2011年08月, 日本土壌肥料学会奨励賞

2010年07月, RADIOISOTOPES誌論文奨励賞

Search for Expression Marker Genes That Reflect the Physiological Conditions of Blossom End Enlargement Occurrence in Cucumber
Rui Li; Runewa Atarashi; Agung Dian Kharisma; Nur Akbar Arofatullah; Yuki Tashiro; Junjira Satitmunnaithum; Sayuri Tanabata; Kenji Yamane; Tatsuo Sato, Blossom end enlargement (BEE) is a postharvest deformation that may be related to the influx of photosynthetic assimilates before harvest. To elucidate the mechanism by which BEE occurs, expression marker genes that indicate the physiological condition of BEE-symptomatic fruit are necessary. First, we discovered that preharvest treatment with a synthetic cytokinin, N-(2-Chloro-4-pyridyl)-N’-phenylurea (CPPU), promoted fruit growth and suppressed BEE occurrence. This suggests that excessive assimilate influx is not a main cause of BEE occurrence. Subsequently, the expression levels of seven sugar-starvation marker genes, CsSEF1, AS, CsFDI1, CsPID, CsFUL1, CsETR1, and CsERF1B, were compared among symptomatic and asymptomatic fruits, combined with and without CPPU treatment. Only CsSEF1 showed a higher expression level in asymptomatic fruits than in symptomatic fruits, regardless of CPPU treatment. This was then tested using fruits stored via the modified-atmosphere packaging technique, which resulted in a lower occurrence of BEE, and the asymptomatic fruits showed a higher CsSEF1 expression level than symptomatic fruits, regardless of the packaging method. CsSEF1 codes a CCCH-type zinc finger protein, and an increase in the expression of CsSEF1 was correlated with a decrease in the fruit respiration rate. Thus, CsSEF1 may be usable as a BEE expression marker gene., MDPI AG
International Journal of Molecular Sciences, 2024年07月30日, ［査読有り］

Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (Cucumis sativus L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance
Dinar Mindrati Fardhani; Agung Dian Kharisma; Tomoyuki Kobayashi; Nur Akbar Arofatullah; Makoto Yamada; Sayuri Tanabata; Yumi Yokoda; Ani Widiastuti; and Tatsuo Sato, MDPI
Agronomy, 2022年11月29日, ［査読有り］

Effects of Application of Various forms of Nitrogen on the Growth of Soybean Nodules and Roots Related to the Carbon and Nitrogen Metabolism
Takuji Ohyama; Sayuri Tanabata; Norikuni Ohtake; Takashi Sato; Kuni Sueyoshi; Yoshihiko Takahashi; Shinji Ishikawa; Yuki Ono; Natsumi Yamashita; Akinori Saito, Soybean plants require a large amount of nitrogen either from nitrogen fixation in nodules or nitrogen absorption from roots. It is known that nitrate, a major inorganic nitrogen compound in upland soils, represses nodule growth and nitrogen fixation. Rapid and reversible inhibition of nodule growth and nitrogen fixation activity was found in the hydroponically cultivated soybeans after changing the nutrient solution with or without nitrate. Isotope tracer analysis revealed that the major cause of this inhibition depended on the changes in the partitioning of photo-assimilate between nodules and roots and was not directly related to the transported N compounds. Transcriptome and metabolome analyses supported that nitrate strongly promotes nitrogen and carbon metabolism in the roots but represses them in the nodules. The application of ammonium, glutamine, or urea also inhibited the nodule growth and nitrogen fixation like nitrate, although the inhibition was lower than that of nitrate. The degree of inhibition was related to the decrease in carbon isotope partitioning into the nodules, rather than the import of nitrogen isotope to nodules. Urea was detected in xylem sap and all parts of soybean, and some urea might be originated from ureide degradation., IntechOpen
Soybean - Recent Advances in Research and Applications, 2022年09月28日

Cultivation Conditions Affect the Occurrence of Blossom-end Enlargement in Cucumber
Junjira Satitmunnaithum; Hikaru Muroi; Rina Ito; Yuki Tashiro; Antonius Febri Hendratmo; Sayuri Tanabata and Tatsuo Sato, The Japanese Society for Horticultural Science
The Horticulture Journal, 2022年08月02日, ［査読有り］

Microbial population size and strawberry fruit firmness after drop shock-induced mechanical damage
Junjira Satitmunnaithum; Hiroaki Kitazawa; Nur Akbar Arofatullah; Ani Widiastuti; Agung Dian Kharisma; Kenji Yamane; Sayuri Tanabata; Tatsuo Sato, Elsevier
Postharvest Biology and Technology, 2022年06月14日, ［査読有り］

Regulation of defense responses via heat shock transcription factors in Cucumis sativus L. against Botrytis cinerea
Kharisma A.D.; Arofatullah N.A.; Yamane K.; Tanabata S. and Sato T., Springer
Journal of General Plant Pathology, 2021年11月12日, ［査読有り］

Case Study on the Use of the Leaf-Count Method for Drip Fertigation in Outdoor Cucumber Cultivation in Reconstructed Fields Devastated by a Tsunami
Tashiro Y.; Sato S.; Satitmunnaithum J.; Kinouchi H.; Li J. and Tanabata S., MDPI
Agriculture, 2021年07月11日, ［査読有り］

Application of Nitrate, Ammonium, or Urea Changes the Concentrations of Ureides, Urea, Amino Acids and Other Metabolites in Xylem Sap and in the Organs of Soybean Plants (Glycine max (L.) Merr.)
Yuki Ono; Masashige Fukasawa; Kuni Sueyoshi; Norikuni Ohtake; Takashi Sato; Sayuri Tanabata; Ryo Toyota; Kyoko Higuchi; Akihiro Saito and Takuji Ohyama, Soybean (Glycine max (L.) Merr.) plants form root nodules and fix atmospheric dinitrogen, while also utilizing the combined nitrogen absorbed from roots. In this study, nodulated soybean plants were supplied with 5 mM N nitrate, ammonium, or urea for 3 days, and the changes in metabolite concentrations in the xylem sap and each organ were analyzed. The ureide concentration in the xylem sap was the highest in the control plants that were supplied with an N-free nutrient solution, but nitrate and asparagine were the principal compounds in the xylem sap with nitrate treatment. The metabolite concentrations in both the xylem sap and each organ were similar between the ammonium and urea treatments. Considerable amounts of urea were present in the xylem sap and all the organs among all the treatments. Positive correlations were observed between the ureides and urea concentrations in the xylem sap as well as in the roots and leaves, although no correlations were observed between the urea and arginine concentrations, suggesting that urea may have originated from ureide degradation in soybean plants, possibly in the roots. This is the first finding of the possibility of ureide degradation to urea in the underground organs of soybean plants., MDPI
International Journal of Molecular Sciences, 2021年04月, ［査読有り］

Hot Water Spray Over Strawberry Plants Effectively Controls the Occurrence of Strawberry Powdery Mildew in Everbearing Strawberry Production
Dinar Mindrati FARDHANI; Yuuki OHTA; Nur Akbar AROFATULLAH; Agung Dian KHARISMA; Junjira SATITMUNNAITHUM; Sayuri TANABATA; Isao OGIWARA; Ani WIDIASTUTI and Tatsuo SATO, Japanese Society of Farm Work Research
農作業研究, 2021年03月, ［査読有り］

Positron-emitting Tracer Imaging System(PETIS)法を用いたトマト果実への光合成産物の転流に果実直下の側枝葉が及ぼす影響の評価
塚本崇志、石井里美、七夕小百合、鈴井伸郎 、 河地有木、藤巻 秀、草川知行, 園芸学会
園芸学研究, 2020年07月, ［査読有り］

Prevention of powdery mildew disease in tomato nursery by improved hot water spraying device　　　　　　　　　　　　　　　
Nur Akbar Arofatullah; Ani Widiastuti; Yufita Dwi Chinta; Tomoyuki Kobayashi; Sayuri Tanabata; Tatsuo Sato
農作業研究, 2019年06月, ［査読有り］

Effects of Different Chemical Forms of Nitrogen on the Quick and Reversible Inhibition of Soybean Nodule Growth and Nitrogen Fixation Activity
Natsumi Yamashita†; Sayuri Tanabata†; Norikuni Ohtake; Kuni Sueyoshi; Takashi Sato; Kyoko Higuchi; Akihiro Saito; Takuji Ohyama, 筆頭著者, It has been reported that supply of nitrate to culture solution rapidly and reversibly inhibits nodule growth and nitrogen fixation activity of soybean. In this study, the effects of ammonium, urea, or glutamine on nodule growth and nitrogen fixation activity are compared with that for nitrate. Soybean plants were cultivated with a nitrogen-free nutrient solution, then 1 mM-N of nitrate, ammonium, glutamine, or urea were supplied from 12 DAP until 17 DAP. Repression of nodule growth and nitrogen fixation activity at 17 DAP were observed by ammonium, urea, and glutamine like nitrate, although the inhibitory effects were milder than nitrate. The removal of nitrogen from the culture solutions after nitrogen treatments resulted in a recovery of the nodule growth. It was found that the glutamine treatment followed by N-free cultivation gave highest nitrogen fixation activity about two times of the control. Tracer experiments with 15N and 13C were performed to evaluate the translocation of N and C to the different tissues. Culture solutions containing a 15N-labeled nitrogen source were supplied from 21 DAP, and the whole shoots were exposed to 13CO2 for 60 min on 23 DAP, and plants were harvested on 24 DAP. The percentage distribution of 15N in nodules was highest for ammonium (1.4%) followed by glutamine (0.78%), urea (0.32%) and nitrate (0.25%). The percentage distribution of 13C in the nodules was highest for the control (11.5%) followed by urea (5.8%), glutamine (2.6%), ammonium (2.3%), and nitrate (2.3%). The inhibitory effects of nitrogen compounds appeared to be related to a decrease in photoassimilate partitioning in the nodules, rather than 15N transport into the nodules. The free amino acid concentrations after nitrogen treatments were increased in the nodules and leaves by nitrate, in the roots by ammonium, in the stems by urea, and the roots, stems, and leaves by glutamine treatment. The concentrations of asparagine, aspartate, and glutamine were increased after nitrogen treatments. By the long-term supply of nitrogen for 2-weeks, nitrate significantly increased the lateral roots and leaf growth. The long-term supply of urea and glutamine also promoted the lateral roots and leaf growth, but ammonium suppressed them.
Frontiers in Plant Science, 2019年02月19日, ［査読有り］

Effect of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate on the Deep Placement of Nitrogen Fertilizers for Soybean Cultivation
Soshi Hatano; Yoichi Fujita; Yoshifumi Nagumo; Norikuni Ohtake; Kuni Sueyoshi; Yoshihiko Takahashi; Takashi Sato; Sayuri Tanabata; Kyoko Higuchi; Akihiro Saito; Takuji Ohyama
International Journal of Agronomy, 2019年02月03日, ［査読有り］

Heat Shock-Induced Resistance against Pseudomonas syringae pv. tomato (Okabe) Young et al. via Heat Shock Transcription Factors in Tomato
Nur Akbar Arofatullah; Morifumi Hasegawa; Sayuri Tanabata; Isao Ogiwara; Tatsuo Sato, *Abstract: We investigated the role of heat shock transcription factors (Hsfs) during induction of defense response by heat-shock treatment (HST) in tomato. Leaf disease symptoms were significantly reduced at 12 and 24 h after HST, consistent with upregulation of pathogenesis-related (PR) genes PR1a2 and PR1b1 peaking at 24 h after treatment. These genes were upregulated at the treatment application site, but not in untreated leaves. In contrast to HST, inoculation of the first leaf induced systemic upregulation of acidic PR genes in uninoculated second leaves. Furthermore, heat shock element motifs were found in upstream regions of PR1a2, PR1b1, Chitinase 3, Chitinase 9, Glucanase A, and Glucanase B genes. Upregulation of HsfA2 and HsfB1 peaked at 6 h after HST, 6 h earlier than salicylic acid accumulation. Foliar spray of heat shock protein 90 (Hsp90) inhibitor geldanamycin (GDA) induced PR gene expression comparable to that after HST. PR gene expression and defense response against Pseudomonas syringae pv. tomato (Pst) decreased when combining HST with Hsfs inhibitor KRIBB11. The Hsfs and PR gene expression induced by heat or GDA, together with the suppression of heat shock-induced resistance (HSIR) against Pst by KRIBB11, suggested a direct contribution of Hsfs to HSIR regulation in tomato., MDPI AG
Agronomy, 2018年12月20日, ［査読有り］

Transcriptome and Metabolome Analyses Reveal That Nitrate Strongly Promotes Nitrogen and Carbon Metabolism in Soybean Roots, but Tends to Repress It in Nodules
Ishikawa S.; Ono Y.; Ohtake N.; Sueyoshi K.; Tanabata S. and Ohyama T., Leguminous plants form root nodules with rhizobia that fix atmospheric dinitrogen (N2) for the nitrogen (N) nutrient. Combined nitrogen sources, particular nitrate, severely repress nodule growth and nitrogen fixation activity in soybeans (Glycine max [L.] Merr.). A microarray-based transcriptome analysis and the metabolome analysis were carried out for the roots and nodules of hydroponically grown soybean plants treated with 5 mM of nitrate for 24 h and compared with control without nitrate. Gene expression ratios of nitrate vs. the control were highly enhanced for those probesets related to nitrate transport and assimilation and carbon metabolism in the roots, but much less so in the nodules, except for the nitrate transport and asparagine synthetase. From the metabolome analysis, the concentration ratios of metabolites for the nitrate treatment vs. the control indicated that most of the amino acids, phosphorous-compounds and organic acids in roots were increased about twofold in the roots, whereas in the nodules most of the concentrations of the amino acids, P-compounds and organic acids were decreased while asparagine increased exceptionally. These results may support the hypothesis that nitrate primarily promotes nitrogen and carbon metabolism in the roots, but mainly represses this metabolism in the nodules., MDPI AG
Plants, 2018年04月12日, ［査読有り］

チンゲンサイ栽培による水熱分解液肥の作物生育に及ぼす効果と阻害の特性評価
大池新二郎、七夕小百合、鈴木千夏、小沢聖、藤原俊六郎, 生態工学会
Eco-Engineering, 2017年02月, ［査読有り］

Application of glutathione and dithiothreitol to oil seed rape (Brassica napus L.) roots affects cadmium distribution in roots and inhibits Cd translocation to shoots
Shin-ichi Nakamura; Nobuo Suzui; Sayuri Ito-Tanabata; Satomi Ishii; Naoki Kawachi; Hiroki Rai; Hiroyuki Hattori; Shu Fujimaki, Previously we have shown that site-specific application of glutathione (GSH) to roots inhibits cadmium (Cd) translocation from roots to shoots. In this study, we investigated the effects of chemical compounds containing sulfur, such as dithiothreitol (DTT), potassium sulfate (K2SO4) and a GSH synthesis inhibitor, buthionine sulfoximine (BSO), on the behavior of Cd in oilseed rape plants (Brassica napus L.). We found that DTT, just like GSH, also inhibited Cd translocation from roots to shoots significantly. However, the inhibitory effects of Cd behaviors differed between GSH and DTT. Cd efflux from root cells was activated by GSH and inactivated by DTT. Positron imaging experiments revealed that there are minor differences in the effects of GSH and DTT on Cd adsorption to the surface of roots. These results provide insights into the function of GSH involved in the inhibitory effects of Cd translocation from roots to shoots., Taylor and Francis Ltd.
Soil Science and Plant Nutrition, 2016年07月03日, ［査読有り］

Effect of Nitrate on Nodule and Root Growth of Soybean (Glycine max (L.) Merr.)
Saito A.†; Tanabata S.†; Tanabata T.; Tajima S.; Ueno M.; Ishikawa S.; Ohtake N.; Sueyoshi K. and Ohyama T., The application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation. In the present study, we measured the effects of supplying 5 mM nitrate on the growth of nodules, primary root, and lateral roots under light at 28 degrees C or dark at 18 degrees C conditions. Photographs of the nodulated roots were periodically taken by a digital camera at 1-h intervals, and the size of the nodules was measured with newly developed computer software. Nodule growth was depressed approximately 7 h after the addition of nitrate under light conditions. The nodule growth rate under dark conditions was almost half that under light conditions, and nodule growth was further suppressed by the addition of 5 mM nitrate. Similar results were observed for the extending growth rate of the primary root as those for nodule growth supplied with 5 mM nitrate under light/dark conditions. In contrast, the growth of lateral roots was promoted by the addition of 5 mM nitrate. The 2D-PAGE profiles of nodule protein showed similar patterns between the 0 and 5 mM nitrate treatments, which suggested that metabolic integrity may be maintained with the 5 mM nitrate treatment. Further studies are required to confirm whether light or temperature condition may give the primary effect on the growth of nodules and roots., MDPI AG
International Journal of Molecular Sciences, 2014年03月, ［査読有り］

Temperature affects N and C assimilation and translocation in Curcuma alismatifolia Gagnep
T. Khuankaew; S. Tanabata; M. Yamamoto; S. Ishikawa; K. Tsutsumi; N. Ohtake; K. Sueyoshi; T. Ohyama; T. Sato; C. Anuwong; S. Ruamrungsri, Curcuma alismatifolia has become popular as an ornamental plant because of its colourful, long-lasting, and repeated growth of inflorescences. The effects of temperature on N and C assimilation and transport during the initial flowering stage were investigated using 15N and 13C. Treatments at 15°C, 25°C, or 35°C had no significant effect on total 15N absorption by the plants. However, the distribution of 15N was affected by temperature. The concentration of 15N in new rhizomes and leaves was lower at 15°C than at 25°C, although the concentrations of 15N in old rhizomes and old storage roots were higher. The concentration of 15N in roots and leaves was lower at 35°C than at 25°C.Total plant 13C concentrations were highest at 25°C, followed by 15°C, and lowest at 35°C. The concentration of 13C in new rhizomes was the highest, followed by leaves and inflorescences at 25°C. The concentrations and distribution of 13C in new rhizomes and in new storage roots were highest at 25°C. These results indicate that temperature regulates the assimilation and transport of N and C, with the optimum temperature being between 25° - 35°C., Headley Brothers Ltd
Journal of Horticultural Science and Biotechnology, 2014年

ダイズ根粒成長解析のためのコンピュータ画像計測方法
七夕小百合; 七夕高也; 齋藤明徳; 田嶋誠也; 渡辺詩織; 石川慶祐; 大竹憲邦; 末吉邦; 大山卓爾
日本土壌肥料学雑誌, 2014年, ［査読有り］

A kinetic analysis of cadmium accumulation in a Cd hyper-accumulator fern, Athyrium yokoscense and tobacco plants.
Yoshihara T.; Suzui N.; Ishii S.; Kitazaki M.; Yamazaki H.; Kitazaki K.; Kawachi N.; Yin Y.G.; Ito-Tanabata S.; Hashida S.; Shoji K.; Shimada H.; Goto F. and Fujimaki S., Cadmium (Cd) accumulations in a Cd hyper-accumulator fern, Athyrium yokoscense (Ay), and tobacco, Nicotiana tabacum (Nt), were kinetically analysed using the positron-emitting tracer imaging system under two medium conditions (basal and no-nutrient). In Ay, maximumly 50% and 15% of the total Cd accumulated in the distal roots and the shoots under the basal condition, respectively. Interestingly, a portion of the Cd in the distal roots returned to the medium. In comparison with Ay, a little fewer Cd accumulations in the distal roots and clearly higher Cd migration to the shoots were observed in Nt under the basal condition (maximumly 40% and 70% of the total Cd, respectively). The no-nutrient condition down-regulated the Cd migration in both species, although the regulation was highly stricter in Ay than in Nt (almost no migration in Ay and around 20% migration in Nt). In addition, the present work enabled to estimate physical and physiological Cd accumulation capacities in the distal roots, and demonstrated condition-dependent changes especially in Ay. These results clearly suggested occurrences of species-/condition-specific regulations in each observed parts. It is probable that integration of these properties govern the specific Cd tolerance/accumulation in Ay and Nt., WILEY-BLACKWELL
Plant, Cell and Environment, 2014年, ［査読有り］

Application of glutathione to roots selectively inhibits cadmium transport from roots to shoots in oilseed rape.
Nakamura S.; Suzui N.; Nagasaka T.; Komatsu F.; Ishioka S.N.; Ito-Tanabata S.; Kawachi N.; Rai H.; Hattori H.; Chino M. and Fujimaki S., Glutathione is a tripeptide involved in various aspects of plant metabolism. This study investigated the effects of the reduced form of glutathione (GSH) applied to specific organs (source leaves, sink leaves, and roots) on cadmium (Cd) distribution and behaviour in the roots of oilseed rape plants (Brassica napus) cultured hydroponically. The translocation ratio of Cd from roots to shoots was significantly lower in plants that had root treatment of GSH than in control plants. GSH applied to roots reduced the Cd concentration in the symplast sap of root cells and inhibited root-to-shoot Cd translocation via xylem vessels significantly. GSH applied to roots also activated Cd efflux from root cells to the hydroponic solution. Inhibition of root-to-shoot translocation of Cd was visualized, and the activation of Cd efflux from root cells was also shown by using a positron-emitting tracer imaging system (PETIS). This study investigated a similar inhibitory effect on root-to-shoot translocation of Cd by the oxidized form of glutathione, GSSG. Inhibition of Cd accumulation by GSH was abolished by a low-temperature treatment. Root cells of plants exposed to GSH in the root zone had less Cd available for xylem loading by actively excluding Cd from the roots. Consequently, root-to-shoot translocation of Cd was suppressed and Cd accumulation in the shoot decreased., OXFORD UNIV PRESS
Journal of Experimental Botany, 2013年, ［査読有り］

Real-time whole-plant imaging of C-11 translocation using positron-emitting tracer imaging system
Naoki Kawachi; Nobuo Suzui; Satomi Ishii; Sayuri Ito; Noriko S. Ishioka; Haruaki Yamazaki; Aya Hatano-Iwasaki; Ken'ichi Ogawa; Shu Fujimaki, Whole-plant imaging for studying the complete carbon kinetics involved in photosynthesis and subsequent photoassimilate translocation and unloading was achieved using a positron-emitting tracer imaging system (PETIS) in combination with (CO2)-C-11 gas tracer. In an experiment with a soybean (Glycine max cultivar Jack) plant, it was confirmed that the dynamic PETIS image data obtained followed the conservation law for total carbon. Thus, the proposed PETIS technique is a feasible noninvasive and quantitative solution to study the carbon dynamics over an entire plant in environmental and agricultural studies. (C) 2010 Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2011年08月, ［査読有り］

Nitrogen and carbon translocation and distribution in curcuma alismatifolia gagnep. by labelling experiment with 15N and 13C
T. Khuankaew; S. Ito; N. Ohtake; K. Sueyoshi; T. Ohyama; T. Sato; S. Ruamrungsri, Curcuma alismatifolia Gagnep. rhizomes were planted on 15 May 2007. At 10 weeks after planting, at the termination of the new leaf formation stage, the plants were supplied with 15N-labeled culture solution (3.81 mM NH4+ + 8.96 mM NO3-: 10.6 atom% 15N) twice at 3 and 1 days before 13CO2 feeding. Then 13C-labeled 13CO2 (99 atom% 13C) was exposed to the shoot. The plants were harvested at four different stages of growth. The 15N and 13C abundance in each part were determined to investigate the characteristics of N and C translocation and distribution in curcuma. The total dry weights of the plant increased continuously during the termination of the new leaf formation stage (stage 1), the flowering stage (stage 2) and the rhizome storage period (stage 3) and especially rapidly increased during stage 2 to stage 3. The DW was relatively constant thereafter until the start of the dormancy period (stage 4). The total N content of the plant rapidly increased after stage 2 until stage 4. At stage 1, the total N was distributed mostly to the old storage roots, leaves and old rhizome just as the dry weight. At stage 2, the dry weights of new organs were mostly increased as well as the N content. At stage 3 and stage 4, the total N content of the new storage organs (new rhizomes and new storage roots) was highly increased, particularly in the new rhizome. The old rhizome and the old storage roots gave very low 15N and 13C in abundance. At stage 1, leaves are the main N sink and about 5.17 mg labeled N (LN) assimilated to this organ. In this stage, translocation of the newly assimilated N to the new rhizome already started. At stage 2, the distribution of LN was increased in flower and flower stalk but it was less than in the leaves that are also the main sink of N organ. At stage 3 the distribution of LN to the aboveground part organs (leaves, flowers and flower stalks) was decreased. This contrasted to the underground part organs (new rhizome, new storage roots and fibrous roots) where LN increased especially in the new rhizome. At stage 4, the 15N was mostly distributed to the storage organ (new rhizome and new storage roots) about 8.85 mg LN and 3.59 mg LN, respectively., International Society for Horticultural Science
Acta Horticulturae, 2011年01月31日

Real-time imaging and analysis of differences in cadmium dynamics in rice cultivars (Oryza sativa) using positron-emitting ¹⁰⁷Cd tracer.
Ishikawa S.; Suzui N.; Ito-Tanabata S.; Ishii S.; Igura M.; Abe T.; Kuramata M.; Kawachi N. and Fujimaki S., Background: Rice is a major source of dietary intake of cadmium (Cd) for populations that consume rice as a staple food. Understanding how Cd is transported into grains through the whole plant body is necessary for reducing rice Cd concentrations to the lowest levels possible, to reduce the associated health risks. In this study, we have visualized and quantitatively analysed the real-time Cd dynamics from roots to grains in typical rice cultivars that differed in grain Cd concentrations. We used positron-emitting(107)Cd tracer and an innovative imaging technique, the positron-emitting tracer imaging system (PETIS). In particular, a new method for direct and real-time visualization of the Cd uptake by the roots in the culture was first realized in this work.
Results: Imaging and quantitative analyses revealed the different patterns in time-varying curves of Cd amounts in the roots of rice cultivars tested. Three low-Cd accumulating cultivars (japonica type) showed rapid saturation curves, whereas three high-Cd accumulating cultivars (indica type) were characterized by curves with a peak within 30 min after(107)Cd supplementation, and a subsequent steep decrease resulting in maintenance of lower Cd concentrations in their roots. This difference in Cd dynamics may be attributable to OsHMA3 transporter protein, which was recently shown to be involved in Cd storage in root vacuoles and not functional in the high-Cd accumulating cultivars. Moreover, the PETIS analyses revealed that the high-Cd accumulating cultivars were characterized by rapid and abundant Cd transfer to the shoots from the roots, a faster transport velocity of Cd to the panicles, and Cd accumulation at high levels in their panicles, passing through the nodal portions of the stems where the highest Cd intensities were observed.
Conclusions: This is the first successful visualization and quantification of the differences in whole-body Cd transport from the roots to the grains of intact plants within rice cultivars that differ in grain Cd concentrations, by using PETIS, a real-time imaging method., BIOMED CENTRAL LTD
BMC Plant Biology, 2011年, ［査読有り］

¹¹CO₂と植物用ポジトロンイメージング装置を用いたダイズ植物の根粒に対する光合成産物移行のリアルタイム解析.
伊藤小百合、鈴井伸郎、河地有木、石井里美、石岡典子、藤巻秀, 日本アイソトープ協会
RADIOISOTOPES, 2010年, ［査読有り］

Assimilation and translocation of nitrogen and carbon in Curcuma alismatifolia Gagnep.
Khuankaew T.; Ruamrangsri S.; Ito S.; Sato T.; Ohtake N.; Sueyoshi K. and Ohyama T., Curcuma or Siam tulip (Curcuma alismatifolia Gagnep.) is an ornamental flowering plant with two underground storage organs, rhizomes and storage roots. Characteristics of N and C assimilation and transport in curcuma were investigated. The plants were treated with (15)NH(4)(+) + (15)NO(3)(-)) and (13)CO(2) at 10, 13 or 21 weeks after planting. Plants were sampled at several stages up to 32 weeks. The C stored in old storage roots was used rapidly during the first 10 weeks; after which N stored in old rhizomes and old storage roots were used. The daily gain in C depending on photosynthesis was remarkably high between 10 and 21 weeks. However, the daily gain in N was relatively constant throughout the growth period. The (15)N absorbed at 10 weeks was initially accumulated in leaves and roots, but some was transported to flowering organs at 13 weeks. At harvest, 41% of (15)N was recovered in new rhizomes and 17% in new storage roots. After (13)CO(2) exposure at 10 and 13 weeks, the distribution of (13)C among organs was relatively constant in subsequent stages. When given (13)CO(2) at 21 weeks, a large amount of labelled C was recovered in new storage roots and new rhizomes at harvest. Both new rhizomes and new storage roots stored N and C, however, rhizomes played a more important role in supplying N, while storage roots provided C., WILEY-BLACKWELL
Plant Biology, 2010年, ［査読有り］

Tracing cadmium from culture to spikelet: non-invasive imaging and quantitative characterization of absorption, transport and accumulation of cadmium in an intact rice plant.
Fujimaki S.; Suzui N.; Ishioka S.N.; Kawachi N.; Ito S.; Chino M. and Nakamura S., We characterized the absorption and short-term translocation of cadmium (Cd) in rice (Oryza sativa 'Nipponbare') quantitatively using serial images observed with a positron-emitting tracer imaging system. We fed a positron-emitting 107 Cd (half-life of 6.5 h) tracer to the hydroponic culture solution and noninvasively obtained serial images of Cd distribution in intact rice plants at the vegetative stage and at the grain-filling stage every 4 min for 36 h. The rates of absorption of Cd by the root were proportional to Cd concentrations in the culture solution within the tested range of 0.05 to 100 nM. It was estimated that the radial transport from the culture to the xylem in the root tissue was completed in less than 10 min. Cd moved up through the shoot organs with velocities of a few centimeters per hour at both stages, which was obviously slower than the bulk flow in the xylem. Finally, Cd arrived at the panicles 7 h after feeding and accumulated there constantly, although no Cd was observed in the leaf blades within the initial 36 h. The nodes exhibited the most intensive Cd accumulation in the shoot at both stages, and Cd transport from the basal nodes to crown root tips was observed at the vegetative stage. We conclude that the nodes are the central organ where xylem-to-phloem transfer takes place and play a pivotal role in the half-day travel of Cd from the soil to the grains at the grain-filling stage., AMER SOC PLANT BIOLOGISTS
Plant Physiology, 2010年, ［査読有り］

Molecular Imaging for Plant Physiology: Imaging of Carbon Trans location to Sink Organs
N. Kawachi; N. Suzui; S. Ishii; S. Ito; N. S. Ishioka; K. Kikuchi; T. Tsukamoto; T. Kusakawa; F. Fujimaki, Radionuclide-based imaging technologies have provided exciting new opportunities to life science researchers. However, success in the field of plant science is limited because most methods for studying plants are invasive and require statistical analysis and a large number of test plants. To clarify the mechanism of the growth and development of the agricultural produces to be harvested, we performed imaging experiments of sugar translocation to the sink organ of fruit. A leaf near the target fruits (eggplant and tomato) was exposed to carbon-11-labeled carbon dioxide, and the translocations of carbon-11-labeled photoassimilate into fruits were assessed by images obtained using the positron-emitting tracer imaging system and small animal positron emission tomography system. Serial images thus obtained showed gradually increasing C-11 activity and its nonuniform distribution in the fruit. These experimental methods will be useful in not only investigating plant physiology, such as mechanisms underlying fruit growth, but also solving certain environmental and food problems., IEEE
2009 IEEE NUCLEAR SCIENCE SYMPOSIUM CONFERENCE RECORD, VOLS 1-5, 2009年, ［査読有り］

Carbon and nitrogen transport during grain filling in rice under high-temperature conditions.
Ito S.; Hara T.; Kawanami Y.; Watanabe T.; Thiraporn K.; Ohtake N.; Sueyoshi K.; Mitsui T.; Fukuyama T.; Takahashi Y.; Sato T.; Sato A. and Ohyama T., Rice (Oryza sativa cv. Koshihikari) seedlings were grown in a sandy dune soil in pots with a basal dressing of N (0.5 g N), P and K. Two N treatments were applied, a +N treatment in which a top dressing of (15)N-labeled 0.5 g N was supplied on July 20 and a -N treatment in which no additional fertilizer was supplied. During the grain-filling stage from August 6 to 13, plants were subjected to one of three temperature treatments; controlled low temperature, LT (day/night 28/23 degrees C), controlled high temperature, HT (35/30 degrees C) and uncontrolled glasshouse temperature, UT (day/night averages, 38/26 degrees C). All plants were then exposed to (13)CO(2) for 1 h on August 11 in a growth chamber at 25 degrees C. On August 13, all plants were harvested and the (13)C and (15)N abundances and starch and sugar concentrations in the ears, shoots and roots were determined. The (13)C content of the ear was lower in UT than in LT irrespective of the +N or -N treatment. The translocation of (15)N to the ears was also slightly depressed in UT compared with LT. Under high-temperature conditions (HT and UT), the starch content per plant was reduced for -N, but for +N, it was not significantly different among the temperature treatments. A high accumulation of sucrose was observed in all plant parts under UT conditions. It is suggested that extreme high day temperatures during the grain-filling period may reduce starch synthesis in the grains and, especially so under N-deficient conditions. High temperatures also induce an accumulation of sucrose and a decrease in carbon and nitrogen transport from the shoots to the ears via the phloem., WILEY-BLACKWELL PUBLISHING, INC
Journal of Agronomy and Crop Science, 2009年, ［査読有り］

Real-time imaging of nitrogen fixation in an intact soybean plant with nodules using ¹³N-labeled nitrogen gas.
Ishii S.; Suzui N.; Ito S.; Ishioka S. N.; Kawachi N.; Ohtake N.; Ohyama T. and Fujimaki S., Real-time images of nitrogen fixation in an intact nodule of hydroponically cultured soybean (Glycine max [L] Merr.) were obtained. In the present study, we developed a rapid method to produce and purify 13N-labeled radioactive nitrogen gas (half life: 9.97 min). 13N was produced from a 16O (p, alpha) 13N nuclear reaction. The target chamber was filled with CO(2) and irradiated for 10 min with protons at an energy of 18.3 MeV and an electric current of 5 mu A, which was delivered from a cyclotron. All CO(2) in the collected gas was absorbed and removed with powdered soda-lime in a syringe and replaced with helium gas. The resulting gas was injected into gas chromatography and separated and a 35 mL fraction, including the peak of [13N]-nitrogen gas, was collected by monitoring the chromatogram. The obtained gas was mixed with 10 mL of O(2) and 5 mL of N(2) and used in the tracer experiment. The tracer gas was fed into the underground part of intact nodulated soybean plants and serial images of the distribution of 13N were obtained non-invasively using a positron-emitting tracer imaging system (PETIS). The rates of nitrogen fixation of the six test plants were estimated to be 0.17 +/- 0.10 mu mol N(2) h-1 from the PETIS image data. The decreasing rates of assimilated nitrogen were also estimated to be 0.012 +/- 0.011 mu mol N(2) h-1. In conclusion, we successfully observed nitrogen fixation in soybean plants with nodules non-invasively and quantitatively using [13N]N(2) and PETIS., WILEY-BLACKWELL PUBLISHING, INC
Soil Science and Plant Nutrition, 2009年, ［査読有り］

MECHANISM OF QUICK AND REVERSIBLE INHIBITION OF SOYBEAN NODULE GROWTH AND NITROGEN FIXATION ACTIVITY BY NITRATE AND ITS METABOLITES
T. Ohyama; A. Yamazaki; N. Yamashita; T. Kimura; S. Ito; N. Ohtake; K. Sueyoshi, SPRINGER
BIOLOGICAL NITROGEN FIXATION: TOWARDS POVERTY ALLEVIATION THROUGH SUSTAINABLE AGRICULTURE, 2008年, ［査読有り］

COMPARISON OF LEAF GROWTH OF HYPERNODULATION SOYBEAN MUTANTS, NOD1-3, NOD2-4 AND NOD3-7, WITH THEIR PARENT CV. WILLIAMS
S. Ito; N. Ohtake; K. Sueyoshi; T. Ohyama, SPRINGER
BIOLOGICAL NITROGEN FIXATION: TOWARDS POVERTY ALLEVIATION THROUGH SUSTAINABLE AGRICULTURE, 2008年, ［査読有り］

The autoregulation of nodulation mechanism is related to leaf development.
Ito S.; Kato T.; Ohtake N.; Sueyoshi K. and Ohyama T., To understand the autoregulation of nodulation (AON) system, in which leguminous plants control the nodule number, we examined the details of the characteristics of hypernodulation soybean mutants NOD1-3 and NOD3-7. A microscopic study showed that NOD1-3 and NOD3-7 produced small-size leaves due to the smaller number of leaf cells, compared with the Williams parent. These phenotypes were not affected by inoculation with bradyrhizobia or nitrate supply. The AON signaling might be related to the control system of leaf cell proliferation. This hypothesis was strongly supported by the finding that activation of AON in wild types by inoculation leads to an increase in the cell number of leaves., OXFORD UNIV PRESS
Plant and Cell Physiology, 2008年01月, ［査読有り］

Characteristics of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, affected by inoculation of bradyrhizobia and nitrate supply.
Ito S.; Ohtake N.; Sueyoshi K. and Ohyama T., The characteristics of plant growth of hypernodulation soybean mutant lines NOD1-3, NOD2-4 and NOD3-7 were compared with their parent cv. Williams. The plants were grown using hydroponics in an illuminated growth chamber with or without seed inoculation of bradyrhizobia, and in the absence or presence of nitrate in the culture solution. When the plants were grown without inoculation, the total dry weight of all mutant lines was not different statistically from Williams, both in the absence and presence of nitrate. When they were grown with inoculation of bradyrhizobia, however, the total dry weight of each mutant line was significantly lower than that of Williams, both in the absence and presence of nitrate. These results indicate that the reduced accumulation of total dry matter of hypernodulation mutant lines compared with the wild type may be a secondary effect passively resulting from the large number of nodules, while the hypernodulation trait is a primary effect of the mutated gene. When the plants were grown with inoculation, the nodule number was decreased by the presence of nitrate in Williams, NOD1-3 and NOD2-4, but not in NOD3-7. NOD3-7 may be the most tolerant to nitrate inhibition of nodulation among the NOD mutant lines. In contrast, leaf growth of NOD3-7 and NOD1-3 was different from the wild type; the expanded leaf was smaller, but the leaf emergence rate was faster compared with Williams under all conditions. This indicates that NOD3-7 and NOD1-3 might decrease the ability for leaf expansion or may have a faster leaf emergence rate., BLACKWELL PUBLISHING
Soil Science and Plant Nutrition, 2007年, ［査読有り］

Effect of deep placement of slow-release fertilizer (Lime nitrogen) applied at different rates on growth, N-2 fixation and yield of soya bean (Glycine max [L.] Merr.)
T. Kaushal; M. Onda; S. Ito; A. Yamazaki; H. Fujikake; N. Ohtake; K. Sueyoshi; Y. Takahashi; T. Ohyama, A new fertilization method with deep placement of slow-release N fertilizers, such as coated urea and lime nitrogen (LN) (calcium cyanamide) at 20 cm depth was found to promote soy bean seed yield. In the present study, the effect of deep placement of LN was investigated on different parameters such as growth, N accumulation, NZ fixation activity and yield of soy bean by applying LN at different rates in the rotated paddy field of Niigata, Japan. In addition to the basal fertilizer, ammonium sulphate (16 kg N ha(-1)), deep placement of LN was conducted by applying various amounts such as 50 kg N ha(-1) (A50), 100 kg N ha(-1) (A100) and 200 kg N ha(-1) (A200) at 20 cm depth in separate plots. A N-15-labelled LN fertilizer was also employed for each of the above treatments to calculate N utilization from LN in separate plots. Soya bean plant growth and N-2 fixation activity were periodically analysed. Both plant growth and N accumulation were found to increase with LN treatment compared with control plants. An increase in N-2 fixation activity was found in the A100 plots. The total seed yield was the highest in the deep placement of LN with A100 (73 g per plant) compared with other treatments. The visual quality of harvested seeds also showed that A100 enhanced the quality of seeds compared with other treatments. Thus, it is suggested that N fertilization management with particular reference to optimum amount of fertilizers is important for maximum growth. N-2 fixation and enhancement of seed yield of soy bean., BLACKWELL PUBLISHING
JOURNAL OF AGRONOMY AND CROP SCIENCE, 2006年12月, ［査読有り］

硝酸イオンによるダイズ根粒の肥大生長と窒素固定活性の阻害機構
大山 卓爾; 伊藤 小百合; 大竹 憲邦; 末吉 邦, Japan Society for Bioscience, Biotechnology, and Agrochemistry
化学と生物, 2006年11月01日

Comparison of the depth of placement of lime nitrogen on growth, N-2 fixation activity, seed yield and quality of soybean (Glycine max (L.) Merr.) plants
Tewari Kaushal; Masaru Onda; Sayuri Ito; Akihiko Yamazaki; Hiroyuki Fujikake; Norikuni Ohtake; Kuni Sueyoshi; Yoshihiko Takahashi; Yoshifumi Nagumo; Tohru Tsuchida; Takuji Ohyama, We have shown in previous reports that a new fertilization method with deep placement of slow-release N fertilizers, such as coated urea and lime nitrogen (calcium cyanamide), at a depth of 20 cm promoted soybean growth and seed yield. In the present study, the effect of deep placement of lime nitrogen (which gave the highest yield in our previous experiment) was compared at various depths in a rotated paddy field at the Niigata Agricultural Research Institute. The objective of the present study was to analyze growth, N-2 fixation activity and seed yield, and to examine visually the quality of soybean seeds cultivated at different depths with the slow-release fertilizer, lime nitrogen. In addition to the conventional basal application of mixed chemical fertilizer (including ammonium sulfate 16 kg N ha(-1)) in 0-10 cm soil layers, deep placement of lime nitrogen was conducted by applying at various depths (10 cm [D10], 15 cm [D15] and 20 cm [D20]) the same amount (100 kg N ha(-1)) in separate plots. N-15-labeled lime nitrogen fertilizers were also used for each of the above treatments to calculate N absorption in relation to fertilization depth. Soybean plant growth was periodically analyzed and the quality of harvested seeds was also visually examined (hereafter referred to as "visual quality"). The total seed yield was highest in the deep placement of lime nitrogen (100 kg N ha(-1)) at D20 (69 g plant(-1)) compared with D15 (59 g plant(-1)), D10 (57 g plant(-1)) and the control (48 g plant(-1)). The visual quality of harvested seeds showed that lime nitrogen (20 cm depth and 100 kg N ha(-1)) enhanced the quality of seeds compared with the other treatments, in which the good quality seeds (hereafter referred to as "good seeds") based on dry weight were 24 g plant(-1) (control), 37 g plant(-1) (D10), 33 g plant(-1) (D15) and 45 g plant(-1) (D20). Thus, it appears that N fertilization management with particular reference to the depth of fertilizer placement is important for maximum yield and enhancement of seed quality of soybean., BLACKWELL PUBLISHING
SOIL SCIENCE AND PLANT NUTRITION, 2006年08月, ［査読有り］

Allocation of photosynthetic products in soybean during the early stages of nodule formation.
Ito S.; Ohtake N.; Sueyoshi K. and Ohyama T., A time-course study examining the current photosynthate allocation of soybean (Glycine max [L.] Merr.) cv. Williams was conducted in relation to nodule initiation. Whole shoots were exposed to (CO2)-C-14 for 120 min and the distribution of radioactivity in each organ was determined. During the early stages of nodule formation (i.e. 4, 6 and 8 days after inoculation) the C-14 distribution to the inoculated roots did not increase when compared with uninoculated control roots. In addition, the C-14 respired by underground parts was similar in both the inoculated and the control roots. Eight days after inoculation, the accumulation of starch and sugar was similar in both inoculated and uninoculated plants. These results indicate that photosynthate allocation for nodule initiation does not increase markedly during the early stages of nodule formation. After the emergence of the nodules, photosynthate allocation to the inoculated roots gradually increased. In addition, the consumption of current photosynthate by the respiration of underground parts increased at day 12 after inoculation, but did not increase at day 8 after inoculation., BLACKWELL PUBLISHING
Soil Science and Plant Nutrition, 2006年, ［査読有り］

Effect of nitrogen supply on nitrogen and carbohydrate constituent accumulation in rhizomes and storage root of Curcuma alismatifolia Gagnep.
Ohtake N.; Ruamrungsri S.; Ito S.; Sueyoshi K.; Ohyama T. and Apavatjrut P., Curcuma (Curcuma alismatifolia cv. Gagnep.), a tropical flowering plant known as "Siam tulip", were cultivated in a pot with vermiculite and supplied with different levels of nitrogen (N). Rhizomes with storage roots were harvested at 215 days after planting. Results indicated that a high level of N supply increased flower numbers and promoted continuous new rhizome formation, but storage root growth was depressed. The N supply to the plants increased the N concentrations both in the rhizomes and in the storage roots. The predominant nitrogenous compounds related to total N increase were proteins in the rhizomes. The N of the insoluble fraction of 80% ethanol or the N of the soluble fraction of 10% trichloroacetic acid was the predominant fraction of N that accumulated in the storage roots. A lack of N supply increased the starch concentration both in the rhizomes and in the storage roots. These results suggested that a high level of N supply to the curcuma plant increased new rhizome formation because of increased flower numbers, but depressed new storage root formation because of reduced starch accumulation., BLACKWELL PUBLISHING
Soil Science and Plant Nutrition, 2006年, ［査読有り］

Long-term effect of nitrate application from lower part of roots on nodulation and N-2 fixation in upper part of roots of soybean (Glycine max (L.) Merr.) in two-layered pot experiment
H Yashima; H Fujikake; A Yamazaki; S Ito; T Sato; K Tewari; N Ohtake; K Sueyoshi; Y Takahashi; T Ohyama, The long-term effect of the concentration and duration of application of nitrate from the lower part of soybean roots on the nodulation and nitrogen fixation in the upper part of roots was investigated using a two-layered pot system separating the upper roots growing in a vermiculite medium and the lower roots growing in a nutrient solution. Continuous absence of nitrate (hereafter referred to as "0-0 treatment"), and continuous 1 mm (1-1 treatment) and 5 mm (5-5 treatment) nitrate treatments were imposed in the lower pot from transplanting to the beginning of the maturity stage. In addition, 5 mm nitrate was supplied partially from the beginning of the pod stage till the beginning of the maturity stage (0-5 treatment) or from transplanting till the beginning of the pod stage (5-0 treatment). The values of the total plant dry weight and seed dry weight were highest in the 5-5 treatment, intermediate in the 1-1, 5-0, 0-5 treatments, and lowest in the 0-0 treatment. The values of the nodule dry weight and nitrogen fixation activity (acetylene reduction activity) were lowest in the 5-5 treatment. The value of the nodule dry weight in the upper roots was highest in the plants subjected to the 1-1 treatment and exceeded that in the 0-0 treatment. Total nitrogen fixation activity of the upper nodules per plant at the beginning of the pod stage was also highest in the 1-1 treatment. These results indicated that long-term supply of a low level of nitrate from the lower roots could promote nodulation and nitrogen fixation in the upper part of roots. Withdrawal of 5 mm nitrate after the beginning of the pod stage (5-0 treatment) markedly enhanced nodule growth and ARA per plant in the upper roots at the beginning of the maturity stage when the values of both parameters decreased in the other treatments. The nitrate concentration in the nodules attached to the upper roots was low, including the 5-5 treatment regardless of the stages of growth. This indicated that the inhibitory effect of 5 mm nitrate or promotive effect of 1 mm nitrate supplied from the lower roots was not directly controlled by nitrate itself, but was mediated by some systemic regulation, possibly by the C or/and N requirement of the whole plant., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
SOIL SCIENCE AND PLANT NUTRITION, 2005年12月, ［査読有り］

¹⁵N analysis of the promotive effect of deep placement of slow-release N fertilizers on growth and seed yield of soybean.
Kaushal T.; Onda M.; Ito S.; Yamazaki A.; Fujikake H.; Ohtake N.; Sueyoshi K.; Takahashi Y. and Ohyama T., It is well known that nodulation and nitrogen fixation of soybean are generally depressed by the application of N fertilizers. We previously reported that a new fertilization method consisting of deep placement of slow-release N fertilizers, such as coated urea and lime nitrogen (calcium cyanamide) at a 20 cm depth enhanced soybean growth and seed yield. In the present study, the effect of deep placement of various N-15-labeled N fertilizers (100 kg N ha(-1)) was compared in converted paddy fields located at Niigata Agricultural Research Institute, in Nagaoka. Fertilizers consisting of N-15-labeled ammonium sulfate (AS), urea (U), coated urea (CU) or lime nitrogen (LN) were applied at a 20 cm depth for nodulated soybean (cv. Enrei) and for a non-nodulated isogenic line (En1282). The plants without deep placement of N fertilizer were used as control (Cont) for both Enrei and En1282. Whole plants were sampled at maturing stage, and N-15 abundance and N concentration in each part were determined. The evaluation of the amounts of Ndfa, Ndfs and Ndff was conducted by the N-15 dilution method using En1282 as a reference plant. Also the amounts of Ndfa and Ndfs+Ndff were estimated by the N balance method for comparing Cont and the other treatments. In all the deep placement treatments, the non-nodulated line exhibited only 36-40% of total dry weight and 16-30% of total N accumulation compared with the nodulated line, due to N deficiency associated with the lack of nitrogen fixation. In both nodulated and non-nodulated soybeans, plant growth and the total amount of accumulated N showed the highest values by the application of LN, followed by CU, U, AS and Cont, respectively: The value of the seed dry weight of Enrei was highest in the LN (73 g plant(-1)), followed by CU (63 g plant(-1)), U (47 g plant(-1)), AS (37 g plant(-1)) and Cont (26 g plant(-1)) treatments. The value of Ndfa estimated by the N-15 dilution method was higher in the LN (3.6 g N plant(-1)) and CU (2.8 g N plant(-1)) treatments than in the U (2.3 g N plant(-1)) and AS (2.2 g N plant(-1)) treatments. In addition, the value of Ndfa estimated by the N-balance method was also higher in the LN (4.1 g N plant(-1)), CU (3.9 g N plant(-1)), U (2.8 g N plant(-1)) and AS (2.6 g N plant(-1)) treatments than in the Cont treatment (1.5 g N plant(-1)). The recovery rate of N fertilizers in Enrei was higher in the LN (43%) and CU (36%) treatments than in the U (21%) treatment and lowest in the AS (8.5%) treatment. These results indicate that deep placement of LN and CU is effective to improve soybean growth and seed yield. The combination of "deep placement" and "slow-release N fertilizers" is important to supply N until the maturation stage, resulting in the increase of seed yield., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
Soil Science and Plant Nutrition, 2005年, ［査読有り］

Quantitative and isotopic analysis of amino acids, allantoin, and allantoic acid in soybeans by LC-MS using the atmospheric pressure chemical ionization method.
Ohtake N.; Takano A.; Ito S.; Yamazaki A.; Fujikake H.; Sueyoshi K. and Ohyama T., In this report, we determined whether liquid chromatography mass spectrometry (LC-MS) using the atmospheric pressure chemical ionization (APCI) method could be applied for isotopic analysis of the high polar compounds, allantoin, and allantoic acid, which are the major transport forms of N from soybean nodules. The peak areas of the ion chromatogram of authentic allantoin and allantoic acid obtained by selected ion monitoring (SIM) were proportional to the contents of the compounds. The data obtained from the ion intensity using the scan mode and the area of the ion chromatogram using the SIM mode were entirely consistent with the isotope ratio of the compounds. The allantoin and allantoic acid concentrations in the xylem sap of soybeans at various growth stages were measured by the following two methods using LC-MS; an absolute calibration method and an isotope-labeled internal standard method. The values estimated by the isotope-labeled internal standard method were more consistent with those obtained by the colorimetric method. Furthermore, amino acids could be detected under the same analytical conditions. The N-15 ratio of asparagine and aspartic acid in the non-nodulating soybean xylem sap which had been supplied with N-15-labeled nitrate could be measured. These results indicate that LC-MS (APCI) is a suitable method for isotopic analysis of high polar compounds, including allantoin and allantoic acid in plant xylem sap and tissues., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
Soil Science and Plant Nutrition, 2004年, ［査読有り］

Effect of placement of urea and coated urea fertilizers on yield and quality of soybean (Glycine max (L.) Merr.) seeds.
Kaushal T.; Onda M.; Ito S.; Yamazaki A.; Fujikake H.; Ohtake N.; Sueyoshi K.; Takahashi Y. and Ohyama T., The objective of the present study was to record the seed yield and to examine visually the quality of soybean seeds cultivated under different types and placements of urea fertilizers. In addition to the conventional fertilizer application (including ammonium sulfate 16 kg N ha(-1)), broadcasting (100 kg N ha(-1)) of urea (UB) and 100-d type coated urea CU-100 (CUB), and deep placement (100 kg N ha(-1)) of urea (UD) and 100-d type coated urea CU-100 (CUD) was conducted in separate plots in a paddy field converted to an upland field located at Shindori Experimental Station of Niigata University. Soybean plant growth was periodically analyzed and the quality of harvested seeds was also visually examined (hereafter referred to as "visual quality"). It was found that the deep placement treatments were more conducive to nitrogen (N,) fixation, based on the relative ureide N concentration in the xylem sap, which is a good indicator of N, fixation by soybean. Also the total seed yield was the highest in CUD (82 g plant(-1)) and UD (81 g plant(-1)), compared to the control (62 g plant(-1)), UB (68 g plant(-1)), and CUB (68 g plant(-1)). The visual quality of harvested seeds showed that CUD enhanced the quality of seeds compared to the other treatments, in which the percentage of good quality seeds, hereafter referred to as "good seeds," based on the dry weight was 51 (control), 65 (UB), 61 (CUB), 61 (UD), and 66% (CUD). In terms of diseased seeds, the percentage of turtle wrinkle and broken seed coats was found to decrease by N application compared to the control. Thus, it is suggested that N fertilization management is important for maximum yield of soybean as well as for the enhancement of seed quality., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
Soil Science and Plant Nutrition, 2004年, ［査読有り］

水熱分解技術の有害鳥獣処理への適用
七夕 小百合、杤本 信彦、藤原 俊六郎
JATAFFジャーナル, 2019年11月01日, ［招待有り］
筆頭著者

Recycling of nitrogen from shoots to underground parts in hypernodulation mutant lines of soybean by sprit-root experiment.
Ohyama T; Ueno M; Ono Y; Ohtake N; Sueyoshi K; Sato T; Tanabata S
Bull. Facul. Agric. Niigata Univ., 2018年

根圏イメージングによるカドミウム動態のイネ品種間差の解析
鈴井伸郎; 石川覚; 七夕小百合; 石井里美; 井倉将人; 安部匡; 河地有木; 藤巻秀
日本土壌肥料学会講演要旨集, 2013年

Leaf growth of Lotus japonicus hypernodulation mutant har1-4.
Tanabata S; Ohtake N; Sueyoshi K; Kawaguchi M; Ohyama T
Bull. Facul. Agric. Niigata Univ., 2013年

飼料用米生産における豚尿由来液状コンポストの窒素肥効特性．
須藤立,七夕小百合,大林康信,眞部幸子,井上雅美,矢萩久嗣
茨城県畜産センター研究報告, 2012年

Molecular imaging for plant physiology: Imaging of carbon translocation to sink organs
N. Kawachi; N. Suzui; S. Ishii; S. Ito; N. S. Ishioka; K. Kikuchi; T. Tsukamoto; T. Kusakawa; F. Fujimaki
IEEE Nuclear Science Symposium Conference Record, 2009年, ［査読有り］

Allocation of photosynthetic products in hypernodulation mutant of soybean NOD1-3 in the early stage of nodule formation.
Ito S; Ohtake N; Sueyoshi K; Ohyama T
Bull. Facul. Agric. Niigata Univ., 2006年

Comparison of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, and their parent cv. Williams.
Ito S; Ohtake N; Sueyoshi K; Ohyama T
Bull. Facul. Agric. Niigata Univ., 2006年

Nitrogen Fertilization Using Drip Fertigation for Cucumber Cultivation Based on Leaf-Count Technique　　　　　　　　　　　　　　　
Tatsuo Sato; Yuki Tashiro; Sayuri Tanabata, 共著
IntechOpen, 2023年10月

Amino Acid Metabolism and Transport in Soybean Plants,Chapter 8 "Amino Acid - New Insights and Roles in Plant and Animal"
Takuji Ohyama; Norikuni Ohtake; Kuni Sueyoshi; Yuki Ono; Kotaro Tsutsumi; Manabu Ueno; Sayuri Tanabata; Takashi Sato and Yoshihiko Takahashi, 共著
Intech Open, 2017年06月

Role of Nitrogen on Growth and Seed Yield of Soybean and a New Fertilization Technique to Promote Nitrogen Fixation and Seed Yield,Chapter 9 "Amino Acid - New Insights and Roles in Plant and Animal"
Takuji Ohyama; Kaushal Tewari; Shinji Ishikawa; Kazuya Tanaka; Satoshi Kamiyama; Yuki Ono; Soshi Hatano; Norikuni Ohtake; Kuni Sueyoshi; Hideo Hasegawa; Takashi Sato; Sayuri Tanabata; Yoshifumi Nagumo; Yoichi Fujita and Yoshihiko Takahashi, 共著
Intech Open, 2017年06月

Advances in Biology and Ecology of Nitrogen Fixation.,Chapter 4 "Autoregulation of nodulation in soybean plants."　　　　　　　　　　　　　　　
Tanabata S. and Ohyama T., 共著
InTech, 2014年

Nitrogen Assimilation in Plants,"Regulation of root nodule formation in leguminous plants."　　　　　　　　　　　　　　　
Ito S.; Fujimaki S.; Kato T.; Ohtake N.; Sueyoshi K. and Ohyama T., 共著
Research Signpost, 2010年

Case study on cucumber transportation using modified atmosphere packaging to prevent postharvest deformation, blossom-end enlargement　　　　　　　　　　　　　　　
Yuki Tashiro; Kohei Mochizuki; Erika Uji; Rina Ito; Tran Mi Quyen; Nur Akbar Arofatullah; Agung Dian Kharisma; Sayuri Tanabata; Tatsuo Sato
Postharvest 2024, VII International Symposium
20241111, 20241115

Underlying mechanism of a postharvest deformation, blossom end-enlargement in cucumber fruits　　　　　　　　　　　　　　　
Li Rui; Runewa Atarashi; Agung Dian Kharisma; Nur Akbar Arofatullah; Yuki Tashiro; Junjira Satitmunnaithum; Sayuri Tanabata; Kenji Yamane; Tatsuo Sato
Postharvest 2024, VII International Symposium
20241111, 20241115

サツマイモ育苗における種いもの働きの解明　　　　　　　　　　　　　　　
吉田 穣; 高松 好; サティッマンナイタム ジャンジラ; 浅木直美; 七夕小百合; 佐藤達雄
園芸学会令和6年度秋季大会, 2024年11月04日, 園芸学会
20241103, 20241105

キュウリ先端肥大症の発生に対する植物ホルモンの影響　　　　　　　　　　　　　　　
小松澤一茶; 黄 洁榕; 李 蕊; 田代勇樹; 七夕小百合; サティッマンナイタム ジャンジラ; 佐藤達雄
園芸学会令和6年秋季大会, 2024年11月04日, 園芸学会
20241103, 20241105

Analysis of the Role of GmNARK in Soybean using Hypernodulation NOD Mutant Lines　　　　　　　　　　　　　　　
Haruka Yoshiizumi; Ayako Furutani; Tatsuo Sato; Gary Stacey; Sayuri Tanabata
2024 IPG Symposium, 2024年05月21日
20240521, 20240524

切創が甘藷種いもの萌芽に及ぼす影響　　　　　　　　　　　　　　　
畠山いづみ; 吉田 穣; 高松 好; 棚谷智寿; 岩崎 明; 七夕小百合; 浅木直美; 佐藤達雄
日本作物学会, 2023年09月
20230914, 20230915

甘藷の空中採苗法の検討　　　　　　　　　　　　　　　
高松 好; 名雪藍那; 畠山いずみ; 吉田 穣; 棚谷智寿; 岩崎 明; 七夕小百合; 浅木直美; 佐藤達雄
日本作物学会, 2023年09月
20230914, 20230915

Causes of Postharvest Deformation “Blossom-End Enlargement” in Cucumber　　　　　　　　　　　　　　　
Rui Li; Runewa Atarashi; Agung Dian Kharisma; Junjira Satitmunnaithum; Sayuri Tanabata; Tatsuo Sato
American Society for Horticultural Science 2023 Annual Conference, 2023年08月01日
20230731, 20230804

ダイズ根粒超着生変異株NOD系統の原因遺伝子と表現型の解析　　　　　　　　　　　　　　　
吉泉春華・古谷綾子・佐藤達雄・七夕小百合
日本土壌肥料学会関東支部大会（茨城大会）, 2022年11月20日

ダイズにおける根粒着生形態の違いが生育に及ぼす影響　　　　　　　　　　　　　　　
李 建霖; 佐藤達雄; 七夕小百合
日本土壌肥料学会, 2018年08月

水熱分解液肥がイネの生育および収量に及ぼす影響　　　　　　　　　　　　　　　
土田直希; 蜷木朋子; 小沢聖; 藤原俊六郎; 七夕小百合
日本土壌肥料学会 関東支部講演会, 2017年11月

水熱分解液肥がコマツナの生育に及ぼす影響　　　　　　　　　　　　　　　
七夕小百合; 岡部 勝美; 蜷木 朋子; 小沢 聖; 藤原 俊六郎
日本土壌肥料学会, 2017年09月

キュウリにおける栽培法の違いが窒素・炭素の移行に及ぼす影響　　　　　　　　　　　　　　　
七夕小百合; 浅木 直美; 山口 貴之; 田代 勇樹; 下村 晃一郎; 佐藤 達雄
日本土壌肥料学会, 2016年09月

野菜残渣水熱分解液肥が作物生育に及ぼす影響（第２報）　　　　　　　　　　　　　　　
七夕小百合、小沢聖、藤原俊六郎
日本土壌肥料学会, 2015年09月

野菜残渣水熱分解液肥が作物生育に及ぼす影響　　　　　　　　　　　　　　　
七夕小百合、小沢聖、藤原俊六郎
日本土壌肥料学会, 2014年09月

ダイズ子実のカドミウム汚染のメカニズム〜ポジトロンイメージング技術×植物栄養学〜　　　　　　　　　　　　　　　
伊藤小百合、鈴井伸郎、河地有木、石岡典子、藤巻秀
第13回放射線プロセスシンポジウム, 2009年11月

Characterization of cadmium absorption and transport in soybean plant using radioisotopes ¹⁰⁷Cd and ¹⁰⁹Cd.　　　　　　　　　　　　　　　
Ito S; Suzui N; Kawachi N; Ishioka N.S; Fujimaki S
MARCO (Monsoon Asia Agro-Environmental Research Consortium) Symposium, 2009年10月

ダイズ植物におけるカドミウム吸収および輸送の特性　　　　　　　　　　　　　　　
伊藤小百合、鈴井伸郎、河地有木、石岡典子、藤巻秀
第4回高崎量子応用研究シンポジウム, 2009年10月

¹⁰⁷Cdと¹⁰⁹Cdを用いたダイズ子実へのカドミウム移行の生育ステージ毎の解析　　　　　　　　　　　　　　　
伊藤小百合、鈴井伸郎、河地有木、石岡典子、藤巻秀
日本土壌肥料学会, 2009年09月

Characterization of cadmium transport in soybean plant using radioisotopes ¹⁰⁷Cd and ¹⁰⁹Cd.　　　　　　　　　　　　　　　
Ito S; Suzui N; Kawachi N; Ishioka N.S; Fujimaki S
16th International Plant Nutrition Colloquium, 2009年08月

Physiological analysis of the autoregulation of nodulation in soybean.　　　　　　　　　　　　　　　
Ito S; Kato T; Ohtake N; Sueyoshi K; Ohyama T
The 2nd International symposium on Food Security, Agricultural Development & Environmental Conservation in southeast and east Asia, 2007年09月

Comparison of leaf growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7 with their parent cv. Williams.　　　　　　　　　　　　　　　
Ito S; Ohtake N; Sueyoshi K; Ohyama T
15th international congress on nitrogen fixation & 12th international conference of the African association for biological nitrogen fixation, 2007年01月

ダイズ品種Williamsと根粒超着生変異株（NOD系統）の地上部初期生育の比較　　　　　　　　　　　　　　　
伊藤小百合、大竹憲邦、末吉邦、大山卓爾
植物微生物研究会, 2006年09月

ダイズ根粒超着生変異株NOD系統の表現型の比較　　　　　　　　　　　　　　　
伊藤小百合、大竹憲邦、末吉邦、大山卓爾
日本土壌肥料学会, 2006年09月

Allocation of photosynthetic products in soybean during the early stage of nodule formation.　　　　　　　　　　　　　　　
Ito S; Kato T; Ohtake N; Sueyoshi K; Ohyama T
JSPS/NSF Joint seminar: Genetic approach to elucidate molecular mechanism of symbiotic nitrogen fixation, 2006年08月

ダイズ根粒形成過程における光合成産物の分配　　　　　　　　　　　　　　　
伊藤小百合、大竹憲邦、末吉邦、大山卓爾
植物微生物研究会, 2005年09月

¹³C標識光合成産物のイネの穂への移動に対する高温ストレスの影響　　　　　　　　　　　　　　　
伊藤小百合、原武、木村拓也、大竹憲邦、高橋能彦、佐藤孝、佐藤敦、福山利範、渡邉敏行、三ツ井敏明、大山卓爾
日本土壌肥料学会, 2005年09月

ダイズ根粒形成初期過程における光合成産物の分配　　　　　　　　　　　　　　　
伊藤小百合、大竹憲邦、山﨑明彦、末吉邦、大山卓爾
日本土壌肥料学会, 2004年09月

ダイズ根粒形成初期過程における光合成産物の分配　　　　　　　　　　　　　　　
伊藤小百合、大竹憲邦、山﨑明彦、大山卓爾、末吉邦、久米民和、藤巻秀、石岡典子、渡辺智、松橋信平
理工学における同位元素・放射線研究発表会, 2004年07月

2004年, 日本土壌肥料学会