Daisuke SatoAssistant Professor
■Researcher basic information
Organization
Research Keyword
Educational Background
- Apr. 2014 - Sep. 2017, Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Department of Life Science and Systems Engineering
- Apr. 2012 - Mar. 2014, Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Department of Biological Functions and Engineering
- Apr. 2008 - Mar. 2012, Kyushu Institute of Technology, School of Computer Science and Systems Engineering, Department of Bioscience and Bioinformatics
- Apr. 2004 - Mar. 2007, Fukuoka Prefectural Tochiku Senior High School, General Course
Career
- Apr. 2025, Ibaraki University, Graduate School of Science and Engineering, Assistant Professor
- Apr. 2023 - Mar. 2025, Yokohama National University, Faculty of Engineering Division of Materials Science and Chemical Engineering, Postdoctoral Fellow
- Oct. 2022 - Mar. 2023, Kyushu University, Graduate School of Pharmaceutical Sciences, Special Researcher
- Apr. 2022 - Mar. 2023, Fukuoka Institute of Technology, Faculty of Engineering Department of Life, Environment and Applied Chemistry, Part-time Lecturer
- Apr. 2021 - Sep. 2022, Kyushu University, Graduate School of Pharmaceutical Sciences, Postdoctoral Fellow
- Feb. 2019 - Feb. 2021, North Carolina State University, Department of Chemistry, Research Scholar
- Oct. 2017 - Feb. 2019, Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Industry-Government-Academia Collaboration
External link
■Research activity information
Paper
- Total synthesis of antifungal cyclic hexapeptide ASP2397 and its derivatives
Daisuke Sato; Izuru Kawamura, Lead
Peptide Science 2023, Aug. 2024, [Reviewed] - In situ detection of enzymatic activities by the bioorthogonal reaction-coupled assay using a dialkoxysilylated fluorogenic probe
Daisuke Sato; Yuya Matsuo; Yuki Nishime; Shohei Uchinomiya; Akio Ojida, Lead, Abstract
Fluorescent probes are indispensable tools for detection of enzymatic activities. However, detection of enzymes with high substrate specificity with substrate-based fluorescent probes is still challenging, due to their relatively large molecular size. We report herein enzyme assays coupled with a bioorthogonal fluoride ion-induced desilylation of fluorogenic probe. This sensing system is useful for in situ detection of enzyme reactions with high substrate specificity., Oxford University Press (OUP)
Chemistry Letters, 01 Mar. 2024, [Reviewed] - Investigation of amylose and tailored amylose matrices for scavenging iodide
Jinghuai Dou; Daisuke Sato; Juno Son; Qihui Liu; Jonathan S. Lindsey, Lead, SPIE
Molecular and Nanophotonic Machines VI, 05 Oct. 2023 - Indoxyl-glucosides bearing tethers for enzymatically triggered cross-linking
Daisuke Sato; Zhiyuan Wu; Jinghuai Dou; Juno Son; Jonathan S. Lindsey, Lead, Tethered indoxyl-glucosides upon treatment with β-glucosidase under physiological conditions afford the corresponding indigoid dye via oxidative dimerization., Royal Society of Chemistry (RSC)
New Journal of Chemistry, 2023, [Reviewed] - Design, Synthesis, and Photo-Responsive Properties of a Collagen Model Peptide Bearing an Azobenzene
Daisuke Sato; Hitomi Goto; Yui Ishizaki; Tetsuya Narimatsu; Tamaki Kato, Lead, Collagen is a vital component of the extracellular matrix in animals. Collagen forms a characteristic triple helical structure and plays a key role in supporting connective tissues and cell adhesion. The ability to control the collagen triple helix structure is useful for medical and conformational studies because the physicochemical properties of the collagen rely on its conformation. Although some photo-controllable collagen model peptides (CMPs) have been reported, satisfactory photo-control has not yet been achieved. To achieve this objective, detailed investigation of the isomerization behavior of the azobenzene moiety in CMPs is required. Herein, two CMPs were attached via an azobenzene linker to control collagen triple helix formation by light irradiation. Azo-(PPG)10 with two (Pro-Pro-Gly)10 CMPs linked via a photo-responsive azobenzene moiety was designed and synthesized. Conformational changes were evaluated by circular dichroism and the cis-to-trans isomerization rate calculated from the absorption of the azobenzene moiety indicated that the collagen triple helix structure was partially disrupted by isomerization of the internal azobenzene., MDPI AG
Organics, 11 Oct. 2022, [Reviewed] - Design, Synthesis, and Utility of Defined Molecular Scaffolds
Daisuke Sato; Zhiyuan Wu; Hikaru Fujita; Jonathan Lindsey, Lead, A growing theme in chemistry is the joining of multiple organic molecular building blocks to create functional molecules. Diverse derivatizable structures—here termed “scaffolds” comprised of “hubs”—provide the foundation for systematic covalent organization of a rich variety of building blocks. This review encompasses 30 tri- or tetra-armed molecular hubs (e.g., triazine, lysine, arenes, dyes) that are used directly or in combination to give linear, cyclic, or branched scaffolds. Each scaffold is categorized by graph theory into one of 31 trees to express the molecular connectivity and overall architecture. Rational chemistry with exacting numbers of derivatizable sites is emphasized. The incorporation of water-solubilization motifs, robust or self-immolative linkers, enzymatically cleavable groups and functional appendages affords immense (and often late-stage) diversification of the scaffolds. Altogether, 107 target molecules are reviewed along with 19 syntheses to illustrate the distinctive chemistries for creating and derivatizing scaffolds. The review covers the history of the field up through 2020, briefly touching on statistically derivatized carriers employed in immunology as counterpoints to the rationally assembled and derivatized scaffolds here, although most citations are from the past two decades. The scaffolds are used widely in fields ranging from pure chemistry to artificial photosynthesis and biomedical sciences., MDPI AG
Organics, 11 Jul. 2021, [Reviewed] - Dual emissive bispyrene peptide probes for highly sensitive measurements of trypsin activity and evaluation of trypsin inhibitors
Daisuke Sato; Takuya Kondo; Tamaki Kato, Lead, Elsevier BV
Bioorganic & Medicinal Chemistry, Jul. 2018, [Reviewed] - Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye
Maryala Saikiran; Daisuke Sato; Shyam S. Pandey; Shuzi Hayase; Tamaki Kato, Elsevier BV
Bioorganic & Medicinal Chemistry Letters, Sep. 2017, [Reviewed] - Photophysical characterization and BSA interaction of the direct ring carboxy functionalized unsymmetrical NIR cyanine dyes
Maryala Saikiran; Daisuke Sato; Shyam S. Pandey; Takeshi Ohta; Shuzi Hayase; Tamaki Kato, Elsevier BV
Dyes and Pigments, May 2017, [Reviewed] - Novel fluorescent substrates for detection of trypsin activity and inhibitor screening by self-quenching
Daisuke Sato; Tamaki Kato, Lead, Elsevier BV
Bioorganic & Medicinal Chemistry Letters, Dec. 2016, [Reviewed] - Photophysical investigations of squaraine and cyanine dyes and their interaction with bovine serum albumin
M Saikiran; D Sato; S S Pandey; T Kato, IOP Publishing
Journal of Physics: Conference Series, 19 Apr. 2016, [Reviewed] - Detection of Protease Activity by Concentration Quenching-Based Substrates
Zhe Wu; Daisuke Sato; Tamaki Kato
Peptide Science 2013, Mar. 2014, [Reviewed] - Photocontrol of Collagen Triple Helix Self Assembly by Azobenzene Linker
Daisuke Sato; Hitomi Goto; Tamaki Kato, Lead
Peptide Science 2013, Mar. 2014, [Reviewed]
MISC
- Detection of Protease Activity Using Intramolecular Excimer Forming Bispyrene Peptide Substrates
Daisuke Sato
Journal of Peptide Science, 2016
Lead - Detection of Protease Activity by Concentration Quenching-Based Substrates
Daisuke Sato; Zhe Wu; Tamaki Kato
Peptides 2015, Proceedings of the 24th American Peptide Symposium, 2015
Lead
Lectures, oral presentations, etc.
- Sequence-Dependent Association States of β-Helix Peptides with Ion Channel Activity
Daisuke Sato; Haruto Nakajima; Soki Hou; Fumihiro Kayamori; Kenji Usui; Ai Niitsu; Ryuji Kawano; Izuru Kawamura
The Nanopore Meeting Tokyo 2025, 15 Mar. 2025
20250314, 20250315 - De novo-design, synthesis, and evaluation of amphiphilic β-helical peptide aimed to nanopore sensing application
佐藤 大輔; 中嶋 悠人; ホウ ソキ; 栢森 史浩; 臼井 健二; 川野 竜司; 川村 出
第61回ペプチド討論会, 29 Oct. 2024
20241029, 20241031 - Total synthesis of antifungal cyclic hexapeptide ASP2397 and its derivatives
佐藤 大輔; 加藤 珠樹; 川村 出
第60回ペプチド討論会, 08 Nov. 2023
20231108, 20231110 - "due to COVID-19" ー アメリカでのポスドク生活
佐藤 大輔
第96回日本生化学会大会, 01 Nov. 2023, [Invited]
20231031, 20231102 - 生物有機化学を基盤とするケミカルプローブおよび機能性分子の創製
佐藤 大輔
第6回ナノバイオ交流会（サイエンスライブチケット Part61）, 16 Jun. 2023, [Invited]
20230616, 20230616 - 生体直交的脱シリル化反応を用いたケミカルプローブの開発
佐藤 大輔; 内之宮 祥平; 王子田 明夫
日本化学会 第102春季年会, 24 Mar. 2022
20220323, 20220326 - Detection of protease activity by fluorescence alteration of pyrene using bispyrene peptide substrates
Daisuke Sato; Takuya Kondo; Tamaki Kato
The 25th American Peptide Symposium and 9th International Peptide Symposium, 19 Jun. 2017
20170617, 20170622 - 環状ペプチド中のD-アミノ酸
佐藤 大輔; 加藤 珠樹
生命の起原および進化学会 第42回学術講演会, 28 Mar. 2017
20170328, 20170330 - Detection of protease activity using intramolecular excimer forming bispyrene peptide substrates
Daisuke Sato; Takuya Kondo; Tamaki Kato
The 34th European Peptide Symposium and 8th International Peptide Symposium, 06 Sep. 2016
20160903, 20160909 - リシン分岐型濃度消光基質を利用したプロテアーゼ活性の検出
佐藤 大輔; 加藤 珠樹
日本化学会 第96春季年会, 27 Mar. 2016
20160324, 20160327 - Detection of trypsin activity by concentration quenching-based substrates
Daisuke Sato; Tamaki Kato
India-Japan Expert Group Meeting on Biomolecular Electronics and Organic Nanotechnology for Environment Preservation 2015, 23 Dec. 2015
20151222, 20151225 - Detection of thrombin activity using concentration quenching-based substrates
Daisuke Sato; Tamaki Kato
The 7th International Peptide Symposium, 10 Dec. 2015
20151209, 20151211 - Detection of protease activity by concentration quenching-based substrates
Daisuke Sato; Zhe Wu; Tamaki Kato
The 24th American Peptide Symposium, 22 Jun. 2015
20150620, 20150625 - 蛍光の濃度消光を利用したプロテアーゼ活性検出基質ペプチドの改良設計
佐藤 大輔; 武 哲; 加藤 珠樹
日本化学会 第95春季年会, 26 Mar. 2015
20150326, 20150329 - アゾベンゼンを分子内にもつ光応答性コラーゲンペプチドの創製
佐藤 大輔; 後藤 仁美; 加藤 珠樹
第63回高分子学会年次大会, 28 May 2014
20140528, 20140530 - Photocontrol of collagen triple helix self-assembly by azobenzene linker
Daisuke Sato; Hitomi Goto; Tamaki Kato
The 4th Asia-Pacific International Peptide Symposium and 50th Japanese Peptide Symposium, 07 Nov. 2013
20131106, 20131108 - Detection of protease activity by concentration quenching-based substrates
Daisuke Sato; Zhe Wu; Tamaki Kato
The 1st International Symposium on Applied Engineering and Sciences, 01 Oct. 2013
20130930, 20131001 - 蛍光性基質ペプチドの濃度消光を利用したプロテアーゼ活性の検出
佐藤 大輔; 服部 司; Ella Blanque; 加藤 珠樹
日本化学会 第93春季年会, 24 Mar. 2013
20130322, 20130325 - シンデンドリマーとFRET型蛍光性ペプチドを用いたプロテアーゼ活性検出蛍光性基質ペプチドの濃度消光を利用したプロテアーゼ活性の検出
佐藤 大輔; 服部 司; 加藤 珠樹
日本化学会 第92春季年会, 25 Mar. 2012
20120325, 20120328
Courses
Research Themes
- Total synthesis and application to structure-activity relationship studies of hydroxamate-type cyclic peptides CHAP108 and ASP2397
Grant-in-Aid for Early-Career Scientists
Yokohama National University
01 Apr. 2024 - 31 Mar. 2027
Industrial Property Rights
