Mar. 2014, ベストポスター賞, Formation of neuronal circuits by interactions between neuronal populations derived from different origins in the Drosophila visual center, 動く細胞と場のクロストーク 若手の会
Japan society

Systemic positive feedback regulation of ecdysone biosynthesis via sugar metabolism in insects
Takumi Suzuki; Shi-Hong Gu, Lead
Developmental Biology, Sep. 2025, [Reviewed]

bHLH family proteins control the timing and completion of transition from neuroepithelial cells into neural stem cells
Chika Akiba; Aya Takezawa; Yuanchang Tsai; Mire Hirose; Takumi Suzuki, Last, ABSTRACT

The number of neural stem cells reflects the total number of neurons in the mature brain. As neural stem cells arise from neuroepithelial cells, the neuroepithelial cell population must be expanded to secure a sufficient number of neural stem cells. However, molecular mechanisms that regulate timely differentiation from neuroepithelial to neural stem cells are largely unclear. Here, we show that TCF4/Daughterless is a key factor that determines the timing of the differentiation in Drosophila. The neuroepithelial cells initiated but never completed the differentiation in the absence of TCF4/Daughterless. We also found that TCF4/Daughterless binds to the Notch locus, suggesting that Notch is one of its downstream candidate genes. Consistently, Notch expression was ectopically induced in the absence of TCF4/Daughterless. Furthermore, ectopic activation of Notch signaling phenocopied loss of TCF4/Daughterless. Our findings demonstrate that TCF4/Daughterless directly inactivates Notch signaling pathway, resulting in completion of the differentiation from neuroepithelial cells into neural stem cells with optimal timing. Thus, the present results suggest that TCF4/Daughterless is essential for determining whether to move to the next state or stay in the current state in differentiating neuroepithelial cells., The Company of Biologists
Development, 15 Sep. 2024, [Reviewed]

Steroid hormone-dependent changes in trehalose physiology in the silkworm, Bombyx mori
Suzuki; T.; Akiba; C.; Izawa; M. and Iwami; M., Lead, Holometabolous insects undergo metamorphosis to reconstruct their body to the adult form during pupal period. Since pupae cannot take any diets from the outside because of a hard pupal cuticle, those insects stock up on nutrients sufficient for successful metamorphosis during larval feeding period. Among those nutrients, carbohydrates are stored as glycogen or trehalose, which is the major blood sugar in insects. The hemolymph trehalose is constantly high during the feeding period but suddenly decreases at the beginning of the prepupal period. It is believed that trehalase, which is a trehalose-hydrolyzing enzyme, becomes highly active to reduce hemolymph trehalose level during prepupal period. This change in the hemolymph trehalose level has been interpreted as the physiological shift from storage to utilization of trehalose at that stage. Although this shift in trehalose physiology is indispensable for energy production required for successful metamorphosis, little is known on the regulatory mechanisms of trehalose metabolism in accordance with developmental progress. Here, we show that ecdysone, an insect steroid hormone, plays essential roles in the regulation of soluble trehalase activity and its distribution in the midgut of silkworm, Bombyx mori. In the end of larval period, soluble trehalase was highly activated in the midgut lumen. This activation was disappeared in the absence of ecdysone and also restored by ecdysone administration. Our present results suggest that ecdysone is essentially required for the changes in the function of the midgut on trehalose physiology as development progresses., Springer Nature
Journal of Comparative Physiology B, 23 May 2023, [Reviewed]

Cutting edge technologies expose the temporal regulation of neurogenesis in the Drosophila nervous system
M. Sato and T. Suzuki, Informa UK Limited
Fly, 13 May 2022, [Reviewed]

NanoDam identifies Homeobrain and Scarecrow as conserved temporal factors in the Drosophila central brain and visual system　　　　　　　　　　　　　　　
Tang; J.; Hakes; A.; Krautz; R.; Suzuki; T.; Contreras; E.G.; Fox; P.; and Brand; A.H.
Developmental Cell, 09 May 2022, [Reviewed]

Sequential changes in the regulatory mechanism of carbohydrate digestion in larvae of the silkworm, Bombyx mori
Takumi Suzuki; Masafumi Iwami, Lead
Journal of Comparative Physiology B, May 2021, [Reviewed]

Ecdysteroid ingestion suppresses carbohydrate hydrolysis in larvae of the silkworm Bombyx mori
Takumi Suzuki; Masafumi Iwami, Lead, Springer Science and Business Media LLC
Naturwissenschaften, Aug. 2020, [Reviewed]

Netrin Signaling Defines the Regional Border in the Drosophila Visual Center.
Suzuki T; Liu C; Kato S; Nishimura K; Takechi H; Yasugi T; Takayama R; Hakeda-Suzuki S; Suzuki T; Sato M, The brain consists of distinct domains defined by sharp borders. So far, the mechanisms of compartmentalization of developing tissues include cell adhesion, cell repulsion, and cortical tension. These mechanisms are tightly related to molecular machineries at the cell membrane. However, we and others demonstrated that Slit, a chemorepellent, is required to establish the borders in the fly brain. Here, we demonstrate that Netrin, a classic guidance molecule, is also involved in the compartmental subdivision in the fly brain. In Netrin mutants, many cells are intermingled with cells from the adjacent ganglia penetrating the ganglion borders, resulting in disorganized compartmental subdivisions. How do these guidance molecules regulate the compartmentalization? Our mathematical model demonstrates that a simple combination of known guidance properties of Slit and Netrin is sufficient to explain their roles in boundary formation. Our results suggest that Netrin indeed regulates boundary formation in combination with Slit in vivo.
iScience, Oct. 2018, [Reviewed]

Inter-progenitor pool wiring: An evolutionarily conserved strategy that expands neural circuit diversity
Takumi Suzuki; Makoto Sato
DEVELOPMENTAL BIOLOGY, Nov. 2017, [Reviewed]

Wnt Signaling Specifies Anteroposterior Progenitor Zone Identity in the Drosophila Visual Center
Takumi Suzuki; Olena Trush; Tetsuo Yasugi; Rie Takayama; Makoto Sato
JOURNAL OF NEUROSCIENCE, Jun. 2016, [Reviewed]

Formation of Neuronal Circuits by Interactions between Neuronal Populations Derived from Different Origins in the Drosophila Visual Center
Takumi Suzuki; Eri Hasegawa; Yasuhiro Nakai; Masako Kaido; Rie Takayama; Makoto Sato
CELL REPORTS, Apr. 2016, [Reviewed]

eyeless/Pax6 controls the production of glial cells in the visual center of Drosophila melanogaster
Takumi Suzuki; Rie Takayama; Makoto Sato
DEVELOPMENTAL BIOLOGY, Jan. 2016, [Reviewed]

Neurogenesis and neuronal circuit formation in the Drosophila visual center
Takumi Suzuki; Makoto Sato
DEVELOPMENT GROWTH & DIFFERENTIATION, Sep. 2014, [Reviewed]

Waves of differentiation in the fly visual system
Makoto Sato; Takumi Suzuki; Yasuhiro Nakai
DEVELOPMENTAL BIOLOGY, Aug. 2013, [Reviewed]

A temporal mechanism that produces neuronal diversity in the Drosophila visual center
Takumi Suzuki; Masako Kaido; Rie Takayama; Makoto Sato
DEVELOPMENTAL BIOLOGY, Aug. 2013, [Reviewed]

Steroidal regulation of hydrolyzing activity of the dietary carbohydrates in the silkworm, Bombyx mori
Takumi Suzuki; Sho Sakurai; Masafumi Iwami
JOURNAL OF INSECT PHYSIOLOGY, Sep. 2011, [Reviewed]

Juvenile hormone delays the initiation of rectal sac distention by disrupting ecdysteroid action in the silkworm, Bombyx mori
Takumi Suzuki; Sho Sakurai; Masafumi Iwami
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, Jul. 2010, [Reviewed]

Physiological requirements for 20-hydroxyecdysone-induced rectal sac distention in the pupa of the silkworm, Bombyx mori
Takumi Suzuki; Sho Sakurai; Masafumi Iwami
JOURNAL OF INSECT PHYSIOLOGY, Jun. 2010, [Reviewed]

Rectal sac distention is induced by 20-hydroxyecdysone in the pupa of Bombyx mori
Takumi Suzuki; Sho Sakurai; Masafumi Iwami
JOURNAL OF INSECT PHYSIOLOGY, Mar. 2009, [Reviewed]

神経上皮細胞から神経幹細胞への分化 タイミングを決定する分子機構の理解　　　　　　　　　　　　　　　
鈴木 匠
Brain and Spinal cord, Feb. 2025, [Invited]
Lead

Inter-progenitor pool wiring : an evolutionarily conserved strategy that expands neural circuit diversity
Sato, M.; Suzuki, T.
生化学, Dec. 2017, [Reviewed]

Hemolymph Proteins and Functional Peptides: Recent Advances in Insects and Other Arthropods　　　　　　　　　　　　　　　
Takumi Suzuki; Masafumi Iwami, Contributor
Bentham Science Publishers, 2012

Studeies on the regulation mechanism of sugar metabolism during the larval-pupal transition of the silkworm, Bombyx mori.
鈴木 匠
[Takumi Suzuki], 2011

ハエ視覚中枢のフィードバックニューロンの形態形成機構の解明　　　　　　　　　　　　　　　
伊澤 実咲; 鈴木 匠
第48回日本分子生物学会, 28 Nov. 2024
20241127, 20241129

Daughterlessによる神経上皮細胞から神経幹細胞への分化制御　　　　　　　　　　　　　　　
秋庭 知佳、武澤 彩、鈴木 匠
第48回日本分子生物学会, 27 Nov. 2024
20241127, 20241129

Daughterless is essential to complete differentiation from neuroepithelial cells into neural stem cells in the fruit fly, Drosophila melanogaster　　　　　　　　　　　　　　　
Chika Akiba, Aya Takezawa, Yuanchang Tsai, Takumi Suzuki
27th International Congress of Entomology (ICE2024 Kyoto), 26 Aug. 2024
20240825, 20240830

Daughterless is essential to complete differentiation from neuroepithelial cells into neural stem cells　　　　　　　　　　　　　　　
Chika Akiba; Aya Takezawa; Yuanchang Tsai; Mire Hirose; Takumi Suzuki
第57回 日本発生生物学会, 19 Jun. 2024
20240620, 20240622

Daughterless is essential to complete differentiation from neuroepithelial cells into neural stem cells　　　　　　　　　　　　　　　
Chika Akiba; Yuanchang Tsai; Aya Takezawa; Mire Hirose; Takumi Suzuki
APDNC3, 28 Feb. 2024
20240227, 20240301

Molecular mechanisms that regulate neuronal differentiation by Extramacrochaete　　　　　　　　　　　　　　　
Gensyo Sai; Aya Takezawa; and Takumi Suzuki
第45回 日本分子生物学会, 02 Dec. 2022

Identifying genes that regulate the production of neurogenesis diversity in fly visual center　　　　　　　　　　　　　　　
Akiba Chika; Izawa Misaki; Saito Anna; Aya Takezawa and Takumi Suzuki
第45回 日本分子生物学会, 01 Dec. 2022

Klumpfuss guarantees neuronal differentiation in two different stem cell pools in Drosophila visual center　　　　　　　　　　　　　　　
Takumi Suzuki
The 7th Visual System Neuron Meeting, 22 Nov. 2022

Snail family transcription factors are involved in the transition from neuroepithelial to neural stem cells　　　　　　　　　　　　　　　
Anna Saito and Takumi Suzuki
The 7th Visual System Neuron Meeting, 22 Nov. 2022

Identifying genes that regulate the production of neuronal diversity in Drosophila visual center　　　　　　　　　　　　　　　
Yuanchang Tsai; Akiba Chika; Izawa Misaki; Saito Anna; *Takumi Suzuki
第55回 日本発生生物学会, Jun. 2022

Identifying genes that regulate neuronal diversity in Drosophila visual system　　　　　　　　　　　　　　　
Akari Tanaka; Yuanchang Tsai; Naho Tsubota; Takumi Suzuki
第44回 日本分子生物学会年会, Dec. 2021

Identifying genes that regulate neural stem cell quiescence　　　　　　　　　　　　　　　
Takumi Suzuki and Andrea H. Brand
第43回 日本分子生物学会年会, 02 Dec. 2020

Reactivation of quiescent neural stem cell via cholinergic signaling　　　　　　　　　　　　　　　
Takumi Suzuki and Andrea H. Brand
5th Visual System Neuron Meeting, 11 Nov. 2020, Makoto Sato
20201111, 20201112

Identifying genes that regulate neural stem cell quiescence　　　　　　　　　　　　　　　
第42回 日本分子生物学会年会, Dec. 2019

Roles of unfolded protein response signaling in the regulation of neural stem cell reactivation　　　　　　　　　　　　　　　
Takumi Suzuki; Andrea Brand
25th European Drosophila Research Conference, Sep. 2017

Formation of neuronal circuits by interactions between neuronal populations derived from different origins in the Drosophila visual center　　　　　　　　　　　　　　　
Takumi Suzuki; Eri Hasegawa; Yasuhiro Nakai; Masako Kaido; Rie Takayama; Makoto Sato
24th European Drosophila Research Conference, Sep. 2015

Formation of neuronal circuits by interactions between neuronal populations derived from different origins in the Drosophila visual center.　　　　　　　　　　　　　　　
鈴木匠; 海道雅子; 高山理恵; 佐藤純
発生生物学会第48回大会, Jun. 2015

Neuronal expression of eyeless/Pax6 controls the production of glial cells　　　　　　　　　　　　　　　
鈴木匠; 高山理恵; 佐藤純
日本分子生物学会第37回大会, Nov. 2014

Establishment of neural circuit by interaction between cells of different origins　　　　　　　　　　　　　　　
鈴木匠; 海道雅子; 高山理恵; 佐藤純
Japanese Drosophila Research Conference 11, Jun. 2014

Molecular basis of the production of neuronal diversity in the Drosophila visual center　　　　　　　　　　　　　　　
Takumi Suzuki; Masako Kaido; Rie Takayama; Makoto Sato
53rd Annual Drosophila Research Conference, Mar. 2012

Aug. 2011, 日本分子生物学会

Apr. 2011, Japanese Society for Developmental Biology

Apr. 2011, 国際発生生物学会

神経幹細胞が多種多様な神経を作り分ける分子機構の解明　　　　　　　　　　　　　　　
Aug. 2022 - Jul. 2027

神経上皮細胞から神経幹細胞への分化時期を決定する分子基盤の理解　　　　　　　　　　　　　　　
基盤研究（C）
Apr. 2024 - Mar. 2027

ゲノム上の疾病原因箇所を迅速に特定する技術の開発　　　　　　　　　　　　　　　
Jan. 2024 - Mar. 2026

グリア細胞由来の栄養シグナルによる神経の細胞死抑制機構を理解する　　　　　　　　　　　　　　　
Dec. 2023 - Sep. 2025

神経上皮細胞の増殖期を終結させる分子機構の理解　　　　　　　　　　　　　　　
Oct. 2023 - Sep. 2025

Temporal Factorsが神経の運命を決定する分子基盤の理解　　　　　　　　　　　　　　　
基盤研究（C）
Apr. 2021 - Mar. 2024

神経幹細胞が多種多様な神経細胞を作り分ける分子メカニズムの解明　　　　　　　　　　　　　　　
Apr. 2021 - Mar. 2024

神経幹細胞が多様な神経を生み出す分子メカニズムの理解　　　　　　　　　　　　　　　
Dec. 2021 - Aug. 2023

神経回路の多様性を飛躍的に増加させる分子メカニズム　　　　　　　　　　　　　　　
Aug. 2022 - Mar. 2023

神経産生期からグリア産生期への切り替えスイッチをONにする神経由来の生理活性物質の探索　　　　　　　　　　　　　　　
Apr. 2022 - Mar. 2023

ゲノムDNA上の疾病原因領域を迅速に特定する新規技術の開発　　　　　　　　　　　　　　　
Mar. 2022 - Mar. 2023

ゲノムDNAの3次元的な高次構造情報を任意の細胞で取得する新規手法の開発　　　　　　　　　　　　　　　
Jan. 2022 - Mar. 2023

迅速にエンハンサーを同定し疾病原因領域を特定する技術の開発　　　　　　　　　　　　　　　
Dec. 2021 - Mar. 2023

神経幹細胞が多種多様な神経細胞を作り分ける分子メカニズムの解明　　　　　　　　　　　　　　　
Apr. 2021 - Mar. 2022

Understanding of the molecular mechanisms that regulate production of neuronal diversity　　　　　　　　　　　　　　　
Apr. 2021 - Mar. 2022

遺伝子の発現調節領域の同定により疾病の原因箇所を迅速に特定する新規技術の開発　　　　　　　　　　　　　　　
Apr. 2021 - Mar. 2022

多様な神経を必要な数だけ生み出すメカニズムを解明する　　　　　　　　　　　　　　　
Dec. 2020 - Mar. 2022

神経幹細胞が多種多様な神経を過不足なく生み出す分子メカニズムの解明　　　　　　　　　　　　　　　
Nov. 2020 - Mar. 2022

新規手法DamIDによる神経多様性創出機構の解明　　　　　　　　　　　　　　　
Jan. 2020 - Apr. 2021

神経細胞の多様性を生み出す分子機構の解明　　　　　　　　　　　　　　　
Apr. 2020 - Mar. 2021

新たな手法DamIDを用いた多様な神経細胞を作り分けるメカニズムの解明　　　　　　　　　　　　　　　
研究活動スタート支援
Aug. 2019 - Mar. 2021

神経幹細胞の休眠制御による脳神経系修復メカニズムの解明　　　　　　　　　　　　　　　
Dec. 2019 - Nov. 2020

Understanding molecular mechanisms that regulate production of neuronal diversity by DamID, a newly established technique　　　　　　　　　　　　　　　
Oct. 2019 - Oct. 2020

神経多様性を創出する分子機構の解明　　　　　　　　　　　　　　　
Aug. 2019 - Jul. 2020

Identification and functional analysis of motion detection circuits
Grant-in-Aid for Scientific Research (B)
Kanazawa University
01 Apr. 2012 - 31 Mar. 2016