オバタ ユイ
小畑 結衣教育研究振興教員(助教)
Yui Obata

■研究者基本情報

組織

  • 理学部 理学科 化学コース
  • 基礎自然科学野 化学領域

学位

  • 2023年03月 博士(工学)(茨城大学)

経歴

  • 2024年04月 - 現在, 茨城大学, 基礎自然科学野, 教育研究振興教員
  • 2023年04月 - 2024年03月, 東京大学, 工学系研究科原子力専攻, 特任研究員

委員歴

  • 2024年04月 - 現在, 若手部会 役員, 日本放射線影響学会

■研究活動情報

受賞

  • 2021年09月, 優秀演題発表賞, Transfection efficiency depending on distance between a double-strand break site and EGFP-gene in plasmid DNA, 日本放射線影響学会第64回大会
    小畑結衣;木名瀨暁理;秋光信佳;横谷明徳
  • 2020年10月, 優秀演題発表賞, Development of a vector DNA system for visualization of intracellular repair dynamics of DNA damage by ionizing radiation, 日本放射線影響学会第63回大会
    小畑結衣;平嵜敬志朗;秋光信佳;鈴木雅雄;横谷明徳

論文

  • Hydroxyl radical scavenging and chemical repair capabilities of positively charged peptides (PCPs): a pulse radiolysis study
    Chaozhong Tian; Shinichi Yamashita; Atsushi Kimura; Yui Obata; Hao Yu; Mitsumasa Taguchi, Informa UK Limited
    Free Radical Research, 2024年07月02日
  • Expression of an X-Ray Irradiated EGFP-Expressing Plasmid Transfected into Nonirradiated Human Cells.
    Yui Obata; Hiroki Nakaue; Keishiro Hirasaki; Nobuyoshi Akimitsu; Akinari Yokoya, To investigate the repairability of X-ray induced DNA damage, particularly non-double-strand breaks in living cells, enhanced green fluorescent protein (EGFP)-expressing plasmids X-ray irradiated and then transfected into nonirradiated human cells, MCF7 and MCF10A. Live-cell imaging of EGFP fluorescence was performed to measure the efficiency of plasmid repair in cells. The number of EGFP-expressing cells significantly decreased with increasing X-ray dose for both cell lines. The obtained kinetic curves of EGFP expression indicating plasmid repair were quantitatively compared against algebraically calculated ones based on the values of the transfected plasmids that had been treated with nicking or restriction enzymes. Then, assuming a Poisson distribution of single-strand breaks (SSBs), the number of cells carrying these nicked plasmids that could express EGFP were estimated. Our experimental results revealed considerably fewer cells expressing EGFP compared to the expected values we had calculated. These results suggest that the lower proportion of cells expressing EGFP as a measure of plasmid repair was due not only to the complex chemical structures of termini created by SSBs compared to those created by enzyme treatments, but also that base lesions or AP sites proximately arising at the strand-break termini might compromise EGFP expression. These results emphasize that radiation-induced DNA breaks are less repairable than enzymatically induced DNA breaks, which is not apparent when using conventional gel electrophoresis assays of plasmid DNA.
    Radiation research, 2021年09月01日
  • VISUALIZATION OF THE DNA REPAIR PROCESS IN MAMMALIAN CELLS TRANSFECTED WITH EGFP-EXPRESSING PLASMID DNA AFTER EXPOSURE TO X-RAYS IN VITRO.
    Hiroki Nakaue; Yui Obata; Kiichi Kaminaga; Nobuyoshi Akimitsu; Akinari Yokoya, To investigate the repair process of DNA damage induced by ionizing radiation in isolation from various types of cytoplasmic damage, we transfected X-irradiated enhanced green fluorescent protein (EGFP)-expressing plasmid DNA into non-irradiated mammalian cells using lipofectamine. The repair kinetics of the irradiated plasmids in the cells were visualized under microscopy as the EGFP fluorescence emitted by transfected cells. Using an agarose gel electrophoresis method, the yields of single- and double-strand breaks of the plasmids were also quantified. As positive control experiments, plasmid DNA with single- or double-strand breaks induced by a nicking or restriction enzyme were also transfected into the cells. The DNA repair rates for X-ray-irradiated plasmids were significantly lower than those of the enzymatically digested positive control samples. These results indicate that X-rays could induce less repairable damage than that induced by enzymes.
    Radiation protection dosimetry, 2019年05月01日

MISC

  • 酵素的に二本鎖切断を誘発したEGFPプラスミドDNAのin vivoおよびin vitro再結合の可能性の検討
    江田脩真; 伊東裕真; 小畑結衣; 廣瀬エリ; 横谷明徳; 横谷明徳
    アイソトープ・放射線研究発表会(Web), 2024年
  • ハロゲン化ヌクレオチド(Br-dUMP)と放射線誘発水分解生成物との反応のパルスラジオリシス研究
    小畑 結衣; 伊東 祐真; 横谷 明徳; 山下 真一
    日本放射線影響学会大会講演要旨集, 2023年11月
  • 捕捉剤フリーなプラスミド DNA 希薄水溶液への X 線照射で誘発される鎖切断と塩基損傷の収率
    小畑 結衣; 篠原 俊輔; 田 超中; 山下 真一
    第66回放射線化学討論会, 2023年09月
  • リンK殻共鳴励起によるEGFP発現プラスミドDNAの損傷と細胞内における修復
    小畑結衣; 木名瀬暁理; 横谷明徳; 横谷明徳
    量子ビームサイエンスフェスタ(Web), 2023年
  • ヒト細胞中に導入された線形プラスミドDNAは遺伝子発現をするのか?(Does linear plasmid DNA express genes in it after transfected into human cells)
    小畑 結衣; 木名瀬 暁理; 伊東 祐真; 秋光 信佳; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2022年09月
  • 軟X線を照射したEGFP発現プラスミドDNAの非照射細胞内におけるDNA修復の難易性(DNA repair susceptibility in non-irradiated cells transfected with EGFP expressing plasmid DNA irradiated with various soft X-rays)
    木名瀬 暁理; 小畑 結衣; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2022年09月
  • Brから放出される低エネルギー二次電子がプラスミドDNAの鎖切断に与える効果(Effect of low-energy secondary electrons emitted from bromine atoms on the strand breaks of plasmid DNA)
    伊東 祐真; 小畑 結衣; 木名瀬 暁理; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2022年09月
  • 放射線損傷DNAの細胞内修復ダイナミクス可視化のための新たなベクターDNA系の開発(Development of a vector DNA system for visualization of intracellular repair dynamics of DNA damage by ionizing radiation)
    小畑 結衣; 平嵜 敬志朗; 秋光 信佳; 鈴木 雅雄; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2020年10月
  • 放射線損傷DNAの細胞内修復ダイナミクス可視化のための新たなベクターDNA系の開発(Development of a vector DNA system for visualization of intracellular repair dynamics of DNA damage by ionizing radiation)
    小畑 結衣; 平嵜 敬志朗; 秋光 信佳; 鈴木 雅雄; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2020年10月
  • 水和水に対するX線エネルギー付与により誘発されたEGFPプラスミドDNA損傷の修復のライブセルイメージング(Live cell imaging of repair of EGFP plasmid DNA damage induced by X-ray energy deposition on hydrated waters)
    平嵜 敬志朗; 小畑 結衣; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2020年10月
  • 非照射ヒト細胞におけるin vitro X線照射プラスミドDNAのEGFP発現のライブセル観察(Live cell observation of EGFP expression of in vitro X-irradiated plasmid DNA in non-irradiated human cells)
    小畑 結衣; 平嵜 敬志朗; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2019年11月
  • ヒト細胞中に移入されたX線照射水和EGFP発現プラスミドDNAの修復感受性(Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays)
    平嵜 敬志朗; 小畑 結衣; 横谷 明徳
    日本放射線影響学会大会講演要旨集, 2019年11月

講演・口頭発表等

  • DNA repair response of mammary gland cells to EGFP plasmid DNA exposed to various ionizing radiations or treated with restriction enzymes               
    Obata Y; Kinase A; Hirasaki K; Akimitsu N; Yokoya A
    The Radiation Research Society's 68th Annual Meeting, 2022年10月17日
    20221016, 20221019
  • Measurement of irradiation plasmid DNA repair rate in non-irradiated human cells.               
    Obata Y; Hirasaki K; Akimitsu N; Yokoya A
    University Training program to Australian Nuclear Science and Technology Organisation,, 2020年03月12日
  • Live cell observation of DNA repair using EGFP plasmid exposed to X-rays in various conditions.               
    Hirasaki K; Obata Y; Yokoya A
    3th QST International Symposium Quantum Life Science, 2019年12月04日
    20191204, 20191205
  • Repair susceptibility of X-irradiated EGFP plasmid DNA transfected into non-irradiated cells.               
    Obata Y; Hirasaki K; Akimitsu N; Suzuki M; Yokoya A
    3th QST International Symposium Quantum Life Science, 2019年12月04日
    20191204, 20191205
  • Live cell observation of expression of X-irradiated EGFP-plasmid DNA in non-irradiated human cells.               
    Obata Y; Hirasaki K; Akimitsu N; Yokoya A
    The 16th International Congress of Radiation Research (ICRR 2019), 2019年08月27日
    20190825, 20190829
  • A. Live cell observation of repair of EGFP-plasmid DNA damage induced by X-irradiation               
    Obata Y; Nakaue H; Hirasaki K; Akimitsu N; Suzuki M; Yokoya, A
    Ibaraki University Training program to Australian Nuclear Science and Technology Organisation, 2019年01月30日
    20190129, 20190131
  • A novel method to visualize DNA repair process in mammalian cells transformed by plasmid DNA exposed to various quantum beams.               
    Nakaue H; Obata Y; Kaminaga K; Yokoya A
    1st QST International Symposium Quantum Life Science
    20170725, 20170726
  • Visualization of DNA repair process in mammalian cells transformed by plasmid DNA exposed to X-ray in vitro               
    Nakaue H; Obata Y; Kaminaga K; Yokoya A
    17th International Symposium on Microdosimetry on MICROS 2017
    20170705, 20170710

所属学協会

  • 2024年10月 - 現在, プラズマ・核融合学会
  • 2023年09月 - 現在, 日本放射線化学会
  • 2018年09月 - 現在, 日本放射線影響学会

共同研究・競争的資金等の研究課題

  • トリチウム誘発DNA損傷と細胞内修復応答の研究
    若手研究
    茨城大学
    2025年04月01日 - 2028年03月31日
  • OBT生成反応における同位体効果の検証               
    茨城大学
    2025年04月 - 2026年03月
  • 生体への水素同位体取込みの同位体効果の検証               
    茨城大学
    2024年04月 - 2025年03月