ジネンジョ「稲武2号」のカルシウムとマグネシウムの吸収特性　　　　　　　　　　　　　　　
中村嘉孝; 田中哲司; 大竹敏也; 井上栄一, Last
愛知県農業総合試験場研究報告, 20 Dec. 2024, [Reviewed]

Optimizing light conditions for enhanced strawberry growth in tropical lowlands
M Adrian; Muhammad Yudha Fauzan; Muh Agust Nur Fathoni; Roedhy Poerwanto; Eiichi Inoue; Deden Derajat Matra
Acta Agrobotanica, 22 Nov. 2024, [Reviewed]

ジネンジョ（Dioscorea japonica Thunb.）‘稲武2号’のカリウム吸収特性　　　　　　　　　　　　　　　
Yoshitaka Nakamura; Tetsushi Tanaka; Masahiro Kasuya; Katsutoshi Taki; Toshiya Ootake and Eiichi Inoue, Last, Japanese Society for Horticultural Science (JSHS)
Hort. Res. (Japan), 15 Jul. 2024, [Reviewed]

Effects of Air and CO₂ Application within a Strawberry Plant Canopy on Fruit Yield and Dry Matter Production during Summer and Autumn Culture
Yuya Mochizuki; Ryuta Ninohei; Manato Ohishi; Yukio Yonezu; Tsuyoshi Okayama; Eiichi Inoue, Japanese Society for Horticultural Science
The Horticulture Journal, 15 Jan. 2024, [Reviewed]

Responses of Kecapi (Sandoricum koetjape Merr.) Seedlings Under Different Spectrum and Intensity of Light Emitting Diode
Asyhuriyah Wardah Defitrianida; Deden Derajat Matra; Roedhy Poerwanto; Eiichi Inoue; Dhika Prita Hapsari, IOP Publishing
IOP Conference Series: The 5th International Conference on Bioscience, 31 Dec. 2023, [Reviewed]

Transcriptome Dataset of Strawberry (Fragaria x ananassa Duch.) Leaves using Oxford Nanopore Sequencing under LED Irradiation and Application of Methyl Jasmonate and Methyl Salicylate Hormones Treatment
M Adrian; Roedhy Poerwanto; Eiichi Inoue; Deden Derajat Matra, MDPI (Multidisciplinary Digital Publishing Institute)
Data, 17 Jan. 2023, [Reviewed]

Local CO2 Application within Strawberry Plant Canopy Increased Dry Matter Production and Fruit Yield in Summer and Autumn Culture
Yuya Mochizuki; Sou Murakami; Takuro Kobayashi; Kanjana Worarada; Yukio Yonezu; Hiroki Umeda; Tsuyoshi Okayama; Eiichi Inoue, Last, TAYLOR & FRANCIS INC
International Journal of Fruit Science, 10 Aug. 2022, [Reviewed]

Storability of an Interspecific Hybrid Blueberry HoSp-S65G-13 Suitable for Cluster Harvesting
Yuya Mochizuki; Ai Meguro; Ayu Fukuoka; Chieto Miyashita; Kanjana Worarad and Eiichi Inoue, Last, To extend the shipping period of blueberry fruits, we compared storability of the new interspecific hybrid blueberry HoSp-S65G-13 which is suitable for cluster harvesting with that of existing cultivars. Moreover, we investigated the effects of different harvesting methods (individual and cluster harvesting) on storability and fruit quality., Japanese Society for Horticultural Science (JSHS)
Hort. Res. (Japan), 15 Oct. 2021, [Reviewed]

〔Major achievements〕Transcriptome profiling for pericarp browning during long-term storage of intact lotus,root (Nelumbo nucifera)
Kanjana Worarad; Tomohiro Suzuki; Haruka Norii; Yuya Mochizuki; Takashi Ishii; Keiko Shinohara; Takao Miyamoto; Tsutomu Kuboyama; Eiichi Inoue, Last, Springer Nature
Plant Growth Regulation, 11 Aug. 2021, [Reviewed]

Physiochemical and Morphological Properties of Milled Rice Sold in Shanghai, China -Case of 2015 products-
Toyoko Sato; Youji Nitta; Fumitaka Shiotsu; Naomi Asagi; Eiichi Inoue, 日本作物学会
Japanese Journal of Crop Science, 31 Jan. 2021, [Reviewed]

The Characteristic Nitrogen Absorption of Japanese Yam (Dioscorea japonica) ‘Inabu-2 gou’
Yoshitaka Nakamura; Tetsushi Tanaka; Masahiro Kasuya; Katsutoshi Taki; Eiichi Inoue, Last, Japanese Society for Horticultural Science (JSHS)
Hort. Res. (Japan), 15 Jan. 2021, [Reviewed]

Effects of Temperature Condition on Aerial Tuber and Seed Tuber Sprouting, Plumule and Radicle Elongation of Japanese Yam (Dioscorea japonica Thunb.) ‘Inabu-2 gou’
Yoshitaka Nakamura; Tetsushi Tanaka; Masahiro Kasuya; Katsutoshi Taki and Eiichi Inoue, Last, We investigated the effects of temperature on aerial and seed tuber sprouting and plumule and radicle elongation of Japanese yam (Dioscorea japonica Thunb.) ‘Inabu-2 gou’. According to the results, we concluded that the optimal temperature for early growth of aerial and seed tubers was about 30°C and 25–30°C, respectively., Japanese Society for Horticultural Science (JSHS)
Hort. Res. (Japan), 21 Oct. 2020, [Reviewed]

Flavonoids from The Red Leaf Sheaths of Allium fistulosum ‘Hitachi-benikko’ (Ibaraki’s Specialty Red Welsh Onion Cultivar) and Their Antioxidant Activity
Takayuki Mizuno; Risa Nakane; Takashi Kaidzuka; Ishikawa-Takano Yuko; Fumi Tatsuzawa; Eiichi Inoue and Tsukasa Iwashina, Red welsh onion (Allium fistulosum L.) ‘Hitachi-benikko’, which has red leaf sheaths, was bred by Ibaraki Agricultural Center,as a commercial vegetable in Ibaraki Prefecture. In this study, four anthocyanins including a novel glycoside [cyanidin 3-O-(3″-,O-acetyl-6″-O-malonyl)-glucoside] and five flavonols were isolated and characterized by chemical and spectroscopic methods.,The novel anthocyanin was the main component in the underground part of ‘Hitachi-benikko’. Of these flavonols, four were identified as quercetin and its glycosides, which are known to exhibit marked antioxidant activity. Furthermore, we measured the polyphenol content and antioxidant activity (H-ORAC) of the aerial and underground parts of ‘Hitachi-benikko’ and compared them with those of another general welsh onion cultivar. As the results, the reddish underground parts of ‘Hitachi-benikko’ showed higher polyphenol contents and antioxidant activities than the aerial parts of ‘Hitachi-benikko’ and those of the general,welsh onion cultivar. These results promote the value of red welsh onion cultivar ‘Hitachi-benikko’ as a vegetable., Japanese Society for Horticultural Science (JSHS)
Hort. Res. (Japan), 15 Jul. 2020, [Reviewed]

〔Major achievements〕Genetic evidence that Chinese chestnut cultivars in Japan are derived from two divergent genetic structures that originated in China
Sogo Nishio; Shuan Ruan; Yutaka Sawamura; Shingo Terakami; Norio Takada; Yukie Takeuchi; Toshihiro Saito; Eiichi Inoue, Last, Public Library of Science
PLOS ONE, 01 Jul. 2020, [Reviewed]

〔Major achievements〕Transcriptome analysis of watercore in Pyrus pyrifolia by comparing pairs of susceptible and resistant F1 sibs
Chikako Nishitani; Eiichi Inoue; Toshihiro Saito; Natsumi Ogata; Koichi Kita; Takeru Gonai; Masakazu Kasumi; Ryoji Ishii; Yutaka Sawamura; Norio Takada; Yukino Nakamura; Masaaki Kobayashi; Kentaro Yano; Shingo Terakami; Toshiya Yamamoto, Watercore is a physiological disorder of Japanese pear fruits, in which a watery translucent area appears in the flesh of fruits on the tree. The disorder leads to the loss of commercial value, and resistance to watercore is one of the most important features to be studied in the breeding of Japanese pear. Even in susceptible cultivars such as 'Hosui' (syn. 'Housui'), the degree of watercore development varies markedly from year to year, which makes it difficult to analyze the characteristics of this phenomenon prior to visible watercore development. We used two F1 sib pairs from 'Hosui' x 'Hougetsu' and 'Akiakari' x 'Taihaku' crosses. Each pair contains a susceptible sib and a resistant sib. In these pairs, susceptible sibs developed severe watercore in all the fruits examined in each year, whereas no watercore was found even in overmature fruits of resistant sibs. To search for genes related to watercore, we used a custom pear microarray and studied differential gene expression in susceptible and resistant sibs prior to visible watercore development. We identified 115 differentially expressed genes including sugar metabolism-, hormone-, and cell wall-related genes. Some of these genes were differentially expressed only in immature fruits prior to watercore development. These genes would be useful as molecular markers to predict watercore at early developmental stages or in pear breeding programs., Elsevier
Scientia Horticulturae, 05 Apr. 2020, [Reviewed]

The study on application of AI and image analysis technology to enhancing the efficient agriculture　　　　　　　　　　　　　　　
NOBUOKA Takashi; INOUE Eiichi; YOSHIDA Koshi; YOKOTA Ayaka, 農業農村工学会 水文・水環境研究部会
Applied Hydrology, 27 Mar. 2020

Metabolomic analyses of plasma and liver of mice fed with immature Citrus tumida peel
Atsushi Toyoda; Mizuho Sato; Masaki Muto; Tatsuhiko Goto; Yuji Miyaguchi & Eiichi Inoue, In this study, we investigated the effects of dietary supplementation of Citrus tumida hort. ex Tanaka on food intake, body and fat tissue weights, and metabolic profiles of plasma and liver in mice. Supplementation with 5% (w/w) of peels of immature C. tumida (PIC) for 4 weeks significantly suppressed body weight gain and decreased adipose tissue weight in epididymal, perirenal, and subcutaneous fats. Metabolome analyses showed that 2-hydroxyvaleric acid levels were reduced in the blood plasma of mice fed with PIC. PIC supplementation significantly elevated dipeptide (Thr-Asp, Ser-Glu, and Ala-Ala), glucuronic acid, and S-methylglutathione levels, and significantly reduced betaine aldehyde levels in the liver. In conclusion, PIC supplementation affects the metabolism of fatty acids, pectin, glutathione, and choline, showing potential beneficial effects for metabolic syndrome and obesity. PIC may be developed as a functional food and used in the treatment of these diseases., Japan Society for Bioscience, Biotechnology, and Agrochemistry
Bioscience, Biotechnology, and Biochemistry, 04 Feb. 2020, [Reviewed]

Cacao (Theobroma cacao L.) somatic embryos development under heat stress condition
N Ajija; Syafaruddin and Eiichi Inoue, Last, IOP Publishing
IOP Conference Series: Earth and Environmental Science, 11 Jan. 2020, [Reviewed]

〔Major achievements〕Comparative Transcriptome Analysis of Translucent Flesh Disorder in Mangosteen (Garcinia mangostana L.) Fruits in Response to Different Water Regimes
Deden Derajat Matra; Toshinori Kozaki; Kazuo Ishii; Roedhy Poerwanto; Eiichi Inoue, Last, Public Library of Science
PLOS ONE, 19 Jul. 2019, [Reviewed]

Dietary intake of the citrus flavonoid hesperidin affects stress-resilience and brain kynurenine levels in a subchronic and mild social defeat stress model in mice
Mizuho Sato; Alato Okuno; Kazunori Suzuki; Natsuki Ohsawa; Eiichi Inoue; Yuji Miyaguchi & Atsushi Toyoda, Depressive disorders are partly caused by chronic inflammation through the kynurenine (KYN) pathway. Preventive intervention using anti-inflammatory reagents may be beneficial for alleviating the risk of depression. In this study, we focused on the Japanese local citrus plant, Citrus tumida hort. ex Tanaka (C. tumida; CT), which contains flavonoids such as hesperidin that have anti-inflammatory actions. The dietary intake of 5% immature peels of CT fruits slightly increased stress resilience in a subchronic and mild social defeat (sCSDS) model in mice. Moreover, the dietary intake of 0.1% hesperidin significantly increased stress resilience and suppressed KYN levels in the hippocampus and prefrontal cortex in these mice. In addition, KYN levels in the hippocampus and prefrontal cortex were significantly correlated with the susceptibility to stress. In conclusion, these results suggest that dietary hesperidin increases stress resilience by suppressing the augmentation of KYN signaling under sCSDS., Japan Society for Bioscience, Biotechnology, and Agrochemistry
Bioscience, Biotechnology, and Biochemistry, 23 May 2019, [Reviewed]

ヘスペリジンの給与は慢性社会的敗北ストレスによる脳内キヌレニンの増加を抑制し、社会的ストレスに対する抵抗性を付与する　　　　　　　　　　　　　　　
佐藤 瑞穂; 奥野 海良人; 鈴木 一徳; 大澤 夏樹; 井上 栄一; 宮口 右二; 豊田 淳, (公社)日本栄養・食糧学会
日本栄養・食糧学会大会講演要旨集, Apr. 2019

Dietary Intake of Immature Citrus tumida Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity
Mizuho SATO; Tatsuhiko GOTO; Eiichi INOUE; Yuji MIYAGUCHI; Atsushi TOYODA, Japan Society of Nutrition & Food Science ,and The Vitamin Society of Japan
Journal of Nutritional Science and Vitaminology, 28 Feb. 2019, [Reviewed]

Datasets for genome assembly of six underutilized Indonesian fruits
Matra, D.D.; Ritonga, A.W.; Natawijaya, A.; Poerwanto, R.; Sobir; Siregar, U.J.; Widodo, W.D.; Inoue, E., Last, Elsevier
Data in Brief, Feb. 2019, [Reviewed]

Dataset of the first de novo transcriptome assembly of the arillode of Baccaurea motleyana
Deden Derajat Matra; Arya W Ritonga; Azis Natawijaya; Roedhy Poerwanto; Sobir Sobir; Winarso D Widodo; Eiichi Inoue, Last, Elsevier
Data in Brief, Feb. 2019, [Reviewed]

Dataset from de novo transcriptome assembly of Nephelium lappaceum aril
Deden Derajat Matra; Arya W Ritonga; Azis Natawijaya; Roedhy Poerwanto; Sobir Sobir; Winarso D Widodo; Eiichi Inoue, Last, Elsevier
Data in Brief, Feb. 2019, [Reviewed]

Hybridization of highbush blueberry (Vaccinium corymbosum) in section,Cyanococcus with two Vaccinium section Bracteata species native to subtropical Asia
Chieko Miyashita; Eiichi Inoue; Tetsuya Yamada; Isao Ogiwara, Elsevier
Scientia Horticulturae, 12 Jul. 2018, [Reviewed]

Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
Fitmawati; Roslina Fauziah; Ibna Hayati; Nery Sofiyanti; Eiichi Inoue; Deden Derajat Matra, Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus is therefore as a potential germplasm source in Sumatra. The aim of this study was to analyze and compare the nucleotide sequences of Mangifera in the central region of Sumatra based on gene loci of trnL-F intergenic spacer. The initial step of research was conducted by isolating DNA from leaves sample of Mangifera based on CTAB method. Sequences of gene loci of trnL-F were analyzed with Maximum Parsimony and Neighbour Joining methods through PAUP* Program version 4.0b10. From the results of sequences analysis with Maximum Parsimony method, it was obtained a monophyletic group of Mangifera consisted of two main clades which separated M. kemanga from five other species of Mangifera, i.e. M. foetida, M. odorata, M. laurina and Mangifera sp. Parsimony analysis also revealed that the common ancestor of M. kemanga is the first species appeared in the central region of Sumatra. The result of Neighbour Joining analysis showed M. laurina had the longest genetic distance among examined species and might be assumed as the most primitive species of Mangifera. Mangifera laurina and M. odorata were closely related as well as M. foetida and M. odorata. The results of research also revealed and supported the genus Mangifera as a monophyletic group., Society for Indonesian Biodiversity
Biodiversitas, 01 Jun. 2017, [Reviewed]

未熟フクレミカンの果皮給与がC57BL/6Jオスマウスの体重と代謝産物に与える影響　　　　　　　　　　　　　　　
豊田 淳; 佐藤 瑞穂; 井上 栄一, (公社)日本栄養・食糧学会
日本栄養・食糧学会大会講演要旨集, Apr. 2017

〔Major achievements〕De novo transcriptome assembly of mangosteen (Garcinia mangostana L.) fruit
Deden Derajat Matra; Toshinori Kozaki; Kazuo Ishii; Roedhy Poerwanto; Eiichi Inoue, Garcinia mangostana L. (Mangosteen), of the family Clusiaceae, is one of the economically important tropical fruits in Indonesia. In the present study, we performed de novo transcriptomic analysis of Garcinia mangostana L. through RNA-Seq technology. We obtained the raw data from 12 libraries through Ion Proton System. Clean reads of 191,735,809 were obtained from 307,634,890 raw reads. The raw data obtained in this study can be accessible in DDBJ database with accession number of DRA005014 with bioproject accession number of PRJDB5091. We obtained 268,851 transcripts as well as 155,850 unigenes, having N50 value of 555 and 433 bp, respectively. Transcript/unigene length ranged from 201 to 5916 bp. The unigenes were annotated with two main databases from NCBI and UniProtKB, respectively having annotated-sequences of 73,287 and 73,107, respectively. These transcriptomic data will be beneficial for studying transcriptome of Garcinia mangostana L., Elsevier Inc
Genomics Data, 01 Dec. 2016, [Reviewed]

未熟フクレミカン果皮の給与がマウスの増体に及ぼす影響　　　　　　　　　　　　　　　
佐藤 瑞穂; 白取 知大; 後藤 達彦; 井上 栄一; 豊田 淳, (公社)日本栄養・食糧学会
日本栄養・食糧学会大会講演要旨集, Apr. 2016

〔Major achievements〕Analysis of Allelic Diversity and Genetic Relationships Among Cultivated Mangosteen (Garcinia mangostana L.) in Java, Indonesia Using Microsatellite Markers and Morphological Characters
Deden Derajat Matra; Roedhy Poerwanto; Edi Santosa; Sobir; Hisao Higashio; Hiroyuki Anzai; Eiichi Inoue, The genetic variation and relationships of the mangosteen (Garcinia mangostana L.) were observed across mangosteen populations in Java, Indonesia using newly identified microsatellite loci and morphological characters. In this study, we developed an improved protocol to isolate microsatellite loci, named Selective Repeats from AFLP Sequence, by using a hybridized membrane. Twenty microsatellite loci were evaluated using 78 individuals from five mangosteen populations, and we successfully amplified four closely related Garcinia species, including G. malaccensis, G. hombroniana, G. celebica, and G. porrecta. Eight loci were monomorphic and the others were polymorphic. Sixty-nine alleles were found, with 3.491 per locus on an average. Genetic diversity (HE1) was calculated with an average across loci within population (HES1) as 0.39, an average loci across many populations (HET1) as 0.444, and genetic differentiation (FEST1) as 0.147. Furthermore, based on morphological characters, mangosteen individuals from four populations including Leuwiliang, Wanayasa, Puspahiang, and Kaligesing also had morphologically distinct fruit weight, rind weight, and rind thickness among populations. The study also elucidated the dispersal pattern of mangosteen in Java; the source of the genotype mangosteen in Java population was the Wanayasa population. In addition, we found evidence of tetraploidy in mangosteen. These results have potential applications in future breeding, conservation studies, and genetic assessment of mangosteen and their closely related species., SPRINGER
TROPICAL PLANT BIOLOGY, Mar. 2016, [Reviewed]

Effect of Yacon Tuber (Smallanthus sonchifolius)-derived Fructooligosaccharides on the Intestinal Flora and Immune System of OVA-sensitized BALB/c Mice
Yuji Miyaguchi; Takuya Tomatsuri; Atsushi Toyoda; Eiichi Inoue; Yasuki Ogawa, Yacon (Smallanthus sonchifolius) tuber contains fructooligosaccharides (FOS). FOS are known as prebiotics that beneficially affect the host, i.e., improve the intestinal flora balance and enhance the immunomodulatory potential. In this study, we investigated the effect of a yacon extract rich in FOS (YOS) on the intestinal flora and immune system of BALB/c mice sensitized and challenged with ovalbumin in comparison with an extract with acid-degraded FOS (YAH). During the 4-week test period, the amount of YOS intake was lower than that of YAH; however, a significant increase in body weight was not shown among any of the test groups. Sneezing events were observed in OVA-treated mice. It was found that administration of yacon FOS improved the intestinal flora. Further, it was suggested that administration of yacon-derived FOS could epidemiologically improve the Th1/Th2 cytokine balance through its prebiotic effect on the number of beneficial bacteria in the animal, KARGER
FOOD SCIENCE AND TECHNOLOGY RESEARCH, Mar. 2015, [Reviewed]

Development of an Efficient Selection Method for the Parthenocarpic Trait of Tomato
Tetsushi Tanaka; Tetsuya Oyabu and Eiichi Inoue, Last, To breed varieties with the parthenocarpic trait for laborsaving of tomato cultivation, we investigated an efficient selection,method for the trait. In spring and autumn when self-fertilization occurs, working hours of stigma excision were shorter than,those of emasculation. The decisions of the parthenocarpic trait in each treatment generally corresponded. These findings show,that stigma excision is effective in actual breeding. In winter when self-fertilization is difficult, it is important to control night,temperature to the lowest of 8°C or higher and select individuals with seedless fruits enlarging normally from ones flowering,after the start of night temperature control. Parthenocarpy can be selected efficiently by stigma excision in spring and autumn,,or by the method using low temperature in winter., 一般社団法人 園芸学会
園芸学研究, 26 Dec. 2014, [Reviewed]

〔Major achievements〕Identification of QTLs controlling harvest time and fruit skin color in Japanese pear (Pyrus pyrifolia Nakai)
Toshiya Yamamoto; Shingo Terakami; Norio Takada; Sogo Nishio; Noriyuki Onoue; Chikako Nishitani; Miyuki Kunihisa; Eiichi Inoue; Hiroyoshi Iwata; Takeshi Hayashi; Akihiro Itai; Toshihiro Saito, Using an F-1 population from a cross between Japanese pear (Pyrus pyrifolia Nakai) cultivars 'Akiakari' and 'Taihaku', we performed quantitative trait locus (QTL) analysis of seven fruit traits (harvest time, fruit skin color, flesh firmness, fruit weight, acid content, total soluble solids content, and preharvest fruit drop). The constructed simple sequence repeat-based genetic linkage map of 'Akiakarr consisted of 208 loci and spanned 799 cM; that of 'Taihaku. consisted of 275 loci and spanned 1039 cM. Out of significant QTLs, two QTLs for harvest time, one for fruit skin color, and one for flesh firmness were stably detected in two successive years. The QTLs for harvest time were located at the bottom of linkage group (LG) Tai3 (nearest marker: BGA35) and at the top of LG Tail5 (nearest markers: PPACS2 and MEST050), in good accordance with results of genome-wide association study. The PPACS2 gene, a member of the ACC synthase gene family, may control harvest time, preharvest fruit drop, and fruit storage potential. One major QTL associated with fruit skin color was identified at the top of LG 8. QTLs identified in this study would be useful for marker-assisted selection in Japanese pear breeding programs., JAPANESE SOC BREEDING
BREEDING SCIENCE, Dec. 2014, [Reviewed]

Early Selection Method for Introducing Short-internode-trait in Tomato
Tetsushi Tanaka; Tetsuya Oyabu; Yoshiyuki Asano and Eiichi Inoue, Last, To produce a laborsaving variety with a short-internode-trait in tomato, the early selection method for the short-internode trait was examined using breeding material with parthenocarpy. ‘Rinka 409’ was selected as a promising material with a short internode-trait. The results indicate that it is possible to,efficiently introduce the short-internode-trait into parthenocarpic tomato using the plant height in the early growth period., 一般社団法人 園芸学会
園芸学研究, 27 Dec. 2013, [Reviewed]

S2 Progeny of Japanese Pear (Pyrus pyrifolia Nakai) 'Osa Nijisseiki' Having Self-compatibility and Their Homozygosity Estimated By SSR Analysis
Eiichi Inoue
Hortscience, 2013

Effect of Cooking on the Fructooligosaccharide Content of Yacon Root
Yuji Miyaguchi; Eiichi Inoue; Teruo Tsukihashi, Yacon (Smallanthus sonchifolius), a vegetable which originates from the Andean region in South America, was introduced to ]apan about 20 years ago, Yacon root is known to be a good source of fructooligosaccharides (FOS).,However, the effects of cooking on the FOS content in roots have not been sufficiently investigated. In this study, roots of four species of yacon (Peru-A， Sarada-Otome， Andesu-No-Yuki and Sarada-Okame) were cooked by several methods, such as boiling, microwaving, and steaming, and FOS was extracted from the treated roots and evaluated., 公益社団法人 日本食品科学工学会
Nippon Shokuhin Kagaku Kogaku Kaishi, 15 Apr. 2012, [Reviewed]

Evaluation of Self-fertility among Chestnut Varieties on Shading Treatment of the Flower and Genetic Analysis of the SSR Loci in Self-pollinated Seedlings
Eiichi Inoue; Takashi Homma; Michiyasu Sasaki; Takeru Gonai; and Masakazu Kasumi, Lead, クリの自家不結実性は,他の果樹のようには完全でないと報告されているが,その自家結実性の程度については明らかになっていない。そこで本論文では,花粉遮断試験とSSR遺伝子座の遺伝解析によって,品種間における自家結実性の変異を明らかにした。花粉遮断試験の結果,調査した51品種・系統の約47%にあたる24種類において結実が確認された。そのうちの5品種とその自家結実実生13個体について,SSR遺伝子座の遺伝を確認したところ'伊吹','大峰'および'豊多摩早生'由来の8個体が自殖実生であると推察された。特に,'豊多摩早生'に関しては着毬率(62.5%)と結果率(33.3%)も他の品種・系統と比較して極めて高く,調査した6個体すべてが自殖実生であることが確認された。一方,'笠原早生','丹沢'および'大峰'に由来する5個体は,他家受粉に由来すると考えられた。以上の結果より,クリにおいては品種によっては自家結実が可能であるが,それはごく低頻度であり,基本的には自家不結実であると結論した。しかし,今回用いた品種・系統のうち,豊多摩早生'だけは自家結実性と判断した。, 一般社団法人 園芸学会
Horticultural Research (Japan), 15 Apr. 2012, [Reviewed]

Improvement of Pretreatment Drip Device Fertigation Technique Using Methane Fermentation Digestion Liquid and the Effect in Cucumber
Mioko Yoshino; Tatsuo Sato; Ani Widiastuti; Tasuku Kato; Eiichi Inoue; Masahide Iwata; Kenji Fukushi; Koji Iwashita; Fumio Watanabe, 一般社団法人 園芸学会
Horticultural Research (Japan), 28 Feb. 2012, [Reviewed]

Genetic Mapping and QTL Analysis in Interspecific Chestnut Population
Eiichi Inoue
Hortscience, 2012

Effect of Cooking on the Fructooligosaccharide Content of Yacon Root
Yuji Miyaguchi; Eiichi Inoue; Teruo Tsukihashi, Yacon (Smallanthus sonchifolius), a vegetable which originates from the Andean region in South America, was introduced to Japan about 20 years ago. Yacon root is known to be a good source of fructooligosaccharides (FOS). However, the effects of cooking on the FOS content in roots have not been sufficiently investigated. In this study, roots of four species of yacon (Peru-A, Sarada-Otome, Andesu-No-Yuki and Sarada-Okame) were cooked by several methods, such as boiling, microwaving, and steaming. and FOS was extracted from the treated roots and evaluated. Furthermore, the effects of peeling and grating on FOS content in the root were investigated. The following results were obtained: 1) Yacon FOS decreased markedly by storage at 4 degrees C for 1 month, 2) FOS decreased by heating at 100 degrees C after peeling and grating, 3) FOS in species, except for Sarada-Otome, was little influenced by treatment with 1% acetic acid for 1. hour, 4) FOS dramatically decreased with deep-frying and was little influenced by heating by methods such as boiling, microwaving, baking and steaming, and 5) Yacon extracts from Sarada-Otome containing 4% FOS had decreased emulsifying activity and increased emulsion stability. These findings suggest that high concentrations of FOS from yacon root could be obtained by using suitable cooking conditions., JAPAN SOC FOOD SCIENCE TECHNOLOGY
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 2012, [Reviewed]

Komatsuna Pot Culture Tests for Fertilizer and Environmental Effect for Digestive,Liquid of Methane Fermentation
KATO Tasuku; INOUE Eiichi; SATO Tatsuo; FUKUSHI Kenji; and IWASHITA Koji, In application of digestive liquid of methane fermentation as fertilizer, fertilizer and environmental effects when digestive liquid contained much organic nitrogen are considered through Komatsuna pot culture. Three types pot culture tests were conducted; much irrigation water application, fertilizer application before seeding and fertilizer effect of different organic nitrogen contents of digestive liquids in seven fermentation facilities.
農業農村工学会資源循環研究部会論文集, Nov. 2011, [Reviewed]

Evaluation of polyphenol content and other properties of 16 cultivars/strains of Yacon
Makiko Takenaka; Kazuko Nanayama; Eiichi Inoue, Last, This study aimed to evaluate the polyphenol content and other properties of a number of cultivars/strains of yacon (Smallanthus sonchifolius), and select nutritionally superior cultivars/strains that would contribute to improve human health. The tuberous roots and tubers of 3 cultivars and 13 strains of yacon were examined. Among the 3 cultivars, 'Saradaotome' and 'Saradaokame' showed superior polyphenol content in the tuberous roots. Among the 16 cultivars/strains. 'Peru-1', 'Peru-3' and 'Peru-4' showed superior polyphenol content, especially in the tuber, and 'CIP205029' showed the highest saccharide level. A positive correlation was observed between polyphenol contents in the tuberous root and tuber of the same yacon cultivar/strain. (Received Jun. 8, 2011 : Accepted Jul. 14, 2011), JAPAN SOC FOOD SCIENCE TECHNOLOGY
Nippon Shokuhin Kagaku Kogaku Kaishi, 01 Nov. 2011, [Reviewed]

クリ果実における糖とデンプン含有量の品種間差並びに，加工グリの不良品発生要因　　　　　　　　　　　　　　　
本間貴司; 原弘道; 阮樹安; 陳喜忠; 王志運; 井上栄一; 月橋輝男
全球視角下的当代農業問題研究, 2011, [Reviewed]

Development of Simple Sequence Repeat Markers in Chinese Chestnut and Their Characterization in Diverse Chestnut Cultivars
Eiichi Inoue; Lin Ning; Hiromichi Hara; Shuan Ruan; Hiroyuki Anzai, To develop and characterize valid simple sequence repeat (SSR) markers in chestnut (Castanea spp.), we used a selective hybridization method to perform SSR sequence enrichment in the genomic library of chinese chestnut (Castanea mollissima). From 47 sequences of the enriched library, we designed 24 primer pairs for SSR polymerase chain reaction (PCR). SSR PCR was performed for 23 chinese chestnut cultivars. Among the 24 primers, 22 primers amplified their respective SSR loci; 21 primer pairs yielded polymorphic fragments across the cultivars. Among these 21 primer pairs, 17 primer pairs amplified single SSR loci with polymorphisms. Multilocus amplification patterns were observed in the other four primers. For the corresponding 17 SSR loci, the number of alleles per locus was two to 13, and the average number of alleles was 7.19. The observed heterozygosity (number of genotypes of the heterozygote/total number of genotypes scored at that locus) ranged from 0.00 to 0.87 (average = 0.68), and the expected heterozygosity ranged from 0.00 to 0.87 (average = 0.68). One of the SSR loci-ICMA014-was a unique locus, because the primer pair for this locus did not amplify any fragment in any of the cultivars, except in Qianchi, which was used to construct the SSR-enriched genomic library. Cross-species amplifications using the 17 primer pairs were also successful in the other species of genus Castanea. A dendrogram depicting the relationships among the genotypes on the basis of genetic distances was generated using the unweighted pair group method with arithmetic mean cluster analysis. The genotypes were clearly separated into three large groups, which reflected the three cultivated species, namely, C. mollissima, C. erenata, and C. saliva., AMER SOC HORTICULTURAL SCIENCE
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, Nov. 2009, [Reviewed]

Development of SSR Markers in Chinese Chestnuts and their Characterization in Diverse Chestnuts Cultivars
Eiichi Inoue; Lin Ning; Hiromichi Hara; Shuan Ruan; Hiroyuki Anzai, Lead, The American Society for Horticultural Science
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, Nov. 2009, [Reviewed]

Scanning Electron Microscopy of Strage Reserves Mobiization in Japanese Chestnut Fruit (Castanea crenata Siebold & Zucc.) during Strage Period
Takashi Homma; Eiichi Inoue; Toshiaki; Matsuda; Hiromichi Hara, Mobilization of storage reserves in Japanese chestnut fruit was observed by scanning electron microscope during the storage period. We stored chestnuts at −20, 2, 8 and 20°C to examine the effects of temperature on mobilization of reserves during 12-week period after harvest. At harvest (shedding stage), the cotyledonary parenchyma cells were filled with amyloplast (plastid containing a amount of substantial starch granules) and it's amount was gradually increased from the center region (adaxial region) of the cotyledon to the outer region (abaxial region) of the cotyledon. As the storage period progressed, the number of amyloplast per cell was declined in fruit stored at 2, 8 and 20°C and the rate of decline differed at each storage temperatures. Namely, higher temperatures facilitated starch decomposition. This is thought to occur because the storage temperature affects the respiration rate and starch decomposition products provide substrates for respiration. In nuts stored at −20°C, mobilization of reserves was not recognized during the storage period. The rate of starch decomposition also differed among regions of the cotyledon. At the outer region of cotyledon, the rate of decomposition was high and the inner region was low. Corresponding to the high rate of starch decomposition, an abundance of cytoplasm was observed between and surface of the amyloplast. It is suggested that the amount of cytoplasm was related to starch decomposition activities.
, 一般社団法人 園芸学会
Horticultural Research (Japan), 25 Oct. 2009, [Reviewed]

Characteristics of Interspecific Hybrids between Japanese Chestnut (Castanea crenata Sieb et Zucc.) and Seguin's Chestnut (C. seguinii Dode)
Masakazu Kasumi; Takeru Gonai; Eiichi Inoue; Toru Manabe; Fumio Sakuma, ニホングリ（Castanea crenata Sieb. et Zucc.）とモーパングリ（C. seguinii Dode）の種間交配を行い、得られた種間雑種の特性を調査した。種間交配ではニホングリの品種交配に比べて種子形成率がやや低下するものの、発芽率はほぼ同等であった。交配実生は、RAPDによるDNA分析、形態特性から交配ミスによるニホングリ間の交配組合せと判明した個体を除くと、三度栗型実生とわい性型実生の二つに分類でき、その出現頻度はほぼ1：1に分離した。三度栗型実生は樹高がニホングリ間の交配実生と同じかやや低く、三度栗と同じように新梢に雌花を断続的に着生するものであった。わい性型実生は樹高がニホングリ間の交配実生の半分程度以下で、枝の伸長がやや少なく、結実樹齢に達するのが遅く、果実はモーパングリよりやや大きい程度（2〜3g）できわめて小さかった。このため、モーパングリを用いてわい性（3m以下）のニホングリ経済品種を育成するには、わい性を維持しつつ、‘石鎚’程度の果重（25g以上）、早期結実性（5年生までに）が必須となり、今後ニホングリとの数回の戻し交配が必要と考えられる。
identifier:780783
identifier:ZZ00017577, 茨城県農業総合センター生物工学研究所
Bull. Ibaraki Plant Biotech. Inst, Mar. 2009, [Reviewed]

CLONING OF A CDNA ENCODING THE SUCROSE: SUCROSE 1-FRUCTOSYLTRANSFERASE (1-SST) FROM YACON AND ITS EXPRESSION IN TRANSGENIC RICE
W. Pan; Y. Sunayama; Y. Nagata; M. Taniguchi; M. Takano; E. Inoue; H. Tamagake; H. Anzai, Yacon is a perennial plant grown in the Andes, which was introduced into Japan for its high accumulation of inulin-type fructan in tuberous roots in 1980s. We isolated a 1-SST gene (Ye 1-SST) from cDNA library derived from yacon mature tuberous root. The coding region of the gene is 1920bp long, and its deduced protein consists of 640 amino acids with a predicted molecular mass of 72kDa. The deduced amino acid sequence of Yc 1-SST shares 87% identity with 1-SST (EC 2.4.1.99) from Jerusalem artichoke (Helianthus tuberosus L). A functional analysis for Yc 1-SST was performed by transforming into rice (Oryza saliva L. cv.Nipponbare) and compared with rice transformed with 1-SST gene of Jerusalem artichoke (Ja 1-SST) under the regulation of the cauliflower mosaic virus (CaMV) 35S promoter Transgenic rice overexpressing Yc 1-SST produced fructan such as 1-kestose (GF(2)), 1-nystose (GF(3)) and 1-fructosylnystose (GF(4)), which showed the same activity as Ja 1-SST., DIAGNOSIS PRESS LTD
Biotechnology & Biotechnological Equipment, 01 Jan. 2009, [Reviewed]

Simple Sequence Repeat (SSR) Analysis of 7 Chestnut Cultivars in the Korean Peninsula Using SSR Markers of Japanese Chestnut
Eiichi Inoue; Lin Ning; Toshiya Yamamoto; Shuan Ruan; Yumi Matsuki; Hiroyuki Anzai; Hiromichi Hara, Lead, Using 7 pairs of simple sequence repeat (SSR) primers developed for Japanese chestnut, we identified the SSR genotypes of 7 chestnut cultivars that originated from the Korean peninsula and compared them with those of 17 Chinese chestnut cultivars, 32 Japanese chestnut cultivars, and an interspecific hybrid obtained by crossing the above species. The 7 SSR loci of all cultivars were successfully genotyped, except KT006a for which no amplification products were obtained from 3 cultivars of Chinese chestnut. Among the 36 alleles observed in the 17 Chinese cultivars, 26 were shown to be species-specific alleles, while 37 of the 47 alleles observed in the 32 Japanese cultivars were shown to be specific to Japanese chestnut. The SSR genotypes of the Korean cultivars were also identified and comprised 2-9 alleles per locus (average: 5.14 alleles). By comparing the results to the species-specific alleles, we considered that 'Myeonsan 9', 'Myeonsan 13', 'Myeonsan 60', 'Inhungwanglyul', and 'Han 6' are Japanese chestnut cultivars; 'Hamjong 3 ho' is a Chinese chestnut cultivar; and 'Ooshiro' is an interspecific hybrid. A synonymous relationship or a bud mutation was estimated between 'Han 6' and 'Tanzawa', and between 'Ooshiro' and 'Riheiguri'.
, 一般社団法人 園芸学会
Horticultural Research (JAPAN), 25 Oct. 2008, [Reviewed]

Changes in Fruit Quality Factors in Japanese Chestnut (Castanea crenata Siebold & Zucc.) During Long-term Storage
Takashi Homma; Eiichi Inoue; Toshiaki Matsuda and Hiromichi Hara, Long-term storage of Japanese chestnut (Castanea crenata Siebold & Zucc.) fruit using a polyethylene bag was carried out between October 2004 to September 2005. In order to create different O₂/CO₂ compositions in the bags, we used 1, 2, or 5 layers of polyethylene bags. The concentrations of soluble sugars and starch and the microstructure of cotyledonary parenchyma cells were investigated as fruit quality factors. The rate of sound fruits was decreased for all treatments at 2 months of storage. When 5 layers of polyethylene bag were used, all fruits lost their commercial quality at 10 months of storage. At 3 months of storage, there were large differences in gas composition among the treatments. The higher number of polyethylene films facilitated a decrease in the O₂ content and increase in the CO₂ content. Scanning electron microscopic observation of cotyledonary parenchyma cells demonstrated that the degree of starch breakdown in each treatment was also different at 3 months of storage. Starch breakdown was extensive when 1 or 2 layers of polyethylene bag were used but was decreased when 5 layers of polyethylene bag were used. These morphological differences indicate that gas composition in the bags affects fruit respiration and reserves mobilization. The amyloplast density and starch concentration in sound fruits were relatively high at 11 months of storage when 1 and 2 layers of polyethylene bag were used. At this stage, although the sound fruits rate was low (approx. 50%), a high amount of storage reserves was retained in these fruits. These findings suggest that the amount of storage reserves in chestnut fruit at harvest affects fruit storability; in other words, well-matured fruits have a good potential for long-term storage.
, 一般社団法人 園芸学会
Horticultural Research(Japan), 25 Oct. 2008, [Reviewed]

Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai)
Eiichi Inoue; Yumi Matsuki; Hiroyuki Anzai; Kate Evans, Fourteen microsatellite markers were developed from an enriched genomic library of Japanese pear (Pyrus pyrifolia) by selective hybridization. They were characterized using 17 Japanese pear cultivars. The expected heterozygosity and observed heterozygosity ranged from 0.21 to 0.74 and from 0 to 0.88, respectively. Two to 11 alleles were detected per locus, with IPPN09 and IPPN15 judged to amplify multiple loci. IPPN17 was the most informative locus with the lowest probability of identity (0.19). These primers exhibited a high cross-species transferability between species and genera., BLACKWELL PUBLISHING
MOLECULAR ECOLOGY NOTES, May 2007, [Reviewed]

Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai)
Eiichi Inoue; Yumi Matsuki; Hiroyuki Anzai; Kate Evans, Lead, Wiley
Molecular Ecology Resources (Molecular Ecology Notes), 29 Nov. 2006, [Reviewed]

精神科デイケアの園芸療法による主観的ウェルビーイングと家族との交流の変化－農学との連携による心の活力ネットワークの広がり－
山川百合子; 高橋弘美; 佐々木俊子; 今井忠則; 小松崎将一; 井上栄一; 池田正則; 山口彩子; 中川昌子; 登坂ユカ; 新井雅信, 本研究の目的は，園芸療法により精神障害者自身あるいは家族に対しどのような効果をもたらすかについて検討することである。精神科デイケアで農学の専門家とデイケアスタッフである医療者が連携して園芸療法プログラムを策定した。, 茨城県立医療大学, 日本バランスオブライフ研究会
均衡生活学, Oct. 2006, [Reviewed]

精神科デイケアにおける園芸療法の心理的効果と検討〜地域リハビリテーションと農学の連携〜
Yuriko Yamakawa; Shoichi Komatsuzaki; Eiichi Inoue; Masanori Ikeda; Saeko Yamaguchi; Masako Nakayama; Hiromi Takahashi; Toshiko Sasaki; Yuka Tosaka; Masanobu Arai, The purpose of this study was to investigate the psychological effects of horticultural,therapy (HT) on psychiatric outpatients at the Ibaraki Prefectural University of Health,Sciences Hospital., 茨城県立病院医学雑誌編集委員会
茨城県立病院医学雑誌, Sep. 2006, [Reviewed]

Overcoming hybrid lethality in a cross between Japanese pear and apple using gamma irradiation and confirmation of hybrid status using flow cytometry and SSR markers
T Gonai; T Manabe; E Inoue; M Hayashi; T Yamamoto; T Hayashi; F Sakuma; M Kasumi, Although there have been some attempts to obtain intergeneric hybrids between Japanese pear (Pyrus pyrifolia Nakai) and apple (Malus x domestica Borkh.) mature hybrids have not been generated due to hybrid lethality. Shoots from immature hybrid embryos were gamma irradiated and cultured at normal temperature conditions to obtain viable intergencric plants. Gamma irradiation doses of 50 and 100 Gy cultured at 25 degrees C killed all shoots but there was one survivor at 150 Gy and 25 T. Hybrid status of the shoot was confirmed by using morphology, flow cytometry and SSR markers. The intergeneric plant had similar leaf serrations to apple and similar petioles to Japanese pear. Flow cytometric analysis revealed that the intergeneric shoot had a DNA content intermediate between the parents. SSR analysis showed one allele from each parent for all 16 SSR loci investigated. These data strongly suggest that the obtained shoot is a true hybrid between Japanese pear and apple. (c) 2006 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
SCIENTIA HORTICULTURAE, Jun. 2006, [Reviewed]

Identification of RAPD marker linked to fruit skin color in Japanese pear (Pyrus pyrifolia Nakai)
E Inoue; M Kasumi; F Sakuma; H Anzai; K Amano; H Hara, In Japanese pear, fruit skin color is a very important trait for growers because the russet skin protects the fruit against external stress caused by disease, insects, bad weather, and shipping. This report describes the development of a randomly amplified polymorphic DNA (RAPD) marker linked to major genes controlling the fruit skin color in Japanese pear, Two F, progenies from the cross of 'Kousui' x 'Kinchaku' and 'Niitaka' x 'Chikusui' segregated by fruit skin color were used for bulked segregant analysis. Four kinds of bulked DNA were constructed and used for the polymerase chain reaction in RAPD analysis. After 200 random primers were screened against four bulks, a band named OPH-19(425) was selected in cooperation with green bulks. The recombination rate between OPH-19(425) and the green skin phenotype was 7.3%. The RAPD marker (OPH-19425) could select green fruit with probability as high as approximately 92%. The marker was apparently useful for the selection of green-skinned individuals in a breeding program. This is the first report on developing a DNA marker closely linked to the fruit phenotype in Japanese pear. (c) 2005 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
SCIENTIA HORTICULTURAE, Feb. 2006, [Reviewed]

Production and characterization of recombinant human lactoferrin in transgenic Javanica rice
D Rachmawati; T Mori; T Hosaka; F Takaiwa; E Inoue; H Anzai, The objective of the present study was to develop a functional crop that could contribute to the maintenance and improvement of human health by the introduction of the human lactoferrin (hLF) gene. Lactoferrin is an 80-kDa iron-binding glycoprotein that has been considered to play many biological roles, including the regulation of iron absorption, protection against microbial and virus infection, stimulation of the immune system and cellular growth promotion. We introduced two different constructs containing either the native signal peptide from human lactoferrin (pIG211) or the signal peptide from rice glutelin (pIG200) fused to mature human lactoferrin into Javanica rice cv. Rojolele by using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the maize ubiquitin-1 promoter was detected in all the tissues of the transgenic plants. We found that the transgenic rice plants IG200R produced a considerable amount of recombinant hLF (rhLF) in seeds, accounting for approximately 15% of the total soluble protein (TSP). RhLF was purified from mature seeds by cation-exchange chromatography. Amino acid sequencing confirmed that the N-terminal sequences of rhLF for both constructs were identical with those of native LF from human milk. Rice rhLF showed a slightly smaller molecular weight than the native hLF. Immunofluorescence microscopy revealed that rhLF was located in the intracellular and intercellular regions of endosperm cells. Protein extracts from transgenic rice seeds exhibited an antibacterial activity against Bacillus subtilis ATCC6633. The use of signal peptides and a constitutive ubiquitin-1 promoter for successful production of transgenic Javanica rice expressing a high level of rhLF was examined., JAPANESE SOC BREEDING
BREEDING SCIENCE, Jun. 2005, [Reviewed]

Interspecific Hybridization of Wild Gladiolus Species Using a Dendrogram Based on RAPD Analysis
Yasumasa Takatsu; K. Suzuki; Tetsuya Yamada; Eiichi Inoue; T. Gonai; M. Nogi and Masakazu Kasumi, We have obtained information on relationships among wild gladiolus species through RAPD analysis (Takatsu et al., 2001b). Interspecific hybridization has been carried out based on a dendrogram and F(1) seeds have been derived from seven reciprocal crosses. However matured seed was not obtained from the crosses using Gladiolus orchidiflorus as a female parent. We have made a segregation population (F(2)) derived from interspecific hybridization between G tristis and G gracilis for selection of a RAPD marker linked to a floral scent trait of the wild species G gracilis. Floral scent of each plant was evaluated by sensory evaluation in the F(2) plants and it was shown that a segregation ratio of scented plant and non-scented plant fit a 1:3 (chi(2-)value=0.09) ratio., International Society for Horticultural Science
Acta Horticulturae (ISHS), 31 May 2005, [Reviewed]

Maternal haploidization on Japanese pear through intergeneric hybridization with apple
Eiichi Inoue; Fumio Sakuma; Masakazu Kasumi; Hiromichi Hara; Teruo Tsukihashi, Lead, We report on two methodologies for developing maternal haploids in Japanese pear. In the first method, we tried to induce haploidization through an inter-generic hybridization with irradiated pollen of apple. Fourteen seedlings germinated from 155 F-1 seeds that were sown. Five seedlings at 120Gy total dosage (20Gy/h dose rate) and a seedling at 80Gy (10Gy/h) survived and grew vigorously. Their morphology and DNA profiling indicated that they were similar to Japanese pear. Secondly, we tried to obtain apomicts through an inter-generic cross between a specific cultivar of Japanese pear 'Gold Nijisseiki' and apple with the intact pollen. Only one seedling survived from the 53 inter-generic seedlings that germinated. Its characteristics were also similar to the morphology of Japanese pear. Though the individual did not inherit any RAPD markers from apple, it has two SSR alleles inherited from apple., International Society for Horticultural Science
Acta Horticulturae (ISHS), Dec. 2004, [Reviewed]

Agrobacterium-mediated transformation of Javanica rice cv. Rojolele
Diah Rachmawati; Takehiko Hosaka; Eiichi Inoue; Hiroyuki Anzai, The Agrobacterium-mediated transformation system was extended to a famous Javanica rice variety, Rojolele, that is cultivated in Indonesia now. Efficient callus induction from immature and mature seeds of Rojolele did not succeed by any previous method for any rice cultivar. In this study, the callus from mature seeds of Rojolele exhibited a compact and nodular appearance on C medium after the carbon source and medium pH was modified. Scutellum-derived calli from mature seeds were co-cultivated with Agrobacterium tumefaciens strains EHA101 or LBA4404 that carried plasmid pAFT14, which contained the genes for beta-glucuronidase (gus) and hygromycin resistance (hpt). Finally, the transformation efficiency of Rojolele variety using A. tumefaciens strain EHA101 (pAFT14) was improved to about 23%, similar to that of the Japonica rice variety Nipponbare. The seed fertility of transgenic Rojolele was more than 90%. The copy number of the transgene varied from one to three copies in the To transgenic lines. Both the gus and the hpt genes were inherited and expressed in the progeny., Japan Society for Bioscience, Biotechnology, and Agrochemistry
Biosci. Biotechnol. Biochem., Jun. 2004, [Reviewed]

Origin and RAPD Analysis of Japanese Chestnut (Castanea crenata Sieb. et Zucc.) Cultivated in China
Eiichi Inoue; Hiromichi Hara; Shuan Ruan; Masami Sano; Teruo Tsukihashi, Lead, Japanese chestnut is a traditional nut in Japan. Though almost all the produce from Japanese chestnut trees grown in China are shipped to Japan for processing, their characterization has not been reported. In this paper, we report the origin and discrimination of varieties in China using randomly amplified polymorphic DNA (RAPD) analysis. All varieties examined in this report could be discriminated by 162 kinds of RAPD markers raised from 8 primers. Four varieties introduced to China from Japan seemed to be seedlings of the same varieties being cultivated in Japan. One of the varieties introduced from Japan, 'Moriwase' had a low similarity to 'Moriwase' in Japan. This finding suggests that 'Moriwase' in China was misinterpreted during the process by which had been introduced from Japan, or propagated via seedling. Five varieties bred in China were classified in the same group as the old varieties in Japan.
, 園芸学会
Horticultural Research (Japan), Dec. 2003, [Reviewed]

Effect of high-temperature on suppression of the lethality exhibited in the intergeneric hybrid between Japanese pear (Pyrus pyrifolia Nakai) and apple (Malus x domestica Borkh.)
Eiichi Inoue; Fumio Sakuma; Masakazu Kasumi; Hiromichi Hara; Teruo Tsukihashi, Lead, Hybrid lethality is a difficult obstacle that must be overcome in order to produce mature intergeneric hybrids between Japanese pear and apple. High-temperature has been shown to be effective in suppression of hybrid lethality in rice, tobacco and cotton. If a similar response to temperature is observed in the hybrid between Japanese pear and apple, suppression of the lethality should lead to vigorous growth of the hybrid. In this experiment, we observed whether the expression of the lethality was suppressed at high-temperature or not. Clear differences in vegetative growth were observed among the shoots derived from the immature embryo at different temperature conditions. The shoots at 34°C grew vigorously without exhibiting the lethal symptoms. Similar phenomena was also observed in F1 seedlings of the cross. More than 80% of the intergeneric seedlings at high-temperature conditions (34/30°C, day/night and 34°C) grew without lethal symptoms within 18 days after sowing. Although the high-temperature suppressed the lethal symptoms, the seedlings died within 5 months from the resulting stress. In this study, we clarified that the hybrid lethality between Japanese pear and apple was suppressed at high-temperature in a similar manner to those of hybrids from wide crosses in other plant species. This phenomenon gives the possibility for studying and overcoming lethality. © 2003 Elsevier Science B.V. All rights reserved., Elsevier
Scientia Horticulturae, 29 Sep. 2003, [Reviewed]

Effect of high-temperature on suppression of the lethality exhibited in the intergeneric hybrid between Japanese pear (Pyrus pyrifolia Nakai) and apple (Malus x domestica Borkh.)
Eiichi Inoue; Fumio Sakuma; Masakazu Kasumi; Hiromichi Hara; Teruo Tsukihashi, Lead, Elsevier
Scientia Horticulturae, 29 Sep. 2003, [Reviewed]

Production of apomict-like seedlings through intergeneric cross between Japanese pear and apple by means of pollen irradiation
Eiichi Inoue; Fumio Sakuma; Masakazu Kasumi; Hiromichi Hara; Teruo Tsukihashi, Lead, Apomixis may provide a new possibility for pear genetics, breeding and propagation. In this experiment we tried to produce apomictic seedlings in Japanese pear through an intergeneric cross between Japanese pear and apple by means of pollen irradiation. The false hybrid can be efficiently selected in this intergeneric crossing. Because the true intergeneric hybrid potent genetic lethal system, only the false hybrid with the possibility of apomixis should survive in the crossing. Japanese pear (Pyrus pyrifolia Nakai) 'Kousui' and apple (Malus pumila Mill.) 'Fuji' were used for the intergeneric cross. The apple pollen was irradiated 6 by gamma ray from Co-60 and pollinated on the Japanese pear. A total of fourteen intergeneric seedlings at 10Gy/h (50 and 80Gy) and 20Gy/h (60 and 120Gy) developed from 155 seeds, and an individual at 80Gy (10Gy/h) and five individuals at 120Gy (20Gy/h) survived without exhibiting lethal symptoms. Plant type and morphological characters of five apomict-like seedlings at 120Gy were similar to those of Japanese pear. Their RAPD markers were only inherited from Japanese pear and their DNA contents estimated by FCM were also similar to those of Japanese pear. From the above results, we conclude that five apomict-like seedlings may be true apomicts in Japanese pear., International Society for Horticultural Science
Acta Horticulturae (ISHS), Oct. 2002, [Reviewed]

Plant regeneration through leaf culture of Yacon
Masaru Niwa,Takeomi Arai,Kazushi Fujita,Wataru Marubashi,Eiichi Inoue,Teruo Tsukihashi, Corresponding, Yacon (Smallanthus sonchifolius), a member of Asteraceae, is an ethnic food of Andeans. Interest in this crop is increasing because of its high fructo-oligosaccharide eontent in the tuberous roots. Due to the low productivity of viable seeds and virus infections through vegetative propagation, effective methods of in vitro propagation of yacon is being sought. To accomplish this, leaf pieces of yacon were cultured on the gelrite-solidified MS media, supplemented with various combinations of auxins and cytokinins. The most effective means of inducing calli was achieved on a MS medium that was supplemented with 0.1 mg・liter^<-1> 2, 4-D and 0.1 mg・liter^<-1> BAP. Shoot regeneration occurred when the calli were subcultured on the HaR medium (Paterson and Everett, 1985), supplemented with 1.0 mg・liter^<-1> BAP and 0.1 mg・liter^<-1> GA_3. By sub culturing those calli at a monthly interval, the shoot regeneration continued up to the 10th subculture. The regenerated shoots rooted ; the plantlets were well-acclimatized and transplanted successfully to the field. AFLP analysis conducted on the extracted DNA from the regenerants revealed 12 out of the 13 possessed different amplified fragments from those of the mother plants, indicating that the somaclonal mutation took place during the course of callusing and plant regeneration., 一般社団法人 園芸学会
J. Japan Soc. Hort. Sci., 15 Jul. 2002, [Reviewed]

Evaluation of germplasm collection in wild Gladiolus species of Southern africa
Yasumasa Takatsu; Toru Manabe; Masakazu Kasumi; Tetsuya Yamada; Ryuji Aoki; Eiichi Inoue; Yoich Morinaka; Wataru Marubashi; Mikio Hayashi, 21種のグラジオラス野生種について特性調査および育種素材としての評価を行ったところ, 草丈, 葉数, 葉·花の形態, 小花数および開花期等には種によって大きな違いがみられた. また草丈が10cm程度で鉢物に利用可能なもの, 現在の栽培種にはみられない青色の花被片を有するものなど, 育種素材として有望な野生種が見出された. 香りを有する種は全体の52.3%を占め, 香りのタイプもチョウジ様, スミレ様などさまざまであることが示された. さらにこれらの野生種について到花日数, 小花の開花期間, 稔実日数および1さや当たりの種子数を調査し育種上重要な情報を得ることができた. フローサイトメトリーによる解析の結果, 野生種においては細胞あたりのDNA含量が多様で, 種によってイネの0.9∼3.5倍のゲノムサイズを有するものと推定された. 本法による倍数性の判定は困難であるが, 種の組合せによっては交雑後代の雑種性の検定に利用可能であることが示唆された., 日本育種学会
Breeding Research, Jun. 2002, [Reviewed]

Interspecific hybridization of wild gladiolus species of southern Africa, based on random amplified polymorphic DNA markers
Yasumasa Takatsu; Masaru Miyamoto; Eiichi Inoue; Tetsuya Yamada; Masakazu Kasumi; Toru Manabe; Mikio Hayashi; Fumio Sakuma; Wataru Marubashi; Masaru Niwa, We obtained information on the genetic relationship in wild Gladiolus species through randomly amplified polymorphic DNA (RAPD) analysis. Out of the 140 tested primers, 32 amplified a total of 133 RAPD bands in 33 Gladiolus species. The genetic distance was calculated from the data of these RAPDs, and a dendrogram was generated. Interspecific crosses were carried out in seven combinations within or between clusters, and F1 seedlings were obtained from most combinations. The RAPD analysis showed that these F1 seedlings were real hybrids. The results suggest that RAPD markers are useful for detecting genetic relationships in Gladiolus species, and for interspecific crosses in breeding programs. (C) 2001 Elsevier Science B.V. All rights reserved., Elsevier
Scientia Horticulturae, 14 Dec. 2001, [Reviewed]

Tempareture effects on interspecific hybridization between Gladiolus x grandiflora and G. tristis
Yasumasa Takatsu; Masakazu Kasumi; Toru Manabe; Mikio Hayashi; Eiichi Inoue; Wataru Marubashi; Masaru Niwa, Interspecific hybridization between a modern cultivar of Gladiolus xgrandiflora hort. (2n = 60) and the wild species G. tristis L. (2n = 30) was made to introduce characteristics of the wild species into the cultivated one. Gladiolus xgrandiflora is a summer-flowering species, and G. tristis flowers in winter. The effect of storage temperature on pollen viability was tested, as long-term storage of pollen was necessary to facilitate crossing these two species. Pollen of G. tristis could he stored st -20 degreesC for approximate to1 year, and was more practical than storage at -80 degreesC. Air temperature affected pollen tube growth, fertility, and fruit set in the cross between G. xgrandiflora and G. tristis, and low temperatures (15 to 20 degreesC) were best. The morphological data and flow cytometric analysis showed that the F(1) plants were hybrids between G. xgrandiflora and G. tristis., American Society for Horticultural Science
HortScience, Apr. 2001, [Reviewed]

Characterization and overcoming of temperature dependent lethality exhibited by the hybrid embryos from the cross Nicotiana suaveolens x N. sylvestris
Eiichi Inoue; Wataru Marubashi; Masaru Niwa, Lead, Hybrid lethality is one of the expressions of cross-incompatibility, limiting the scope of wide hybridization. The immature embryos of the cross Nicotiana suaveolens x N. sylvestris did not grow and could not be rescued by conventional ovule culture. We observed that culture of the ovules at high temperatures (30, 32, 34 degreesC) enabled to suppress this obstacle temporarily. Although many plantlets were obtained through ovule culture at high temperatures, the plants did not grow to maturity. On the other hand, ovule culture with a high concentration of cytokinin was effective in overcoming the obstacle. In ovule culture on 1/2 MS + BAP (10 mg/l), many hybrid plantlets of the cross N. suaveolens x N. sylvestris developed and formed vigorous multiple shoots. The hybrid plants obtained from multiple shoots grew vigorously and matured in a greenhouse. Because the characteristics of the obstacle were similar to those of hybrid lethality exhibited by the seedlings of the cross between N. suaveolus and N. tabacum, we suggested that the obstacle was due to hybrid lethality that developed just after fertilization. Furthermore, we were able to obtain many mature hybrid plantlets in the cross N. suaveolens x N. sylvestris by ovule culture on a medium containing cytokinin. This is the first report on the production of mature interspecific hybrids that did not develop any lethal symptoms in this cross combination., Japanese Society of Breeding
Breeding Science, 01 Dec. 2000, [Reviewed]

Development of DNA marker linked to fruit skin color in Japanese pear (Pyrus pyrifolia Nakai)
Eiichi Inoue; Katsuki Amano; Masakazu Kasumi; Fumio Sakuma; Hiromichi Hara; Teruo Tsukihashi, National Institute of Agrobiological Resources
MAFF Int. Work. Gen. Res. Ser., 28 Nov. 2000

Intergeneric crossing between Japanese pear (Pyrus pyrifolia Nakai) and Apple (Malus pumila Mill.)
Fumio Sakuma; Eiichi Inoue; Masakazu Kasumi, ニホンナシにリンゴ果実の持つ芳香性や高糖度，貯蔵性など優良な形質を導入しようとして，ニホンナシとリンゴの属間で正逆交雑を試みた。その結果，ニホンナシを種子親とした場合，4613花交配して21.7%の結果率であった。一方リンゴを種子親にした場合は，2232花交配して9.3%の結果率であった。特に，ニホンナシ'幸水'およびリンゴ'ふじ'を種子親にした交配組み合わせで結果率が高かった。しかし，ニホンナシを種子親にした場合22.1%，リンゴを種子親にした場合11.1%の無種子果が発生した。特に，ニホンナシ'幸水'を種子親，リンゴ'王林'を種子親または花粉親とした場合無種子果の発生が多かった。属間交配果の稔実種子数は，ニホンナシを種子親とした場合は1果当たり2.0個で品種間交配の2.7個より少なかった。また，リンゴを種子親にした場合は1果当たり1.2個で品種間交配の2.3個より減少した。しかし，種子重に大きな差はみられなかった。属間交雑種子の発芽率は，ニホンナシを種子親を種子親にした場合1013粒播種して61.6%，リンゴを種子親にした場合117粒播種して29.1％であった。それに対して品種間交雑種子の発芽率は，ニホンナシでは59.5%，リンゴでは81.3%でリンゴを種子親にした属間交配で発芽率がかなり劣った。実生は播種16日後から5ヶ月の間に雑種致死のためすべて枯死した。交配70日後の胚を培養することによりシュートが得られた。胚培養由来シュートは34℃の高温条件下で雑種致死を抑制できたが，やはり枯死し雑種致死は回避できなかった。また，胚培養により得られ，30日毎に継代培養しているシュートはWPM培地でピロガロールおよびIBA処理により発根が促進できたが，順化後枯死した。, 茨城県農業総合センター生物工学研究所
Bull. Ibaraki Plant Biotech. Inst, Mar. 2000

Improvement of the method for overcoming hybrid lethality between Nicotiana suaveolens and N. tabacum by culture of F1 seeds in liquid media containing cytokinins
Eiichi Inoue; Wataru Marubashi; Masaru Niwa, Lead, Hybrid lethality system is one of the most serious barriers in wide hybridization. Hybrid plants between N. suaveolens and N. tabacum exhibit lethality and die at the cotyledonary stage. Intact seedling culture on solidified media containing cytokinins is a simple method for overcoming the lethality in this cross-combination. In order to improve the method, we cultured F1 seeds in liquid media containing cytokinins. Two types of cytokinins, purine-type (kinetin, 6-benzylaminopurine (BAP) and t-zeatin) and urea-type (N-phenyl-N'-(1, 2, 3-thidiazol-5-yl) urea (TDZ), N-(2-chloro-4-pyrizyl)-N'-phenylurea (CPPU)), were used at various concentrations (0-10 mg/l). In the liquid media containing 0.01-10 mg/l cytokinin, shoot-like structures were formed at the hypocotyl and the primary root of the dying seedlings. The shoot-like structures did not exhibit lethality and grew up to vigorous shoots without developing any lethal symptoms within a month. Vigorous multiple shoots developed from the shoot-like structures in the liquid media containing more than 0.2 mg/l cytokinins. The regenerated shoots were transferred to the medium containing 2 mg/l indole-3-acetic acid (IAA) to accelerate rooting. The hybrid seedlings displayed a vigorous growth without developing any lethal symptoms up to maturity in a greenhouse. Forty chromosomes were counted in root tip cells of the seedlings and morphological characters of the seedlings were intermediate between those of parental species. In this experiment, we developed a simplified method using liquid media with urea-type cytokinins and obtained more hybrid seedlings in a shorter period of time., Japanese Society of Breeding
Breeding Science, 01 Sep. 1997, [Reviewed]

Improvement of the method for overcoming the hybrid lethality between Nicotiana suaveolens and N-tabacum by culture of F-1 seeds in liquid media containing cytokinins
E Inoue; W Marubashi; M Niwa, Hybrid lethality system is one of the most serious barriers in wide hybridization. Hybrid plants between N. suaueolens and N. tabacum exhibit lethality and die at the cotyledonary stage, Intact seedling culture on solidified media containing cytokinins is a simple method for overcoming the lethality in this cross-combination. In order to improve the method, we cultured F-1 seeds in liquid media containing cytokinins. Two types of cytokinins, purine-type (kinetin, 6-benzylaminopurine (BAP) and t-zeatin) and urea-type (N-phenyl-N'-(1, 2, 3-thidiazol-5-yl) urea (TDZ), N-(2-chloro-4-pyrizyl)-N'-phenylurea (CPPU)), were used at various concentrations (0-10 mg/l). In the liquid media containing 0.01-10 mg/l cytokinin, shoot-like structures were formed at the hypocotyl and the primary root of the dying seedlings. The shoot-like structures did not exhibit lethality and grew up to vigorous shoots without developing any lethal symptoms within a month, Vigorous multiple shoots developed from the shoot-like structures in the liquid media containing more than 0.2 mg/l cytokinins. The regenerated shoots were transferred to the medium containing 2 mg/l indole-3-acetic acid (IAA) to accelerate rooting. The hybrid seedlings displayed a vigorous growth without developing any lethal symptoms up to maturity in a greenhouse, Forty chromosomes were counted in root tip cells of the seedlings and morphological characters of the seedlings were intermediate between those of parental species, In this experiment, we developed a simplified method using liquid media with urea-type cytokinins and obtained more hybrid seedlings in a shorter period of time., JAPANESE SOC BREEDING
BREEDING SCIENCE, Sep. 1997, [Reviewed]

Genomic factors controlling the lethality exhibited in the hybrid between Nicotiana suaveolens Lehm and N-tabacum L
E Inoue; W Marubashi; M Niwa, Interspecific hybrid plants between Nicotiana suaveolens and N. tabacum exhibit lethal symptoms at the seedling stage and cannot grow to maturity. In this investigation, an attempt was made to clarify the genomic factors responsible for this lethality. N. suaveolens was crossed to N. sylvestris (genomic constitution: SS) and N. tomentosiformis (TT), these latter two species being the progenitors of N. tabacum (SSTT). From the cross N. suaveolens x N. tomentosiformis, many seedlings were obtained through ovule culture, and these subsequently grew to maturity without exhibiting any lethality. In the reciprocal crossing between N. sauvelons and N. sylvestris, only a few hybrid seedlings were obtained through ovlue culture and all died after unfolding their cotyledons when cultured at 28 degrees C. This lethality could be avoided by culturing the ovules at 36 degrees C. These features of hybrid lethality resembled those observed in the interspecific hybrid between N. suaveolens and N. tabacum. These findings suggest that the S genome in N. tabacum is responsible for the lethality exhibited in the hybrid between N. suaveolens and N. tabacum., SPRINGER VERLAG
THEORETICAL AND APPLIED GENETICS, Aug. 1996, [Reviewed]

Simple method for overcoming the lethality observed in the hybrid between Nicotiana suaveolens and N. tabacum
Eiichi Inoue; Wataru Marubashi; Masaru Niwa, Lead, Hybrid plants between N. suaveolens and N. tabacum exhibit lethality under standard culture conditions and die at the cotyledonary stage. For overcoming this type of hybrid lethality, the effect of cytokinin was tested. The intact hybrid seedlings were cultured on media containing cytokinin (0-10mg/l kinetin or 6-benzylaminopurine (BAP)). Adventitious buds were regenerated from the base of the hypocotyl or the primary root of the dying seedlings. The regenerated shoots were transferred to the medium containing 2mg/l 3-indoleacetic acid (IAA) to accelerate rooting. The hybrid seedlings maintained a vigorous growth without developing any lethal symptoms up to maturity in a greenhouse. Forty chromosomes were counted in their somatic cells and their morphological characters were intermediate between those of parental species. Intact seedling culture with the addition of cytokinin was found to be a very simple method for overcoming lethality in this cross-combination., Japanese Society of Breeding
Breeding Science, 01 Sep. 1994, [Reviewed]

Characteristics of intergeneric seedling between Japanese pear and apple with gamma irradiated pollen
YU B.; 郷内武; 望月佑哉; 井上栄一
園芸学研究 別冊, 2021

〔Major achievements〕高い機能性を保持した粉末・ペースト作製技術
井上栄一; 石井貴
研究成果パンフレット：収穫後品質の向上と機能性を活かした加工品の展開による国産レンコンのブランド力強化プロジェクト, Mar. 2020, [Reviewed]
Corresponding

〔Major achievements〕品種識別ＤＮＡマーカーの種レンコン生産における活用技術
堀井学; 八城和敏; 久保山勉; 篠原啓子; 井上栄一
研究成果パンフレット：収穫後品質の向上と機能性を活かした加工品の展開による国産レンコンのブランド力強化プロジェクト, Mar. 2020, [Reviewed]
Last

〔Major achievements〕最適な温度条件、通気条件、被覆材に基づく生レンコンの輸送技術
石井貴; 井上栄一
研究成果パンフレット：収穫後品質の向上と機能性を活かした加工品の展開による国産レンコンのブランド力強化プロジェクト, Mar. 2020, [Reviewed]
Corresponding

伝統野菜レンコン（蓮根）のブランド力強化－高品質な国産レンコンの需要拡大と輸出に向けて
井上栄一; 久保山勉
Chemistry and chemical industry, 01 Dec. 2019, [Invited]

HRM法によるレンコンの簡易品種識別法の開発
仮屋亜由美; 堀井学; 白澤健太; 篠原啓子; 澤田英司; 八城和敏; 樋口洋平; 石川祐聖; 井上栄一; 久保山勉
育種学研究, 16 Mar. 2019

ddRAD‐seq解析より得られたSNPに基づくレンコン品種識別法の開発
小林希美; 白澤健太; 堀井学; 篠原啓子; 澤田英司; 八城和敏; 樋口洋平; 石川祐聖; HAQUE E; 井上栄一; 久保山勉
育種学研究, 25 Mar. 2018

ニホンナシ果実の‘みつ症’発生機構に関する研究(第7報)倍数性の異なるニホンナシ‘豊水’の果実におけるみつ症の発症と成熟・糖代謝に関わる遺伝子の関係
八塚拓; 野口尚美; 尾形夏海; 喜多晃一; 郷内武; 石井亮二; 西谷千佳子; 山本俊哉; 東尾久雄; 井上栄一
園芸学研究 別冊, 2016

ニホンナシ果実の‘みつ症’発生機構に関する研究(第6報)‘豊水”染色体倍加個体の果実における高いみつ症感受性
井上栄一; 八塚拓; 野口尚美; 尾形夏海; 西谷千佳子; 喜多晃一; 郷内武; 石井亮二; 山本俊哉; 東尾久雄
園芸学研究 別冊, 2016

Genetic Diversity in Chestnut Germplasm Assessed by Nuclear and Chloroplast Simple Sequence Repeat Markers
Eiichi Inoue; Miki Kurabayashi; Lin Ning; Takashi Homma; Hiromichi Hara
HORTSCIENCE, Aug. 2010

Annual Differences of Sugar Production in Sweet Sorghum (Sorghum bicolor (L.) Moench) Stem
KAMIYAMA Akinori; NARISAWA Kazuhiko; KURUSU Yasurou; OHTA Hiroyuki; CHOHNAN Shigeru; MIYAGUCHI Yuji; TOYODA Atsushi; KATO Tasuku; KOBAYASHI Hisashi; KOMATSUZAKI Masakazu; SATO Tatsuo; NITTA Youji; YOSHIDA Koushi; HOMMA Takashi; UMEHARA Ryouhei; KOBAYASI Ryo; MATSUDA Toshiaki; NAKAMURA Satoshi; GOTO Yusuke; INOUE Eiichi
Tohoku Journal of Crop Science, 2009

Effect of Weather Condition on Sugar Yield in Sweet Sorghum (Sorghum bicolor (L.) Moench) Stem
KAMIYAMA Akinori; NARISAWA Kazuhiko; KURUSU Yasurou; OHTA Hiroyuki; CHOHNAN Shigeru; MIYAGUCHI Yuji; TOYODA Atsushi; KATO Tasuku; KOBAYASHI Hisashi; KOMATSUZAKI Masakazu; SATO Tatsuo; NITTA Youji; YOSHIDA Koushi; HOMMA Takashi; UMEHARA Ryouhei; KOBAYASI Ryo; MATSUDA Toshiaki; NAKAMURA Satoshi; GOTO Yusuke; INOUE Eiichi
Tohoku Journal of Crop Science, 2009

45 バイオアルコール生産を目指したスィートソルガムの栽培制御研究(栽培,日本作物学会第225回講演会)
新田洋司; 神山朗範; 松田智明; 中村聡; 後藤雄佐; 井上栄一; 成澤才彦; 久留主泰朗; 太田寛行; 長南茂; 豊田淳; 加藤亮; 小林久; 小松崎将一; 佐藤達雄
日本作物學會紀事, 27 Mar. 2008

Genetic comparison among chestnuts and the related species in Fagaceae by cpSSR markers
Eiichi Inoue; Lin Ning; Hiroyuki Anzai; Hiromichi Hara
HORTSCIENCE, Jul. 2007

図説 園芸学 第2版　　　　　　　　　　　　　　　
荻原勲; 井上栄一, Joint work
朝倉書店, 01 Oct. 2020
9784254410402

麹菌による発酵がカンキツ果皮粉末のポリメトシキフラボノイド含有量に及ぼす影響　　　　　　　　　　　　　　　
阿武佑弥; 藤井智教; 大西賢一; 柳橋 諒; 長谷川守文; 豊田 淳; 望月佑哉; 井上栄一
園芸学会 令和7年度春季大会, 21 Mar. 2025, 園芸学会
20250320, 20250321

ブドウ‘シャインマスカット’へのケイ素施用が生育および果実品質に及ぼす影響　　　　　　　　　　　　　　　
諏訪光太; 早川智恵; 池田裕樹; 井上栄一; 山根健治
園芸学会 令和7年度春季大会, 21 Mar. 2025, 園芸学会
20250320, 20250321

洗浄工程がサツマイモ塊根に及ぼす衝撃　　　　　　　　　　　　　　　
荒木田尚広; 渡邊健; 熊谷亨; 井上栄一
園芸学会 令和7年度春季大会, 20 Mar. 2025, 園芸学会
20250320, 20250321

近年の茨城大学果樹研究の成果について　　　　　　　　　　　　　　　
井上栄一
令和７年度茨城県果樹研究会情報交換会, 17 Mar. 2025, 茨城県果樹研究会, [Invited]
20250317

麹菌の資化によるカンキツ果皮中のポリメトシキフラボノイド含有量の増加　　　　　　　　　　　　　　　
阿武佑弥; 藤井智教; 大西賢一; 柳橋 諒; 長谷川守文; 豊田 淳; 望月佑哉; 井上栄一
第22回日本機能性食品医用学会総会, 07 Dec. 2024, 日本機能性食品医用学会
20241207, 20241208

エキシマUVランプを用いた異なる貯蔵前殺菌処理がブルーベリー果実の貯蔵性に及ぼす影響　　　　　　　　　　　　　　　
望月佑哉; 北條大地; 高野友二郎; 芹澤和泉; 井上栄一
園芸学会 令和6年度秋季大会, 04 Nov. 2024, 園芸学会
20241103, 20241104

クリ易渋皮剥皮性品種の堅果の取り扱いが品質に及ぼす影響　　　　　　　　　　　　　　　
井上栄一; 布施達也; 江花拓磨; 山口貴史; 望月佑哉
園芸学会 令和6年度秋季大会, 03 Nov. 2024, 園芸学会
20241103, 20241104

サツマイモのセカンドキュアリングにおける癒傷組織の発達過程　　　　　　　　　　　　　　　
荒木田尚広; 渡邊健; 望月佑哉; 井上栄一
園芸学会 令和6年度春季大会, 24 Mar. 2024, 園芸学会
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ブドウ‘シャインマスカット’の短梢栽培における葉の発達と果実の成熟に及ぼす摘心の影響　　　　　　　　　　　　　　　
諏訪光太; 池田裕樹; 井上栄一; 山根健治
園芸学会 令和6年度春季大会, 24 Mar. 2024
20240323, 20240324

Effects of Air and CO2 Application within Strawberry Plant Canopy on Dry Matter Production and Fruit Yield during Summer and Autumn Culture　　　　　　　　　　　　　　　
Yuya Mochizuki; Ryuta Ninohei; Manato Ohishi; Yukio Yonezu; Tsuyoshi Okayama; Eiichi Inoue
The 4th Asian Horticultural Congress (AHC2023), 29 Aug. 2023, Japanese Society for Horticultural Science (JSHS)
20230828, 20230831

Identification of candidate gene controlling cork formation on apple pericarp by using Malus domestica ‘Goldfarm’ as a bud mutation variety of ‘Fuji’　　　　　　　　　　　　　　　
Eiichi Inoue; Kanjana Worarad; Yuya Mochizuki
The 4th Asian Horticultural Congress (AHC2023), 29 Aug. 2023, Japanese Society for Horticultural Science (JSHS)
20230828, 20230831

Differences Artificial Light Spectrum using Light Emitting Diode on Secondary Metabolite Profiles in Kasturi Mango Seedlings from Tembilahan, Riau, Indonesia　　　　　　　　　　　　　　　
Pandi Ariansah; JL. Meranti; Eiichi Inoue; Roedhy Poerwanto; Deden Derajat Matra
The 4th Asian Horticultural Congress (AHC2023), 29 Aug. 2023, Japanese Society for Horticultural Science (JSHS)
20230828, 20230831

レンコン粉末の麹化によるヌシフェリン産生機構の解明　　　　　　　　　　　　　　　
柳橋諒・藤井智教・大西賢一・長谷川守文・豊田 淳・望月佑哉・井上栄一
園芸学会 令和5年度春季大会, 20 Mar. 2023, 園芸学会
20230319, 20230320

野菜や果実に含まれる健康機能性成分を麹菌で増強する！　　　　　　　　　　　　　　　
井上栄一・柳橋諒・長谷川守文・望月佑哉・豊田淳
東和新生会ビジネス交流会, 09 Nov. 2022, 東和新生会
20221109, 20221109

麹菌による園芸作物の健康機能性成分の増強　　　　　　　　　　　　　　　
井上栄一
新技術説明会, 15 Sep. 2022, 科学技術振興機構、茨城大学、群馬大学、埼玉大学
20220915, 20220915

Characteristics of root development in high-yielding strawberry ‘Benihoppe’ grown in hydroponics under cold environment　　　　　　　　　　　　　　　
Mochizuki; Y.; A. Hosokubo and E. Inoue
園芸学会 令和4年度秋季大会, 08 Sep. 2022, 園芸学会
20220907, 20220913

Observation of watercore in Japanese pear fruit by neutron imaging and angle neutron scattering　　　　　　　　　　　　　　　
Kajiyama; K.; T. Shinohara; E. Inoue and T. Gonai
園芸学会 令和4年度秋季大会, 08 Sep. 2022, 園芸学会
20220907, 20220913

イチゴ夏秋どり栽培における植物群落内へのCO2局所施用が乾物生産および果実収量に及ぼす影響　　　　　　　　　　　　　　　
望月佑哉・村上 颯・小林拓朗・米津幸雄・井上栄一
園芸学会 令和3年度秋季大会, 11 Sep. 2021, 園芸学会
20210908, 20210913

麹化によるレンコン粉末の機能性成分の改変　　　　　　　　　　　　　　　
柳橋諒・藤井智教・大西賢一・長谷川守文・豊田淳・井上栄一
園芸学会 令和3年度秋季大会, 11 Sep. 2021, 園芸学会
20210908, 20210913

Characteristics of intergeneric seedling between Japanese pear and apple with gamma irradiated pollen　　　　　　　　　　　　　　　
Yu; B.; T. Gonai; Y. Mochizuki; E. Inoue
園芸学会 令和3年度秋季大会, 11 Sep. 2021, 園芸学会
20210908, 20210913

アセチルシステインの施用法の違いが四季成り性イチゴ ‘すずあかね’ の乾物生産および個葉光合成速度に及ぼす影響　　　　　　　　　　　　　　　
Meguro; A.; C. Miyashita; Y. Kurokawa; T. Akao; E. Inoue and Y. Mochizuki
園芸学会 令和3年度春季大会, 30 Mar. 2021, 園芸学会
20210329, 20210330

Effect of Low Temperature Storage and Cooking Method on Composition of Polyphenol Compounds in Japanese Chestnut　　　　　　　　　　　　　　　
Suzuki; Y.; T. Fujii; S. Nishio; S. Terakami; M. Hasegawa; Y. Mochizuki and E. Inoue
園芸学会 令和2年度春季大会, 22 Mar. 2020, 園芸学会
20200321, 20200322

Storability of cluster harvesting blueberries　　　　　　　　　　　　　　　
Meguro; A.; C. Miyashita; Y. Kurokawa; T. Akao; E. Inoue and Y. Mochizuki
園芸学会 令和2年度春季大会, 22 Mar. 2020, 園芸学会
20200321, 20200322

Transcriptome analysis of pericarp in lotus root (Nerumbo nucifera) after harvest　　　　　　　　　　　　　　　
Norii; H.; W. Kanjana; K. Shinohara; T. Ishii; T. Miyamoto; Y. Mochizuki and E. Inoue
園芸学会 令和2年度春季大会, 21 Mar. 2020, 園芸学会
20200321, 20200322

ジネンジョ（Dioscorea japonica）‘稲武2号’のカリウム吸収特性　　　　　　　　　　　　　　　
中村嘉孝; 田中哲司; 糟谷真宏; 瀧勝俊; 大竹敏也; 井上栄一
園芸学会 令和2年度春季大会, 21 Mar. 2020, 園芸学会
20200321, 20200322

Water soaking method for long term storage of Lotus roots　　　　　　　　　　　　　　　
Inoue; E.; K. Imai; T. Ishii; K. Shinohara; T. Miyamoto; K. Worarad; H. Norii; Y. Mochizuki and T. Kuboyama
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

収穫後におけるレンコンの表皮の微細構造が外観品質に及ぼす影響　　　　　　　　　　　　　　　
規井榛香; 篠原啓子; 石井貴; 宮本貴夫; Worarad, Kanjana; 望月佑哉; 井上栄一
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

Effect of modified atmosphere (MA) film on the long storage period of Lotus roots,(Nelumbo nucifera Gaertn)　　　　　　　　　　　　　　　
Watanabe; M.; E. Inoue; T. Yamada; T. Miyamoto; and T. Ishii
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

クリにおけるポリフェノール含有量の品種間差と相関分析　　　　　　　　　　　　　　　
鈴木結花; 高橋是成; 望月佑哉; 井上栄一
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

Effect of modified atmosphere packaging on storability of two cultivars and two inter specific hybrids in blueberries　　　　　　　　　　　　　　　
Mochizuki; Y.; A. Meguro; C. Miyashita; Y. Kurokawa; T. Akao and E. Inoue
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

Gene expression profiling of candidate genes for pericarp browning in Lotus Root (Nelumbo nucifera) during long-term storage　　　　　　　　　　　　　　　
Worarad; K.; K. Imai; H. Norii; T. Ishii; K. Shinohara; T. Miyamoto; Y. Mochizuki and E. Inoue
2019 Autumn Annual Meeting of JSHS, 16 Sep. 2019, The Japanese Society for Horticultural Science
20190915, 20190917

アセチルシステインの施肥が‘すずあかね’の乾物生産に及ぼす影響　　　　　　　　　　　　　　　
小林拓朗; 米津幸雄; 岡山毅; 井上栄一; 望月佑哉
園芸学会 令和元年度秋季大会, 16 Sep. 2019, 園芸学会
20190915, 20190917

ジネンジョ（Dioscorea japonica）の栽培におけるパイプ内への施肥の影響　　　　　　　　　　　　　　　
田中哲司; 瀧勝俊; 糟谷真宏; 井上栄一
園芸学会 令和元年度秋季大会, 15 Sep. 2019, 園芸学会
20190915, 20190917

RNA-Seq analysis of the transcriptome and genes expression profile during the browning of Lotus Root (Nelumbo nucifera)　　　　　　　　　　　　　　　
Kanjana Worarad; Haruka Norii; Yuya Muchizuki; Takashi Ishii; Keiko Shinohara; Takao Miyamoto; Eiichi Inoue
International Joint Conference on JSAM and SASJ, and 13th CIGR VI Technical Symposium joining FWFNWG and FSWG Workshops, 06 Sep. 2019, JSAM, SASJ, CIGR, FWFNWG and FSWG
20190903, 20190906

CI-Dextran mixの包接によるフラボノイドの可溶化に関する研究　　　　　　　　　　　　　　　
井上栄一; 中島聡美; 藤井智教; 日野志朗; 堀端大路
日本食品科学工学会 第66回大会, 31 Aug. 2019, 日本食品科学工学会
20190829, 20190831

茨城県産フクレミカン（Citrus tumida hort. ex Tanaka）果実におけるフラボノイド含量　　　　　　　　　　　　　　　
藤井智教; 長谷川守文; 豊田淳; 井上栄一
第73回日本栄養・食糧学会大会（静岡）, 18 May 2019, 日本栄養・食糧学会
20190517, 20190519

Elucidation of the effective anti-browning solution for long term storage of Lotus root (Nelumbo nucifera)　　　　　　　　　　　　　　　
Worarad; K.; H. Norii; K. Imai; T. Ishii; Y. Mochizuki; K. Shinohara; T. Miyamoto and E. Inoue
2019 Spring Annual Meeting of JSHS, 24 Mar. 2019, The Japanese Society for Horticultural Science
20190323, 20190324

クリにおける低温貯蔵および調理方法がポリフェノール含有量に及ぼす影響　　　　　　　　　　　　　　　
鈴木結花; 藤井智教; 高橋是成; 望月佑哉; 井上栄一
園芸学会平成31年度春季大会, 24 Mar. 2019, 園芸学会
20190323, 20190324

オープンイノベーションによる食品の保存流通の革新～プラットフォーム傘下のコンソーシアム概要紹介　　　　　　　　　　　　　　　
井上栄一
食品の革新的保存・流通技術研究開発プラットフォーム公開シンポジウム, 12 Oct. 2018, 食品の革新的保存・流通技術研究開発プラットフォーム
20181012, 20181012

Evaluation of skin color and polyphenol content of lotus roots during short-term storage　　　　　　　　　　　　　　　
Norii; H.; K. Shinohara; T. Ishii; T. Miyamoto; Y. Mochizuki and E. Inoue
園芸学会平成30年度秋季大会, 23 Sep. 2018, 園芸学会
20180922, 20180924

De novo transcriptome analysis revealing putative genes involved in parthenocarpy of highbush blueberry (Vaccinium corymbosum L.)　　　　　　　　　　　　　　　
Worarad; K.; C. Miyashita; Y. Mochizuki and E. Inoue
2018 Autumn Annual Meeting of JSHS, 23 Sep. 2018, The Japanese Society for Horticultural Science
20180922, 20180924

QTL mapping of sensitivity to watercore using Japanese pear hybrid lines which show various symptoms of watercore　　　　　　　　　　　　　　　
Ogata; N.; K. Kita; T. Yamamoto; C. Nishitani; F. Hosaka; E. Inoue and H. Ichige
園芸学会平成30年度春季大会, 24 Mar. 2018, 園芸学会
20180324, 20180325

Variation of polyphenol contents and antioxidant activities among the parts in lotus root　　　　　　　　　　　　　　　
Norii,H.; M. Tomura; K. Shinohara; M. Horii; T. Fujii; R. Ooji; Y. Shouji and E. Inoue
園芸学会平成29年度秋季大会, 03 Sep. 2017, 園芸学会
20170902, 20170904

Gamma ray sensitivity of one-year-old wood in Japanese pear ‘Housui’　　　　　　　　　　　　　　　
Inoue; E.
園芸学会平成29年度秋季大会, 03 Sep. 2017, 園芸学会
20170902, 20170904

ブランド力の強化と保護を礎とした国際戦略～レンコンを事例として～　　　　　　　　　　　　　　　
井上栄一
日本食品工学会第18 回（2017 年度）年次大会, 08 Aug. 2017, 日本食品工学会
20170808, 20170809

国産レンコンのブランド力強化プロジェクトについて　　　　　　　　　　　　　　　
井上栄一; 久保山勉
LS-BT講演会, 07 Feb. 2017, 産業技術総合研究所
20170206, 20170207

国産レンコンのブランド力強化プロジェクト　　　　　　　　　　　　　　　
井上栄一; 久保山勉
LS-BT講演会, 06 Feb. 2017, 産業技術総合研究所
20170206, 20170207

収穫後品質の向上と機能性を活かした加工品の展開による国産レンコンのブランド力強化プロジェクト　　　　　　　　　　　　　　　
井上栄一
食品の革新的保存・流通 技術研究開発プラットホーム 公開シンポジウム, 11 Nov. 2016, 食品の革新的保存・流通 技術研究開発プラットホーム, [Invited]
20161111, 20161111

The fruit russeting in a bud mutated apple variety ‘Gold farm’ originated from ‘Fuji’　　　　　　　　　　　　　　　
Inoue; E.; K. Kohno; M. Hayashi and K. Korenari
園芸学会平成28年度秋季大会, 11 Sep. 2016, 園芸学会
20160910, 20160912

茨城県在来のフクレミカン(Citrus tumida Hort.ex Tanaka)における果実形質の個体間差　　　　　　　　　　　　　　　
藤井智教; 井上栄一
園芸学会平成28年度秋季大会, 11 Sep. 2016, 園芸学会
20160910, 20160912

Study on watercore development in Japanese pear: 6．High sensitivity for watercore in fruit of tetraploid Japanese pear ‘Hosui'　　　　　　　　　　　　　　　
井上栄一; 八塚拓; 野口尚美; 尾形夏海; 西谷千佳子; 喜多晃一; 郷内武; 石井亮二; 山本俊哉; 東尾久雄
園芸学会平成28年度春季大会, 26 Mar. 2016, 園芸学会
20160326, 20160328

Study on watercore development in Japanese pear: 7．Relationship between watercore symptom and genes involved in fruit maturity and sugar metabolism in Japanese pear‘Hosui’with different polyploidy　　　　　　　　　　　　　　　
八塚拓; 野口尚美; 尾形夏海; 喜多晃一; 郷内武; 石井亮二; 西谷千佳子; 山本俊哉; 東尾久雄; 井上栄一
園芸学会平成28年度春季大会, 26 Mar. 2016, 園芸学会
20160326, 20160328

Microarray analysis of gene expression in Japanese pear appearing watercore　　　　　　　　　　　　　　　
園芸学会平成28年度春季大会, 26 Mar. 2016, 園芸学会
20160326, 20160328

シイタケにおけるSSRマーカー開発と品種識別について　　　　　　　　　　　　　　　
井上栄一
日本きのこ学会 2015 年度ワークショップ, 11 Mar. 2016, 日本きのこ学会, [Invited]
20160311, 20160311

フクレミカンの果実生長にともなうポリフェノール含有量と抗酸化活性の変動　　　　　　　　　　　　　　　
井上栄一; 堀江良; 勝部千秋; 東尾久雄
園芸学会平成27年度秋季大会, 27 Sep. 2015, 園芸学会
20150926, 20150928

シイタケにおけるSSR マーカー開発と品種識別　　　　　　　　　　　　　　　
井上栄一; マトラ ドラジャト デデン; 田中徳夫; 清水豊
日本きのこ学会第19回大会, 05 Sep. 2015, 日本きのこ学会
20150904, 20150906

Variation of tree physiology in F1 segregating population of Japanese pear　　　　　　　　　　　　　　　
刘金莎; 東尾久雄; 井上栄一
園芸学会平成27年度春季大会, 29 Mar. 2015, 園芸学会
20150328, 20150329

ブルーベリーおよび日本在来野生種を含めたスノキ属植物８種類間の交雑親和性の評価　　　　　　　　　　　　　　　
宮下千枝子; 井上栄一; 山田哲也; 荻原勲
園芸学会平成27年度春季大会, 29 Mar. 2015, 園芸学会
20150328, 20150329

改良根箱を用いた台木用カボチャの耐湿性評価　　　　　　　　　　　　　　　
石橋雅之; 東尾久雄; 井上栄一
園芸学会平成27年度春季大会, 29 Mar. 2015, 園芸学会
20150328, 20150329

Determination of Nuclear DNA Content on Mangosteen (Garcinia mangostana L.) by Flow Cytometry　　　　　　　　　　　　　　　
Matra; DD; Inoue; E; Higashio H
29th International Horticultural Congress 2014, 22 Aug. 2014, International Society for Horticultural Science (ISHS)
20140817, 20140822

Development of Simple Sequence Repeats Marker in Cultivated Mangosteen (Garcinia mangostana L.) for Their Genetic Diversity Analysis　　　　　　　　　　　　　　　
Matra; DD; Inoue; E; Higashio H
29th International Horticultural Congress 2014, 22 Aug. 2014, International Society for Horticultural Science (ISHS)
20140817, 20140822

QTL for Fruit Skin Traits in Japanese pear　　　　　　　　　　　　　　　
Inoue; E; Nishii; M; Yamamoto; T; Higashio; H
29th International Horticultural Congress 2014, 21 Aug. 2014, International Society for Horticultural Science (ISHS)
20140817, 20140822

ブルーベリーのSSR マーカーを用いたスノキ属22 種の分類　　　　　　　　　　　　　　　
宮下千枝子; 井上栄一; 櫛川聡; 山田哲也; 荻原勲
園芸学会平成26年度春季大会, 30 Mar. 2014, 園芸学会
20140329, 20140330

ニホンナシ果実への拮抗陽イオン施用が可食部への放射性セシウム移行に及ぼす影響　　　　　　　　　　　　　　　
飛田良憲; 高橋是成; 東尾久雄; 井上栄一
園芸学会平成26年度春季大会, 29 Mar. 2014, 園芸学会
20140329, 20140330

果皮色形質に連鎖するDNAマーカーSTS-OPH19によるニホンナシの選抜と選抜有効性の検証　　　　　　　　　　　　　　　
池上秀利; 渡邉辰彦; 石坂晃; 桒原実; 松本和紀; 牛島孝策; 平島敬太; 井上栄一
園芸学会平成26年度春季大会, 29 Mar. 2014, 園芸学会
20140329, 20140330

果皮色形質に連鎖するDNA マーカーSTS-OPH19 によるニホンナシの選抜と選抜有効性の検証　　　　　　　　　　　　　　　
池上秀利; 渡邉辰彦; 石坂晃; 桒原実; 松本和紀; 牛島孝策; 平島敬太; 井上栄一
園芸学会平成26年度春季大会, 29 Mar. 2014, 園芸学会

Isolation and characterization of the flowering-related genes in chestnut　　　　　　　　　　　　　　　
Eiichi Inoue; Tsunoda Suzuna; Takashi Kodama; Yasumasa Takatsu and Hisao Higashio
International ISHS Symposium on Physiological Principles and Their Application to Fruit Production, 27 Mar. 2014, ISHS
20140326, 20140328

マンゴスチン（Garcinia mangostana L.）におけるITS 領域の配列変異　　　　　　　　　　　　　　　
Matra Deden Derajat; 東尾久雄; 井上栄一
日本育種学会 第125回講演会 2014年度春季, 22 Mar. 2014, 日本育種学会
20140321, 20140322

QTL controlling dots development of fruit skin in Japanese pear　　　　　　　　　　　　　　　
Eiichi Inoue
Fruits and Roots: A Celebration and Forward Look, 07 Nov. 2013, Association of Applied Biologists
20131106, 20131107

S2 Progeny of Japanese Pear (Pyrus pyrifolia Nakai) ‘Osa Nijisseiki’ Having Self-compatibility and Their Homozygosity Estimated By SSR Analysis　　　　　　　　　　　　　　　
Eiichi Inoue
American Society for Horticultural Science, the 110th Annual Conference, 24 Jul. 2013, American Society for Horticultural Science
20130722, 20130725

Variation of nut characteristics in the interspecific hybrids between Japanese chastnut and Seguin chestnut　　　　　　　　　　　　　　　
岡部慎也; 井上栄一; 角田鈴奈; 高津康正; 東尾久雄
日本育種学会 第123回講演会 2013年春季, 28 Mar. 2013, 日本育種学会

クリにおける開花関連遺伝子の単離と解析　　　　　　　　　　　　　　　
角田鈴奈; 兒玉隆; 高津康正; 東尾久雄; 井上栄一
園芸学会平成25年度春季大会, 23 Mar. 2013, 園芸学会

徒長枝を用いたニホンナシ樹体における放射性セシウムの移行程度の評価　　　　　　　　　　　　　　　
飛田良憲; 高橋是成; 東尾久雄; 井上栄一
園芸学会平成25年度春季大会, 23 Mar. 2013, 園芸学会

クリ種間雑種におけるヨーロッパグリ由来SSRマーカーの遺伝　　　　　　　　　　　　　　　
岡部慎也; 井上栄一
平成２４年度果樹バイテク研究会, 01 Oct. 2012

Heredity of bush and parthenocapic trait in the progeny crossed between two fixed Tomato lines with those traits individually　　　　　　　　　　　　　　　
Tanaka, T; E. Inoue; T. Oyabu
園芸学会平成24年度秋季大会, 23 Sep. 2012, 園芸学会

Heredity of bush and parthenocapic trait in the progeny crossed,between two fixed Tomato lines with those traits individually　　　　　　　　　　　　　　　
Tanaka; T.; E. Inoue; T. Oyabu
園芸学会平成24年度秋季大会, 23 Sep. 2012, 園芸学会

Genetic Mapping and QTL Analysis in Interspecific Chestnut Population　　　　　　　　　　　　　　　
Eiichi Inoue
American Society for Horticultural Science, the 109th Annual Conference, 02 Aug. 2012, American Society for Horticultural Science

ヒマワリ園芸品種に及ぼすガンマ線急照射の影響　　　　　　　　　　　　　　　
馬場祐介; 川又有美; 小松明希奈; 山城なつ美; 山崎克己; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成24年度春季大会, Mar. 2012, 園芸学会

ニホンナシ果実のみつ症発生過程における組織学的観察　　　　　　　　　　　　　　　
小川達也; 大森裕樹; 本間貴司; 喜多晃一; 高津康正; 東尾久雄; 井上栄一
園芸学会平成24年度春季大会, Mar. 2012, 園芸学会

ブルーベリーのSSR マーカーによるスノキ属野生種の分類　　　　　　　　　　　　　　　
岡本 武; 鈴木太美雄; 櫛川 聡; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成24年度春季大会, Mar. 2012, 園芸学会

クリにおけるAP1 様遺伝子の単離と発現解析　　　　　　　　　　　　　　　
角田鈴奈; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成24年度春季大会, Mar. 2012, 園芸学会

Development of microsatellite marker for genetic analysis on mangosteen, Garcinia mangostana in Java Island　　　　　　　　　　　　　　　
Matra, D.D.・R. Poerwanto; Sobir・E. Santosa・E. Inoue
園芸学会平成24年度春季大会, Mar. 2012, 園芸学会

Iss education program "JAXA SpaceFlight Seeds Kids I"　　　　　　　　　　　　　　　
Tamotsu Nakano; Fumiaki Tanigaki; Eijiro Hirohama; Shiho Ogawa; Tomoko Abe; Eiichi Inoue; Yoriko Hayashi
62nd International Astronautical Congress, 2012

ニホングリ‘石鎚’とモーパングリの種間雑種における諸形質の解析　　　　　　　　　　　　　　　
角田鈴奈; 井上栄一
茨城県園芸談話会, 21 Nov. 2011, 茨城県園芸談話会

Growth characteristics and availability of sweet sorghum (Sorghum bicolor (L.) Moench) under non-irrigated field conditions in Ibaraki prefecture, Japan　　　　　　　　　　　　　　　
Tetsuya Oyabu; Tetsushi Tanaka; Yoshiyuki Asano and Eiichi Inoue
SOL & ICuGI，2011, Nov. 2011, SOL & ICuGI

ニホンナシ果実のみつ症発生過程における中性子線投影像の詳細な検討　　　　　　　　　　　　　　　
小川達也; 郷内武; 飯倉寛; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成23年度秋季大会, Sep. 2011, 園芸学会

ニホンナシにおける果皮色形質を制御するQTLの検出　　　　　　　　　　　　　　　
井上栄一; 西井瑞穂; 山本俊哉; 寺上伸吾
園芸学会平成23年度秋季大会, Sep. 2011, 園芸学会

ニホングリ‘石鎚’とクリ野生種モーパングリの種間雑種における形態及び開花特性の変異　　　　　　　　　　　　　　　
角田鈴奈; 佐々木道康; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成23年度秋季大会, Sep. 2011, 園芸学会

ミディトマトにおける柱頭切除を利用した単為結果性の選抜　　　　　　　　　　　　　　　
田中哲司; 井上栄一; 大藪哲也; 浅野義行
園芸学会平成23年度春季大会, Mar. 2011, 園芸学会

ニホングリとモーパングリの種間雑種を用いた遺伝学的連鎖地図の構築　　　　　　　　　　　　　　　
佐々木道康; 高津康正; 霞 正一; 本間貴司; 原 弘道; 東尾久雄; 井上栄一
園芸学会平成23年度春季大会, Mar. 2011, 園芸学会

ニホンナシ果実の‘みつ症’発生機構に関する研究(第5報)ニホンナシ‘豊水’みつ症組織に特異的なメタロチオネイン様タンパク質遺伝子の解析　　　　　　　　　　　　　　　
櫻井洋平; 江幡麻衣; 郷内武; 高津康正; 霞正一; 本間貴司; 東尾久雄; 井上栄一
園芸学会平成23年度春季大会, Mar. 2011, 園芸学会

スィートソルガム搾汁残渣の土壌への施用がコマツナの生育におよぼす影響　　　　　　　　　　　　　　　
本間貴司; 新田洋司; 浅木直美; 高柳浩一; 井上栄一; 成澤才彦; 久留主泰朗; 佐藤達雄; 加藤亮
園芸学会平成22年度秋季大会, Sep. 2010, 園芸学会

ニホンナシ果実の‘みつ症’発生機構に関する研究（第4報）各種サイトカイニンの持つみつ症抑制効果の検討　　　　　　　　　　　　　　　
櫻井洋平; 魚落紀子; 郷内武; 霞正一; 井上栄一
園芸学会平成22年度秋季大会, Sep. 2010, 園芸学会

自家和合性品種を利用したニホンナシS2世代の作出とSSRマーカーによるホモ化レベルの解析　　　　　　　　　　　　　　　
西山直紀; 郷内武; 井上栄一
園芸学会平成22年度秋季大会, Sep. 2010, 園芸学会

Time-course changes in the fine structure and chemical components of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) during the gelatinization process　　　　　　　　　　　　　　　
Homma; T.; H. Hara; T. Matsuda and E. Inoue
28th International Horticultural Congress, Aug. 2010, International Society for Horticultural Science (ISHS)

Isolation and characterization of metallothionein-like protein gene with differential expression in water core fruits in Japanese pear ‘Housui’　　　　　　　　　　　　　　　
Inoue; E.; M. Ebata; J. Y. Kim; Y. Sakurai; T. Gonai and M. Kasumi
28th International Horticultural Congress, Aug. 2010, International Society for Horticultural Science (ISHS)

Growth characteristics and availability of sweet sorghum (Sorghum bicolor (L.) Moench) under non-irrigated field conditions in Ibaraki prefecture, Japan　　　　　　　　　　　　　　　
Homma; T.; Y. Nitta; A. Kamiyama; N. Asagi; T. Matsuda; E. Inoue; K. Narisawa; Y. Kurusu; K. Watanabe; S. Oikawa; T. Yoshikawa; R. Kobayashi; R. Umehara
28th International Horticultural Congress, Aug. 2010, International Society for Horticultural Science (ISHS)

Genetic diversity in chestnut germplasm assessed by nuclear and chloroplast simple sequence repeat markers　　　　　　　　　　　　　　　
Inoue; E.; M. Kurabayashi; L. Ning; T. Homma and H. Hara
American Society for Horticultural Science, the 107th Annual Conference, Jul. 2010, American Society for Horticultural Science

ニホンナシ果実の‘みつ症’発生機構に関する研究（第3報）‘豊水’のみつ症組織で特異的に発現するメタロチオネイン様タンパク質遺伝子の特徴付け　　　　　　　　　　　　　　　
井上栄一; 江幡麻衣; 郷内武; 霞正一
園芸学会平成22年度春季大会, Mar. 2010, 園芸学会

生薬残渣の施用がコマツナおよびシソの生育に及ぼす影響　　　　　　　　　　　　　　　
山口雄一; 松本英樹; 三木栄二; 武田修己; 井上栄一
園芸学会平成22年度春季大会, Mar. 2010, 園芸学会

茨城県特産果樹のゲノム研究基盤の整備について　　　　　　　　　　　　　　　
井上栄一; 郷内武; 霞正一
シンポジウム 次世代シークエンサーとDNA塩基配列情報の農業研究への展開− 果樹ゲノム研究から品種識別まで −, 26 Feb. 2010, 茨城大学農学部研究推進委員会

ニホンナシの果皮色形質に連鎖するマーカーの開発　　　　　　　　　　　　　　　
井上栄一
果樹技術交流会, 09 Jan. 2010, 茨城県果樹研究会

ナシみつ症発生程度の異なる品種間および植物成長調節物質処理果実における中性子線投影像の差異　　　　　　　　　　　　　　　
郷内武; 井上栄一; 霞正一
園芸学会平成21年度秋季大会, 27 Sep. 2009, 園芸学会

Examination of the chromosome doubling condition in sweet sorghum　　　　　　　　　　　　　　　
E. Inoue; J. Nishigaki; Y. Nitta; T. Homma; T. Matsuda; K. Narisawa; Y. Kurusu; H. Ohta; S. Chonan; Y. Miyaguchi; A. Toyoda; T. Kato; H. Kobayashi; M. Komatsuzaki; T. Sato; K. Yoshida
日本育種学会 平成21年度秋季大会（第116回講演会）, 25 Sep. 2009, 日本育種学会

Interspecific Hybridization for Genetic Mapping in Chestnuts　　　　　　　　　　　　　　　
Eiichi Inoue; Michiyasu Sasaki; Takashi Homma; Masakazu Kasumi
Molecular Markers in Horticulture Symposium, 29 Jul. 2009, The Biotechnology Commission of the International Society for Horticultural Sciences (ISHS)

ニホンナシ自殖後代を利用したゲノム研究の展開　　　　　　　　　　　　　　　
井上栄一
平成２１年度果樹バイテク研究会, 25 Jun. 2009

Study on selection marker of pear. 7. Morphological quantification of fruit skin phenotypes for QTL analysis in F1 progeny of the Japanese pear ‘Niitaka’בChikusui’　　　　　　　　　　　　　　　
Y. Ohshima; T. Gonai; M. Kasumi; T. Homma; H. Hara; E. Inoue
園芸学会平成21年度春季大会, 20 Mar. 2009, 園芸学会

Analysis of SSR genotypes and phenotypes on an interspecific hybrid between Japanese chestnut and Seguin chestnut　　　　　　　　　　　　　　　
M. Sasaki; M. Kasumi; H. Hara; E. Inoue
園芸学会平成21年度春季大会, 19 Mar. 2009, 園芸学会

Study on selection marker of pear. 5. Co-dominant markers linked to fruit skin color phenotypes in Japanese pear.　　　　　　　　　　　　　　　
Ohshima; Y.; S. Terakami; T. Yamamoto; M.kasumi; T.Homma; H. Hara and E. Inoue
Autumn Meeting of Japanese Society of Horticultural Science, 2008, 28 Sep. 2008, 園芸学会

Study on selection marker of pear 6. Characterization of dots on the fruit among different fruit skin color ,genotypes in Japanese pear　　　　　　　　　　　　　　　
Homma; T.; Y. Ohshima; H. Hara and E. Inoue
Autumn Meeting of Japanese Society of Horticultural Science, 2008, 27 Sep. 2008, 園芸学会

Peeling easiness of nut pellicle in native Japanese (Castanea crenata Sieb. et Zucc.) and European (C. sativa Mill.) chestnuts evaluated by HOP method　　　　　　　　　　　　　　　
Inoue; E.; T. Homma; H. Hamada; N. Shiina; M. Ebata; Y. Kawamata; M. Kurabayashi; Y. Sakurai; M. Sasaki; H. Nakanishi and H. Hara
Autumn Meeting of Japanese Society of Horticultural Science, 2008, 27 Sep. 2008, 園芸学会

Study on watercore development in Japanese pear. 2. Specific gene on watercore tissues in Japanese pear ‘Housui’ selected by cDNA subtraction.　　　　　　　　　　　　　　　
Kim; J. Y.; T. Tateno; T. Gonai; M. Kasumi; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2008, 29 Mar. 2008, 園芸学会

Study on selection marker of pear. 4. Variation of fruit skin phenotypes evaluated by a morphological quantification among Japanese pear cultivars.　　　　　　　　　　　　　　　
Ohshima; Y.; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2008, 28 Mar. 2008, 園芸学会

ナシゲノム研究の発展と展望　　　　　　　　　　　　　　　
E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2008, Workshop, 27 Mar. 2008, 園芸学会

クリ果実の長期貯蔵中における品質要因の変化　　　　　　　　　　　　　　　
本間貴司; 根岸忠司; 小澤一男; 小松俊夫; 井上栄一; 松田智明; 原弘道
Autumn Meeting of Japanese Society of Horticultural Science, 2007, 30 Sep. 2007, 園芸学会

Genetic Comparison of Chestnuts and the Related Species in Fagaceae by cpSSR markers　　　　　　　　　　　　　　　
Eiichi Inoue; Lin Ning; Shuan Ruan; Hiroyuki Anzai and Hiromichi Hara
American Society for Horticultural Science, the 104th Annual Conference, 16 Jul. 2007, American Society for Horticultural Science

Genetic classification of chestnuts in Asia. 9. Genetic analysis of processed chestnuts by nuclear and chloroplast SSR markers.　　　　　　　　　　　　　　　
Hara; H.; L. Ning; H. Tsuzuki and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2007, 25 Mar. 2007, 園芸学会

Study on selection marker of pear. 3. Morphological analysis of fruit skin phenotypes for QTL analysis in Japanese pear.　　　　　　　　　　　　　　　
Ohshima; Y; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2007, 25 Mar. 2007, 園芸学会

Genetic classification of chestnuts in Asia. ７. Cross species amplification of SSR marker developed from chestnuts in Fagaceae species.　　　　　　　　　　　　　　　
Ning; L.; H. Hara; H. Tsuzuki; Y. Kitashima and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2007, 25 Mar. 2007, 園芸学会

Development and trial of the horticultural therapy for the psychiatric patients.　　　　　　　　　　　　　　　
Muramatsu; Y.; Y. Yamakawa; M. Nakagawa; M. Ikeda; S. Komatsuzaki; S. Yamaguchi; Y. Abe; T. Sasaki and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2007, 24 Mar. 2007, 園芸学会

Genetic classification of chestnuts in Asia. 8. Development of cultivar identification method on chestnuts by using the DNA extracted from the seed scar.　　　　　　　　　　　　　　　
Inoue; E.; L. Ning; H. Tsuzuki and H. Hara
Spring Meeting of Japanese Society of Horticultural Science, 2007, 24 Mar. 2007, 園芸学会

Genetic classification of chestnuts in Asia. 6. Interspecific hybridization between Japanese chestnut (C. crenata Sieb et Zucc.) and Seguin chestnut (C. seguinii Dode).　　　　　　　　　　　　　　　
Ning,L.; M. Kasumi; H. Tsuzuki; H. Hara and E. Inoue
Autumn Meeting of Japanese Society of Horticultural Science, 2006, 24 Sep. 2006, 園芸学会

Genetic Identification and Mapping on Pear by Microsatellite primers Developed in Several Rosaceae species.　　　　　　　　　　　　　　　
Eiichi Inoue; Celia James; Yumi Matsuki; Hiroyuki Anzai; Hiromichi Hara; Kate Evans
27th International Horticultural Congress & Exhibition, 2006, 14 Aug. 2006, International Society for Horticultural Science (ISHS)

Development and Analysis of Simple Sequence Repeats Markers in Chinese chestnut (Castanea mollissima Bl.).　　　　　　　　　　　　　　　
Ning Lin; Shuan Ruan; Yumi Matsuki; Hiroyuki Anzai; Hiromichi Hara and Eiichi Inoue
27th International Horticultural Congress & Exhibition, 2006, 14 Aug. 2006, International Society for Horticultural Science (ISHS)

Structural Analysis of Relationship between Fruit Quality and Heating Method in Japanese Chestnut (Castanea crenata Sieb. et Zucc.).　　　　　　　　　　　　　　　
Takeshi Honma; Hiromichi Hara; Eiichi Inoue and Toshiaki Matsuda
27th International Horticultural Congress & Exhibition, 2006, 14 Aug. 2006, International Society for Horticultural Science (ISHS)

Study on watercore development in Japanese pear. 1. Variation of watercore susceptibility among sib lines involving ‘Housui’.　　　　　　　　　　　　　　　
Kim; J. Y.; T. Gonai; T. Tateno; S. Nomura; M. Kasumi; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2006, 30 Mar. 2006, 園芸学会

Studies on histogenesis, transport system and matter accumulation in Chestnuts.　　　　　　　　　　　　　　　
Hara; H.; K. Koike; T. Mizutani; E. Inoue and T. Matsuda
Spring Meeting of Japanese Society of Horticultural Science, 2006, 30 Mar. 2006, 園芸学会

Genetic classification of chestnuts in Asia. 5. Genotypic analysis of chestnuts by chloroplast SSR markers.　　　　　　　　　　　　　　　
Ning,L.; S. Ruan; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2006, 29 Mar. 2006, 園芸学会

Study on selection marker of pear. 2. Development and cross-species amplification of SSR marker in Chinese quince (Chaenomeles sinensis ).　　　　　　　　　　　　　　　
Sanbongi; K.; N. Lin; H. Hara and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2006, 29 Mar. 2006, 園芸学会

ヤーコンおよびキクイモ１－ＳＳＴ遺伝子を導入した植物におけるフラクトオリゴ糖産生　　　　　　　　　　　　　　　
谷口美香; 武石愛佳; 永田裕加里; 井上栄一; 玉掛秀人; 安西弘行
日本農芸化学会2006年度大会, 27 Mar. 2006, 日本農芸化学会

ヤーコンのフラクトオリゴ糖生合成関連酵素の遺伝子単離　　　　　　　　　　　　　　　
永田裕加里; 谷口美香; 武石愛佳; 井上栄一; 安西弘行
日本農芸化学会2006年度大会, 26 Mar. 2006, 日本農芸化学会

Genetic classification of chestnuts in Asia. 3. Parentage analysis in Japanese chestnuts by SSR marker of Chinese chestnuts.　　　　　　　　　　　　　　　
Ning,L.; S. Ruan; H. Hara and E. Inoue
Autumn Meeting of Japanese Society of Horticultural Science, 2005, 02 Oct. 2005, 園芸学会

Genetic classification of chestnuts in Asia. 4. Analysis of null allele on the ICMA014 locus containing SSR of Chinese chestnuts.　　　　　　　　　　　　　　　
Inoue,E.; L. Ning; S. Ruan and H. Hara
Autumn Meeting of Japanese Society of Horticultural Science, 2005, 02 Oct. 2005, 園芸学会

Intergeneric hybrid between Japanese pear and apple. Confirmation of the hybridity by flow cytometry and SSR markers.　　　　　　　　　　　　　　　
Gonai; T.; T. Manabe; E. Inoue; M. Hayashi; T. Yamamoto; T. Hayashi; F. Sakuma and M. Kasumi
107th and 108th Meeting of Japanese Society of Breeding, 2005, 21 Aug. 2005, 日本育種学会

Analysis of transgene expression between transgenic rice with different ploidy levels.　　　　　　　　　　　　　　　
Shibata; H.; H. Anzai and E. Inoue
107th and 108th Meeting of Japanese Society of Breeding, 2005, 21 Aug. 2005, 日本育種学会

Cultivar identification of chinese chestnuts by SSR marker.　　　　　　　　　　　　　　　
Inoue; E.; L. Ning; S. Ruan and H. Hara
107th and 108th Meeting of Japanese Society of Breeding, 2005, 20 Aug. 2005, 日本育種学会

Genetic classification of chestnuts in Asia. 2. Development of SSR marker in Chinese chestnuts ‘Qianci’.　　　　　　　　　　　　　　　
Ning,L.; E. Inoue; S. Ruan; H. Anzai and H. Hara
Spring Meeting of Japanese Society of Horticultural Science, 2006, 04 Apr. 2005, 園芸学会

１－SST遺伝子導入によるテンサイ及びシロイヌナズナにおけるフラクトオリゴ糖産生　　　　　　　　　　　　　　　
谷口美香; 永田裕加里; 井上栄一; 玉掛秀人; 安西弘行
日本農芸化学会2005年度大会, 29 Mar. 2005, 日本農芸化学会

ヤーコンの貯蔵中におけるフラクトオリゴ糖分解とFFaseの関係　　　　　　　　　　　　　　　
永田裕加里; 谷口美香; 井上栄一; 安西弘行
日本農芸化学会2005年度大会, 29 Mar. 2005, 日本農芸化学会

Production and characterization of recombinant human lactoferrin in transgenic Javanica rice cv Rojolele　　　　　　　　　　　　　　　
Diah Rachmawati; Takuya Mori; Takehiko Hosaka; Fumio Takaiwa; Eiichi Inoue and Hiroyuki Anzai
4th International Crop Science Congress, Austrulia, 2004, 27 Sep. 2004

Gene expression of lactoferrin-derived antimicrobial peptides in rice　　　　　　　　　　　　　　　
Taku Funakoshi; Takehiko Hosaka; Eiichi Inoue and Hiroyuki Anzai
4th International Crop Science Congress, Austrulia, 2004, 27 Sep. 2004

Study on selection marker of pear. 1. Analysis of SSR loci of pear using SSR primer of Rosaceae plants.　　　　　　　　　　　　　　　
Inoue; E.; Y. Matsuki; C. James; K. Evans; H. Anzai and H. Hara
Autumn Meeting of Japanese Society of Horticultural Science, 2004, 26 Sep. 2004, 園芸学会

Various factor affect the food taste of the Sweet roasted chestnuts.　　　　　　　　　　　　　　　
Honma; T.; H. Hara; E. Inoue and T. Tsukihashi
Autumn Meeting of Japanese Society of Horticultural Science, 2004, 26 Sep. 2004, 園芸学会

Study of long term storage for Yacon tuberous roots.　　　　　　　　　　　　　　　
Uchida; Y.; T. Tsukihashi; E. Inoue and H. Hara
Autumn Meeting of Japanese Society of Horticultural Science, 2004, 26 Sep. 2004, 園芸学会

Genetic classification of chestnuts in Asia. 1. Genetic analysis of chestnuts in Korea using SSR primers of Japanese chestnuts.　　　　　　　　　　　　　　　
Ning,L.; E. Inoue; Y. Matsuki; S. Ruan; T. Yamamoto; H. Anzai and H. Hara
Autumn Meeting of Japanese Society of Horticultural Science, 2004, 26 Sep. 2004, 園芸学会

Analysis of haploid and doubled haploid in transgenic rice.　　　　　　　　　　　　　　　
Shibata; H.; C. Shimaya; T. Mori; H. Anzai and E. Inoue
106th Meeting of Japanese Society of Breeding, 2004, 22 Sep. 2004, 日本育種学会

Gene isolation of fructan biosynthetic enzymes, 1-FFT and FFase in Yacon (Sumallanthus Sonchifolius)　　　　　　　　　　　　　　　
Nagata; Y.; Y. Sunayama; M. Taniguchi; E. Inoue and H. Anzai
106th Meeting of Japanese Society of Breeding, 2004, 22 Sep. 2004, 日本育種学会

Relationship between taseting and heating process in Japanese chestnuts.　　　　　　　　　　　　　　　
Honma,T.; H. Hara; T. Tsukihashi and E. Inoue
Spring Meeting of Japanese Society of Horticultural Science, 2004, 04 Apr. 2004, 園芸学会

Isolation of full length cDNA of a fructan biosynthetic gene, 1-SST in Yacon (Sumallanthus Sonchifolius)　　　　　　　　　　　　　　　
Sunayama; Y.; Y. Nagata; N. Ito; E. Inoue and H. Anzai
105th Meeting of Japanese Society of Breeding, 2004, 31 Mar. 2004, 日本育種学会

Progeny analysis of transgenic rice Rojolele expressing recombinant human Lactoferrin.　　　　　　　　　　　　　　　
Rachmawati; D.; T. Hosaka; F. Takaiwa; E. Inoue and H. Anzai
105th Meeting of Japanese Society of Breeding, 2004, 31 Mar. 2004, 日本育種学会

Gene expression of lactoferrin-derived antimicrobial peptides in rice and functional analysis of their peptides.　　　　　　　　　　　　　　　
Funakoshi; T.; T. Hosaka; E. Inoue and H. Anzai
105th Meeting of Japanese Society of Breeding, 2004, 31 Mar. 2004, 日本育種学会

Effect of signal sequences on the expression of human Lactoferrin in transgenic rice.　　　　　　　　　　　　　　　
Hosaka; T.; T. Mori; M. Kabasawa; F. Takaiwa; E. Inoue and H. Anzai
105th Meeting of Japanese Society of Breeding, 2004, 31 Mar. 2004, 日本育種学会

Development of SSR marker in Japanese pear 'Niitaka'　　　　　　　　　　　　　　　
Matsuki; Y.; E. Inoue and H. Anzai
105th Meeting of Japanese Society of Breeding, 2004, 30 Mar. 2004, 日本育種学会

Jan. 2010, 茨城県果樹研究会

Japanese Society of Breeding

茨城県花き研究会

American Society for Horticultural Science

International Society for Horticultural Science

Japanese Society for Horticultural Science

Identification of candidate gene controlling cork formation on apple pericarp by using 'Gold farm' as a bud mutation variety of 'Fuji'
Grant-in-Aid for Scientific Research (C)
Ibaraki University
01 Apr. 2018 - 31 Mar. 2021

収穫後品質の向上と機能性を活かした加工品の展開による国産レンコンのブランド力強化プロジェクト
「革新的技術開発・緊急展開事業（うち地域戦略プロジェクト）
国立大学法人茨城大学
Oct. 2016 - Sep. 2019

Transcriptome analysis of watercore in Pyrus pyrifolia by comparing pairs of susceptible and resistant F1 sibs
Grant-in-Aid for Scientific Research (C)
National Agriculture and Food Research Organization
01 Apr. 2015 - 31 Mar. 2019

倍数性の異なるニホンナシ‘豊水’のマイクロアレイ解析によるみつ症原因遺伝子の探索　　　　　　　　　　　　　　　
挑戦的萌芽研究
Apr. 2014 - Mar. 2017

Genomic research and their utilization for selection marker development in Japanese chestnut
Grant-in-Aid for Scientific Research (B)
Ibaraki University
01 Apr. 2009 - 31 Mar. 2014

Study on inbreeding degeneration in Japanese pear by using S2 progeny originated from Osa Nijisseiki having self-compatibility
Grant-in-Aid for Challenging Exploratory Research
Ibaraki University
2011 - 2013

ニホンナシの'みつ症'多発系統に特異的な遺伝子発現および遺伝子産物の探索と解析
萌芽研究
茨城大学
2006 - 2008

バラ科果樹におけるSSR遺伝子座の分布と選抜マーカーとしての応用に関する研究
若手研究(B)
茨城大学
2003 - 2004

疑似戻し交配法によるニホンナシ連鎖地図の作製とQTL遺伝子座の推定
奨励研究(A)
茨城大学
2000 - 2001

ニホンナシにおける属間交雑によるアポミクシス誘発に関する研究
奨励研究(A)
茨城大学
1998 - 1999

特開2023-088799, 特許願2021-203757, 資化物及び資化物の製造方法
井上栄一、長谷川守文、豊田淳、藤井智教、柳橋 諒、大西賢一、磯山広行

特開2017-153370, 特願2016-036584, シイタケの品種識別法
井上栄一、田中徳夫

特開2012-147690, 特願2011-007006, ヤーコンシロップの製造方法及び該製造方法によって得られるヤーコンシロップ
宮口右二、石島繁、井上 栄一

特願2011-007006, ヤーコンシロップの製造方法及び該製造方法によって得られるヤーコンシロップ
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