2023年03月, 日本育種学会功労賞, 日本育種学会
国内学会・会議・シンポジウム等の賞

2004年, 日本育種学会奨励賞

Frame-shift mutation ofInCOmight cause early flowering of Japanese morning glory and might have contributed to northward expansion
Hiroaki Katsuyama; Takuro Ito; Kyousuke Ezura; Emdadul Haque; Atsushi Hoshino; Eiji Nitasaka; Michiyuki Ono; Shusei Sato; Sachiko Isobe; Hiroyuki Fukuoka; Nobuyoshi Watanabe; Tsutomu Kuboyama, Abstract

Japanese morning glory (Ipomoea nil), a short day plant, has been used for studying flowering times. Here, quantitative trait loci (QTL) analysis for days from sowing to flowering (DTF) of F₂betweenI. nilvar. Tokyo Kokei Standard (TKS) andI. hederacealine var. Q65, an early flowering variety, revealed four QTLs:QTL Ipomoea Flowering 1–4(qIF1–4). The position ofqIF3, which had the most significant effect among the four QTLs, corresponds with that ofI. nil(orI. hederacea)CONSTANS(InCO/IhCO) in the linkage map. There is a single-base In/Del in the coding sequence ofInCO/IhCO. The single-base deletion (SBD) causes a frame-shift mutation and loss of function in TKS allele (inco-1).I. nilaccessions bearinginco-1tend to flower early, similarly to rice varieties bearing the loss of function allele ofCOortholog,hd1. Consequently,inco-1was inferred to reduce DTF. This inferred effect ofinco-1corresponds with the effect of the TKS allele ofqIF3. Becauseinco-1is found exclusively in Asian accessions, the SBD ininco-1might have played an important role in the expansion of Japanese morning glories, originally native to the tropical regions of the Americas, into temperate Asia., Cold Spring Harbor Laboratory
2024年09月08日

中性子線を用いた突然変異育種への期待　　　　　　　　　　　　　　　
久保山 勉, 公益社団法人 農林水産・食品産業技術振興協会
JATAFFジャーナル, 2023年09月01日, ［招待有り］

PAPER Transcriptome profiling for pericarp browning during long‑term storage of intact lotus root (Nelumbo nucifera)　　　　　　　　　　　　　　　
Kanjana Worarad1 · Tomohiro Suzuki2 · Haruka Norii1 · Yuya Mochizuki1 · Takashi Ishii3 · Keiko Shinohara4 · Takao Miyamoto5 · Tsutomu Kuboyama1 · Eiichi Inoue, Springer
Plant Growth Regulation, 2021年08月11日, ［査読有り］

伝統野菜レンコン（蓮根）のブランド力強化ー高品質な国産レンコンの需要拡大と輸出に向けて　　　　　　　　　　　　　　　
井上栄一・久保山勉, 日本化学会
化学と工業, 2019年12月01日, ［招待有り］

HWA1- and HWA2-mediated hybrid weakness in rice involves cell death, reactive oxygen species accumulation, and disease resistance-related gene upregulation
Kumpei Shiragaki; Takahiro Iizuka; Katsuyuki Ichitani; Tsutomu Kuboyama; Toshinobu Morikawa; Masayuki Oda; Takahiro Tezuka
Plants, 2019年10月25日, ［査読有り］

植物の生殖隔離機構解明に向けて ―多様性と統一性―
手塚孝弘; 一谷勝之; 松本雄一; 何海; 木下哲; 宅見薫雄; 久保山勉
育種学研究, 2019年06月

ddRAD‐seq解析より得られたSNPに基づくレンコン品種識別法の開発
小林希美; 白澤健太; 堀井学; 篠原啓子; 澤田英司; 八城和敏; 樋口洋平; 石川祐聖; HAQUE E; 井上栄一; 久保山勉
育種学研究, 2018年03月25日

Genetic mapping of diagnostic markers for the Lg(2) locus conferring ligules in Triticum aestivum L. and derived from Aegilops tauschii Coss.
Y. Amagai; T. Kuboyama; N. Watanabe, It has been suggested that the non-allelic lg(2) and Lg(t) alleles, that confer liguleless phenotypes, are present in chromosome 2D. We hypothesized that the naturally occurring liguleless phenotype present in polyploid wheat was a typical example of loss of dominant duplicated alleles, a process that has been described as genetic diploidisation. In the present study, we obtained the base sequence of the candidate of Lg(2) gene through a "blastn" query with the upstream region of HvLG, the candidate gene sequence for the ligule locus in barley (Hordeum vulgare), and developed deletion markers of the Lg(2) locus from Ae. tauschii. We applied the deletion markers to locate the Lg(2) locus in hexaploid wheat. The F-2 progeny of ANK-33/SynW-5 segregated in a 3 liguled: 1 liguleless ratio. The Lg(2) gene was completely linked to markers G3489_2DL12del and G3489_2DL11del and segregated in a 1: 2: 1 ratio. Liguleless Mutant, a liguleless mutant of Aegilops tauchii, was determined by the dominant Lg(t) gene. In Ae. tauschii, the F-2 progeny of KU2126/Liguleless Mutant, marker Atlg_2DL19del segregated in a 1: 2: 1 ratio independently of the 3 liguleless: 1 liguled segregation. The results indicate that three deletion markers can be used to mark the Lg(2) locus in chromosome 2D of synthetic and hexaploid wheat., SPRINGER
EUPHYTICA, 2017年03月, ［査読有り］

Distribution of Hwc2-1, a causal gene of a hybrid weakness, in the World Rice Core collection and the Japanese Rice mini Core collection: its implications for varietal differentiation and artificial selection
Katsuyuki Ichitani; Satoru Taura; Muneharu Sato; Tsutomu Kuboyama, A pair of complementary genes, Hwc1-1 at HFVCI locus and Hwc2-1 at HWC2 locus, cause a weakness phenomenon in rice. For this study, we performed haplotype analysis around the HWC2 locus in two core collections comprising 119 accessions. We also examined reactions to phenol and Xanthomonas oryzae pv. oryzae (Xoo) Japanese race I. To elucidate the genetic relations among all accessions, we analyzed their banding patterns of 40 Indel markers covering the rice genome. The classification by Indel markers was almost consistent with that using 4,357 SNPs. The testcross with Hwc1-1 carrier indicated that 37 accessions carried Hwc2-1 allele, whereas 82 carried hwc2-2 allele. Strong association between HWC2 and Ph genes was observed. Based on 14 DNA markers around HWC2 locus and Ph genotype, the 119 accessions were divided into 50 haplotypes. To examine the HWC2 candidate chromosomal region specifically, the 'haplotype group' characterized by the six DNA markers closely linked with HWC2 were analyzed. Hwc2-1 carriers had the same haplotype group. Some hwc2-2 haplotype groups were associated with resistance against the Xoo race. The relation between varietal differentiation and haplotypes around the HWC2 locus was discussed, along with its breeding significance., JAPANESE SOC BREEDING
BREEDING SCIENCE, 2016年12月, ［査読有り］

Microsatellite mapping of the gene conferring compact spike in Japanese “Gumbai” landraces of common wheat (Triticum aestivum L.)
Y. Amagai; T. Kuboyama; N. Watanabe, The "Gumbai" wheat landraces of are a distinctive group of Japanese common wheat landraces. They originated in the Yamanashi region of Japan and are characterized by compact spikes, which were called "Gumbai" in the Japanese language. It is well known that the spikes of club wheat (Triticum compactum Host, 2n = 6x = 42, BBAADD genome) are governed by the dominant allele of the compactum (C) locus. We were interested to determine if the compact spike trait in "Gumbai" landraces is controlled either by the gene on chromosome 2D or by an orthologous gene. Five F-2 hybrid populations of 'Novosibirskaya 67' (N67)/"Gumbai" landraces segregated in 3 compact spike: 1 normal ratios. We tentatively designated the gene C (g) (Compact spike of Gumbai) for "Gumbai" landraces. The C (g) gene was not allelic to the C locus on chromosome 2D, or the Cp locus on chromosome 5A. In an F-2 population of N67/'Nakate Gumbai', there was linkage among C (g) and microsatellite loci Xgwm47, Xhbg410 and Xhbg440. Deletion mapping, showed that Xhbg410 and Xhbg440 were located across bins 2BL-7 0.48-0.69 and 2BL-1 0.69-0.89. These results indicated that the C (g) gene governing compact spike lies near breakpoint 0.69 in chromosome arm 2BL. The C (g) gene was located in the orthologous region of a spike compactness QTL in chromosome arm 2AL of wild emmer., SPRINGER
Euphytica, 2016年06月, ［査読有り］

Y. Amagai, P. Martinek, T. Kuboyama & N. Watanabe
Microsatellite mapping of; the Sc; locus conferring screwed spike rachis; to modify; the spatial orientation of; spikelets in; Triticum aestivum L
Genetic Resources and Crop Evolution, 2016年, ［査読有り］

A Stowaway transposon disrupts the InWDR1 gene controlling flower and seed coloration in a medicinal cultivar of the Japanese morning glory
Atsushi Hoshino; Yoshiaki Yoneda; Tsutomu Kuboyama, Floricultural cultivars of the Japanese morning glory (Ipomoea nil) carry transposons of the Tpn1 family as active spontaneous mutagens. Half of the characterized mutations related to floricultural traits were caused by insertion of Tpn1 family elements. In addition, mutations comprising insertions of several bp, presumed to be footprints generated by transposon excisions, were also found. Among these, ca-1 and ca-2 are 7-bp insertions at the same position in the InWDR1 gene, which encodes a multifunctional transcription regulator. InWDR1 enhances anthocyanin pigmentation in blue flowers and red stems, and promotes dark brown seed pigmentation as well as seed-trichome formation. The recessive ca mutants show white flowers and whitish seeds. We characterized here a white flower and whitish seed line that is used as a medicinal herb. The mutant line carries a novel ca allele named ca-3, which is the InWDR1 gene carrying an insertion of a Stowaway-like transposon, InSto1. The ca-3 allele is the first example of a mutation induced by transposons other than those in the Tpn1 family in I. nil. Because InSto1 and the 7-bp putative footprints are inserted at identical positions in InWDR1, ca-3 is likely to be the ancestor of ca-1 and ca-2. According to Japanese historical records on whitish seeds of I. nil, putative ca mutants appeared at the end of the 17th century, at the latest. This is around one hundred years before the appearance of many floricultural mutants. This suggests that ca-3 is one of the oldest mutations, and that its origin is different from that of most floricultural mutations in I. nil., Genetics Society of Japan
Genes and Genetic Systems, 2016年, ［査読有り］

Microsatellite mapping of the gene for sham ramification in spikelets derived from a hexaploid wheat (Triticum spp.) accession 171ACS
Y. Amagai; A. J. Aliyeva; N. Kh. Aminov; P. Martinek; N. Watanabe; T. Kuboyama, Plants with elongated spikelet rachilla (sham ramification) have been found in the progenies of a cross between hexaploid wheat (Triticum aestivum L., 2n = 6x = 42, BBAADD genome) line '171ACS' and durum wheat (T. durum Desf., 2n = 4x = 28, BBAA genome) 'Bereketli-95'. 171ACS was the carrier of the genes for sham ramification and extra glume. Expression of both of these traits was suppressed in hexaploid wheat. The gene for "sham ramification" (shr (171ACS) ) was mapped by genotyping F-2 populations using microsatellite markers. In the F-2 of 171ACS/Bereketli-95, the shr (171ACS) gene was located in chromosome arm 5AL. The shr (171ACS) gene and the gene for extra glume (exg) were completely linked. The shr (171ACS) /exg gene complex was bracketed by the markers Xbarc319 and Xbarc232 on the long arm of chromosome 5A. In F-2 hybrids between #145 and #629, and T. durum 'LD222', the shr (171ACS) gene was completely linked to the exg gene, and the shr (171ACS) /exg gene complex was located in the long arm of chromosome 5A. F-2 hybrids between two tetraploid ramified progenies and T. jakubzineri Udacz. et Shakhm. (2n = 4x = 28, BBAA genome) indicated that shr (171ACS) was allelic to shr1., SPRINGER
GENETIC RESOURCES AND CROP EVOLUTION, 2015年10月, ［査読有り］

Genetic mapping and development of near-isogenic lines for genes governing a liguleless phenotype in tetraploid wheat　　　　　　　　　　　　　　　
Amagai Y; Watanabe N; Kuboyama T, Springer
Euphytica, 2015年, ［査読有り］

Genetic mapping and development of near-isogenic lines with genes conferring mutant phenotypes in Aegilops tauschii and synthetic hexaploid wheat　　　　　　　　　　　　　　　
Amagai Y; Watanabe N; Kuboyama T
Euphytica, 2015年, ［査読有り］

Microsatellite mapping of the mutant gene conferring interrupted development of leaf blade in Triticum aestivum L.　　　　　　　　　　　　　　　
Y. Amagai; N. Watanabe; T. Kuboyama
Genet Resour Crop Evol, 2015年, ［査読有り］

Microsatellite mapping of genes for branched spike and soft glumes in Triticum monococcum L
Amagai Y.; Martinek P.; Watanabe N.; Kuboyama T., The locus responsible for branched spike in a branched spike mutant of Triticum monococcum L. (2n = 2x = 14, A(m)A(m) genome) and soft glume in Triticum sinskajae Filat. et Kurkiev (2n = 2x = 14, A(m)A(m) genome) were mapped by genotyping F-2 populations using microsatellite markers. Phenotypic analysis in the cross T. sinskajae PI 418587/a branched spike mutant KT3-24 confirmed that both characters were under control of a recessive allele at a single locus, and they were linked with 26.6 cM. The branched head in T. monococcum (bh(m)) locus was located on chromosome 2A(m)S and the marker Xgwm122 flanked the bh(m) gene distally. Soft glume locus in T. sinskajae was allelic to the soft glume (sog) locus in mm09, a soft glume mutant of T. monococcum. The sog locus was linked with Xwmc644 distally. In the F-2 hybrids of T. monococcum #252/PI 418587 and T. monococcum KT 3-21/PI 418587, sog was linked with Xgwm71. The gene fg which determines a false glume was also located on chromosome 2A(m)S and the recombination between sog and fg (1.6 cM) was obtained in F-2 hybrid of KT 3-21/PI 418587., SPRINGER
Genet Resour Crop Evol, 2014年, ［査読有り］

Microsatellite mapping of the genes for sham ramification and extra glume in spikelets of tetraploid wheat
Amagai; Y.; Aliyeva A. J.; Aminov N. Kh.; Martinek P.; Watanabe N. &; Kuboyama T., The loci for expression of prolonged spikelet rachilla named 'sham ramification' in three tetraploid wheat accessions, two accessions of Triticum jakubzineri Udacz. et Schachm. (2n = 4x = 28, genome BBAA) 'PI 585014' and 'R-101-03' and one sample of Triticum turgidum L. (2n = 4x = 28, genome BBAA) 'PI 67339', were mapped by genotyping F-2 populations using microsatellite markers. The segregation analysis confirmed that sham ramification was controlled by recessive, epistatic genes, shr1 (sham ramification 1) and shr2 (sham ramification 2). The genotypes of 'sham ramification' were shr1shr1Shr2 Shr2 for T. jakubzineri and Shr1Shr1shr2shr2 for T. turgidum (PI 67339). Normal rachilla was determined by Shr1Shr1Shr2Shr2. The shr1 gene and the gene for extra glume (exg) were completely linked; and, the shr1/exg gene complex was bracketed by the markers Xbarc319 and Xbarc232 on the long arm of chromosome 5A. In the F-2 of PI 67339/LD222 the gene shr2 was bracketed by Xwmc819 and Xwmc794 on the long arm of chromosome 2A., SPRINGER
Genet Resour Crop Evol, 2014年, ［査読有り］

Nicotiana debneyi has a single dominant gene causing hybrid lethality in crosses with N-tabacum
Takahiro Iizuka; Tsutomu Kuboyama; Wataru Marubashi; Masayuki Oda; Takahiro Tezuka, To elucidate the genetic mechanism of hybrid lethality observed in hybrids between cultivated tobacco, Nicotiana tabacum, and wild tobacco species in the section Suaveolentes, genetic analyses were conducted through the triple cross of the hybrids of wild species, including N. benthamiana and N. fragrans, and N. tabacum. N. benthamiana and N. fragrans produced only viable hybrids after crossing with N. tabacum. Subsequently, N. benthamiana and N. fragrans were crossed with N. africana, N. debneyi, and/or N. suaveolens, which produced inviable hybrids after crossing with N. tabacum. Hybrids of the wild species were obtained from four of the six cross combinations. Only when hybrid plants of N. debneyi x N. fragrans, whose hybridity was confirmed by morphological characteristics, random amplified polymorphic DNA analysis, and chromosome observation, were crossed with N. tabacum, triple hybrids were obtained and segregated 1:1 (lethal:viable). Based on these results, a single dominant gene, designated Hybrid Lethality A1 (HLA1), in N. debneyi was found to control hybrid lethality by the interaction with gene(s) on the Q chromosome in N. tabacum. This represents the first report to identify a causal gene for hybrid lethality in the genus Nicotiana., SPRINGER
EUPHYTICA, 2012年07月, ［査読有り］

Microsatellite mapping of genes for semi-dwarfism and branched spike in Triticum durum Desf. var. ramosoobscurum Jakubz. ‘‘Vetvistokoloskaya’’
M. A. Haque; P. Martinek; S. Kobayashi; I. Kita; K. Ohwaku; N. Watanabe; T. Kuboyama, A combination of increased harvest index and increased seed number per plant may improve wheat yield. We found an accession Desf. var. Jakubz. "Vetvistokoloskaya" R-107, whose plant height was short and spike was branched. To characterize R-107, we mapped the genes for semi-dwarfism and branched spike. Semi-dwarf gene (educed eigh R107) from R-107 was allelic to and - on chromosome 4BS. as well as was recessive and insensitive to gibberellic acid. Microsatellite mapping indicated that was linked to (1.2 cM) on chromosome 4BS, and was also linked to marker (19.4 cM) on chromosome 4BS. The locus responsible for branched spike in R-107 was mapped by genotyping three F-2 populations using microsatellite markers. Phenotypic analysis revealed that branched spike was under control of a recessive allele at a single locus. The (ranched ead) locus was located on chromosome 2AS and the marker flanked the gene proximally., SPRINGER
Genet Resour Crop Evol, 2012年06月, ［査読有り］

In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut
Kenta Shirasawa; Padmalatha Koilkonda; Koh Aoki; Hideki Hirakawa; Satoshi Tabata; Manabu Watanabe; Makoto Hasegawa; Hiroyuki Kiyoshima; Shigeru Suzuki; Chikara Kuwata; Yoshiki Naito; Tsutomu Kuboyama; Akihiro Nakaya; Shigemi Sasamoto; Akiko Watanabe; Midori Kato; Kumiko Kawashima; Yoshie Kishida; Mitsuyo Kohara; Atsushi Kurabayashi; Chika Takahashi; Hisano Tsuruoka; Tsuyuko Wada; Sachiko Isobe, Background: Peanut (Arachis hypogaea) is an autogamous allotetraploid legume (2n = 4x = 40) that is widely cultivated as a food and oil crop. More than 6,000 DNA markers have been developed in Arachis spp., but high-density linkage maps useful for genetics, genomics, and breeding have not been constructed due to extremely low genetic diversity. Polymorphic marker loci are useful for the construction of such high-density linkage maps. The present study used in silico analysis to develop simple sequence repeat-based and transposon-based markers.
Results: The use of in silico analysis increased the efficiency of polymorphic marker development by more than 3-fold. In total, 926 (34.2%) of 2,702 markers showed polymorphisms between parental lines of the mapping population. Linkage analysis of the 926 markers along with 253 polymorphic markers selected from 4,449 published markers generated 21 linkage groups covering 2,166.4 cM with 1,114 loci. Based on the map thus produced, 23 quantitative trait loci (QTLs) for 15 agronomical traits were detected. Another linkage map with 326 loci was also constructed and revealed a relationship between the genotypes of the FAD2 genes and the ratio of oleic/linoleic acid in peanut seed.
Conclusions: In silico analysis of polymorphisms increased the efficiency of polymorphic marker development, and contributed to the construction of high-density linkage maps in cultivated peanut. The resultant maps were applicable to QTL analysis. Marker subsets and linkage maps developed in this study should be useful for genetics, genomics, and breeding in Arachis. The data are available at the Kazusa DNA Marker Database (http://marker.kazusa.or.jp)., BIOMED CENTRAL LTD
BMC PLANT BIOLOGY, 2012年06月, ［査読有り］

Temperature-dependent enhancement of pollen tube growth observed in interspecific crosses between wild Cucumis spp. and melon (C. melo L)
Yuichi Matsumoto; Makoto Miyagi; Nobuyoshi Watanabe; Tsutomu Kuboyama, Wild Cucumis spp., which have resistance to various diseases, have the potential to be used for melon (C. melo L) breeding. Nevertheless, few interspecific hybrids between melon and wild Cucumis spp. have been obtained because of strong reproductive barriers such as pollen-pistil incongruity and hybrid seed abortion. For this study, we examined the temperature conditions enhancing pollen tube growth and fruit set in interspecific crosses between melon and wild Cucumis spp. Before temperature-conditioned pollination, four wild Cucumis spp. were pollinated with the melon line 'MR-1' and two lines of C. anguria, PI 147065 and PI 320052, and a line of C. metuliferus, PI 526242, were selected for later experiments. These three lines were crossed reciprocally with 'MR-1' under controlled temperatures of 24-34 degrees C at 2 degrees C intervals. When 'MR-1' pistils were pollinated with three lines of wild Cucumis spp., pollen tube growth was arrested after germination at all temperatures. However, in the pistil of wild Cucumis spp., pollen tube growth of 'MR-1' was significantly greater at 32 degrees C and at 34 degrees C than that under 30 degrees C. Furthermore, pollen tube length in pistils of PI 320052 and PI 526242 at 32 degrees C was comparable to that of 'MR-1' self-pollination at 28 degrees C. Fruit set frequency was also improved at 32 degrees C. These results demonstrated that the pollen tube inhibition of Cucumis spp. interspecific crosses might be partially or fully recoverable in higher-temperature conditions. Moreover, 32 degrees C is apparently the threshold temperature for promoting pollen tube growth in these cross combinations. (C) 2012 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
SCIENTIA HORTICULTURAE, 2012年05月, ［査読有り］

Cross-species transferability of 86 cucumber (Cucumis sativus L.) microsatellite markers to gherkin (C. anguria L.)
Yuichi Matsumoto; Nobuyoshi Watanabe; Tsutomu Kuboyama, Gherkin (Cucumis anguria L.), also known as West Indian gherkin, burr gherkin, and maxixe, is mainly cultivated and consumed in Brazil and the United States. Marker-assisted selection (MAS) would be a highly desirable tool for breeding of gherkin as gherkin cultivation generally requires considerable time, labor, and space. However, DNA markers available for gherkin have rarely been reported, although many microsatellite linkage maps have been constructed for cucumber and whole genomic sequences have been reported. In this study, we attempted to transfer 86 microsatellite markers derived from cucumber to gherkin and assess amplification and polymorphism between two gherkin accessions to enable the use of the microsatellite markers in gherkin. In PI 320052 and PI 364475, 58 (67.4%) and 55 (64.0%) microsatellite markers were successfully amplified, respectively. Of the amplified markers, the polymorphism number between the two accessions was 40. Thus, an adequate number of cucumber microsatellite markers can be transferred to gherkin. Current polymorphic microsatellite markers could then be made available for the study of landmarks in linkages, genomic structures, evolutionary ecology, and MAS in gherkin and Cucurbitaceae. (c) 2012 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
SCIENTIA HORTICULTURAE, 2012年03月, ［査読有り］

Genetic mapping of the gene for brittle rachis in a Triticum aestivum—Aegilops triuncialis introgression line
K. Yoshiya; N. Watanabe; T. Kuboyama; I. F. Lapochkina, Aegilops triuncialis L. (2n = 4x = 28, genome formula UUCC) has been used to obtain one of the "Arsenal collection", the distant hybridization of Triticum aestivum L. The introgression line 124/00(i), which was derived from the cross of T. aestivum 'Rodina' with gamma-irradiated pollen of an accession of Ae. triuncialis. However, undesirable genes can be incorporated into the cultivated varieties from Ae. triuncialis. The spikes of Ae. triuncialis disarticulate from stems at a single node at the bottom of the spikes. It was defined as a synaptospermic diaspore. However, the spike of 124/00(i) unexpectedly disarticulate below each spikelet showing wedge type of disarticulation. The brittle rachis gene Br (124) (<Emphasis Type="ItalicUnderline">B rittle <Emphasis Type="ItalicUnderline">r achis of <Emphasis Type="ItalicUnderline">124 /00 (i) ) of 124/00(i) was allelic to Br2 located on the short arm of chromosome 3A. From the microsatellite mapping, the Br (124) gene was bracketed by Xbarc19 (20.3 cM) and Xgwm779.1(14.3 cM) on the short arm of chromosome 3A. The present study suggested that the dominant mutation from synaptospermic diaspore to wedge type disarticulation occurred in the gamete of Ae. triuncialis when Ae. triuncialis pollen was treated with gamma-irradiation., SPRINGER
Genet Resour Crop Evol, 2012年01月, ［査読有り］

Development of EST-SSR Markers of Ipomoea nil
Ly Tong; Hiroyuki Fukuoka; Asami Otaka; Atsushi Hoshino; Shigeru Iida; Eiji Nitasaka; Nobuyoshi Watanabe and Tsutomu Kuboyama, 責任著者, Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives., JAPANESE SOC BREEDING
Breeding Science, 2012年, ［査読有り］

Cytological and genetic mapping of the gene for four-awned phenotype in Triticum carthlicum Nevski
M. A. Haque; A. Takayama; N. Watanabe; T. Kuboyama, A striking feature of Triticum carthlicum Nevski (2n = 4x = 28, AABB genome) is an awn-like appendage on the glume, so all the spikelets appear four-awned. From the word "tetraaristatus" (four-awned), the locus was named "t" to distinguish it from the b (1) locus on chromosome 5A and b2 locus on chromosome 6B for awn formation. The aims of present study was to determine the chromosomal location of the t locus in tetraploid wheat T. carthlicum, and (2) to assess linkage relationships of t, Rht12 using microsatellite markers in T. carthlicum. Combined cytological evidence and microsatellite mapping showed that t was located on the chromosome 5AL. The alignment of the gene was Xgwm291-(6.4 cM)-t-(6.7 cM)-Xgwm410 in F(2) of T. carthlicum #521/LD222, and Xhbg219-(12.4 cM)-t-(5.1 cM)-Xgwm410-(6.2 cM) -Rht12 in F(2) of ANW 16C/#521 on the 5AL. The t locus was different from b1 locus, because it was known that the semi-dwarf Rht12 gene was completely linked with b1 gene. We discussed that the four-awned phenotype of T. carthlicum was introduced from Triticum aestivum L. ssp. carthlicoides., SPRINGER
GENETIC RESOURCES AND CROP EVOLUTION, 2011年10月, ［査読有り］

Genetic Mapping of Gibberellic Acid-sensitive Genes for Semi-dwarfism in Durum Wheat
M. A. Haque; P. Martinek; N. Watanabe; T. Kuboyama, Semi-dwarf varieties in wheat associated with gibberellic acid (GA(3))-insensitive height reducing genes have led to significant increases in yield but often fall below this potential because of poor seedling emergence after deep showing. Alternative semi-dwarf genes may have the potential to reduce plant height without compromising early plant growth. In durum wheat, bulk segregant analysis was used to screen microsatellite markers linked with the GA(3)-sensitive genes Rht14 in cv. Castelporziano, Rht16 in cv. Edmore M1 and Rht18 in cv. Icaro. Molecular marker Xbarc3-6A for Rht14, Rht16 and Rht18 showed significant polymorphic differences among DNA bulks for height classes. The genes Rht14, Rht16 and Rht18 were linked with Xbarc3 (11.7-28.0 cM) on the short arm of chromosome 6A and they appear to be allelic. Semi-dwarf genes on chromosome 6AS may potentially be used in breeding for improved establishment., AKADEMIAI KIADO RT
CEREAL RESEARCH COMMUNICATIONS, 2011年06月, ［査読有り］

Chromosomal Location of HWA1 and HWA2, Complementary Hybrid Weakness Genes in Rice
Katsuyuki Ichitani; Satoru Taura; Takahiro Tezuka; Yuuya Okiyama; Tsutomu Kuboyama, Hybrid weakness phenomena in rice reportedly have two causes: those of HWC1 and HWC2 genes and those of HWA1 and HWA2 genes. No detailed study of the latter has been reported. For this study, we first produced crosses among cultivars carrying the weakness-causing allele on the HWA1 and HWA2 loci to confirm the phenotype of the hybrid weakness and the genotypes of the cultivars on the two loci, as reported earlier. We then confirmed that these cultivars belong to Indica. Subsequent linkage analysis of HWA1 and HWA2 genes conducted using DNA markers revealed that both genes are located in the 1,637-kb region, surrounded by the same DNA markers on the long arm of chromosome 11. The possibility of allelic interaction inducing hybrid weakness is discussed., SPRINGER
RICE, 2011年06月, ［査読有り］

Comparative genetic mapping of homoeologous genes for the chlorina phenotype in the genus Triticum
K. Kosuge; N. Watanabe; T. Kuboyama, Triticum monococcum L. (2n = 2x = 14, A(m)A(m) genome) is one of the most ancient of the domesticated crops in the Middle East, but it is not the ancestor of the A genome of durum wheat (T. durum Desf. 2n = 4x = 28, genomes BBAA) and bread wheat (T. aestivum L., 2n = 6x = 42, genomes BBAADD). It has been suggested that some differentiation has occurred between the A(m) and A genomes. The chlorina mutants at the cn-A1 locus located on chromosome 7AL have been described in T. aestivum L. and T. durum, and a chlorina mutant has been found in T. monococcum. The aims of our study were to establish linkage maps for chlorina mutant genes on chromosome 7A of T. aestivum and T. durum and chromosome 7A(m) of T. monococcum and to discuss the differentiation that has occurred between the A and A(m) genomes. The chlorina mutant gene was found to be linked with Xhbg234 (8.0 cM) and Xgwm282 (4.3 cM) in F-2 plants of T. aestivum ANK-32A/T. petropavlovskyi k54716, and with Xbarc192 (19.5 cM) and Xgwm282 (12.0 cM) in F-2 plants of T. durum ANW5A-7A/T. carthlicum #521. Both the hexaploid and tetraploid wheats contained a common marker, Xgwm282. In F-2 lines of T. monococcum KT 3-21/T. sinskajae, the cn-A1 locus was bracketed by Xgwm748 (25.7 cM) and Xhbg412 (30.8 cM) on chromosome 7A(m)L. The distal markers, Xhbg412, Xgwm282, and Xgwm332, were tightly linked in T. aestivum and T. durum. The common marker Xhbg412 indicated that the chlorina mutant genes are located on chromosome 7AL and that they are homoeologous mutations., SPRINGER
EUPHYTICA, 2011年05月, ［査読有り］

Genetic mapping of the genes for non-glaucous phenotypes in tetraploid wheat
K. Yoshiya; N. Watanabe; T. Kuboyama, Glaucousness is a visual trait related to the colour of the photosynthetic surface and hence it can be easily selected. It is associated both with the deposition and orientation of wax platelets on the cuticle of the photosynthetic surface. It is known that the glaucous leaf character is determined by the W1 gene, and Iw1 and Iw1 (DIC) genes from Triticum dicoccoides, which act as an epistatic inhibitor to a glaucousness. The aim of the present study was to map W1, Iw1 and Iw1 (DIC) from T. dicoccoides in the short arm of chromosome 2B of tetraploid wheat. Segregation of F(2) populations of three hybrids indicated that the marker Xgwm455 is linked with and distal to Iw1 (16.7 cM), two markers are tightly linked with W1, and Iw1 (DIC) is linked with Xgwm614 and Xwmc661 distally located on chromosome 2BS. From three derived maps, it is suggested that Iw1 (DIC) locus is different from W1. We discuss the difference between Vir and Iw1 (DIC) from T. dicoccoides., SPRINGER
EUPHYTICA, 2011年01月, ［査読有り］

Response of Wild Cucumis Species to Inoculation with Fusarium oxysporum f. sp. melonis Race 1,2y
Yuichi Matsumoto; Takashi Ogawara; Makoto Miyagi; Nobuyoshi Watanabe and Tsutomu Kuboyama, Fusarium wilt of melon (Cucumis melo L.), caused by Fusarium oxysporum f. sp. melonis, is regarded as a severe disease worldwide. Many resources that are resistant to races 0, 1, and 2 have been reported; nevertheless, no resource of high resistance to either race 1,2w or 1,2y has been reported, although partial resistance has been found. Wild Cucumis species have been reported as genetic resources which have resistance to some diseases and pests of melon. This study identified novel sources of high resistance to race 1,2y in wild Cucumis species. In all, we tested 76 accessions belonging to 11 wild species of the genus Cucumis. After artificial inoculation, the disease severity in each plant was evaluated using a 0-3 disease severity scale (0=no symptoms, 1=beginning of yellowing symptom on leaves, 2=leaves strongly affected, 3=plant death). The disease index (DI) was calculated as DI=summation of (disease severity x number of plants in that disease severity) x 100/(3 x total number of plants). Accessions showing high resistance (DI=0) were 34 among six species: C. africanus, C. anguria, C. metuliferus, C. prophetarum, C. subsericeus, and C. zeyheri. By contrast, most accessions in C. dipsaceus, C. meeusei, C. pustulatus, and C. sagittatus exhibited high susceptibility (DI=80-100). Among these four species, no accession showed high resistance. This is the first report of genetic resources having high resistance to race 1,2y. To introduce this high resistance of race 1,2y to melon, we should investigate methods to overcome reproductive barriers to interspecific crosses between melon and wild Cucumis species., JAPAN SOC HORTICULTURAL SCI
J. Japan. Soc. Hort. Sci., 2011年, ［査読有り］

Seven of eight species in Nicotiana section Suaveolentes have common factors leading to hybrid lethality in crosses with Nicotiana tabacum
Takahiro Tezuka; Tsutomu Kuboyama; Toshiaki Matsuda; Wataru Marubashi, Background and Aims Reproductive isolation is a mechanism that separates species, and is classified into two types: prezygotic and postzygotic. Inviability of hybrids, or hybrid lethality, is a type of postzygotic isolation and is observed in some plant species, including Nicotiana species. Previous work has shown that the Q chromosome, which belongs to the S subgenome of N. tabacum, encodes one or more genes leading to hybrid lethality in some crosses.
Methods Interspecific crosses of eight wild species were conducted in section Suaveolentes ( which consists of species restricted to Australasia and Africa) with the cultivated species Nicotiana tabacum. Hybrid seedlings were cultivated at 28, 34 or 36 degrees C, and PCR and chromosome analysis were performed.
Results and Conclusions Seven of eight wild species produced inviable hybrids after crossing. Hybrid lethality, which was observed in all crosses at 28 degrees C, was Type II lethality, with the characteristic symptoms of browning of hypocotyl and roots; lethality was suppressed at elevated temperatures ( 34 or 36 degrees C). Furthermore, one or more genes on the Q chromosome of N. tabacum were absolutely responsible for hybrid lethality, suggesting that many species of section Suaveolentes share the same factor that triggers hybrid lethality by interaction with the genes on the Q chromosome. Exceptionally, only one wild species, N. fragrans, produced 100% viable hybrids after crossing with N. tabacum, suggesting that N. fragrans has no factor triggering hybrid lethality., OXFORD UNIV PRESS
ANNALS OF BOTANY, 2010年08月, ［査読有り］

Recombination around the P locus for long glume phenotype in experimental introgression lines of Triticum aestivum – Triticum polonicum
K. Kosuge; N. Watanabe; T. Kuboyama, A set of experimental introgression lines of Triticum aestivum L. cv. Novosibirskaya 67 (N67)-Triticum polonicum L. line IC 12196 was developed using a small-scale bulk breeding method. The linkage map in chromosome 7A was constructed using F(2) hybrids of N67/IC12196 and 34 microsatellite markers. The P gene was flanked by the centromeric markers, Xgwm890 (18.6 cM) and Xbarc108 (20.0 cM) on the long arm of chromosome 7A. Among 124 introgression lines, 118 lines were hexaploid (2n = 6x = 42), and 6 were tetraploid (2n = 4x = 28). Among hexaploid accessions, 68 were long-glumed, whereas 50 were normal-glumed. Thirty-four polymorphic microsatellite markers were scored for either the N67 alleles or IC 12196 alleles in 124 introgression lines derived from N67/IC 12196. The UPGMA dendrogram showed five clusters; Cluster 1 mainly contained hexaploid introgression lines with long glumes. Although the alleles around the P locus were recombined with IC1296 alleles, the distal end of the chromosome contained N67 alleles. Cluster 2 mainly contained normal glumed, hexaploid introgression lines. These predominantly had the N67 alleles on the long arm of chromosome 7A and the short arm proximal to the centromere. Cluster 3 contained long-glumed, hexaploid wheat lines with relatively high level of recombination. Cluster 4 contained non-parental alleles. Cluster 5 contained the group of tetraploid wheat lines. These tetraploid lines have IC12196 alleles on both arms of chromosome 7A. The frequency spectrum of parental alleles and chromosomal blocks among introgression lines suggested that T. aestivum - T. polonicum hybridization can rapidly give rise to a new landrace due to selective introgression of the P gene., SPRINGER
Genetic Resources and Crop Evolution, 2010年, ［査読有り］

Fine Mapping of HWC2, a Complementary Hybrid Weakness Gene, and Haplotype Analysis Around the Locus in Rice
Tsutomu Kuboyama; Toshiya Saito; Takashi Matsumoto; Jianzhong Wu; Hiroyuki Kanamori; Satoru Taura; Muneharu Sato; Wataru Marubashi; Katsuyuki Ichitani, Hybrid weakness is a reproductive barrier. In rice, the hybrid weakness caused by two complementary genes-HWC1 and HWC2-has been surveyed extensively. However, their gene products and the molecular mechanism that causes hybrid weakness have remained unknown. We first performed fine mapping of HWC2, narrowing down the area of interest to 19 kb. We thereby identified five candidate genes. Second, we performed haplotype analysis around the HWC2 locus of 33 cultivars. With 15 DNA markers examined, all the 13 Hwc2-1 carriers share the same haplotype for consecutive 14 DNA markers. As for hwc2-2 carriers, five out of 20 have the haplotypes relatively similar to those of Hwc2-1 carriers. However, the other haplotypes differ remarkably from them. These results are useful to identify the HWC2 gene and to study rice varietal differentiation., SPRINGER
RICE, 2009年09月, ［査読有り］

Genetic diversity and relationship analysis of peanut germplasm using SSR markers
Yoshiki Naito; Shigeru Suzuki; Yoshiharu Iwata and Tsutomu Kuboyama, Peanut (Arachis hypogaea L.), an important source of oil and protein, is native to South America. Most peanuts grown in Japan are large-grain cultivars that are eaten mainly as a snack. The peanut germplasm was introduced from abroad over a century ago and has been used for breeding. Although many peanut varieties have been developed, the relationship between Japanese varieties and peanut phylogeny remains unclear; therefore, this study assessed the diversity and genetic relationships within the peanut germplasms in Japan using allelic variation in a selected set of 13 SSR markers. We analyzed 201 accessions of A. hypogaea and 13 accessions of Arachis wild species: 13 primer pairs amplified 108 polymorphic alleles in A. hypogaea. The detected alleles were 3-15 at each of the 13 markers, with an average of 8.3 per marker. The phenogram based on the SSR genotypes was obtained. A. hypogaea and A. monticola made a separate group from diploid species; they were classified into 150 genotypes. A. hypogaea and A. monticola were divided further into two groups; the first group consisted mainly of spp. fastigiata accessions; the second group consisted mainly of spp. hypogaea accessions and tetraploid wild peanut A. monticola., JAPANESE SOC BREEDING
Breeding Science, 2008年, ［査読有り］

Genetic mapping of a mutant gene producing three pistils per floret in common wheat
Zheng-Song Peng; Petr Martinek; Kazumasa Kosuge; Tsutomu Kuboyama and Nobuyoshi Watanabe, A common wheat (Trilicum aestivum L.) mutation that produces 3 pistils (TP) per floret may result in formation of Lip to 3 kernels per floret. The TP trait may be important for increasing the number of grains per spike and for improving the yield potential through breeding. This trait is determined by the dominant Pis1 gene. Genetic mapping of Pis1 involved 234 microsatellite markers and bulk segregant analysis of a cross of the TP line with Novosibirskaya 67. The Pis1 gene is located on chromosome 2DL, between markers Xgwm539 and Xgwm349. This result does not agree with a previously published localization of the Pis1 gene on chromosome 5B. The possible importance of TP wheat as an alternative genetic resource is discussed., SPRINGER HEIDELBERG
Journal of Applied Genetics, 2008年, ［査読有り］

Fine mapping and allelic dosage effect of Hwc1, a complementary hybrid weakness gene in rice
Katsuyuki Ichitani; Keita Namigoshi; Muneharu Sato; Satoru Taura; Misato Aoki; Yuichi Matsumoto; Toshiya Saitou; Wataru Marubashi; Tsutomu Kuboyama, Hybrid weakness is a reproductive barrier that is found in many plant species. In rice, the hybrid weakness caused by two complementary genes, Hwc1 and Hwc2, has been surveyed intensively. However, their gene products and the molecular mechanism that causes hybrid weakness have remained unknown. We performed linkage analyses of Hwc1, narrowed down the area of interest to 60 kb, and identified eight candidate genes. In the F-2 population, in which both Hwc1 and Hwc2 genes were segregated, plants were separable into four classes according to their respective phenotypes: severe type, semi-severe type, F-1 type, and normal type. Severe type plants show such severe symptoms that they could produce only tiny shoot-like structures; they were unable to generate roots. Genetic analyses using closely linked DNA markers of the two genes showed that the symptoms of the F-2 plants were explainable by the genotypes of Hwc1 and Hwc2. Weakness was observed in plants that have both Hwc1 and Hwc2. In Hwc1 homozygote, the symptoms worsened and severe type or semi-severe type plants appeared. Consequently, Hwc1 should have a gene dosage effect and be a semi-dominant gene. The dosage effect of Hwc2 was recognizable, but it was not so severe as that in Hwc1. These results are useful to elucidate the mechanism that causes the hybrid weakness phenomenon and the role of each causal gene in hybrid weakness., SPRINGER
THEORETICAL AND APPLIED GENETICS, 2007年05月, ［査読有り］

Identification and characterization of genes involved in hybrid lethality in hybrid tobacco cells(Nicotiana suaveolens x N. tabacum) using suppression subtractive hybridization
Yu Masuda; Tetsuya Yamada; Tsutomu Kuboyama and Wataru Marubashi, Hybrid lethality is an important problem for cross-breeding; however, its molecular mechanism is not clear. The purpose of the present study was to identify the genes expressed during hybrid lethality in the hybrid cells (Nicotiana suaveolens x N. tabacum). In order to identify these genes, we employed suppression subtractive hybridization (SSH) between RNA isolated from cells expressing lethality (lethal hybrid line; LH line) and cells overcoming lethality fortuitously (a surviving hybrid line; SH line). Four populations of cDNA were created from the time points corresponding to before and during induction, and at and after the point of no return (PNR) during the process of programmed cell death (PCD) that occurs during hybrid lethality. By SSH and following dot-blot macroarray analysis, 99 genes out of 138 isolated clones were identified as hybrid lethality-related (HLR) genes. Quantitative real-time PCR analysis data indicated that ten clones were expressed specifically in LH line cells. The HLR genes in these clones show homology to genes involved in disease resistance, ethylene-induced reactions, phosphorylation, ubiquitination, jasmonic acid-related reactions, calcium signaling and self-incompatibility. These data suggested that at least some parts of the mechanism of hybrid lethality are shared with those of the putative functions of the HLR gene-related pathways., SPRINGER
Plant Cell Reports, 2007年, ［査読有り］

Cytological and microsatellite mapping of mutant genes for spherical grain and compact spikes in durum wheat　　　　　　　　　　　　　　　
K. Kosuge; AE N. Watanabe; AE T; Kuboyama AE; V. M. Melnik; AE V. I; Yanchenko AE; M. A. Rosova AE N; P. Goncharov
Euphytica, 2007年, ［査読有り］

Possible involvement of genes on the Q chromosome of Nicotiana tabacum in expression of hybrid lethality and programmed cell death during interspecific hybridization to Nicotiana debneyi
Tezuka T; Kuboyama T; Matsuda Yu and Marubashi W, Hybrid seedlings from the cross between Nicotiana tabacum, an allotetraploid composed of S and T subgenomes, and N. debneyi die at the cotyledonary stage. This lethality involves programmed cell death (PCD). We carried out reciprocal crosses between the two progenitors of N. tabacum, N. sylvestris and N. tomentosiformis, and N. debneyi to reveal whether only the S subgenome in N. tabacum is related to hybrid lethality. Hybrid seedlings from reciprocal crosses between N. sylvestris and N. debneyi showed lethal characteristics identical to those from the cross between N. tabacum and N. debneyi. Conversely, hybrid seedlings from reciprocal crosses between N. tomentosiformis and N. debneyi were viable. Furthermore, hallmarks of PCD were observed in hybrid seedlings from the cross N. debneyi x N. sylvestris, but not in hybrid seedlings from the cross N. debneyi x N. tomentosiformis. We also carried out crosses between monosomic lines of N. tabacum lacking the Q chromosome and N. debneyi. Using Q-chromosome-specific DNA markers, hybrid seedlings were divided into two groups, hybrids possessing the Q chromosome and hybrids lacking the Q chromosome. Hybrids possessing the Q chromosome died with characteristics of PCD. However, hybrids lacking the Q chromosome were viable and PCD did not occur. From these results, we concluded that the Q chromosome belonging to the S subgenome of N. tabacum encodes gene(s) leading to hybrid lethality in the cross N. tabacum x N. debneyi., SPRINGER
Planta, 2007年, ［査読有り］

Developmental observation and high temperature rescue from hybrid weakness in a cross between Japanese rice cultivars and Peruvian rice cultivar ‘Jamaica’.
Toshiya Saito; Katsuyuki Ichitani; Tsukasa Suzuki; Wataru Marubashi; Tsutomu Kuboyama, 責任著者, The weak growth occurring in hybrids derived from crosses between two normal strains is referred to as hybrid weakness. F, hybrid weakness (F1HW) in a cross between a Peruviari rice cultivar 'Jamaica' and Japanese rice cultivars has been reported and was considered to be controlled by a set of complementary genes, Hwc1 and Hwc2. We observed the development of F, plants between Nipponbare and Jamaica (NJF(1)s). NJF(1)s were characterized by short roots, rolled leaves and a short stature. Although the NJF(1) embryos were normal, the growth of the primary roots was arrested and in most cases, they could not emerge from seeds of the hybrids. Histological observation of the root apical meristem (RAM) at 10 days after sowing revealed that the zone of cell division in the NJF(1) plants was lost, and that the cells were not short enough to explain the reduced root length of the NJF(1) plants. Thus, it was suggested that the shorter roots the NJF(1) plants might be due to reduced cell division. Although no distinct morphological abnormalities in the shoot apical meristem were observed, leaf primordium production was delayed in the NJF(1) plants. Inhibition of root elongation in this type of hybrid weakness was alleviated under high temperature (34 degrees C) conditions in diverse genetic backgrounds. The threshold temperature required for the recovery of root growth from hybrid weakness seemed to range from 29 to 30 degrees C. Among 85 NJF(1) plants that germinated and grew at 34 degrees C for 10 days, and then outdoors, 18 plants eventually flowered. These plants were dwarf and their flowering time was delayed compared with that of their parents. In spite of their abnormal development, they were fertile. Thus, it is suggested that high temperature treatment was also effective to overcome hybrid weakness and to obtain next generation in this cross combination., JAPANESE SOC BREEDING
Breeding Science, 2007年, ［査読有り］

The Q chromosome of Nicotiana tabacum controls hybrid lethality in interspecific hybrids between N. tabacum and N. debneyi　　　　　　　　　　　　　　　
Takahiro Tezuka; Tsutomu Kuboyama; Toshiaki Matsuda; Wataru Marubashi
Proceedings of the 10th International Congress of SABRAO, 2005年03月

Two different thymidylate kinase gene homologues, including one that has catalytic activity, are encoded in the onion yellows phytoplasma genome.
Miyata S; Oshima K; Kakizawa S; Nishigawa H; Jung HY; Kuboyama T; Ugaki M; Namba S., Thymidylate kinase (TMK) catalyses the phosphorylation of dTMP to form dTDP in both the de novo and salvage pathways of dTTP synthesis in both prokaryotes and eukaryotes. Two homologues of bacterial thymidylate kinase genes were identified in a genomic library of the onion yellows (OY) phytoplasma, a plant pathogen that inhabits both plant phloem and the organs of insects. Southern blotting analysis suggested that the OY genome contained one copy of the tmk-b gene and multiple copies of the tmk-a gene. Sequencing of PCR products generated by amplification of tmk-a enabled identification of three other copies of tmk-a, although the ORF in each of these was interrupted by point mutations. The proteins, TMK-a and TMK-b, encoded by the two intact genes contained conserved motifs for catalytic activity. Both proteins were overexpressed as fusion proteins with a polyhistidine tag in Escherichia coli and purified, and TMK-b was shown to have thymidylate kinase activity. This is believed to be the first report of the catalytic activity of a phytoplasmal protein, and the OY phytoplasma is the first bacterial species to be found to have two intact homologues of tmk in its genome., SOC GENERAL MICROBIOLOGY
Microbiology, 2003年, ［査読有り］

Evidence of intermolecular recombination between extrachromosomal DNAs in phytoplasma: a trigger for the biological diversity of phytoplasma?
Nishigawa H; Oshima K; Kakizawa S; Jung HY; Kuboyama T; Miyata S; Ugaki M; Namba S
Microbiology (Reading, England), 2002年05月, ［査読有り］

The gene arrangement and sequence of str operon of phytoplasma resemble those of Bacillus more than those of Mycoplasma
Shin-ichi Miyata; Ken-ichiro Furuki; Toshimi Sawayanagi; Kenro Oshima; Tsutomu Kuboyama; Tsuneo Tsuchizaki; Masashi Ugaki; Shigetou Namba, The complete region of a putative streptomycin operon (str operon) of onion yellows (OY) phytoplasma, a phytopathogenic mollicute, was isolated and sequenced. This operon contains four genes, rps12, rps7, fus, and tuf, encoding ribosomal proteins S12 and S7, elongation factor (EF) -G, and EF-Tu, respectively. These four genes constitute the str operon in non-mollicute bacteria, such as Escherichia coli and Bacillus subtilis. In two species of mollicute Mycoplasma, the tuf gene was reported not to be included in this operon, but was located apart, indicating that the gene arrangement of this operon in phytoplasmas resembles that of B. subtilis more than that of Mycoplasma spp. In addition, the deduced amino acid sequence of EF-G of phytoplasmas also resembles that of B. subtilis more than that of Mycoplasma spp. These results suggest that analyses of the gene organization and sequence of the phytoplasma genome will provide valuable insights into evolutionary relationships among the culturable mollicutes, phytoplasmas and other Gram-positive bacteria., Phytopathological Society of Japan
J. Gen. Plant Pathol., 2002年, ［査読有り］

A plasmid from a non-insect-transmissible line of a phytoplasma lacks two open reading frames that exist in the plasmid from the wild-type line
Hisashi Nishigawa; Kenro Oshima; Shigeyuki Kakizawa; Hee-Young Jung; Tsutomu Kuboyama; Shin-ichi Miyata; Masashi Ugaki; Shigetou Namba, Two novel rolling circle replication (RCR) plasmids, pOYM (3932 nt) and pOYNIM (3062 nt), were isolated from a mildly pathogenic variant line (OY-M) and a mildly pathogenic plus non-insect-transmissible line (OY-NIM), respectively, of onion yellows (OY) phytoplasma, a plant and insect endocellular mollicute. OY-M was isolated from an original wild-type line (OY-W) after regular maintenance using alternate plant/insect infections, while OY-NIM was further isolated from OY-M after maintenance by plant grafting without insect vectors. The RCR-initiator proteins (Rep) of both plasmids, which have a characteristic structure with both plasmid- and virus-like domains, were highly homologous to that of a previously described OY-W plasmid, pOYW (3933 nt), and were expressed in OY-M- and OY-NIM-infected plants, indicating that this replicon is stably maintained in the phytoplasma. Interestingly, pOYNIM lacked two ORFs that exist in both pOYW and pOYM, which encode a single-stranded DNA binding protein (SSB) and an uncharacterized putative membrane protein, indicating that these two proteins are not necessary for the phytoplasma to live in plant cells. These are the first candidates as phytoplasma proteins possibly related to host specificity. (C) 2002 Elsevier Science B.V. All rights reserved., ELSEVIER SCIENCE BV
Gene, 2002年, ［査読有り］

Isolation and characterization of derivative lines of the onion yellows phytoplasma that do not cause stunting or phloem hyperplasia
Oshima K; Shiomi T; Kuboyama T; Sawayanagi T; Nishigawa H; Kakizawa S; Miyata S; Ugaki M; Namba S
Phytopathology, 2001年, ［査読有り］

A subtilisin-like serine protease specifically expressed in reproductive organs in rice
Kaoru T. Yoshida・Tsutomu Kuboyama, To identify genes specifically expressed in flowering pistils and that are related to reproductive phenomena, simplified differential display was performed with cDNA obtained from pistils and ovaries at several stages. One clone preferentially expressed in pistils at flowering and 1 day before flowering was identified as a rice subtilisin-like serine protease (RSP1). Sequence comparisons revealed that RSP1 has several characteristics in common with preproproteins. RT-PCR, northern blot, and in situ hybridization analysis revealed that RSP1 mRNA accumulates in pistils and in the filaments of stamens, whereas mRNA was undetectable in tissue from leaves, roots, panicles, and embryos. The mRNA levels in pistils increased slightly at flowering and decreased afterwards. Possible roles of the subtilisin-like serine protease in plant reproduction are discussed., SPRINGER-VERLAG
Sexual Plant Reproduciton, 2001年, ［査読有り］

Antiserum against a stigma-exudate protein of tobacco, SE32, which was identical with PPAL , a beta-expansin-like protein specific to stigma, cross-reacted with another stigma-exudate protein, SE35
Tsutomu Kuboyama; Kaoru T. Yoshida and Genkichi Takeda, 筆頭著者, In the first step of pollination in tobacco, pollen grains attach to the stigma exudate, which we previously found to contain two major stigma-exudate proteins, SE32 (32 kDa) and SE35 (35 kDa) (Kuboyama et al 1997). In the present study, we determined each N-terminal amino acid sequence. The N-terminal amino acid sequence of SE32 was identical to that of PPAL, a stigma specific protein which was similar to grass pollen allergens and beta -expansin. However, no sequences identical to the N-terminal amino acid sequence of SE35 were found in the protein databases. An antiserum raised against SE32 was found to react with both SE32 and SE35 in SDS-gel blot analysis. SE32 was detected only in the stigma, while SE35 in both the stigma and the style. These two proteins were not detected in other floral or vegetative organs. Immunohistochemical analysis showed that the antigens of the anti-SE32 antiserum were localized in the extracellular space of transmitting tissue. Accumulation of SE32 started in the completely elongated bud and increased with flower maturation. However, accumulation of SE35 was already detected in the young bud. The antigenicity of SE35 to anti-SE32 antiserum showed structural similarity between SE32 and SE35, and SE35 might belong to the same protein family as SE32. SE32 and SE35 might soften transmitting tissue of the stigma and style, and they might play a role in the elongation of the transmitting-tissue cells or the penetration of the pollen tube into the tissue., JAPANESE SOC BREEDING
Breeding Science, 2001年, ［査読有り］

In planta expression of a protein encoded by the extrachromosomal DNA of phytoplasma and related to geminivirus replication proteins
Hisashi Nishigawa; Shin-ichi Miyata; Kenro Oshima; Toshimi Sawayanagi; Akihiro Komoto; Tsutomu Kuboyama; Izumi Matsuda; Tsuneo Tsuchizaki and Shigetou Namba, A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M. a mild symptom line derived from OY-W, We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7.0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, Suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes., SOC GENERAL MICROBIOLOGY
Microbiology, 2001年, ［査読有り］

A plasmid of phytoplasma encodes a unique replication protein having both plasmid- and virus-like domains: clue to viral ancestry or result of virus/plasmid recombination?
Kenro Oshima; Shigeyuki Kakizawa; Hisashi Nishigawa; Tsutomu Kuboyama; Shin-ichi Miyata; Masashi Ugaki and Shigetou Namba, The genomes of most prokaryotic and eukaryotic single-stranded (ss) DNA viruses, and some prokaryotic plasmids such as pLS1, commonly replicate via a rolling circle replication (RCR) strategy, and thus the viruses are hypothesized to have evolved from the plasmids, although evidence for this view is sparse. We have sequenced a circular plasmid of 3933 nt, pOYW, obtained from onion yellows phytoplasma (OY-W), a cell-wall-less, unculturable prokaryote that inhabits the cytoplasm of both plant and insect cells. pOYW contains five open reading frames (ORFs) on the same strand and apparently replicates by an RCR mechanism. Its rep gene (ORF5) encodes a unique protein, pOYW-Rep, with an unprecedented structure. The N-terminal region of pOYW-Rep has similarities to the RCR initiator protein (Rep) of pLS1 family plasmids but, unlike the Rep of other plasmids, its C;terminal region was unexpectedly similar to the helicase domain of the replication-associated proteins (Rap) of eukaryotic viruses, especially circoviruses (ssDNA viruses of vertebrates). The pOYW-Rep was specifically detected in OY-W-infected plant phloem cells, suggesting that it is a functional protein. We suggest that an ancestral phytoplasma plasmid pOYW may have acquired a helicase domain from host phytoplasmal DNA, entered the surrounding eukaryotic cytoplasm, and subsequently evolved into an ancestral eukaryotic ssDNA virus. Alternatively, a pOYW ancestor could have obtained the helicase domain by recombination with a virus: this would be the first example of recombination between plasmids and viruses. (C) 2001 Academic Press., ACADEMIC PRESS INC
Virology, 2001年, ［査読有り］

Cloning and Expression Analysis of Phytoplasma Protein Translocation Genes
Shigeyuki Kakizawa; Kenro Oshima; Tsutomu Kuboyama; Hisashi Nishigawa; Hee-young Jung; Toshimi Sawayanagi; Tsuneo Tsuchizaki; Shin-ichi Miyata; Masashi Ugaki; and Shigetou Namba, Genes encoding SecA and SecY proteins, essential components of the Sec protein translocation system, were cloned from onion yellows phytoplasma, an unculturable plant pathogenic bacterium. The secA gene consists of 2,505 nucleotides encoding an 835 amino acid protein (95.7 kDa) and shows the highest similarity with SecA of Bacillus subtilis. Anti-SecA rabbit antibody was prepared from a purified partial SecA protein, with a histidine tag expressed in Escherichia coli. Western blot analysis con. firmed that SecA protein (approximately 96 kDa) is produced in phytoplasma-infected plants. Immunohistochemical thin sections observed by optical microscopy showed that SecA is characteristically present in plant phloem tissues infected with phytoplasma. The secY gene consists of 1,239 nucleotides encoding a 413 amino acid protein (45.9 kDa) and shows the highest similarity with SecY of B. subtilis. These results suggest the presence of a functional Sec system in phytoplasmas. Because phytoplasmas are endocellular bacteria lacking cell walls, this system might secrete bacterial proteins directly into the host cytoplasm. This study is what we believe to be the first report of the sequence and expression analysis of phytoplasma genes encoding membrane proteins with a predicted function., AMER PHYTOPATHOLOGICAL SOC
Molecular Plant-Microbe Interactions, 2001年, ［査読有り］

Histopathology and immunological detection of an onion yellows phytoplasma with derivative lines: Lines producing milder symptoms, including a non-insect-transmissible line, lack hyperplastic phloem　　　　　　　　　　　　　　　
Kenro Oshima; Toshiki Shiomi; Tsutomu Kuboyama; Toshimi Sawayanagi; Hisashi Nishigawa; Shigeyuki Kakizawa; Shin-ichi Miyata; Masashi Ugaki; Shigetou Namba
Phytopathology, 2001年, ［査読有り］

Genomic factors responsible for abnormal morphology of pollen tubes in the interspecific cross, Nicotiana tabacum × N. rustica
Tsutomu Kuboyama; Ggenkichi Takeda, 筆頭著者, Nicotiana tabacum shows unilateral pollen-pistil incongruity with N. rustica. If N. tabacum is pollinated with N. rustica, growth of the pollen tube is arrested in the middle of the style, and abundant callose deposition, tube swelling and tube winding are observed. An attempt was made to clarify the genomic factors responsible for this pollen-pistil incongruity. N. tabacum was pollinated with hi paniculata or N. undulata, progenitors of amphidiploid N. rustica. When pollinated with N. undulata, growth of the pollen tube was arrested in the middle of the style and showed abnormal morphology similar to that with N. rustica, but when pollinated with N. paniculata the pollen tube reached near the base of the style and was almost normal in appearance. These observations suggest that the factors responsible for the pollen tube abnormality of N. rustica are derived from the N. undulata genome. We also used N. sylvestris, N. glutinosa and N. otophora as pistilar parents and N. rustica or its progenitors as pollen parents to examine the genomic factors of the pistilate parents. The pollen tube features of these three species in the Distils of N. sylvestris were similar to those in the pistil of N. tabacum., SPRINGER VERLAG
Sexual Plant Reproduction, 2000年, ［査読有り］

ファイトプラズマより分離された染色体外DNAの解析　　　　　　　　　　　　　　　
大島研郎・各務 孝・久保山勉・金沢登紀子・西川尚志・澤柳利実・田中 穣・松田 泉・土崎常男・難波成任
日本マイコプラズマ学会雑誌, 1999年, ［査読有り］

ファイトプラズマのチミジル酸キナーゼ遺伝子の解析
甲本晃啓・大島研郎・金沢登紀子・澤柳利実・荒井 諭・松田 泉・土崎常男・久保 山 勉・難波成任, 日本マイコプラズマ学会事務局
日本マイコプラズマ学会雑誌, 1999年, ［査読有り］

OY-ファイトプラズマ病徴変異株の分子生物学的解析
金沢登紀子, 澤柳利実,田中穣,西川尚志,久保山勉,荒井諭,大島研郎,松田泉,土崎常男,難波成任
日本マイコプラズマ学会雑誌, 1998年, ［査読有り］

OY-Wファイトプラズマより分離されたRCR型プラスミドpOYW1
久保山勉; 魯暁云,澤柳利実,金沢登紀子,西川尚志,荒井諭,大島研郎,松田泉,土崎常男,難波成任, 筆頭著者
日本マイコプラズマ学会雑誌, 1998年, ［査読有り］

A plasmid isolated from phytopathogenic onion yellows phytoplasma and its heterogeneity in the pathogenic phytoplasma mutant
Tsutomu Kuboyama; Chieh-Chen Huang; Xiaoyun Lu; Toshimi Sawayanagi; Tokiko Kanazawa; Takashi Kagami; Izumi Matsuda; Tsuneo Tsuchizaki; and Shigetou Namba, 筆頭著者, A 3.6-kbp DNA fragment was cloned from the extrachromosomal DNA of a pathogenic plant mollicute, onion yellows phytoplasma (OY-W), Sequence analysis of the fragment revealed an open reading frame (ORF) encoding the replication (Rep) protein of rolling-circle replication (RCR)-type plasmids. This result suggests the existence of a plasmid (pOYW1) in OY-W that uses the RCR mechanism. This assumption was confirmed by detecting the single-stranded DNA (ssDNA) of a replication intermediate that is specifically produced by the RCR mechanism. This is the first report on the identification of the replication system of this plasmid and the genes encoded in it. With a DNA fragment including the Rep gene region of pOYW1 used as a probe, Southern and Northern (RNA) blot hybridizations were employed to examine the heterogeneity between the plasmids found in OY-W and a pathogenic mutant (OY-M) isolated from OY-W. Multiple bands were detected in the DNA and RNA extracted from both OY-W and OY-M infected plants, although the banding patterns were different. Moreover, the copy number of plasmids from OY-W was about 4.2 times greater than that from OY-M. These results indicate constructive heterogeneity between OY-W and OY-M plasmids, and the possibility of a relationship between the plasmid-encoded genes and the pathogenicity of the phytoplasma was suggested., AMER PHYTOPATHOLOGICAL SOC
Molecular Plant-Microbe Interactions, 1998年, ［査読有り］

A novel thaumatin-like protein gene of tobacco is specifically expressed in the transmitting tissue of stigma and style
Tsutomu Kuboyama, 筆頭著者, A cDNA clone that encodes a novel thaumatin-like glycosylated protein, SE39b, which constitutes one of the major proteins of the stigmatic exudate of tobacco, was isolated and sequenced. The deduced amino acid sequence of SE39b shows 37% identity with the pathogenesis-related protein of tobacco, PR-R1, and 52% identity with the thaumatin-like protein (TLP) of Arabidopsis. All 16 cysteine residues are conserved in SE39b as they are in all TLPs. Three potential glycosylation sites found in SE39b were consistent with a previous finding that concanavalin A has an affinity for SE39b. Northern blot and in situ analyses demonstrated that the gene was specifically expressed in the transmitting tissue of the stigma and style, and the transcript amounts reached maximum levels at anthesis. mRNA from orthologs of the gene of SE39b was detected in species of Cestreae, but not in species of Solaneae. SE39b should be categorized as a PR-like glycoprotein which is developmentally regulated and specifically expressed in the transmitting tissue of the stigma and style, such as Sp41 [ (1,3)-beta-glucanase], SK2 and Chi2;1 (chitinase)., SPRINGER VERLAG
Sexual Plant Reproduction, 1998年, ［査読有り］

An acidic 39-kDa protein secreted from stigmas of tobacco has an amino-terminal motif that is conserved among thaumatin-like proteins
Tsutomu Kuboyama; Kaoru T. Yoshida; Genkichi Takeda, 筆頭著者, An acidic 39-kDa polypeptide (SE39b) secreted from stigmas of tobacco was identified by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and its sugar chain was detected with concanavalin A (Con A) and peroxidase. The amino-terminal amino acid sequence of SE39b resembled those of thaumatin-like proteins., JAPANESE SOC PLANT PHYSIOLOGISTS
Plant & Cell Physiology, 1997年, ［査読有り］

Isolation of sexual reproduction-related genes in rice by simplified differential display. Rice Genetics NewsLetter　　　　　　　　　　　　　　　
Yoshida, K.T; Kuboyama, T
Rice Genetics NewsLetter, 1997年, ［査読有り］

The diversity of interspecific pollen-pistil incongruity in Nicotiana
T. Kuboyama; C. S. Chung and G. Takeda, 筆頭著者, Nicotiana tabacum was used as a pistillate parent and crossed with three self-compatible species, N. rustica, N. repanda and N. trigonophylla, which were previously reported to have pollen tubes unilaterally inhibited by N. tabacum pistil. Temporal and morphological observations revealed distinct differences of pollen tube behavior among these incongruous crosses. Pollen tubes of N. repanda were arrested in stigma and those of N. rustica in the middle of the style. On the other hand, pollen tubes of N. trigonophylla continued growing at a slow rate. Tubes of N. repanda and N. rustica showed morphological abnormalities such as swelling, thick wall, and irregular callose deposition. In addition, tubes of N. rustica often elongated in reverse direction and wound about in the middle of the style. Although the tubes of N. trigonophylla were apparently normal in morphology, they were distributed throughout the transmitting tissue, differing from the self-pollination of N. tabacum in which they were confined to the peripheral region of it. The diversity of pollen tube behavior indicates that physiological causes of incongruity are different among the three crosses. Bud pollination enabled pollen tubes to reach the ovary in all crosses, indicating that the N. tabacum pistil acquired its ability to inhibit foreign pollen tube elongation with its development. When interspecific hybrids between N. tabacum and the other three species were pollinated by parental species, tubes reached the ovary in all crosses, but the elongation rate of tubes slowed down and morphology was abnormal., SPRINGER VERLAG
Sexual Plant Reproduction, 1994年, ［査読有り］

食味と穀粒成分および炊飯米のアミノ酸との関係
松崎昭夫・高野哲夫・坂本晴一・久保山勉
日本作物学会紀事, 1992年, ［査読有り］

HYBRID PLANT OF PHASEOLUS-VULGARIS L AND P LUNATUS L OBTAINED BY MEANS OF EMBRYO RESCUE AND CONFIRMED BY RESTRICTION ENDONUCLEASE ANALYSIS OF RDNA
T KUBOYAMA; Y SHINTAKU; G TAKEDA, Hybridizations between three lines of the common bean (Phaseolus vulgaris L.) and two lines of the lima bean (P. lunatus L.) were attempted in order to transfer characters from the lima bean to the common bean. A total of 115 interspecific hybrid embryos were rescued and cultured, and 7 plantlets were eventually transferred to soil. The most compatible cross was 'Edogawa XPI164891', which had a high proportion of expanded ovules, and from which we obtained one mature interspecific hybrid. In general, morphological characters of the hybrid were intermediate between the parents, and the chromosome number of the hybrid was 2n = 22 the same as that of both parents. The hybrid nature of the progeny plant was confirmed by restriction endonuclease analysis of rDNA. Species specific fragments were detected when total DNA was digested by BamHI, and BamHI-digested total DNA of the hybrid contained all fragments from both parents. Selves and backcrosses were attempted, but no progeny were obtained. The only hybrid obtained was completely sterile and meiosis highly irregular., KLUWER ACADEMIC PUBL
EUPHYTICA, 1991年04月, ［査読有り］

Cross-species amplification of 349 melon (Cucumis melo L.) microsatellites in gherkin (Cucumis anguria L.) .
Matsumoto Y; Watanabe N; Kuboyama T
J Plant Breed Crop Sci, 2012年

アサガオ(Ipomoea nil)由来EST‐SSRを応用したサツマイモのゲノム解析
岡田吉弘; 久保山勉; 星野敦; 飯田滋; 仁田坂英二; 福岡浩之; 吉永優
九州農業研究発表会専門部会発表要旨集, 2011年08月

Nicotiana debneyiはN.tabacumとの交雑における雑種致死の原因となる単一の優性遺伝子をもつ
飯塚貴大; 久保山勉; 丸橋亘; 小田雅行; 手塚孝弘
育種学研究, 2011年

ラッカセイ栽培種におけるEST由来SSRマーカーの開発
内藤嘉磯; 鈴木茂; 長谷川誠; 渡邉学; 久保山勉; 磯部祥子; 佐藤修正; 白澤健太; 笹本茂美; 田畑哲之
育種学研究, 2009年09月25日

Genetic mapping of a mutant gene producing three pistils in a floret of common wheat
Peng, Z.-S; Martinek, P; Kosuge, K; Kuboyama, T; Watanabe, N
J. Appl. Genet., 2008年

ジャポニカ型イネと品種「Jamaica」の間で見られる雑種弱勢 (日本の遺伝学の潮流--日本遺伝学会第78回大会ハイライト) -- (ワークショップ 栽培植物の遺伝学--雑種形成,倍数性進化,多様性)
久保山 勉; 一谷 勝之
生物の科学「遺伝」 別冊, 2007年03月

Genetic mapping of the genes and development of near-isogenic lines in durum wheat
N. Watanabe; K. Kosuge; T. Kuboyama
European Wheat Aneuploid Co-operative Newslettter 2008, 2007年

The hybrid weakness of the cross between Japanese rice cultivar "Nipponbare" and Peruvian rice cultivar "Jamaica"
Tsutomu Kuboyama
GENES & GENETIC SYSTEMS, 2006年12月

Cloning of oligopeptide permiase homologues from OY phytoplasma genome (第28回日本マイコプラズマ学会学術集会)
宮田 伸一; Furuki Ken-ichiro; Sawayanagi Toshimi; OSHIMA Kenro; KUBOYAMA Tsutomu; UGAKI Masashi; NAMBA Shigetou
日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 2001年12月28日

(365)ファイトプラズマのoligopeptide transport system遺伝子群のクローニング
宮田 伸一; 古木 健一朗; 甲本 晃啓; 澤柳 利実; 大島 研郎; 久保山 勉; 宇垣 正志; 土崎 常男; 難波 成任
日本植物病理學會報, 2001年08月25日

ファイトプラズマのS10-spc ribosomal protein gene operonの遺伝子構成と開始/終止コドンは動物マイコプラズマよりも枯草菌に似ている(関東部会講演要旨)
古木 健一朗; 甲本 晃啓; 澤柳 利実; 大島 研郎; 久保山 勉; 宮田 伸一; 宇垣 正志; 土崎 常男; 難波 成任
日本植物病理學會報, 2000年12月25日

ファイトプラズマの野生株及び弱毒変異株より分離された染色体外DNAの解析
西川尚志; 大島研郎; 久保山勉; 沢柳利実; 宮田伸一; 宇垣正志; 難波成任
日本分子生物学会年会プログラム・講演要旨集, 2000年11月25日

ファイトプラズマより分離されたプラスミドの構造と複製蛋白質の感染植物における発現解析
大島研郎; 柿沢茂行; 西川尚志; 久保山勉; 宮田伸一; 宇垣正志; 難波成任
日本分子生物学会年会プログラム・講演要旨集, 2000年11月25日

ファイトプラズマのプラスミド複製関連遺伝子(rep)の感染植物における発現
金沢 登紀子; 久保山 勉; 柿澤 茂行; 西川 尚志; 大島 研郎; 松田 泉; 土埼 常男; 難波 成任
日本植物病理學會報, 2000年08月25日

ファイトプラズマの野生株及び変異株より分離されたプラスミドの比較構造解析
大島 研郎; 西川 尚志; 久保山 勉; 松田 泉; 土埼 常男; 難波 成任
日本植物病理學會報, 2000年08月25日

ファイトプラズマのelongation factor-G/Tu遺伝子の構成は動物mycoplasmaよりも枯草菌に近い
宮田 伸一; 古木 健一朗; 澤柳 利実; 大島 研郎; 久保山 勉; 宇垣 正志; 松田 泉; 土埼 常男; 難波 成任
日本植物病理學會報, 2000年08月25日

ファイトプラズマのthymidylate kinase遺伝子の解析
甲本 晃啓; 古木 健一朗; 澤柳 利実; 大島 研郎; 宮田 伸一; 久保山 勉; 宇垣 正志; 松田 泉; 土埼 常男; 難波 成任
日本植物病理學會報, 2000年08月25日

ファイトプラズマの染色体外DNAはローリングサークル型の複製を行うプラスミドである
大島研郎; 西川尚志; 金沢登紀子; 沢柳利実; 甲本晃啓; 松田泉; 土崎常男; 久保山勉; 難波成任
日本分子生物学会年会プログラム・講演要旨集, 1999年11月22日

植物DNAウイルス型複製酵素を持ったファイトプラズマの大型染色体外DNA
西川尚志; 大島研郎; 久保山勉; 金沢登紀子; 柿沢茂行; 田中穣; 松田泉; 土崎常男; 難波成任
日本分子生物学会年会プログラム・講演要旨集, 1999年11月22日

(189) ファイトプラズマより分離された染色体外DNAの解析 (平成11年度 日本植物病理学会大会)
大島 研郎; 各務 孝; 久保山 勉; 金沢 登紀子; 西川 尚志; 澤柳 利実; 田中 穣; 松田 泉; 土崎 常男; 難波 成任
日本植物病理學會報, 1999年06月25日

アサガオのアフリカ系統 Q63 とアメリカアサガオ Q65 の F2 分離集団における早咲き原因 QTL の探索　　　　　　　　　　　　　　　
村上 喜一; 星野 敦; 仁田坂 英二; 久保山 勉
日本育種学会第147回講演会, 2025年03月21日
20250320, 20250321

J-PARC加速器の中性子線照射によって得られたアルビノ変異体の原因となる染色体変異　　　　　　　　　　　　　　　
中山 好乃; 小島 健太; 菊池 伯夫; 久保山 勉
日本育種学会第147回講演会, 2025年03月21日
20250320, 20250321

放射線による突然変異誘発と原因遺伝子の探索について　　　　　　　　　　　　　　　
久保山勉
アサガオ研究集会, 2024年10月14日
20241013, 20241014

Asuを1 回親，台中 65号を反復親にしたBC1F1における雑種不稔・収量関連形質のQTL解析　　　　　　　　　　　　　　　
荻原周平; 仲村洋輔; 小城太紀; 坂本龍弥; 一谷勝之; 浅木直美; 久保山勉
日本育種学会第146回講演会, 2024年09月19日
20240919, 20240920

アサガオにおいて花筒の着色に関与するC1(InMYB1)遺伝子の重複　　　　　　　　　　　　　　　
仁田坂英二; 星野敦; 久保山勉; 白澤健太
日本植物学会大会, 2024年09月
20240913, 20240916

アサガオの短日性花成誘導機構におけるBBX転写因子の機能解析　　　　　　　　　　　　　　　
楊一寧; 小野公代; 鷲塚澪; 大野唯; 久保山勉; 星野敦; 星野敦; 仁田坂英二; 白澤健太; 樋口洋平; 小野道之
日本植物学会大会, 2024年09月
20240913, 20240916

BC1F1世代を用いたイネ品種台中65号とKasalathとの間の雑種第一代に見られる雑種強勢のQTL解析　　　　　　　　　　　　　　　
仲村 洋輔,荻原 周平,一谷 勝之,久保山 勉
日本育種学会第145回講演会, 2024年03月17日, 日本育種学会
20240316, 20240317

イネ雑種の初期成育においてバイサルファイトアンプリコンシーケンスで検出された 18S rDNA のメチル化レベルのヘテロシス　　　　　　　　　　　　　　　
大槻 日向子,仲村 洋輔,一谷 勝之,久保山 勉
日本育種学会第145回講演会, 2024年03月17日, 日本育種学会
20240316, 20240317

アサガオが温帯アジアに分布するのに必要だった と推定される InCO のフレームシフト変異　　　　　　　　　　　　　　　
勝山 弘章; 江面 恭佑; 星野 敦; 仁田坂 英 二; 久保山 勉
日本育種学会第145回講演会, 2024年03月17日, 日本育種学会
20240316, 20240317

J-PARC加速器を用いた中性子線照射イネのM2世代における全ゲノムリシーケンス解析　　　　　　　　　　　　　　　
小島 健太,石橋 佳奈,菊池 伯夫,高妻 孝光,星川 晃範,久保山 勉
日本育種学会第144回講演会, 2023年09月17日, 日本育種学会
20230916, 20230917

イネ雑種の初期生育において 18S rDNA の 5′領域に見られたメチル化レベルの上昇　　　　　　　　　　　　　　　
日本育種学会第144回講演会, 2023年09月17日, 日本育種学会
20230916, 20230917

γ 線照射により作出されたアサガオ新規白花突然変異体　　　　　　　　　　　　　　　
荷見 円; 岡野 凌平; 勝山 弘章; 高橋 悠愛佳; 水野 貴行; 星野 敦; 仁田坂 英二; 久保山 勉
日本育種学会第143回講演会, 2023年03月18日, 日本育種学会
20230317, 20230318

アサガオ早生系統北京天壇の到花日数を変化させ る晩生系統 Q63 染色体領域の探索　　　　　　　　　　　　　　　
舟川 奈那; 勝山 弘章; 久保山 勉
日本育種学会第143回講演会, 2023年03月18日, 日本育種学会
20230317, 20230318

WRC 系統と台中 65 号の雑種におけるイネ初期生 育ヘテロシスの GWAS と BC1F1 を用いた QTL 解析　　　　　　　　　　　　　　　
仲村 洋輔; 一谷 勝之; Matthew Shenton; 田中 伸裕; 久保山 勉
日本育種学会第143回講演会, 2023年03月17日, 日本育種学会
20230317, 20230318

コシヒカリと Oryza rufipogon W1944 の F2 集団における柱頭露出率のQTL解析　　　　　　　　　　　　　　　
高瀬 ゆうの; 久保山 勉
日本育種学会第143回講演会, 2023年03月17日, 日本育種学会
20230317, 20230318

γ線照射によって得られたアサガオ新規白花変異体　　　　　　　　　　　　　　　
山村 龍; 岡野 凌平; 勝山 弘章; 高橋 悠愛佳; 水野 貴行; 星野 敦; 仁田坂 英二; 久保山 勉
日本育種学会第142回講演会, 2022年09月24日, 日本育種学会
20220923, 20220924

J-PARC加速器中性子線の種子照射によるイネの突然変異誘発　　　　　　　　　　　　　　　
小島 健太; 石橋 佳奈; 及川 健一; 勝山 弘章; 菊池 伯夫; 高妻 孝光; 原田 正英; 星川 晃範; 久保山 勉
日本育種学会第142回講演会, 2022年09月24日, 日本育種学会
20220923, 20220924

世界イネコアコレクションと台中 65 号の雑種における初期生育ヘテロシスの検討　　　　　　　　　　　　　　　
仲村 洋輔,一谷 勝之,Shenton Matthew,田中 伸裕,久保山 勉
日本育種学会第142回講演会, 2022年09月24日, 日本育種学会
20220923, 20220924

リアルタイム PCR を用いた日本晴と Kasalath のイネ品種間雑種幼苗期におけるオルガネラゲノムと 18S rDNA のコピー数の検討　　　　　　　　　　　　　　　
高間 梨央 ,一谷 勝之 ,久保山 勉
日本育種学会第139回講演会, 2021年03月21日, 日本育種学会

Mapping of qIF1, a QTL for days to flowering of morning glory and 24h expression of InCO, a candidate gene of qIF3　　　　　　　　　　　　　　　
Keeranon Sasiprapa; Katsuyama Hiroaki; Ono Mitiyuki; Kuboyama Tsutomu
日本育種学会第138回講演会, 2020年10月11日, 日本育種学会

日本晴と Kasalath のイネ品種間交雑 F2 集団にお けるプラスチドゲノムコピー数と生重量の QTL 解析　　　　　　　　　　　　　　　
☆高間 梨央 1; 白澤 健太 2; 一谷 勝之 3; 安達 俊 輔 1; 田中 淳一 4; 久保山 勉
日本育種学会第138回講演会, 2020年10月11日, 日本育種学会

アサガオにおける γ 線を用いた突然変異誘発の試み　　　　　　　　　　　　　　　
岡野 凌平,高橋 悠愛佳,勝山 弘章,久保山 勉
日本育種学会第138回講演会, 2020年10月11日, 日本育種学会

オセアニア産 AA ゲノム野生イネの形態，生理学，遺伝的多様性　　　　　　　　　　　　　　　
萩山 勇希; 佐藤 雅志; ヘンリー ロバート; 久保山 勉; 田浦 悟; 石川 隆二; 一谷 勝之
日本育種学会第138回講演会, 2020年10月10日, 日本育種学会

イネ品種日本晴と Kasalath の正逆交雑によって得 られた雑種の生育初期に見られるヘテロシスと光合成速度　　　　　　　　　　　　　　　
高間 梨央; 安達 俊輔; 一谷 勝之; 田中 淳一; 久保山 勉
日本育種学会第137回講演会, 2020年03月29日

日本晴とKasalathのイネ品種間交雑F₂集団におけるプラスチドゲノムコピー数と生重量のQTL解析　　　　　　　　　　　　　　　
高間梨央; 白澤健太; 一谷勝之; 安達俊輔; 田中淳一; 久保山勉
育種学研究, 2020年
2020, 2020

レンコンの種ハスにおける混種確認手法の開発　　　　　　　　　　　　　　　
堀井学 ・篠原啓子 ・白澤健太 ・久保山勉 ・樋口洋平 ・八城和敏
園芸学会平成30年度春季大会, 2019年09月16日, 園芸学会

花ハス遺伝資源のレンコン形質に関する多様性解析と優良系統の探索　　　　　　　　　　　　　　　
松澤陸斗 ・石川祐聖 ・工藤新司 ・白澤健太 ・久保山勉 ・堀井学 ・柴田道夫 ・樋口洋平
園芸学会平成30年度春季大会, 2019年09月16日, 園芸学会

日本晴とJamaicaのイネ雑種弱勢におけるプラスチドゲノムコピー数の低下　　　　　　　　　　　　　　　
中沢 裕哉,青木 梨乃,一谷 勝之,久保山 勉
日本育種学会第136回講演会, 2019年09月07日, 日本育種学会

日本晴と Kasalath のイネ品種間雑種における幼苗期の雑種強勢とプラスチドゲノムコピー数の増大　　　　　　　　　　　　　　　
高間 梨央 ,田中 淳一 ,一谷 勝之 ,久保山 勉
日本育種学会第136回講演会, 2019年09月07日, 日本育種学会

コシヒカリ・日本晴間で検出された穂首抽出長に関するQTLのファインマッピング　　　　　　　　　　　　　　　
石川 春香; 赤坂 舞子; 堀 清純; 久保山 勉; 田中 淳一
日本育種学会第136回講演会, 2019年09月06日, 日本育種学会

HRM法によるレンコンの簡易品種識別法の開発　　　　　　　　　　　　　　　
仮屋 亜由美 1; 堀井 学 2; 白澤 健太 3; 篠原 啓子 4; 澤田 英司 4; 八城 和敏 2; 樋口 洋平 5; 石川 祐聖 5; 井上 栄一 1; 久保山 勉 1
日本育種学会第135回講演会, 2019年03月17日, 日本育種学会

HRM法によるレンコンの簡易品種識別法の開発
仮屋亜由美; 堀井学; 白澤健太; 篠原啓子; 澤田英司; 八城和敏; 樋口洋平; 石川祐聖; 井上栄一; 久保山勉
育種学研究, 2019年03月16日
20190316, 20190316

InCOにおけるsplicing variantの日長条件による量的変化　　　　　　　　　　　　　　　
勝山 弘章; 小野 道之; 久保山勉
第10回アサガオ研究集会, 2019年03月10日

レンコンの品種識別に用いるダイレクト PCR 手法の検討　　　　　　　　　　　　　　　
○堀井学,葛谷真輝,久保山勉,白澤健太,八城和敏
園芸学会平成30年度秋季大会, 2018年09月23日, 園芸学会

「生殖隔離の克服によるCucumis属野生種とメロンとの種間雑種作出の試 み」　　　　　　　　　　　　　　　
松本 雄一 1,久保山 勉 2 (1. 佐賀 大・農,2. 茨城大・農)
日本育種学会第134回講演会, 2018年09月22日, 日本育種学会

イネ雑種弱勢の遺伝と病徴　　　　　　　　　　　　　　　
一谷 勝之,久保山 勉,手塚 孝 弘
日本育種学会第134回講演会, 2018年09月22日, 日本育種学会

ハス(レンコン)の混合サンプルからの定量的品 種識別法の開発　　　　　　　　　　　　　　　
白澤 健太; 久保山 勉; 堀井 学; 樋口 洋平; 磯部 祥子
日本育種学会第134回講演会, 2018年09月22日, 日本育種学会

CRISPR/Cas9を用いたアサガオの光周性花成誘導におけるCONSTANSの機能解析　　　　　　　　　　　　　　　
鷲塚澪; 小野公代; 渡邊健太; 本山星香; 樋口洋平; 久保山勉; 白澤健太; 小野道之
日本植物学会第82回広島大会, 2018年09月15日, 日本植物学会

午後開花アサガオにおける開花時刻のQTL解析　　　　　　　　　　　　　　　
本山 星香,小野 公代,鷲塚 澪,甲斐 文子,遠藤 沙織,渡邊 健太,久保山 勉,中嶋 信美,白澤 健太,小野 道之
日本植物学会第82回広島大会, 2018年09月15日, 日本植物学会

ddRAD-seq 解析で得られた SNP に基づくレンコン品種識別法の種レンコン生産への利用　　　　　　　　　　　　　　　
篠原啓子 ・白澤健太 ・堀井学 ・樋口洋平 ・澤田英司 ・八城和俊 ・井上栄一 ・久保山勉
園芸学会中四国支部発表会, 2018年07月21日, 園芸学会中四国支部

Reproductive barriers in the interspecific hybridization between Ipomoea nil and I. hederacea　　　　　　　　　　　　　　　
Yui Hozumi; Maki Noguchi; Eiji Nitasaka; Tsutomu Kuboyama
The 25th International Congress on Sexual Plant Reproduction, 2018年06月11日, The International Association of Sexual Plant Reproduction Research (IASPRR)

ddRAD-seq解析より得られたSNPに基づくレンコン品種識別法の開発　　　　　　　　　　　　　　　
小林 希美 1; 白澤 健太 2; 堀井 学 3; 篠原 啓子 4; 澤田 英司 4; 八城 和敏 3; 樋口 洋平 5; 石川 祐聖 5; ハク エムダドウル 1; 井上 栄一 1; 久保山 勉 1 (1 茨大農; 2 かずさDNA研; 3 茨城農総セ生工研; 4 徳島農総技セ; 5 東大農)
日本育種学会第133回講演会, 2018年03月26日, 日本育種学会

花ハス根茎におけるポリフェノール含量の多様性　　　　　　　　　　　　　　　
ハクエムダドウル、樋口洋平、石川祐聖、堀井学、八城和敏、井上栄一、久保山勉
日本育種学会第133回講演会, 2018年03月26日

PacBioシーケンサーリードによるイネ雑種弱勢原因遺伝子HWA1領域contig作成の試み　　　　　　　　　　　　　　　
久保山勉1、一谷勝之2 (1茨城大学農学部、2鹿児島大学農学部)
日本育種学会第133回講演会, 2018年03月26日

イネ雑種弱勢原因遺伝子 HWA1, HWA2の連鎖分析ならびに量的効果　　　　　　　　　　　　　　　
☆豊元 大希 1,田浦 悟 2,久保山 勉 3,一谷 勝之 1; 4(1. 鹿大・院連合農学,2. 鹿大・遺伝子実験施設,3. 茨大・農学,4. 鹿大・農学)
日本育種学会第133回講演会, 2018年03月26日

InDelおよびSSRマーカーを用いた国内産食用ハスの系統解析　　　　　　　　　　　　　　　
堀井学、白澤健太、久保山勉、樋口洋平、八城和敏
園芸学会平成30年度春季大会, 2018年03月26日, 園芸学会

ddRAD‐seq解析で得られたSNPに基づくレンコン品種識別法の種レンコン生産への利用
篠原啓子; 白澤健太; 堀井学; 樋口洋平; 澤田英司; 八城和俊; 井上栄一; 久保山勉
園芸学会中四国支部研究発表要旨, 2018年
2018, 2018

アサガオの到花日数 QTL 候補遺伝子 PnCO の発 現解析　　　　　　　　　　　　　　　
勝山 弘章; 小野 道之; 久保山 勉
日本育種学会第132回講演会, 2017年10月08日, 日本育種学会

イネにおける HWA1 と HWA2 および HWC1 と HWC2 による雑種弱勢の比較解析　　　　　　　　　　　　　　　
白柿 薫平; 佐原 敏基; 一谷 勝之; 久保山 勉; 松村 篤 1 森川 利信; 手塚 孝弘
日本育種学会第132回講演会, 2017年10月07日, 日本育種学会

高温条件が HWA1 と HWA2 を原因とするイネ雑種 弱勢の表現型と遺伝子発現に及ぼす影響　　　　　　　　　　　　　　　
☆小幡 正彬 1; 白柿 薫平 1; 一谷 勝之 2; 久保山 勉 3; 松村 篤1; 森川 利信1; 横井 修司1; 手塚 孝弘(1 1.大 阪府大院生命環境,2. 鹿児島大農,3. 茨城大農)
日本育種学会第132回講演会, 2017年10月07日, 日本育種学会

PacBio シーケンサーリードによるイネ雑種弱勢原 因遺伝子 HWA2 領域 contig 作成の試み　　　　　　　　　　　　　　　
鈴木 大和; 一谷 勝之; 渡部 信義; 久保山 勉
日本育種学会第131回講演会, 2017年03月30日, 日本育種学会

アサガオとアメリカアサガオの種間交雑における結実率の系統間差　　　　　　　　　　　　　　　
野口 真希,八月朔日 優衣,仁田坂 英二,渡辺 信義,久保山 勉
日本育種学会第131回講演会, 2017年03月30日, 日本育種学会

Hwa1-1と Hwa2-1の補足作用によって引き起こされる雑種弱勢現象の系統間差異　　　　　　　　　　　　　　　
保木 良太,植村 真郷,田浦 悟,久保山 勉,一谷 勝之
日本育種学会第131回講演会, 2017年03月30日, 日本育種学会

イネにおけるHWA1とHWA2およびHWC1とHWC2による雑種弱勢の比較解析　　　　　　　　　　　　　　　
白柿薫平; 佐原敏基; 一谷勝之; 久保山勉; 松村篤; 森川利信; 手塚孝弘
育種学研究, 2017年
2017, 2017

RAD-seqを用いた、アサガオ開花早晩性ＱＴＬの検出　　　　　　　　　　　　　　　
勝山弘章、奥野菫、白澤健太、仁田坂英二、星野敦、小野道之、渡部信義、久保山勉、境科
日本育種学会第129回講演会, 2016年03月22日, 日本育種学会

アサガオ花色の濃さQTL間相互作用とADM2候補遺伝子InMYB1における系統間塩基配列多型　　　　　　　　　　　　　　　
宮本香、奥野菫、勝山弘章、星野敦、仁田坂英二、飯田滋、渡部信義、久保山勉
日本育種学会第129回講演会, 2016年03月22日, 日本育種学会

生物研世界イネコアコレクションにおける雑種弱 勢遺伝子 Hwa1-1, Hwa2-1 の分布　　　　　　　　　　　　　　　
一谷 勝之; 古賀 功明; 坂口 瑛進; 田浦 悟; 石川隆二; 手塚孝弘; 久保山勉
日本育種学会第127回講演会, 2015年03月22日

イネ雑種弱勢原因遺伝子 Hwc1-1の原因 SNP による HWC1 タンパク質間親和性の低下　　　　　　　　　　　　　　　
沖山 友哉; 一谷 勝之; 渡部 信義; 久保山 勉
日本育種学会第127回講演会, 2015年03月22日, 日本育種学会

HWA1と HWA2によって生じるイネ雑種弱勢の遺伝子発現解析　　　　　　　　　　　　　　　
白柿 薫平; 一谷 勝之; 久保山 勉; 簗瀬 雅則; 森川 利信; 手塚 孝弘
日本育種学会第127回講演会, 2015年03月22日, 日本育種学会

コムギにおける screwed spike rachis 遺伝子のマッピング　　　　　　　　　　　　　　　
雨谷 弓弥子; Martinek Petr; 久保山 勉; 渡部 信義
日本育種学会第127回講演会, 2015年03月22日, 日本育種学会

HWA1とHWA2によって生じるイネ雑種弱勢の遺伝子発現解析　　　　　　　　　　　　　　　
白柿薫平; 一谷勝之; 久保山勉; 簗瀬雅則; 森川利信; 手塚孝弘
育種学研究, 2015年
2015, 2015

生物研世界イネコアコレクションにおける雑種弱勢遺伝子Hwa1-1,Hwa2-1の分布　　　　　　　　　　　　　　　
一谷勝之; 古賀功明; 坂口瑛進; 田浦悟; 石川隆二; 手塚孝弘; 久保山勉
育種学研究, 2015年
2015, 2015

四倍性コムギの無葉耳性に関わる遺伝子のマッピングおよび準同質遺伝子系統の育成　　　　　　　　　　　　　　　
☆雨谷 弓弥子; 久保山 勉; 渡部 信義
日本育種学会第126回講演会, 2014年09月27日, 日本育種学会

アサガオにおける到花日数QTLの検出　　　　　　　　　　　　　　　
☆勝山 弘章1、奥野 菫1、伊東 拓郎1、仁田坂 英二2、星野 敦3、飯田 滋4、小野 道之5、渡部 信義1、久保山 勉1 (1 茨城大農; 2 九州大学大学院理学研究院; 3 基生研; 4 静岡県立大生活健康科学研究院・薬学研究科; 5 筑波大生命環境科学研究科)
日本育種学会第125回講演会, 2014年03月22日, 日本育種学会

アサガオにおける花色の濃さ QTLとアントシアニン生合成関連遺伝子のマッピング　　　　　　　　　　　　　　　
奥野 菫 1; 伊東 拓朗 1; 勝山 弘章 1; 星野 敦 2; 仁田坂 英二 3; 飯田 滋 4; 渡部 信義 1; 久保山 勉 1 (1 茨城大農; 2 基生研; 3 九州大学大学院理学研究院; 4 静岡県立大生活健康科学研究科・薬学研究科)
日本育種学会第125回講演会, 2014年03月22日, 日本育種学会

タルホコムギにおける無葉耳性に関わる遺伝子のマッピングおよび合成コムギの育成　　　　　　　　　　　　　　　
◯雨谷 弓弥子; 渡部 信義; 久保山 勉(茨城大・農)
日本育種学会第125回講演会, 2014年03月22日, 日本育種学会

日本型イネとJamaicaの間で見られる雑種弱勢緩和変異体　　　　　　　　　　　　　　　
橋本陽平、一谷勝之、武田純子、沖山友哉、西村実、土師岳、渡部信義、久保山勉
第52回ガンマーフィールドシンポジウム, 2013年07月17日, 独立行政法人生物資源研究所放射線育種場

イネ雑種弱勢遺伝子HWA1, HWA2の高密度連鎖解析　　　　　　　　　　　　　　　
◯一谷 勝之 1; 浦田 千恵子 1; 田浦 悟 2; 手塚 孝弘 3; 沖山 友哉 4; 久保山 勉 4; 佐藤 宗治 1(1. 鹿大・農学; 2. 鹿 大・遺伝子実験施設; 3. 大阪府大院・ 生命環境; 4. 茨大・農学)
日本育種学会第123回講演会, 2013年03月28日, 日本育種学会

タルホコムギの突然変異遺伝子 triple glume のマッピング　　　　　　　　　　　　　　　
◯雨谷 弓弥子; 久保山 勉; 渡部 信義(茨城大・農)
日本育種学会第123回講演会, 2013年03月28日, 日本育種学会

EST-SSRとEST-SNPを用いたアサガオ連鎖地図の作成　　　　　　　　　　　　　　　
☆伊東 拓朗 1; 奥野 菫 1; 勝山 弘章 1; 森山 拳斗 1; 仁田坂 英二 2; 星野 敦 3; 飯田 滋 4; 福岡 浩之 5; 磯部 祥子 6; 佐藤 修正 6; 渡部 信義 1; 久保 山 勉 1(1. 茨城大農; 2. 九州大学大学院理学研究院; 3. 基生研; 4. 静岡県立大生活健康科学研究科・薬学研究科; 5. 農研機構・野菜茶研; 6. か ずさ DNA 研)
日本育種学会第123回講演会, 2013年03月28日

イネ雑種弱勢遺伝子HWA1,HWA2の高密度連鎖解析　　　　　　　　　　　　　　　
一谷勝之; 浦田千恵子; 田浦悟; 手塚孝弘; 沖山友哉; 久保山勉; 佐藤宗治
育種学研究, 2013年
2013, 2013

一過性発現系におけるイネ雑種弱勢原因遺伝子 HWC1の機能解析と TILLING 法を用いたHWC1 変異系統作出の試み　　　　　　　　　　　　　　　
☆沖山 友哉 1; 一谷 勝之 2; 熊丸 敏博 3; 渡部 信義 1; 久保山 勉 1(1. 茨城大・農; 2. 鹿児島大・農; 3. 九 州大院・農)
日本育種学会第122回講演会, 2012年09月14日, 日本育種学会

γ線受精卵照射によって得られた日本晴と Jamaica の雑種弱勢を緩和する変異体　　　　　　　　　　　　　　　
武田 純子 1; 五十嵐 千尋 1; 沖山 友哉 1; 西村 実 2; 一谷 勝之 3; 渡部 信義 1; ◯久保山 勉 1(1. 茨大農; 2. 生物研放育場; 3. 鹿児島大農)
日本育種学会第122回講演会, 2012年09月14日, 日本育種学会

ESTのSSRとSNPを用いたアサガオ連鎖地図の経過報告と各種QTL検出の試み　　　　　　　　　　　　　　　
○勝山弘章1、伊東拓朗1、森山拳斗1、仁田坂英二2、星野敦3、飯田滋4、福岡浩之5、小野道之6、佐藤修正7、磯部祥子7、渡部信義1、久保山勉1(1.茨城大学農学部、2.九州大学大学院理学研究院、3.基礎生物学研究所、4.静岡県立大生活健康科学研究科・薬学研究科、5.農研機構野菜茶業研究所、6.筑波大学大学院生命環境科学研究科、7.かずさDNA研究所)
第7回アサガオ研究集会, 2012年09月08日, 九州大学

青い糊粉層をもつマカロニコムギおよびパンコムギ系統の育成　　　　　　　　　　　　　　　
渡部信義1・P. Martinek2・持田実佳1・久保山勉1(1. 茨城大農2. Agrotest Fyto; Ltd.)
日本育種学会第121回講演会, 2012年03月30日, 日本育種学会

HWA1とHWA2を原因遺伝子とするイネ雑種弱勢における細胞死　　　　　　　　　　　　　　　
☆飯塚貴大1・一谷勝之2・久保山勉3・小田雅行1・手塚孝弘1(1. 大阪府大院生命環境2. 鹿児島大農3. 茨城大農)
日本育種学会第121回講演会, 2012年03月, 日本育種学会

SSRマーカーとトランスポゾンマーカーを利用したラッカセイ高密度連鎖地図の作成とQTL解析への利用　　　　　　　　　　　　　　　
○白澤健太1・P. Koilkonda1・青木考1・平川英樹1・田畑哲之1・長谷川誠2・清島浩之2・鈴木茂2・桑田主税2・内藤嘉磯3・久保山勉4・磯部祥子 1(1. かずさDNA研2. 千葉農林総研3. 三菱化学メディエンス4. 茨城大)
日本育種学会第121回講演会, 2012年03月, 日本育種学会

パーティクルガンを用いたイネ雑種弱勢原因遺伝子HWC1の一過性発現によるプログラム細胞死の誘導　　　　　　　　　　　　　　　
☆沖山友哉1・一谷勝之2・渡部信義1・久保山勉1(1. 茨大農2. 鹿児島大農)
日本育種学会第120回講演会, 2011年09月23日, 日本育種学会

イネ雑種弱勢遺伝子HWA1, HWA2の連鎖分析　　　　　　　　　　　　　　　
☆一谷勝之1・田浦悟2・手塚孝弘3・沖山友哉4・久保山勉
日本育種学会第119回講演会, 2011年03月, 日本育種学会

HWC1とHWC2によって生じるイネ雑種弱勢のマイクロアレイとGOによる遺伝子発現解析　　　　　　　　　　　　　　　
○久保山勉1・一谷勝之2・沖山友哉1・濱田和輝3・本郷耕平3・矢野健太郎3・藤田雅丈4・倉田のり4・渡部信義1(1. 茨大農2. 鹿児島大農3. 明治大農バイオインフォマ4. 遺伝研)
日本育種学会第119回講演会, 2011年03月, 日本育種学会

HWC1およびHWC2の相互作用で生じるイネ雑種弱勢における病害抵抗性関連遺伝子の発現およびファイトアレキシンの蓄積　　　　　　　　　　　　　　　
☆沖山友哉1・一谷勝之2・長谷川守文1・渡部信義1・久保山勉1(1. 茨城大学農学部2. 鹿児島大学農学部)
日本育種学会第119回講演会, 2011年03月, 日本育種学会

イネ雑種弱勢遺伝子HWA1,HWA2の連鎖分析　　　　　　　　　　　　　　　
一谷勝之; 田浦悟; 手塚孝弘; 沖山友哉; 久保山勉
育種学研究, 2011年
2011, 2011

mRNAの網羅的塩基配列解読によるアサガオSNPマーカーの作出　　　　　　　　　　　　　　　
リートン1; 福岡浩之2; 星野敦3; 飯田滋3; 4; 仁田坂英二5; ○久保山勉1; (1.茨大農、2.農研機構・野菜茶研、3.基生研、4.静岡県立大生活健康科学研究科・薬学研究科、5.九大院理)
日本育種学会第118回講演会, 2010年09月, 日本育種学会

SSRマーカーを用いた日本および中国ラッカセイ品種の識別　　　　　　　　　　　　　　　
○内藤嘉磯1・鈴木茂2・長谷川誠2・桑田主税2・津金胤昭2・渡邉学2・室田有里2・久保山勉3・遠山和宏3・磯部祥子4・佐藤修正4・白澤健太4・笹本茂美4・田畑哲之4(1. 三菱化学メディエンス(株)2. 千葉県農林総合研究センター3. 茨大農4. かずさDNA研)
日本育種学会第118回講演会, 2010年09月, 日本育種学会

Epistatic interaction between an allele of a bacterial blight-resistance gene Xa1 and an allele of a putative transcriptional repressor HWC1 causes hybrid weakness of rice　　　　　　　　　　　　　　　
Tsutomu Kuboyama
国際シロイヌナズナ会議, 2010年06月07日

Study of the causal amino-acid substitutions in HWC2 for a hybrid weakness in rice　　　　　　　　　　　　　　　
Okiyama, Y; N. Katsunuma; K. Ichitani; A. Miyao; H. Hirochika; N. Watanabe; T. Kuboyam
国際シロイヌナズナ会議, 2010年06月, International Coference of Arabidopsis Research

HWC2におけるイネ雑種弱勢誘導原因変異の探索　　　　　　　　　　　　　　　
○沖山友哉1、勝沼法朗1、一谷勝之2、宮尾安藝雄3、廣近洋彦3、渡部信義1、久保山勉1; (1. 茨大農、2. 鹿児島大農、3. 生物研)
日本育種学会第117回講演会, 2010年03月, 日本育種学会

H587を生じる塩基置換がHWC1を雑種弱勢原因遺伝子にする　　　　　　　　　　　　　　　
○勝沼法朗1、沖山友哉1、一谷勝之2、渡部信義1、久保山勉1 (1. 茨大農、2. 鹿児島大農、3. STAFF研、4. 生物研)
日本育種学会第117回講演会, 2010年03月, 日本育種学会

Cucumis属野生種とメロン(C.melo)の種間交雑における花粉管伸長に対する温度の効果　　　　　　　　　　　　　　　
○松本雄一1; 2・宮城慎1・渡部信義2; 3・久保山勉2; 3 (1.茨城農総セ生工研,2.農工大院連農,3.茨城大農)
日本育種学会第117回講演会, 2010年03月, 日本育種学会

ラッカセイにおけるSSRマーカーを用いた連鎖地図作成　　　　　　　　　　　　　　　
○内藤嘉磯1・鈴木茂2・長谷川誠2・渡邉学2・久保山勉3・遠山和宏3・磯部祥子4・佐藤修正4・白澤健太4・笹本茂美4・田畑哲之4(1. 三菱化学メディエンス株2. 千葉県農林総合研究センター3. 茨城大農4. かずさDNA研究所)"
日本育種学会第117回講演会, 2010年03月, 日本育種学会

一粒系コムギの現代化に関する研究　　　　　　　　　　　　　　　
○渡部信義・アノワルルハック・喜多いずみ・小菅一真・久保山勉(茨城大農)
日本育種学会第117回講演会, 2010年03月, 日本育種学会

Genetic mapping of gibberellic acid-sensitive and -insensitive reduced height genes in durum wheat　　　　　　　　　　　　　　　
○A. Haque1; 2・渡部信義1; 2・久保山勉1; 2(1. 農工大院連農2. 茨大農)
日本育種学会第117回講演会, 2010年03月, 日本育種学会

ペチュニアSSRマーカーの開発と連鎖地図の作成　　　　　　　　　　　　　　　
○大高麻美1・河合智香1・小林沙織1・遠山宏和1・安藤敏夫2・渡部信義1・久保山勉1(1. 茨城大学農学部2. 千葉大学園芸学部)"
日本育種学会第116回講演会, 2009年09月, 日本育種学会

ラッカセイ栽培種におけるEST由来SSRマーカーの開発　　　　　　　　　　　　　　　
○内藤嘉磯・鈴木茂・長谷川誠・渡邉学・久保山勉・磯部祥子・佐藤修正・白澤健太・笹本茂美・田畑哲之
日本育種学会第116回講演会, 2009年09月, 日本育種学会

イネ雑種弱勢原因遺伝子HWC2の相補性検定とTos17 挿入を用いた解析　　　　　　　　　　　　　　　
○沖山友哉1、一谷勝之2、勝沼法朗1、金森裕之3、呉健忠4、松本隆4、宮尾安藝雄4、廣近洋彦4、渡部信義1、久保山勉1 (1. 茨大農、2. 鹿児島大農、3. STAFF研、4. 生物研)
日本育種学会第116回講演会, 2009年09月, 日本育種学会

Hwc1、Hwc2によって生じるイネ雑種弱勢における細胞死　　　　　　　　　　　　　　　
○沖山友哉、一谷勝之、藤田雅丈、倉田のり、渡部信義、久保山勉
日本育種学会第115回講演会, 2009年03月, 日本育種学会

マカロニコムギおよび野生二粒系コムギの非帯白性遺伝子の比較マッピング　　　　　　　　　　　　　　　
吉屋康太、渡部信義、久保山勉
日本育種学会第115回講演会, 2009年03月, 日本育種学会

近縁種から導入した青粒性遺伝子(Ba1)をもつマカロニコムギのアイソジーニックライン　　　　　　　　　　　　　　　
渡部信義、小潟絵里子、久保山勉、Peng Z.-S.、Martinek Petr
日本育種学会第115回講演会, 2009年03月, 日本育種学会

メロンとCucumis属野生種の種間交雑における花粉管の挙動　　　　　　　　　　　　　　　
松本雄一、氏家有美、宮城慎、渡部信義、久保山勉
日本育種学会第115回講演会, 2009年03月, 日本育種学会

メロン遺伝資源Cucumis属野生種に対するメロンつる割病レース1,2yの抵抗性評価　　　　　　　　　　　　　　　
松本雄一、小河原孝司、宮本拓也、宮城慎、坂田好輝、渡部信義、久保山勉
園芸学会平成21年度春季大会, 2009年03月, 日本園芸学会

イネ雑種弱勢原因遺伝子Hwc2 の高密度連鎖解析および候補遺伝子　　　　　　　　　　　　　　　
一谷勝之・前田勇介・柳瀬朱・沖山友哉・田; 浦悟・佐藤宗治・金森裕之・呉健忠・松本隆; ・渡部信義・久保山勉
日本育種学会第114回講演会, 2008年10月, 日本育種学会

花粉競争はペチュニアF2分離集団の分離比異常を誘導する　　　　　　　　　　　　　　　
○小林沙織・遠山宏和・渡部信義・久保山勉
日本育種学会第114回講演会, 2008年10月

ラッカセイSSRマーカーの開発　　　　　　　　　　　　　　　
○内藤嘉磯・遠山宏和・井上栄一・渡部信義・久保山勉
日本育種学会第114回講演会, 2008年10月, 日本育種学会

イネ雑種弱勢原因遺伝子Hwc2 領域ORFの相補性検定と発現解析　　　　　　　　　　　　　　　
○沖山友哉・金森裕之・呉健忠・松本隆・一谷勝之・渡部信義・久保山勉
日本育種学会第114回講演会, 2008年10月, 日本育種学会

コムギ属におけるクロリナ突然変異及び長頴遺伝子の比較マッピング　　　　　　　　　　　　　　　
○小菅一真・渡部信義・久保山勉
日本育種学会第114回講演会, 2008年10月, 日本育種学会

コムギ長頴遺伝子の選択的遺伝子浸透の解析　　　　　　　　　　　　　　　
小菅真一、渡部信義、久保山勉
日本育種学会第113回講演会, 2008年03月, 日本育種学会

イネ雑種弱勢原因遺伝子Hwc1の単離　　　　　　　　　　　　　　　
沖山友哉、金森裕之、松本隆、一谷勝之、黒田充宏、渡部信義、安西弘行、久保山勉
日本育種学会第113回講演会, 2008年03月, 日本育種学会

アサガオEST-SSRマーカーの開発　　　　　　　　　　　　　　　
大高麻美1、星野敦2、飯田滋2、福岡浩之3、仁田坂英二4、松藤絵里子1、山口大輔1、久保山勉
日本育種学会第113回講演会, 2008年03月, 日本育種学会

The plants expressing hybrid weakness of the cross between Japanese rice cultivars and Peruvian rice cultivar ‘Jamaica’ have a defect in RAM maintenance　　　　　　　　　　　　　　　
Tsukasa Suzuki; Toshiya Saito; Youya Okiyama; Katsuyuki Ichitani; Wataru Marubashi; Tsutomu Kuboyam
The 5th International Symposium of Rice Functional Genomics, 2007年10月15日

SSRマーカーを利用したラッカセイ栽培種(Arachis hypogea L.)の系統解析　　　　　　　　　　　　　　　
内藤嘉磯; 鈴木茂; 岩田義治; 久保山勉
日本育種学会第112回講演会, 2007年09月23日, 日本育種学会

雑種弱勢原因遺伝子Hwc1候補ORFの発現と雑種弱勢における根端分裂活性のin situ hybridization を用いた解析　　　　　　　　　　　　　　　
鈴木主、一谷勝之、金森裕之、松本隆、渡部信義、久保山勉
日本育種学会第112回講演会, 2007年09月22日, 日本育種学会

Tos17挿入変異を用いたイネ雑種弱勢原因遺伝子Hwc2領域の解析　　　　　　　　　　　　　　　
坂原理紗、黒田充宏、宮尾安藝雄、廣近洋彦、沖山友哉、一谷勝之、久保山勉
日本育種学会第111回講演会, 2007年03月30日, 日本育種学会

イネ雑種弱勢原因遺伝子Hwc2の高密度連鎖解析および品種分化と周辺領域のDNA多型との関係　　　　　　　　　　　　　　　
一谷勝之1,坂本節1,波越啓太1,佐藤宗治1,田浦悟2,金森裕之3; 呉健忠4; 松本隆4; 久保山勉5
日本育種学会第111回講演会, 2007年03月30日, 日本育種学会

四倍性コムギにおける突然変異遺伝子のマッピング　　　　　　　　　　　　　　　
小菅一真、渡部信義、久保山勉、V.M. Melink; V.I. Yanchenko; M.A. Rosova; N.P. Goncharov
日本育種学会第111回講演会, 2007年03月30日, 日本育種学会

日本型イネとペルー品種「ジャマイカ」のF₂にみられた雑種弱勢原因遺伝子の量的効果　　　　　　　　　　　　　　　
齋藤利弥; 揚村京子; 波越啓太; 市田裕之; 林依子; 阿部知子; 西村実; 一谷勝之; 久保山勉
日本育種学会第109回講演会, 2006年09月

イネ雑種弱勢原因遺伝子Hwc1の高密度連鎖解析および候補遺伝子　　　　　　　　　　　　　　　
波越啓太; 田浦悟; 佐藤宗治; 揚村京子; 久保山勉; 一谷勝之
日本育種学会第109回講演会, 2006年09月

ペチュニアSSRマーカーの開発　　　　　　　　　　　　　　　
遠山宏和・井上栄一・久保山勉
日本育種学会第109回講演会, 2006年09月

ペチュニアAn1対立遺伝子間相互作用によって生じる雌性不稔の枝変わりを用いた解析　　　　　　　　　　　　　　　
三木雅史・宮澤美紗子・瀬尾昌子・久保山勉
日本育種学会第109回講演会, 2006年09月

Nicotiana tabacumとSuaveolentes節に属する9種の野生種との種間交雑における雑種致死の発現とその原因となる染色体　　　　　　　　　　　　　　　
手塚孝弘・久保山勉・松田智明、丸橋亘
日本育種学会第109回講演会, 2006年09月

ジャポニカ型イネと品種「Jamaica」の間で見られる雑種弱勢　　　　　　　　　　　　　　　
久保山勉
日本遺伝学会第78回大会, 2006年09月, 日本遺伝学会

Nicotiana tabacumのQ染色体はタバコ属種間雑種（N. tabacum ＸN. debneyi)に認められる雑種致死に伴うプログラム細胞死を引き起こす　　　　　　　　　　　　　　　
手塚孝弘、久保山勉、松田智明、丸橋亘
日本育種学会第108回講演会, 2006年03月

日本型イネとペルー品種「ジャマイカ」のF₂にみられた雑種弱勢原因遺伝子の量的効果　　　　　　　　　　　　　　　
齋藤利弥1・青木美里1・松本雄一1・一谷勝之2・久保山勉
日本育種学会第108回講演会, 2006年03月

Gene-dosage effect of Hwc1, a causal gene of F1 hybrid weakness between Japanese cultivars and a Peruvian cultivar “Jamaica”　　　　　　　　　　　　　　　
Aoki Misato; Toshiya Saito; Yuichi Matsumoto; Katsuyuki Ichitani; Marubashi Wataru; Kuboyama Tsutomu
10th International Congress of SABRAO, 2005年08月22日

Transposon mediated gene disruption of a beta-1,3 glucanase gene expressed in the pollen of petunia　　　　　　　　　　　　　　　
Kumi Kusaka; Tomiko Claudia Otsu; Takeshi Ishimizu; Sumihiro Hase; Tsutomu Kuboyama
10th International Congress of SABRAO, 2005年08月22日

An1遺伝子のトランスポゾンフットプリントが原因で生ずるペチュニア雌性不稔系統の解析　　　　　　　　　　　　　　　
瀬尾昌子・三木雅史・久保山勉
園芸学会平成17年度春季大会, 2005年03月, 日本園芸学会

Rapid differentiation of root cells observed in weak hybrids between Japanese rice cultivar “Nipponbare” and Peruvian rice cultivar “Jamaica”　　　　　　　　　　　　　　　
Saitou Toshiya; Wataru Marubashi; Tsutomu Kuboyama
World Rice Research Conference, 2004年11月07日

キャベツの雑種実生に認められた温度依存性雑種致死　　　　　　　　　　　　　　　
宮崎沙頼・久保山勉・丸橋亘
日本育種学会第106回講演会, 2004年09月21日, 日本育種学会

ペチュニアW138 系統で得られた花粉で発現するβ-1,3グルカナーゼ遺伝子のdTph1挿入突然変異体　　　　　　　　　　　　　　　
日下久美; 大津Claudia 富美子; 石水毅; 長谷純宏; 久保山勉
日本育種学会第106回講演会, 2004年09月21日, 日本育種学会

イネ雑種弱勢遺伝子座Hwc1の同定　　　　　　　　　　　　　　　
一谷勝之,松本雄一,青木美里,丸橋亘,久保山勉
日本育種学会第106回講演会, 2004年09月21日, 日本育種学会

日本型イネとJamaicaの雑種弱勢原因遺伝子Hwc2のポジショナルクローニングに向けて　　　　　　　　　　　　　　　
山内藍・齋藤利弥・松本雄一・林珍仙・一谷勝之・丸橋亘・久保山勉
日本育種学会第105回講演会, 2004年03月31日

交雑不親和性にみられる花粉管伸長阻害現象の研究　　　　　　　　　　　　　　　
久保山勉
日本育種学会第105回講演会, 2004年03月30日, 日本育種学会, ［招待有り］

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