Methanotrophic Communities and Cultivation of Methanotrophs from Rice Paddy Fields Fertilized with Pig-livestock Biogas Digestive Effluent and Synthetic Fertilizer in the Vietnamese Mekong Delta
Huynh Van Thao; Mitsunori Tarao; Hideshige Takada; Tomoyasu Nishizawa; Tran Sy Nam; Nguyen Van Cong; Do Thi Xuan, Japanese Society of Microbial Ecology
Microbes and Environments, 02 Oct. 2024, [Reviewed]

Methanotrophic Inoculation Reduces Methane Emissions from Rice Cultivation Supplied with Pig-Livestock Biogas Digestive Effluent
Huynh Van Thao; Mitsunori Tarao; Hideshige Takada; Tomoyasu Nishizawa; Tran Sy Nam; Nguyen Van Cong; Do Thi Xuan, Biogas digestive effluent (BDE) is a nutrient-enriched source that can be utilized as an organic fertilizer for rice cultivation without synthetic fertilizer (SF) application. However, a primary concern is the stimulation of methane (CH4) emissions due to the enrichment of the labile organic carbon, a favorite substrate of methanogenic archaea. Methanotrophs potentially reduce greenhouse gas (GHG) emissions from rice fields owing to metabolizing CH4 as a carbon source and energy. We therefore examined the effect of the application of methanotroph-inoculated BDE to the rice cultivated paddy soil on GHG emissions and rice productivity under a pot experiment. Methanotrophs (Methylosinus sp. and Methylocystis sp.), isolated from the Vietnamese Mekong Delta’s rice fields, were separately inoculated to the heated BDE, followed by a 5-day preincubation. Methanotroph-inoculated BDE was supplied to rice cultivation to substitute SF at 50% or 100% in terms of nitrogen amount. The results showed that the total CH4 emissions increased ~34% with the application of BDE. CH4 emissions were significantly reduced by ~17–21% and ~28–44% under the application of methanotroph-inoculated BDE at 100% and 50%, respectively. The reduction in CH4 was commensurate with the augmentation of pmoA transcript copy number under methanotroph-inoculated BDE. In addition, methanotroph-inoculated BDE application did not increase nitrous oxide (N2O) emissions and adversely affect rice growth and grain productivity. This study highlighted the BDE-recirculated feasibility for a lower CH4 emission rice production based on methanotrophs where high CH4-emitting fields were confirmed., MDPI AG
Agronomy, 27 May 2024, [Reviewed]

Tardiphaga alba sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil
Zhihua Bao; Chaojun Wang; Jiahui Cao; Tingting Zhang; Yong Guo; Yoshinori Sato; Tomoyasu Nishizawa; Hiroyuki Ohta, A previously undescribed, heavy-metal-tolerant, motile, Gram-negative bacterium, designated strain SK50-23^T, was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK50-23^T was closely related to Tardiphaga robiniae LMG 26467^T and the non-phototrophic ‘Rhodopseudomonas boonkerdii’ NS23^T (98.1 and 97.3 % 16S rRNA gene sequence similarity, respectively). Strain SK50-23^T possessed a circular genome of 5.86 Mb, with a DNA G+C content of 61.9 mol%. Digital DNA–DNA hybridization showed 20.8–21.6 % similarity between strain SK50-23^T and related species. In addition, the whole-genome average nucleotide identity values between strain SK50-23^T and related species ranged from 75.1 to 83.5 %. The major cellular fatty acid identified in strain SK50-23^T was C_18 : 1ω7c, and the main isoprenoid quinone present was ubiquinone Q-10. Strain SK50-23^T could be assigned to the genus Tardiphaga with the species name Tardiphaga alba sp. nov. based on morphological, chemotaxonomic and genome-based taxonomic characteristics, and 16S rRNA gene-based phylogenetic characteristics. The type strain of the proposed novel species is SK50-23^T (=NBRC 108825^T=CGMCC No. 1.12037^T)., Microbiology Society
International Journal of Systematic and Evolutionary Microbiology, 10 Jan. 2024, [Reviewed]

Dark septate endophytic fungi associated with pioneer grass inhabiting volcanic deposits and their functions in promoting plant growth
Han Sun; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Abstract. Growth of the pioneer grass Miscanthus condensatus, one of the first vegetation types to be established on volcanic deposits, is promoted by root-associated fungi, particularly dark septate endophytes (DSEs). Fungal taxa within DSEs colonize the root of Miscanthus condensatus in oligotrophic Andosol, and their function in plant growth promotion remains largely unknown. We, therefore, comprehensively assessed the composition of the DSE community associated with Miscanthus condensatus root in volcanic ecosystems using the approaches of both metabarcoding (next-generation sequencing) and isolation (culturing). Also, the promotion effects of DSEs on plant growth (rice as a proxy) were evaluated by inoculation of core isolates to rice roots. Here, we found the following: (i) 70 % of culturable fungi that colonized Miscanthus condensatus phylogenetically belonged to DSEs; (ii) seven orders were identified by both sequencing and culturing methods; and (iii) inoculation of DSE isolates (Phialocephala fortinii, P. helvetica, and Phialocephala sp.) validated their effects on rice growth, particularly under an extremely low pH condition (compared to the control without inoculation, rice biomass was enhanced 7.6-fold after inoculation of P. fortinii). This study helps improve our understanding of the community of Miscanthus condensatus-associated DSE fungi and their functions in promoting plant growth., Copernicus GmbH
Biogeosciences, 29 Nov. 2023, [Reviewed]

Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales
Takashima; Y.; K. Yamamoto; Y. Degawa; Y. Guo; T. Nishizawa; H. Ohta; K. Narisawa, Abstract

Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island in the Ogasawara (Bonin) Islands, Japan. The fungus was morphologically, phylogenetically, and physiologically characterized and proposed as a new species, Saksenaea boninensis sp. nov. Besides the fungal taxonomy, we also found the presence of BRE in isolates of this species by diagnostic PCR amplification of the 16S rRNA gene from mycelia, fluorescence microscopic observations, and isolation of the bacterium in pure culture. Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. The discovery of a culturable BRE lineage in the genus Saksenaea will add new insight into the evolutional origin of mucoralean fungus-BRE associations and emphasize the need to pay more attention to endofungal bacteria potentially associated with isolates of thermotolerant mucoralean fungi causing mucormycosis., BMC
IMA Fungus, Nov. 2023, [Reviewed]

Mitigation of methane emissions from paddy field by inoculating rice seeds with soil bacteria　　　　　　　　　　　　　　　
Sakoda; M.; T. Nishizawa, Corresponding, HOKURYUKAN
AGRICULTURAL BIOTECHNOLOGY, Nov. 2023, [Invited]

Complete genome sequence of Pseudoalteromonas sp. PS1M3, a psychrotrophic bacterium isolated from deep-sea sediment off the Boso Peninsula, Japan Trench
Yoshihito Nikaidou; Yong Guo; Mahoko Taguchi; Shigeru Chohnan; Tomoyasu Nishizawa; Yasurou Kurusu, Corresponding, Elsevier BV
Marine Genomics, Jun. 2023, [Reviewed]

Enhanced supply of acetyl-CoA by exogenous pantothenate kinase promotes synthesis of poly(3-hydroxybutyrate)
Hirotaka Kudo; Sho Ono; Kenta Abe; Mami Matsuda; Tomohisa Hasunuma; Tomoyasu Nishizawa; Munehiko Asayama; Hirofumi Nishihara; Shigeru Chohnan, Abstract

Background

Coenzyme A (CoA) is a carrier of acyl groups. This cofactor is synthesized from pantothenic acid in five steps. The phosphorylation of pantothenate is catalyzed by pantothenate kinase (CoaA), which is a key step in the CoA biosynthetic pathway. To determine whether the enhancement of the CoA biosynthetic pathway is effective for producing useful substances, the effect of elevated acetyl-CoA levels resulting from the introduction of the exogenous coaA gene on poly(3-hydroxybutyrate) [P(3HB)] synthesis was determined in Escherichia coli, which express the genes necessary for cyanobacterial polyhydroxyalkanoate synthesis (phaABEC).

Results

E. coli containing the coaA gene in addition to the pha genes accumulated more P(3HB) compared with the transformant containing the pha genes alone. P(3HB) production was enhanced by precursor addition, with P(3HB) content increasing from 18.4% (w/w) to 29.0% in the presence of 0.5 mM pantothenate and 16.3%–28.2% by adding 0.5 mM β-alanine. Strains expressing the exogenous coaA in the presence of precursors contained acetyl-CoA in excess of 1 nmol/mg of dry cell wt, which promoted the reaction toward P(3HB) formation. The amount of acetate exported into the medium was three times lower in the cells carrying exogenous coaA and pha genes than in the cells carrying pha genes alone. This was attributed to significantly enlarging the intracellular pool size of CoA, which is the recipient of acetic acid and is advantageous for microbial production of value-added materials.

Conclusions

Enhancing the CoA biosynthetic pathway with exogenous CoaA was effective at increasing P(3HB) production. Supplementing the medium with pantothenate facilitated the accumulation of P(3HB). β-Alanine was able to replace the efficacy of adding pantothenate., Springer Science and Business Media LLC
Microbial Cell Factories, 20 Apr. 2023, [Reviewed]

A Nitrate-Transforming Bacterial Community Dominates in the Miscanthus Rhizosphere on Nitrogen-Deficient Volcanic Deposits of Miyake-jima
Ahmad Arsyadi; Yong Guo; Akiko Ebihara; Nobuo Sakagami; Midori Sakoda; Kanako Tago; Takashi Kamijo; Hiroyuki Ohta; Tomoyasu Nishizawa, Last, The perennial gramineous grass Miscanthus condensatus functions as a major pioneer plant in colonizing acidic volcanic deposits on Miyake-jima, Japan, despite a lack of nitrogen nutrients. The nitrogen cycle in the rhizosphere is important for the vigorous growth of M. condensatus in this unfavorable environment. In the present study, we identified the nitrogen-cycling bacterial community in the M. condensatus rhizosphere on these volcanic deposits using a combination of metagenomics and culture-based analyses. Our results showed a large number of functional genes related to denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in the rhizosphere, indicating that nitrate-transforming bacteria dominated the rhizosphere biome. Furthermore, nitrite reductase genes (i.e., nirK and nirS) related to the denitrification and those genes related to DNRA (i.e., nirB and nrfA) were mainly annotated to the classes Alpha-proteobacteria, Beta-proteobacteria, and Gamma-proteobacteria. A total of 304 nitrate-succinate-stimulated isolates were obtained from the M. condensatus rhizosphere and were classified into 34 operational taxonomic units according to amplified 16S rRNA gene restriction fragment pattern analysis. Additionally, two strains belonging to the genus Cupriavidus in the class Beta-proteobacteria showed a high in vitro denitrifying activity; however, metagenomic results indicated that the DNRA-related rhizobacteria appeared to take a major role in the nitrogen cycle of the M. condensatus rhizosphere in recent Miyake-jima volcanic deposits. This study elucidates the association between the Miscanthus rhizosphere and the nitrate-reducing bacterial community on newly placed volcanic deposits, which furthers our understanding of the transformation of nitrogen nutrition involved in the early development of vegetation., MDPI AG
Microorganisms, 19 Jan. 2023, [Reviewed]

Mitigation of paddy field soil methane emissions by betaproteobacterium Azoarcus inoculation of rice seeds
Sakoda; M.; T. Tokida; Y. Sakai; K. Senoo; T. Nishizawa, Last, Sakoda, M., T. Tokida, Y. Sakai, K. Senoo, T. Nishizawa, JSME, JSSM, TSME, JSPMI, JSE
Microbes Environ., Dec. 2022, [Reviewed]

Long-term no-tillage and rye cover crop affect soil biological indicators on Andosols in a humid, subtropical climate
Gong; Y.; P. Li; Y. Guo; H. Aso; Q. Huang; H. Araki; T. Nishizawa; M. Komatsuzaki, WILEY
Eur. J. Soil Sci., Oct. 2022, [Reviewed]

Complete genome sequence of a psychrophilic bacterium, Pseudoalteromonas sp. strain APM04, isolated from the seafloor of the South Mariana Trough, Pacific Ocean
Taguchi; M.; Y. Guo; T. Nishizawa; S. Chohnan; Y. Kurusu, Corresponding, The complete genome sequence of Pseudoalteromonas sp. strain APM04, which is a psychrophilic bacterium that inhabits the seabed of the South Mariana Trough, Pacific Ocean, was determined to characterize the genetic features associated with evolution in extremophilic and oligotrophic deep seawater., Am Soc Microbiol
Microbiol. Resour. Ann., Aug. 2022, [Reviewed]

No Tillage Increases SOM in Labile Fraction but Not Stable Fraction of Andosols from a Long-Term Experiment in Japan
Jeannette Aduhene-Chinbuah; Soh Sugihara; Masakazu Komatsuzaki; Tomoyasu Nishizawa; Haruo Tanaka, No tillage (NT) fosters carbon (C) sequestration, increases soil organic matter (SOM) stock, and improves soil health. However, its effect on SOM accumulation in Andosol, which has high OM stabilization characteristics due to its specific mineral properties, remains unclear. In this study, we evaluated the effect of NT on SOM content and its distribution by the physical fractionation method and assessed the quality of accumulated SOM in each fraction. We collected soil samples at 0–2.5, 2.5–7.5, and 7.5–15 cm depths from NT and conventional tillage (CT) plots in a long-term (19 years) field experiment of Andosols in Ibaraki, Japan. The soil samples were separated into light fraction (LF), coarse-POM (cPOM: 0.25–2 mm), fine-POM (fPOM: 0.053–0.25 mm), and silt + clay (mOM: <0.053 mm). The C, nitrogen (N), and organic phosphorus (Po) contents of each fraction were analyzed. The C content of cPOM and fPOM in NT at 0–7.5 cm was higher than in CT, while there was no clear difference in the mOM fraction or deeper layer (7.5–15 cm). NT increased the C, N, and Po contents in the labile POM fractions at the surface layers but did not increase the stable fraction or change the quality., MDPI AG
Agronomy, 14 Feb. 2022, [Reviewed]

Whole-Genome Sequence of Entomortierella parvispora E1425, a Mucoromycotan Fungus Associated with Burkholderiaceae -Related Endosymbiotic Bacteria
Afri Herlambang; Yong Guo; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta; Tomoyasu Nishizawa, Last, Some mucoromycotan fungi establish symbiotic associations with endohyphal bacteria. Here, the genome of Entomortierella parvispora E1425 (synonymously known as Mortierella parvispora E1425), which harbors a cultured Burkholderiaceae -related endobacterium (BRE) designated Mycoavidus sp. Strain B2-EB, was sequenced. We provide genomic information to elucidate fungal-BRE symbiotic features., American Society for Microbiology
Microbiology Resource Announcements, 20 Jan. 2022, [Reviewed]

Complete Genome Sequence of a Chemolithoautotrophic Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans Strain NFP31, Isolated from Volcanic Ash Deposits on Miyake-Jima, Japan
Tatsuhiro Kato; Yong Guo; Reiko Fujimura; Takamichi Nakamura; Tomoyasu Nishizawa; Yasuro Kurusu; Hiroyuki Ohta, Corresponding, The genome sequence of Acidithiobacillus ferrooxidans strain NFP31, which is a chemolithoautotrophic iron-oxidizing bacterium that inhabits acidified volcanic deposits on Mount Oyama, Miyake Island (Miyake-jima), Japan, was determined to identify the genetic characteristics associated with pioneer microbes in newly placed pyroclastic deposits., American Society for Microbiology
Microbiology Resource Announcements, 20 Jan. 2022, [Reviewed]

Root bacteriome of a pioneer grass Miscanthus condensatus along restored vegetation on recent Miyake-jima volcanic deposits
Yong Guo; Tomoyasu Nishizawa; Nobuo Sakagami; Reiko Fujimura; Takashi Kamijo; Hiroyuki Ohta, Corresponding, Elsevier BV
Rhizosphere, Sep. 2021, [Reviewed]

Soil microbes and climate change
Hiroyuki Ohta; Tomoyasu Nishizawa, 日本土壌微生物学会
土と微生物, Apr. 2021, [Reviewed], [Invited]

糸状菌に内生する細菌の縮小化ゲノムの特徴
郭永・高島勇介・西澤智康, Last, JAPAN BIOSCIENCE ASSOCIATION
BIOSCIENCE & INDUSTRY, Mar. 2021, [Invited]

Mycoavidus sp. Strain B2-EB: Comparative Genomics Reveals Minimal Genomic Features Required by a Cultivable Burkholderiaceae -Related Endofungal Bacterium
Yong Guo; Yusuke Takashima; Yoshinori Sato; Kazuhiko Narisawa; Hiroyuki Ohta; Tomoyasu Nishizawa, Last, This study attempted the isolation of a novel endobacterium, Mycoavidus sp. B2-EB (JCM 33615), harbored in the fungal host Mortierella parvispora E1425 (JCM 39028). We report the complete genome sequence of this strain, which possesses a reduced genome size with relatively high genome completeness and a streamlined genome structure. The information indicates the minimal genomic features required by both the endofungal lifestyle and artificial cultivation, which furthers our understanding of genome reduction in fungal endosymbionts and extends the culture resources for biotechnological development on engineering synthetic microbiomes., American Society for Microbiology
Applied and Environmental Microbiology, Sep. 2020, [Reviewed]

土壌団粒構造と土壌プロセス2—実測と理論の統合を目指して—
荒井見和; 多胡香奈子; 和穎朗太; 取出伸夫; 青山正和; 西澤智康; 金子信博; 松岡健介; 太田寛行, 一般社団法人日本土壌肥料学会
日本土壌肥料学雑誌, Aug. 2020, [Reviewed], [Invited]

Aposymbiosis of a <i>Burkholderiaceae</i>-Related Endobacterium Impacts on Sexual Reproduction of Its Fungal Host
Yusuke Takashima; Yousuke Degawa; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Japanese Society of Microbial Ecology
Microbes and Environments, 2020, [Reviewed]

Genome sequence of Novoherbaspirillum sp. UKPF54, the plant growth-promoting rhizobacterial strain with N₂O-mitigation abilities, isolated from a paddy soil
Gao; N.; C. Zhou; W. Shen; S. Ota; Y. Shiratori; T. Nishizawa; K. Isobe; X. He; H. Ying; K. Senoo, Am Soc Microbiol
Microbiol. Resour. Ann., Jan. 2020, [Reviewed]

Elucidation of Biological Interactions of Endohyphal Bacteria within Fungal Hyphae
Tomoyasu Nishizawa, Institute for Fermentation, Osaka
IFO Res. Commun., Dec. 2019, [Reviewed], [Invited]

Genome Sequence of Arthrobacter sp. UKPF54-2, a Plant Growth-Promoting Rhizobacterial Strain Isolated from Paddy Soil
Weishou Shen; Xinchun Yu; Nan Gao; Sayuri Ota; Yutaka Shiratori; Tomoyasu Nishizawa; Kazuo Isobe; Xinhua He; Keishi Senoo, Arthrobacter sp. strain UKPF54-2, a plant growth-promoting rhizobacterium having the potential ability to control fungal and bacterial pathogens, was isolated from paddy soil in Kumamoto, Japan. We report here the whole-genome sequence of this strain., American Society for Microbiology
Microbiology Resource Announcements, 07 Nov. 2019, [Reviewed]

Azoarcus sp. strain KH32C affects rice plant growth and the root-associated soil bacterial community in low nitrogen input paddy fields
Midori Sakoda; Maika Mizusawa; Fumitaka Shiotsu; Nobuo Sakagami; Yong Guo; Yuji Masutomi; Daichi Fujii; Kazuo Isobe; Toru Fujiwara; Keishi Senoo; Tomoyasu Nishizawa, Last, Informa UK Limited
Soil Science and Plant Nutrition, 03 Sep. 2019, [Reviewed]

Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N ₂ O Mitigation Abilities
Nan Gao; Weishou Shen; Tomoyasu Nishizawa; Kazuo Isobe; Yong Guo; Hanjie Ying; Keishi Senoo, Azospirillum sp. strains TSA2S and TSH100 are plant growth-promoting rhizobacteria with the capacity to mitigate N ₂ O from agricultural soil. They were isolated from the rhizosphere of paddy soil in Tokyo, Japan. Here, we present the genome sequences of these two strains., American Society for Microbiology
Microbiology Resource Announcements, 08 Aug. 2019, [Reviewed]

Comparative Characterization of Bacterial Communities in Moss-Covered and Unvegetated Volcanic Deposits of Mount Merapi, Indonesia
Annisa N. Lathifah; Yong Guo; Nobuo Sakagami; Wataru Suda; Masanobu Higuchi; Tomoyasu Nishizawa; Irfan D. Prijambada; Hiroyuki Ohta, Japanese Society of Microbial Ecology
Microbes and Environments, 2019, [Reviewed]

Comparative Analysis of the Genetic Basis of Branched Nonylphenol Degradation by Sphingobium amiense DSM 16289T and Sphingobium cloacae JCM 10874T
Mina Ootsuka; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta, Japanese Society of Microbial Ecology
Microbes and Environments, Dec. 2018, [Reviewed]

Prevalence and intra-family phylogenetic divergence of Burkholderiaceae-related endobacteria associated with species of Mortierella
Takashima; Y.; K. Seto; Y. Degawa; Y. Guo; T. Nishizawa; H. Ohta; K. Narisawa, JSME, JSSM
Microbes Environ., Dec. 2018, [Reviewed]

植物－土壌脱窒細菌間相互作用を担う新奇シグナル物質の同定
西澤智康, Lead, Takano Life Science Research Foundation
Annual Research Report 2017, Dec. 2018, [Invited]

The distribution of antibiotic-resistant bacteria in eutrophic Lake Kasumigaura and neighboring agricultural fields
矢用もも・平野明則・松田祐輝・上原研人・今井達也・島袋 真・黒田久雄・太田寛行・西澤智康, Corresponding, 17th World Lake Conference
17th World Lake Conference, Lake Kasumigaura, Oct. 2018

Effect of denitrifying bacteria on rice plant growth-promotion under low-nitrogen input condition: Investigation of rice plant growth and molecular analysis of root-associated soil bacterial community
迫田 翠・水澤舞花・塩津文隆・郭 永・坂上伸生・滝本貴弘・増冨祐司・藤原 徹・妹尾啓史・西澤智康, Corresponding, 17th World Lake Conference
17th World Lake Conference, Lake Kasumigaura, Oct. 2018

Soil environmental condition and vegetation recovery of the abandoned cropland around Komado-shitsugen moor, Fukushima prefecture
Sakagami; N.; Y. Guo; Y. Takashima; T. Nishizawa; K. Narisawa; M. Watanabe, 17th World Lake Conference
17th World Lake Conference, Lake Kasumigaura, Oct. 2018

Elemental composition and microbial community structure analysis of surface soils affected by the Kanto-Tohoku heavy rainfall disaster in September 2015
齋藤明葉・坂上伸生・成澤才彦・伊藤哲司・西澤智康, Corresponding, 17th World Lake Conference
17th World Lake Conference, Lake Kasumigaura, Oct. 2018

Presence of Cu-type (NirK) and cd₁-type (NirS) nitrite reductase genes in a denitrifying bacterium Bradyrhizobium nitroreducens sp. nov.
Jeonghwan J.; N. Ashida; A. Kai; K. Isobe; T. Nishizawa; S. Otuka; A. Yokota; K. Senoo; S. Ishii, JSME, JSSM
Microbes Environ., Oct. 2018, [Reviewed]

Complete genome sequence of a microcystin-degrading bacterium Sphingosinicella microcystinivorans strain B-9
Jin; H.; T. Nishizawa; Y. Guo; A. Nishizawa; H.-D. Park; H. Kato; K. Tsuji; K.-I. Harada, Am Soc Microbiol
Microbiol. Resour. Ann., Sep. 2018, [Reviewed]

Complete genome sequence of Agrobacterium pusense VsBac-Y9, a bacterial symbiont of the dark septate endophytic fungus Veronaeopsis simplex Y34 with potential for improving fungal colonization in roots
Guo; Y.; Y. Matsuoka; T. Miura; T. Nishizawa; H. Ohta; K. Narisawa, Elsevier
J. Biotechnol., Aug. 2018, [Reviewed]

Complete Genome Sequence of the Nonheterocystous Cyanobacterium Pseudanabaena sp. ABRG5-3.
Naoyuki Tajima; Yu Kanesaki; Shusei Sato; Hirofumi Yoshikawa; Fumito Maruyama; Ken Kurokawa; Hiroyuki Ohta; Tomoyasu Nishizawa; Munehiko Asayama; Naoki Sato, We report here the complete sequences of the main genome (4.8 Mb) and seven plasmids of the semifilamentous, nonheterocystous cyanobacterium Pseudanabaena sp. ABRG5-3, a strain isolated from a pond in Japan. These data are expected to enhance our understanding of the Pseudanabaena subclade near the root of cyanobacterial diversity., Am Soc Microbiol
Genome announcements, 08 Feb. 2018, [Reviewed]

Effects of Rhizobium Species Living with the Dark Septate Endophytic Fungus Veronaeopsis simplex on Organic Substrate Utilization by the Host
Yong Guo; Yuuto Matsuoka; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Japanese Society of Microbial Ecology
Microbes and Environments, 2018, [Reviewed]

Comparative Genomic Insights into Endofungal Lifestyles of Two Bacterial Endosymbionts, Mycoavidus cysteinexigens and Burkholderia rhizoxinica
Dilruba Sharmin; Yong Guo; Tomoyasu Nishizawa; Shoko Ohshima; Yoshinori Sato; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta, Japanese Society of Microbial Ecology
Microbes and Environments, 2018, [Reviewed]

FVIIa-sTF and Thrombin Inhibitory Activities of Compounds Isolated from Microcystis aeruginosa K-139
Andrea Roxanne J. Anas; Akane Mori; Mineka Tone; Chiaki Naruse; Anna Nakajima; Hirohiko Asukabe; Yoshiaki Takaya; Susumu Y. Imanishi; Tomoyasu Nishizawa; Makoto Shirai; Ken-ichi Harada, The rise of bleeding and bleeding complications caused by oral anticoagulant use are serious problems nowadays. Strategies that block the initiation step in blood coagulation involving activated factor VII-tissue factor (fVIIa-TF) have been considered. This study explores toxic Microcystis aeruginosa K-139, from Lake Kasumigaura, Ibaraki, Japan, as a promising cyanobacterium for isolation of fVIIa-sTF inhibitors. M. aeruginosa K-139 underwent reversed-phase solid-phase extraction (ODS-SPE) from 20% MeOH to MeOH elution with 40%-MeOH increments, which afforded aeruginosin K-139 in the 60% MeOH fraction; micropeptin K-139 and microviridin B in the MeOH fraction. Aeruginosin K-139 displayed an fVIIa-sTF inhibitory activity of similar to 166 mu M, within a 95% confidence interval. Micropeptin K-139 inhibited fVIIa-sTF with EC50 10.62 mu M, which was more efficient than thrombin inhibition of EC50 26.94 mu M. The thrombin/fVIIa-sTF ratio of 2.54 in micropeptin K-139 is higher than those in 4-amidinophenylmethane sulfonyl fluoride (APMSF) and leupeptin, when used as positive controls. This study proves that M. aeruginosa K-139 is a new source of fVIIa-sTF inhibitors. It also opens a new avenue for micropeptin K-139 and related depsipeptides as fVIIa-sTF inhibitors., MDPI AG
MARINE DRUGS, Sep. 2017, [Reviewed]

Nitrous oxide (N2O)-reducing denitrifier-inoculated organic fertilizer mitigates N2O emissions from agricultural soils
Nan Gao; Weishou Shen; Estefania Camargo; Yutaka Shiratori; Tomoyasu Nishizawa; Kazuo Isobe; Xinhua He; Keishi Senoo, Springer Science and Business Media LLC
Biology and Fertility of Soils, 07 Aug. 2017, [Reviewed]

石人山古墳装飾石棺表面に形成した着生生物群集の構造解析
佐藤嘉則; 西澤智康; 小沼奈那美; 犬塚将英; 森井順之; 木 川りか; 朽津信明, Sekijinsan Tumulus in Hirokawa, Fukuoka prefecture is a keyhole-shaped burial mound with a decorated stone sarcophagus that was built in the first half of the 5th century. Recently, green epiphytic organisms have spread widely on the western side of the sarcophagus surface, preventing preservation and exhibition to the public. The aim of the present study was to analyze the epiphytic microbial community by using molecular techniques to determine the algal, fungal and bacterial community structures on the sarcophagus surface. The algal gene sequences closely related to those of nitrogen-fixing Tolypothrix distorta and salt-tolerant Halospirulina were detected. The environmental factors of limited nitrogen source,solar radiation,and dry and salty conditions were considered to have contributed to the ability of these algae to grow on the sarcophagus surface.Moreover, algal gene sequences closely related to that of Cyanidium were detected. The genus Cyanidium is a red algal extremophile which typically grows in acidic hot springs. It is known that organisms related to such an evolutionarily old red alga are able to grow on the stone sarcophagus surface. In fungal community structure analysis, sequences closely related to Mariannaea elegans and Trichoderma virens were commonly and frequently observed. In bacterial community structure analysis, Arthrobacter russicus, Sphingomonas leidyi and Brevundimonas vesicularis were commonly detected. In the present study,by using a molecular biology approach,the community structures of microorganisms on the stone sarcophagus surface were clarified. However, these community structures are expected to change even under natural conditions,depending on the surrounding environment that is influenced by annual and seasonal fluctuations or the growth of trees. With these changes, it is important to conduct ongoing surveys while continuing studies on biological control methods., 保存科学研究センター
保存科学, Mar. 2017, [Reviewed]

Actinophage R4 integrase-based site-specific chromosomal integration of non-replicative closed circular DNA
Takamasa Miura; Akito Nishizawa; Tomoyasu Nishizawa; Munehiko Asayama; Makoto Shirai, The actinophage R4 integrase (Sre)-based molecular genetic engineering system was developed for the chromosomal integration of multiple genes in Escherichia coli. A cloned DNA fragment containing two attP sites, green fluorescent protein (gfp) as a first transgene, and an antibiotic resistance gene as a selection marker was self-ligated to generate non-replicative closed circular DNA (nrccDNA) for integration. nrccDNA was introduced into attB-inserted E. coli cells harboring the plasmid expressing Sre by electroporation. The expressed Sre catalyzed site-specific integration between one of the two attP sites on nrccDNA and the attB site on the E. coli chromosome. The integration frequency was affected by the chromosomal location of the target site. A second nrccDNA containing two attB sites, lacZ encoding the alpha fragment of -galactosidase as a transgene, and another antibiotic resistance gene was integrated into the residual attP site on the gfp-integrated E. coli chromosome via one of the two attB sites according to reiterating site-specific recombination. The integrants clearly exhibited -galactosidase activity and green fluorescence, suggesting the simultaneous expression of multiple recombinant proteins in E. coli. The results of the present study showed that a step-by-step integration procedure using nrccDNA achieved the chromosomal integration of multiple genes., WILEY-BLACKWELL
JOURNAL OF BASIC MICROBIOLOGY, Jun. 2016, [Reviewed]

Inoculation with nitrous oxide (N2O)-reducing denitrifier strains simultaneously mitigates N2O emission from pasture soil and promotes growth of pasture plants
Nan Gao; Weishou Shen; Hiroko Kakuta; Nobuhiro Tanaka; Toru Fujiwara; Tomoyasu Nishizawa; Noriko Takaya; Tadashi Nagamine; Kazuo Isobe; Shigeto Otsuka; Keishi Senoo, The aim of this study was to screen nitrous oxide (N2O)-reducing denitrifier strains showing both N2O mitigation and plant growth-promoting (PGP) effects in soil systems, and the effects of selected strains were monitored in soil and plant, analyzed, and comparatively evaluated. Forty denitrifier strains affiliated with Azospirillum and Herbaspirillum, previously isolated from three different paddy soils, were evaluated. Of these, 11 produced indole-3-acetic acid (>5 jig mL(-1)), 9 promoted the growth of red clover (Trifolium pratense L. var. Medium) or timothy (Phleum pratense L. var. Horizon) on agar plates, and 7 were inoculated into two different soils for cultivating red clover and timothy in a greenhouse. Compared with non-inoculated control, N2O flux from red clover soil and from timothy soil were significantly lower 8 and 14 days and 9 days onwards, respectively, after inoculation with these seven strains. Cumulative N2O emissions from red clover soil were significantly lowered through inoculation with these seven strains. The growth parameters, including plant height, leaf area, fresh weight or dry weight, of the two pasture plants were significantly greater in soils inoculated with most of these seven strains than in non inoculated soils. The uptake of C and N by the two pasture plants was significantly greater in soils inoculated with most of these seven strains than in non-inoculated soils. In conclusion, inoculating N2O reducing denitrifiers to pasture soil could mitigate N2O emissions and simultaneously promote the growth of pasture plants in a greenhouse. These strains will be invaluable microbiological resources for developing novel biofertilizers. (C) 2016 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
SOIL BIOLOGY & BIOCHEMISTRY, Jun. 2016, [Reviewed]

Mycoavidus cysteinexigens gen. nov., sp nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata
Shoko Ohshima; Yoshinori Sato; Reiko Fujimura; Yusuke Takashima; Moriyuki Hamada; Tomoyasu Nishizawa; Kazuhiko Narisawa; Hiroyuki Ohta, An endohyphal bacterium (strain B1-EBT) living in association with the fungus Mortierella elongata FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EBT required cysteine for growth and grew at temperatures between 4 and 35 degrees C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EBT forms a distinct clade in the family Burkholderiaceae, encompassing a group of endosymbionts associated with several soil isolates of M. elongata. The most closely related genus is 'Candidatus Glomeribacter gigasporarum', an endosymbiont of the arbuscular mycorrhizal fungus Gigaspora margarita. The major cellular fatty acids of strain B1-EBT were C-16 : 0, summed feature 3 (C-16 : (1)omega 7c and C-16 : (1)omega 6c) and summed feature 8 (C-18 : (1)omega 7c or C-18 : (1)omega 6c). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EBT represents a novel genus and novel species in the family Burkholderiaceae, for which the name Mycoavidus cysteinexigens gen. nov., sp. nov. is proposed. The type strain is B1-EBT (=JCM 30646(T) =LMG 28693(T) =NBRC 110909(T))., SOC GENERAL MICROBIOLOGY
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, May 2016, [Reviewed]

Complete genome sequence of Streptomyces parvulus 2297, integrating sitespecifically with actinophage R4
Tomoyasu Nishizawa; Takamasa Miura; Chizuko Harada; Yong Guo; Kazuhiko Narisawa; Hiroyuki Ohta; Hideo Takahashi; Makoto Shirai, Streptomyces parvulus 2297, which is a host for site-specific recombination according to actinophage R4, is derived from the type strain ATCC 12434. Species of S. parvulus are known as producers of polypeptide antibiotic actinomycins and have been considered for industrial applications. We herein report for the first time the complete genome sequence of S. parvulus 2297., American Society for Microbiology
Genome Announcements, 2016, [Reviewed]

Complete genome sequence of the nonylphenol-degrading bacterium Sphingobium cloacae JCM 10874T
Mina Ootsuka; Tomoyasu Nishizawa; Hiroyuki Ohta, Sphingobium cloacae JCM 10874T can degrade phenolic endocrine-disrupting chemicals, nonylphenol, and octylphenol. Here, we report the complete genome sequence of the JCM 10874T strain., American Society for Microbiology
Genome Announcements, 2016, [Reviewed]

土壌には未知の脱窒微生物が豊富に存在している
磯部一夫; 魏魏; 西澤智康; 白鳥豊; 大塚重人; 妹尾啓史
土と微生物, 01 Oct. 2015, [Reviewed]

土壌生成プロセスにおける微生物の役割
太田寬行; 西澤智康; 藤村玲子, 日本土壌微生物学会
土と微生物, Oct. 2015, [Invited]

Higher diversity and abundance of denitrifying microorganisms in environments than considered previously
Wei Wei; Kazuo Isobe; Tomoyasu Nishizawa; Lin Zhu; Yutaka Shiratori; Nobuhito Ohte; Keisuke Koba; Shigeto Otsuka; Keishi Senoo, Denitrification is an important process in the global nitrogen cycle. The genes encoding NirK and NirS (nirK and nirS), which catalyze the reduction of nitrite to nitric oxide, have been used as marker genes to study the ecological behavior of denitrifiers in environments. However, conventional polymerase chain reaction (PCR) primers can only detect a limited range of the phylogenetically diverse nirK and nirS. Thus, we developed new PCR primers covering the diverse nirK and nirS. Clone library and qPCR analysis using the primers showed that nirK and nirS in terrestrial environments are more phylogenetically diverse and 2-6 times more abundant than those revealed with the conventional primers. RNA-and culture-based analyses using a cropland soil also suggested that microorganisms with previously unconsidered nirK or nirS are responsible for denitrification in the soil. PCR techniques still have a greater capacity for the deep analysis of target genes than PCR-independent methods including metagenome analysis, although efforts are needed to minimize the PCR biases. The methodology and the insights obtained here should allow us to achieve a more precise understanding of the ecological behavior of denitrifiers and facilitate more precise estimate of denitrification in environments., NATURE PUBLISHING GROUP
ISME JOURNAL, Sep. 2015, [Reviewed]

Development of PCR primers targeting fungal nirK to study fungal denitrification in the environment
Wei Wei; Kazuo Isobe; Yutaka Shiratori; Tomoyasu Nishizawa; Nobuhito Ohte; Yuta Ise; Shigeto Otsuka; Keishi Senoo, Fungal denitrification in soils is receiving considerable attention as one of the dominant N2O production processes. However, because of the lack of a methodology to detect fungal denitrification-related genes, the diversity and ecological behavior of denitrifying fungi in soil remains unknown. Thus, we designed a primer set to detect the fungal nitrite reductase gene (nirK) and validated its sensitivity and specificity. Through clone library analyses, we identified congruence between phylogenies of the 18S rRNA gene and nirK of denitrifying fungal isolates obtained from the surface-fertilized cropland soil and showed that fungi belonging to Eurotiales, Hypocreales, and Sordariales were primarily responsible for N2O emissions in the soil. (C) 2014 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
SOIL BIOLOGY & BIOCHEMISTRY, Feb. 2015, [Reviewed]

Molecular analysis of the cyanobacterial community in gastric contents of egrets with symptoms of steatitis
Tomoyasu Nishizawa; Yasuko Neagari; Takamasa Miura; Munehiko Asayama; Koichi Murata; Ken-Ichi Harada; Makoto Shirai, Many deaths of wild birds that have drunk water contaminated with hepatotoxic microcystin-producing cyanobacteria have been reported. A mass death of egrets and herons with steatitis were found at the agricultural reservoir occurring cyanobacterial waterblooms. This study aimed to verify a hypothesis that the egrets and herons which died in the reservoir drink microcystin-producing cyanobacteria and microcystin involves in the cause of death as well as the symptoms of steatitis. The cyanobacterial community in gastric contents of egrets and herons that died from steatitis was assessed using cyanobacterial 16S rRNA-based terminal-restriction fragment length polymorphism (T-RFLP) profiling and a cyanobacterial 16S rRNA-based clone library analysis. In addition, PCR amplification of the mcyB–C region and the mcyG gene, involved in microcystin biosynthesis, was examined. The cyanobacterial community in the gastric contents of two birds showed a simplistic composition. A comparison of cyanobacterial T-RFLP profiling and cloned sequences suggested that the genus Microcystis predominated in both samples of egrets died. Although we confirmed that two egrets which died in the reservoir have taken in cyanobacterial waterblooms containing the genus Microcystis, no mcy gene was detected in both samples according to the mcy gene-based PCR analysis. This study is the first to show the profiling and traceability of a cyanobacterial community in the gastric contents of wild birds by molecular analysis. Additionally, we consider causing symptoms of steatitis in the dead egrets., Bentham Science Publishers B.V.
Open Microbiology Journal, 2015, [Reviewed]

Change in rhizosphere microbial community of Miscanthus condensatus, a pioneer plant on recent Miyake-jima volcanic deposit, during the vegetation development(The Joint Meeting of Japanese Environmental Microbiology-related Associations 2014)　　　　　　　　　　　　　　　
Guo,Yong; Fujimura,Reiko; Sato,Yoshinori; Nishizawa,Tomoyasu; Kamijo,Takashi; Ohta,Hiroyuki, the Japanese Society of Soil Microbiology
土と微生物, Oct. 2014

微生物の遺伝子解析からゲノム解析、そしてゲノム情報活用へ
西澤智康, Lead, 日本土壌微生物学会
土と微生物, Oct. 2014

Construction of a stepwise gene integration system by transient expression of actinophage R4 integrase in cyanobacterium Synechocystis sp PCC 6803
Takamasa Miura; Akito Nishizawa; Tomoyasu Nishizawa; Munehiko Asayama; Hideo Takahashi; Makoto Shirai, The integrase of actinophage R4, which belongs to the large serine-recombinase family, catalyzes site-specific recombination between two distinct attachment site sequences of the phage (attP) and actinomycete Streptomyces parvulus 2297 chromosome (attB). We previously reported that R4 integrase (Sre) catalyzed site-specific recombination both in vivo and in vitro. In the present study, a Sre-based system was developed for the stepwise site-specific integration of multiple genes into the chromosome of cyanobacterium Synechocystis sp. PCC 6803 (hereafter PCC 6803). A transgene-integrated plasmid with two attP sites and a non-replicative sre-containing plasmid were co-introduced into attB-inserted PCC 6803 cells. The transiently expressed Sre catalyzed highly efficient site-specific integration between one of the two attP sites on the integration plasmid and the attB site on the chromosome of PCC 6803. A second transgene-integrated plasmid with an attB site was integrated into the residual attP site on the chromosome by repeating site-specific recombination. The transformation frequencies (%) of the first and second integrations were approximately 5.1 x 10(-5) and 8.2 x 10(-5), respectively. Furthermore, the expression of two transgenes was detected. This study is the first to apply the multiple gene site-specific integration system based on R4 integrase to cyanobacteria., SPRINGER HEIDELBERG
MOLECULAR GENETICS AND GENOMICS, Aug. 2014, [Reviewed]

Inoculation with N-2-generating denitrifier strains mitigates N2O emission from agricultural soil fertilized with poultry manure
Tomoyasu Nishizawa; Aihua Quan; Ayaaki Kai; Kanako Tago; Satoshi Ishii; Weishou Shen; Kazuo Isobe; Shigeto Otsuka; Keishi Senoo, Pelleted poultry manure is recommended for use with agricultural soil as a replacement for chemical fertilizers; however, application of the manure stimulates nitrous oxide (N2O) emission from the soil through denitrification. To mitigate the N2O emission caused by application of pelleted poultry manure, soil microcosms were set up; each microcosm was inoculated with one of the following N-2-generating denitrifier strains previously been isolated from paddy soil: Azoarcus, Dyella, Dechloromonas, Niastella, and Burkholderia. When pelleted poultry manure was incubated on its own, N2O was produced by denitrification. In contrast, N2O emission was significantly lowered when the manure was inoculated with most of the N-2-generating strains. In soil microcosms, N2O was emitted during incubation after application of the pelleted manure, while N2O flux was significantly lowered when the soil was inoculated with Azoarcus sp. KS11B, Niastella sp. KS31B, or Burkholderia sp. TSO47-3 on the 12th day of incubation. In addition, when pelleted manure was inoculated with the strains prior to application in the soil microcosms, the level of N2O emission was significantly lowered to ca. 40-60 % that from the non-inoculated control. Our study provides the prototype of a technique that uses microbial technology to mitigate N2O emission from agricultural soil fertilized with pelleted poultry manure., SPRINGER
BIOLOGY AND FERTILITY OF SOILS, Aug. 2014, [Reviewed]

Plant growth promoting ability of Azoarcus sp. KH32C, a N₂O-mitigating denitrifier isolated from rice paddy soil　　　　　　　　　　　　　　　
Nishizawa; T.; T. Hakoyama; S. Uraguchi; H. Kakuta; K. Isobe; S. Otsuka; S. Sakuda; T. Fujiwara; K. Senoo, Lead, EAFES
The 6th EAFES International Congress, Apr. 2014

Root-associated microbial diversity of a pioneer grass, Miscanthus condensatus, on the recent volcanic deposits in the Island of Miyake, Japan　　　　　　　　　　　　　　　
Guo; Y.; R. Fujimura; Y. Sato; T. Nishizawa; T. Kamijo; H. Ohta, EAFES
The 6th EAFES International Congress, Apr. 2014

N2O emission from cropland field soil through fungal denitrification after surface applications of organic fertilizer
Wei Wei; Kazuo Isobe; Yutaka Shiratori; Tomoyasu Nishizawa; Nobuhito Ohte; Shigeto Otsuka; Keishi Senoo, Agricultural fields are one of the largest anthropogenic sources of atmospheric nitrous oxide (N2O). Fungi have been suggested to contribute substantially to N2O emission in terrestrial environments; however, the extent of the fungal contribution and the phylogenetic and physiologic nature of related fungal communities in agricultural fields are largely unknown. This study focused on the large N2O emission from cropland soil that occurs after granular organic fertilizers were applied to the surface. The granular organic fertilizers applied were evidently covered by fungal mycelia. An experiment using a soil microcosm was established to imitate the field observations. N2O emissions following surface organic fertilization were suppressed by 84 and 20% after the addition of cycloheximide (a fungal inhibitor) and streptomycin (a bacterial inhibitor), respectively, suggesting that fungi provide the main contribution to the observed N2O emission. The population density and community composition of fungi in the surface-fertilized and non-fertilized soils in the field were determined using colony counting, denaturing gradient gel electrophoresis and subsequent phylogenetic analyses. Thirty-four fungal strains were isolated from the soils, and their N2O producing activities were analyzed. Fungal population density in the surface-fertilized soil (2.6 x 10(6) CFU/g) was much higher than that in the non-fertilized soil (1.0 x 105 CFU/g). In addition, the fungal community compositions of the soils differed. Actinomucor elegans, Bionectria ochroleuca, Fusarium avenaceum, Fusarium equiseti, Fusarium oxysporum, Fusarium solani and Nectria sp. dominated the surface-fertilized soil, and their activity in producing N2O was confirmed. These results suggested that N2O emission after the surface application of granular organic fertilizers in the cropland field mainly resulted from fungal denitrification. (C) 2013 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
SOIL BIOLOGY & BIOCHEMISTRY, Feb. 2014, [Reviewed]

Draft genome sequence of the betaproteobacterial endosymbiont associated with the fungus Mortierella elongata FMR23-6
Reiko Fujimura; Ayumu Nishimura; Shoko Ohshima; Yoshinori Sato; Tomoyasu Nishizawa; Kenshiro Oshima; Masahira Hattori; Kazuhiko Narisawa; Hiroyuki Ohta, The fungus Mortierella elongata FMR23-6 harbors an endobacterium inside its mycelium. Attempts to isolate the endobacterium from the fungus were not yet successful, but a highly purified bacterial fraction was prepared. Here, we report the draft genome sequence of the endobacterium., American Society for Microbiology
Genome Announcements, 2014, [Reviewed]

Characterization of lysis of the multicellular cyanobacterium Limnothrix/Pseudanabaena sp. strain ABRG5-3
Kitazaki; C.; S. Numano; A. Takanezawa; T. Nishizawa; M. Shirai; M. Asayama, The cyanobacterium semi-filamentous multicellular strain ABRG5-3 undergoes cell lysis as a unique feature that occurs due to growth condition changes from normal cultivation with shaking to static cultivation without shaking in liquid culture (Nishizawa et al., 2010). Microscopic observation and energy dispersive X-ray spectrometer (EDX) analysis have revealed that lysis is involved in the accumulation of polyphosphate compounds and the disintegration of thylakoid membranes in cells. Static cultivation, dark or red light exposure, and temperature (22 to 42 degrees C) conditions were found to be effective factors for the induction of lysis. Moreover, stress induced by salts, osmotic pressure with sucrose, and the depletion of nitrogen or phosphate in cultures also induced ABRG5-3 cell lysis. Based on these results, we discuss lysis and its utilization in the biotechnology industry., Taylor & Francis
Biosci. Biotechnol. Biochem., Dec. 2013, [Reviewed]

Taxonomic composition of denitrifying bacterial isolates is different among three rice paddy field soils in Japan
Tomoyasu Nishizawa; Yusuke Uei; Kanako Tago; Kazuo Isobe; Shigeto Otsuka; Keishi Senoo, Denitrification is one of the important nitrogen transformation processes in rice (Oryza sativa L.) paddy soil. We have performed both culture-dependent and -independent studies to investigate the key players in denitrification in rice paddy soil. Our previous works suggested the possibility that community composition of denitrifying populations is different among soils. To confirm this possibility, in the present study, denitrifying bacteria were isolated from rice paddy field soils (Kumamoto and Tokyo) in Japan by the functional single-cell isolation method, and examined for their taxonomic position. Combined with our previously isolated strains from Niigata paddy field soil, our results clearly indicated that the taxonomic composition of the denitrifying bacterial community is different at the genus level among three soils. A significant difference in mean potential denitrification activity of the isolates at the genus level was also found. Soil and/or other environmental factors could shape the community structure of denitrifiers in rice paddy soil., TAYLOR & FRANCIS LTD
SOIL SCIENCE AND PLANT NUTRITION, Jun. 2013, [Reviewed]

Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
Yoshinori Sato; Hiroyuki Ohta; Takao Yamagishi; Yong Guo; Tomoyasu Nishizawa; M. Habibur Rahman; Hisao Kuroda; Task Kato; Masanori Saito; Ikuo Yoshinaga; Kazuyuki Inubushi; Yuichi Suwa, An anammox assay involving a N-15 tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N-2 production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca. 'B. anammoxidans', and Ca. 'Kuenenia stuttgartiensis'. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Sep. 2012, [Reviewed]

Complete Genome Sequence of Leptospirillum ferrooxidans Strain C2-3, Isolated from a Fresh Volcanic Ash Deposit on the Island of Miyake, Japan
Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Kenshiro Oshima; Seok-Won Kim; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta, A diazotrophic, acidophilic, iron-oxidizing bacterium, Leptospirillum ferrooxidans, known to be difficult to cultivate, was isolated from a fresh volcanic ash deposit on the island of Miyake, Japan. Here, we report the complete genome sequence of a cultured strain, C2-3., AMER SOC MICROBIOLOGY
JOURNAL OF BACTERIOLOGY, Aug. 2012, [Reviewed]

Heterologous expression of a plant ReIA-SpoT homologue results in increased stress tolerance in Saccharomyces cerevisiae by accumulation of the bacterial alarmone ppGpp
Kozo Ochi; Tomoyasu Nishizawa; Takashi Inaoka; Akiyo Yamada; Kohsuke Hashimoto; Takeshi Hosaka; Susumu Okamoto; Yoshihiro Ozeki, The bacterial alarmone ppGpp is present only in bacteria and the chloroplasts of plants, but not in mammalian cells or eukaryotic micro-organisms such as yeasts and fungi. The importance of the ppGpp signalling system in eukaryotes has therefore been largely overlooked. Here, we demonstrated that heterologous expression of a reIA-spoT homologue (Sj-RSH) isolated from the halophilic plant Suaeda japonica in the yeast Saccharomyces cerevisiae results in accumulation of ppGpp, accompanied by enhancement of tolerance against various stress stimuli, such as osmotic stress, ethanol, hydrogen peroxide, high temperature and freezing. Unlike bacterial ppGpp accumulation, ppGpp was accumulated in the early growth phase but not in the late growth phase. Moreover, nutritional downshift resulted in a decrease in ppGpp level, suggesting that the observed Sj-RSH activity to synthesize ppGpp is not starvation-dependent, contrary to our expectations based on bacteria. Accumulated ppGpp was found to be present solely in the cytosolic fraction and not in the mitochondrial fraction, perhaps reflecting the ribosome-independent ppGpp synthesis in S. cerevisiae cells. Unlike bacterial inosine monophosphate (IMP) dehydrogenases, the IMP dehydrogenase of S. cerevisiae was insensitive to ppGpp. Microarray analysis showed that ppGpp accumulation gave rise to marked changes in gene expression, with both upregulation and downregulation, including changes in mitochondrial gene expression. The most prominent upregulation (38-fold) was detected in the hypothetical gene YBR072C-A of unknown function, followed by many other known stress-responsive genes. S. cerevisiae may provide new opportunities to uncover and analyse the ppGpp signalling system in eukaryotic cells., SOC GENERAL MICROBIOLOGY
MICROBIOLOGY-SGM, Aug. 2012, [Reviewed]

Complete Genome Sequence of the Denitrifying and N2O-Reducing Bacterium Azoarcus sp Strain KH32C
Tomoyasu Nishizawa; Kanako Tago; Kenshiro Oshima; Masahira Hattori; Satoshi Ishii; Shigeto Otsuka; Keishi Senoo, We report the finished and annotated genome sequence of a denitrifying and N2O-reducing betaproteobacterium, Azoarcus sp. strain KH32C. The genome is composed of one chromosome and one megaplasmid and contains genes for plant-microbe interactions and the gene clusters for aromatic-compound degradations., AMER SOC MICROBIOLOGY
JOURNAL OF BACTERIOLOGY, Mar. 2012, [Reviewed]

Analysis of Early Bacterial Communities on Volcanic Deposits on the Island of Miyake (Miyake-jima), Japan: a 6-year Study at a Fixed Site
Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Kenji Nanba; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta, Microbial colonization on new terrestrial substrates represents the initiation of new soil ecosystem formation. In this study, we analyzed early bacterial communities growing on volcanic ash deposits derived from the 2000 Mount Oyama eruption on the island of Miyake (Miyake-jima), Japan. A site was established in an unvegetated area near the summit and investigated over a 6-year period from 2003 to 2009. Collected samples were acidic (pH 3.0-3.6), did not utilize any organic substrates in ECO microplate assays (Biolog), and harbored around 10(6) cells (g dry weight)(-1) of autotrophic Fe(II) oxidizers by most-probable-number (MPN) counts. Acidithiobacillus ferrooxidans, Acidithiobacillus ferrivorans, and the Leptospirillum groups I, II and III were found to be abundant in the deposits by clone library analysis of bacterial 16S rRNA genes. The numerical dominance of Acidithiobacillus ferrooxidans was also supported by analysis of the gene coding for the large subunit of the form I ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). Comparing the 16S rRNA gene clone libraries from samples differing in age, shifts in Fe(II)-oxidizing populations seemed to occur with deposit aging. The detection of known 16S rRNA gene sequences from Fe(III)-reducing acidophiles promoted us to propose the acidity-driven iron cycle for the early microbial ecosystem on the deposit., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Mar. 2012, [Reviewed]

Advantages of functional single-cell isolation method over standard agar plate dilution method as a tool for studying denitrifying bacteria in rice paddy soil
Tomoyasu Nishizawa; Kanako Tago; Yusuke Uei; Satoshi Ishii; Kazuo Isobe; Shigeto Otsuka; Keishi Senoo, We recently established a method for isolating functional single cells from environmental samples using a micromanipulator (Functional single-cell (FSC) isolation), and applied it to the study of denitrifying bacteria in rice paddy soil (Ashida et al. 2010. Appl Microbiol Biotechnol 85:1211-1217). To further examine the advantages and possible disadvantages of the FSC method, we isolated denitrifying bacteria from the same rice paddy soil sample using both FSC and standard agar plate dilution (APD) methods and compared in this study. The proportion of denitrifying bacteria in the total isolates was more than 6-fold larger with FSC isolation (57.1%) compared with the APD method (9.2%). Denitrifying bacteria belonging to Alphaproteobacteria and Bacilli were commonly isolated using both methods, whereas those belonging to Betaproteobacteria, which had been found to be active in the denitrification-inductive paddy soil, were isolated only with the FSC method. On the other hand, Actinobacteria were only isolated using the APD method. The mean potential denitrification activity of the FSC isolates was higher than that of the APD isolates. Overall, FSC isolation was confirmed to be an excellent method for studying denitrifying bacteria compared with the standard agar plate dilution method., BIOMED CENTRAL LTD
AMB EXPRESS, 2012, [Reviewed]

Complete genome sequence of the denitrifying and N₂O-reducing bacterium Pseudogulbenkiania sp. strain NH8B
Ishii; S.; K. Tago; T. Nishizawa; K. Oshima; M. Hattori; K. Senoo, Pseudogulbenkiania sp. strain NH8B is a Neisseriales bacterium isolated from an agricultural field. This strain has strong denitrification and N2O reduction activities. Here, we report the finished and annotated genome sequence of this organism., Am Soc Microbiol
J. Bacteriol., Oct. 2011, [Reviewed]

In vivo and in vitro characterization of site-specific recombination of actinophage R4 integrase
Miura; T.; Y. Hosaka; Y.-Z. Yang; T. Nishizawa; M. Asayama; H. Takahashi; and M. Shirai, The site-specific integrase of actinophage R4 belongs to the serine recombinase family. During the lysogenization process, it catalyzes site-specific recombination between the phage genome and the chromosome of Streptomyces parvulus 2297. An in vivo assay using Escherichia coli cells revealed that the minimum lengths of the recombination sites attB and attP are 50-bp and 49-bp, respectively, for efficient intramolecular recombination. The in vitro assay using overproduced R4 integrases as a hexahistidine (His(6))-glutathione-S-transferase (GST)-R4 integrase fusion protein, showed that the purified protein preparation retains the site-specific recombination activity which catalyzes the site-specific recombination between affP and attB in the intermolecular reaction. It also revealed that the inverted repeat within attP is essential for efficient in vitro intermolecular recombination. In addition, a gel shift assay showed that His(6)-GST-R4 integrase bound to the 50-bp attB and 49-bp attP specifically. Moreover, based on a detailed comparison analysis of amino acid sequences of serine integrases, we found the DNA binding region that is conserved in the serine recombinase containing the large C-terminal domain. Based on the results presented on this report, attachment sites needed in vitro and in vivo for site-specific recombination by the R4 integrase have been defined more precisely. This knowledge is useful for developing new genetic manipulation tools in the future., Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
J. Gen. Appl. Microbiol., Apr. 2011, [Reviewed]

Phylogenetic and Functional Diversity of Denitrifying Bacteria Isolated from Various Rice Paddy and Rice-Soybean Rotation Fields
Kanako Tago; Satoshi Ishii; Tomoyasu Nishizawa; Shigeto Otsuka; Keishi Senoo, Denitrifiers can produce and consume nitrous oxide (N(2)O). While little N(2)O is emitted from rice paddy soil, the same soil produces N(2)O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had been used for continuous cultivation of rice and the other for rotational cultivation of rice and soybean. Active denitrifiers were isolated from these soils using a functional single-cell isolation method, and their taxonomy and denitrifying properties were examined. A total of 110 denitrifiers were obtained, including those previously detected by a culture-independent analysis. Strains belonging to the genus Pseudogulbenkiania were dominant at all locations, suggesting that Pseudogulbenkiania denitrifiers are ubiquitous in various rice paddy soils. Potential denitrifying activity was similar among the strains, regardless of the differences in taxonomic position and soil of origin. However, relative amounts of N(2) in denitrification end products varied among strains isolated from different locations. Our results also showed that crop rotation had minimal impact on the functional diversity of the denitrifying strains. These results indicate that soil and other environmental factors, excluding cropping systems, could select for N(2)-producing denitrifiers., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Mar. 2011, [Reviewed]

Limnobacter litoralis sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from a volcanic deposit, and emended description of the genus Limnobacter
Hongsheng Lu; Yoshinori Sato; Reiko Fujimura; Tomoyasu Nishizawa; Takashi Kamijo; Hiroyuki Ohta, A Gram-negative, aerobic, heterotrophic bacterium, designated KP1-19^T, was isolated from a 22-year-old volcanic deposit at a site lacking vegetation on the island of Miyake, Japan. Strain KP1-19^Twas able to use thiosulfate (optimum concentration 10 mM) as an additional energy source. 16S rRNA gene sequence analysis indicated that strain KP1-19^Twas closely related toLimnobacter thiooxidansCS-K2^Twithin the classBetaproteobacteria(97.7 % 16S rRNA gene sequence similarity). The cellular fatty acid profile was characteristic of the genusLimnobacter: the major fatty acids (>5 %) were C_16 : 0, C_16 : 1ω7cand C_18 : 1ω7cand minor amounts of C_10 : 03-OH were also found. DNA–DNA relatedness between strain KP1-19^TandL. thiooxidansLMG 19593^Twas 18 %. Therefore, strain KP1-19^Trepresents a novel species, for which the nameLimnobacter litoralissp. nov. is proposed. The type strain is KP1-19^T(=LMG 24869^T=NBRC 105857^T=CIP 109929^T)., Microbiology Society
International Journal of Systematic and Evolutionary Microbiology, 01 Feb. 2011, [Reviewed]

Characterization of the locus of genes encoding enzymes producing heptadepsipeptide micropeptin in the unicellular cyanobacterium Microcystis
Tomoyasu Nishizawa; Akiko Ueda; Tomoyo Nakano; Akito Nishizawa; Takamasa Miura; Munehiko Asayama; Kiyonaga Fujii; Ken-ichi Harada; Makoto Shirai, The gene cluster involved in producing the cyclic heptadepsipeptide micropeptin was cloned from the genome of the unicellular cyanobacterium Microcystis aeruginosa K-139. Sequencing revealed four genes encoding non-ribosomal peptide synthetases (NRPSs) that are highly similar to the gene cluster involved in cyanopeptolins biosynthesis. According to predictions based on the non-ribosomal consensus code, the order of the mcnABCE NPRS modules was well consistent with that of the biosynthetic assembly of cyclic peptides. The biochemical analysis of a McnB(K-139) adenylation domain and the knock-out of mcnC in a micropeptin-producing strain, M. viridis S-70, revealed that the mcn gene clusters were responsible for the production of heptadepsipeptide micropeptins. A detailed comparison of nucleotide sequences also showed that the regions between the mcnC and mcnE genes of M. aeruginosa K-139 retained short stretches of DNA homologous to halogenase genes involved in the synthesis of halogenated cyclic peptides of the cyanopeptolin class including anabaenopeptilides. This suggests that the mcn clusters of M. aeruginosa K-139 have lost the halogenase genes during evolution. Finally, a comparative bioinformatics analysis of the congenial gene cluster for depsipetide biosynthesis suggested the diversification and propagation of the NRPS genes in cyanobacteria., OXFORD UNIV PRESS
JOURNAL OF BIOCHEMISTRY, 2011, [Reviewed]

Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata
Yoshinori Sato; Kazuhiko Narisawa; Kazuto Tsuruta; Masafumi Umezu; Tomoyasu Nishizawa; Kenji Tanaka; Kaoru Yamaguchi; Masakazu Komatsuzaki; Hiroyuki Ohta, Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N(2)O-producing activity and the presence of endobacteria., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Dec. 2010, [Reviewed]

細菌の生態学的種を考える
西澤智康; 佐藤嘉則; 藤村玲子; 太田寛行, Lead, 16S rRNA 遺伝子解析が導入される前の時代，土壌細菌研究者の多くは，分離菌株の分類学的位置づけがどのような生態的意味をもつかについて一度は考え悩むときがあったと思う．そのような時代の最初の頃に，栗原は「ecological species(生態学的種)の概念の確立」を説いた(1965年)．2000年代に入り，遺伝子変異の観点から細菌のecotype(エコタイプ)や細菌種の見直しが盛んに議論されている．ここでは，まず栗原の考察を読み解き，Cohanが提唱する細菌の「エコタイプ」について解説するとともに，著者らが扱ってきた初成土壌(三宅島2000年噴火火山灰堆積物)に住みつく細菌の分析結果を紹介して，細菌の生態学的種について考える話題を提供する．, 日本農芸化学会
化学と生物, Oct. 2010

Molecular Characterization of Fungal Communities in Non-Tilled, Cover-Cropped Upland Rice Field Soils
Tomoyasu Nishizawa; Zhaorigetu; Masakazu Komatsuzaki; Yoshinori Sato; Nobuhiro Kaneko; Hiroyuki Ohta, This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth years were analyzed using T-RFLP (terminal restriction fragment length polymorphism) profiling and clone libraries with the internal transcribed spacer (ITS) region and domain 1 (D1) of the fungal large-subunit (fLSU) rRNA (D1(fLSU)) as the target DNA sequence. From the 2nd-year-sample, 372 cloned sequences of fungal ITS-D1(fLSU) were obtained and clustered into 80 non-redundant fungal OTUs (operational taxonomic units) in 4 fungal phyla. The T-RFLP profiling was performed with the 2nd- and 5th-year-samples and the major T-RFs (terminal restriction fragments) were identified using a theoretical fragment analysis of the ITS-D1(fLSU) clones. These molecular analyses showed that the fungal community was influenced more strongly by the cover-cropping than tillage practices. Moreover, the non-tilled, cover-cropped soil was characterized by a predominance of Cryptococcus sp. in the phylum Basidiomycota. We provided a genetic database of the fungal ITS-D1(fLSU)s in the differently managed soils of upland rice fields., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, Sep. 2010, [Reviewed]

Isolation and Molecular Characterization of a Multicellular Cyanobacterium, Limnothrix/Pseudanabaena sp Strain ABRG5-3
Tomoyasu Nishizawa; Tomoyo Hanami; Eriko Hirano; Takamasa Miura; Yuko Watanabe; Akira Takanezawa; Masakazu Komatsuzaki; Hiroyuki Ohta; Makoto Shirai; Munehiko Asayama, A cyanobacterium, semi-filamentous multicellular strain ABRG5-3, was isolated and its unique nature was characterized. This axenic strain formal colonies and was motile on an agarose plate. The 16S rRNA gene of ABRG5-3 exhibited similarities to those of the Limnothrix and Pseudanabaena strains, which are known as filamentous and nonheterocystous cyanobacteria. Peaks in absorbance for the accumulation of chlorophyll a, phycocyanin, and phycoerythrin were observed in the cell extract. Natural separation of the pigments occurred in the supernatant of the autolysed cells. The cell lysis was promoted by osmotic shocks and lysozyme treatments. Chlorophyll a and total DNA were abundantly recovered from the cells. Analysis of the restriction-modification system for genomic DNA revealed novel diversity. Moreover, we made a successful attempt to create antibiotic-resistant strains by conjugation with a foreign plasmid, which indicates that strain ABRG5-3 is transformable., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2010, [Reviewed]

末端制限断片（T-RFs）プロファイル情報に基づく土壌微生物群集構造解析
西澤智康; 小松﨑将一; 金子信博; 太田寛行, Lead, 土壌環境中に存在する微生物の多様性や土壌環境変化への微生物群集の応答を調べるため,リアルタイム定量PCRアッセイと末端制限断片長多型解析(T-RFLP)法を併用した手法を確立した。この解析手法から,標的遺伝子領域に基づく微生物群の末端制限断片(T-RFs)サイズとピーク高(相対値)の情報が得られる。T-RFLPプロファイリングの実測情報とクローン解析から得られた遺伝子配列のin silico解析によるT-RFsプロファイル情報がほぼ一致することから,本法が精度の高い解析技術であることを示した。また,検出されたT-FRsプロファイル情報をもとに多次元尺度構成法分析で微生物群集の類似性とその動態を表す方法,そしてT-RFsの相対的存在量の頻度分布順位図から微生物の多様性を示す方法を紹介した。さらに,T-RFsプロファイル情報をもとに土壌微生物群集を推定するバイオインフォマテッィクスの利用方法を示した。今後のデータベースの充実により,定量PCR-T-KELP法が土壌微生物群集モニタリングに大いに役立つことを期待している。, 日本土壌微生物学会
土と微生物, Apr. 2010

Correlation between variation in McyB1 module and biosynthesis of microcystin isoforms
Nishizawa; A.; T. Nishizawa; M. Asayama; M. Hasegawa; K. Harada; M. Shirai, Res Insti Meijo Univ
J. Res. Insti. Meijo Univ., Dec. 2009, [Reviewed]

Genetic analysis of the microcystin biosynthesis gene cluster in Microcystis strains from four bodies of eutrophic water in Japan
Noguchi; T.; A. Shinohara; A. Nishizawa; M. Asayama; T. Nakano; M. Hasegawa; K. Harada; T. Nishizawa; M. Shirai, The highly conserved organization of microcystin biosynthesis (mcy) gene clusters, which includes nonribosomal peptide synthetase (NRPS) genes, polyketide synthase (PKS) genes, and fused NRPS-PKS genes, has been characterized in the genus Microcystis. In this study, a total of 135 cyanobacterial strains from four different geographical locations in Japan were isolated. Fourteen mcy-possessing (mcy(+)) strains were identified according to PCR amplification between two genes from domestic mcy(+) strains and the mcy gene's organization was classified into five types. Phylogenetic relationships of the 16S-23S internal transcribed spacer region indicated that the five types of mcy gene cluster structure classified into two groups of the genus Microcystis. HPLC of the isolated mcy(+) strain containing a partial deletion of mcyI (Delta mcyI) revealed that microcystin production disappeared. A transcriptional analysis of the Delta mcyI-strain and an assay of recombinant McyI dehydrogenase activity showed that McyI is responsible for microcystin biosynthesis. Based on patterns of the PCR amplicons and analyses of nucleotide sequences in the mcy gene cluster of Microcystis, we confirmed the presence of inserts at three specific loci, between mcyA and mcyD, and downstream of mcyC and mcyI. Our study is the first investigation of the mcy gene cluster structure in the genus Microcystis from environmental samples., Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
J. Gen. Appl. Microbiol., Apr. 2009, [Reviewed]

Nitrogenase Activity (Acetylene Reduction) of an Iron-Oxidizing Leptospirillum Strain Cultured as a Pioneer Microbe from a Recent Volcanic Deposit on Miyake-Jima, Japan
Yoshinori Sato; Ken Hosokawa; Reiko Fujimura; Tomoyasu Nishizawa; Takashi Kamijo; Hiroyuki Ohta, The genus Leptospirillum is known to dominate acid mine drainage and bioleaching systems. In this paper, we describe the isolation of iron-oxidizing bacteria closely related to Leptospirillum ferrooxidans from an acidic volcanic ash deposit on the island of Miyake (Miyake-jima), Japan. We further show the nitrogenase activity (acetylene reduction) for one (strain C2-1) of the isolates. The deposit harbored 1.2 x 10(8) total direct count (g dry weight)(-1) and 7.1 x 10(5) (most-probable-number, MPN) (g dry weight)(-1) of iron-oxidizer. A ferrous iron-limited, aerobic chemostat culture using Leptospirillum HH medium (pH 1.8) was performed to select and isolate the Leptospirillum group. Nine isolates were regarded as pure cultures based on uniform colony morphology on Fe(2+)-containing silica gel plates and absence of growth on 100-fold diluted nutrient broth plates. Six strains examined further shared 99.9-100% identity in 16S rRNA gene sequence with each other. Homology-based searches showed that all of the strains belonged to the Leptospirillum ferrooxidans clade. Strain C2-1 grown in ammonium sulfate-free Leptospirillum HH medium (pH 1.8) showed an initial rate of acetylene reduction of 58 mu mol h(-1) (g cell carbon)(-1)., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, 2009, [Reviewed]

Archaeal Diversity of Upland Rice Field Soils Assessed by the Terminal Restriction Fragment Length Polymorphism Method Combined with Real Time Quantitative-PCR and a Clone Library Analysis
Tomoyasu Nishizawa; Masakazu Komatsuzaki; Nobuhiro Kaneko; Hiroyuki Ohta, The PCR amplification-based analysis of microbial diversity is subject to potential problems. In this study, to minimize the bias toward a 1: 1 ratio in multitemplate PCR, a real-time PCR assay was carried out using a quenching fluorescence dye primer and amplification efficiency was monitored. Then terminal-restriction fragment length polymorphism (T-RFLP) profiling was performed using the PCR product with minimized PCR bias. This method was applied to an analysis of the diversity of the archacal community in an upland rice field under different tillage systems and winter cover cropping. Terminal restriction fragments (T-RFs) of PCR-amplified archacal 16S rRNA genes were assigned to the gene sequences recovered from the same soil by using an archaeal 16S rRNA gene clone library. Our results indicated that soil archaeal members were not influenced but the relative abundance of archaeal species particularly those belonging to Crenarchaeota which changed between the tillage and non-tillage treatments., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
MICROBES AND ENVIRONMENTS, 2008, [Reviewed]

Diversity within the microcystin biosynthetic gene clusters among the genus Microcystis
Nishizawa; T.; A. Nishizawa; M. Asayama; K. Harada; M. Shirai, Lead, The principle organization of microcystin biosynthesis (mcy) gene clusters is characterized in three genera among the microcystin-producing cyanobacteria. To examine the conservation of the mcy gene clusters among the genus Microcystis, internal non-coding regions of the cluster in 21 strains including M. aeruginosa, M. viridis, and M. wesenbergii, were amplified by PCR using specific primer sets and sequenced. PCR-based analysis revealed a completely conserved organization of the bidirectional cluster structure, mcyABC and mcyDEFGHIJ, among all toxic and some microcystin-negative strains. Furthermore, all mcy gene clusters were flanked by a gene with homology to dnaN adjacent to mcyJ and by umal with no-homology to any known genes at the 3'-end of mcyC. Transposase homolog genes were observed in a non-coding region between dnaN and mcyJ in three rnicrocystin-producing Microcystis strains. Sequence analysis of a region adjacent to umal in mcy-negative strains revealed two types of gene constitution, dnaN-umal and the hypothetical protein gene-umal. PCR-based analyses can be used to assess the identification of microcystin-producer strains in Microcystis spp., Jap Soc Microb Eco & Jap Soc Soil Microbiol
Microbes Environ., Dec. 2007, [Reviewed]

Cloning and characterization of a new hetero-gene cluster of nonribosomal synthetase and polyketide synthase from the cyanobacterium Microcystis aeruginosa K-139
Nishizawa; A.; A.B. Arshad; T. Nishizawa; M. Asayama; K. Fujii; T. Nakano; K. Harada; M. Shirai, Two nonribosomal peptide synthetase genes responsible for the biosynthesis of microcystin and micropeptin in Microcystis aeruginosa K-139 have been identified. A new nonribosomal peptide synthetase gene, psm3, was identified in M. aeruginosa K-139. The gene is a cluster extending 30 kb and comprising 13 bidirectionally transcribed open reading frames arranged in two putative operons. psm3 encodes four adenylation proteins, one polyketide synthase, and several unique proteins, especially Psm3L consisting of halogenase, acyl-CoA binding protein-like protein, and acyl carrier protein. Alignment of the binding pocket of the adenylation domain and an ATIR-PPi exchange analysis using a recombinant protein with the adenylation domain of Psm3B showed that Psm3G and Psm3B activate aspartic acid and tyrosine, respectively. Although disruption of psm3 did not reveal the product produced by Psm3, we identified microviridin B and aeruginosin K139 in the cells of M. aeruginosa K-139. The above-mentioned results indicated that M. aeruginosa possesses at least five nonribosomal peptide synthetase gene clusters., Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
J. Gen. Appl. Microbiol., Apr. 2007, [Reviewed]

トキシンをつくるシアノバクテリア
西澤智康; 白井 誠, Lead, エヌ・ティー・エス
生物の科学 遺伝, Nov. 2006

Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus
Koji Kasai; Tomoyasu Nishizawa; Kosaku Takahashi; Takeshi Hosaka; Hiroyuki Aoki; Kozo Ochi, Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by ppGpp in the thermophillic bacterium Thermus thermophilus. Here we investigated the physiological basis of the stringent response by comparing the changes in intracellular ppGpp levels and the rate of RNA synthesis in stringent (rel(+); wild type) and relaxed (relA and relC; mutant) strains of T. thermophilus. We found that in wild-type T. thermophilus, as in other bacteria, serine hydroxamate, an amino acid analogue that inhibits tRNA(ser) aminoacylation, elicited a stringent response characterized in part by intracellular accumulation of ppGpp and that this response was completely blocked in a relA-null mutant and partially blocked in a relC mutant harboring a mutation in the ribosomal protein L11. Subsequent in vitro assays using ribosomes isolated from wild-type and relA and relC mutant strains confirmed that (p)ppGpp is synthesized by ribosomes and that mutation of RelA or L11 blocks that activity. This conclusion was further confirmed in vitro by demonstrating that thiostrepton or tetracycline inhibits (p)ppGpp synthesis. In an in vitro system, (p)ppGpp acted by inhibiting RNA polymerase-catalyzed 23S/5S rRNA gene transcription but at a concentration much higher than that of the observed intracellular ppGpp pool size. On the other hand, changes in the rRNA gene promoter activity tightly correlated with changes in the GTP but not ATP concentration. Also, (p)ppGpp exerted a potent inhibitory effect on IMP dehydrogenase activity. The present data thus complement the earlier structural analysis by providing physiological evidence that T. thermophilus does produce ppGpp in response to amino acid starvation in a ribosome-dependent (i.e., RelA-dependent) manner. However, it appears that in T. thermophilus, rRNA promoter activity is controlled directly by the GTP pool size, which is modulated by ppGpp via inhibition of IMP dehydrogenase activity. Thus, unlike the case of Escherichia coli, ppGpp may not inhibit T. thermophilus RNA polymerase activity directly in vivo, as recently proposed for Bacillus subtilis rRNA transcription (L. Krasny and R. L. Gourse, EMBO J. 23:4473-4483, 2004)., AMER SOC MICROBIOLOGY
JOURNAL OF BACTERIOLOGY, Oct. 2006, [Reviewed]

Enzymatic assembly of the bis-indole core of rebeccamycin
T Nishizawa; S Gruschow; DHE Jayamaha; C Nishizawa-Harada; DH Sherman, AMER CHEMICAL SOC
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Jan. 2006, [Reviewed]

Molecular analysis of the rebeccamycin L-amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243
T Nishizawa; CC Aldrich; DH Sherman, Rebeccamycin, a member of the tryptophan-derived indolocarbazole family, is produced by Lechevalieria aerocolonigenes ATCC 39243. The biosynthetic pathway that specifies biosynthesis of this important metabolite is comprised of 11 genes spanning 18 kb of DNA. A presumed early enzyme involved in elaboration of the rebeccamycin aglycone is encoded by rebO, located at the left-hand region of the reb gene cluster. The deduced protein product, RebO (51.9 kDa), is an L-amino acid oxidase (L-AAO) that has 27% identity to an L-AAO from Scomber japonicus (animal, mackerel) and is a member of the family of FAD-dependent oxidase enzymes. In order to study the biochemical properties of this key enzyme, the rebO gene was overexpressed and purified from Escherichia coli. Biochemical characterization showed that RebO is dimeric, with a molecular mass of approximately 101 kDa. Further analysis revealed that the enzyme contains a noncovalently bound FAD cofactor and is reoxidized at the expense of molecular oxygen by producing one molecule of hydrogen peroxide. Based on kinetic studies, RebO shows significant preference for 7-chloro-L-tryptophan, suggesting its likely role as the natural early pathway substrate. Furthermore, the native RebO enzyme has evident, albeit limited, flexibility as shown by bioconversion studies with unnatural substrates. This work provides the first analysis of a structural enzyme involved in construction of this important class of indolocarbazole natural products., AMER SOC MICROBIOLOGY
JOURNAL OF BACTERIOLOGY, Mar. 2005, [Reviewed]

DNA cloning of peptide synthetase from cyanobacterium Aphanizomenon flos-aquae
Masaaki Kodama; Jeen Hee Lee; Shigeharu Kinoshita; Shigeru Sato; Takehiko Ogata; Tomoyasu Nishizawa; Makoto Shirai; Yuzuru Shimizu; Shugo Watabe, SPRINGER TOKYO
FISHERIES SCIENCE, 2002

Cyclic heptapeptide microcystin bisosynthesis requires the glutamate racemase gene
Nishizawa; T.; M. Asayama; M. Shirai, Lead, It was demonstrated previously that the operon consisting of the nonribosomal peptide synthetase (NRPS) gene coupled with the polyketide synthase (PKS) gene involved in cyclic heptapeptide microcystin synthesis includes two different D-amino acid synthetase genes, an epimerization domain at the 3' end of module 2, and the racemase gene mcyF, To determine the role of mcyF in microcystin synthesis, gene-disruption and complementation analyses were carried out. Insertional mutagenesis in the mcyF gene, generated by homologous recombination, abolished only microcystin synthesis, but did not influence cell growth, Furthermore, McyF supported D-Glu-independent growth of a strain of Escherichia coli defective in D-Glu synthesis, It is concluded that mcyF is the glutamic acid racemase gene involved in the synthesis of D-Glu residues in the microcystin molecule. This is the first report of the racemase in prokaryotic NRPS., Microbiology Society
Microbiology, May 2001, [Reviewed]

解明されたアオコ毒素合成系：ポリケチド合成モジュールが融合した非リボソーム型ペプチド合成系
西澤智康; 白井 誠, Lead, 日本農芸化学会
化学と生物, Nov. 2000

Studies on biosynthesis of secondary metabolites produced by toxic cyanobacteria. (I). Structures and synthetase genes of toxic and non-toxic peptides.
Fujii; K.; T. Nishizawa; M. Asayama; K. Sivonenn; M. Shirai; K. Harada, The occurrence of water blooms of cyanobacteria is a wild-wide problem in eutrophic lakes. In some cases, such blooms of cyanobacteria produce microcystins, which are known as the potent hepatotoxin and liver tumor promoter. Microcystins are cyclic heptapeptides and are non-ribosomally synthesized. For the regulation of the production of microcystins by toxic cyanobacteria, we have investigated their biosynthesis. Initially, we carried out the detection, isolation and structural determination of non-toxic peptides produced together with microcystins by toxic cyanobacteria. As shown in Table 1, the isolated non-toxic peptides were classified into several groups on the basis of their structures. The results suggested that the production of these non-toxic peptides is closely related to that of microcystins. In the course of our investigations, Shirai et al. recently identified whole microcystin synthetase genes in the toxic cyanobacterium, Microcystis aeruginosa K-139, which produces 3-desmethyl and 3,7-didesmethyl microcystin-LR (Fig. 1, 2(a)). Furthermore, the synthetic genes of a peptide other than microcystins were also isolated from the same strain (Fig. 2(b)). In this study, we carried out the isolation and structural determination of the corresponding peptide to the synthetic genes isolated. As the result, we isolated a non-toxic peptide designated as micropeptin K139 (1) together with two microcystins from M. aeruginosa K-139. Micropeptin K139 (1) is a cyclic depsipeptide composed of seven amino acids, L-Ile x2, L-MeTyr, L-Ahp, L-Arg, L-Thr, L-Asp, and a fatty acid, HA, and belongs to a group of non-toxic peptides (Fig. 1). The sequence of the constituent segments in 1 and its structure were determined by 2D-NMR techniques, and the advanced Marfey's method was used to determine their absolute configurations. The findings about the structure of micropeptin K139 (1) and the isolated peptide synthetase genes other than those of microcystins indicated that these synthetic genes are for a nontoxic peptide, 1. Therefore, the result suggested that non-toxic peptides are also non-ribosomally synthesized as well as microcystins. The investigation of the genetic relationship between microcystins and the co-producing peptides is in progress., Symposium on the chemistry of natural products
Symposium on the Chemistry of Natural Products, Oct. 2000, [Reviewed]

Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin
Nishizawa; T.; A. Ueda; M. Asayama; K. Fujii; K. Harada; K. Ochi; M. Shirai, Lead, The peptide synthetase gene operon, which consists of mcyA, mcyB, anti mcyC, for the activation and incorporation of the five amino acid constituents of microcystin has been identified [T. Nishizawa et al. (1999) J. Biochem. 126, 52-529]. By sequencing an additional 34 kb of DNA from microcystin-producing Microcystis aeruginosa K-139, we identified the residual microcystin synthetase gene operon, which consists of mcyD, mcyE, mcyF,and mcyG, in the opposite orientation to the mcyABC operon. McyD consisted of two polyketide synthase modules, and McyE contained a polyketide synthase module at the N-terminus and a peptide synthetase module at the C-terminus. McyF was found to exhibit similarity to amino acid racemase, McyG consisted of a peptide synthetase module at the N-terminus and a polyketide synthase at the C-terminus, The microcystin synthetase gene cluster was conserved in another microcystin-producing strain, Microcystis sp, S-70, which produces Microcystin-LR, -RR, and -YR. Insertional mutagenesis of mcyA, mcyD, or mcyE in Microcystis sp, S-70 abolished microcystin production. In conclusion, the mcyDEFG operon is presumed to be responsible for 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) biosynthesis, and the incorporation of Adda and glutamic acid into the microcystin molecule., Oxford University Press
J. Biochem., May 2000, [Reviewed]

Genetic analysis of the peptide synthetase genes for a cyclic heptapeptide microcystin in Microcystis spp.
T Nishizawa; M Asayama; K Fujii; K Harada; M Shirai, Peptide-synthetase-encoding DNA fragments were isolated by a PCR-based approach from the chromosome of Microcystis aeruginosa K-139, which produces cyclic heptapeptides, 7-desmethylmicrocystin-LR and 3,7-didesmethylmicrocystin-LR. Three open reading frames (mcyA, mcyB, mcyC) encoding microcystin synthetases were identified in the gene cluster. Sequence analysis indicated that McyA (315 kDa) consists of two modules with an N-methylation domain attached to the first and an epimerization domain attached to the second; McyB (242 kDa) has two modules, and McyC (147 kDa) contains one module with a putative C-terminal thioesterase domain. Conserved amino acid sequence motifs for ATP binding, ATP hydrolysis, adenylate formation, and 4'-phosphspantetheine attachment were identified by sequence comparison with authentic peptide synthetase. Insertion mutations in mcyA, generated by homologous recombination, abolished the production of both microcystins in ill. aeruginosa K-139, Primer extension analysis demonstrated light-dependent mcy expression. Southern hybridization and partial DNA sequencing analyses of six microcystin-producing and two non-producing Microcystis strains suggested that the microcystin-producing strains contain the mcy gene and the non-producing strains can be divided into two groups, those possessing no mcy genes and those with mcy genes., JAPANESE BIOCHEMICAL SOC
JOURNAL OF BIOCHEMISTRY, Sep. 1999, [Reviewed]

Effects of no-tillage and cover crop application on methane emissions and rice growth in organic rice paddy
Sakoda Midori; Matsubayashi Chisaki; Nakayama Honoka; Asagi Naomi; Nishizawa Tomoyasu; Komatsuzaki Masakazu
Abstracts of Meeting of the CSSJ, 26 Sep. 2024

P6-1-5 カバークロップを利用した有機水田における微生物資材の接種が水稲の生育・収量および窒素吸収に及ぼす影響（6-1 水田土壌肥沃度 2023年度愛媛大会）
加来 嵩時; 高嶋 直哉; 武藤 光輝; 菅井 純; 遠山 佳甫; 迫田 翠; 浅木 直美; 伊沢 剛; 西澤 智康; 小松﨑 将一
日本土壌肥料学会講演要旨集, 04 Sep. 2023

28 道路緑地における植生管理が法面土壌の化学性および物理性に及ぼす影響ついて（関東支部講演会）
宮﨑 永遠; バティン一 希; 鈴木 雄吾; 西澤 智康; 堅田 元喜; 榎本 忠夫; 高瀬 唯; 及川 真平; 坂上 伸生
日本土壌肥料学会講演要旨集, 04 Sep. 2023

24 継続的な不耕起農法によって形成される土壌団粒の物質循環系メタゲノム解析（関東支部講演会）
浦本 匠; 郭 永; 迫田 翠; 坂上 伸生; 小松﨑 将一; 太田 寛行; 西澤 智康
日本土壌肥料学会講演要旨集, 04 Sep. 2023

P3-2-4 Azoarcus 属細菌を接種した陸稲を栽培する土壌の細菌叢解析（3-2 土壌生物の応用と制御 2023年度愛媛大会）
迫田 翠; 浦本 匠; 浅木 直美; 坂上 伸生; 妹尾 啓史; 西澤 智康
日本土壌肥料学会講演要旨集, 04 Sep. 2023

P5-1-1 三宅島2000年噴火後の植生回復過程における土壌の有機・無機成分の変化について（5-1 土壌生成・分類 2023年度愛媛大会）
竹内 琴音; 川崎 愛希; 坂上 伸生; 西澤 智康; 太田 寛行
日本土壌肥料学会講演要旨集, 04 Sep. 2023

29 三宅島2000年噴火後の植生回復過程における土壌生成と有機物の集積（関東支部講演会）
竹内 琴音; 宇賀神 温; 西澤 智康; 太田 寛行; 坂上 伸生
日本土壌肥料学会講演要旨集, 04 Sep. 2023

20 環境保全型農法連用土壌におけるN₂O還元菌の多様性と集積分離培養（関東支部講演会）
郭 永; 阿蘇 日和; 浦本 匠; 迫田 翠; 小松崎 将一; 西澤 智康
日本土壌肥料学会講演要旨集, 04 Sep. 2023

31 駒止湿原開墾跡地における土着微生物を用いたブナ植樹試験 −植樹後5年の生育経過−（関東支部講演会）
轟 丈瑠; 郭 永; 野口 愛; 西澤 智康; 成澤 才彦; 坂上 伸生
日本土壌肥料学会講演要旨集, 04 Sep. 2023

23 耕起から不耕起への転換が土壌理化学性に及ぼす影響（関東支部講演会）
遠山 佳甫; 淺木 直美; 迫田 翠; 浦本 匠; 郭 永; 西澤 智康
日本土壌肥料学会講演要旨集, 04 Sep. 2023

6-2-6 耕起から不耕起への転換が畑地土壌の窒素動態に及ぼす影響（6-2 畑地土壌肥沃度 2023年度愛媛大会）
遠山 佳甫; 淺木 直美; 迫田 翠; 郭 永; 西澤 智康
日本土壌肥料学会講演要旨集, 04 Sep. 2023

Molecular ecological analysis of microbial communities regarding nitrogen-cycle within lake sediment in Lake Kasumigaura (Lake Kitaura)
正田岳志; 大竹礼二; 早川美久; GUO Yong; 迫田翠; 迫田翠; 中里亮治; 西澤智康; 西澤智康; 西澤智康; 西澤智康
日本微生物生態学会大会(Web), 2023

耕起から不耕起への転換が土壌理化学性に及ぼす影響
遠山佳甫; 淺木直美; 迫田翠; 浦本匠; 郭永; 西澤智康; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2023

三宅島2000年噴火後の植生回復過程における土壌生成と有機物の集積
竹内琴音; 宇賀神温; 西澤智康; 太田寛行; 坂上伸生
日本土壌肥料学会講演要旨集(Web), 2023

駒止湿原開墾跡地における土着微生物を用いたブナ植樹試験-植樹後5年の生育経過-
轟丈瑠; 郭永; 野口愛; 西澤智康; 成澤才彦; 坂上伸生
日本土壌肥料学会講演要旨集(Web), 2023

道路緑地における植生管理が法面土壌の化学性および物理性に及ぼす影響ついて
宮崎永遠; バティン一希; 鈴木雄吾; 西澤智康; 堅田元喜; 榎本忠夫; 高瀬唯; 及川真平; 坂上伸生
日本土壌肥料学会講演要旨集(Web), 2023

環境保全型農法連用土壌におけるN₂O還元菌の多様性と集積分離培養
郭永; 阿蘇日和; 浦本匠; 迫田翠; 小松崎将一; 小松崎将一; 西澤智康; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2023

継続的な不耕起農法によって形成される土壌団粒の物質循環系メタゲノム解析
浦本匠; 郭永; 迫田翠; 坂上伸生; 坂上伸生; 小松崎将一; 小松崎将一; 太田寛行; 西澤智康; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2023

三宅島2000年噴火後の植生回復過程における土壌の有機・無機成分の変化について
竹内琴音; 川崎愛希; 坂上伸生; 坂上伸生; 西澤智康; 西澤智康; 太田寛行
日本土壌肥料学会講演要旨集(Web), 2023

霞ケ浦(北浦)湖底堆積物における薬剤耐性細菌の推定
早川美久; 郭永; 迫田翠; 迫田翠; 正田岳志; 中里亮治; 西澤智康; 西澤智康; 西澤智康
日本土壌微生物学会大会講演要旨集(CD-ROM), 2023

カバークロップを利用した有機水田における微生物資材の接種が水稲の生育・収量および窒素吸収に及ぼす影響
加来嵩時; 高嶋直哉; 武藤光輝; 菅井純; 遠山佳甫; 迫田翠; 浅木直美; 伊沢剛; 西澤智康; 小松崎将一
日本土壌肥料学会講演要旨集(Web), 2023

耕起から不耕起への転換が畑地土壌の窒素動態に及ぼす影響
遠山佳甫; 淺木直美; 淺木直美; 迫田翠; 迫田翠; 郭永; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2023

Identification and distribution of fruiting body-forming ectomycorrhizal fungi on soil surface of the Burial graves for children, the Sannai Maruyama Site
長尾理伶; 轟丈瑠; 山田紀代美; 佐々木淑美; 石崎武志; 佐藤嘉則; 西澤智康
日本文化財科学会大会研究発表要旨集, 2023

Azoarcus属細菌を接種した陸稲を栽培する土壌の細菌叢解析
迫田翠; 迫田翠; 浦本匠; 浅木直美; 浅木直美; 浅木直美; 坂上伸生; 坂上伸生; 坂上伸生; 妹尾啓史; 妹尾啓史; 西澤智康; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2023

1 不耕起畑地に形成される土壌団粒が土壌窒素循環に及ぼす影響（関東支部講演会 2021年度支部講演会）
浦本 匠; 坂上 伸生; 迫田 翠; 郭 永; 小松﨑 将一; 太田 寛行; 西澤 智康
日本土壌肥料学会講演要旨集, 05 Sep. 2022

P3-1-10 メタン排出量の異なるイネ品種の根圏土壌セルラーゼ活性の比較（3-1 土壌生物の生態と機能 2022年度東京大会）
酒井 順子; 迫田 翠; 西澤 智康; 常田 岳志
日本土壌肥料学会講演要旨集, 05 Sep. 2022

Environmental characteristics and application of symbiotic fungi for planting of beech seedlings in the abandoned cropland around Komado-Shitsugen
轟丈瑠; GUO Yong; 野口愛; 高島勇介; 西澤智康; 成澤才彦; 坂上伸生
日本生態学会大会講演要旨(Web), 2022

Free fatty acid production in engineered Escherichia coli with enhancement of malonyl-CoA biosynthesis
濱田美志; 中平洋一; 西澤智康; 長南茂
日本生物工学会大会講演要旨集, 2022

不耕起畑地に形成される土壌団粒が土壌窒素循環に及ぼす影響
浦本匠; 坂上伸生; 坂上伸生; 迫田翠; 郭永; 小松崎将一; 小松崎将一; 太田寛行; 西澤智康; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2022

メタン排出量の異なるイネ品種の根圏土壌セルラーゼ活性の比較
酒井順子; 迫田翠; 西澤智康; 常田岳志
日本土壌肥料学会講演要旨集(Web), 2022

Exporing the Potential of Poland Indigenous Phyllobacterium in Soybean Cultivation under Cold Conditions
渡邊陸; RAMIREZ Maria Daniela Artigas; RAMIREZ Maria Daniela Artigas; LEWANDOWSKA Sylwia; ベリングラード(木村)園子ドロテア; GUO Yong; 西澤智康; 梶田忠; 安田美智子; 安掛真一郎; 大津直子
植物微生物研究会研究交流会講演要旨集, 2022

三宅島火山灰堆積物において窒素循環を駆動する微生物群集のメタゲノミクス
宇賀神温; 郭永; 坂上伸生; 上條隆志; 太田寛行; 西澤智康
日本土壌微生物学会大会講演要旨集(CD-ROM), 2022

P8-1-13 道路緑地におけるクズの繁茂が土壌の無機元素動態に及ぼす影響（8-1 環境保全 2021年度北海道大会）
宮﨑 永遠; 竹内 琴音; 西澤 智康; 堅田 元喜; 福島 慶太郎; 榎本 忠夫; 高瀬 唯; 及川 真平; 坂上 伸生
日本土壌肥料学会講演要旨集, 03 Sep. 2021

P8-1-12 駒止湿原開墾跡地における植生および土壌環境の多様性とブナ実生の生育について（8-1 環境保全 2021年度北海道大会）
坂上 伸生; 轟 丈瑠; 郭 永; 西澤 智康; 村田 智吉; 渡邊 眞紀子
日本土壌肥料学会講演要旨集, 03 Sep. 2021

13 微生物を接種した水稲栽培によるメタン排出低減と玄米の外観品質の向上（関東支部講演会 2020年度支部講演会）
迫田 翠; 常田 岳志; 酒井 順子; 妹尾 啓史; 西澤 智康
日本土壌肥料学会講演要旨集, 03 Sep. 2021

6-2-5 Effect of conservation agriculture on the amount and quality of fractionated organic matter of Andosols in a long-term experiment in Ibaraki, Japan（6-2 畑地土壌肥沃度 2021年度北海道大会）
杉原 創; 小松崎 将一; 西澤 智康; 田中 治夫
日本土壌肥料学会講演要旨集, 03 Sep. 2021

Change of structure and dynamics of microbe community in the 2000 year volcanic ash
太田寛行; 西澤智康
日本生態学会大会講演要旨(Web), 2021

好酸性鉄酸化細菌のTCA回路改変によるエチレン生成
加藤達弘; 中村孝道; 谷口惠梨; 沖田貴弘; 久留主泰朗; 西澤智康
日本ゲノム微生物学会年会要旨集, 2021

Effect of conservation agriculture on the amount and quality of fractionated organic matter of Andosols in a long-term experiment in Ibaraki, Japan
ADUHENE-CHINBUAH Jeannette; 杉原創; 小松崎将一; 西澤智康; 田中治夫
日本土壌肥料学会講演要旨集(Web), 2021

微生物を接種した水稲栽培によるメタン排出低減と玄米の外観品質の向上
迫田翠; 常田岳志; 常田岳志; 酒井順子; 妹尾啓史; 妹尾啓史; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2021

駒止湿原開墾跡地における植生および土壌環境の多様性とブナ実生の生育について
坂上伸生; 轟丈瑠; 郭永; 西澤智康; 村田智吉; 渡邊眞紀子
日本土壌肥料学会講演要旨集(Web), 2021

道路緑地におけるクズの繁茂が土壌の無機元素動態に及ぼす影響
宮崎永遠; 竹内琴音; 西澤智康; 堅田元喜; 福島慶太郎; 榎本忠夫; 高瀬唯; 及川真平; 坂上伸生
日本土壌肥料学会講演要旨集(Web), 2021

三宅島2000年火山灰堆積物における微生物生態系解析:20年間の遷移
宇賀神温; 坂上伸生; 上條隆志; 太田寛行; 西澤智康
日本土壌微生物学会大会講演要旨集(CD-ROM), 2021

駒止湿原開墾跡地でのブナ林再生に向けた植樹試験における共生菌類の利用
轟丈瑠; 郭永; 西澤智康; 高島勇介; 渡邊眞紀子; 成澤才彦; 坂上伸生
日本土壌微生物学会大会講演要旨集(CD-ROM), 2021

8-1-33 高速道路法面における除草剤の散布が土壌の化学性および土壌微生物群集に与える影響（8-1 環境保全 2020年度岡山大会）
坂上 伸生; 迫田 翠; 竹内 琴音; 西澤 智康; 堅田 元喜; 福島 慶太郎; 榎本 忠夫; 高瀬 唯; 及川 真平
日本土壌肥料学会講演要旨集, 01 Sep. 2020

3-1-10 CLPP解析に基づく環境保全型畑地土壌の炭素利用性（3-1 土壌生物の生態と機能 2020年度岡山大会）
阿蘇 日和; 荒木 肇; 小松崎 将一; 太田 寛行; 西澤 智康
日本土壌肥料学会講演要旨集, 01 Sep. 2020

29 イネ種子への微生物接種は水田からのメタン排出に影響を及ぼす（関東支部講演会，2019年度各支部会）
迫田 翠; 西脇 淳子; 常田 岳志; 妹尾 啓史; 西澤 智康
日本土壌肥料学会講演要旨集, 01 Sep. 2020

3-1-13 イネ種子への微生物接種によりメタン排出が低減した水田土壌の微生物性解析（3-1 土壌生物の生態と機能 2020年度岡山大会）
迫田 翠; 常田 岳志; 酒井 順子; 妹尾 啓史; 西澤 智康
日本土壌肥料学会講演要旨集, 01 Sep. 2020

4 土壌環境ストレスがフザリウム属菌nit 変異株の生残に与える影響（関東支部講演会，2019年度各支部会）
星 光輝; 唐澤 敏彦; 大友 量; 橋本 知義; 西澤 智康
日本土壌肥料学会講演要旨集, 01 Sep. 2020

風連鍾乳洞内に生育する照明植生の微生物叢解析
黒坂愛美; 佐藤嘉則; 片山葉子; 朽津信明; 西澤智康
日本ゲノム微生物学会年会要旨集, 2020

イネ種子への微生物接種によりメタン排出が低減した水田土壌の微生物性解析
迫田翠; 常田岳志; 常田岳志; 酒井順子; 妹尾啓史; 妹尾啓史; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2020

CLPP解析に基づく環境保全型畑地土壌の炭素利用性
阿蘇日和; 荒木肇; 小松崎将一; 太田寛行; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2020

土壌環境ストレスがフザリウム属菌nit変異株の生残に与える影響
星光輝; 唐澤敏彦; 大友量; 橋本知義; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2020

高速道路法面における除草剤の散布が土壌の化学性および土壌微生物群集に与える影響
坂上伸生; 迫田翠; 竹内琴音; 西澤智康; 堅田元喜; 福島慶太郎; 榎本忠夫; 高瀬唯; 及川真平
日本土壌肥料学会講演要旨集(Web), 2020

イネ種子への微生物接種は水田からのメタン排出に影響を及ぼす
迫田翠; 西脇淳子; 常田岳志; 妹尾啓史; 妹尾啓史; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2020

人為的攪乱により形成された鍾乳洞内照明植生の微生物生態学的解析
黒坂愛美; 佐藤嘉則; 片山葉子; 朽津信明; 西澤智康
日本土壌微生物学会大会講演要旨集(CD-ROM), 2020

駒止湿原開墾跡地におけるブナ共生菌類を利用した植樹試験の試行
轟丈瑠; 郭永; 高島勇介; 渡邊眞紀子; 西澤智康; 成澤才彦; 坂上伸生
日本土壌微生物学会大会講演要旨集(CD-ROM), 2020

高速道路法面における除草剤の散布が土壌微生物群集に与える影響
迫田翠; 坂上伸生; 郭永; 西澤智康; 堅田元喜; 榎本忠夫; 高瀬唯; 及川真平
日本土壌微生物学会大会講演要旨集(CD-ROM), 2020

石人山古墳石棺表面から分離した藻類のメタゲノム解析
小沼奈那美; 加藤徳子; 郭永; 佐藤嘉則; 朽津信明; 西澤智康; 西澤智康
日本ゲノム微生物学会年会要旨集, 2020

P3-1-2 カバークロップ施用畑地土壌における土壌団粒微生物の動態解析（3-1 土壌生物の生態と機能，2019年静岡大会）
阿蘇 日和; 中根 麻冴美; 荒木 肇; 小松崎 将一; 太田 寛行; 西澤 智康
日本土壌肥料学会講演要旨集, 03 Sep. 2019

P3-2-2 イネ（日本晴）初期生育を促進するAzoarcus属細菌の接種効果：イネの質的向上と根域土壌微生物群集への影響（3-2 土壌生物の応用と制御，2019年静岡大会）
迫田 翠; 佐々木 拓朗; 増冨 祐司; 坂上 伸生; 妹尾 啓史; 西澤 智康
日本土壌肥料学会講演要旨集, 03 Sep. 2019

平成29年度無人飛行機を用いた放射性プルーム測定技術の確立(受託研究)—Development of Measurement System of Radioactive Plume using Unmanned Airplane in the Fiscal Year 2017 (Contract Research)
眞田 幸尚; 西澤 幸康; 越智 康太郎; 結城 洋一; 石﨑 梓; 長田 直之
JAEA-research, Jan. 2019

Ⅵ-4 畑地土壌団粒における微生物群集の構造・動態変化 農地土壌管理が微生物性に与えるインパクト（Ⅵ 土壌団粒構造と土壌プロセス2，2019年静岡大会）
西澤 智康; 太田 寬行
日本土壌肥料学会講演要旨集, 2019

ブタノール生成Clostridium属細菌の比較ゲノム解析
黒坂愛美; 郭永; 上原研人; 長南茂; 久留主泰朗; 太田寛行; 西澤智康
日本ゲノム微生物学会年会要旨集, 2019

ノニルフェノール分解性細菌Sphingobium amiense DSM 16289^TおよびSphingobium cloacae JCM 10874^Tの全ゲノム解析による代謝全体像の比較
大塚美奈; 郭永; 西澤智康; 西澤智康; 久留主泰朗; 久留主泰朗; 太田寛行; 太田寛行
日本ゲノム微生物学会年会要旨集, 2019

窒素固定遺伝子を有する脱窒細菌によるN₂O固定の検証
鈴木里俊; 早川智恵; 早川智恵; 増田曜子; 西澤智康; 菅原雅之; 嵐田遥; 南澤究; 磯部一夫; 妹尾啓史; 妹尾啓史
日本微生物生態学会大会(Web), 2019

Mortierella humilis/verticillata complexとその内生細菌のトマト生育への影響について
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本微生物生態学会大会(Web), 2019

根部エンドファイトVeronaeopsis simplex Y34のトマト根部微生物叢への時空間的な影響
野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本微生物生態学会大会(Web), 2019

カバークロップ施用畑地土壌における土壌団粒微生物の動態解析
阿蘇日和; 中根麻冴美; 荒木肇; 小松崎将一; 太田寛行; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2019

窒素固定遺伝子を有する脱窒細菌によるN₂O固定の検証
鈴木里俊; 早川智恵; 増田曜子; 西澤智康; 菅原雅之; 嵐田遥; 南澤究; 磯部一夫; 妹尾啓史; 妹尾啓史
日本土壌肥料学会講演要旨集(Web), 2019

イネ(日本晴)初期生育を促進するAzoarcus属細菌の接種効果:イネの質的向上と根域土壌微生物群集への影響
迫田翠; 佐々木拓朗; 増冨祐司; 増冨祐司; 坂上伸生; 坂上伸生; 妹尾啓史; 妹尾啓史; 西澤智康; 西澤智康
日本土壌肥料学会講演要旨集(Web), 2019

畑地土壌団粒における微生物群集の構造・動態変化 農地土壌管理が微生物性に与えるインパクト
西澤智康; 太田寛行
日本土壌肥料学会講演要旨集(Web), 2019

イネ科カバークロップ-不耕起栽培畑地土壌の耐水性団粒構造と微生物叢の解析
阿蘇日和; 中根麻冴美; 荒木肇; 小松崎将一; 太田寛行; 西澤智康
日本土壌微生物学会講演要旨集, 2019

根部エンドファイトVeronaeopsis simplex Y34の内外生細菌Rhizobium pusense Y9およびその近縁種が宿主菌類に及ぼす影響
橋本実佳; 郭永; 西澤智康; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

環境ストレスを与えた圃場におけるフザリウム属菌nit変異株の生残性の解析
星光輝; 橋本知義; 西澤智康
日本土壌微生物学会講演要旨集, 2019

テトラサイクリン耐性乳酸菌と薬剤を添加した土壌ミクロコズムにおける微生物群集の動態解析
矢用もも; 西澤智康
日本土壌微生物学会講演要旨集, 2019

高温および有機態窒素条件におけるMortierella humilisとその内生細菌がトマト生育に及ぼす影響
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

Isolation and characterization of endophytic microbes associated with the pioneer grass colonizing on the recent volcanic deposits in the island of Miyake, Japan
孫寒; 西澤智康; 成澤才彦; 太田寛行
日本土壌微生物学会講演要旨集, 2019

窒素固定能を有する脱窒細菌によるN₂O固定
鈴木里俊; 早川智恵; 増田曜子; 西澤智康; 菅原雅之; 嵐田遥; 南澤究; 磯部一夫; 妹尾啓史; 妹尾啓史
日本土壌微生物学会講演要旨集, 2019

Veronaeopsis simplex Y34接種トマトの根圏微生物叢の経時的モニタリング
野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2019

もう1つの温室効果ガス発生源-作物残さに由来する一酸化二窒素の発生とその原因微生物の解析
多胡香奈子; 徳田進一; 郭永; 黒岩恵; 中村知喬; 西澤智康; 諏訪裕一; 早津雅仁
日本土壌微生物学会講演要旨集, 2019

淡水由来微生物B-9株の機能解明に関する研究(10)-Microcystin分解性細菌B-9株の全ゲノムの解析-
金海燕; 西澤智康; 郭勇; 西澤明人; 朴東虎; 加藤創; 辻清美; 原田健一; 原田健一
日本薬学会年会要旨集(CD-ROM), 2019

Mortierella属糸状菌に内生するMycoavidus属細菌のゲノム縮小化
郭永; 高島勇介; SHARMIN Dilruba; 佐藤嘉則; 成澤才彦; 太田寛行; 西澤智康
日本土壌微生物学会講演要旨集, 2019

Azoarcus sp.KH32C株が保有するポリケチド合成酵素遺伝子の分子遺伝学的解析
西澤智康; 迫田翠; 花岡彩乃; 鈴木義人; 太田寛行; 山下竜司; 兼井啓介; 原田健一; 妹尾啓史
日本ゲノム微生物学会年会要旨集, 2018

初成土壌形成と微生物:炭素集積と微生物群集の遷移に関する考察
太田寛行; 西澤智康; 郭永; 平野明則; 上條隆志
日本土壌肥料学会講演要旨集, 2018

三宅島2000年噴火後の土壌層位形成-各層における化学性状の遷移-
海老原諒子; 平野明則; 坂上伸生; 太田寛行; 西澤智康
日本土壌肥料学会講演要旨集, 2018

根部エンドファイトVeronaeopsis simplex Y34から分離されたRhizobium pusense Y9株とその近縁種が宿主に及ぼす影響
橋本実佳; 郭永; 西澤智康; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

土壌環境ストレスがフザリウム属菌nit変異株の生残性に及ぼす影響
星光輝; 西澤智康; 橋本知義
日本土壌微生物学会講演要旨集, 2018

Veronaeopsis simplex Y34資材を用いたハウス栽培におけるトマト根部微生物叢への影響
野口愛; 高田圭太; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

土壌団粒と微生物群集の動態変化:農地土壌管理のインパクトを考える
中根麻冴美; 雫田麻衣; 小松崎将一; 橋本知義; 西澤智康; 太田寛行
日本土壌微生物学会講演要旨集, 2018

有機質資材を投入した圃場の土壌細菌群集解析とテトラサイクリン耐性細菌の同定
矢用もも; 西澤智康
日本土壌微生物学会講演要旨集, 2018

糸状菌内生細菌Mycoavidusの二次代謝産物生合成遺伝子の系統解析
郭永; SHARMIN Dilruba; 高島勇介; 成澤才彦; 太田寛行; 西澤智康
日本土壌微生物学会講演要旨集, 2018

Mortierella属菌とその内生細菌がトマト生育に及ぼす影響
松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
日本土壌微生物学会講演要旨集, 2018

Microcystin分解性細菌B-9株の全ゲノムの解析
金海燕; 西澤智康; 郭勇; 西澤明人; 朴虎東; 加藤創; 辻清美; 原田健一
日本陸水学会大会講演要旨集, 2018

陸域環境における脱窒微生物群集組成の比較
畔上洋一; 藤村玲子; 磯部一夫; 魏魏; 角田洋子; 白鳥豊; 西澤智康; 木庭啓介; 大手信人; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 05 Sep. 2017

三宅島初成土壌に生育するパイオニア植物根圏のニトロゲナーゼ活性と根圏のBurkholderiaceae科細菌
海老原諒子; 郭永; 西澤智康; 上條隆志; 太田寛行
日本土壌肥料学会講演要旨集, 2017

三宅島火山灰堆積物に新たに形成された土壌層位のメタゲノム解析
平野明則; 郭永; 坂上伸生; 西澤智康; 上條隆志; 太田寛行
日本土壌肥料学会講演要旨集, 2017

イネ生育促進効果をもつ脱窒細菌がイネ根域土壌細菌群集に及ぼす影響
迫田翠; 水澤舞花; 郭永; 塩津文隆; 坂上伸生; 増冨祐司; 太田寛行; 藤原徹; 妹尾啓史; 西澤智康
日本土壌肥料学会講演要旨集, 2017

不耕起と耕起栽培畑地の耐水性土壌団粒における微生物動態
西澤智康; 中根麻冴美; 雫田麻衣; 橋本知義; 小松崎将一; 太田寛行
日本土壌肥料学会講演要旨集, 2017

不耕起・草生栽培畑地土壌の微生物生態学:耐水性団粒構造と微生物の分布について
太田寛行; 雫田麻衣; 中根麻冴美; 西澤智康; 橋本知義; 西脇淳子; 小松崎将一
日本土壌肥料学会講演要旨集, 2017

平成27年9月関東・東北豪雨の影響を受けた農作物の無機元素組成に関する調査報告
坂上伸生; 齋藤明葉; 西澤智康; 成澤才彦; 伊藤哲司
日本地理学会発表要旨集, 2017

クリンカー添加が茶殻堆肥化における微生物群集構造と基質資化能の多様性に与える影響
森田展樹; 上野秀人; 当真要; 岡寛; 西澤智康; 雫田麻衣
日本土壌肥料学会講演要旨集, 2017

太陽熱消毒した畑地土壌の微生物群集構造の解析
星光輝; 橋本知義; 西澤智康; 太田寛行
日本微生物生態学会大会(Web), 2017

土壌層位形成過程や土壌団粒構造の微生物群集構造解析
西澤智康; 太田寛行
日本微生物生態学会大会(Web), 2017

外来パントテン酸キナーゼ遺伝子の大腸菌ゲノムへの導入によるコエンザイムA生合成経路の強化
根岸剛志; 西澤智康; 三浦隆匡; 長南茂
日本微生物生態学会大会(Web), 2017

Soil survey on vegetation recovery of the abandoned cropland around Komado Shitsugen Moor, Fukushima Prefecture
SAKAGAMI Nobuo; INOSE Yuumi; GUO Yong; NISHIZAWA Tomoyasu; NARISAWA Kazuhiko; WATANABE Makiko
Proceedings of the General Meeting of the Association of Japanese Geographers, 2017

土壌微生物が創る共生の世界 －その先端的研究事例と農業への応用的研究展開4．糸状菌に共生する細菌その機能と生態－
太田寛行; 西澤智康; 松岡勇人; 高島勇介; 成澤才彦
日本土壌肥料学雑誌, Aug. 2016, [Reviewed], [Invited]

石人山古墳装飾石棺表面に形成した着生生物群集の構造解析—Molecular Analysis of the Epiphytic Microbial Community Formed on Surfaces of Decorative Stone Sarcophagus at Sekijinsan Tumulus
佐藤 嘉則; 西澤 智康; 小沼 奈那美; 犬塚 将英; 森井 順之; 木川 りか; 朽津 信明
保存科学, 2016

日本微生物生態学会第30回大会・第7回JTKシンポジウムの報告(大会開催報告)
太田 寛行; 西澤 智康; 長南 茂
日本微生物生態学会誌, 2016

自然農法圃場に形成される土壌団粒の組成割合および微生物群集構造解析
雫田麻衣; 中根麻冴美; 橋本知義; 小松崎将一; 西脇淳子; 太田寛行; 西澤智康
日本土壌肥料学会講演要旨集, 2016

クリンカー添加割合が茶殻堆肥の化学性および生物的特性に与える影響
森田展樹; 上野秀人; 当真要; 岡寛; 西澤智康; 雫田麻衣
日本土壌肥料学会講演要旨集, 2016

ブタノール生産性Clostridium属細菌のゲノム情報に基づく発酵代謝系の分子遺伝学的解析
上原研人; 秋山真成美; 金本美穂; 西澤智康; 長南茂; 新田洋司; 久留主泰朗; 太田寛行
日本微生物生態学会大会(Web), 2016

低アンモニア条件下での土壌還流法におけるアンモニア酸化菌の動態解析
金本美穂; 西澤智康; 太田寛行
日本微生物生態学会大会(Web), 2016

不耕起栽培畑地土壌における耐水性団粒の微生物群集メタゲノム解析
中根麻冴美; 雫田麻衣; 西澤智康; 小松崎将一; 橋本知義; 太田寛行
日本微生物生態学会大会(Web), 2016

不耕起栽培畑地土壌における耐水性団粒内の微生物群集構造解析
中根麻冴美; 雫下麻衣; 西澤智康; 小松崎将一; 橋本知義; 太田寛行
日本土壌微生物学会講演要旨集, 2016

三宅島初成土壌に生育するパイオニア植物根域のニトロゲナーゼ活性と根圏細菌の解析
海老原諒子; 平野明則; 郭永; 西澤智康; 上條隆志; 太田寛行
日本微生物生態学会大会(Web), 2016

3-1-15 三宅島火山灰堆積物で形成された土壌層位における微生物群集構造解析（3-1 土壌生物の生態と機能，2016年度佐賀大会）
平野 明則; 郭 永; 西澤 智康; 上條 隆志; 成澤 才彦; 太田 寛行
日本土壌肥料学会講演要旨集, 2016

Mortierella属糸状菌に内生する細菌の多様性解析
生田目光; 高島勇介; 佐藤嘉則; 西澤智康; 成澤才彦; 太田寛行
日本土壌微生物学会講演要旨集, 2016

糸状菌に内生する細菌の系統と相互作用に関する考察
太田寛行; 佐藤嘉則; 西澤智康; 成澤才彦
日本土壌微生物学会講演要旨集, 2016

3-1-3 脱窒細菌Azoarcus属KH32C株接種による窒素無施肥水田土壌でのイネ生育促進（3-1 土壌生物の生態と機能，2016年度佐賀大会）
西澤 智康; 水澤 舞花; 塩津 文隆; 作田 庄平; 藤原 徹; 太田 寬行; 妹尾 啓史
日本土壌肥料学会講演要旨集, 2016

土壌糸状菌Mortierella elongataに内生する細菌のゲノム特性の解析(2015年度大会講演要旨)
大島 翔子; 藤村 玲子; 水上 沙紀; 佐藤 嘉則; 西澤 智康; 成澤 才彦; 太田 寛行
土と微生物, 01 Oct. 2015

土壌糸状菌に内生するBurkholderiaceae科細菌の比較ゲノム解析
西澤智康; 藤村玲子; 大島翔子; 大島健志朗; 郭永; 佐藤嘉則; 服部正平; 服部正平; 成澤才彦; 太田寛行
日本土壌肥料学会講演要旨集, 09 Sep. 2015

農耕地からのN_2Oガス発生を削減し作物生産性を向上する脱窒菌株の探索 : N_2O削減微生物肥料の開発を目指して(2015年度大会講演要旨)
白鳥 豊; 角田 洋子; 西澤 智康; 高谷 範子; 永峰 賢; 磯部 一夫; 大塚 重人; 妹尾 啓史
土と微生物, 2015

刈敷を活用した不耕起・草生畑地の土壌微生物群集構造解析
雫田麻衣; 小松崎将一; 成澤才彦; 太田寛行; 西澤智康
土と微生物, 2015

土壌生成プロセスにおける微生物の役割
太田寛行; 西澤智康
日本土壌微生物学会講演要旨集, 2015

不耕起・草生畑地土壌中に形成される土壌団粒の真核微生物群集構造解析
雫田麻衣; 中根麻冴美; 西澤智康; 西澤智康; 小松崎将一; 小松崎将一; 成澤才彦; 成澤才彦; 橋本知義; 太田寛行; 太田寛行
日本微生物生態学会大会(Web), 2015

水田土壌における硝酸のアンモニウムへの異化的還元
金子由佳; 磯部一夫; 野尻陽介; 白鳥豊; 西澤智康
日本微生物生態学会大会(Web), 2015

根部エンドファイトVeronaeopsis simplexから分離されたRhizobium sp.Y9株の全ゲノム解析
松岡勇人; KHASTINI Rida; 郭永; 西澤智康; 成澤才彦
日本微生物生態学会大会(Web), 2015

Limnothrix sp.ABRG5-3株のゲノム解読と他種シアノバクテリアゲノムとの比較
田島直幸; 兼崎友; 佐藤修正; 吉川博文; 丸山史人; 黒川顕; 太田啓之; 田畑哲之; 高根澤陽; 西澤智康; 朝山宗彦; 佐藤直樹
日本植物生理学会年会要旨集, 2015

新奇Microcystis属シアノバクテリアの特徴付け
鎭西真理子; 本堂彩花; 菅原将太; 西澤智康; 白井誠; 朝山宗彦
日本農芸化学会関東支部講演要旨集, 2015

三宅島2000年噴火堆積物中の窒素固定細菌に関する研究
平野明則; 郭永; 藤村玲子; 佐藤嘉則; 西澤智康; 上條隆志; 成澤才彦; 太田寛行
土と微生物, 2015

陸域環境における亜硝酸還元酵素遺伝子の分布と組成
畔上洋一; 磯部一夫; 魏魏; 角田洋子; 白鳥豊; 西澤智康; 木庭啓介; 大手信人; 大塚重人; 妹尾啓史
日本微生物生態学会大会(Web), 2015

霞ヶ浦流域畑地および林地土壌表層10mレイヤーの薬剤耐性菌の分布(土壌生態系(soil ecosystem),一般講演,環境微生物系学会合同大会2014講演要旨)
平野 明則; 上原 研人; 金田一 美有; 郭 永; 西澤 智康; 黒田 久雄; 太田 寛行
土と微生物, 01 Oct. 2014

水田におけるバルクおよび根圏土壌細菌群集の構造とその周年変動(土壌生態系(soil ecosystem),一般講演,環境微生物系学会合同大会2014講演要旨)
角田 洋子; 白鳥 豊; 伊藤 英臣; 堀 知行; 西澤 智康; 磯部 一夫; 大塚 重人; 妹尾 啓史
土と微生物, 2014

液状きゅう肥長期連用畑地土壌の窒素循環と微生物生態系(メタゲノムから見える環境と(微)生物の生き様-ここまでわかるメタゲノム解析,シンポジウム(1),環境微生物系学会合同大会2014講演要旨)
石川 美友紀; 奈良岡 雅大; 藤村 玲子; 高見 英人; 新美 洋; 西澤 智康; 太田 寛行
土と微生物, 2014

3-1-8 Dominance of fungal denitrification in cropland soil
魏 巍; 磯部 一夫; 西澤 智康; 白鳥 豊; 大手 信人; 大塚 重人; 妹尾 啓史
Abstracts of the Annual Meetings, Japanese Society of Soil Science and Plant Nutrition, 2014

水田土壌から分離した脱窒細菌による植物の初期生育促進効果(2013年度大会一般講演要旨)
角田 洋子; 西澤 智康; 高谷 範子; 磯部 一夫; 大塚 重人; 妹尾 啓史
土と微生物, 2014

3-1-16 Molecular Analysis of Microbial Communities Associated with Roots of Pioneer Grass Miscanthus condensatus on Recent Miyake-jima Volcanic Deposits
郭 永; 藤村 玲子; 佐藤 嘉則; 西澤 智康; 上條 隆志; 太田 寛行
Abstracts of the Annual Meetings, Japanese Society of Soil Science and Plant Nutrition, 2014

土壌中でのカビ脱窒と生態系の機能
石川美友紀; 奈良岡雅大; 藤村玲子; 高見英人; 新美洋; 西澤智康; 太田寛行
日本土壌微生物学会講演要旨集, 2014

水田におけるバルクおよび根圏土壌細菌群集の構造とその周年変動
角田洋子; 白鳥豊; 伊藤英臣; 堀知行; 西澤智康; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌微生物学会講演要旨集, 2014

糸状菌Mortierella elongataに内生する新属新種細菌の性状解析
大島翔子; 佐藤嘉則; 藤村玲子; 西村歩; 西澤智康; 成澤才彦; 太田寛行
日本土壌微生物学会講演要旨集, 2014

水稲根圏微生物の群集組成とその栽培期間における変動-16S rDNAメタアンプリコンシーケンスによる解析-
角田洋子; 白鳥豊; 伊藤英臣; 堀知行; 西澤智康; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 2014

Identification of N₂O-generating microorganisms in agricultural field by utilizing genomic information
妹尾啓史; 磯部一夫; 巍魏; 白鳥豊; 西澤智康; 西澤智康; 大塚重人; 吉田尚弘; 豊田栄
Institute for Fermentation, Osaka. Research Communications, 2014

Clostridium difficile感染症の発生の危険因子に関する多施設共同ケースコントロール研究
高橋正彦; 高取祐作; 佐藤道子; 作野隆; 成瀬智康; 高林馨; 加藤元彦; 菊池美穂; 西澤俊宏; 藤山洋一; 浦岡俊夫
日本消化器病学会大会(Web), 2014

Limnothrix sp. ABRG5-3株のゲノム配列決定とシアノバクテリアの中での系統関係
田島直幸; 兼崎友; 佐藤修正; 吉川博文; 丸山史人; 黒川顕; 太田啓之; 田畑哲之; 高根澤陽; 西澤智康; 朝山宗彦; 佐藤直樹
日本植物生理学会年会要旨集, 2014

P22-18 霞ヶ浦流域畑地および林地土壌表層10mレイヤーの薬剤耐性菌の分布(ポスター発表)
平野 明則; 上原 研人; 金田一 美有; 郭 永; 西澤 智康; 黒田 久雄; 太田 寛行
日本微生物生態学会講演要旨集, 2014

水田土壌脱窒細菌Azoarcus属KH32C株はイネだけでなくアカクローバーの根にも定着する
角田洋子; 西澤智康; 箱山雅生; 浦口晋平; 藤原徹; 大島健志朗; 服部正平; 磯部一夫; 大塚重人; 妹尾啓史
日本微生物生態学会講演要旨集, 17 Nov. 2013

水田土壌から分離した鍵脱窒菌群のドラフトゲノム解析
浜秀輔; 西澤智康; 上井祐介; 甲斐文彬; 大島健志朗; 服部正平; 磯部一夫; 大塚重人; 妹尾啓史
日本微生物生態学会講演要旨集, 17 Nov. 2013

Plant growth promoting ability of Azoarcus sp. KH32C, a potent N₂O-mitigating denitrifier　　　　　　　　　　　　　　　
Nishizawa; T.; A. Kai; H. Kakuta; K. Isobe; S. Otsuka; T. Hakoyama; S. Uraguchi; T. Fujiwara; K. Senoo
18th International Congress on Nitrogen Fixation, Oct. 2013
Lead

水田土壌から分離した脱窒細菌による植物の初期生育促進効果
角田洋子; 西澤智康; 高谷範子; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌微生物学会講演要旨集, 2013

Verrucomicrobia門に属する土壌細菌Roseimicrobium gellanilyticum DC2a-G7^T株のドラフトゲノム解析
末永卓; 大塚重人; 伊藤英臣; 西澤智康; 磯部一夫; 妹尾啓史
日本ゲノム微生物学会年会要旨集, 2013

土壌から分離されたVerrucomicrobia門細菌の生理性状および潜在的代謝特性
末永卓; 大塚重人; 伊藤英臣; 西澤智康; 磯部一夫; 妹尾啓史
日本微生物生態学会講演要旨集, 2013

畑土壌表面に施用した有機質肥料からの糸状菌脱窒によるN₂O発生
WEI Wei; 磯部一夫; 白鳥豊; 西澤智康; 大塚重人; 妹尾啓史
日本農芸化学会大会講演要旨集(Web), 2013

新奇シアノバクテリアの自己溶菌とポリリン酸蓄積
北崎千富美; 北崎千富美; 沼野節子; 沼野節子; 高根澤陽; 西澤智康; 白井誠; 朝山宗彦; 朝山宗彦
日本農芸化学会関東支部講演要旨集, 2013

OK-005 脱窒細菌Azoarcus属KH32C株の植物生育促進効果への莢膜多糖(CPS)の関与(共生・相互作用,口頭発表)
西澤 智康; 箱山 雅生; 浦口 晋平; 作田 庄平; 藤原 徹; 磯部 一夫; 大塚 重人; 妹尾 啓史
日本微生物生態学会講演要旨集, 2013

3-1-13 水田土壌脱窒細菌Azoarcus KH32C株の接種による低窒素条件下での植物の生育促進(3-1 土壌生物の生態と機能,2013年度名古屋大会)
西澤 智康; 箱山 雅生; 浦口 晋平; 角田 洋子; 磯部 一夫; 大塚 重人; 作田 庄平; 藤原 徹; 妹尾 啓史
日本土壌肥料学会講演要旨集, 2013

S03-2 Multiple functions of denitrifying bacterium Azoarcus sp. KH32C in rice paddy soil as revealed by whole genome analysis(Session 3 Microbial community, function and nitrogen dynamics,Symposium session)
Nishizawa,Tomoyasu; Fujiii,Daichi; Quan,Aihua; Tago,Kanako; Ishii,Satoshi; Oshima,Kenshiro; Hattori,Masahira; Uraguchi,Shinnpei; Fujiwara,Toru; Isobe,Kazuo; Otsuka,Shigeto; Senoo,Keishi
日本微生物生態学会講演要旨集, 19 Sep. 2012

水田土壌脱窒細菌Azoarcus sp.KH32C株のゲノム解析から見出されたポリケチド合成遺伝子の系統解析
西澤智康; 藤井大地; 多胡香奈子; 石井聡; 大塚重人; 大島健志朗; 服部正平; 妹尾啓史
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2012

N_2O除去脱窒菌を利用した有機質ペレット肥料からのN_2O発生低減化(2012年度土壌微生物学会講演要旨)
全 愛花; 西澤 智康; 多胡 香奈子; 石井 聡; 磯部 一夫; 大塚 重人; 妹尾 啓史
土と微生物, 2012

水田土壌脱窒細菌Azoarcus KH32C株の接種による低窒素条件下でのイネ初期生育の促進
西澤智康; 藤井大地; 浦口晋平; 藤原徹; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌微生物学会講演要旨集, 2012

N₂O除去脱窒菌を利用した有機質ペレット肥料からのN₂O発生低減化
全愛花; 西澤智康; 多胡香奈子; 石井聡; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌微生物学会講演要旨集, 2012

水田土壌細菌の脱窒機能遺伝子の検出と系統解析
甲斐文彬; 磯部一夫; 西澤智康; 上井佑介; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 2012

微生物を利用した農耕地からのN₂Oガス発生低減化の試み
全愛花; 西澤智康; 多胡香奈子; 石井聡; 甲斐文彬; 磯部一夫; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 2012

水田土壌から分離した脱窒細菌の接種による畑土壌からの一酸化二窒素(N₂O)ガス発生低減化
QUAN Aihua; 西澤智康; 多胡香奈子; 多胡香奈子; 石井聡; 石井聡; 磯部一夫; 大塚重人; 妹尾啓史
日本農芸化学会大会講演要旨集(Web), 2012

水田土壌脱窒細菌Azoarcus KH32C株の接種による低窒素条件下でのイネ初期生育の促進(2012年度土壌微生物学会講演要旨)
西澤 智康; 藤井 大地; 浦口 晋平; 藤原 徹; 磯部 一夫; 大塚 重人; 妹尾 啓史
土と微生物, 2012

メタゲノム解析から明らかにされた三宅島初成土壌微生物生態系の遷移(S02土壌環境変動を探るメタゲノミクスのアプローチ,シンポジウムセッション)
藤村玲子; 金錫元; 佐藤嘉則; 須田亙; 西澤智康; 大島健志朗; 服部正平; 上條隆志; 太田寛行
日本微生物生態学会講演要旨集, 2012

P6-8 土壌水分含量が畑地土壌のin vitro脱窒活性に及ぼす影響の解析(S6.N_2O生成と消去に関わる微生物研究の最前線-遺伝子解析からN_2O発生抑制まで-,6.土壌生物,2012年度鳥取大会)
太田 寛行; 新美 洋; 橋本 知義; 梅津 昌史; 佐藤 嘉則; 西澤 智康; 小松崎 将一; 金子 信博
日本土壌肥料学会講演要旨集, 2012

N₂O還元型脱窒細菌Azoarcus属KH32C株のゲノム解析
西澤智康; 多胡香奈子; 石井聡; 藤井大地; 大塚重人; 大島健志朗; 服部正平; 妹尾啓史
日本ゲノム微生物学会年会要旨集, 2012

三宅島2000年噴火堆積物から分離された鉄酸化細菌Leptospirillum ferrooxidans C2‐3株のゲノム解析
藤村玲子; 佐藤嘉則; 細川研; 西澤智康; 大島健志朗; 服部正平; 上條隆志; 太田寛行
土と微生物, 01 Nov. 2011

水田土壌脱窒細菌Azoarcus KH32C株の接種によるイネの低窒素栽培
西澤智康; 藤井大地; 浦口晋平; 藤原徹; 多胡香奈子; 大島健志朗; 服部正平; 石井聡; 大塚重人; 妹尾啓史
土と微生物, 01 Nov. 2011

亜酸化窒素還元脱窒菌Azoarcus属KH32C株の全ゲノム解析と窒素固定エンドファイトBH72株,芳香族化合物分解脱窒菌EbN1株との比較ゲノム
西澤智康; 多胡香奈子; 大島健志朗; 服部正平; 石井聡; 大塚重人; 妹尾啓史
日本微生物生態学会講演要旨集, 08 Oct. 2011

Pseudogulbenkiania属脱窒細菌の比較ゲノム解析
石井聡; 多胡香奈子; 大島健志朗; 西澤智康; 大塚重人; 服部正平; 妹尾啓史
日本微生物生態学会講演要旨集, 08 Oct. 2011

水田土壌脱窒細菌Azoarcus sp.KH32C株の全ゲノム解析から見えてきた水田土壌生態系への適応メカニズム
西澤智康; 多胡香奈子; 大島健志朗; 石井聡; 大塚重人; 服部正平; 妹尾啓史
日本土壌肥料学会講演要旨集, 08 Aug. 2011

6-31 Relationship between development of bacterial community and vegetation succession on early soil ecosystem in the Island of Miyake, Japan　　　　　　　　　　　　　　　
郭, 永; 藤村, 玲子; 細川, 研; 佐藤, 嘉則; 西沢, 智康; 上條, 隆志; 太田, 寛行
Abstracts of the meeting, the Society of the Science of Soil and Manure, Aug. 2011

糸状菌細胞に内生する細菌の存在とその検出法
佐藤嘉則; 成澤才彦; 西澤智康; 小松﨑将一; 太田寛行
土と微生物, Apr. 2011

分子生態学的な微生物解析手法の土壌肥料研究への活用
浅川晋; 長岡一成; 関口博之; 浦嶋泰文; 須賀有子; 西澤智康; 太田寛行; 山崎真嗣; 妹尾啓史
日本土壌肥料学雑誌, Apr. 2011

不耕起が落葉腐生菌の多様性に与える影響
MIURA TOSHIKO; KANEKO NOBUHIRO; NAKAMORI TAIZO; TSUZURA HO; NISHIZAWA TOMOYASU
日本生態学会大会講演要旨集, 08 Mar. 2011

Functional Single Cell分離法を用いた水田土壌脱窒細菌の群集構造解析 従来の分離法を用いた解析との比較
上井佑介; 西澤智康; 多胡香奈子; 石井聡; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 2011

N₂生成型脱窒細菌を利用した畑土壌からのN₂Oガス発生低減化の試み
全愛花; 西澤智康; 多胡香奈子; 石井聡; 大塚重人; 妹尾啓史
日本土壌肥料学会講演要旨集, 2011

有機系銅剤ノニルフェノールスルホン酸銅(NPS-Cu)負荷による土壌細菌/アーキアおよびアンモニア酸化菌の群集構造解析
河村明寛; 山田桂衣; 西澤智康; 佐藤嘉則; 太田寛行
日本微生物生態学会講演要旨集, 2011

霞ケ浦(西浦・北浦)に発生したシアノバクテリアMicrocystisの遺伝生態学的解析
菅原将太; 山崎由紀子; 村上諒; 二渡弘貴; 高根澤陽; 西澤智康; 原田健一; 朝山宗彦; 白井誠
日本微生物生態学会講演要旨集, 2011

新奇糸状性シアノバクテリアABRG5-3株の特徴付け
高根澤陽; 西澤智康; 花見知世; 朝山宗彦; 原田健一; 白井誠
日本微生物生態学会講演要旨集, 2011

シアノバクテリアMicrocystisにおける新規なNRPS/PKS融合遺伝子クラスターの解析
山崎由紀子; 二村絢奈; 高根澤陽; LI Chenyao; 西澤明人; 西澤智康; 朝山宗彦; 原田健一; 白井誠
日本分子生物学会年会プログラム・要旨集(Web), 2011

糸状性シアノバクテリアLimnothrix/Pseudanabaena sp.ABRG5-3株の分子特徴付け
朝山宗彦; 高根澤陽; 西澤智康; 白井誠
日本分子生物学会年会プログラム・要旨集(Web), 2011

環流土壌系を用いた硝化菌の動態解析 硝化アーキアの働きを考える
太田寛行; 山田桂衣; 河村明寛; 安田智昭; 佐藤嘉則; 西澤智康
日本土壌肥料学会講演要旨集, 2011

不耕起栽培畑地土壌における微生物多様性の解析 : 特定サイトでの7年間の調査(2011年度大会一般講演要旨)
河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
土と微生物, 2011

P-85 Analysis of bacterial community in early stage of soil ecosystem after the 2000 volcanic eruption in Miyake Island, Japan(Poster Session) :
GUO,YONG; FUJIMURA,REIKO; HOSOKAWA,KEN; SATO,YOSHINORI; NISHIZAWA,TOMOYASU; KAMIJO,TAKASHI; OHTA,HIROYUKI
23 Nov. 2010

P-200 Effect of Mimicking Microgravity on the Swarming Motility and Gene Expression of Pseudomonas aeruginosa Strains on the Agar Medium(Poster Session)
SATO,SHINYA; NISHIZAWA,TOMOYASU; SENOO,KEISHI; INOUE,TETSUYOSHI; OHTA,HIROYUKI
日本微生物生態学会講演要旨集, 23 Nov. 2010

SB-3 Denitrifiers Responsible for Low Emission of N_2O in Paddy Soils : Identification, Isolation, and Application to Mitigate N_2O Emission from Upland Soils(Nitrogen dynamics and microbial ecology in soil-plant ecosystem : Towards mitigation of N2O emission from soil environments,Symposium B)
SENOO,KEISHI; TAGO,KANAKO; ISHII,SATOSHI; NISHIZAWA,TOMOYASU; OTSUKA,SHIGETO
日本微生物生態学会講演要旨集, 23 Nov. 2010

P-130 Thinking about ecological species and ecotype of bacteria(Poster Session)
OTA,HIROYUKI; NISHIZAWA,TOMOYASU; FUJIMURA,REIKO; SATO,YOSHINORI
日本微生物生態学会講演要旨集, 23 Nov. 2010

P6-1 Characterization of soil microbial community in the early development of soil ecosystem in Miyake Island, Japan　　　　　　　　　　　　　　　
郭, 永; 藤村, 玲子; 細川, 研; 佐藤, 嘉則; 西沢, 智康; 上條, 隆志; 太田, 寛行
Abstracts of the meeting, the Society of the Science of Soil and Manure, Sep. 2010

畑地土壌におけるCryptococcus属真核微生物の分布と亜酸化窒素生成活性(2010年度大会一般講演要旨)
赤池 恵; 佐藤 嘉則; 西澤 智康; 昭日 格図; 太田 寛行
土と微生物, 2010

ノニルフェノールスルホン酸銅環流土壌中におけるアンモニア酸化菌の動態解析(2010年度大会一般講演要旨)
西澤 智康; 安田 智昭; 太田 寛行
土と微生物, 2010

畑地土壌におけるCryptococcus属真核微生物の分布と亜酸化窒素生成活性
赤池恵; 佐藤嘉則; 西澤智康; 昭日格図; 太田寛行
日本土壌微生物学会講演要旨集, 2010

不耕起栽培畑地土壌における微生物群集の分子生態学的解析
河村明寛; 西澤智康; 小松崎将一; 金子信博; 太田寛行
日本土壌微生物学会講演要旨集, 2010

分子生態学的な微生物解析手法の土壌肥料珊究への活用 不耕起草生栽培が畑土壌の微生物群集に及ぼす影響
西澤智康; 太田寛行
日本土壌肥料学会講演要旨集, 2010

横須賀のため池轡堰に発生したアオコに関する遺伝生態学的解析
星野彩子; 西澤智康; 高根澤陽; 朝山宗彦; 根上泰子; 村田浩一; 原田健一; 白井誠
日本微生物生態学会講演要旨集, 2010

ノニルフェノールスルホン酸銅環流土壌中における硝化菌の動態解析
山田桂衣; 河村明寛; 安田智昭; 佐藤嘉則; 西澤智康; 太田寛行
日本微生物生態学会講演要旨集, 2010

霞ケ浦に発生したシアノバクテリアMicrocystisの遺伝生態学的解析
村上諒; 二渡弘貴; 西澤智康; 朝山宗彦; 原田健一; 白井誠
日本微生物生態学会講演要旨集, 2010

Functional Single Cell分離法を用いた水田土壌の脱窒細菌の群集構造解析
上井佑介; 多胡香奈子; 石井聡; 西澤智康; 大塚重人; 妹尾啓史
日本微生物生態学会講演要旨集, 2010

新奇糸状性シアノバクテリアABRG5-3株の遺伝学及び生理学的特徴づけ
高根澤陽; 西澤智康; 花見知世; 平野恵理子; 三浦隆匡; 渡辺優子; 原田健一; 朝山宗彦; 白井誠
生化学, 2010

ノニルフェノールスルホン酸銅環流土壌中におけるアンモニア酸化菌の動態解析
西澤智康; 安田智昭; 太田寛行
日本土壌微生物学会講演要旨集, 2010

半糸状性有用藍藻ABRG5-3の特徴付けと宿主発現ベクター系の構築
朝山宗彦; 西澤智康; 花見知世; 平野恵理子; 高根澤陽; 太田寛行; 白井誠
日本農芸化学会大会講演要旨集, 2010

霞ケ浦に発生したアオコの微生物群集構造解析
西澤智康; 二渡弘貴; 朝山宗彦; 原田健一; 白井誠
藻類, 2010

1A-14 Characterization of endobacterial communities in dark-sepate endophytic fungus, Veronaeopsis simplex associated with biocontrol mechanisms of Fusarium disease of Chinese Cabbage(Oral Session) :
KHASTINI RIDA; SATO YOSHINORI; NISHIZAWA TOMOYASU; OHTA HIROYUKI; NARISAWA KAZUHIKO
日本微生物生態学会講演要旨集, 2010

P-26 Nitrous oxide-producing activity of fungi isolated from no-tilled upland soils(Poster Session)
AKAIKE MEGUMI; SATO YOSHINORI; NISHIZAWA TOMOYASU; KOMATSUZAKI MASAKAZU; OHTA HIROYUKI
日本微生物生態学会講演要旨集, 2010

P-76 T-RFLP profiling of soil archaeal community in no-tilled upland soils(Poster Session)
KAWAMURA AKIHIRO; NISHIZAWA TOMOYASU; KOMATSUZAKI MASAKAZU; KANEKO NOBUHIRO; OHTA HIROYUKI
日本微生物生態学会講演要旨集, 2010

不耕起栽培畑地土壌における微生物群集の分子生態学的解析(2010年度大会一般講演要旨)
河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
土と微生物, 2010

オカボおよびダイズの不耕起栽培におけるカバークロップ利用とミミズ導入の効果
小松崎将一; 西澤智康; 平出圭司郎; 太田寛行; 金子信博; 三浦季子
農作業研究, 2009

高効率プロモーターを有する藻発現ベクターの構築と利用
朝山宗彦; 平野恵理子; 花見知世; 堀江良尚; 西澤智康; 白井誠
日本分子生物学会年会講演要旨集, 2009

細菌についての生態学的種を考える:この数年の展開をめぐって
太田寛行; 西澤智康; 佐藤嘉則; 藤村玲子; 藤村玲子
日本微生物生態学会講演要旨集, 2009

霞ケ浦に発生した毒素生産性シアノバクテリアMicrocystisの遺伝学的解析
二渡弘貴; 西澤智康; 野口貴彦; 原田健一; 朝山宗彦; 白井誠
日本微生物生態学会講演要旨集, 2009

ノニルフェノールスルホン酸銅がアンモニア酸化バクテリア・アーキアに与える影響
安田智昭; 梅津昌史; 鶴田和人; 西澤智康; 佐藤嘉則; 高原義治; 太田寛行
日本微生物生態学会講演要旨集, 2009

模擬微小重力条件におけるPseudomonas aeruginosaのpilA転写解析
佐藤慎哉; 進士真也; 小里郁美; 西澤智康; 井上哲圭; 太田寛行
日本微生物生態学会講演要旨集, 2009

三宅島火山灰堆積物における微生物の鉄代謝
細川研; 郭永; 藤村玲子; 佐藤嘉則; 西澤智康; 難波謙二; 上條隆志; 大田寛行
日本微生物生態学会講演要旨集, 2009

アクチノファージR4のφ部位特異的組換え酵素遺伝子周辺遺伝子の解析
米津篤人; 三浦隆匡; 佐野夏枝; 原田千津子; 西澤智康; 朝山宗彦; 高橋秀夫; 白井誠
日本分子生物学会年会講演要旨集, 2009

シアノバクテリアMicrocystis属におけるホスホパンテテイントランスフェラーゼの遺伝・生化学的解析
渡辺優子; 西澤明人; 西澤智康; 朝山宗彦; 原田健一; 白井誠
日本分子生物学会年会講演要旨集, 2009

P-83 環境保全型農法を継続した畑地土壌における真核微生物の群集構造解析(ポスター発表)
西澤 智康; 河村 明寛; 佐藤 嘉則; 小松崎 将一; 金子 信博; 太田 寛行
日本微生物生態学会講演要旨集, 2009

P-63 環境保全型農法と土壌細菌・アーキア群集 : ミミズ導入とカバークロップの影響(ポスター発表)
河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
日本微生物生態学会講演要旨集, 2009

1A-17(P-86) 地表部の撹乱が地下圏微生物にどのような影響をおよぼすのか(口頭発表+ポスター発表)
永翁 一代; 勝山 千恵; 近藤 菜穂; 村上 正志; 西澤 智康; 太田 寛行; 金子 信博; 加藤 憲二
日本微生物生態学会講演要旨集, 2009

S6-17 末端制限断片(T-RFs)データによる畑地土壌微生物多様性解析 : 複数の解析手法を用いた評価方法の検討(S6.分子生態学的解析手法を研究室のルーチンワークに!パート2それによって見えるもの、見たいもの,6.土壌生物,2009年度京都大会)
西澤 智康; 石井 真英; 小松崎 将一; 金子 信博; 太田 寛行
日本土壌肥料学会講演要旨集, 2009

T-RELPプロファイルによる畑地土壌微生物群集の解析(2009年度大会一般講演要旨)
西澤 智康; 石井 真英; 梅津 昌史; 昭日 格図; 小松崎 将一; 金子 信博; 太田 寛行
土と微生物, 2009

10-198 Identification of nitrogen fixing bacteria in recent volcanic deposits of Miyake-Jima by stable isotope probing(Microbial community analysis)
Sugawara,Tomonori; Sato,Yoshinori; Fujimura,Reiko; Nagasawa,Toshiki; Hosokawa,Ken; Nishizawa,Tomoyasu; Nanba,Kenji; Kamijo,Takashi; Ohta,Hiroyuki
日本微生物生態学会講演要旨集, Nov. 2008

04-078 Isolation of iron-oxidizing bacteria by chemostat from Miyake-jima volcanic ash deposits(Soil ecosystem)
Hosokawa,Ken; Sato,Yoshinori; Sugawara,Tomonori; Fujimura,Reiko; Nanba,Kenji; Kamijo,Takashi; Nishizawa,Tomoyasu; Ohta,Hiroyuki
日本微生物生態学会講演要旨集, Nov. 2008

三宅島2000年噴火堆積物の初成土壌形成と細菌生態系(2008年度大会一般講演要旨)　　　　　　　　　　　　　　　
藤村, 玲子; 佐藤, 嘉則; 長澤, 俊樹; 細川, 研; 西澤, 智康; 難波, 謙二; 加藤, 拓; 片山, 葉子; 上條, 隆志; 東, 照雄; 太田, 寛行
土と微生物, Oct. 2008

T-RFLP解析による土壌微生物群集の評価 : ノニルフェノールスルホン酸銅が及ぼす影響(2008年度大会一般講演要旨)
安田 智昭; 梅津 昌史; 鶴田 和人; 西澤 智康; 佐藤 嘉則; 高原 義治; 太田 寛行
土と微生物, 2008

土壌微生物群集の定量的T-RFLP解析 : 窒素負荷量の異なる土壌について(2008年度大会一般講演要旨)
梅津 昌史; 佐藤 嘉則; 西澤 智康; 新美 洋; 橋本 知義; 小松崎 将一; 太田 寛行
土と微生物, 2008

不耕起栽培における糸状菌多様性の評価(2008年度大会一般講演要旨)
昭日 格図; 西澤 智康; 佐藤 嘉則; 小松崎 将一; 太田 寛行
土と微生物, 2008

T-RFLPデータベースによる畑地土壌細菌群集構造の解析
石井真英; 昭日格図; 昭日格図; 西澤智康; 小松崎将一; 太田寛行
日本土壌肥料学会講演要旨集, 2008

不耕起栽培における糸状菌多様性の評価
昭日格図; 昭日格図; 西澤智康; 佐藤嘉則; 小松崎将一; 太田寛行
日本土壌微生物学会講演要旨集, 2008

定量的T-RFLP解析法による畑地土壌微生物の多様性評価
西澤智康; 小松崎将一; 金子信博; 太田寛行
日本土壌微生物学会講演要旨集, 2008

T-RFLP解析による土壌微生物群集の評価:ノニルフェノールスルホン酸銅が及ぼす影響
安田智昭; 梅津昌史; 鶴田和人; 西澤智康; 佐藤嘉則; 高原義治; 太田寛行
日本土壌微生物学会講演要旨集, 2008

土壌微生物群集の定量的T-RFLP解析:窒素負荷量の異なる土壌について
梅津昌史; 佐藤嘉則; 西澤智康; 新美洋; 橋本知義; 小松崎将一; 太田寛行
日本土壌微生物学会講演要旨集, 2008

有機物連用土壌における亜酸化窒素発生に関わる微生物群集構造の解析
梅津昌史; 佐藤嘉則; 西澤智康; 新美洋; 橋本知義; 成澤才彦; 小松崎将一; 太田寛行
日本微生物生態学会講演要旨集, 2008

シアノバクテリアMicrocystis属におけるホスホパンテテイントランスフェラーゼの遺伝・生化学的解析
渡辺優子; 西澤明人; 西澤智康; 朝山宗彦; 原田健一; 白井誠
生化学, 2008

山間水域より分離した新規光合成微生物の性格付け
西澤智康; 花見知世; 三浦隆匡; 渡辺優子; 小松崎将一; 太田寛行; 白井誠; 朝山宗彦
日本農芸化学会関東支部講演要旨集, 2008

三宅島2000年間噴火堆積物の初成土壌形成と細菌生態系
藤村玲子; 藤村玲子; 佐藤嘉則; 長澤俊樹; 細川研; 西澤智康; 難波謙二; 加藤拓; 片山葉子; 上條隆志; 東照雄; 太田寛行; 太田寛行
日本土壌微生物学会講演要旨集, 2008

02-033 Fungal community and nitrous oxide emission in no-tillage upland soils(Soil ecosystem)
Zhaori Getu; Nishizawa Tomoyasu; Sato Yoshinori; Tsuruta Kazuto; Komatsuzaki Masakazu; Ohta Hiroyuki
日本微生物生態学会講演要旨集, 2008

10-195 Effect of bactericidal copper nonylphenol sulfonate on the soil microbial communities : A model using a soil percolation column(Microbial community analysis)
Yasuda Tomoaki; Umezu Masahumi; Tsuruta Kazuto; Nishizawa Tomoyasu; Sato Yoshinori; Takahara Yoshiharu; Ohta Hiroyuki
日本微生物生態学会講演要旨集, 2008

11-201 Microbial community analysis of upland field soils by a database search of the terminal-restriction fragments (T-RFs)(Microbial community analysis)
Nishizawa Tomoyasu; Komatsuzaki Masakazu; Kaneko Nobuhiro; Ohta Hiroyuki
日本微生物生態学会講演要旨集, 2008

07-133 Influence of geophagous invertebrate(s) on microbial community in forest soil(Soil ecosystem)
Kondo Naho; Katsuyama Chie; Nagaosa Kazuyo; Nishizawa Tomoyasu; Kaneko Nobuhiro; Kato Kenji
日本微生物生態学会講演要旨集, 2008

06-105 Analysis of upland soil bacterial community by T-RFLP informatics(Microbial community analysis)
Ishii Masahide; Nishizawa Tomoyasu; Komatsuzaki Masakazu; Kaneko Nobuhiro; Ohta Hiroyuki
日本微生物生態学会講演要旨集, 2008

Soil carbon accumulation rate at Tomakomai Experimental Forest of Hokkaido University
Kaneko Nobuhiro; Iwashima Noriko; Toyota Ayu; Fujimaki Reiji; Kawaguchi Tatsuya; Hiura Tsutom; Murakami Masashi; Tayasu Ichiro; Ohta Hiroyuki; Nishizawa Tomoyasu; Masunaga Tsugiyuki
The Japanese Forest Society Congress, 2008

定量的T-RFLP解析法による畑地土壌微生物の多様性評価(2008年度大会一般講演要旨)
西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
土と微生物, 2008

シアノバクテリアにおける新規なポリケタイド合成遺伝子の解析
LI Chenyao; 西澤明人; 西澤智康; 朝山宗彦; 原田健一; 白井誠
生化学, 2007

不耕起栽培における糸状菌の動態について
昭日格図; 西澤智康; 佐藤嘉則; 小松崎將一; 太田寛行
日本微生物生態学会講演要旨集, 2007

新規光合成微生物の単離と分離菌の性格付け
西澤智康; 小松崎将一; 太田寛行; 白井誠; 朝山宗彦
日本農芸化学会関東支部講演要旨集, 2007

Microcystis aeruginosa K-139におけるphosphopantetheinlyl transferase(PPTase)の同定と機能解析
渡辺優子; 西澤明人; 西澤智康; 朝山宗彦; 白井誠
生化学, 2007

PA-29 Diversity of soil microbial communities assessed by PCR-amplified T-RFLP analysis : effects of tillage treatments(Agricultural ecosystem,Poster presentation A) :
Nishizawa Tomoyasu; Komatsuzaki Masakazu; Kaneko Nobuhiro; Ohta Hiroyuki
日本微生物生態学会講演要旨集, 2007

B-09 Highly Conserved Organization of Microcystin Synthetase Gene Clusters among the Genus Microcystis(Genetic analysis,(2)Oral presentation)
Nishizawa,Tomoyasu; Nishizawa,Akito; Noguchi,Takahiko; Harada,Ken-ichi; Asayama,Munehiko; Shirai,Makoto
日本微生物生態学会講演要旨集, 27 Oct. 2006

高度好熱菌(Thermus thermophilus)の緊縮制御機構の生理・生化学的解析
笠井光治; 西澤智康; 高橋公咲; 保坂毅; 越智幸三
日本農芸化学会大会講演要旨集, 2006

高温菌Thermus thermophilusにおける緊縮制御
越智幸三; 笠井光治; 西澤智康; 高橋公咲; 保坂毅; 青木裕之
極限環境微生物学会誌, 2006

C-220 シアノバクテリアが生産するペプチドに関わる合成酵素遺伝子の研究(遺伝子解析など-3、群集構造解析-4,ポスター発表)
西澤,智康; 上田,明子; 朝山,宗彦; 藤井,清永; 原田,健一; 白井,誠
日本微生物生態学会講演要旨集, 12 Nov. 2000

A-05 ミクロシスチン合成遺伝子に基づく毒素生産性ラン藻Microcystis属の遺伝学的識別(遺伝子解析など-2,口頭発表)
西澤,智康; 朝山,宗彦; 白井,誠
日本微生物生態学会講演要旨集, 12 Nov. 2000

Genetic analysis of the genes encodeing the enzyme of cyclic peptide microcystin syntesis in cyanobacterium
NISHIZAWA Tomoyasu; UEDA Akiko; ASAYAMA Munehiko; FUJII Kiyonaga; HARADA Kenichi; SHIRAI Makoto
日本分子生物学会年会プログラム・講演要旨集, 01 Dec. 1998

Investigating Endobacteria that Thrive Within Mucoromycota. In: Martin, F., Uroz, S. (eds) Microbial Environmental Genomics (MEG). Methods in Molecular Biology, vol 2605. Humana, New York, NY.
Desirò; A.; Y. Takashima; G. Bonito; T. Nishizawa; K. Narisawa; P. Bonfante, Joint work
Humana, New York, NY, 16 Dec. 2022, [Reviewed]
9781071628706

Current Technology and Innovation with Microbes in Nature　　　　　　　　　　　　　　　
太田寛行・佐藤嘉則・西澤智康, Joint work
シーエムシー出版, 11 Apr. 2022
9784781316314

実践土壌学シリーズ３ 土壌生化学　　　　　　　　　　　　　　　
境 雅夫; 西澤智康, Contributor
朝倉書店, 05 Feb. 2019
9784254435733

実践土壌学シリーズ１ 土壌微生物学　　　　　　　　　　　　　　　
西澤智康, Contributor
朝倉書店, 10 Aug. 2018
9784254435719

土のひみつ 食料・環境・生命　　　　　　　　　　　　　　　
西澤智康, Contributor
朝倉書店, 10 Sep. 2015
9784254400236

難培養微生物研究の最前線Ⅲ―微生物の生き様に迫り課題解決へ―　　　　　　　　　　　　　　　
太田寛行; 佐藤嘉則; 西澤智康, Joint work
シーエムシー出版, 07 Aug. 2015
9784781310817

土壌微生物実験法 第3版（日本土壌微生物学会編）　　　　　　　　　　　　　　　
西澤智康, Contributor
養賢堂, 20 Jun. 2013
9784842505169

Photosynthesis: Theory and Applications in Energy, Biotechnology and Nanotechnology　　　　　　　　　　　　　　　
Nishizawa; T.; M. Asayama; M. Shirai, Contributor
Nova Science Publishers, Inc., 29 May 2009, [Reviewed]
9781606927199

Adaptive Gene Regulations-from Microorganisms to Organelles　　　　　　　　　　　　　　　
Nishizawa; T.; A. Nishizawa; M. Shirai, Contributor
Research Signpost, Dec. 2008, [Reviewed]
9788130802664

霞ケ浦（北浦）の湖底堆積物における窒素循環系微生物群集構造解析　　　　　　　　　　　　　　　
正田岳志; 中里亮治; 西澤智康
公開シンポジウム 霞ヶ浦流域研究2024（GLEC水圏環境ﾌｨｰﾙﾄﾞｽﾃｰｼｮﾝ・オンライン）, Mar. 2024
20240303

霞ヶ浦（北浦）および河川流域における薬剤耐性細菌の動態解析　　　　　　　　　　　　　　　
早川美久; 中里亮治; 西澤智康
公開シンポジウム 霞ヶ浦流域研究2024（GLEC水圏環境ﾌｨｰﾙﾄﾞｽﾃｰｼｮﾝ・オンライン）, Mar. 2024
20240303

カバークロップと微生物資材を利用した有機水稲栽培における水田土壌管理と水稲収量との関係　　　　　　　　　　　　　　　
加来嵩時・武藤光輝・高嶋尚哉・菅井 純・遠山佳甫・迫田 翠・浅木直美・伊沢 剛・西澤智康・小松﨑将一
第24回 日本有機農業学会(大阪)大会（摂南大学 枚方キャンパス）, Dec. 2023
20231209, 20231210

Identification and distribution of fruiting body-forming ectomycorrhizal fungi ,on soil surface of the Burial jars for children, the Sannai Maruyama Site　　　　　　　　　　　　　　　
〇Rirei NAGAO; Takeru TODOROKI; Kiyomi YAMADA; Juni SASAKI; Takeshi ISHIZAKI; Yoshinori SATO; Tomoyasu NISHIZAWA
日本文化財科学会第40回記念大会（奈良、奈良県立なら歴史芸術文化村）, 21 Oct. 2023
20231020, 20231022

耕起から不耕起への転換が畑地土壌の窒素動態に及ぼす影響　　　　　　　　　　　　　　　
遠山佳甫; 淺木直美; 迫田 翠; 郭 永; 西澤智康
日本土壌肥料学会2023年度愛媛大会（愛媛大学 城北キャンパス）, 12 Sep. 2023
20230912, 20230913

霞ヶ浦（北浦）湖底堆積物における薬剤耐性細菌の推定　　　　　　　　　　　　　　　
早川 美久; 郭 永; 迫田 翠; 正田 岳志; 中里 亮治; 西澤 智康
日本土壌微生物学会2023年度大会（千葉大学 松戸キャンパス）, 10 Jun. 2023, 日本土壌微生物学会
20230610, 20230611

Effect of no-tillage on SOM accumulation and biological properties of Andosols from a long-term experiment in Japan　　　　　　　　　　　　　　　
Aduhene-Chinbuah; J; S. Sughihara; M. Komatsuzaki; T. Nishizawa; H. Tanaka
8th International Symposium on Soil Organic Matter (SOM 2022, Seoul, Korea), Jun. 2022, Korean Society of Soil Science and Fertilizer (KSSSF)
20220626, 20220630

農学的な切り口で温室効果ガスの発生と消費を考えてみる,ー微生物が、どのように気候変動に影響を与えているのか？ー　　　　　　　　　　　　　　　
西澤智康
第７回 茨城大学カーボンニュートラルオープンセミナー（オンライン開催）, 10 Nov. 2021, 茨城大学「2050年カーボンニュートラル」連続講演会タスクフォース, [Invited]
20211110, 20211110

Microbial interactions of endobacteria coexisted within the filamentous fungus cells　　　　　　　　　　　　　　　
Yong GUO; Yusuke TAKASHIMA; Tomoyasu NISHIZAWA
第94回 日本細菌学会総会（オンライン開催）, 23 Mar. 2021, [Invited]
20210323, 20210325

（Presentation in Symposium）,Change of structure and dynamics of microbe community in the 2000 year volcanic ash　　　　　　　　　　　　　　　
Hiroyuki Ohta,Tomoyasu Nishizawa
日本生態学会 第68回大会（岡山大会・オンライン）, 17 Mar. 2021, [Invited]
20210317, 20210321

土壌団粒構造と土壌プロセス2 畑地土壌団粒における微生物群集の構造・動態変化 農地土壌管理が微生物性に与えるインパクト　　　　　　　　　　　　　　　
西澤智康; 太田寬行
日本土壌肥料学会2019年度大会（静岡、静岡大学）, Sep. 2019, 日本土壌肥料学会

土壌団粒構造と土壌プロセス2,畑地土壌団粒における微生物群集の構造・動態変化 農地土壌管理が微生物性に与えるインパクト　　　　　　　　　　　　　　　
西澤智康・太田寬行
日本土壌肥料学会2019年度大会（静岡、静岡大学）, Sep. 2019, 日本土壌肥料学会

糸状菌ー内生細菌の微生物間相互作用現象の解明
西澤智康
第13回助成研究報告会（大阪、千里ライフサイエンスセンター）, 07 Jun. 2019, 公益財団法人発酵研究所, [Invited]

The distribution of antibiotic-resistant bacteria in eutrophic Lake Kasumigaura and neighboring agricultural fields
矢用もも; 平野明則; 松田祐輝; 上原研人; 今井達也; 島袋真; 黒田久雄; 太田寛行; 西澤智康
第17回世界湖沼会議（いばらき霞ヶ浦2018）分科会（つくば国際会議場）, Oct. 2018

Soil environmental condition and vegetation recovery of the abandoned cropland around Komado-shitsugen moor, Fukushima prefecture
坂上伸生・郭永・高島勇介・西澤智康・成澤才彦・渡邊眞紀子
第17回世界湖沼会議（いばらき霞ヶ浦2018）分科会（つくば国際会議場）, Oct. 2018

『農法の違いを微生物性解析で「見える化」』
西澤 智康
第三回土壌団粒勉強会（仙台、東北大学）, Sep. 2017

土壌層位形成過程や土壌団粒構造の微生物群集構造解析　　　　　　　　　　　　　　　
西澤 智康; 太田 寬行
環境微生物系学会合同大会2017（仙台、東北大学）, Aug. 2017

石人山古墳装飾石棺表面の微生物群集構造解析　　　　　　　　　　　　　　　
佐藤嘉則; 西澤智康; 小沼奈那美; 犬塚将英; 森井順之; 木川りか; 朽津信明
文化財保存修復学会第39回大会（石川、金沢歌劇座）, Jul. 2017

土壌生成プロセスにおける微生物の役割　　　　　　　　　　　　　　　
太田寬行; 西澤智康
日本土壌微生物学会 2015 年度大会（つくば）：学会シンポジウム「国際土壌年－土と微生物を考える」, May 2015

Plant growth promoting ability of Azoarcus sp. KH32C, a potent N₂O-mitigating denitrifier　　　　　　　　　　　　　　　
Tomoyasu Nishizawa; Ayaaki Kai; Hiroko Kakuta; Kazuo Isobe; Shigeto Otsuka; Tsuneo Hakoyama; Shimpei Uraguchi; Toru Fujiwara; Keishi Senoo
18th International Congress on Nitrogen Fixation, Oct. 2013

霞ヶ浦に発生したアオコの微生物群集構造解析　　　　　　　　　　　　　　　
西澤 智康; 二渡 弘貴; 朝山 宗彦; 原田 健一; 白井 誠
日本藻類学会第 34 回大会（つくば 2010）（茨城，筑波大学）, Mar. 2010

有毒アオコ群集の発生機構と動態解析　　　　　　　　　　　　　　　
西澤 智康
第3回公開シンポジウム「霞ヶ浦流域再生プロジェクト」, Oct. 2009, 茨城大学農学部

末端制限断片(T-RFs)データによる畑地土壌微生物多様性解析：複数の解析手法を用いた評価方法の検討　　　　　　　　　　　　　　　
西澤 智康; 石井 真英; 小松崎 将一; 金子 信博; 太田 寛行
日本土壌肥料学会2009年度京都大会（京都大学）, Sep. 2009, 日本土壌肥料学会 第3 部門

T-RFLPプロファイルによる畑地土壌微生物群集構造の解析　　　　　　　　　　　　　　　
西澤智康; 石井真英; 梅津昌史; 昭日格図; 小松崎将一; 金子信博; 太田寛行
日本土壌微生物学会2009年度大会（九州、福岡）, Jun. 2009

Microcystis株におけるミクロシスチン合成遺伝子構成の保存性　　　　　　　　　　　　　　　
西澤智康; 西澤明人; 野口貴彦; 原田健一; 朝山宗彦; 白井誠
第22回 日本微生物生態学会（東京、東京大学）, Oct. 2006

2014 - Present, American Society for Microbiology

2009 - Present, 日本土壌肥料学会

2009 - Present, 日本ゲノム微生物学会

2008 - Present, 日本土壌微生物学会

Jun. 2006 - Present, 日本微生物生態学会