Development of a new extraction method and functional analysis of phycocyanobilin from unique filamentous cyanobacteria
Jinichi Aoki; Takashi Yarita; Morifumi Hasegawa; Munehiko Asayama, Elsevier BV
Journal of Biotechnology, Aug. 2024, [Reviewed]

親水性-疎水性バランス型充填剤を用いる簡便な固相抽出精製による二枚貝中オカダ酸群の精密定量
鳥居塚南; 上原由理香; 長谷川守文; 渡辺卓穂; 鎗田孝
分析化学, May 2024, [Reviewed]

薬用シャクヤク栽培での中耕と草生管理の差異がペオニフロリン含有率と収量に及ぼす影響
関浩一; 長谷川守文; 小松﨑将一, 日本農作業学会
農作業研究, 20 Sep. 2021, [Reviewed]

Author Correction: Selection of a subspecies-specific diterpene gene cluster implicated in rice disease resistance.
Chuansong Zhan; Long Lei; Zixin Liu; Shen Zhou; Chenkun Yang; Xitong Zhu; Hao Guo; Feng Zhang; Meng Peng; Meng Zhang; Yufei Li; Zixin Yang; Yangyang Sun; Yuheng Shi; Kang Li; Ling Liu; Shuangqian Shen; Xuyang Wang; Jiawen Shao; Xinyu Jing; Zixuan Wang; Yi Li; Tomasz Czechowski; Morifumi Hasegawa; Ian Graham; Takayuki Tohge; Lianghuan Qu; Xianqing Liu; Alisdair R Fernie; Ling-Ling Chen; Meng Yuan; Jie Luo
Nature plants, Jan. 2021, [Reviewed]

Selection of a subspecies-specific diterpene gene cluster implicated in rice disease resistance
Chuansong Zhan; Long Lei; Zixin Liu; Shen Zhou; Chenkun Yang; Xitong Zhu; Hao Guo; Feng Zhang; Meng Peng; Meng Zhang; Yufei Li; Zixin Yang; Yangyang Sun; Yuheng Shi; Kang Li; Ling Liu; Shuangqian Shen; Xuyang Wang; Jiawen Shao; Xinyu Jing; Zixuan Wang; Yi Li; Tomasz Czechowski; Morifumi Hasegewa; Ian Graham; Takayuki Tohge; Lianghuan Qu; Xianqing Liu; Alisdair R. Fernie; Ling-Ling Chen; Meng Yuan; Jie Luo, Diterpenoids are the major group of antimicrobial phytoalexins in rice1,2. Here, we report the discovery of a rice diterpenoid gene cluster on chromosome 7 (DGC7) encoding the entire biosynthetic pathway to 5,10-diketo-casbene, a member of the monocyclic casbene-derived diterpenoids. We revealed that DGC7 is regulated directly by JMJ705 through methyl jasmonate-mediated epigenetic control3. Functional characterization of pathway genes revealed OsCYP71Z21 to encode a casbene C10 oxidase, sought after for the biosynthesis of an array of medicinally important diterpenoids. We further show that DGC7 arose relatively recently in the Oryza genus, and that it was partly formed in Oryza rufipogon and positively selected for in japonica during domestication. Casbene-synthesizing enzymes that are functionally equivalent to OsTPS28 are present in several species of Euphorbiaceae but gene tree analysis shows that these and other casbene-modifying enzymes have evolved independently. As such, combining casbene-modifying enzymes from these different families of plants may prove effective in producing a diverse array of bioactive diterpenoid natural products., Nature Research
Nature Plants, 07 Dec. 2020, [Reviewed]

Heat shock-induced resistance against Pseudomonas syringae pv. tomato (Okabe) Young et al. via heat shock transcription factors in tomato
Nur Akbar Arofatullah; Morifumi Hasegawa; Sayuri Tanabata; Isao Ogiwara; Tatsuo Sato, *Abstract: We investigated the role of heat shock transcription factors (Hsfs) during induction of defense response by heat-shock treatment (HST) in tomato. Leaf disease symptoms were significantly reduced at 12 and 24 h after HST, consistent with upregulation of pathogenesis-related (PR) genes PR1a2 and PR1b1 peaking at 24 h after treatment. These genes were upregulated at the treatment application site, but not in untreated leaves. In contrast to HST, inoculation of the first leaf induced systemic upregulation of acidic PR genes in uninoculated second leaves. Furthermore, heat shock element motifs were found in upstream regions of PR1a2, PR1b1, Chitinase 3, Chitinase 9, Glucanase A, and Glucanase B genes. Upregulation of HsfA2 and HsfB1 peaked at 6 h after HST, 6 h earlier than salicylic acid accumulation. Foliar spray of heat shock protein 90 (Hsp90) inhibitor geldanamycin (GDA) induced PR gene expression comparable to that after HST. PR gene expression and defense response against Pseudomonas syringae pv. tomato (Pst) decreased when combining HST with Hsfs inhibitor KRIBB11. The Hsfs and PR gene expression induced by heat or GDA, together with the suppression of heat shock-induced resistance (HSIR) against Pst by KRIBB11, suggested a direct contribution of Hsfs to HSIR regulation in tomato., MDPI
Agronomy, 11 Jan. 2019, [Reviewed]

Comparative analysis of the genetic basis of branched nonylphenol degradation by Sphingobium amiense DSM 16289^T and Sphingobium cloacae JCM 10874^T
Mina Ootsuka; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta, 日本微生物生態学会
Microbes and Environments, Dec. 2018, [Reviewed]

スィートソルガム栽培の有無と残渣の施用方法がウコン根茎の収量とクルクミン濃度におよぼす影響　　　　　　　　　　　　　　　
浅木直美; 窪田俊仁; 坂上伸生; 岡田拓也; 長谷川守文; 磯野健雄; 新田洋司, 日本農作業学会
農作業研究, Sep. 2018, [Reviewed]

Xylosylated detoxification of the rice flavonoid phytoalexin sakuranetin by the rice sheath blight fungus rhizoctonia solani
Shun Katsumata; Hiroaki Toshima; Morifumi Hasegawa, Sakuranetin (1) is a rice flavanone-type phytoalexin. We have already reported that the metabolites from the detoxification of 1 by Pyricularia oryzae are naringenin (2) and sternbin. In this study, we investigated whether the rice sheath blight fungus Rhizoctonia solani, another major rice pathogen, can detoxify 1. The extract of R. solani suspension culture containing 1 was analyzed by LC-MS to identify the metabolites of 1. Three putative metabolites of 1 were detected in the extract from the R. solani suspension culture 12 h after the addition of 1, and they were identified as 2, sakuranetin-4-O-β-D-xylopyranoside (3), and naringenin-7-O-β-D-xylopyranoside (4) by NMR, LC-MS/MS, and GC-MS analyses. The accumulation of 2, 3, and 4 reached their maximum levels 9–12 h after the addition of 1, whereas the content of 1 decreased to almost zero within 9 h. The antifungal activities of 3 and 4 against R. solani were negligible, and 2 showed weaker antifungal activity than 1. We concluded that 2, 3, and 4 are metabolites from the detoxification of 1 by R. solani. Xylosylation is a rare and efficient detoxification method for phytoalexins., MDPI AG
Molecules, 2018, [Reviewed]

Combining effects of ozone and Xanthomonas oryzae pv. oryzae on antioxidants and phytoalexins in rice (Oryza sativa L.)
Nithiwadee Buawat; Morifumi Hasegawa; Chanin Umponstira, Ozone is the most oxidative air pollutant and considered as an abiotic stress which harm to vegetation and crop. Similarly, biological stressor such as plant pathogen could cause severe damage to crops. Combination effects from both stresses will potentially lead to economic loss. Basically, plants will response to both abiotic stress and pathogenic stressor by generate antioxidants and phytoalexins. The concentration of these chemical indicate stress level in plant and also defence system response. This research is aimed to investigate the combination effects of ozone and Xanthomonas oryzae pv. oryzae on antioxidants and phytoalexins in rice (Oryza sativa L. cv. KDML 105). In this study, 45-day-old rice plants were used. Ozone was elevated at concentrations of 100, 200 and 300 ppb in the fumigating chambers for 12 hours. Xanthomonas oryzae pv. oryzae which caused bacterial blight disease in rice was selected as plant pathogen. Combining effects of ozone and Xanthomonas oryzae pv. oryzae were examined using ozone at a concentration of 100 ppb and Xanthomonas oryzae pv. oryzae 1x108 cells/ml inoculation. The experimental design was a completely randomized design with 2 × 2 factorials treatment and three replications required. Temperature, relative humidity and light intensity in fumigating chambers were controlled. The results of the combining effects showed the amount of antioxidants and phytoalexins were higher than those in single treatment. The experimental data indicated that the combining effects will result in more stress in rice. This suggests that, in rice plantation area with experience of ozone episode, pathogenic resistance rice strain should be considered to reduce combining effects., Southern Cross Publishing and Printing Pty Ltd
Australian Journal of Crop Science, 01 Dec. 2017, [Reviewed]

Identification of Sternbin and Naringenin as Detoxified Metabolites from the Rice Flavanone Phytoalexin Sakuranetin by Pyricularia oryzae
Shun Katsumata; Kazuho Hamana; Kiyotaka Horie; Hiroaki Toshima; Morifumi Hasegawa, Sakuranetin (1) is a flavanone phytoalexin that has been reported to play an important role in disease resistance in rice plants. The rice blast fungus Pyricularia oryzae (syn. Magnaporthe oryzae) has been reported to metabolize 1 to lower its antifungal activity. Here, two flavanones, sternbin (2) and naringenin (3), were identified as metabolites of 1 in P.oryzae suspension culture by liquid chromatography tandem mass spectrometry (LC/MS/MS). The inhibition of 1, 2, and 3 on P.oryzae mycelial growth were 45%, 19%, and 19%, respectively, at a concentration of 100m. Thus, 2 and 3 are detoxified metabolites of 1 by P.oryzae., WILEY-V C H VERLAG GMBH
CHEMISTRY & BIODIVERSITY, Feb. 2017, [Reviewed]

Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon
Tomonobu Toyomasu; Koji Miyamoto; Matthew R. Shenton; Arisa Sakai; Chizu Sugawara; Kiyotaka Horie; Hiroshi Kawaide; Morifumi Hasegawa; Masaru Chuba; Wataru Mitsuhashi; Hisakazu Yamane; Nori Kurata; Kazunori Okada, Cultivated rice (Oryza sativa) possesses various labdane-related diterpene synthase genes, homologs of ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) that are responsible for the biosynthesis of phytohormone gibberellins. The CPS homologs and KS like (KSL) homologs successively converted geranylgeranyl diphosphate to cyclic diterpene hydrocarbons via ent-copalyl diphosphate or syn-copalyl diphosphate in O. sativa. Consequently, a variety of labdane-related diterpenoids, including phytoalexin phytocassanes, momilactones and oryzalexins, have been identified from cultivated rice. Our previous report indicated that the biosynthesis of phytocassanes and momilactones is conserved in Oryza rufipogon, the progenitor of Asian cultivated rice. Moreover, their biosynthetic gene clusters, containing OsCPS2 and OsKSL7 for phytocassane biosynthesis and OsCPS4 and OsKSL4 for momilactone biosynthesis, are also present in the O. rufipogon genome. We herein characterized O. rufipogon homologs of OsKSL5, OsKSL6, OsKSL8 responsible for oryzalexin S biosynthesis, and OsKSL10 responsible for oryzalexins A-F biosynthesis, to obtain more evolutionary insight into diterpenoid biosynthesis in O. sativa. Our phytoalexin analyses showed that no accumulation of oryzalexins was detected in extracts from O. rufipogon leaf blades. In vitro functional analyses indicated that unlike OsKSL10, O. rufipogon KSL10 functions as an ent-miltiradiene synthase, which explains the lack of accumulation of oryzalexins A-F in O. rufipogon. The different functions of KSL5 and KSL8 in O. sativa japonica to those in indica are conserved in each type of O. rufipogon, while KSL6 functions (ent-isokaurene synthases) are well conserved. Our study suggests that O. sativa japonica has evolved distinct specialized diterpenoid metabolism, including the biosynthesis of oryzalexins. (C) 2016 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2016, [Reviewed]

Evolutionary trajectory of phytoalexin biosynthetic gene clusters in rice
Koji Miyamoto; Masahiro Fujita; Matthew R. Shenton; Shota Akashi; Chizu Sugawara; Arisa Sakai; Kiyotaka Horie; Morifumi Hasegawa; Hiroshi Kawaide; Wataru Mitsuhashi; Hideaki Nojiri; Hisakazu Yamane; Nori Kurata; Kazunori Okada; Tomonobu Toyomasu, John Wiley & Sons, Inc.
The Plant Journal, Aug. 2016, [Reviewed]

Evolutionary trajectory of phytoalexin biosynthetic gene clusters in rice
Koji Miyamoto; Masahiro Fujita; Matthew R. Shenton; Shota Akashi; Chizu Sugawara; Arisa Sakai; Kiyotaka Horie; Morifumi Hasegawa; Hiroshi Kawaide; Wataru Mitsuhashi; Hideaki Nojiri; Hisakazu Yamane; Nori Kurata; Kazunori Okada; Tomonobu Toyomasu, John Wiley & Sons, Inc.
The Plant Journal, Aug. 2016, [Reviewed]

Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity
Yumi Eguchi; Ani Widiastuti; Hiromitsu Odani; Yufita Dwi Chinta; Maki Shinohara; Hideyuki Misu; Haruna Kamoda; Tomofumi Watanabe; Morifumi Hasegawa; Tatsuo Sato, Enhanced antifungal activity by hot water treatment at 50 degrees C 20 s against Botrytis cinerea Pers. ex Fr., a gray mold pathogen was evaluated in thyme (Thymus vulgaris L.) Thymol, p-cymene, and gamma-terpinene were detected as the main volatile compounds in thyme plants. Emission of p-cymene and gamma-terpinene from thyme increased after treatment, whereas amount of thymol released did not change, suggested that existence of a thermo-dependent synthesis pathway of p-cymene and gamma-terpinene. Volatile p-cymene inhibited mycelial growth of pathogen at 0.50 mu L/ml, whereas gamma-terpinene had an inhibitory effect at 0.05, 0.10, and 0.50 mu L/ml. Transcript level of gamma-terpinene gene increased after 24 h of treatment. As a conclusion, heat treatment enhanced antifungal activity by volatile terpenoids synthesized by thermo-dependent synthesis pathway of them in thyme. (C) 2016 Elsevier Ltd. All rights reserved., ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, Apr. 2016, [Reviewed]

Ultraviolet-induced amides and casbene diterpenoids from rice leaves
Kiyotaka Horie; Kengo Sakai; Misaki Okugi; Hiroaki Toshima; Morifumi Hasegawa, To discover new phytoalexins, an 80% MeOH extract of UV-irradiated rice leaves was analyzed using LC-MS, resulting in the detection of three unidentified compounds. We isolated the compounds from the UV-irradiated rice leaves using chromatographic methods and identified the compounds as N-benzoyltyramine (1), and two casbene-type diterpenes, 5-dihydro-ent-10-oxodepresssin (2) and 5-deoxo-ent-10-oxodepressin (3), using spectroscopic methods. Additionally, we compared the accumulation levels of major UV-inducible compounds in response to Magnaporthe oryzae inoculation and the antifungal activities of the compounds against M. oryzae colony growth. Although 1-3 showed negligible antifungal activity against M. oryzae, the compounds significantly accumulated in M. oryzaeinoculated rice leaves. Furthermore, we confirmed that N-benzoyltryptamine and N-cinnamoyltryptamine also accumulated after M. oryzae inoculation and have relatively high antifungal activity against M. oryzae to the same extent as phytocassanes. These results strongly support the hypothesis that the two amides are rice phytoalexins. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
PHYTOCHEMISTRY LETTERS, Mar. 2016, [Reviewed]

Eucommicin A, a beta-truxinate lignan from Eucommia ulmoides, is a selective inhibitor of cancer stem cells
Ayaka Fujiwara; Mayuko Nishi; Shigeo Yoshida; Morifumi Hasegawa; Chieko Yasuma; Akihide Ryo; Yoshihito Suzuki, Cancer stem cells (CSCs) constitute a small population of undifferentiated cells within a tumor that have the ability to self-renew and drive tumor formation, thus behaving as cancer-initiating cancer cells. Therapeutic interventions that eliminate CSCs are necessary to completely cure patients, since CSCs are a crucial source of tumor recurrence and metastasis. An induced CSC-like (iCSCL) model was recently established using induced pluripotent stem cells (iPSCs). In this study, a natural product-eucommicin A-was identified from Eucommia ulmoides leaves by screening for anti-CSC activity using the iCSCL model. Its structure was elucidated by spectroscopic methods as a quinic acid diester of 3,4,3',4'-tetrahydroxy-beta-truxinic acid. Eucommicin A exhibited selective anti-CSC activity and inhibited tumor sphere formation by iCSCL cells. The results of this study suggest that eucommicin A could serve as a lead compound in the development of drugs to abrogate the sternness and self-renewal ability of CSCs. (C) 2015 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
PHYTOCHEMISTRY, Feb. 2016

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria
Sayaka Hondo; Masatoshi Takahashi; Takashi Osanai; Mami Matsuda; Tomohisa Hasunuma; Akio Tazuke; Yoichi Nakahira; Shigeru Chohnan; Morifumi Hasegawa; Munehiko Asayama, Genetic engineering and metabolite profiling for the overproduction of polyhydroxybutyrate (PHB), which is a carbon material in biodegradable plastics, were examined in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Transconjugants harboring cyanobacterial expression vectors that carried the pha genes for PHB biosynthesis were constructed. The overproduction of PHB by the engineering cells was confirmed through microscopic observations using Nile red, transmission electron microscopy (TEM), or nuclear magnetic resonance (NMR). We successfully recovered PHB from transconjugants prepared from nitrogen-depleted medium without sugar supplementation in which PHB reached approximately 7% (w/w) of the dry cell weight, showing a value of 12-fold higher productivity in the transconjugant than that in the control strain. We also measured the intracellular levels of acetyl-CoA, acetoacetyl-CoA, and 3-hydroxybutyryl-CoA (3HB-CoA), which are intermediate products for PHB. The results obtained indicated that these products were absent or at markedly low levels when cells were subjected to the steady-state growth phase of cultivation under nitrogen depletion for the overproduction of bioplastics. Based on these results, efficient factors were discussed for the overproduction of PHB in recombinant cyanobacteria. (C) 2015, The Society for Biotechnology, Japan. All rights reserved., SOC BIOSCIENCE BIOENGINEERING JAPAN
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Nov. 2015, [Reviewed]

Identification of UV-Induced Diterpenes Including a New Diterpene Phytoalexin, Phytocassane F, from Rice Leaves by Complementary GC/MS and LC/MS Approaches
Kiyotaka Horie; Yasuno Inoue; Miki Sakai; Qun Yao; Yosuke Tanimoto; Jinichiro Koga; Hiroaki Toshima; Morifumi Hasegawa, Rice phytoalexins are regarded as one of the most important weapons against pathogenic microorganisms. We attempted to identify novel phytoalexins and their derivatives using GC/MS and LC/MS analyses. Diterpene derivatives, 9 beta-pimara-7,15-diene-3 beta,6 beta,19-triol, 1, stemar-13-en-2 alpha-ol, 2, and 1 alpha,2 alpha-dihydroxy-ent-12,15-cassadiene-3,11-dione, 3, were isolated from UV-irradiated rice leaves by chromatographic methods. These structures were confirmed by 1D- and 2D-NMR and MS analyses. Interestingly, all three compounds were accumulated following an infection by the rice blast pathogen. Magnaporthe oryzae. Compounds 1 and 2 exhibited weak antifungal activity and may be the biosynthetic intermediates of rice phytoalexins momilactones and oryzalexin S, respectively. Compound 3 exhibited relatively high inhibitory activity against the fungal mycelial growth of M. oryzae to the same extent as the known phytoalexin phytocassane A. We conclude that 3 is a member of the cassane-type phytoalexin family and propose the name phytocassane F., AMER CHEMICAL SOC
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Apr. 2015

Adaptive significance of gall formation for a gall-inducing aphids on Japanese elm trees
Mami Takei; Sayaka Yoshida; Takashi Kawai; Morifumi Hasegawa; Yoshihito Suzuki, Insect galls are abnormal plant tissues induced by external stimuli from parasitizing insects. It has been suggested that the stimuli include phytohormones such as auxin and cytokinins produced by the insects. In our study on the role of hormones in gall induction by the aphid Tetraneura nigriabdominalis, it was found that feedback regulation related to auxin and cytokinin activity is absent in gall tissues, even though the aphids contain higher concentrations of those phytohormones than do plant tissues. Moreover, jasmonic acid signaling appears to be compromised in gall tissue, and consequently, the production of volatile organic compounds, which are a typical defense response of host plants to herbivory, is diminished. These findings suggest that these traits of the gall tissue benefit aphids, because the gall tissue is highly sensitive to auxin and cytokinin, which induce and maintain it. The induced defenses against aphid feeding are also compromised. The abnormal responsiveness to phytohormones is regarded as a new type of extended phenotype of gall-inducing insects. (C) 2014 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
JOURNAL OF INSECT PHYSIOLOGY, Jan. 2015, [Reviewed]

Analysis on Blast Fungus-Responsive Characters of a Flavonoid Phytoalexin Sakuranetin; Accumulation in Infected Rice Leaves, Antifungal Activity and Detoxification by Fungus
Morifumi Hasegawa; Ichiro Mitsuhara; Shigemi Seo; Kazunori Okada; Hisakazu Yamane; Takayoshi Iwai; Yuko Ohashi, To understand the role of the rice flavonoid phytoalexin (PA) sakuranetin for blast resistance, the fungus-responsive characteristics were studied. Young rice leaves in a resistant line exhibited hypersensitive reaction (HR) within 3 days post inoculation (dpi) of a spore suspension, and an increase in sakuranetin was detected at 3 dpi, increasing to 4-fold at 4 dpi. In the susceptible line, increased sakuranetin was detected at 4 dpi, but not at 3 dpi, by which a large fungus mass has accumulated without HR. Induced expression of a PA biosynthesis gene OsNOMT for naringenin 7-O-methyltransferase was found before accumulation of sakuranetin in both cultivars. The antifungal activity of sakuranetin was considerably higher than that of the major rice diterpenoid PA momilactone A in vitro and in vivo under similar experimental conditions. The decrease and detoxification of sakuranetin were detected in both solid and liquid mycelium cultures, and they took place slower than those of momilactone A. Estimated local concentration of sakuranetin at HR lesions was thought to be effective for fungus restriction, while that at enlarged lesions in susceptible rice was insufficient. These results indicate possible involvement of sakuranetin in blast resistance and its specific relation to blast fungus., MDPI AG
MOLECULES, Aug. 2014, [Reviewed]

Reverse-genetic approach to verify physiological roles of rice phytoalexins: characterization of a knockdown mutant of OsCPS4 phytoalexin biosynthetic gene in rice
Tomonobu Toyomasu; Masami Usui; Chizu Sugawara; Kazuko Otomo; Yuko Hirose; Akio Miyao; Hirohiko Hirochika; Kazunori Okada; Takafumi Shimizu; Jinichiro Koga; Morifumi Hasegawa; Masaru Chuba; Yoshiaki Kawana; Masaharu Kuroda; Eiichi Minami; Wataru Mitsuhashi; Hisakazu Yamane, A variety of labdane-related diterpenoids, including phytocassanes, oryzalexins and momilactones, were identified as phytoalexins in rice (Oryza sativa L.). Momilactone B was also isolated as an allelochemical exuded from rice roots. The biosynthetic genes of these phytoalexins have been identified, including six labdane-related diterpene cyclase genes such as OsCPS2, OsCPS4, OsKSL4, OsKSL7, OsKSL8 and OsKSL10. Here we identified an OsCPS4 knockdown mutant, cps4-tos, by screening Tos17 mutant lines using polymerase chain reaction. OsCPS4 encodes a syn-copalyl diphosphate synthase responsible for momilactones and oryzalexin S biosynthesis. Because Tos17 was inserted into the third intron of OsCPS4, the mature OsCPS4mRNA was detected in the cps4-tos mutant as well as the wild type. Nevertheless, mature OsCPS4 transcript levels in the cps4-tos mutant were about one sixth those in the wild type. The cps4-tos mutant was more susceptible to rice blast fungus than the wild type, possibly due to lower levels of momilactones and oryzalexin S in the mutant. Moreover, co-cultivation experiments suggested that the allelopathic effect of cps4-tos against some kinds of lowland weeds was significantly lower than that of the wild type, probably because of lower momilactone content exuded from cps4-tos roots. A reverse-genetic strategy using the cps4-tos mutant showed the possible roles of momilactones not only as phytoalexins but also as allelopathic substances., WILEY-BLACKWELL
PHYSIOLOGIA PLANTARUM, Jan. 2014, [Reviewed]

Heat shock-induced resistance in strawberry against crown rot fungus Colletotrichum gloeosporioides
Ani Widiastuti; Mioko Yoshino; Harufumi Saito; Keisuke Maejima; Songying Zhou; Hiromitsu Odani; Kazuhiko Narisawa; Morifumi Hasegawa; Youji Nitta; Tatsuo Sato, This present study investigated the effects of heat shock treatments in strawberry seedlings against crown rot caused by Colletotrichum gloeosporioides. Heat shock treatment at 50 degrees C for 20 s reduced the disease index of strawberry crown rot and increased chitinase 2-1 gene expression as well as free salycilic acid accumulation. Heat shock treatment did not reduce mycelial growth of C. gloeosporioides. BIT(2-benzisothiazol-3(2H)-one,1,1-dioxide) treatment could not protect strawberry seedlings against crown rot disease. This finding suggests that heat shock induces strawberry resistance against Colletotrichum crown rot and assumes that mechanisms other than SAR probably mediated the protective effect of heat shock-induced resistance. (C) 2013 Elsevier Ltd. All rights reserved., ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, Oct. 2013, [Reviewed]

Stereoselective Synthesis of a Promising Flower-Inducing KODA Analog, (9R,12S,13R,15Z)-9-Hydroxy-12,13-methylene-10-oxooctadec-15-enoic Acid
Shin Shimomura; Shuho Oyama; Kyohei Nakano; Morifumi Hasegawa; Hiroaki Toshima, Stereoselective synthesis of a promising flower-inducing 9,10-ketol octadecadienoic acid (KODA) analog, (9R,12S,13R,15Z)-9-hydroxy-12,13-methylene-10-oxooctadec-15-enoic acid, was designed to obtain the desired stereoisomer via coupling between chiral sulfone and aldehyde segments. A known chiral cyclopropane derivative was converted to the sulfone segment via carbon-chain elongation and sulfonylation. Dec-9-en-1-ol was converted to the aldehyde segment, whose C-9 configuration was introduced by Sharpless asymmetric dihydroxylation. Coupling of the both segments and subsequent assembly gave the desired (9R,12S,13R,15Z)analog. The (9S,12S,13R,15Z)-analog was also synthesized by using the enatiomeric aldehyde segment. This strategy made it possible to synthesize the remaining stereoisomeric analogs., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jun. 2013, [Reviewed]

Identification of a Novel Casbane-Type Diterpene Phytoalexin, ent-10-Oxodepressin, from Rice Leaves
Yasuno Inoue; Miki Sakai; Qun Yao; Yosuke Tanimoto; Hiroaki Toshima; Morifumi Hasegawa, A 70% methanol extract of UV-irradiated rice leaves (400 g) was separated by chromatographic methods to give UV-induced compound 1 (2.1 mg) which showed a possible molecular ion at m/z 300 in the GC/MS analysis. Its structure was determined by NMR and MS methods. The H-1- and C-13-NMR spectra of 1 were identical to those of 10-oxodepressin (2), a casbane-type diterpene derived from the soft coral, Sinularia depressa. The specific rotation of 1 was positive, whereas that of 2 was negative. We therefore established 1 as ent-10-oxodepressin. The accumulation of 1 was also induced by an inoculation of the rice blast fungus. Compound 1 inhibited spore germination (IC50 30 ppm) and germ tube growth (IC50 10 ppm) of the rice blast fungus. We thus concluded that 1 was a novel rice phytoalexin., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 2013, [Reviewed]

Heat shock-induced resistance increases chitinase-1 gene expression and stimulates salicylic acid production in melon (Cucumis melo L.)
Ani Widiastuti; Mioko Yoshino; Morifumi Hasegawa; Youji Nitta; Tatsuo Sato, The present study continued previous research to investigate a mechanism involved in heat shock-induced resistance (HSIR) of melon seedlings. HSIR in melon seedlings increased chitinase1 gene expression 12 and 72 h after treatment. Salicylic acid β-. d-glucoside and free salicylic acid showed significant accumulation in the leaves post-treatment, 24 and 96 h for SAG
and 96 h for free SA. We found that the treatment of heat shock on the first leaves reduced lesions of gray mold on the untreated second leaves. It was assumed that SAR occurred in HSIR, however it was not as the first mechanism. © 2013 Elsevier Ltd.
Physiological and Molecular Plant Pathology, Apr. 2013, [Reviewed]

The potential bioproduction of the pharmaceutical agent sakuranetin, a flavonoid phytoalexin in rice
Takafumi Shimizu; Fengqiu Lin; Morifumi Hasegawa; Hideaki Nojiri; Hisakazu Yamane; Kazunori Okada
Bioengineered, Nov. 2012, [Reviewed]

Phytohormones and willow gall induction by a gall-inducing sawfly
Hiroki Yamaguchi; Hiroki Tanaka; Morifumi Hasegawa; Makoto Tokuda; Tadao Asami; Yoshihito Suzuki, A variety of insect species induce galls on host plants. Several studies have implicated phytohormones in insect-induced gall formation. However, it has not been determined whether insects can synthesize phytohormones. It has also never been established that phytohormones function in gall tissues. Liquid chromatography and tandem mass spectrometry (LC/MS/MS) were used to analyse concentrations of endogenous cytokinins and the active auxin IAA in the gall-inducing sawfly (Pontania sp.) and its host plant, Salix japonica. Feeding experiments demonstrated the ability of sawfly larvae to synthesize IAA from tryptophan. Gene expression analysis was used to characterize hormonal signalling in galls. Sawfly larvae contain high concentrations of IAA and t-zeatin, and produce IAA from tryptophan. The glands of adult sawflies, the contents of which are injected into leaves upon oviposition and are involved in the initial stages of gall formation, contain an extraordinarily high concentration of t-zeatin riboside. Transcript levels of some auxin- and cytokinin-responsive genes are significantly higher in gall tissue than in leaves. The abnormally high concentration of t-zeatin riboside in the glands strongly suggests that the sawfly can synthesize cytokinins as well as IAA. Gene expression profiles indicate high levels of auxin and cytokinin activities in growing galls., WILEY-BLACKWELL
NEW PHYTOLOGIST, Oct. 2012, [Reviewed]

Purification and Identification of Naringenin 7-O-Methyltransferase, a Key Enzyme in Biosynthesis of Flavonoid Phytoalexin Sakuranetin in Rice
Takafumi Shimizu; Fengqiu Lin; Morifumi Hasegawa; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane, Sakuranetin, the major flavonoid phytoalexin in rice, is induced by ultraviolet (UV) irradiation, CuCl2 treatment, jasmonic acid treatment, and infection by phytopathogens. It was recently demonstrated that sakuranetin has anti-inflammatory activity, anti-mutagenic activity, anti-pathogenic activities against Helicobacter pylori, Leishmania, and Trypanosoma and contributes to the maintenance of glucose homeostasis in animals. Thus, sakuranetin is a useful compound as a plant antibiotic and a potential pharmaceutical agent. Sakuranetin is biosynthesized from naringenin by naringenin 7-O-methyltransferase (NOMT). In previous research, rice NOMT (OsNOMT) was purified to apparent homogeneity from UV-treated wild-type rice leaves, but the purified protein, named OsCOMT1, exhibited caffeic acid O-methyltransferase (COMT) activity and not NOMT activity. In this study, we found that OsCOMT1 does not contribute to sakuranetin production in rice in vivo, and we purified OsNOMT using the oscomt1 mutant. A crude protein preparation from UV-treated oscomt1 leaves was subjected to three sequential purification steps, resulting in a 400-fold purification from the crude enzyme preparation. Using SDS-PAGE, the purest enzyme preparation showed a minor band at an apparent molecular mass of 40 kDa. Two O-methyltransferase-like proteins, encoded by Os04g0175900 and Os12g0240900, were identified from the 40-kDa band by MALDI-TOF/TOF analysis. Recombinant Os12g0240900 protein showed NOMT activity, but the recombinant Os04g0175900 protein did not. Os12g0240900 expression was induced by jasmonic acid treatment in rice leaves prior to sakuranetin accumulation, and the Os12g0240900 protein showed reasonable kinetic properties to OsNOMT. On the basis of these results, we conclude that Os12g0240900 encodes an OsNOMT., AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 2012, [Reviewed]

Identification of a Degradation Intermediate of the Momilactone A Rice Phytoalexin by the Rice Blast Fungus
Takuya Imai; Yuko Ohashi; Ichiro Mitsuhara; Shigemi Seo; Hiroaki Toshima; Morifumi Hasegawa, We have already shown that major rice diterpene phytoalexin, momilactone A, was detoxified by Magnaporthe oryzae. We report here the identification by NMR, MS, and chemical synthesis of 3,6-dioxo-19-nor-9 beta-pimara-7,15-diene (1) as the degradation intermediate. Compound 1 exhibited similar antifungal activity to that of momilactone A, indicating 1 to be a precursor of possible detoxified compounds., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2012, [Reviewed]

Accumulation of salicylic acid, jasmonic acid and phytoalexins in rice, Oryza sativa, infested by the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)
Hiroo Kanno; Morifumi Hasegawa; Osamu Kodama, In order to clarify the mechanism of induced resistance to blast disease in rice, Oryza sativa, that had been previously infested by the white-backed planthopper, Sogatella furcifera Horvath, we first investigated the accumulation of salicylic acid (SA) and jasmonic acid (JA) in rice plants infested by the planthopper. The results confirmed that infestation of S. furcifera strongly stimulates the production of SA and JA in rice. These results indicate that both salicylate- and jasmonate-mediated pathways (SA and JA pathways), which are involved in the general defense system in plants, were activated in rice infested by S. furcifera. Further results confirmed that S. furcifera infestation induces accumulation of a major rice diterpenoid phytoalexin, momilactone A, and a flavonoid phytoalexin, sakuranetin, which are well known as antimicrobial chemicals, particularly in blast disease caused by the blast fungus, Magnaporthe oryzae B. Couch. All these results strongly suggest the following hypothetical mechanism of induced-resistance to M. oryzae in rice infested by S. furcifera. First, S. furcifera releases some elicitor-active compounds, which might be produced in the salivary glands, into the rice plant during feeding. Next, the defense signal systems, SA- and JA-mediated pathways, are activated by the elicitor. Finally, phytoalexins are induced in rice as antimicrobial compounds mainly through activation of the JA-mediated pathway., SPRINGER TOKYO
APPLIED ENTOMOLOGY AND ZOOLOGY, Feb. 2012, [Reviewed]

キュウリに病害抵抗性を誘導する熱ショック処理方法の改良と処理装置の試作　　　　　　　　　　　　　　　
芳野未央子; アニ・ウィディアストゥティ; 周松嬰; 小谷博光; 長谷川守文; 佐藤達雄
園芸学研究, Feb. 2012, [Reviewed]

Biomimetic Cyclization of Epoxide Precursors of Indole Mono-, Sesqui- and Diterpene Alkaloids by Lewis Acids
Tetsuya Isaka; Morifumi Hasegawa; Hiroaki Toshima, Cyclization of the synthesized epoxide precursors of indole mono-, sesqui- and diterpene alkaloids was performed to elucidate the mechanism for biomimetic cationic cyclization to polycyclic structures. 3-(6,7-Epoxygeranyl)indole (11), 3-(10,11-epoxyfarnesyl)indole (2) and 3-(14,15-epoxygeranylgeranyl)indole (3) were respectively synthesized from geraniol, farnesol and geranylgeraniol in 6 or 7 steps. Four Lewis acids (MeAlCl2, BF3 center dot OEt2, TiCl4 and SnCl4) were applied for biomimetic cyclization of the synthesized epoxide precursors. The cyclization products (one product from 11, four products from 2, and three products from 3) were isolated after separation by chromatography. Their structures were determined by using NMR (COSY, HSQC, HMBC, NOESY, etc.) and HRMS analyses. The results show that biomimetic cyclization gave new polycyclic compounds similar to natural indole terpene alkaloids. We conclude that the stability of cation intermediates should determine the preference for product formation by biomimetic cyclization when using a Lewis acid., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2011, [Reviewed]

温湯浸漬を用いた熱ショックにより誘導されるキュウリの灰色かび病抵抗性とその機作
芳野未央子; アニ・ウィディアストゥティ; 長谷川守文; 佐藤達雄, The optimum conditions and mechanisms of inducing disease resistance by heat shock were investigated against gray mold (Botrytis cinerea) in cucumber (Cucumis sativus L.). The growth of inoculated mycelium was controlled most effectively by treatment at 40°C, for 2 minutes. Salicylic acid accumulated in the treated leaves under this condition and two peaks were obtained 24 and 96 hours after treatment. A pathogenesis-related protein, peroxidase gene (POX; M91372), was also expressed in the treated leaves and showed two peaks at 24 and 96 hours, respectively after treatment. Partial heat shock treatment was conducted by dipping only the first leaf of two leaves seedlings. Resistance and POX expression were induced not only in the treated leaves but also in the untreated second leaves. In conclusion, heat shock by means of hot water dipping could induce resistance against gray mold in cucumber. Systemic acquired resistance may have been obtained by some possible pathway.
, THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
園芸学研究, 23 Aug. 2011, [Reviewed]

Stereocontrolled total synthesis of (+/-)-3 beta-hydroxy-9 beta-pimara-7,15-diene, a putative biosynthetic intermediate of momilactones
Arata Yajima; Kou Toda; Kazunori Okada; Hisakazu Yamane; Masamichi Yamamoto; Morifumi Hasegawa; Ryo Katsuta; Tomoo Nukada, The total synthesis of (+/-)-3 beta-hydroxy-9 beta-pimara-7,15-diene (1), a putative biosynthetic intermediate of the momilactones, was accomplished stereoselectively. Our methodology for the synthesis of 1 featured the stereoselective construction of the C-13 quaternary carbon center via the aldol-Tishchenko reaction. 3 beta-Hydroxy-9 beta-pimara-7,15-diene (1) was identified by full-scan CC-MS analysis as an endogenous compound in elicited rice cells. (C) 2011 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
TETRAHEDRON LETTERS, Jun. 2011, [Reviewed]

Induction of disease resistance against Botrytis cinerea by heat shock treatment in melon (Cucumis melo L.)
Ani Widiastuti; Mioko Yoshino; Harufumi Saito; Keisuke Maejima; Songying Zhou; Hiromitsu Odani; Morifumi Hasegawa; Youji Nitta; Tatsuo Sato, The present study investigated resistance against Botrytis cinerea after heat shock treatment in melon plants. Heat shock at 50 degrees C for 20 s 0-24 h before inoculation resulted in maximal B. cinerea symptom reduction and peroxidase gene expression, which peaked 12 and 72 h post-treatment and decreased 24-48 h post-treatment, suggesting pathogenesis-related protein expression priming. Hot water dipping did not directly inhibit mycelia growth. Plants treated with 2-benzisothiazol-3(2H)-one 1,1-dioxide, which induces systemic acquired resistance, demonstrated higher peroxidase gene expression but no B. cinerea resistance, indicating possible involvement of additional novel mechanisms in heat shock-activated resistance of melon against B. cinerea. (C) 2011 Elsevier Ltd. All rights reserved., ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, Apr. 2011, [Reviewed]

Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol
Yoko Ikunaga; Ikuo Sato; Stephanie Grond; Nobutaka Numaziri; Shigenobu Yoshida; Hiroko Yamaya; Syuntaro Hiradate; Morifumi Hasegawa; Hiroaki Toshima; Motoo Koitabashi; Michihiro Ito; Petr Karlovsky; Seiya Tsushima, The mycotoxin deoxynivalenol (DON) causes serious problems worldwide in the production of crops such as wheat and barley because of its toxicity toward humans and livestock. A bacterial culture capable of degrading DON was obtained from soil samples collected in wheat fields using an enrichment culture procedure. The isolated bacterium, designated strain WSN05-2, completely removed 1,000 mu g/mL of DON from the culture medium after incubation for 10 days. On the basis of phylogenetic studies, WSN05-2 was classified as a bacterium belonging to the genus Nocardioides. WSN05-2 showed significant growth in culture medium with DON as the sole carbon source. High-performance liquid chromatography analysis indicated the presence of a major initial metabolite of DON in the culture supernatant. The metabolite was identified as 3-epi-deoxynivalenol (3-epi-DON) by mass spectrometry and (1)H and (13)C nuclear magnetic resonance analysis. The amount of DON on wheat grain was reduced by about 90% at 7 days after inoculation with WSN05-2. This is the first report of a Nocardioides sp. strain able to degrade DON and of the yet unknown 3-epi-DON as an intermediate in the degradation of DON by a microorganism., SPRINGER
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Jan. 2011, [Reviewed]

Cyanide, a Coproduct of Plant Hormone Ethylene Biosynthesis, Contributes to the Resistance of Rice to Blast Fungus
Shigemi Seo; Ichiro Mitsuhara; Jiao Feng; Takayoshi Iwai; Morifumi Hasegawa; Yuko Ohashi, Rice (Oryza sativa) plants carrying the Pi-i resistance gene to blast fungus Magnaporthe oryzae restrict invaded fungus in infected tissue via hypersensitive reaction or response (HR), which is accompanied by rapid ethylene production and formation of small HR lesions. Ethylene biosynthesis has been implicated to be important for blast resistance; however, the individual roles of ethylene and cyanide, which are produced from the precursor 1-aminocyclopropane-1-carboxylic acid, remain unevaluated. In this study, we found that Pi-i-mediated resistance was compromised in transgenic rice lines, in which ethylene biosynthetic enzyme genes were silenced and then ethylene production was inhibited. The compromised resistance in transgenic lines was recovered by exogenously applying cyanide but not ethephon, an ethylene-releasing chemical in plant tissue. In a susceptible rice cultivar, treatment with cyanide or 1-aminocyclopropane-1-carboxylic acid induced the resistance to blast fungus in a dose-dependent manner, while ethephon did not have the effect. Cyanide inhibited the growth of blast fungus in vitro and in planta, and application of flavonoids, secondary metabolites that exist ubiquitously in the plant kingdom, enhanced the cyanide-induced inhibition of fungal growth. These results suggested that cyanide, whose production is triggered by HR in infected tissue, contributes to the resistance in rice plants via restriction of fungal growth., AMER SOC PLANT BIOLOGISTS
PLANT PHYSIOLOGY, Jan. 2011, [Reviewed]

Phytoalexin Accumulation in the Interaction Between Rice and the Blast Fungus
Morifumi Hasegawa; Ichiro Mitsuhara; Shigemi Seo; Takuya Imai; Jinichiro Koga; Kazunori Okada; Hisakazu Yamane; Yuko Ohashi, Blast fungus induced accumulations of major rice diterpene phytoalexins (PA), momilactones A and B, and phytocassanes A through E were studied, focusing on their biosynthesis and detoxification. In resistant rice, all PA started to accumulate at 2 days postinoculation (dpi), at which hypersensitive reaction (HR)-specific small lesions became visible and increased 500- to 1,000-fold at 4 dpi, while the accumulation was delayed and several times lower in susceptible rice. Expression of PA biosynthetic genes was transiently induced at 2 dpi only in resistant plants, while it was highly induced in both plants at 4 dpi. Fungal growth was severely suppressed in resistant plants by 2 dpi but considerably increased at 3 to 4 dpi in susceptible plants. Momilactone A treatment suppressed fungal growth in planta and in vitro, and the fungus detoxified the PA in vitro. These results indicate that HR-associated rapid PA biosynthesis induces severe restriction of fungus, allowing higher PA accumulation in resistant rice, while in susceptible rice, failure of PA accumulation at the early infection stage allows fungal growth. Detoxification of PA would be a tactic of fungus to invade the host plant, and prompt induction of PA biosynthesis upon HR would be a trait of resistant rice to restrict blast fungus., AMER PHYTOPATHOLOGICAL SOC
MOLECULAR PLANT-MICROBE INTERACTIONS, Aug. 2010, [Reviewed]

SYNTHESIS OF OPTICALLY ACTIVE gamma-VALEROLACTONE AND gamma-NONANOLACTONE VIA OPTICAL RESOLUTION USING CHIRAL AMINE DERIVED FROM AMINO ACID
Kenichi Yumoto; Morifumi Hasegawa; Hiroaki Toshima, Optically active gamma-valerolactone and gamma-nonanolactone have been synthesized via optical resolution using a newly developed chiral airline derived from L-phenylalanine. Both racemic gamma-lactones were transformed to corresponding diastereomeric amides by amidation with the optical resolution agent. Fractional crystallization of diastereomeric amides, recrystallization of each diastereomer, and Subsequent hydrolysis gave optically active gamma-valerolactone and gamma-nonanolactone with Sufficient enantiomeric excess and isolated yield. The optical resolution agent was recovered after hydrolysis., PERGAMON-ELSEVIER SCIENCE LTD
HETEROCYCLES, Feb. 2010, [Reviewed]

Contribution of momilactone A and B to rice allelopathy
Hisashi Kato-Noguchi; Morifumi Hasegawa; Takeshi Ino; Katsumi Ota; Hiroya Kujime, Eight cultivars of rice (Oryza sativa L.) inhibited shoot and root growth of Echinochloa crus-galli when co-cultured with rice seedlings in a bioassay medium. Momilactone A and B were found in the bioassay medium of all rice cultivars, and concentrations of momilactone A and B in the medium were 0.21-1.5 and 0.66-3.8 mu mol/L, respectively, indicating that all rice cultivars may secrete momilactone A and B into the medium. Exogenously applied momilactone A and B inhibited the growth of shoots and roots of E. crus-galli at concentrations greater than 30 and 1 mu mol/L, respectively. The concentrations required for 50% growth inhibition of E. crus-galli shoots and roots, respectively, were 146 and 91 mu mol/L for momilactone A and 6.5 and 6.9 mu mol/L for momilactone B. Considering the growth inhibitory activity and concentrations found in the bioassay medium, momilactone A may have caused only 0.8-2.2% of the observed growth inhibition of E. crus-galli roots and shoots by rice. However, momilactone B in the medium was estimated to be able to cause 59-82% of the observed growth inhibition of E. crus-galli roots and shoots by the rice seedlings. In addition, the concentrations of momilactone B in the medium reflected the observed differences in the growth inhibition of E. crus-galli by the eight rice cultivars investigated. This suggests that the allelopathic activity of rice may depend primarily on the secretion level of momilactone B. Therefore, momilactone B may play a very important role in rice allelopathy. (C) 2010 Elsevier GmbH. All rights reserved., ELSEVIER GMBH, URBAN & FISCHER VERLAG
JOURNAL OF PLANT PHYSIOLOGY, 2010, [Reviewed]

Suppression of the Rice Fatty-Acid Desaturase Gene OsSSI2 Enhances Resistance to Blast and Leaf Blight Diseases in Rice
Chang-Jie Jiang; Masaki Shimono; Satoru Maeda; Haruhiko Inoue; Masaki Mori; Morifumi Hasegawa; Shoji Sugano; Hiroshi Takatsuji, Fatty acids and their derivatives play important signaling roles in plant defense responses. It has been shown that suppressing a gene for stearoyl acyl carrier protein fatty-acid desaturase (SACPD) enhances the resistance of Arabidopsis (SSI2) and soybean to multiple pathogens. In this study, we present functional analyses of a rice homolog of SSI2 (OsSSI2) in disease resistance of rice plants. A transposon insertion mutation (Osssi2-Tos17) and RNAi-mediated knockdown of OsSSI2 (OsSSI2-kd) reduced the oleic acid (18:1) level and increased that of stearic acid (18: 0), indicating that OsSSI2 is responsible for fatty-acid desaturase activity. These plants displayed spontaneous lesion formation in leaf blades, retarded growth, slight increase in endogenous free salicylic acid (SA) levels, and SA/benzothiadiazole (BTH)-specific inducible genes, including WRKY45, a key regulator of SA/BTH-induced resistance, in rice. Moreover, the OsSSI2-kd plants showed markedly enhanced resistance to the blast fungus Magnaporthe grisea and leaf-blight bacteria Xanthomonas oryzae pv. oryzae. These results suggest that OsSSI2 is involved in the negative regulation of defense responses in rice, as are its Arabidopsis and soybean counterparts. Microarray analyses identified 406 genes that were differentially expressed (>= 2-fold) in OsSSI2-kd rice plants compared with wild-type rice and, of these, approximately 39% were BTH responsive. Taken together, our results suggest that induction of SA-responsive genes, including WRKY45, is likely responsible for enhanced disease resistance in OsSSI2-kd rice plants., AMER PHYTOPATHOLOGICAL SOC
MOLECULAR PLANT-MICROBE INTERACTIONS, Jul. 2009, [Reviewed]

Resistance to Magnaporthe grisea in transgenic rice with suppressed expression of genes encoding allene oxide cyclase and phytodienoic acid reductase
Asanori Yara; Takashi Yaeno; Morifumi Hasegawa; Hideharu Seto; Shigemi Seo; Kensuke Kusumi; Koh Iba, Linolenic acid (18:3) and its derivative jasmonic acid (]A) are important molecules in disease resistance in many dicotyledonous plants. We have previously used 18:3- and JA-deficient rice (F78Ri) to investigate the roles of fatty acids and their derivatives in resistance to the blast fungus Magnaporthe grisea JA. Yara, T. Yaeno, J.-L. Montillet, M. Hasegawa, S. Seo, K. Kusumi, K. Iba, Enhancement of disease resistance to Magnaporthe grisea in rice by accumulation of hydroxy linoleic acid, Biochem. Biophys. Res. Commun. 370 (2008) 344-347; A. Yara, T. Yaeno, M. Hasegawa, H. Seto, J.-L. Montillet, K. Kusumi, S. Seo, K. Iba, Disease resistance against Magnaporthe grisea is enhanced in transgenic rice With Suppression of omega-3 fatty acid desaturases, Plant Cell Physiol. 48 (2007) 1263-1274]. However, because F78Ri plants are suppressed in the first step of the JA biosynthetic pathway, we could not confirm the specific contribution of JA to disease resistance. In this paper, we generated two JA-deficient rice lines (AOCRi and OPRRi) with suppressed expression of the genes encoding allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR), which catalyze late steps in the JA biosynthetic pathway. The levels of disease resistance in the AOCRi and OPRRi lines were equal to that in wild-type plants. Our data suggest that resistance to M. grisea is not dependent on JA synthesis. (c) 2008 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2008, [Reviewed]

Enhancement of disease resistance to Magnaporthe grisea in irice by accumulation of hydroxy linoleic acid
Asanori Yara; Takashi Yaeno; Jean-Luc Montillet; Morifumi Hasegawa; Shigemi Seo; Kensuke Kusumi; Koh Iba, Linoleic acid (18:2) and linolenic acid (18:3) are sources for various oxidized metabolites called oxylipins, some of which inhibit growth of fungal pathogens. In a previous study, we found disease resistance to rice blast fungus Magnaporthe grisea enhanced in 18:2-accumulating transgenic rice (F78Ri) in which the conversion from 18:2 to 18:3 was suppressed. Here, we demonstrate that 18:2-derived hydroperoxides and hydroxides (HPODEs and HODEs, respectively) inhibit growth of M. grisea more strongly than their 18:3-derived counterparts. Furthermore, in F78Ri plants, the endogenous levels of HPODEs and HODEs increased significantly, compared with wild-type plants. These results suggest that the increased accumulation of antifungal oxylipins, such as HPODEs and HODEs, causes the enhancement of disease resistance against M. grisea. (c) 2008 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2008, [Reviewed]

Diterpene phytoalexins are biosynthesized in and exuded from the roots of rice seedlings
Tomonobu Toyomasu; Takuma Kagahara; Kazunori Okada; Jinichiro Koga; Morifumi Hasegawa; Wataru Mitsuhashi; Takeshi Sassa; Hisakazu Yamane, Rice (Oryza sativa L.) produces a variety of diterpene phytoalexins, such as momilactones, phytocassanes, and oryzalexins. Momilactone B was previously identified as an allelopathic substance exuded from the roots of rice. We identified in this present study momilactone A and phytocassanes A-E in extracts of, and exudates from, the roots of rice seedlings. The concentration of each compound was of the same order of magnitude as that of momilactone B. Expression analyses of the diterpene cyclase genes responsible for the biosynthesis of momilactones and phytocassanes suggest that these phytoalexins found in roots are primarily biosynthesized in those roots. None of phytocassanes B-E exhibited allelopathic activity against dicot seedling growth, whereas momilactone A showed much weaker allelopathic activity than momilactone B. The exudation of diterpene phytoalexins from the roots might be part of a system for defense against root-infecting pathogens., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2008, [Reviewed]

Effects of the bile acid elicitor, cholic acid, on the biosynthesis of diterpenoid phytoalexins in suspension-cultured rice cells
Takafumi Shimizu; Yusuke Jikumaru; Atsushi Okada; Kazunori Okada; Jinichiro Koga; Kenji Umemura; Naoto Shibuya; Morifumi Hasegawa; Osamu Kodama; Hideaki Nojiri; Hisakazu Yamane, An elicitor of rice defense responses was recently isolated from human feces and was identified as cholic acid (CA). Pathogen infection in rice leaves induces phytocassanes and momilactones, both of which are major diterpenoid phytoalexins in rice, whereas CA mainly induces phytocassanes. We established a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry protocol for the rapid and accurate quantification of phytocassanes and momilactones. Using this method, we showed that CA preferentially induced the formation of phytocassanes in suspension-cultured rice cells, while a fungal chitin oligosaccharide elicitor induced that of both phytocassanes and momilactones. We further investigated the effects of CA on the expression of diterpene cyclase genes involved in phytoalexin biosynthesis. CA induced the transcription of the genes OsCPS2 (OsCyc2) and OsKSL7 (OsDTC1), which are involved in phytocassane biosynthesis, to a greater extent than the genes OsCPS4 (OsCyc1) and OsKSL4, which are involved in momilactone biosynthesis. OsCPS2 was particularly strongly induced, suggesting that it is one of the main mechanisms by which CA induces high levels of phytocassanes. (C) 2007 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
Phytochemistry, Feb. 2008, [Reviewed]

Defense-related signaling by interaction of arabinogalactan proteins and beta-glucosyl Yariv reagent inhibits gibberellin signaling in barley aleurone cells
Kiyoshi Mashiguchi; Eriko Urakami; Morifumi Hasegawa; Kazutsuka Sanmiya; Ichiro Matsumoto; Isomaro Yamaguchi; Tadao Asami; Yoshihito Suzuki, Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at the plasma membrane and in extracellular spaces. A synthetic chemical, beta-glucosyl Yariv reagent (beta-GlcY), binds specifically to AGPs. We previously reported that gibberellin signaling is specifically inhibited by beta-GlcY treatment in barley aleurone protoplasts. In the present study, we found that beta-GlcY also inhibited gibberellin-induced programmed cell death (PCD) in aleurone cells. We examined the universality and specificity of the inhibitory effect of beta-GlcY on gibberellin signaling using microarray analysis and found that beta-GlcY was largely effective in repressing gibberellin-induced gene expression. In addition, >100 genes were up-regulated by beta-GlcY in a gibberellin-independent manner, and many of these were categorized as defense-related genes. Defense signaling triggered by several defense system inducers such as jasmonic acid and a chitin elicitor could inhibit gibberellin-inducible events such as alpha-amylase secretion, PCD and expression of some gibberellin-inducible genes in aleurone cells. Furthermore, beta-GlcY repressed the gibberellin-inducible Ca(2+)-ATPase gene which is important for gibberellin-dependent gene expression, and induced known repressors of gibberellin signaling, two WRKY genes and a NAK kinase gene. These effects of beta-GlcY were also phenocopied by the chitin elicitor and/or jasmonic acid. These results indicate that gibberellin signaling is under the regulation of defense-related signaling in aleurone cells. It is also probable that AGPs are involved in the perception of stimuli causing defense responses., OXFORD UNIV PRESS
PLANT AND CELL PHYSIOLOGY, Feb. 2008, [Reviewed]

Isolation of octylphenol polyethoxylate-degrading soil bacteria: a long-term soil column study
Anne D. Rulianti; Morifumi Hasegawa; Yoko Ikunaga; Yoshinori Sato; Hiroyuki Ohta, A soil column was percolated with Triton X-100 (octylphenol polyethoxylates; OPEOn) under aerobic conditions for 300 days and OPEOn-degrading bacteria were isolated and characterized phylogenetically. Populations of total culturable bacteria and OPEOn (1000 mg L-1)-resistant bacteria increased 10 times and 46 times, respectively, during the percolation. 16S rRNA gene sequencing of 128 OPEOn-resistant isolates revealed an increase in the population level of Alphaproteobacteria and decreases in levels of Betaproteobacteria and Actinobacteria with the exposure to the compound. Four OPEOn-degrading strains were isolated and characterized as being related to Bradyrhizobium liaoningense (3 strains) and Afipia sp. (1 strain)., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
Microbes and Environments, 01 Dec. 2007, [Reviewed]

Identification of a biosynthetic gene cluster in rice for momilactones
Kazuhiro Shimura; Atsushi Okada; Kazunori Okada; Yusuke Jikumaru; Kwang-Wook Ko; Tomonobu Toyomasu; Takeshi Sassa; Morifumi Hasegawa; Osamu Kodama; Naoto Shibuya; Jinichiro Koga; Hideaki Nojiri; Hisakazu Yamane, Rice diterpenoid phytoalexins such as momilactones and phytocassanes are produced in suspension-cultured rice cells treated with a chitin oligosaccharide elicitor and in rice leaves irradiated with UV light. The common substrate geranylgeranyl diphosphate is converted into diterpene hydrocarbon precursors via a two-step sequential cyclization and then into the bioactive phytoalexins via several oxidation steps. It has been suggested that microsomal cytochrome P-450 monooxygenases (P-450s) are involved in the downstream oxidation of the diterpene hydrocarbons leading to the phytoalexins and that a dehydrogenase is involved in momilactone biosynthesis. However, none of the enzymes involved in the downstream oxidation of the diterpene hydrocarbons have been identified. In this study, we found that a putative dehydrogenase gene (AK103462) and two functionally unknown P-450 genes (CYP99A2 and CYP99A3) form a chitin oligosaccharide elicitor- and UV-inducible gene cluster, together with OsKS4 and OsCyc1, the diterpene cyclase genes involved in momilactone biosynthesis. Functional analysis by heterologous expression in Escherichia coli followed by enzyme assays demonstrated that the AK103462 protein catalyzes the conversion of 3 beta-hydroxy- 9 beta H-pimara-7,15-dien-19,6 beta-olide into momilactone A. The double knockdown of CYP99A2 and CYP99A3 specifically suppressed the elicitor-inducible production of momilactones, strongly suggesting that CYP99A2, CYP99A3, or both are involved in momilactone biosynthesis. These results provide strong evidence for the presence on chromosome 4 of a gene cluster involved in momilactone biosynthesis., AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2007, [Reviewed]

Disease resistance against Magnaporthe grisea is enhanced in transgenic rice with suppression of omega-3 fatty acid desaturases
Asanori Yara; Takashi Yaeno; Morifumi Hasegawa; Hideharu Seto; Jean-Luc Montillet; Kensuke Kusumi; Shigetni Seo; Koh Iba, Linolenic acid (18:3) is the most abundant fatty acid in plant membrane lipids and is a source for various oxidized metabolites, called oxylipins. 18:3 and oxylipins play important roles in the induction of defense responses to pathogen infection and wound stress in Arabidopsis. However, in rice, endogenous roles for 18:3 and oxylipins in disease resistance have not been confirmed. We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8. that synthesize 18:3. The F78Ri plants showed enhanced resistance to the phytopathogenic fungus Magnaporthe grisea. A typical 18:3-derived oxylipin, jasmonic acid (JA), acts as a signaling molecule in defense responses to fungal infection in Arabidopsis. However, in F78Ri plants, the expression of JA-responsive pathogenesis-related genes, PBZI and PR1b, was induced after inoculation with M. grisea, although the JA-mediated wound response was suppressed. Furthermore, the application of JA methyl ester had no significant effect on the enhanced resistance in F78Ri plants. Taken together, our results indicate that, although suppression of fatty acid desaturases involves the concerted action of varied oxylipins via diverse metabolic pathways, 18:3 or 18:3-derived oxylipins, except for JA, may contribute to signaling on defense responses of rice to M. grisea infection., OXFORD UNIV PRESS
PLANT AND CELL PHYSIOLOGY, Sep. 2007, [Reviewed]

酵母抽出液（アグリボEX）処理によるPR遺伝子群発現誘導機構の解析
小原直美; 光原一朗; 瀬尾茂美; 大橋祐子; 長谷川守文; 松浦雄介, Application of AGREVO EX, a plant energizer made of yeast extract (YE), promotes root growth and suppresses plant diseases. Because no antimicrobial activity was found in the YE itself, YE induction of the plant's self-defense system has been suggested. To study the mechanism of enhanced disease resistance, the effect of YE on the expression of defense-related genes was analyzed in detached tobacco leaves. YE induced the expression of basic PR-1, -2, and -6 genes but not the acidic PR-1 gene. The YE solution itself produced ethylene, and ethylene emission from tobacco leaf discs was enhanced by the YE treatment. Also YE induced accumulation of PR-1, -2, and -3 proteins, and its suppression by treatment with silver thiosulfate (STS), which inhibits the perception of ethylene, suggesting the involvement of ethylene in YE-induced PR protein accumulation. The YE treatment did not induce resistance to Tobacco mosaic virus (TMV), but likely induced resistance to Ralstonia solanacearum and Rhizoctonia solani in tobacco plants, suggesting that YE may enhance resistance against necrotrophic pathogens., The Phytopathological Society of Japan (PSJ)
日本植物病理学会報, May 2007, [Reviewed]

Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice
Masaki Mori; Chikako Tomita; Kazuhiko Sugimoto; Morifumi Hasegawa; Nagao Hayashi; Joseph G. Dubouzet; Hirokazu Ochiai; Hitoshi Sekimoto; Hirohiko Hirochika; Shoshi Kikuchi, A lesion mimic mutant that we designated Spotted leaf 18 (Spl18) was isolated from 13,000 activation-tagging lines of rice produced by our modified activation-tagging vector and further characterized. Spl18 was dominant and its phenotype was linked to the T-DNA insertion. An ORF was located about 500 bp downstream of the inserted T-DNA, and the deduced protein, designated OsAT1, showed sequence similarity to an acyltransferase whose expression is induced by hypersensitive reaction in tobacco. The transcriptional level of OsAT1 was very low in the WT leaf blade but high in Spl18 leaf blade. In wild-type rice, OsAT1 was transcribed mainly in the young panicle, in the panicle just after heading, and in the leaf sheath. In addition, transcription of the genes for PR protein was upregulated in Spl18, accumulation of phytoalexins (both momilactone A and sakuranetin) was increased, and resistance to blast disease was improved. We then combined OsAT1 genomic DNA downstream of the modified 35S promoter and re-transformed it into rice. Lesion mimic and blast resistance phenotypes were detected in the transgenic lines produced, clearly indicating that overexpression of OsAT1 caused the Spl18 phenotypes. In addition, plants overexpressing OsAT1 showed resistance to bacterial blight., SPRINGER
PLANT MOLECULAR BIOLOGY, Apr. 2007, [Reviewed]

Sequential regulation of gibberellin, brassinosteroid, and jasmonic acid biosynthesis occurs in rice coleoptiles to control the transcript levels of anti-microbial Thionin genes
Yukihiro Kitanaga; Cui Jian; Morifumi Hasegawa; Junshi Yazaki; Naoki Kishimoto; Shoshi Kikuchi; Hidemitsu Nakamura; Hiroaki Ichikawa; Tadao Asami; Shigeo Yoshida; Isomaro Yamaguchi; Yoshihito Suzuki, Transcripts of thionin genes encoding antimicrobial peptides were present at a high level in rice coleoptiles just after germination, and decreased to an undetectable level after about 3 d, but this decline was suppressed by co-treatment with gibberellic acid (GA3) and brassinolide (BL). The temporal expression patterns of key enzyme genes for the biosyntheses of gibberellins (GAs) and brassinosteroids (BRs) were correlated with the fluctuation of thionin mRNAs. Jasmonic acid (JA) replaced the effect of GA3 and BL, and its change in endogenous level was parallel to that of the thionin genes. These results strongly suggest that thionin gene expression was positively regulated by JA, whose endogenous level was synergistically regulated by GAs and BRs. In contrast, thionin gene expression in etiolated seedlings remained high while the endogenous level of JA was low, suggesting the presence of another signaling pathway in the dark to maintain the thionin level., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Oct. 2006, [Reviewed]

Cloning and functional analysis of caffeic acid 3-O-methyltransferase from rice (Oryza sativa)
Fengqiu Lin; Go Yamano; Morifumi Hasegawa; Hiroyuki Anzai; Shinji Kawasaki; Osamu Kodama, Corresponding, A putative O-methyltransferase cDNA was cloned from UV-irradiated rice leaves based on an amino acid sequence reported as that of naringenin 7-O-methyltransferase, which is involved in the biosynthesis of a rice phytoalexin, sakuranetin. However, the recombinant protein (approximately 41 kDa) expressed in Escherichia coli showed not naringenin 7-O-methyltransferase activity but caffeic acid 3-O-methyltransferase activity. Semi-quantitative RTPCR revealed that the mRNA was expressed in all tissues tested and was not affected by CuCl2, jasmonic acid or UV treatment. The enzyme also methylated flavonoids which have two adjacent hydroxyl groups in the B ring. (c) Pesticide Science Society of Japan., PESTICIDE SCI SOC JAPAN
Journal of Pesticide Science, 21 Feb. 2006, [Reviewed]

Similarities and differences between the characteristics of gibberellin-binding protein and gibberellin 2-oxidases in adzuki bean (Vigna angularis) seedlings
SH Park; M Nakajima; M Hasegawa; Yamaguchi, I, Gibberellin-binding proteins (GBPs) were purified ca. 230,000 fold. The characteristics of adzuki GBP were examined and compared with those of a recombinant gibberellin 2-oxidase (rVaGA2oxA1) that was fused with glutathione S-transferase (GST). VaGA2oxA1 was most abundantly expressed in etiolated adzuki bean seedlings, and VaGA2oxA1 and GBPs from adzuki bean seedlings showed gibberellin-binding activity when incubated with 2-oxoglutarate and Co2+. Both rVaGA2oxA1 and partially purified GBPs from adzuki bean seedlings showed very similar selectivity to gibberellins in binding assays, where biologically active gibberellins such as GA(4), GA(3), GA(7), and GA(1) showed higher binding affinity than biologically inactive gibberellins such as GA(8), GA(34), and 3-epi-GA(4). The polyclonal antibody raised against rVaGA2oxA1 cross-reacted with all rVaGA2oxs (rVaGA2oxA1, rVaGA2oxA2, rVaGA2oxB1, rVaGA2oxB2, and rVaGA2oxB3) whose cDNAs were cloned from adzuki bean seedlings. Treated with the antibody, the recombinants that originally showed gibberellin-binding activity lost both binding activity and enzymatic activity. In contrast to the recombinants, the gibberellin-binding activity of GBPs from adzuki bean seedlings was hardly affected by the antibody treatment. The GBPs showed very weak gibberellin 2-oxidase-like activity, and it was not affected by the antibody treatment either. These observations suggest that a major component that showed GA-binding activity was apparently different from any gibberellin 2-oxidase cloned from the seedlings., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, 23 Aug. 2005

Volatile emission by [N-(-)-jasmonoyl]-alanylleucine from rice leaves (Oryza sativa L.)
S Tamogami; M Suzuki; H Toshima; M Hasegawa; O Kodama; M Noma, A dipeptide conjugate of jasmonic acid, [N-(-)-jasmonoyl]-L-alanyl-L-leucine, was prepared and its biological activities were investigated. The conjugate was active in the emission of linalool from rice leaves, while [N-(-)-jasmonoyl]-D-alanyl-L-leucine was not. Neither conjugate was active in the production of sakuranetin. This is the first report of a biologically active dipeptide conjugate of jasmonic acid. (c) Pesticide Science Society of Japan., PESTICIDE SCI SOC JAPAN
Journal of Pesticide Science, 20 Aug. 2005

Rice fatty acid alpha-dioxygenase is induced by pathogen attack and heavy metal stress: activation through jasmonate signaling
T Koeduka; K Matsui; M Hasegawa; Y Akakabe; T Kajiwara, Plant fatty acid alpha-dioxygenases (DOXs) catalyze the stereospecific conversion of fatty acids into the corresponding (R)-2-hydroperoxy fatty acids. In several plant species the corresponding gene was shown to be induced by pathogen infection, herbivore attack and environmental stresses. The precise signaling pathway accountable for the induction remains unidentified. In the present study, the effects of bacterial infection, oxidative- and heavy metal-stresses, and plant signaling molecules such as jasmonate, salicylic acid (SA), and ethylene (ET) on expression of a fatty acid alpha-DOX (OsDOX) gene in rice seedlings were investigated. The rice blight bacteria, Xanthomonas oryzae, elicited the accumulation of OsDOX transcripts in the leaves in both the incompatible and compatible interactions. Treating the seedling with CuSO4 also significantly enhanced the OsDOX expression. The degree of induction was shown to be mostly parallel to the level of endogenous jasmonic acid (JA) in the leaves. In contrast, SA was little effective and ET down-regulated not only the OsDOX expression but also the endogenous level of JA in rice seedlings. These results suggested that the OsDOX gene expression by a variety of stress-related stimuli was activated through jasmonate signaling and was negatively regulated by ET (c) 2005 Elsevier GmbH. All rights reserved., URBAN & FISCHER VERLAG
JOURNAL OF PLANT PHYSIOLOGY, Aug. 2005

Involvement of the basic helix-loop-helix transcription factor RERJ1 in wounding and drought stress responses in rice plants
Kyoko Kiribuchi; Yusuke Jikumaru; Hanae Kaku; Eiichi Minami; Morifumi Hasegawa; Osamu Kodama; Hideharu Seto; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane, The jasmonic acid (JA)-responsive gene RERJ1 isolated from suspension-cultured rice cells encodes a transcription factor with a basic helix-loop-helix motif. In this study, we found that RERJ1 is also expressed in rice plants in response to JA, and that its expression in rice leaves is up-regulated by exposure to wounding and drought stress. It is also suggested that JA but not abscisic acid is involved in the up-regulation of RERJ1 expression caused by wounding and drought stress., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, 24 May 2005

Degradation profiles of branched nonylphenol isomers by Sphingobium amiense and Sphingomonas cloacae
Yoko Ikunaga; Shu-ichi Miyakawa; Morifumi Hasegawa; Yasuhiro Kasahara; Osamu Kodama; Hiroyuki Ohta
Soil Science and Plant Nutrition, Nov. 2004

Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins
Yusuke Jikumaru; Tadao Asami; Hideharu Seto; Shigeo Yoshida; Tadashi Yokoyama; Naomi Obara; Morifumi Hasegawa; Osamu Kodama; Makoto Nishiyama; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane, Several types of jasomonic acid (JA) derivatives, including JA-amino acid conjugates, a JA-biotin conjugate, a JA-dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA-binding proteins. These JA derivatives, excepting the JA-fluoresceine conjugate, exhibited significant biological activities in a rice seedling assay, a rice phytoalexin-inducing assay, and/or a soybean phenylalanine ammonia-lyase-inducing assay. These A derivatives could therefore be useful probes for identifying JA-binding proteins. The activity spectra of the prepared compounds were different from each other, suggesting that different types of JA receptors were involved in the perception of A derivatives in the respective bioassays., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Jul. 2004

Dihydrocoronatine, a promising candidate of chemical probe studying, coronatine-, jasmonoid- and octadecanoid-binding protein
Munenori Suzuki; Morifumi Hasegawa; Osamu Kodama; Hiroaki Toshima, Coronatine (1), its synthetic analogs (6-13) and jasmonic acid induced various volatiles in rice leaves. In the range of 0.01-0.1 mm, dihydrocoronatine (7) exhibited 4-687 times higher activity for linalool emission than that of 1. The radioactive derivative of 7, [4,5-H-3]-7, was employed to identify the putative coronatine-binding protein in rice leaves. 7 would be a promising candidate for a chemical probe to study cornatine-binding protein related to the jasmonoid and octadecanoid signaling pathway in higher plants. A detailed study of coronatine-binding protein in rice leaves and cell culture with [4,5-H-3]-7 is now in progress., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Jul. 2004, [Reviewed]

Enhanced resistance to blast fungus and bacterial blight in transgenic rice constitutively expressing OsSBP, a rice homologue of mammalian selenium-binding proteins
Kazutoshi Sawada; Morifumi Hasegawa; Lena Tokuda; Junko Kameyama; Osamu Kodama; Takayuki Kohchi; Kazuya Yoshida; Atsuhiko Shinmyo, The rice Oryza sativa selenium-binding protein homologue (OsSBP) gene encodes a homologue of mammalian selenium-binding proteins, and it has been isolated as one of the genes induced by treating a plant with a cerebroside elicitor from rice blast fungus. The possible role of OsSBP in plant defense was evaluated by using a transgenic approach. Plants overexpressing OsSBP showed enhanced resistance to a virulent strain of rice blast fungus as well as to rice bacterial blight. The expression of defense-related genes and the accumulation of phytoalexin after infection by rice blast fungus were accelerated in the OsSBP overexpressors. A higher level of H2O2 accumulation and reduced activity of such scavenging enzymes as ascorbate peroxidase and catalase were seen when the OsSBP-overexpressing plants were treated with the protein phosphatase 1 inhibitor, calyculin A. These results suggest that the upregulation of OsSBP expression conferred enhanced tolerance to different pathogens, possibly by increasing plant sensitivity to endogenous defense responses. Additionally, the OsSBP protein might have a role in modulating the defense mechanism to biotic stress in rice., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Apr. 2004

Purification and identification of antimicrobial sesquiterpene lactones from yacon (Smallanthus sonchifolius) leaves
Fengqiu Lin; Morifumi Hasegawa; Osamu Kodama, Corresponding, The extraction of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson; Asteraceae] leaves and chromatographic separation yielded two new antibacterial melampolide-type sesquiterpene lactones, 8beta-tigloyloxymelampolid-14-oic acid methyl ester and 8beta-methacryloyloxymelampolid-14-oic acid methyl ester, as well as the four known melampolides, sonchifolin, uvedalin, enhydrin and fluctuanin. The newly identified compound, 8beta-methacryloyloxymelampolid-14-oic acid methyl ester, exhibited potent antimicrobial activity against Bacillus subtilis and Pyricularia oryzae, while 8beta-tigloyloxymelampolid-14-oic acid methyl ester showed lower activity. Fluctuanin exhibited the strongest antibacterial activity against B. subtilis among these six sesquiterpene lactones., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Oct. 2003, [Reviewed]

Induced volatiles in elicitor-treated and rice blast fungus-inoculated rice leaves
Naomi Obara; Morifumi Hasegawa; Osamu Kodama, Corresponding, The volatiles released from elicitor (copper chloride, jasmonic acid, UV, L-methionine and chitosan oligomer)-treated and rice blast fungus-inoculated rice leaves were collected by the solid-phase microextraction technique and analyzed by GC-MS. (Z)-3-Hexen-1-ol, monoterpenes, methyl salicylate, and sesquiterpenes were identified as elicitor-induced volatiles by a comparison of their GC retention times and mass spectra with those of authentic compounds. The different elicitors resulted in some qualitative and quantitative differences in the production of volatiles. Monoterpenes and sesquiterpenes were identified as the rice blast fungus-induced volatiles., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Dec. 2002, [Reviewed]

Synthesis of (17R)- and (17S)-isomers of volicitin, an elicitor of plant volatiles contained in the oral secretion of the beet armyworm
Seiji Itoh; Shigefumi Kuwahara; Morifumi Hasegawa; Osamu Kodama, Both the (17R)- and (17S)-isomers of volicitin, which is contained in the oral secretion of the beet armyworm and induces corn seedlings to emit a blend of volatile compounds to attract the natural enemy of the herbivore, were synthesized via the semi-hydrogenation of an intermediary diyne and (Z)-selective olefination as the key steps. They were both obtained as crystalline compounds., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Jul. 2002

Biosynthesis of rice phytoalexin: enzymatic conversion of 3b-hydroxy-9b-pimara-7,15-dien-19,6b-olide to momilactone A
Anotai Atawong; Morifumi Hasegawa; Osamu Kodama, Corresponding, Momilactone A, a major rice diterpene phytoalexin, could be synthesized by dehydrogenation at the 3-position of 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide in rice leaves. The presence of 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide in UV-irradiated rice leaves was confirmed by comparing the mass spectra and retention times after a GC/MS analysis of the natural and synthetic compounds. The soluble protein fraction from UV-irradiated rice leaves showed dehydrogenase activity to convert 3beta-hydroxy-9beta-pimara-7,15-dien-19,6betaolide into momilactone A. The enzyme required NAD(+) or NADP(+) as a hydrogen acceptor. The optimum pH for the reaction was 8. The K-m value to 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide was 36 mum when NAD(+) was supplied as a cofactor at a concentration of 1 mm. 3beta-Hydroxy-9beta-pimara-7,15-dien-19,6beta-olide and its dehydrogenase activity were induced in a time-dependent manner by UV irradiation., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Mar. 2002, [Reviewed]

Essential role of the small GTPase Rac in disease resistance of rice
Eiichiro Ono; Hann Ling Wong; Tsutomu Kawasaki; Morifumi Hasegawa; Osamu Kodama; Ko Shimamoto
Proceedings of the National Academy of Sciences of the United States of America, Feb. 2001

Organic Nutrient-dependent Degradation of Branched Nonylphenol by Sphingomonas sp. YT Isolated from a River Sediment Sample
Ynte P. De Vries; Yoshiharu Takahara; Yoko Ikunaga; Yuuji Ushiba; Morifumi Hasegawa; Yasuhiro Kasahara; Hirofumi Shimomura; Shunji Hayashi; Yoshikazu Hirai, A conventional enrichment culture on branched nonylphenol (NP) with diluted nutrient broth as an additional source of organic nutrients yielded a bacterial strain able to degrade branched NP. The isolate (designated YT) was identified as Sphingomonas sp. based on an analysis of its 16S ribosomal RNA genes and cellular lipids. The degradation of NP by strain YT occurred primarily during the exponential phase of cell growth in cultures on a yeast extract-mineral salts medium. The degree of degradation was directly proportional to the amount of yeast extract present in the medium and no significant growth occurred when NP was the sole source of carbon and energy. Gas chromatography-mass spectrometry (GC-MS) of resting cell suspensions incubated with branched NP revealed that the degradation did not yield any metabolites containing aromatic residues but only branched alcohols. When a linear NP was used as the target substrate, GS-MS of the suspensions indicated the appearance of a hydroxylated linear NP as an intermediate during the degradation. Strain YT is expected to attack NP by an initial oxidative cleavage of the phenol ring. © 2001, Japanese Society of Microbial Ecology &
The Japanese Society of Soil Microbiology. All rights reserved.
Microbes and Environments, 2001

Induced accumulation of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDIMBOA-Glc) in maize leaves
Akira Oikawa; Atsushi Ishihara; Morifumi Hasegawa; Osamu Kodama; Hajime Iwamura, Accumulation of 2-(2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one)-beta -D-glucopyranose (HDM BOA-Glc) was induced in maize leaves by treatment with CuCl(2), chitopentaose, penta-N-acetylchitopentaose, or jasmonic acid (JA). The accumulation of HDMBOA-Glc was accompanied by a decrease in level of 2-(2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one)-beta -D-glucopyranose (DIMBOA-Glc). When the leaf segments were treated with JA in the presence of [Me-(2)H(3)]L-methionine, the label was efficiently incorporated into HDMBOA-Glc, while no incorporation into DIMBOA-Glc or HMBOA-Glc was detected, suggesting the conversion of constitutive DIMBOA-Glc to HDMBOA-Glc by methylation at the 4-position. Levels of endogenous 5.4 and its leucine conjugate transiently increased prior to the accumulation of HDMBOA-Glc in leaf segments treated with CuCl(2) and chitopentaose. The lipoxygenase inhibitor ibuprofen suppressed the accumulation of HDMBOA-Glc induced by CuCl(2) treatment, and the reduced accumulation of HDMBOA-Glc was recovered by addition of JA. These findings suggested that JA functions as a signal transducer in the induction of HDMBOA-Glc accumulation. (C) 2001 Elsevier Science Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
Phytochemistry, 2001

Simple synthesis of 5,9-dimethylated long-chain alkanes, the sex pheromones of leaf miner moths
T Liang; S Kuwahara; M Hasegawa; O Kodama, Each stereoisomeric mixture of 5,9-dimethylpentadecane and 5,9-dimethylhexadecane, the major and the minor sex pheromone components of Perileucoptera coffeella, respectively, was synthesized in about 25% overall yield through 6 steps from beta -citronellol, 5,9-Dimethylheptadecane, the major sex pheromone component of Leucoptera malifoliella, was also synthesized analogously as a stereoisomeric mixture in a 22% overall yield., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Nov. 2000

Methionine-induced phytoalexin production in rice leaves
Yumiko Nakazato; Shigeru Tamogami; Hiroshi Kawai; Morifumi Hasegawa; Osamu Kodama, The application of methionine on wounded rice leaves induced the production of rice phytoalexins, sakuranetin and momilactone A. This induction resulted from stimulation of phenylalanine ammonia-lyase and naringenin 7-O-methyltransferase activity. Jasmonic acid, ethylene, and active oxygen species are important as signal transducers in disease resistance mechanisms. However, although the endogenous level of jasmonic acid rapidly increased in reaction to wound, methionine treatment could not induced endogenous JA production. Ethylene induced the production of the flavonoid phytoalexin, sakuranetin, but did not induce the production of :a terpenoid phytoalexin, momilactone A. On the other hand, a free radical scavenger, Tiron, counteracted the induction of both sakuranetin and momilactone A production in methionine-treated leaves. Active oxygen species may be important in methionine-induced production of phytoalexins., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, Mar. 2000, [Reviewed], [Invited]

Semi quantification of gibberellins in the anthers of thermosensitive genetic male sterile rice (Oryza sativa L. cv. PL12)
Ichiro Honda; Shinya Iwasaki; Kazuhisa Sudo; Hiroshi Kato; Kiyoaki Maruyama; Morifumi Hasegawa; Isomaro Yamaguchi; Noboru Murofushi; Tadashi Yanagisawa; Nobutaka Takahashi, The levels of endogenous GAs in the anthers of rice (Oryza sativa L., cv. Reimei (normal)) and in those of sterile and fertile plants of a thermosensitive genetic mate sterile line (Norin PL12; derived from Reimei) were measured by enzyme-linked immunosorbent assay (ELISA). High levels of GA(4/7) were detected in the anthers of Reimei (normal fertility) and fertile (growing under fertile conditions) PL12, while such levels were markedly reduced with increased sterility in PL12, The anthers of the plants exposed to the sterile conditions contained undeveloped pollens, These results suggest that the occurrence of GA(4/7) was closely related to the expression of the sterility gene and the growth and/or development of the anther and/or pollen PL12., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, 1997

Partial purification and characterization of a gibberellin-binding protein from seedlings of Azukia angularis
Masatoshi Nakajima; Keiji Takita; Hideki Wada; Kenji Mihara; Morifumi Hasegawa; Isomaro Yamaguchi; Noboru Murofushi, Little is known about mechanisms for the perception of gibberellin (GA), a phytohormone, by plants. A GA-binding protein (GBP) with receptor-like characteristics was partially purified from azuki bean (Azukia angularis) seedlings. Gibberellins that caused much elongation of azuki epicotyl sections showed strong binding affinity for the GBP, and their biosynthetic precursors and inactive metabolites had weak affinity for it. The apparent K-d value for GA(4) was calculated to be 7 x 10(-10) M from Scatchard plots. The molecular weight of the GBP in the native state was estimated to be 25,000 by high-performance liquid chromatography with a gel-permeation column. The GBP may be one of the receptor candidates for GAs. (C) 1997 Academic Press., ACADEMIC PRESS INC
Biochemical and Biophysical Research Communications, 1997

A methyltransferase for synthesis of the flavanone phytoalexin sakuranetin in rice leaves
Randeep Rakwal; Morifumi Hasegawa; Osamu Kodama, Rice (Oryza sativa L.) leaves irradiated with short wave UV light accumulated the major rice phytoalexin, flavanone sakuranetin. The extracts from these leaves catalyzed the methylation of the hydroxyl group at position 7 of naringenin to yield sakuranetin, with S-adenosyl-L-methionine as the methyl donor. Activity of the naringenin 7-O-methyltransferase was nor found in healthy rice leaves but increased upon irradiation with UV light in parallel with increase in sakuranetin. The autoradiogram of the enzymatic product on 2D-TLC was found to be sakuranetin. The enzyme did not methylate sakuranetin, but methylated other flavanones, but not isoflavanones. (C) 1996 Academic Press. Inc., ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Biochemical and Biophysical Research Communications, 1996, [Reviewed]

Endogeneous levels of gibberellins in normal and male sterile anthers of rice (Oryza sativa cv. Nihonmasari)
Morifumi Hasegawa; Masatoshi Nakajima; Kazuyoshi Takeda; Isomaro Yamaguchi; Noboru Murofushi, Lead, Endogenous gibberellins in anthers of rice (Oryza sativa, cv, Nihonmasari (normal)) and in male sterile lines derived from Nihonmasari were quantitated by an immunoassay, The content of GA(4/7) and the dry weight of the anthers in the normal rice increased rapidly 1-2 days before heading. The content of gibberellins in the male sterile rice were roughly correlated with the pollen developmental stage characteristic of each line., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, 1995, [Reviewed], [Invited]

Immunohistochemistry of gibberellins in rice anthers
Morifumi Hasegawa; Nobuyoshi Hashimoto; Jun Zhang; Masatoshi Nakajima; Kazuyoshi Takeda; Isomaro Yamaguchi; Noboru Murofushi, Lead, A new immunohistochemical method for analyzing the localization of gibberellins was developed and applied to rice anthers. This method uses 1,3-diisopropylcarbodiimide gas fixation to prevent the drift of gibberellins during the fixation procedure, Spikelets before anthesis were frozen in liquid nitrogen and lyophilized, They were then fixed with diisopropylcarbodiimide gas in a desiccator, which catalyzed amide formation between the 7-carboxyl groups of gibberellins and the amino groups of proteins in the tissue, The fixed spikelets were embedded in paraffin and sectioned, the sections being stained with the anti-GA(1)-Me antibody and visualized by using an immunoperoxidase system, The anthers just before anthesis were stained well, while those 4-5 days before heading were not stained, We analyzed the gibberellins in an extract of the rice anthers by an immunoassay, using the anti-GA(1)-Me antibody, On the basis of the results, we conclude that the major substances stained in this immunohistochemical study should be 16 alpha,17-dihydroxy-16,17-dihydrogibberellin A(4)-17-O-beta-D-glucoside and 16 alpha,17-dihydroxy-16,17-dihydrogibberell A(7)-17-O-beta-D-glucoside., TAYLOR & FRANCIS LTD
Bioscience, Biotechnology, and Biochemistry, 1995, [Reviewed]

A novel gibberellin glucoside, 16a, 17-dihydroxy-16,17-dihydrogibberellin A₄-17-b-D-glucoside, from rice anthers
Morifumi Hasegawa; Masatoshi Nakajima; Kazuyoshi Takeda; Isomaro Yamaguchi; Noboru Murofushi, Lead, A novel gibberellin glucoside, which was found as an immunoreactive substance against anti-GA(4)-antibody in extracts of rice anthers, has been identified as 16 alpha,17-dihydroxy-16,17-dihydrogibberellin A(4)-17-O-beta-D-glucopyranoside by comparison of the mass spectra and Kovats retention indices after GC-MS of permethylated derivatives of the natural and synthetic compound. Co-occurrence of 16 alpha,17-dihydroxy-16,17-dihydrogibberellin A(7)-17-O-beta-D-glucoside was also indicated., PERGAMON-ELSEVIER SCIENCE LTD
Phytochemistry, 1994, [Reviewed], [Invited]

植物の自己防御物質フィトアレキシンの多様性
長谷川守文
化学と生物, 20 Jul. 2017, [Reviewed], [Invited]

シアナイドはジテルペノイド系ファイトアレキシン誘導を介していもち病抵抗性に寄与する
光原一朗; 瀬尾茂美; 長谷川守文; 井上靖乃; 岡田憲典; 山根久和; 大橋祐子
日本植物病理学会大会プログラム・講演要旨予稿集, 21 Mar. 2013

The potential bioproduction of the pharmaceutical agent sakuranetin, a flavonoid phytoalexin in rice
Takafumi Shimizu; Fengqiu Lin; Morifumi Hasegawa; Hideaki Nojiri; Hisakazu Yamane; Kazunori Okada
BIOENGINEERED, Nov. 2012, [Reviewed], [Invited]

シアナイドはモミラクトンAまたはサクラネチンと協調的にイネのいもち病抵抗性に寄与する
瀬尾茂美; 光原一朗; 長谷川守文; 岡田憲典; 山根久和; 大橋祐子
日本植物病理学会大会プログラム・講演要旨予稿集, 15 Mar. 2012

フラボノイド系ファイトアレキシン・サクラネチンがイネのいもち病抵抗性に果たす役割
光原一朗; 瀬尾茂美; 長谷川守文; 岡田憲典; 山根久和; 大橋裕子
日本植物病理学会大会プログラム・講演要旨予稿集, 15 Mar. 2012

HRにおいてエチレンと共に生成するシアナイドはイネのいもち病抵抗性に寄与する
瀬尾茂美; 光原一朗; JIAO F; 岩井孝尚; 長谷川守文; 大橋祐子
日本植物病理学会大会プログラム・講演要旨予稿集, 11 Mar. 2011

64. Identification of a metabolite of rice phytoalexin momilactone A by rice blast fungus
Imai Takuya; Ohashi Yuko; Mitsuhara Ichiro; Seo Shigemi; Toshima Hiroaki; Hasegawa Morifumi
植物化学調節学会研究発表記録集, 01 Oct. 2010

イネフィトアレキシンモミラクトンAのいもち病菌による代謝産物の同定
今井卓也; 大橋祐子; 光原一朗; 瀬尾茂美; 戸嶋浩明; 長谷川守文
植物の生長調節, 01 Oct. 2010

イネフィトアレキシン モミラクトンAのイネいもち病菌による代謝
今井卓也; 大橋祐子; 光原一朗; 瀬尾茂美; 戸嶋浩明; 長谷川守文
日本農芸化学会大会講演要旨集, 05 Mar. 2010

いもち病菌に感染した抵抗性および罹病性イネでのファイトアレキシンの蓄積とその分解
長谷川守文; 光原一朗; 瀬尾茂美; 古賀仁一郎; 岡田憲典; 山根久和; 大橋祐子
日本植物病理学会報, 25 Aug. 2009

ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究
増口潔; 浦上恵理子; 長谷川守文; 三宮一宰; 松本一朗; 山口五十麿; 浅見忠男; 鈴木義人
日本農薬学会大会講演要旨集, 27 Feb. 2009

Disease Resistance against Magnaporthe grisea in Transgenic Rice Lines with Suppression of Allene Oxide Cyclase Gene or 12-oxo-Phytodienoic Acid Reductase Genes
Yara Asanori; Yaeno Takashi; Hasegawa Morifumi; Kusumi Kensuke; Seo Shigemi; Iba Koh
Plant and Cell Physiology Supplement, 2008

Enhanced Resistance against Magnaporthe grisea in Transgenic Rice with Suppression of ω-3 Fatty Acid Desaturase Genes
Yara Asanori; Yaeno Takashi; Jean-Luc Montillet; Hasegawa Morifumi; Kusimi Kensuke; Seo Shigemi; Iba Koh
Plant and Cell Physiology Supplement, 2007

Induced disease resistance in rice plants infested with whitebacked planthopper, Sogatella furcifera　　　　　　　　　　　　　　　
Masaru Satoh; Kenji Gomi; Hiroo Kanno; Morifumi Hasegawa; Osamu Kodama; Yuko Ohashi; Junji Takabayashi
The 4th Asia-Pacific Conference on Chemical Ecology, 2007

Disease resistance against Magnaporthe grisea in transgenic rice with suppression of plastid ω-3 fatty acid desaturase genes
Yara Asanori; Hattori Yasuyuki; Yaeno Takashi; Hasegawa Morifumi; Seo Shigemi; Kusumi Kensuke; Iba Koh
Plant and Cell Physiology Supplement, 2006

Construction of transgenic rice with reduced level of 12-oxo-phytodienoic acid
Hattori Yasuyuki; Yara Asanori; Yaeno Takashi; Hasegawa Morihumi; Kusumi Kensuke; Iba Koh
Plant and Cell Physiology Supplement, 2006

(266) Analysis on the Induced Resistance to Pathogens in Rice plants Infested by Sogatella furcifera(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)
Satoh M.; Kanno H.; Mitsuhara I.; Ohashi Y.
Annals of the Phytopathological Society of Japan, 25 Aug. 2005

Construction of transgenic rice plants in which the expression of jasmonic acid biosynthesis genes is suppressed by RNA interference method
Hattori Yasuyuki; Yara Asanori; Yaeno Takashi; Hasegawa Morifumi; Kusumi Kensuke; Iba koh
Plant and Cell Physiology Supplement, 2005

Characterization of jasmonic acid-reduced transgenic rice
Yara Asanori; Hattori Yasuyuki; Yaeno Takashi; Hasegawa Morifumi; Seo Shigemi; Kusumi Kensuke; Iba koh
Plant and Cell Physiology Supplement, 2005

ノニルフェノール類化学物質による環境汚染と分解微生物：土壌における実態の解明に向けて
太田寛行; 高原義治; 生長陽子; 牛場裕司; 宮川修一; 長谷川守文; 笠原康裕; 久留主泰朗; 小野勝道; 久保田俊夫
日本微生物生態学会誌, 2002

Purification of gibberellin-binding protein in etiolated azuki bean seedlings
IDA Kentaro; NAKAJIMA Masatoshi; Xu Zehng-Jun; HASEGAWA Morifumi; PARK Seung-Hyun; TOMIOKA Katsushi; MUROFUSHI Noboru; YAMAGUCHI Isomaro
Chemical Regulation of Plants, 2000

D-42 Purification of gibberellin-binding protein in etiolated azuki bean seedlings
MIHARA Kenji; NAKAYAMA Akira; HASEGAWA Morifumi; NAKAJIMA Masatoshi; MUROFUSHI Noboru; YAMAGUCHI Isomaro
The Janapese Society for Chemical Regulation of Plants, Abstract, 1998

D-41 Immunohistochemistry of gibberellins in developing seeds of Pharbitis nil.
NAKAJIMA Masatoshi; PARK Seijin; HASEGAWA Morifumi; MUROFUSHI Noboru; YAMAGUCHI Isomaro
The Janapese Society for Chemical Regulation of Plants, Abstract, 1998

4 Purification of Gibberellin-binding Protein in Etiolated Azuki Bean
Wada Hideki; Mihara Kenji; Takita Keiji; Hasegawa Morifumi; Nakajima Masatoshi; Yamaguchi Isomaro; Murohushi Noboru
The Janapese Society for Chemical Regulation of Plants, Abstract, 1996

14 Immunohistochemical Analysis of Gibberellins in Immature Seed of Pharbitis nil
Park Seijin; Hasegawa Morifumi; Nakajima Masatoshi; Yamaguchi Isomaro; Murofushi Noboru
The Janapese Society for Chemical Regulation of Plants, Abstract, 1996

A-08 イネ葯におけるジベレリンの免疫組織化学
長谷川 守文; 橋本 展誉; 張 軍; 中嶋 正敏; 武田 和義; 山口 五十麿; 室伏 旭
植物化学調節学会 研究発表記録集, 1995

4 イネ葯中に含まれる新規ジベレリン配糖体について
長谷川 守文; 中嶋 正敏; 山口 五十麿; 室伏 旭
植物化学調節学会 研究発表記録集, 1993

植物ホルモンと細胞の形　　　　　　　　　　　　　　　
山口五十麿; 中嶋正敏; 長谷川守文, Joint work
学会出版センター, Feb. 1998
4762218707

レンコン粉末の麹化によるヌシフェリン産生機構の解明　　　　　　　　　　　　　　　
柳橋諒; 藤井智教; 大西賢一; 長谷川守文; 豊田淳; 望月佑哉; 井上栄一
園芸学会令和 5 年度春季大会, 20 Mar. 2023, 園芸学会
20230315, 20230322

ホウレンソウ葉のフラボン型ファイトアレキシン　　　　　　　　　　　　　　　
村田悠夏; 鳥井潤一郎; 戸嶋浩明; 長谷川守文
日本農芸化学会 2023 年度大会, 15 Mar. 2023, 日本農芸化学会
20230314, 20230317

糸状性シアノバクテリア由来フィコシアノビリンの生産開発と特性解析　　　　　　　　　　　　　　　
⻘木仁一; 鎗田孝; ⻑谷川守文; 朝山宗彦
第 45 回日本分子生物学会年会, 02 Dec. 2022, 日本分子生物学会
20211130, 20221202

麹化によるレンコン粉末の機能性成分量の改変　　　　　　　　　　　　　　　
柳橋諒; 藤井智教; 大西賢一; 長谷川守文; 豊田淳; 井上栄一
園芸学会令和 3 年度秋季大会, 11 Sep. 2021, 園芸学会
20210908, 20210914

ホウレンソウ葉における塩化銅ストレス誘導性化合物の同定　　　　　　　　　　　　　　　
鳥井潤一郎; 戸嶋浩明; 長谷川守文
日本農芸化学会 2021 年度大会, 19 Mar. 2021, 日本農芸化学会
20210318, 20210321

ジャスモン酸メチルによってイネ根部で誘導されるジテルペノイドファイトアレキシン類の探索　　　　　　　　　　　　　　　
早川優介; 手塚大介; 菊地爽太; 今井亮三; 豊増知伸; 戸嶋浩明; 長谷川守文
日本農薬学会第 46 回大会, 09 Mar. 2021, 日本農薬学会
20210308, 20210310

Detoxification metabolism of the rice phenylamide-type phytoalexins by rice pathogenic fungi　　　　　　　　　　　　　　　
隠塚修平; Surono Dwi Saputra; 戸嶋浩明; 長谷川守文
日本農薬学会第 45 回大会, 09 Mar. 2020, 日本農薬学会
20200308, 20200310

イネのアミド型ファイトアレキシンの病原真菌による代謝　　　　　　　　　　　　　　　
隠塚修平; 木村塁; 香川明慶; 西脇萌; 戸嶋浩明; 長谷川 守文
日本農芸化学会 2019 年度大会, 24 Mar. 2019, 日本農芸化学会

Comparative genomic insights into the degradation of endocrine disrupting nonylphenols by the two Sphingobium strains　　　　　　　　　　　　　　　
Mina Ootsuka; Yong Guo; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta
17th International Symposium on Microbial Ecology, Aug. 2018, International Society for Microbial Ecology

Molecular characterization of opdA homologs and nmoA, genes involved in nonylphenol degradation　　　　　　　　　　　　　　　
Mina Ootsuka; Yoko Yoshioka-Ikunaga; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta
日本微生物生態学会第 32 回大会, 11 Jul. 2018, 日本微生物生態学会

イネフィトアレキシンであるモミラクトン A のイネ病原真菌による代謝　　　　　　　　　　　　　　　
隠塚修平; 勝間田駿; 武山恵典; 戸嶋浩明; 長谷川守文
日本農薬学会第 43 回大会, 26 May 2018, 日本農薬学会

茨城県における芍薬の有機・草生栽培でのリビングマルチ利用について　　　　　　　　　　　　　　　
関浩一; 小松崎将一; 長谷川守文
日本農作業学会 2018 年度春季大会, 17 Mar. 2018, 日本農作業学会

イネ紋枯病菌によるフィトアレキシン代謝産物の構造決定及び抗菌活性　　　　　　　　　　　　　　　
勝間田駿; 隠塚修平; 戸嶋浩明; 長谷川守文
日本農芸化学会 2018 年度大会, 16 Mar. 2018, 日本農芸化学会

イネにおけるモミラクトン輸送体遺伝子の探索 – 第 2 報 –　　　　　　　　　　　　　　　
湊志帆; 松浦嵩; 黒田昌治; 菅野裕理; 瀬尾光範; 長谷川守文; 三橋渉; 岡田憲典; 豊増知伸
植物化学調節学会第 52 回大会, 29 Oct. 2017, 植物化学調節学会

Comparative analysis of alkylphenol degradation genes in Sphingobium amiense and Sphingobium cloacae　　　　　　　　　　　　　　　
Mina Ootsuka; Yoko Yoshioka-Ikunaga; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta
環境微生物系学会合同大会 2017, 30 Aug. 2017

植物病原菌に対するイネフィトアレキシンの抗菌活性　　　　　　　　　　　　　　　
勝間田駿; 大橋佳奈; 戸嶋浩明; 長谷川守文
日本農芸化学会 2017 年度大会, 18 Mar. 2017, 日本農芸化学会

Complete genome analysis of a nonylphenol-degrading bacterium Sphingobium cloacae JCM10874^T　　　　　　　　　　　　　　　
Mina Ootsuka; Yoko Yoshioka-Ikunaga; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta
日本微生物生態学会第 31 回大会, 23 Oct. 2016, 日本微生物生態学会

Evolution of biosynthetic gene clusters of diterpene phytoalexins in rice　　　　　　　　　　　　　　　
Tomonobu Toyomasu; Koji Miyamoto; Matthew R. Shenton; Morifumi Hasegawa; Hiroshi Kawaide; Wataru Mitsuhashi; Hisakazu Yamane; Nori Kurata; Kazunori Okada
22nd International Conference on Plant Growth Substances, 22 Jun. 2016, The International Plant Growth Substances Association

シクロペンタン導入型KODA類縁体の合成研究　　　　　　　　　　　　　　　
渡辺美聡; 横山峰幸; 長谷川守文; 戸嶋浩明
日本農芸化学会 2016 年度大会, 30 Mar. 2016, 日本農芸化学会

イネフィトアレキシン モミラクトンAのイネいもち病菌による代謝産物の同定　　　　　　　　　　　　　　　
武山恵典; 平石由貴; 遠藤尚; 今井卓也; 戸嶋浩明; 長谷川守文
日本農芸化学会 2016 年度大会, 29 Mar. 2016, 日本農芸化学会

トチュウに含まれる癌幹細胞特異的阻害剤Eucommicin Aの同定　　　　　　　　　　　　　　　
藤原綾香; 西真由子; 吉田茂男; 長谷川守文; 安間智慧子; 梁明秀; 鈴木義人
日本農芸化学会 2016 年度大会, 29 Mar. 2016, 日本農芸化学会

イネのジテルペン系フィトアレキシン生合成遺伝子クラスターの進化過程　　　　　　　　　　　　　　　
宮本皓司; 藤田雅丈; Matthew R. Shenton; 坂井亜莉里; 菅原千都; 川出洋; 長谷川守文; 三橋渉; 山根久和; 倉田のり; 岡田憲典; 豊増知伸
日本農芸化学会 2016 年度大会, 28 Mar. 2016, 日本農芸化学会

Genetic engineering for the overproduction of polyhydroxyburyrate in cyanobacteria　　　　　　　　　　　　　　　
Sayaka Hondo; Masatoshi Takahashi; Takashi Osanai; Mami Matsuda; Tomohisa Hasunuma; Akio Tazuke; Yoichi Nakahira; Shigeru Chohnan; Morifumi Hasegawa; Munehiko Asayama
Pacifichem 2015, Dec. 2015

イネにおけるモミラクトン輸送体遺伝子の探索　　　　　　　　　　　　　　　
松浦嵩; 千葉光浩; 菅野裕理; 瀬尾光範; 岡田憲典; 黒田昌治; 長谷川守文; 三橋渉; 豊増知伸
植物化学調節学会第 50 回大会, 25 Oct. 2015, 植物化学調節学会

野生イネ Oryza rufipogon におけるモミラクトンとファイトカサンの生合成　　　　　　　　　　　　　　　
岡田憲典; 宮本皓司; 藤田雅丈; Matthew R. Shenton; 菅原千都; 坂井亜莉里; 嶋根真奈美; 堀江清孝; 長谷川守文; 川出洋; 三橋渉; 野尻秀昭; 山根久和; 倉田のり; 豊増知伸
植物化学調節学会第 50 回大会, 25 Oct. 2015, 植物化学調節学会

イネのジテルペン型ファイトアレキシン生合成遺伝子クラスター形成の 進化機構　　　　　　　　　　　　　　　
宮本皓司; 藤田雅丈; Matthew R. Shenton; 菅原千都; 坂井亜莉里; 嶋根真奈美; 堀江清孝; 長谷川守文; 川出洋; 三橋渉; 野尻秀昭; 山根久和; 倉田のり; 岡田憲典; 豊増知伸
植物化学調節学会第 50 回大会, 25 Oct. 2015, 植物化学調節学会

野生イネ Oryza rufipogon におけるジテルペン環化酵素遺伝子　　　　　　　　　　　　　　　
坂井亜莉里; 菅原千都; 宮本皓司; 藤田雅丈; Matthew R. Shenton; 嶋根真奈美; 長谷川 守文; 川出洋; 三橋渉; 山根久和; 倉田のり; 岡田憲典; 豊増知伸
植物化学調節学会第 50 回大会, 25 Oct. 2015, 植物化学調節学会

Functional analyses of dehydrogenases involved in momilactones biosynthesis in rice　　　　　　　　　　　　　　　
Koji Miyamoto; Morifumi Hasegawa; Tomonobu Toyomasu; Hisakazu Yamane; Kazunori Okada
TERPNET 2015, 04 Jun. 2015

紫外線照射イネ葉に蓄積するアミド化合物とイネいもち病菌に対する抗菌活性　　　　　　　　　　　　　　　
堀江清孝; 酒井健伍; 奥木美咲; 戸嶋浩明; 長谷川守文
日本農芸化学会 2015 年度大会, 27 Mar. 2015, 日本農芸化学会

茨城県における芍薬の有機・草生栽培での薬用成分について　　　　　　　　　　　　　　　
関浩一; 小松﨑将一; 東達哉; 長谷川守文
日本農作業学会 2015（平成 27）年度春季大会, 19 Mar. 2015, 日本農作業学会

藻類バイオプラスチック PHB 高生産株の創生と特徴付け　　　　　　　　　　　　　　　
本堂彩花; 高橋正俊; 小山内崇; 松田真実; 蓮沼誠久; 長谷川守文; 中平洋一; 朝山宗彦
第 37 回日本分子生物学会年会, 27 Nov. 2014, 日本分子生物学会

高温処理によってタイム（Thymus vulgaris）から揮散するテルペノイドの同定および in vitro 抗菌活性　　　　　　　　　　　　　　　
江口ゆみ; チンタ・ユフィタ・ドゥイ; 長谷川守文; 佐藤達雄
園芸学会平成 26 年度秋季大会, 28 Sep. 2014, 園芸学会

イネのフィトアレキシンであるサクラネチンのいもち病菌による代謝　　　　　　　　　　　　　　　
濱名一穂; 戸嶋浩明; 長谷川守文
日本農芸化学会2014年度大会, 29 Mar. 2014, 日本農芸化学会

紫外線照射イネ葉からの新規ファイトカサン類縁体の同定　　　　　　　　　　　　　　　
井上靖乃; 堀江清孝; 戸嶋浩明; 長谷川守文
日本農芸化学会2014年度大会, 29 Mar. 2014, 日本農芸化学会

イネにおけるモミラクトン輸送体遺伝子の探索　　　　　　　　　　　　　　　
千葉光浩; 菅野裕理; 岡田憲典; 長谷川守文; 三橋渉; 瀬尾光範; 豊増知伸
日本農芸化学会2014年度大会, 28 Mar. 2014, 日本農芸化学会

Two major dehydrogenases are involved in momilactone biosynthesis in rice　　　　　　　　　　　　　　　
Koji Miyamoto; Morifumi Hasegawa; Hideaki Nojiri; Hisakazu Yamane; Kazunori Okada
第55回日本植物生理学会年会, 20 Mar. 2014, 日本植物生理学会

藻類バイオプラスチック PHB 高増産株の創生　　　　　　　　　　　　　　　
本堂彩花; 高橋正俊; 沼野節子; 北崎千富美; 長谷川守文; 朝山宗彦
日本農芸化学会関東支部 2013 年度支部大会, 22 Nov. 2013, 日本農芸化学会関東支部

イモチ病菌におけるキメラ型ジテルペン合成酵素遺伝子 −第 2 報−　　　　　　　　　　　　　　　
佐藤智美; 後藤麻予; 南栄一; 長谷川守文; 岡田憲典; 山根久和; 三橋渉; 豊増知伸
植物化学調節学会第48回大会, 31 Oct. 2013, 植物化学調節学会

イネにおけるモミラクトン生合成に関与するデヒドロゲナーゼの機能解析　　　　　　　　　　　　　　　
宮本皓司; 長谷川守文; 野尻秀昭; 山根久和; 岡田憲典
植物化学調節学会第48回大会, 31 Oct. 2013, 植物化学調節学会

シアナイドはジテルペノイド系ファイトアレキシン誘導を介していもち病抵抗性に寄与する　　　　　　　　　　　　　　　
光原一朗; 瀬尾茂美; 長谷川守文; 井上靖乃; 岡田憲典; 山根久和; 大橋祐子
平成 25 年度日本植物病理学会大会, 27 Mar. 2013, 日本植物病理学会

抗β酸化型 CP-KODA (3-oxo-CP-KODA) の合成研究　　　　　　　　　　　　　　　
大山秀芳; 中山ともみ; 長谷川守文; 戸嶋浩明
日本農芸化学会 2013 年度大会, 26 Mar. 2013, 日本農芸化学会

イネのフィトアレキシン生合成遺伝子ノックダウン変異体の特徴付け　　　　　　　　　　　　　　　
豊増知伸; 菅原千都; 臼井雅美; 清水崇史; 岡田憲典; 長谷川守文; 南栄一; 川名義明; 三橋渉; 山根久和
日本農芸化学会 2013 年度大会, 25 Mar. 2013, 日本農芸化学会

イモチ病菌におけるキメラ型ジテルペン合成酵素遺伝子　　　　　　　　　　　　　　　
佐藤智美; 後藤麻予; 南栄一; 長谷川守文; 岡田憲典; 山根久和; 三橋渉; 豊増知伸
植物化学調節学会第47回大会, 27 Oct. 2012, 植物化学調節学会

紫外線照射イネ葉からの新奇ジテルペン化合物 ent-10-oxodepressin の単離　　　　　　　　　　　　　　　
井上靖乃; 阪井美紀; 姚群; 谷本洋輔; 戸嶋浩明; 長谷川守文
植物化学調節学会第 46 回大会, 02 Nov. 2011, 植物化学調節学会

イネフィトアレキシン モミラクトン B のいもち病菌による代謝産物の同定　　　　　　　　　　　　　　　
今井卓也; 戸嶋浩明; 長谷川守文
植物化学調節学会第 46 回大会, 02 Nov. 2011, 植物化学調節学会

イネのサクラネチン生合成酵素 OsNOMT の単離・同定　　　　　　　　　　　　　　　
清水崇史; 林鳳秋; 長谷川守文; 岡田憲典; 野尻秀昭; 山根久和
植物化学調節学会第 46 回大会, 02 Nov. 2011, 植物化学調節学会

イネフィトアレキシンの化学－新規化合物の探索とイネいもち病菌による代謝－　　　　　　　　　　　　　　　
長谷川守文
日本植物病理学会関東部会第7回若手の会, 16 Sep. 2011, 日本植物病理学会関東部会若手の会, [Invited]

HWC1およびHWC2の相互作用で生じるイネ雑種弱勢における病害抵抗性関連遺伝子の発現およびファイトアレキシンの蓄積　　　　　　　　　　　　　　　
沖山友哉; 一谷勝之; 長谷川守文; 渡部信義; 久保山勉
日本育種学会第119回講演会, 30 Mar. 2011, 日本育種学会

HRにおいてエチレンと共に生成するシアナイドはイネのいもち病抵抗性に寄与する　　　　　　　　　　　　　　　
瀬尾茂美; 光原一朗; 焦鋒; 岩井孝尚; 長谷川守文; 大橋祐子
平成23年度日本植物病理学会大会, 28 Mar. 2011, 日本植物病理学会

紫外線照射イネ葉からの新奇ジテルペン化合物の単離　　　　　　　　　　　　　　　
井上靖乃; 阪井美紀; 姚群; 谷本洋輔; 戸嶋浩明; 長谷川守文
日本農芸化学会2011年度大会, 27 Mar. 2011, 日本農芸化学会

イネoscomt1変異株を用いたサクラネチン生合成酵素の精製　　　　　　　　　　　　　　　
清水崇史; 林鳳秋; 長谷川守文; 岡田憲典; 野尻秀昭; 山根久和
日本農芸化学会2011年度大会, 27 Mar. 2011, 日本農芸化学会

ルイス酸を用いた環化反応によるインドールテルペン類の合成研究　　　　　　　　　　　　　　　
井坂哲也; 長谷川守文; 戸嶋浩明
日本農芸化学会2011年度大会, 26 Mar. 2011, 日本農芸化学会

シクロプロパン環導入型KODA類縁体の立体異性体選択的合成　　　　　　　　　　　　　　　
下村伸; 長谷川守文; 横山峰幸; 伊福欧二; 山村庄亮; 戸嶋浩明
日本農芸化学会2011年度大会, 26 Mar. 2011, 日本農芸化学会

いもち病菌のジテルペン合成酵素に関する研究　　　　　　　　　　　　　　　
後藤麻予; 菅井佳宣; 長谷川守文; 岡田憲典; 山根久和; 塩野義人; 三橋渉; 川出洋; 豊増知伸
日本農芸化学会2011年度大会, 26 Mar. 2011, 日本農芸化学会

イネフィトアレキシン モミラクトン A のいもち病菌による代謝産物の同定　　　　　　　　　　　　　　　
今井卓也; 大橋祐子; 光原一朗; 瀬尾茂美; 戸嶋浩明; 長谷川守文
植物化学調節学会第 45 回大会, 02 Nov. 2010, 植物化学調節学会

ルイス酸を用いた環化反応による非天然型インドールアルカロイドの合成　　　　　　　　　　　　　　　
井坂哲也; 長谷川守文; 戸嶋浩明
日本化学会第4回関東支部大会, 30 Aug. 2010, 日本化学会関東支部

シクロプロパン環導入型 KODA 類縁体の立体異性体選択的合成法に関する研究　　　　　　　　　　　　　　　
下村伸; 長谷川守文; 横山峰幸; 伊福欧二; 山村庄亮; 戸嶋浩明
日本化学会第4回関東支部大会, 30 Aug. 2010, 日本化学会関東支部

Induced resistance against crown rot disease in strawberry by hot water treatment　　　　　　　　　　　　　　　
Widiastuti; A.; Sasaki; K.; Yoshino; M.; Saito; H.; Hasegawa; M.; Narisawa; K.; Nitta; Y.; Sato; T.
平成22年度日本植物病理学会大会, 19 Apr. 2010, 日本植物病理学会

イネフィトアレキシン モミラクトンAのイネいもち病菌による代謝　　　　　　　　　　　　　　　
今井卓也; 大橋祐子; 光原一朗; 瀬尾茂美; 戸嶋浩明; 長谷川守文
日本農芸化学会2010年度大会, 30 Mar. 2010, 日本農芸化学会

シクロプロパン環導入型KODA類縁体の立体異性体選択的合成法に関する研究　　　　　　　　　　　　　　　
下村伸; 長谷川守文; 横山峰幸; 伊福欧二; 山村 庄亮; 戸嶋浩明
日本農芸化学会2010年度大会, 29 Mar. 2010, 日本農芸化学会

イチゴ葉の温湯処理による炭疽病菌抵抗性の誘導　　　　　　　　　　　　　　　
佐々木克宗; Ani Widiastuti; 長谷川守文; 戸嶋浩明; 佐藤達雄
植物化学調節学会第44回大会, 30 Oct. 2009, 植物化学調節学会

作物に病害抵抗性を誘導する温湯処理装置の開発　　　　　　　　　　　　　　　
芳野未央子; 齊藤晴文; アニ・ウィディアストゥティ; 高田圭太; 長谷川守文; 佐藤達雄
園芸学会平成21年度秋季大会, 27 Sep. 2009, 園芸学会

アミノ酸修飾型キラルアミンによるγ-およびδ-ラクトンの光学分割　　　　　　　　　　　　　　　
湯本賢一; 長谷川守文; 戸嶋浩明
日本農芸化学会2009年度大会, 29 Mar. 2009, 日本農芸化学会

紫外線照射によって誘導されるイネ葉片中のジテルペン化合物の同定および定量　　　　　　　　　　　　　　　
長谷川守文; 姚群; 古池秀平; 戸嶋浩明
日本農芸化学会2009年度大会, 28 Mar. 2009, 日本農芸化学会

いもち病菌に感染した抵抗性および罹病性イネでのファイトアレキシンの蓄積とその分解　　　　　　　　　　　　　　　
長谷川守文; 光原一朗; 瀬尾茂美; 古賀仁一郎; 岡田憲典; 山根久和; 大橋祐子
平成21年度日本植物病理学会大会, 27 Mar. 2009, 日本植物病理学会

ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究　　　　　　　　　　　　　　　
増口潔; 浦上恵理子; 長谷川守文; 三宮一宰; 松本一朗; 山口五十麿; 浅見忠男; 鈴木義人
日本農薬学会第34回大会, 18 Mar. 2009, 日本農薬学会

紫外線照射イネ葉片からのストレス誘導性化合物の精製　　　　　　　　　　　　　　　
姚群; 阪井美紀; 戸嶋浩明; 長谷川守文
日本農芸化学会2008年度大会, 28 Mar. 2008, 日本農芸化学会

イネのphytoalexinであるsakuranetinの生合成に関与するnaringenin 7-O-methyltransferase遺伝子の探索　　　　　　　　　　　　　　　
林鳳秋; 岡田憲典; 岡田敦; 長谷川守文; 安西弘行; 児玉治; 野尻秀昭; 山根久和
日本農芸化学会2008年度大会, 27 Mar. 2008, 日本農芸化学会

アレンオキシド環化酵素およびオキソフィトジエン酸還元酵素の発現抑制イネ系統を用いたいもち病菌抵抗性の解析　　　　　　　　　　　　　　　
屋良朝紀; 八丈野孝; 長谷川守文; 楠見健介; 瀬尾茂美; 射場厚
第49回日本植物生理学会年会, 21 Mar. 2008, 日本植物生理学会

ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究　　　　　　　　　　　　　　　
増口潔; 浦上恵理子; 長谷川守文; 三宮一宰; 松本一朗; 山口五十麿; 浅見忠男; 鈴木義人
植物化学調節学会第42回大会, 29 Oct. 2007

Momilactone A and B uptake by Arabidopsis thaliana and their growth inhibitory effects　　　　　　　　　　　　　　　
Hiroya Kujime; Katsumi Ota; Morifumi Hasegawa; Hisahi Kato-Noguchi
4th Asia-Pacific Conference on Chemical Ecology, 12 Sep. 2007

シロイヌナズナによる成長抑制物質モミラクトンの取り込み　　　　　　　　　　　　　　　
久次米弘也; 太田勝巳; 長谷川守文; 加藤尚
日本雑草学会第46回大会, 15 Apr. 2007

リノール酸高含有形質転換イネにおけるいもち病菌抵抗性の解析　　　　　　　　　　　　　　　
屋良朝紀; 八丈野孝; Jean-Luc Montillet; 長谷川守文; 楠見健介; 瀬尾茂美; 射場厚
第48回日本植物生理学会年会, 28 Mar. 2007

穀類種子糊粉層におけるアラビノガラクタン蛋白質の機能解析　　　　　　　　　　　　　　　
増口潔; 浦上恵理子; 長谷川守文; 森昌樹; 浅見忠男; 鈴木義人
日本農芸化学会2007年度大会, 25 Mar. 2007

イネにおけるフィトアレキシンの根からの分泌　　　　　　　　　　　　　　　
加賀原拓真; 岡田憲典; 長谷川守文; 山根久和; 三橋渉; 佐々武史; 豊増知伸
日本農芸化学会2007年度大会, 25 Mar. 2007

イネにおけるファイトアレキシン生合成遺伝子クラスターの解析　　　　　　　　　　　　　　　
志村和広; 岡田敦; 軸丸裕介; 大友一子; 豊増知伸; 三橋渉; 佐々武史; 長谷川守文; 児玉治; 渋谷直人; 岡田憲典; 野尻秀昭; 山根久和
植物化学調節学会第41回大会, 31 Oct. 2006

モミラクトンＡとBの単離とその生長抑制活性　　　　　　　　　　　　　　　
加藤尚; 太田勝巳; 長谷川守文
第222回日本作物学会講演会, 29 Oct. 2006

イネのモミラクトン生合成酵素遺伝子クラスターの機能解析　　　　　　　　　　　　　　　
志村和広; 岡田敦; 軸丸裕介; 高光旭; 大友一子; 豊増知伸; 三橋渉; 佐々武史; 長谷川守文; 児玉治; 渋谷直人; 岡田憲典; 野尻秀昭; 山根久和
日本農芸化学会2006年度大会, 27 Mar. 2006

イネのフラボノイド系ファイトアレキシンのイネ及びいもち病菌による代謝　　　　　　　　　　　　　　　
長谷川守文; 北村博司; 児玉治
日本農芸化学会2006年度大会, 27 Mar. 2006

イネのOsATファミリー遺伝子と耐病性との関係　　　　　　　　　　　　　　　
森昌樹; 林長生; 長谷川守文
日本農芸化学会2006年度大会, 26 Mar. 2006

ジャスモン酸欠損イネを用いたイモチ病菌抵抗性の解析　　　　　　　　　　　　　　　
屋良朝紀; 服部泰之; 八丈野孝; 長谷川守文; 瀬尾茂美; 楠見健介; 射場厚
第47回日本植物生理学会年会, Mar. 2006

オキソフィトジエン酸合成能を欠損させた形質転換イネの作成　　　　　　　　　　　　　　　
服部泰之; 屋良朝紀; 八丈野孝; 長谷川守文; 楠見健介; 射場厚
第47回日本植物生理学会年会, Mar. 2006

Involvement of JA and reactive oxygen species in production of pytoalexins in suspension-cultured rice cells　　　　　　　　　　　　　　　
Yusuke Jikumaru; Atsushi Okada; Takeshi Yamaguchi; Eiichi Minami; Naoto Shibuya; Morifumi Hasegawa; Osanmu Kodama; Jinichiro Koga; Kenji Umemura; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane
Pacifichem 2005, 16 Dec. 2005

イネアクチベーションタギングにより単離された擬似病斑変異体の原因遺伝子OsAT1およびそのファミリー遺伝子の発現解析　　　　　　　　　　　　　　　
森昌樹; 林長生; 中村英光; 市川裕章; 長谷川守文
第28回日本分子生物学会年会, 07 Dec. 2005

イネコーヒー酸3-O-メチルトランスフェラーゼのクローニングおよび機能解析　　　　　　　　　　　　　　　
林鳳秋; 山野剛; 長谷川守文; 安西弘行; 川崎信二; 児玉治
植物化学調節学会第40回大会, Nov. 2005

イネのファイトアレキシン生合成酵素遺伝子クラスターの機能解析　　　　　　　　　　　　　　　
志村和広; 岡田敦; 軸丸裕介; 大友一子; 豊増知伸; 三橋 渉; 佐々武史; 長谷川守文; 児玉治; 渋谷直人; 岡田憲典; 野尻秀昭; 山根久和
植物化学調節学会第40回大会, Nov. 2005

セジロウンカの加害によってイネ体内に誘導される耐病性機構の解析　　　　　　　　　　　　　　　
佐藤雅; 菅野紘男; 長谷川守文; 光原一朗; 児玉治; 大橋祐子
平成17年度日本植物病理学会大会, 31 Mar. 2005

イネ幼苗におけるジベレリン（GA），ブラシノステロイド（BA）応答性チオニン遺伝子の解析　　　　　　　　　　　　　　　
北永行拡; 中村英光; 市川裕章; 長谷川守文; 浅見忠男; 吉田茂男; 鈴木義人; 山口五十麿
日本農芸化学会2005年度大会, 30 Mar. 2005

コロナチン結合タンパク質解明を目指した分子プローブの開発　　　　　　　　　　　　　　　
鈴木宗典; 渡邉吉則; 長谷川守文; 潘衛鋒; 児玉治; 戸嶋浩明
日本農芸化学会2005年度大会, 30 Mar. 2005

イネ葉中のストレス誘導性化合物 4’,5-dihydroxy-7-methoxyflavone (ゲンクワニン）　　　　　　　　　　　　　　　
北村博司; 長谷川守文; 児玉治
日本農芸化学会2005年度大会, 30 Mar. 2005

イネアクチベーションタギングにより単離された疑似病斑変異体の原因遺伝子OsAT1およびそのファミリー遺伝子の解析　　　　　　　　　　　　　　　
森昌樹; 林長生; 長谷川守文; 杉本和彦; 廣近洋彦; 菊池尚志
日本農芸化学会2005年度大会, 29 Mar. 2005

イネ培養細胞のフィトアレキシン生産におけるジャスモン酸と活性酸素種の関与　　　　　　　　　　　　　　　
軸丸裕介; 山口武志; 南栄一; 渋谷直人; 長谷川守文; 児玉治; 古賀仁一郎; 梅村賢司; 岡田憲典; 野尻秀昭; 山根久和
第46回日本植物生理学会年会, Mar. 2005

RNAi法により作製したジャスモン酸欠損イネの解析　　　　　　　　　　　　　　　
屋良朝紀; 服部泰之; 八丈野孝; 長谷川守文; 瀬尾茂美; 楠見健介; 射場厚
第46回日本植物生理学会年会, Mar. 2005

ジャスモン酸生合成遺伝子の発現を抑制した形質転換イネの作成　　　　　　　　　　　　　　　
服部泰之; 屋良朝紀; 八丈野孝; 長谷川守文; 楠見健介; 射場厚
第46回日本植物生理学会年会, Mar. 2005

Generation of activation tagging lines of rice and characterization of several mutants　　　　　　　　　　　　　　　
Masaki Mori; Chikako Tomita; Morifumi Hasegawa; Nagao Hayashi; Kazuhiko Sugimoto; Hitoshi Sekimoto; Hirohiko Hirochika; Shoshi Kikuchi
2nd Symposium on Rice Functional Genomics, 15 Nov. 2004

イネアクチベーションタギングにより単離された擬似病斑変異体及び短粒半わい性変異体の解析　　　　　　　　　　　　　　　
森昌樹; 冨田千賀子; 長谷川守文; 林長生; 杉本和彦; 関本均; 廣近洋彦; 菊池尚志
植物化学調節学会第39回大会, 28 Oct. 2004

コロナチン-,ジャスモノイド-,及びオクタデカノイド結合タンパク質解明プローブ,ジヒドロコロナチン　　　　　　　　　　　　　　　
鈴木宗典; 長谷川守文; 児玉治; 戸嶋浩明
植物化学調節学会第39回大会, 28 Oct. 2004

イネアクチベーションタギングにより得られた擬似病斑変異体及び短粒変異体の原因遺伝子解析　　　　　　　　　　　　　　　
森昌樹; 冨田千賀子; 長谷川守文; 林長生; 杉本和彦; 関本均; 廣近洋彦; 菊池尚志
日本育種学会第106回講演会, 21 Sep. 2004

イネのアクチベーションタギングにより得られた疑似病斑葉変異体における病害抵抗性　　　　　　　　　　　　　　　
森昌樹; 冨田千賀子; 林長生; 長谷川守文; 杉本和彦; 関本均; 廣近洋彦; 菊池尚志
日本植物生理学会2004年度年会, 29 Mar. 2004

セジロウンカの加害によってイネ体内に誘導される耐病性機構の生化学的解明：イネ体内に生成されるサリチル酸・ジャスモン酸・ファイトアレキシンの経時的変化　　　　　　　　　　　　　　　
菅野紘男; 佐藤雅; 長谷川守文; 児玉治
平成16年度日本植物病理学会大会, 29 Mar. 2004

ジャスモン酸結合タンパク質の単離・解析に向けた分子プローブ候補化合物の合成と生理活性検定　　　　　　　　　　　　　　　
軸丸裕介; 浅見忠男; 瀬戸秀春; 吉田茂男; 横山正; 小原直美; 長谷川守文; 児玉治 他
日本植物生理学会2004年度年会, 27 Mar. 2004

セジロウンカの加害によってイネ体内に誘導される耐病性機構の生化学的解明：イネ体内に生成されるサリチル酸・ジャスモン酸・ファイトアレキシンの経時的変化　　　　　　　　　　　　　　　
菅野紘男; 佐藤雅; 長谷川守文; 児玉治
第48回日本応用動物昆虫学会大会, 26 Mar. 2004

イネのアクチベーションタギングにより得られた疑似病斑葉変異体の解析　　　　　　　　　　　　　　　
森昌樹; 富田千賀子; 長谷川守文; 杉本和彦; 関本均; 廣近洋彦; 菊池尚志
植物化学調節学会第38回大会, 30 Oct. 2003

イネ葉中のジャスモノイル-CoAチオエステラーゼ活性　　　　　　　　　　　　　　　
長谷川守文; 坂本雄紀; 戸嶋浩明; 児玉治
植物化学調節学会第38回大会, 29 Oct. 2003

ヤーコン葉に含まれる抗細菌活性物質　　　　　　　　　　　　　　　
林鳳秋; 長谷川守文; 児玉治
日本農芸化学会2003年度大会, 02 Apr. 2003

イネにおける各種エリシターのジテルペン型ファイトアレキシン誘導活性　　　　　　　　　　　　　　　
岡田敦; 軸丸祐介; 趙銀敏; 梅村賢司; 古賀仁一郎; 兼目裕充; 豊増知伸; 佐々武史; 渋谷直人; 長谷川守文; 児玉治; 西山真; 山根久和
日本農芸化学会2003年度大会, 02 Apr. 2003

Sphingomonas amiensisによるアルキルフェノール分解に関する研究　　　　　　　　　　　　　　　
宮川修一; 生長陽子; 長谷川守文; 太田寛行; 児玉治
日本農薬学会第28回大会, 24 Mar. 2003

高濃度及び低濃度ノニルフェノール分解性Sphingomonas属細菌における分解特性の解析　　　　　　　　　　　　　　　
生長陽子; 高原義治; 久留主泰朗; 長谷川守文; 白井誠; 太田寛行
日本微生物生態学会2002年度大会, Oct. 2002

Sphingomonas sp. YT株によるp-ノニルフェノール分解に関する研究　　　　　　　　　　　　　　　
宮川修一; 生長陽子; 長谷川守文; 太田寛行; 児玉治
日本農薬学会第27回大会, 01 Apr. 2002

Biosynthesis of rice diterpene phytoalexin, momilactone A　　　　　　　　　　　　　　　
Anotai Atawong; Zhang Zhi Qiang; Morifumi Hasegawa; Hiroaki Toshima; Osamu Kodama
日本農薬学会第27回大会, 01 Apr. 2002

エリシター処理およびいもち病感染により生成するイネ葉揮発性成分　　　　　　　　　　　　　　　
小原直美; 長谷川守文; 児玉治
日本農芸化学会2002年度大会, 26 Mar. 2002

イネファイトアレキシンの生合成：3β-hydroxy-9β-pimara-7,15-dien-19,6β-olideからモミラクトンAへの変換　　　　　　　　　　　　　　　
Anotai Atawong; 長谷川守文; 児玉治
第38回植物化学シンポジウム, 01 Dec. 2001

エリシター処理およびいもち病感染により生成するイネ葉揮発性成分の分析　　　　　　　　　　　　　　　
小原直美; 河合博; 長谷川守文; 児玉治
日本農芸化学会関東支部2001年度大会, 20 Oct. 2001

イネにおけるジャスモン酸応答性遺伝子RERJ1の機能解析　　　　　　　　　　　　　　　
桐渕協子; 武田正敬; 杉森美帆; 軸丸裕介; 山口武志; 秋本千春; 南栄一; 小野寺治子; 宇垣正志; 田中宥司; 渋谷直人; 小原直美; 長谷川守文; 児玉治; 野尻秀明; 大森俊雄; 西山真; 山根久和
植物化学調節学会第36回大会, 09 Oct. 2001

ジャスモン酸結合タンパク質の単離・解析に向けたプローブの調製と生理活性　　　　　　　　　　　　　　　
軸丸裕介; 傍嶋宏行; 管野学; 浅見忠男; 瀬戸秀春; 藤岡昭三; 吉田茂男; 小原直美; 長谷川守文; 児玉治; 横山正; 西山真; 山根久和
植物化学調節学会第36回大会, 09 Oct. 2001

ニトベギク（Tithonia diversifolia）に含まれる植物生長抑制物質　　　　　　　　　　　　　　　
高橋美奈子; 長谷川守文; 児玉治; 小林勝一郎
日本雑草学会第40回大会, 15 Apr. 2001

Participation of dehydrogenase in momilactone A biosynthesis　　　　　　　　　　　　　　　
Anotai Atawong; Morifumi Hasegawa; Osamu Kodama
日本農薬学会第26回大会, 31 Mar. 2001

UV-B照射により樹木に蓄積する二次代謝産物の解析　　　　　　　　　　　　　　　
平野篤史; 松田真菜; 長谷川守文; 岡野通明; 児玉治
日本農芸化学会2001年度大会, 25 Mar. 2001

エリシター処理により生成するイネ葉揮発性成分の分析　　　　　　　　　　　　　　　
小原直美; 河合博; 長谷川守文; 児玉治
日本農芸化学会2001年度大会, 25 Mar. 2001

コムギにおけるベンゾキサジノン誘導体（HDMBOA-Glc）の誘導発現に関わるメチルトランスフェラーゼの性状解析　　　　　　　　　　　　　　　
及川彰; 石原亨; 長谷川守文; 児玉治; 岩村俶
日本農芸化学会2001年度大会, 25 Mar. 2001

Biosynthesis of rice diterpene phytoalexin momilactone A via the nonmevalonate pathway　　　　　　　　　　　　　　　
Anotai Atawong; Morifumi Hasegawa; Osamu Kodama
日本農芸化学会2001年度大会, 24 Mar. 2001

Induction of phytoalexin production by oligosaccharides in rice leaves　　　　　　　　　　　　　　　
Yumiko Nakazato; Hiroshi Kawai; Morifumi Hasegawa; Osamu Kodama
NIAR-COE/BRAIN/CREST Joint International Symposium, 16 Nov. 2000

アズキ黄化芽生え中に含まれるジベレリン結合タンパク質の精製　　　　　　　　　　　　　　　
井田健太郎; 中嶋正敏; 徐正君; 長谷川守文; Seung-Hyun Park; 冨岡克史; 室伏旭; 山口五十麿
植物化学調節学会第35回大会, 03 Nov. 2000

Benzoxazinone誘導体（HDMBOA-Glc）の誘導発現とその機構　　　　　　　　　　　　　　　
及川彰; 石原亨; 長谷川守文; 児玉治; 岩村俶
植物化学調節学会第35回大会, 02 Nov. 2000

Degradation of branched nonylphenol by Sphingomonas sp. strain YT　　　　　　　　　　　　　　　
Ynte de Vries; Yoshiharu Takahara; Yoko Ikunaga; Morifumi Hasegawa; Hiroyuki Ohta
日本微生物生態学会2000年度大会, Nov. 2000

アズキ黄化芽生え中に含まれるジベレリン結合タンパク質　　　　　　　　　　　　　　　
井田健太郎; 中嶋正敏; 徐正君; 中村英光; 蝶野真喜子; 三原健治; 長谷川守文; 室伏旭; 山口五十麿
日本農芸化学会2000年度大会, 01 Apr. 2000

Biosynthesis of rice diterpenoid phytoalexins　　　　　　　　　　　　　　　
Anotai Atawong; Morifumi Hasegawa; Osamu Kodama
日本農芸化学会2000年度大会, 01 Apr. 2000

イネ葉におけるオリゴ糖類によるファイトアレキシン合成の誘導　　　　　　　　　　　　　　　
中里由美子; 河合博; 長谷川守文; 児玉治
日本農薬学会第25回大会, 28 Mar. 2000

Gibberellin-binding protein in azuki bean seedling　　　　　　　　　　　　　　　
Masatohi Nakajima; Zheng-Jung Xu; Kentaro Ida; Kenji Mihara; Morifumi Hasegawa; Noboru Murofushi; Isomaro Yamaguchi
PGRSA, 2000

アズキ黄化芽生えに存在するジベレリン結合タンパク質の精製　　　　　　　　　　　　　　　
三原健治; 中山明; 長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
植物化学調節学会第33回大会, 15 Oct. 1999

Methionine-induced phytoalexin production in rice leaves　　　　　　　　　　　　　　　
Yumiko Nakazato; Morifumi Hasegawa; Shigeru Tamogami; Hiroshi Kawai; Osamu Kodama
XVI International Botanical Congress, Aug. 1999

Phytoalexin production by methionine in rice leaves　　　　　　　　　　　　　　　
Morifumi Hasegawa; Yumiko Nakazato; Shigeru Tamogami; Hiroshi Kawai; Osamu Kodama
XIVth International Plant Protection Congress, 27 Jul. 1999

アズキ黄化芽生えに存在するジベレリン結合タンパク質の精製　　　　　　　　　　　　　　　
中嶋正敏; 三原健治; 長谷川守文; 室伏旭; 山口五十麿
日本農芸化学会1999年度大会, 01 Apr. 1999

APCI-LC/MS-MSによるイネファイトアレキシンの定量法（APCI-LC/MS-MSによるモミラクトンA,Bおよびサクラネチンの分析）　　　　　　　　　　　　　　　
田母神繁; 長谷川守文; 中里由美子; 児玉治
日本農薬学会第24回大会, 26 Mar. 1999

アサガオ未熟種子における活性型ジベレリンの免疫組織化学　　　　　　　　　　　　　　　
中嶋正敏; 朴洗震; 長谷川守文; 山口五十麿; 室伏旭
植物化学調節学会第33回大会, 15 Oct. 1998

Immunohistochemistry of gibberellins in developing seeds of Pharbitis nil　　　　　　　　　　　　　　　
Masatoshi Nakajima; Seijin Park; Morifumi Hasegawa; Isomaro Yamaguchi; Noboru Murofushi
16th International Conference of Plant Growth Substances, 15 Aug. 1998

Purification of gibberellin-binding protein in etiolated azuki bean seedlings　　　　　　　　　　　　　　　
Kenji Mihara; Akira Nakayama; Morifumi Hasegawa; Masatoshi Nakajima; Isomaro Yamaguchi; Noboru Murofushi
16th International Conference of Plant Growth Substances, 15 Aug. 1998

Partial purification of gibberellin-binding protein from azuki bean seedlings　　　　　　　　　　　　　　　
Morifumi Hasegawa
Satellite Symposium of 16th IPGSA, 11 Aug. 1998, [Invited]

種子の成熟過程におけるジベレリンの局在に関する免疫化学的研究　　　　　　　　　　　　　　　
朴洗震; 長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
日本農芸化学会1998年度大会, 01 Apr. 1998

アズキ幼植物中のジベレリン結合タンパク質の精製　　　　　　　　　　　　　　　
三原健治; 和田英毅; 滝田桂司; 長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
日本農芸化学会1997年度大会, 01 Apr. 1997

抗GA₉抗体の調製と応用　　　　　　　　　　　　　　　
今村博臣; 中嶋正敏; 長谷川守文; 山口五十麿; 室伏旭
日本農芸化学会1997年度大会, 01 Apr. 1997

アサガオ種子におけるジベレリンの動態に関する免疫化学的研究　　　　　　　　　　　　　　　
朴洗震; 長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
日本農芸化学会1997年度大会, 01 Apr. 1997

アズキ幼植物中のジベレリン結合タンパク質の精製（４）　　　　　　　　　　　　　　　
和田英毅; 滝田桂司; 長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
日本農芸化学会1996年度大会, 31 Mar. 1996

イネ穎花におけるジベレリンの免疫組織化学　　　　　　　　　　　　　　　
長谷川守文; 橋本展誉; 張軍; 山口五十麿; 室伏旭
日本農芸化学会1995年度大会, 01 Aug. 1995

Immunohistochemical localization of gibberellins　　　　　　　　　　　　　　　
Morifumi Hasegawa; Nobuyoshi Hashimoto; Jun Zhang; Isomaro Yamaguchi; Noboru Murofushi
15th International Conference on Plant Growth Substances, Aug. 1995

イネ葯におけるジベレリンの免疫組織化学　　　　　　　　　　　　　　　
長谷川守文; 橋本展誉; 張軍; 山口五十麿; 室伏旭
植物化学調節学会第30回大会, Oct. 1994

ジベレリン配糖体を特異的に認識する抗体の調製　　　　　　　　　　　　　　　
三谷宣仁; 梁泳烈; 長谷川守文; 中嶋正敏; 横田孝雄; 山口五十麿; 室伏旭
日本農芸化学会1994年度大会, 02 Apr. 1994

イネ葯中に含まれる新規ジベレリン配糖体について　　　　　　　　　　　　　　　
長谷川守文; 中嶋正敏; 山口五十麿; 室伏旭
植物化学調節学会第28回大会, Oct. 1993

Semi-quantification of gibberellins in male sterile anthers of rice (Oryza sativa L.) by immunoassay　　　　　　　　　　　　　　　
Morifumi Hasegawa; Masatoshi Nakajima; Isomaro Yamaguchi; Kazuyoshi Takeda; Nobutaka Takahashi; Noboru Murofushi
Post-Symposium of IBC, Aug. 1993

Semi-quantification of gibberellins in male sterile anthers of rice (Oryza sativa L.) by immunoassay　　　　　　　　　　　　　　　
Morifumi Hasegawa; Masatoshi Nakajima; Isomaro Yamaguchi; Kazuyoshi Takeda; Nobutaka Takahashi; Noboru Murofushi
Post-Symposium of IBC, Aug. 1993

イネ温度感応性遺伝子雄性不稔種における内生ジベレリン　　　　　　　　　　　　　　　
須藤和久; 矢越常弘; 長谷川守文; 本多一郎; 柳沢忠; 丸山清明; 中嶋正敏; 山口五十麿; 室伏旭; 高橋信孝
日本農芸化学会1993年度大会, 01 Apr. 1993

イネ雄性不稔種における葯の内生ジベレリン　　　　　　　　　　　　　　　
長谷川守文; 中島正敏; 山口五十麿; 武田和義; 高橋信孝; 室伏旭
日本農芸化学会1993年度大会, 01 Apr. 1993

イネのフラボノイド系ファイトアレキシン生合成酵素の精製と性質　　　　　　　　　　　　　　　
長谷川守文; 児玉治; 赤塚尹巳
日本農芸化学会1992年度大会, 02 Apr. 1992

Induction of naringenin-7-O-methyltransferase in ultraviolet-irradiated rice leaves　　　　　　　　　　　　　　　
Morifumi Hasegawa; Osamu Kodama; Tadami Akatsuka
International Botanical Congress, Aug. 1991

イネのフラボノイド系ファイトアレキシン生合成酵素について　　　　　　　　　　　　　　　
長谷川守文; 児玉治; 赤塚尹巳
日本農芸化学会1991年度大会, 31 Mar. 1991

Dec. 2016, International Society of Chemical Ecology

Dec. 2008, American Society of Plant Biologists

American Chemical Society

The Weed Science Society of Japan

Pesticide Science Society of Japan

The Japanese Society for Chemical Regulation of Plants

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Identification of novel diterpenoid phytoalexins by using information of the biosynthetic genes in rice species.
Grant-in-Aid for Scientific Research (B)
Yamagata University
01 Apr. 2023 - 31 Mar. 2026

生合成遺伝子情報を利用したイネ類の新規ジテルペノイド型フィトアレキシンの同定　　　　　　　　　　　　　　　
基盤研究（B）
Apr. 2023 - Mar. 2026

植物の動的防御物質フィトアレキシンの構造的多様性と病原菌による代謝に関する研究　　　　　　　　　　　　　　　
基盤研究（C）
Apr. 2023 - Mar. 2026

Bio-organic chemical study on dynamic defense mechanisms of plants
Grant-in-Aid for Scientific Research (C)
Ibaraki University
2010 - 2012

イネのフラボノイド系ファイトアレキシン生合成酵素に関する研究
若手研究(B)
茨城大学
2004 - 2006

Biochemical and genetic analyses of nonylphenol degradation metabolism in environmental bacteria
Grant-in-Aid for Scientific Research (B)
IBARAKI UNIVERSITY
2001 - 2003

特異的光親和標識によるジベレリン結合タンパク質の精製
特別研究員奨励費
東京大学
1998 - 1998