronaeopsis simplexY34を用いたFusarium属菌によるエンドウ土壌病害の抑制　　　　　　　　　　　　　　　
山崎亜耶乃、成澤才彦
日本土壌微生物学会 2018年度大会, 16 Jun. 2018

駒止湿原開墾跡地のブナ生育に関わる共生菌類の探索　　　　　　　　　　　　　　　
猪瀬有美; 郭永; 高島勇介; 坂上伸生; 渡邊眞紀子; 成澤才彦
菌根研究会2017年度大会, Dec. 2017

共に生きる菌類と細菌—内生細菌は宿主菌類をどう変えるのか?−　　　　　　　　　　　　　　　
高島勇介; 成澤才彦
環境微生物系学会合同大会2017 企画シンポジウムS06:共生微生物—我々の理解はどこまで進んだのか?, Aug. 2017

An EHB Mycoavidus cysteinexigens enhances in vitro carbon utilization by host Mortierella elongata　　　　　　　　　　　　　　　
郭永; 小沼奈那美; 高島勇介; Dilruba Sharmin; 佐藤嘉則; 成澤才彦; 真鍋理一郎; 高島昌子; 大熊盛也; 太田寛行; 西澤智康
環境微生物系学会合同大会2017, Aug. 2017

駒止湿原開墾跡地の植生回復を目的としたブナ外生菌根部からの菌類の獲得　　　　　　　　　　　　　　　
猪瀬有美; 郭永; 高島勇介; 坂上伸生; 渡邊眞紀子; 成澤才彦
環境微生物系学会合同大会2017, Aug. 2017

Isolation and selection of cellulolytic dark septate endophytic fungi that able to promote asparagus plant growth.　　　　　　　　　　　　　　　
Surono and Kazuhiko Narisawa
The 7th Japan-Taiwan-Korea International Symposium on Microbial Ecology, 19 Oct. 2016

Mollicutes-related endobacteriaはケカビ亜門菌類3目すべてに内生する　　　　　　　　　　　　　　　
高島 勇介; 成澤 才彦
2015年度菌根研究会大会, 31 Oct. 2015

グロムス門およびケカビ亜門菌類に内生するバクテリアの多様性　　　　　　　　　　　　　　　
高島 勇介; 成澤 才彦
日本微生物生態学会第30回大会, 20 Oct. 2015

根部エンドファイトVeronaeopsis simplexから分離されたRhizobium sp. Y9株の全ゲノム解析　　　　　　　　　　　　　　　
松岡 勇人; Rida Khastini; 西澤 智康; 成澤 才彦
日本微生物生態学会第30回大会, 19 Oct. 2015

ラン菌根菌は内生バクテリアを保持するのか　　　　　　　　　　　　　　　
中西布実子; 成澤 才彦
日本微生物生態学会第30回大会, 19 Oct. 2015

Detection of Mollicutes- related endobacteria from putative saprotrophic Endogone spp. and Sphaerocreas pubescens　　　　　　　　　　　　　　　
TAKASHIMA, Y; K. Yamamoto; K. Seto; Y. Degawa; K. Narisawa
The 8th International Conference on Mycorrhiza (ICOM8), 05 Aug. 2015

ケカビ目菌類から検出されたBurkholderia属細菌について　　　　　　　　　　　　　　　
高島勇介; 伊藤英臣; 菊池義智; 広瀬大; 成澤才彦
日本土壌微生物学会2015年度大会, 22 May 2015

Endogone属菌およびSphaerocreas pubescensにおけるMollicutes-related endobacteriaの検出　　　　　　　　　　　　　　　
高島勇介; 山本航平; 瀬戸健介; 出川洋介; 成澤才彦
日本菌学会第59回大会, 16 May 2015

塩害土壌におけるトマト栽培へのPhialocephala helveticaの利用　　　　　　　　　　　　　　　
臼井絵里香; 菊地聖永; 成澤才彦
日本菌学会第59回大会, 16 May 2015

根部エンドファイトVeronaeopsis simplexに内生するバクテリアについて　　　　　　　　　　　　　　　
松岡 勇人; Rida Khastini; 成澤 才彦
日本菌学会第59回大会, 16 May 2015

菌類子実体内部から分離されたBurkholderia属菌について　　　　　　　　　　　　　　　
中西布実子; 成澤 才彦
日本菌学会第59回大会, 16 May 2015

根部エンドファイトPhialocephala fortinii BPC3によるトマトへのCs-137移行の抑制　　　　　　　　　　　　　　　
丸山剛; 坂上伸生; 小松崎将一; 成澤才彦
日本菌学会第59回大会, 16 May 2015

植物根部エンドファイトVeranoeaopsis simplex とその菌糸圏から分離されたバクテリアの相互作用　　　　　　　　　　　　　　　
松岡 勇人; Rida Khastini; 成澤 才彦
環境微生物系学会合同大会, 24 Oct. 2014

接合菌類および子嚢菌類における菌類内生バクテリアの検出率について　　　　　　　　　　　　　　　
高島 勇介; 出川 洋介; 成澤 才彦
環境微生物系合同大会, 24 Oct. 2014

子実体起源 Pleosporales sp.およびMortierella sp.に 内生するバクテリアについて　　　　　　　　　　　　　　　
中西布実子; 高島勇介; 太田寛行; 成澤才彦
環境微生物系学会合同大会, 24 Oct. 2014

The fungal endophytes Cladophialophora sp. and Phialocephala helvetica which promote growth of tomato plant under salinity stressed conditions.　　　　　　　　　　　　　　　
Erika Usui; Kazuhiko Narisawa
International Society for Environmental Biogeochemistry, 2013

Selection of Dark-septate endophytic (DSE) fungus associate with the roots of Spiranthes sinensis.　　　　　　　　　　　　　　　
Saki Shingaki; Kazuhiko Narisawa
31st New Phytologist Symposium Orchid Symbiosis, 2013

スイートソルガム連作障害の原因解明とVeronaeopsis simplex Y34を利用した同病害抑制について　　　　　　　　　　　　　　　
山本翔也; 小松崎佑樹; 塩津文隆; 成澤才彦
第29回日本微生物生態学会大会, 2013

根部エンドファイトVeronaeopsis simplex Y34による高温条件下でのトマトの生育促進及び萎凋病の抑制　　　　　　　　　　　　　　　
寺田晴菜; 成澤才彦
第29回日本微生物生態学会大会, 2013

根部エンドファイトPhialocephala fortiniiを用いたアスパラガス育苗法の有効性　　　　　　　　　　　　　　　
松岡 勇人; 菊池 聖永; 畑 明李; 成 澤 才彦
第29回日本微生物生態学会大会, 2013

ピーマンへ耐塩性付与効果を示す根部エンドファイト2種Phialocephala helveticaおよびCladophialophora sp.について　　　　　　　　　　　　　　　
臼井絵里香; 成澤才彦
日本菌学会第56回大会, 2012

Cunninghamella elegansに内生するBurkholderia属細菌について　　　　　　　　　　　　　　　
高島勇介; 成澤才彦
菌学会第56回大会, 2012

Mortierella属菌の保有する内生細菌の地理的分布について　　　　　　　　　　　　　　　
親泊貴志; 高島勇介; 成澤才彦
日本菌学会第56回大会, 2012

低酸素条件における根部エンドファイトVeronaeopsis simplex Y34 株の植物生育促進効果　　　　　　　　　　　　　　　
小松崎佑樹; 成澤才彦
第28回日本微生物生態学会大会, 2012

Colonization by the root endophytic fungus Camposporium indicum within roots of Rhododendron mucronatum　　　　　　　　　　　　　　　
皆川源一郎; 成澤才彦
第28回日本微生物生態学会大会, 2012

Assessment of Veronaeopsis simplex in plant growth promotion and Fusarium disease suppression on Chinese cabbage in vitro.　　　　　　　　　　　　　　　
Rida O. Khastini; Kazuhiko Narisawa; Hiroyuki Ohta
XIII International Congress of Bacteriology and Applied Microbiology, 2011

アスパラガス栽培への共生菌利用の試み−生育促進と病害抑制を目指して−　　　　　　　　　　　　　　　
成澤才彦
平成22年度 園芸学会春季大会 アスパラガス研究小集会, 2010, [Invited]

共生菌を利用したサスティナブルな燃料作物栽培への試み　　　　　　　　　　　　　　　
成澤才彦
日本作物学会関東支部第98回講演会, 2009, [Invited]

エンドファイトを利用したアスパラガス土壌病害耐性付与の試み　　　　　　　　　　　　　　　
成澤才彦
平成21年度 野菜茶業課題別研究会「アスパラガス連作障害発生要因解明の現状と対策技術の開発方向」, 2009, [Invited]

内生糸状菌利用の最前線　　　　　　　　　　　　　　　
成澤才彦
日本植物病理学会第10回記念バイオコントロール研究会, May 2007, 日本植物病理学会バイオコントロール研究会, [Invited]

菌類の名前を知ること－なぜこのシンポジウムを考えたのか　　　　　　　　　　　　　　　
成澤才彦
日本菌学会第５１回大会公開シンポジウム, May 2007, 日本菌学会, [Invited]

トマト根腐萎凋病を抑制するroot-associated fungus Meliniomyces variabilis (formerly named Variable White Taxon)　　　　　　　　　　　　　　　
成澤才彦; 大高伸明
日本菌学会第50回大会, 2006

育苗培土へのアミノ酸添加によるDark-septate endophytic fungusのメロン苗への定着率およびつる割病防除効果の向上　　　　　　　　　　　　　　　
成澤才彦; 小河原孝司; 大高伸明; 羽柴輝良
日本植物病理学会2005年大会, 2005

土壌pHおよび添加するアミノ酸がHeteroconium chaetospiraのハクサイ根部への定着に与える影響　　　　　　　　　　　　　　　
薄 史曉; 成澤才彦
日本菌学会第49回大会, 2005

根部エンドファイト－作物共生系の病害防除への利用　　　　　　　　　　　　　　　
成澤才彦
日本植物病理学会東北部会40周年記念シンポジウム, 2004, [Invited]

メロンつる割病を抑制するDark-septate endophytic fungus JAnPC9　　　　　　　　　　　　　　　
成澤才彦; 小河原孝司; 大高伸明; 羽柴輝良
日本植物病理学会2004年度大会, 2004

2014, 日本土壌微生物学会

2010, 日本有機農業学会

日本菌学会

日本植物病理学会

日本微生物生態学会

イチゴの花芽形成を誘導するエンドファイト－バクテリア共生系の利用技術の開発
基盤研究(B)
茨城大学
01 Apr. 2024 - 31 Mar. 2027

エンドファイトがストレス環境下でダイズの根粒形成を誘導する！
挑戦的研究(萌芽)
茨城大学
28 Jun. 2024 - 31 Mar. 2026

Is dark-septate endophytic fungi the key of the tripartite symbiotic system of plant-DSE-bacteria?
Grant-in-Aid for Scientific Research (C)
Ibaraki University
01 Apr. 2023 - 31 Mar. 2026

〔Major achievements〕Mortierella属菌の植物共生を制御する内生細菌の機能解明とその利用　　　　　　　　　　　　　　　
新学術領域研究(研究領域提案型)
Apr. 2022 - Mar. 2024

〔Major achievements〕エンドファイト−土壌微生物共生系の機能を活 用した気候変動に適応する持続的なテンサイ 栽培技術の確立　　　　　　　　　　　　　　　
基礎研究ステージ（基礎研究 型）
Apr. 2022 - Mar. 2024

〔Major achievements〕エンドファイトと土着微生物との共生系を利用すると安定して土壌病害を抑制できる　　　　　　　　　　　　　　　
基盤研究(B)
Apr. 2021 - Mar. 2024

〔Major achievements〕エンドファイト−土壌微生物共生系の機能を活 用した気候変動に適応する持続的なテンサイ 栽培技術の確立　　　　　　　　　　　　　　　
基礎研究ステージ（チャレンジ 型）
Apr. 2021 - Mar. 2022

内生細菌が腐生性Mortierella属菌に共生能を付与するメカニズムの解明
Challenging Research (Exploratory)
Ibaraki University
Jul. 2020 - Mar. 2022

内生細菌の働きを理解するとMortierella属菌が作物生産へ利用できる
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
茨城大学
Apr. 2020 - Mar. 2022

内生細菌を操ることで腐生菌であるMortierella属菌を共生菌に変える！
Challenging Research (Exploratory)
Ibaraki University
Jun. 2018 - Mar. 2020

植物−エンドファイト−バクテリア共生系による新たな生物学的窒素固定技術の確立　　　　　　　　　　　　　　　
基盤研究(B)
Apr. 2017 - Mar. 2020

エンドファイトDSEを利用したセシウムフリーおよび塩類耐性高糖度トマト栽培技術の開発
パイオニアエコサイエンス株式会社
2014

植物-エンドファイト共生系を利用した農作物へのセシウム吸収低減技術の確立
茨城大学
2012 - 2013

特許第7653209号, JP2023040453, マメ科植物用培土及びその使用、マメ科植物用栽培セット、マメ科植物の栽培方法、並びに、培土付きマメ科植物の苗
成澤 一彦, ニ ルウ プツ チトラ イノセンシア

特許第7529253号, 特開2021-052745, 特願2020-157666, ヒユ科の苗およびその生産方法
阿部 美聡, 水島 邦具, 成澤 一彦

特開2024-043828, 特願2022-149029, ヒユ科の苗、その生産方法、及び、菌の培養材
阿部 美聡, 石黒 成紀, 馬場 大輔, 成澤 一彦

特許第7430960号, JP2022043068, 植物の培土、培土を含む栽培セット、培土を用いた栽培方法、及び、培土付き植物の苗
成澤 一彦, ウイウイック ハルソノワティ

PCT/JP2022/43068, 植物の培土、培土を含む栽培セット、培土を用いた栽培方法、及び、培土付き植物の苗
成澤才彦、Wiwiek Harsonowati

特願2022-181348号, マメ科植物用培土及びその使用、マメ科植物用栽培セット、マメ科植物用の栽培方法、並びに、培土付きマメ科植物の苗
成澤才彦、Ni Luh Putu Citra Innosensia

特許第6898637号, 特開2018-174708, 特願2017-073441, 高機能性成分含有植物の育成方法
井上 良恵, 成澤 一彦

特開2021-052745, 特願2020-157666, ヒユ科の苗およびその生産方法
阿部 美聡, 水島 邦具, 成澤 一彦

特開2021-052740, 特願2020-145781, 培養材、培養土、植物の苗、および植物の栽培方法
阿部 美聡, 石黒 成紀, 辻 真輝, 水島 邦具, 成澤 一彦

特開2021-052741, 特願2020-145783, 培養材の製造方法、植物の栽培方法、培養土、および苗
阿部 美聡, 石黒 成紀, 辻 真輝, 水島 邦具, 成澤 一彦

特開2019-151609, 特願2018-039988, 植物の成長促進用組成物およびその利用
枳穀 豊, 中川 弘之, 成澤 一彦

特開2019-151608, 特願2018-039986, 植物の成長促進用組成物およびその利用
枳穀 豊, 中川 弘之, 成澤 一彦

特許第6497720号, 特願2018-039986, 植物の成長促進用組成物およびその利用
枳穀 豊, 中川 弘之, 成澤 一彦

特許第6499340号, 特願2018-039988, 植物の成長促進用組成物およびその利用
枳穀 豊, 中川 弘之, 成澤 一彦

特開2018-174708, 特願2017-073441, 高機能性成分含有植物の育成方法
井上 良恵, 成澤 一彦

特願2014-185444号, 根部エンドファイト（ＤＳＥ）Ｖｅｒｏｎａｅｏｐｓｉｓ ｓｉｍｐｌｅｘを利用したトマト苗の生産方法
成澤才彦、竹下達夫

US6,306,390 B1, Root endophyte having soil disease inhibitory activity, process for preparing said root endophyte, and method for inhibiting soil disease

特許第2801164号, ハクサイ土壌病害抑制能を有する根面菌、同根面菌の製造方法及びハクサイ土壌病害抑制方法

特願2011-182330号, 根部エンドファイト(DSE)Ｐｈｉａｌｏｃｅｐｈａｌａ ｆｏｒｔｉｎｉｉを利用したアスパラガス苗の生産方法

アグリビジネス創出フェア2023 出展者プレゼンテーション「エンドファイトがイチゴの花を咲かせる！？」　　　　　　　　　　　　　　
lecturer
21 Nov. 2023

植物バイオ研究会・Food Bio Plus研究会 公開講演会 「土壌微生物を活用した持続可能な食料生産」 「エンドファイトがイチゴに花を咲かせる！？」　　　　　　　　　　　　　　
lecturer
(一財)バイオインダストリー協会, 25 Oct. 2023

共生菌使い作物すくすく 茨城大学発新興、接種の培土と苗　　　　　　　　　　　　　　
appearance
日本経済新聞, 28 Sep. 2023

やさしい生態学講座「共に生きるとこんなことができる！ 微生物と植物を例に」　　　　　　　　　　　　　　
lecturer
国立科学博物館附属自然教育園, 19 Feb. 2023