Hiroyuki OhtaPresident

■Researcher basic information

Organization

  • President

Research Areas

  • Life sciences, Plant nutrition, soil science, Plant Nutrient Science/Soil Studies
  • Environmental science/Agricultural science, Environmental impact assessment, Environmental Influence Valuation/Environment Policy
  • Environmental science/Agricultural science, Environmental dynamics, Environmental Dynamic Analysis

Research Keyword

  • Microbial Ecology, Environmental Chemistry and Toxicology, Microbial Physiology, Bacterial Taxonomy

Degree

  • 1982年03月 農学博士(東北大学)

Educational Background

  • Apr. 1979 - Mar. 1983, Tohoku University, 農学研究科博士後期課程, 農芸化学

Career

  • Apr. 2020, 茨城大学, 学長
  • Apr. 2016 - Mar. 2020, Ibaraki University, Director, Vice President (Education)
  • Sep. 2014 - Mar. 2016, Ibaraki University, Vice President
  • Jun. 2002, Ibaraki University, College of Agriculture, Professor
  • Aug. 1997 - May 2002, Ibaraki University, College of Agriculture, Associtae Professor
  • Jun. 1992 - Jul. 1997, Okayama University, Dental School, Associtae Professor
  • Apr. 1984 - May 1992, Okayama University, Dental School, Assistant Professor
  • Apr. 1982 - Mar. 1984, Tohoku University, Institute for Agricultural Research, Postdoctoral fellow, Japan Promotion of Science

Member History

  • Apr. 2020 - Mar. 2022, 会長, 日本土壌肥料学会関東支部
  • Jun. 2017 - May 2021, President, Japanese Society of Soil Microbiology
  • Jun. 2007 - May 2013, 評議員, 日本土壌微生物学会
  • 2009 - 2013, International Committee, International Society for Environmental Biogeochemistry
  • Jan. 2010 - Dec. 2012, 評議員, Japanese Society for Soil Microbiology
  • Jan. 2005 - Dec. 2008, 評議員, Japanese Society for Soil Microbiology
  • Jan. 2005 - Dec. 2008, 学会誌編集委員, Japanese Society for Soil Microbiology
  • Jan. 2003 - Dec. 2004, 事務局代表幹事, Japanese Society for Soil Microbiology
  • Jan. 2000 - Dec. 2003, 企画幹事, 日本土壌微生物学会
  • Apr. 1998 - Mar. 2001, 副部門長(第3部門), 日本土壌肥料学会
  • Apr. 1998 - Mar. 2001, 欧文誌編集委員, 日本土壌肥料学会

Message from Researchers

  • (Message from Researchers)

    (研究経歴)
    1982-1984 Engaged in research on the Soil Oligotrophic Bacteria 1984-1998 Engaged in research on Oral Bacteria 1998- Engaged in research on Nonylphenol Degrading Bacteria

■Research activity information

Award

  • Sep. 2016, Udayana Award, Udayana University, Indonesia
    Hiroyuki Ohta
    Others
  • Sep. 2014, 第59回日本土壌肥料学会賞, 土壌細菌の分類および群集解析に関する研究, 日本土壌肥料学会
  • Jul. 2013, 特別研究員等審査会専門委員(書面担当) 表彰, 日本学術振興会
    太田寛行
    Others
  • Nov. 2010, JSME Prize 2010 for the best publication, Nitrogenase activity (Acetylene Reduction) of an Iron-Oxidizing Leptospirillum Strain Cultured as a Pioneer Microbe from a Recent Volcanic Deposit on Miyake-jima, Japan, 日本微生物生態学会
    Yoshinori Sato;Ken Hosokawa;Reiko Fujimura;Tomoyasu Nishizawa;Takashi Kamijo;Hiroyuki Ohta
    Official journal
  • 1993, 両備(]J3727[)園記念財団研究助成

Paper

  • 〔Major achievements〕Plant growth promoting potential of Arthrobacter spp., an early colonizer on the volcanic deposits of Mt. Merapi, Indonesia, as a restorative agent for degraded land
    Annisa Nur Lathifah; Irfan Dwidya Prijambada and Hiroyuki Ohta, Last, Exploration of novel and effective plant growth-promoting bacteria (PGPB) from unexplored environments is an intriguing option for restoring degraded land in Indonesia caused by mining activity. Due to their ability to develop in the absence of carbon, PGPB derived from volcanic environments may be a sustainable alternative. Previous research on the volcanic deposits of Mt. Merapi, Indonesia and Miyake-Jima, Japan, revealed that Arthrobacter spp. dominated the pioneer bacterial communities. Consequently, this research aimed to investigate the biochemical and physiological characteristics of volcanic Arthrobacter groups and assess their potential as PGPB., EDP Sciences
    E3S Web of Conferences, 02 Feb. 2024, [Reviewed]
  • 〔Major achievements〕Tardiphaga alba sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil
    Zhihua Bao; Chaojun Wang1; Jiahui Cao; Tingting Zhang; Yong Guo; Yoshinori Sato; Tomoyasu Nishizawa; Hiroyuki Ohta, Last, A previously undescribed, heavy-metal-tolerant, motile, Gram-negative bacterium, designated strain SK50-23T, was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK50-23T was closely related to Tardiphaga robiniae LMG 26467T and the non-phototrophic ‘Rhodopseudomonas boonkerdii’ NS23T (98.1 and 97.3 % 16S rRNA gene sequence similarity, respectively). Strain SK50-23T could be assigned to the genus Tardiphaga with the species name Tardiphaga alba sp. nov. based on morphological, chemotaxonomic and genome-based taxonomic characteristics, and 16S rRNA gene-based phylogenetic characteristics., Microbiology Society
    International Journal of Systematic and Evolutionary Microbiology, 10 Jan. 2024, [Reviewed]
  • Dark septate endophytic fungi associated with pioneer grass inhabiting volcanic deposits and their functions in promoting,plant growth
    Han Sun; Tomoyasu Nishizawa; Hiroyuki Ohta; and Kazuhiko Narisawa, Growth of the pioneer grass Miscanthus condensatus, one of the first vegetation types to be established on volcanic deposits, is promoted by root-associated fungi, particularly dark septate endophytes (DSEs). Fungal taxa within DSEs colonize the root of Miscanthus condensatus in oligotrophic Andosol, and their function in plant growth promotion remains largely unknown. We, therefore, comprehensively assessed the composition of the DSE community associated with Miscanthus condensatus root in volcanic ecosystems using the approaches of both metabarcoding (next-generation sequencing) and isolation (culturing).
    Biogeosciences, 29 Nov. 2023, [Reviewed]
  • Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales
    Yusuke Takashima; Kohei Yamamoto; Yousuke Degawa; Yong Guo; Tomoyasu Nishizawa; Hiroyuki Ohta and Kazuhiko Narisawa, Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found ,and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded ,in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. ,Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island ,in the Ogasawara (Bonin) Islands, Japan., Springer Nature
    IMA Fungus, 23 Nov. 2023, [Reviewed]
  • 〔Major achievements〕A Nitrate-Transforming Bacterial Community Dominates in the Miscanthus Rhizosphere on Nitrogen-Deficient Volcanic Deposits of Miyake-jima
    Ahmad Arsyadi; Yong Guo; Akiko Ebihara; Nobuo Sakagami; Midori Sakoda; Kanako Tago; Takashi Kamijo; Hiroyuki Ohta and Tomoyasu Nishizawa, The perennial gramineous grass Miscanthus condensatus functions as a major pioneer plant in colonizing acidic volcanic deposits on Miyake-jima, Japan, despite a lack of nitrogen nutrients. The nitrogen cycle in the rhizosphere is important for the vigorous growth of M. condensatus in this unfavorable environment. In the present study, we identified the nitrogen-cycling bacterial community in the M. condensatus rhizosphere on these volcanic deposits using a combination of metagenomics and culture-based analyses. Our results showed a large number of functional genes related to denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in the rhizosphere, indicating that nitrate-transforming bacteria dominated the rhizosphere biome., MDPI
    Microorganisms, 19 Jan. 2023, [Reviewed]
  • Whole genome sequence of Entomortierella parvispora E1425, a Mucoromycotan fungus associated with Burkholderiaceae-related endosymbiotic bacteria
    Afri Herlambang; Yong Guo; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta; and Tomoyasu Nishizawa, Some mucoromycotan fungi establish symbiotic associations with endohyphal bacteria. Herein, the genome of Entomortierella parvispora E1425 (synonymously known as Mortierella parvispora E1425), which harbors a cultured Burkholderiaceae-related endobacterium (BRE) designated as Mycoavidus sp. B2-EB, was sequenced. In this report, we provide genomic information to elucidate fungal-BRE symbiotic features., American Society for Microbiology
    Microbiology Resource Announcements, 13 Jan. 2022, [Reviewed]
  • Complete genome sequence of a chemolithoautotrophic iron-oxidizing bacterium Acidithiobacillus ferrooxidans strain NFP31, isolated from volcanic ash deposits on Miyake-jima, Japan
    Tatsuhiro Katoa; Yong Guo; Reiko Fujimura; Takamichi Nakamura; Tomoyasu Nishizawa; Yasuro Kurusub; Hirouki Ohta, Last, The genome sequence of Acidithiobacillus ferrooxidans strain NFP31, which is a chemolithoautotrophic iron-oxidizing bacterium that inhabits acidified volcanic deposits on Mount Oyama, Miyake island (Miyake-jima), Japan, was determined to identify the genetic characteristics associated with pioneer microbes in newly placed pyroclastic deposits., American Society for Microbiology
    Microbiology Resource Announcements, 13 Jan. 2022, [Reviewed]
  • Root bacteriome of a pioneer grass Miscanthus condensatus along restored vegetation on recent Miyake-jima volcanic deposits
    Yong Guo; Tomoyasu Nishizawa; Nobuo Sakagami; Reiko Fujimura; Takashi Kamijo; Hiroyuki Ohta, Last, Root-associated microorganisms play an important role in colonization and subsequent development of pioneer plants on newly exposed substrates such as volcanic deposits. However, there is still lack of an understanding how the root microbiome establishes during a short-term development without apparent soil formation when the pioneer plant colonizes on the pristine substrates. Miscanthus condensatus, the first grass to colonize recent volcanic deposits on the Island of Miyake (Miyake-jima), Japan, is a model plant suitable to address this issue. In this study, we carried out bacterial 16S rRNA genes real-time quantitative PCR, T-RFLP profiling and clone library analyses to assess the root bacteriome of M. condensatus from two phases of different plant-colonization, i.e., early colonizing phase (sites OY and IG7) and later dominating phase (sites IG8 and IG9)., Elsevier B.V.
    Rhizosphere, 13 Aug. 2021, [Reviewed]
  • Soil microbes and climate change               
    Hiroyuki Ohta; Tomoyasu Nishizawa, Lead, 9カ国33名の微生物学者によって合意声明の論文「人類への注意喚起:微生物と気候変動」が発表された(2019年6月)。本総説は、その合意声明を受けて、土壌からの温室効果ガス発生の概要、土壌有機物の存在状態と微生物との関わり、土壌中での一酸化二窒素(N2O)の生成と消費のプロセス、土壌の環境条件とN2O生成の関係、そして土壌からのN2O発生の制御に向けて、最近の知見を整理した。, Japanese Society of Soil Microbiology
    Soil microorganisms, Jun. 2021, [Reviewed], [Invited]
  • Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional,Development on O‐yama Volcano, Miyake‐jima, Japan
    Amber N. DePoy; Gary M. King; and Hiroyuki Ohta, Last, Research on Kilauea and O‐yama Volcanoes has shown that microbial communities and,their activities undergo major shifts in response to plant colonization and that molybdenum‐dependent CO oxidizers (Mo‐COX) and their activities vary with vegetation and deposit age. Results reported here reveal that anaerobic CO oxidation attributed to nickel‐dependent CO,oxidizers (Ni‐COX) also occurs in volcanic deposits that encompass different developmental stages. Ni‐COX at three distinct sites responded rapidly to anoxia and oxidized CO from initial concentrations of about 10 ppm to sub‐atmospheric levels. CO was also actively consumed at initial,25% concentrations and 25 °C, and during incubations at 60 °C; however, uptake under the latter conditions was largely confined to an 800‐year‐old forested site., MDPI
    Microorganisms, Jan. 2021, [Reviewed]
  • Aggregate distribution and substrate-induced respiration under different tillage and mulching management systems in organic farming
    Rahmatullah Hashimi; Satoshi Kaneda; Nobuhiro Kaneko; Hiroyuki Ohta &; Masakazu Komatsuzaki, In organic farming, intensive tillage for weed eliminating may cause physical and biological soil degradation. Weed residue mulch can be used to stabilize the organic carbon in the soil by improving soil,aggregation and increasing microbial activity. However, the effectiveness of weed mulch in no-tillage (NT) and conventional tillage (CT) systems remains unclear. Therefore, we studied the influence of clipped weed mulch (CM) and no mulch (NM), in combination with NT and CT on the water-stable aggregates, and carbon stabilization in different-sized soil aggregates as well as on substrate-induced respiration (SIR) and the fungal-to-bacterial activity ratio at a soil depth of 0–5 cm., Tayler & Francis
    Soil Science and Plant Nutrition, 12 Oct. 2020, [Reviewed]
  • Mycoavidus sp. strain B2-EB: Comparative genomics reveals minimal genomic features required by a cultivable Burkholderiaceae-related endofungal bacterium
    Yong Guo; Yusuke Takashima; a Yoshinori Sato; Kazuhiko Narisawa; Hiroyuki Ohta; Tomoyasu Nishizawa, Obligate bacterial endosymbionts are critical to the existence of many eukaryotes. Such endobacteria are usually characterized by reduced genomes and metabolic dependence on the host, which may cause difficulty in isolating them in pure cultures. Family Burkholderiaceae-related endofungal bacteria affiliated with the Mycoavidus-Glomeribacter clade can be associated with the fungal subphyla Mortierellomycotina and Glomeromycotina. In this study, a cultivable endosymbiotic bacterium, Mycoavidus sp. strain B2-EB, present in the fungal host Mortierella parvispora was obtained successfully. T, American Society for Microbiology
    Applied and Environmental Microbiology, 10 Jul. 2020, [Reviewed]
  • Enumeration of Chemoorganotrophic Carbonyl Sulfide (COS)-degrading Microorganisms by the Most Probable Number Method
    Hiromi Kato; Takahiro Ogawa; Hiroyuki Ohta; Yoko Katayama, Carbonyl sulfide (COS) is the most abundant sulfur compound in the atmosphere, and, thus, is important in the global sulfur cycle. Soil is a major sink of atmospheric COS and the numerical distribution of soil microorganisms that degrade COS is indispensable for estimating the COS-degrading potential of soil. However, difficulties are associated with counting COS-degrading microorganisms using culture-dependent approaches, such as the most probable number (MPN) method, because of the chemical hydrolysis of COS by water. We herein developed a two-step MPN method for COS-degrading microorganisms: the first step for chemoorganotrophic growth that supported a sufficient number of cells for COS degradation in the second step., Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology,Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
    Microbes and Environments, 29 Apr. 2020, [Reviewed]
  • Aposymbiosis of a Burkholderiaceae-Related Endobacterium Impacts on Sexual Reproduction of Its Fungal Host
    Yusuke Takashima; Yousuke Degawa; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Bacterial endosymbionts inhabit diverse fungal lineages. Although the number of studies on bacteria is increasing, the mechanisms by which bacteria affect their fungal hosts remain unclear. We herein examined the homothallic isolate, Mortierella sugadairana YTM39, harboring a Burkholderiaceae-related endobacterium, which did not produce sexual spores. We successfully eliminated the bacterium from fungal isolates using ciprofloxacin treatment and asexual spore isolation for germinated asexual spores., Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology,Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
    Microbes and Environments, 15 Apr. 2020, [Reviewed]
  • Comparative characterization of bacterial communities in moss-covered and unvegetated volcanic deposits of Mount Merapi, Indonesia
    Annisa N. Lathifah; Yong Guo; Nobuo Sakagami; Wataru Suda; Masanobu Higuchi; Tomoyasu Nishizawa; Irfan D. Prijambada; Hiroyuki Ohta, Corresponding, Microbial colonization, followed by succession, on newly exposed volcanic substrates represents the beginning of an early ecosystem development. During early succession, colonization by mosses or plants significantly alters the pioneer microbial community composition through the photosynthetic carbon input. To provide more insight into this process, we investigated the three-year-old volcanic deposits of Mount Merapi, Indonesia., Japan Society of Microbial Ecology
    Microbes and Environments, 25 Sep. 2019, [Reviewed]
  • Phylogenetic characterization of viable but-not-yet cultured Legionella groups grown in amoebic cocultures: a case study using various cooling tower water samples               
    Hiroaki Inoue; Kunio Agata; Hiroyuki Ohta, Legionella spp. exist naturally in association with amoeba in water environments and are known to be the etiological agent of a severe form of pneumonia. To detect diverse Legionella populations in cooling tower water systems, the amoebic coculturing was performed for 15 water samples obtained form five different kind facilities in six geographically different locations., The Society for Antibacterial and Antifungal Agents, Japan
    Biocontrol Science, 18 Mar. 2019, [Reviewed]
  • Comparative analysis of the genetic basis of branched nonylphenol degradation by Sphingobium amiense DSM 16289T and Sphingobium cloacae JCM 10874T
    Mina Ootsuka; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta, Corresponding, Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae JCM 10874T.
    Microbes and Environments, 28 Dec. 2018, [Reviewed]
  • Prevalence and intra-family phylogenetic divergence of Burkholderiaceae-related endobacteria associated with species of Mortierella
    Takashima, Y; K. Seto; Y. Degawa; Y. Guo; T. Nishizawa; H. Ohta; K. Narisawa, JSME, JSSM
    Microbes Environ., Dec. 2018, [Reviewed]
  • Complete genome sequence of Agrobacterium pusense VsBac-Y9, a,bacterial symbiont of the dark septate endophytic fungus Veronaeopsis,simplex Y34 with potential for improving fungal colonization in roots               
    Yong Guo; Yuuto Matsuoka; Takamasa Miura; Tomoyasu; Nishizawa; Hiroyuki Ohta; Kazuhiko Narisa, A Rhizobium-related bacterium (Rhizobium sp. VsBac-Y9) is a,symbiont living with the dark septate endophytic (DSE) fungus,Veronaeopsis simplex Y34. Co-inoculation of Rhizobium sp. VsBac-Y9 with,V. simplex Y34 improves the fungal colonization of tomato roots,,resulting in a significant increase in aboveground biomass. This study,sequenced the complete genome of this V. simplex-helper bacterium using,the PacBio and Illumina MiSeq platforms.
    Journal of Biotechnology, 02 Aug. 2018, [Reviewed]
  • Mortierella sugadairana, a new homothallic species related to the firstly described heterothallic species in the genus
    Yusuke Takashima; Yousuke Degawa; Hiroyuki Ohta; Kazuhiko Narisawa, A new species, Mortierella sugadairana, is described for a fungus forming homothallic zygospores with a club-shaped macrosuspensor and a microsuspensor originating from the macrosuspensor. The species was isolated from cool regions in Japan and morphologically and phylogenetically close to a heterothallic species M. parvispora, which is the first species reported as a heterothallic species in the genus. Mycelial growth of the species was limited at 30 °C, whereas two isolates of M. parvispora can grow. This may indicate that the species and M. parvispora adapted to different climates from a common ancestor involving differentiation of the manner of reproduction., Elsevier B.V.
    Mycoscience, 01 May 2018, [Reviewed]
  • Effects of Rhizobium Species Living with the Dark Septate Endophytic Fungus Veronaeopsis simplex on Organic Substrate Utilization by the Host
    Yong Guo; Yuuto Matsuoka; Tomoyasu Nishizawa; Hiroyuki Ohta; Kazuhiko Narisawa, Bacteria harbored in/on the hyphae of the dark septate endophyte, Veronaeopsis simplex Y34, were identified as a single Rhizobium species by molecular analyses of bacterial 16S rRNA genes, and were successfully isolated from the endophyte., Japanese Society of Microbial Ecology
    Microbes and Environments, 2018, [Reviewed]
  • Comparative Genomic Insights into Endofungal Lifestyles of Two Bacterial Endosymbionts, Mycoavidus cysteinexigens and Burkholderia rhizoxinica
    Dilruba Sharmin; Yong Guo; Tomoyasu Nishizawa; Shoko Ohshima; Yoshinori Sato; Yusuke Takashima; Kazuhiko Narisawa; Hiroyuki Ohta, Endohyphal bacteria (EHB), dwelling within fungal hyphae, markedly affect the growth and metabolic potential of their hosts. To date, two EHB belonging to the family Burkholderiaceae have been isolated and characterized as new taxa, Burkholderia rhizoxinica (HKI 454T) and Mycoavidus cysteinexigens (B1-EBT), in Japan. Metagenome sequencing was recently reported for Mortierella elongata AG77 together with its endosymbiont M. cysteinexigens (Mc-AG77) from a soil/litter sample in the USA. In the present study, we elucidated the complete genome sequence of B1-EBT and compared it with those of Mc-AG77 and HKI 454T., Japanese Society of Microbial Ecology
    Microbes and Environments, 2018, [Reviewed]
  • Draft genome sequences of Aggregatibacter actinomycetemcomitans strains 310a and 310b
    Anthony C. May; Hiroyuki Ohta; Hiroshi Maeda; Susumu Kokeguchi; Carla Cugini, We report the draft genome sequences of Aggregatibacter actinomycetemcomitans strains 310a (310-TR) and 310b (310-OS). Strain 310a is a clinical isolate with a rough phenotype. Strain 310b is a laboratory-adapted isolate derived from the passage of 310a and displays a smooth phenotype., American Society for Microbiology
    Genome Announcements, 01 Nov. 2017, [Reviewed]
  • Complete Genome Sequence of the Nonylphenol-Degrading Bacterium Sphingobium cloacae JCM 10874T
    Mina Ootsukaa; Tomoyasu Nishizawaa; Hiroyuki Ohta, Corresponding, Sphingobium cloacae JCM 10874T can degrade phenolic endocrine-disrupting chemicals, nonylphenol, and octylphenol. Here, we report the complete genome sequence of the JCM 10874T strain., American Society for Microbiology
    Genome Announcements, Nov. 2016, [Reviewed]
  • Bacterial communities in different locations, seasons and segments of a dairy wastewater treatment system consisting of six segments
    Kikue Hirota; Yuji Yokota; Toru Sekimura; Hiroshi Uchiumi; Yong Guo; Hiroyuki Ohta; Isao Yumoto, A dairy wastewater treatment system composed of the 1st segment (no aeration) equipped with a facility for the destruction of milk fat particles, four successive aerobic treatment segments with activated sludge and a final sludge settlement segment was developed. The activated sludge is circulated through the six segments by settling sediments (activated sludge) in the 6th segment and sending the sediments beck to the 1st and 2nd segments. Microbiota was examined using samples from the non-aerated 1st and aerated 2nd segments obtained from two farms using the same system in summer or winter. Principal component analysis showed that the change in microbiota from the 1st to 2nd segments concomitant with effective wastewater treatment is affected by the concentrations of activated sludge and organic matter (biological oxygen demand [BOD]), and dissolved oxygen (DO) content. Microbiota from five segments (1st and four successive aerobic segments) in one location was also examined. Although the activated sludge is circulating throughout all the segments, microbiota fluctuation was observed. The observed successive changes in microbiota reflected the changes in the concentrations of organic matter and other physicochemical conditions (such as DO), suggesting that the microbiota is flexibly changeable depending on the environmental condition in the segments. The genera Dechloromonas, Zoogloea and Leptothrix are frequently observed in this wastewater treatment system throughout the analyses of microbiota in this study. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V., SCIENCE PRESS
    JOURNAL OF ENVIRONMENTAL SCIENCES, Aug. 2016, [Reviewed]
  • Efficient butanol recovery from acetone-butanol-ethanol fermentation cultures grown on sweet sorghum juice by pervaporation using silicalite-1 membrane
    Miho Kanemoto; Hideyuki Negishi; Keiji Sakaki; Toru Ikegami; Shigeru Chohnan; Youji Nitta; Yasurou Kurusu; Hiroyuki Ohta, Corresponding, We investigated butanol recovery by pervaporation separation, using a silicalite-1 membrane, from batch cultures of butanol-producing Clostridium beijerincicii SBP2 grown on sweet sorghum juice as a fermentation medium. The pervaporation system yielded 73% (w/v) butanol from intact feed cultures containing 1% (w/v) butanol, and had a butanol permeation flux of 11 g m(-2) h(-1). Upon neutralization and activated charcoal treatment of the feed cultures, butanol yield and total flux increased to 82% (w/v) and 40 g m(-2) h(-1), respectively. This system is applicable to refining processes for practical biobutanol production from a promising energy crop, sweet sorghum. (C) 2015 The Society for Biotechnology, Japan. All rights reserved., SOC BIOSCIENCE BIOENGINEERING JAPAN
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Jun. 2016, [Reviewed]
  • Mycoavidus cysteinexigens gen. nov., sp nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata
    Shoko Ohshima; Yoshinori Sato; Reiko Fujimura; Yusuke Takashima; Moriyuki Hamada; Tomoyasu Nishizawa; Kazuhiko Narisawa; Hiroyuki Ohta, Corresponding, An endohyphal bacterium (strain B1-EBT) living in association with the fungus Mortierella elongata FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EBT required cysteine for growth and grew at temperatures between 4 and 35 degrees C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EBT forms a distinct clade in the family Burkholderiaceae, encompassing a group of endosymbionts associated with several soil isolates of M. elongata. The most closely related genus is 'Candidatus Glomeribacter gigasporarum', an endosymbiont of the arbuscular mycorrhizal fungus Gigaspora margarita. The major cellular fatty acids of strain B1-EBT were C-16 : 0, summed feature 3 (C-16 : (1)omega 7c and C-16 : (1)omega 6c) and summed feature 8 (C-18 : (1)omega 7c or C-18 : (1)omega 6c). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EBT represents a novel genus and novel species in the family Burkholderiaceae, for which the name Mycoavidus cysteinexigens gen. nov., sp. nov. is proposed. The type strain is B1-EBT (=JCM 30646(T) =LMG 28693(T) =NBRC 110909(T))., SOC GENERAL MICROBIOLOGY
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, May 2016, [Reviewed]
  • Unique pioneer microbial communities exposed to volcanic sulfur dioxide
    Reiko Fujimura; Seok-Won Kim; Yoshinori Sato; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta, Corresponding, Newly exposed volcanic substrates contain negligible amounts of organic materials. Heterotrophic organisms in newly formed ecosystems require bioavailable carbon and nitrogen that are provided from CO2 and N-2 fixation by pioneer microbes. However, the knowledge of initial ecosystem developmental mechanisms, especially the association between microbial succession and environmental change, is still limited. This study reports the unique process of microbial succession in fresh basaltic ash, which was affected by long-term exposure to volcanic sulfur dioxide (SO2). Here we compared the microbial ecosystems among deposits affected by SO2 exposure at different levels. The results of metagenomic analysis suggested the importance of autotrophic iron-oxidizing bacteria, particularly those involved in CO2 and N-2 fixation, in the heavily SO2 affected site. Changes in the chemical properties of the deposits after the decline of the SO2 impact led to an apparent decrease in the iron-oxidizer abundance and a possible shift in the microbial community structure. Furthermore, the community structure of the deposits that had experienced lower SO2 gas levels showed higher similarity with that of the control forest soil. Our results implied that the effect of SO2 exposure exerted a selective pressure on the pioneer community structure by changing the surrounding environment of the microbes., NATURE PUBLISHING GROUP
    SCIENTIFIC REPORTS, Jan. 2016, [Reviewed]
  • Complete genome sequence of Streptomyces parvulus 2297, integrating sitespecifically with actinophage R4
    Tomoyasu Nishizawa; Takamasa Miura; Chizuko Harada; Yong Guo; Kazuhiko Narisawa; Hiroyuki Ohta; Hideo Takahashi; Makoto Shirai, Streptomyces parvulus 2297, which is a host for site-specific recombination according to actinophage R4, is derived from the type strain ATCC 12434. Species of S. parvulus are known as producers of polypeptide antibiotic actinomycins and have been considered for industrial applications. We herein report for the first time the complete genome sequence of S. parvulus 2297., American Society for Microbiology
    Genome Announcements, 2016, [Reviewed]
  • 岐阜県御嶽山の森林土壌から採取した菌核の培養法および非培養法による菌類群集解析               
    野々山; 佐々木; 弥; 坂上伸生; 成澤才彦; 太田寛行; 渡邊眞紀子, 岐阜県御嶽山の土壌から採取した菌核において,菌核形成菌を推定するためにクローンライブラリー法および菌類の分離をおこなった。クローンライブラリー法により,菌核形成菌として報告されているCenococcum geophilum が認められた。分離された糸状菌は,その大部分が子囊菌類であった。培養法ではC. geophilumが認められなかったものの, DSE に類別されると考えられる未同定菌3 株が,人工培地上で菌核を形成した。本研究で供試した菌核は, C. geophilum だけでなく,他の菌種により形成された可能性もあると考えられた。, 日本土壌微生物学会
    土と微生物(Soil Microorganisms), 2016, [Reviewed]
  • Responses of soil nematode community structure to soil carbon changes due to different tillage and cover crop management practices over a nine-year period in Kanto, Japan
    Ito; T.; M. Araki; T. Higashi; M. Komatsuzaki; N. Kaneko; H. Ohta, Last, The response of the soil food web structure to soil quality changes during long-term anthropogenic disturbance due to farming practices has not been well studied. We evaluated the effects of three tillage systems: moldboard plow/rotary harrow (MP), rotary cultivator (RC), and no-tillage (NT), three winter cover-crop types (fallow, FL; rye, RY; and hairy vetch, HV), and two nitrogen fertilization rates (0 and 100 kg N ha−1 for upland rice, and 0 and 20 kg N ha−1 for soybean production) on changes in nematode community structure., Elsevior
    Applied Soil Ecology, May 2015, [Reviewed]
  • Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
    Hiroaki Inoue; Reiko Fujimura; Kunio Agata; Hiroyuki Ohta, Last, Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    MICROBES AND ENVIRONMENTS, 2015, [Reviewed]
  • Soil nematode community structure affected by tillage systems and cover crop managements in organic soybean production.
    Ito; T.; M. Araki; M. Komatsuzaki; N. Kaneko; H. Ohta, The response of the soil food web structure to soil quality changes during long-term anthropogenic disturbance due to farming practices has not been well studied. We evaluated the effects of three tillage systems: moldboard plow/rotary harrow (MP), rotary cultivator (RC), and no-tillage (NT), three winter cover-crop types (fallow, FL; rye, RY; and hairy vetch, HV), and two nitrogen fertilization rates (0 and 100 kg N ha−1 for upland rice, and 0 and 20 kg N ha−1 for soybean production) on changes in nematode community structure., ELSEVIER SCIENCE BV
    Applied Soil Ecology, 2015, [Reviewed]
  • Sphingomonas oligoaromativorans sp. nov., an oligotrophic bacterium isolated from a forest soil
    Song-Ih Han; Jae-Chan Lee; Hiroyuki Ohta; Kyung-Sook Whang, A halo- and organo-sensitive oligotrophic bacterium, designated strain SY-6(T), was isolated from humus forest soil at Gyeryong mountain in Korea. Cells of the strain were Gram-negative, strictly aerobic, non-motile rods and the strain formed yellow-pigmented colonies on 100-fold-diluted nutrient broth. Strain SY-6(T) grew at pH 6.0-7.0 (optimal growth at pH 7.0), at 10-37 degrees C (optimal growth at 28 degrees C) and at salinities of 0-0.5 % (w/v) NaCl, growing optimally at 0.01% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain SY-6(T) was shown to belong to the genus Sphingomonas and showed the closest phylogenetic similarity to Sphingomonas polyaromaticivorans B2-7(T) (96.7%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine were Q-10 and sym-homospermidine, respectively. The major fatty acids were C-18:1 omega 7c and C-16:0. The DNA G+C content of the novel isolate was 65.3 mol%. On the basis of the evidence from this polyphasic study, strain SY-6T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas oligoaromativorans sp. nov. is proposed. The type strain is SY-6(T) (=KACC 12948(T)=NBRC 105508(T))., SOC GENERAL MICROBIOLOGY
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, May 2014, [Reviewed]
  • Tillage and cover crop species affect soil organic carbon in Andosol, Kanto, Japan               
    Tatsuya Higashi; Mu Yunghui; Masakazu Komatsuzaki; Shigenori Miura; Toshiyuki Hirata; Hajime Araki; Nobuhiro Kaneko; Hiroyuki Ohta, No-tillage, cover crops, and N fertilization play important roles in conserving or increasing soil organic carbon (SOC). However, the effects of their interaction are less well known, particularly in Asian countries. We examined the effects of three tillage management systems, moldboard plow/rotary harrow (MP), rotary cultivator (RC), and no-tillage (NT); three winter cover crop types (FL: fallow, RY: rye, and HV hairy vetch); and two nitrogen fertilization rates (0 and 100 kg N ha−1 for upland rice and 0 and 20 kg N ha−1 for soybean production) on changes in SOC.
    Soil & Tillage Research, Mar. 2014, [Reviewed]
  • Draft genome sequence of the betaproteobacterial endosymbiont associated with the fungus Mortierella elongata FMR23-6
    Reiko Fujimura; Ayumu Nishimura; Shoko Ohshima; Yoshinori Sato; Tomoyasu Nishizawa; Kenshiro Oshima; Masahira Hattori; Kazuhiko Narisawa; Hiroyuki Ohta, The fungus Mortierella elongata FMR23-6 harbors an endobacterium inside its mycelium. Attempts to isolate the endobacterium from the fungus were not yet successful, but a highly purified bacterial fraction was prepared. Here, we report the draft genome sequence of the endobacterium., American Society for Microbiology
    Genome Announcements, 2014, [Reviewed]
  • Alsobacter metallidurans gen. nov., sp. nov., a thallium-tolerant soil bacterium in the order Rhizobiales
    Zhihua Bao; Yoshinori Sato; Reiko Fujimura and Hiroyuki Ohta, A thallium-tolerant, aerobic bacterium, designated SK200a-9T, isolated from a garden soil sample was characterized by polyphasic approaches. From the results, the new genus and species, Alsobacter metallidurans gen. nov., sp. nov. is proposed for strain SK200a-9T in the order Rhizobiales. The type strain is SK200a-9T (=NBRC 107718T =CGMCC 1.12214T)., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 2014, [Reviewed]
  • Characterization of Early Microbial Communities on Volcanic Deposits along a Vegetation Gradient in the Island of Miyake, Japan
    Yong Guo; Reiko Fujimura; Yoshinori Sato; Wataru Suda; Seok-Won Kim; Kenshiro Oshima; Masahira Hattori; Takashi Kamijyo; Kazuhiko Narisawa; and Hiroyuki Ohta, Corresponding, The 2000 eruption of Mount Oyama in the Island of Miyake (Miyake-jima) created a unique opportunity to study the early ecosystem development on newly exposed terrestrial substrates. In this study, bacterial and fungal communities on 9- and 11-year-old volcanic deposits at poorly to fully vegetation-recovered sites in Miyake-jima, Japan, were characterized by conventional culture-based methods and pyrosequencing of 16S rRNA and 18S rRNA genes., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, 2014, [Reviewed]
  • Temporal and spatial of fine sediment of Tonegawa River estuary at flushed and static periods and their bacterial community structures
    坂上伸生; 郭 永; 箕浦靖久; 太田寛行; 佐藤嘉則; 渡邊眞紀子; 石川忠晴, Properties of estuary sediment change dramatically by repetition of flushing events under management of the river flow. The dynamics of fine sediments in the Tonegawa river estuary were studied from analyses of physicochemical properties and bacterial community structure of sediments throughout flushing, static and transitional periods. In flushed period, coarse sand occupied the estuary sediment in place of fine sediment flushing out to the sea with flood. In static period, fine sediment dominated in almost all points and sedimentation of labile organic matter (LOM) and amorphous iron (Feo) gradually spread from river mouth towards upstream in connection with the intrusion of saline wedge and the estuary circulation. The following explanation for chemical dynamics of estuary environment in the Tonegawa river was lead from the evidence obtained by bacterial community structure. The rapid decomposition of LOM by sulfate and/or sulfide reducing bacteria generated anaerobic condition with produce of hydrogen sulfide and release of reductive Fe(II), which reacted to dominate precipitation of iron sulfide. The chemical properties of estuary sediment were strongly influenced by the river flow and the dynamics of saline wedge., SOCIETY OF ENVIRONMENTAL SCIENCE, JAPAN
    環境科学会誌, Mar. 2013, [Reviewed]
  • Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
    Yoshinori Sato; Hiroyuki Ohta; Takao Yamagishi; Yong Guo; Tomoyasu Nishizawa; M. Habibur Rahman; Hisao Kuroda; Task Kato; Masanori Saito; Ikuo Yoshinaga; Kazuyuki Inubushi; Yuichi Suwa, An anammox assay involving a N-15 tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N-2 production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca. 'B. anammoxidans', and Ca. 'Kuenenia stuttgartiensis'. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    MICROBES AND ENVIRONMENTS, Sep. 2012, [Reviewed]
  • The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage
    Rida O. Khastini; Hiroyuki Ohta; Kazuhiko Narisawa, The soil-inhabiting fungal pathogen Fusarium oxysporum has been an increasing threat to Chinese cabbage (Brassica campestris L.). A dark septate endophytic fungus, Veronaeopsis simplex Y34, isolated from Yaku Island, Japan, was evaluated in vitro for the ability to suppress Fusarium disease. Seedlings grown in the presence of the endophyte showed a 71% reduction in Fusarium wilt disease and still had good growth. The disease control was achieved through a synergetic effect involving a mechanical resistance created by a dense network of V. simplex Y34 hyphae, which colonized the host root, and siderophore production acting indirectly to induce a resistance mechanism in the plant. Changes in the relative abundance of the fungal communities in the soil as determined by fluorescently labelled T-RFs (terminal restriction fragments), appeared 3 weeks after application of the fungus. Results showed the dominance of V. simplex Y34, which became established in the rhizosphere and out-competed F. oxysporum., MICROBIOLOGICAL SOCIETY KOREA
    JOURNAL OF MICROBIOLOGY, Aug. 2012, [Reviewed]
  • Complete genome sequence of Leptospirillum ferrooxidans strain C2-3 isolated from fresh volcanic ash deposit on the island of Miyake, Japan
    Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Kenshiro Oshima; Seok-Won Kim; Masahira Hattori; Takashi Kamijo and Hiroyuki Ohta, Corresponding, A diazotrophic acidophilic iron-oxidizing bacterium, Leptospirillum ferrooxidans, known to be difficult to cultivate, was isolated from a fresh volcanic ash deposit on the island of Miyake, Japan. Here, we report the complete genome sequence of a cultured strain C2-3., AMER SOC MICROBIOLOGY
    Journal of Bacteriology, Aug. 2012, [Reviewed]
  • Analysis of Early Bacterial Communities on Volcanic Deposits on the Island of Miyake (Miyake-jima), Japan: a 6-year Study at a Fixed Site
    Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Kenji Nanba; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta, Corresponding, Microbial colonization on new terrestrial substrates represents the initiation of new soil ecosystem formation. In this study, we analyzed early bacterial communities growing on volcanic ash deposits derived from the 2000 Mount Oyama eruption on the island of Miyake (Miyake-jima), Japan. A site was established in an unvegetated area near the summit and investigated over a 6-year period from 2003 to 2009. Collected samples were acidic (pH 3.0-3.6), did not utilize any organic substrates in ECO microplate assays (Biolog), and harbored around 10(6) cells (g dry weight)(-1) of autotrophic Fe(II) oxidizers by most-probable-number (MPN) counts. Acidithiobacillus ferrooxidans, Acidithiobacillus ferrivorans, and the Leptospirillum groups I, II and III were found to be abundant in the deposits by clone library analysis of bacterial 16S rRNA genes. The numerical dominance of Acidithiobacillus ferrooxidans was also supported by analysis of the gene coding for the large subunit of the form I ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). Comparing the 16S rRNA gene clone libraries from samples differing in age, shifts in Fe(II)-oxidizing populations seemed to occur with deposit aging. The detection of known 16S rRNA gene sequences from Fe(III)-reducing acidophiles promoted us to propose the acidity-driven iron cycle for the early microbial ecosystem on the deposit., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    MICROBES AND ENVIRONMENTS, Mar. 2012, [Reviewed]
  • Study on the No-tillage, Weed-cover and Low-input Farming System for Organic Vegetable Production : A Case Study in Ibaraki, Japan               
    小松崎将一; 山下幸祐; 竹崎善政; 嶺田拓也; 金子信博; 中島紀一; 太田寛行
    有機農業研究, 2012, [Reviewed]
  • Fuel ethanol production from sweet sorghum using repeated-batch fermentation
    Shigeru Chohnan; Megumi Nakane; M. Habibur Rahman; Youji Nitta; Takanori Yoshiura; Hiroyuki Ohta; Yasurou Kurusu, Ethanol was efficiently produced from three varieties of sweet sorghum using repeated-batch fermentation without pasteurization or acidification. Saccharomyces cerevisiae cells could be recycled in 16 cycles of the fermentation process with good ethanol yields. This technique would make it possible to use a broader range of sweet sorghum varieties for ethanol production. (C) 2010, The Society for Biotechnology, Japan. All rights reserved., SOC BIOSCIENCE BIOENGINEERING JAPAN
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Apr. 2011, [Reviewed]
  • Limnobacter litoralis sp. nov., a thiosulfate-oxidizing heterotrophic bacterium isolated from volcanic deposits on Miyakejima, Japan
    Lu; H; Y. Sato; R. Fujimura; T. Nishizawa; T. Kamijo; H. Ohta, Corresponding, A Gram-negative, aerobic, heterotrophic bacterium able to use thiosulfate (optimum concentration, 10 mM) as an additional energy source, designated KP1-19T, was isolated from a 22-year-old volcanic deposit at a site lacking vegetation on the island of Miyake, Japan. In this study, its taxonomic position was determined by a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain KP1-19T is closely related to Limnobacter thiooxidans LMG 19593T, the single species in the genus Limnobacter within the class Betaproteobacteria. The level of 16S rRNA gene sequence identity between strains KP1-19T and LMG 19593T was 97.7%. The major cellular fatty acids in KP1-19T were found to be C16:1ω7c, C18:1ω7c, C16:0 and C10:0 3-OH, which are characteristic of the genus Limnobacter. The DNA-DNA relatedness of strains KP1-19T with LMG 19593T is 18%. Therefore, strain KP1-19T represents a novel species, for which the name Limnobacter litoralis sp. nov. is proposed; the type strain is strain KP1-19T (=LMG 24869T =NBRC 105857 T =CIP 109929T)., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 18 Feb. 2011, [Reviewed]
  • Distribution of inorganic components of fine sediment of Tone river estuary and their microbial diversity
    SAKAGAMI Nobuo; YAMAZAKI Ken-ichi; SATO Yoshinori; OHTA Hiroyuki; WATANABE Makiko; ISHIKAWA Tadaharu, 【背景と目的】 河川上中流部から供給される細粒土砂は,感潮域に至って底質の主要成分を構成する。しかしながら,堆積量に比較して通過量が多いことから,その動態を把握することは困難である。河川底質の化学的特性はシルト以下の細粒画分に帰する場合が多く,その動態は底質環境を特徴付ける。山崎ほか(2010)は利根川感潮域の底質を構成する有機物に着目し,出水により低下した炭素量は河口側および上流側の双方から回復して平水時に至ることを明らかにしている。本研究では出水時と平水時の細粒底質中の無機成分の縦断的分布と平水時の微生物多様性について調べた。
    【調査地域と研究方法】 底質調査は,大きな出水直後(最大流量:8,300m3/s)の2008年9月,移行期間である10月,2009年2月の平水時に利根川河口から2KP(KPは河口からの距離)~27KPまでの澪筋で縦断的に行った。採取はエクマンバージ採泥器を用い,層を成している場合は2層に別けて採取した。試料は恒温炉(60℃)で乾燥させ,125umの金属製篩を通過した画分についてエネルギー分散型蛍光X線元素分析装置(EDX-700HS, 島津製作所)によりXRF分析を行った。試料は直径2cm,深さ0.5mmのアルミリングに入れ,100KNで加圧成形して分析に供試した。
    2010年6月(概ね平水時に該当)に4KP,河口堰に隣接する上流部(およそ18.5KP),27KPにおける底質を採取し,細菌密度と細菌多様性について解析した。4KPおよび堰試料については1層目と2層目をそれぞれ分析に供試した。DNA抽出はISOIL for Beads Beating(NIPPON GENE)で行った。細菌量(copies/g湿重)は16S rRNA遺伝子の定量PCR測定から求め,細菌多様性はPCR増幅した16S rRNA遺伝子群を末端断片長多型(T-RFLP)分析に供して推定した。
    【結果と考察】 細粒底質のXRF分析の結果,40~60%がSiで,Fe,Alが10~30%程度検出された。また,Ti,Ca,K,S,Mgが数%程度,Mn,Zr,Cu,Zn,Rbが1%以下検出された。また海水の影響と考えられるBr,Clがそれぞれ最大0.1,3.6%程度検出される場合があった。それぞれの元素の強度値を用いて主成分分析(SPSS ver. 10.0.5J)を行ったところ,Fe・Mn酸化物(第1成分),アルミノケイ酸鉱物(第2成分),ハロゲン(第3成分)によって特徴付けられる成分が抽出された。図1にFe・Mn酸化物成分およびハロゲン成分の出水時および平水時の縦断分布を示す。出水によりFe・Mn酸化物は攪乱され,ハロゲンは数地点で2層目に残留しているものの,多くの地点で消失していた。Fe・Mn酸化物は陸域に由来し,平水時には上流側で高かった。また,主に海域に由来すると考えられるハロゲンが平水時に下流側で高くなる傾向が見られた。河口付近ではエスチュアリ循環により海由来有機物を多く含む細粒底質が遡上することが確認されている(清水ほか,2004)。本研究でも平水時にかけて,20KP付近まで海から遡上した塩水に由来する細粒分が支配的となっていくことが確認された。
    細菌量は,27KPの底質では下流側に比べて約100倍低いレベルであった。これはシルト質成分が少なく炭素量が小さいことに起因すると考えられたが,細菌量と全炭素量との間にはあまり明瞭な対応関係がみられなかった。T-RFLP分析でみた細菌多様性は4KP,18.5KP,27KPの地点間で差はみられなかったが,細菌群集構造については地点間で顕著な違いがみられた。なお,各地点の1層目と2層目には大きな違いがないことが明らかとなった。感潮域における河川流動と底質環境を反映した微生物分布特性が予想された。
    【参考文献】 清水ほか(2004)水工学論文集,48,769-774/山崎ほか(2010)社団法人環境科学会2010年会プログラム,p.97, The Association of Japanese Geographers
    Proceedings of the General Meeting of the Association of Japanese Geographers, 2011
  • Molecular characterization of fungal communities in non-tilled, cover-cropped upland rice field soils
    Nishizawa; T.; Zhaorigetu; M. Komatsuzaki; Y. Sato; N. Kaneko; H. Ohta, Last, This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth years were analyzed using T-RFLP (terminal restriction fragment length polymorphism) profiling and clone libraries with the internal transcribed spacer (ITS) region and domain 1 (D1) of the fungal large-subunit (fLSU) rRNA (D1(fLSU)) as the target DNA sequence. From the 2nd-year-sample, 372 cloned sequences of fungal ITS-D1(fLSU) were obtained and clustered into 80 non-redundant fungal OTUs (operational taxonomic units) in 4 fungal phyla. The T-RFLP profiling was performed with the 2nd- and 5th-year-samples and the major T-RFs (terminal restriction fragments) were identified using a theoretical fragment analysis of the ITS-D1(fLSU) clones. These molecular analyses showed that the fungal community was influenced more strongly by the cover-cropping than tillage practices. Moreover, the non-tilled, cover-cropped soil was characterized by a predominance of Cryptococcus sp. in the phylum Basidiomycota. We provided a genetic database of the fungal ITS-D1(fLSU)s in the differently managed soils of upland rice fields., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, 01 Sep. 2010, [Reviewed]
  • Isolation and molecular characterization of a multicellular cyanobacterium, Limnothrix/Pseudanabaena sp. strain ABRG5-3.
    Nishizawa, T; T. Hanami; E. Hirano; T. Miura; Y. Watanabe; A. Takanezawa; M. Komatsuzaki; H. Ohta; M. Shirai; M. Asayama, 日本農芸化学会
    Biosci. Biotechnol. Biochem., 2010, [Reviewed]
  • Detection of betaproteobacteria inside the mycelium of the fungus Mortierella elongata soil isolates
    Sato; Y.; K. Narisawa; K. Tsuruta; M. Umezu; T. Nishizawa; K. Tanaka; K. Yamaguchi; M. Komatsuzaki; and H. Ohta, Corresponding, Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N(2)O-producing activity and the presence of endobacteria., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, 2010, [Reviewed]
  • Nitrogenase activity (acetylene reduction) of an iron-oxidizing Leptospirillum strain cultured as a pioneer microbe from a recent volcanic deposit on Miyake-jima, Japan
    Sato; Y.; K. Hosokawa; R. Fujimura; T. Nishizawa; T. Kamijyo; H. Ohta, Corresponding, The genus Leptospirillum is known to dominate acid mine drainage and bioleaching systems. In this paper, we describe the isolation of iron-oxidizing bacteria closely related to Leptospirillum ferrooxidans from an acidic volcanic ash deposit on the island of Miyake (Miyake-jima), Japan. We further show the nitrogenase activity (acetylene reduction) for one (strain C2-1) of the isolates. The deposit harbored 1.2 x 10(8) total direct count (g dry weight)(-1) and 7.1 x 10(5) (most-probable-number, MPN) (g dry weight)(-1) of iron-oxidizer. A ferrous iron-limited, aerobic chemostat culture using Leptospirillum HH medium (pH 1.8) was performed to select and isolate the Leptospirillum group. Nine isolates were regarded as pure cultures based on uniform colony morphology on Fe(2+)-containing silica gel plates and absence of growth on 100-fold diluted nutrient broth plates. Six strains examined further shared 99.9-100% identity in 16S rRNA gene sequence with each other. Homology-based searches showed that all of the strains belonged to the Leptospirillum ferrooxidans clade. Strain C2-1 grown in ammonium sulfate-free Leptospirillum HH medium (pH 1.8) showed an initial rate of acetylene reduction of 58 mu mol h(-1) (g cell carbon)(-1)., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, 01 Dec. 2009, [Reviewed]
  • 森林土壌に形成される菌核の微生物担体としての有効性評価               
    野々山弥; 太田寛行; 成澤才彦; 渡邉眞紀子
    生物工学会誌, 24 Nov. 2009, [Reviewed]
  • Atmospheric CO and hydrogen uptake and CO oxidizer phylogeny for Miyake-jima, Japan volcanic deposits
    King; G. M.; C. F. Weber; K. Nanba; Y. Sato and H. Ohta, We have assayed rates of atmospheric CO and hydrogen uptake, maximum potential CO uptake and the major phylogenetic composition of CO-oxidizing bacterial communities for a variety of volcanic deposits on Miyake-jima, Japan. These deposits represented different ages and stages of plant succession, ranging from unvegetated scoria deposited in 1983 to forest soils on deposits > 800 yr old. Atmospheric CO and hydrogen uptake rates varied from -2.0 +/- 1.8 6.3 +/- 0.1 mg CO m (2) d(-1) and 0.0 +/- 0.4-2.0 +/- 0.2 mg H(2) m(-2) d(-1), respectively, and were similar to or greater than values reported for sites on Kilauea volcano, Hawaii, USA. At one of the forested sites, CO was emitted to the atmosphere, while two vegetated sites did not consume atmospheric hydrogen, an unusual observation, Although maximum potential CO uptake rates were also comparable to values for Kilauea, the relationship between these rates and organic carbon contents of scoria or soil indicated that CO oxidizers were relatively more abundant in Miyake-jima deposits. Phylogenetic analyses based on the large sub-unit gene for carbon monoxide dehydrogenase (coxL) indicated that many novel lineages were present on Miyake-jima, that CO-oxidizing Proteobacteria were prevalent in vegetated sites and that community structure appeared to vary more than composition among sites., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, Dec. 2008, [Reviewed]
  • Utilization of phenylpropanoids by Pseudomonas putida soil isolates and its probable taxonomic significance
    Kazuhiro Nonaka; Hiroyuki Ohta; Yoshinori Sato; Keiichi Hosakawa, Corresponding, Twenty seven soil bacterial isolates able to grow on cinnamic acid or ferulic acid as a sole carbon and energy source were examined for the ability to utilize other phenylpropanoids and phylogenetic interrelationships. A 16S rRNA gene sequence analysis revealed that 26 of the isolates were closely related to Pseudomonas putida biovar A and classified into 6 OTUs: cinnamic acid utilization-positive (OTUs, CN-1 to CN-3) and -negative groups (FR-1 to FR-3). P. putida NBRC 14164(T) and JCM 13061 belonged to FR-3. A probable reclassification of P. putida was discussed in relation to the utilization of phenylpropanoids., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, Dec. 2008, [Reviewed]
  • Relationships between Fungal Biomass and Nitrous Oxide Emission in Upland Rice Soils under No Tillage and Cover Cropping Systems
    Zhaorigetu; Masakazu Komatsuzaki; Yoshinori Sato and Hiroyuki Ohta, Corresponding, The relationships between soil microbial properties and nitrous oxide emission were examined in upland Soil under different tillage systems [no tillage (NT), rotary and plow tillage] and cover crop systems (fallow, cereal rye, and hairy vetch) in 2004 and 2005. Microbiological analyses included the determination of soil ergosterol as all indicator of fungal biomass, bacterial plate counting, and MPN estimations of ammonia oxidizers and denitrifiers. The combined practice of NT with rye-cover crop treatment increased fungal biomass but not bacterial populations in 0-10 cm deep soils. Such increase in fungal biomass was not found in 10-20 cm and 20-30 cm deep cover-cropped NT soil. The combined practice of NT with rye-cover cropping resulted in higher in situ N(2)O emission rates compared with rotary- and plow-till treatments. N(2)O flux was positively correlated with soil ergosterol content but not with denitrifier MPN and other soil chemical properties. These results suggested a significant contribution of fungi to N(2)O emission in cover-cropped NT soils., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, Sep. 2008, [Reviewed]
  • Archaeal Diversity of Upland Rice Field Soils Assessed by the Terminal Restriction Fragment Length Polymorphism Method Combined with Real Time Quantitative-PCR and a Clone Library Analysis
    Nishizawa; T.; M. Komatsuzaki; N. Kaneko; H. Ohta, The PCR amplification-based analysis of microbial diversity is subject to potential problems. In this study, to minimize the bias toward a 1: 1 ratio in multitemplate PCR, a real-time PCR assay was carried out using a quenching fluorescence dye primer and amplification efficiency was monitored. Then terminal-restriction fragment length polymorphism (T-RFLP) profiling was performed using the PCR product with minimized PCR bias. This method was applied to an analysis of the diversity of the archacal community in an upland rice field under different tillage systems and winter cover cropping. Terminal restriction fragments (T-RFs) of PCR-amplified archacal 16S rRNA genes were assigned to the gene sequences recovered from the same soil by using an archaeal 16S rRNA gene clone library. Our results indicated that soil archaeal members were not influenced but the relative abundance of archaeal species particularly those belonging to Crenarchaeota which changed between the tillage and non-tillage treatments., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, Sep. 2008, [Reviewed]
  • Characterization of Herbaspirillum- and Limnobacter-Related Strains Isolated from Young Volcanic Deposits in Miyake-jima Island, Japan
    H. LU; R. FUJIMURA; Y. SATO; K. NANBA; T. KAMIJO and H. OHTA, Corresponding, The role of microbes in the early development of ecosystems on new volcanic materials seems to be crucial to primary plant succession but is not well characterized. Here we analyzed the bacterial community colonizing 22-year-old volcanic deposits of the Miyake-jima Island (Japan) using culture-based and 16S rRNA gene clone library methods. The majority of 91 bacterial isolates were placed phylogenetically in two clusters (A and B) of the Betaproteobacteria. Cluster A (82% of isolates) was related to the genus Limnobacter and Cluster B (9%) was affiliated with the Herbaspirillum clade. The clone library analysis supported the predominance of Cluster B rather than Cluster A. Strain KP1-50 of Cluster B was able to grow on a mineral medium under an atmosphere of H-2, O-2, and CO2 (85: 5: 10), and characterized by its large-subunit gene of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL) and nitrogenase reductase gene (nifH). In contrast, strains of Cluster A did not grow chemolithoautotrophically with H-2, O-2, and CO2 but increased their cell biomass with the addition of thiosulfate to the succinate medium, suggesting the use of thiosulfate as an energy source. From phenotypic characterization, it was suggested that the Cluster A and B strains were novel species in the genus Limnobacter and Herbaspirillum, respectively., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, Mar. 2008, [Reviewed]
  • 三宅島2000年噴火堆積物の初成土壌形成と細菌生態系(2008年度大会一般講演要旨)
    藤村 玲子; 佐藤 嘉則; 長澤 俊樹; 細川 研; 西澤 智康; 難波 謙二; 加藤 拓; 片山 葉子; 上條 隆志; 東 照雄; 太田 寛行, 日本土壌微生物学会
    土と微生物, 2008
  • Isolation of octylphenol polyethoxylate-degrading soil bacteria: a long-term soil column study
    Anne D. Rulianti; Morifumi Hasegawa; Yoko Ikunaga; Yoshinori Sato and Hiroyuki Ohta, Corresponding, A soil column was percolated with Triton X-100 (octylphenol polyethoxylates; OPEOn) under aerobic conditions for 300 days and OPEOn-degrading bacteria were isolated and characterized phylogenetically. Populations of total culturable bacteria and OPEOn (1000 mg L-1)-resistant bacteria increased 10 times and 46 times, respectively, during the percolation. 16S rRNA gene sequencing of 128 OPEOn-resistant isolates revealed an increase in the population level of Alphaproteobacteria and decreases in levels of Betaproteobacteria and Actinobacteria with the exposure to the compound. Four OPEOn-degrading strains were isolated and characterized as being related to Bradyrhizobium liaoningense (3 strains) and Afipia sp. (1 strain)., JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Microbes and Environments, 01 Dec. 2007, [Reviewed]
  • Characterization of major and trace elements in sclerotium grains
    M. Watanabe; Y. Inoue; N. Sakagami; O. Bolormaa; K. Kawasaki; S. Hiraide; N. Fujitake; H. Ohta, Sclerotium grains in soil contain humus-metal complexes that are probably produced from fungal metabolites. The characterization of major elements in sclerotium grains collected from volcanic ash soils in Mt Myoko was examined by X-ray photoelectron spectroscopy, FT-IR spectrometry and CHN analysis, and the concentration of trace elements was determined by PIXE (particle induced X-ray emission spectrometer) analysis. The content of major elements, C, H, N, O and Al, was approximately 47.6, 3.32, 0.78, 30.2 and 1.4% by mass, respectively. Trace elements such as Ti, Cr, Mn, Cu, Zn, Br and Pb were detected in the grains at concentrations between 10 and 100 mu g g(-1). Functional carbon groups for the whole grain were characterized by the dominance of O-alkyl C associated with aromatic C. The comparison between the surface and subsurface (matrix) of the grain showed that the concentrations of O, C and N were relatively greater on the surface of sclerotium grains than in the matrix. The proportion of carbon having C-O, C=O, and O-C=O bonds, O and N showed a tendency to decrease from the surface towards the matrix. The proportion of C assigned as C-C and/or C-H bonds had a tendency to increase towards the matrix associated with Al., BLACKWELL PUBLISHING
    European Journal of Soil Science, 01 Jun. 2007, [Reviewed]
  • Soil management practices for sustainable agro-ecosystems
    M. Komatsuzaki, H. Ohta, Last, A doubling of the global food demand projected for the next 50 years poses a huge challenge for the sustainability of both food production and global and local environments. Today's agricultural technologies may be increasing productivity to meet world food demand, but they may also be threatening agricultural ecosystems. For the global environment, agricultural systems provide both sources and sinks of greenhouse gases (GHGs), which include carbon dioxide (CO(2)), methane (CH(4)), and nitrous oxide (N(2)O). This paper addresses the importance of soil organic carbon (SOC) for agro-ecosystems and GHG uptake and emission in agriculture, especially SOC changes associated with soil management. Soil management strategies have great potential to contribute to carbon sequestration, since the carbon sink capacity of the world's agricultural and degraded soil is 50-66% of the historic carbon loss of 42-72 Pg (1 Pg=10(15) g), although the actual carbon storage in cultivated soil may be smaller if climate changes lead to increasing mineralization. The importance of SOC in agricultural soil is, however, not controversial, as SOC helps to sustain soil fertility and conserve soil and water quality, and organic carbon compounds play a variety of roles in the nutrient, water, and biological cycles. No-tillage practices, cover crop management, and manure application are recommended to enhance SOC storage and to contribute to sustainable food production, which also improves soil quality. SOC sequestration could be increased at the expense of increasing the amount of non-CO(2) GHG emissions; however, soil testing, synchronized fertilization techniques, and optimum water control for flooding paddy fields, among other things, can reduce these emissions. Since increasing SOC may also be able to mitigate some local environmental problems, it will be necessary to have integrated soil management practices that are compatible with increasing SOM management and controlling soil residual nutrients. Cover crops would be a critical tool for sustainable soil management because they can scavenge soil residual nitrogen and their ecological functions can be utilized to establish an optimal nitrogen cycle. In addition to developing soil management strategies for sustainable agro-ecosystems, some political and social approaches will be needed, based on a common understanding that soil and agro-ecosystems are essential for a sustainable society., SPRINGER TOKYO
    Sustainability Science, 2007, [Reviewed]
  • 14C ages and δ13C of sclerotium grains found in forest soils
    Watanabe; M.; H. Sato; H. Matsuzaki; T. Kobayashi; N. Sakagami; Y. Maejima; H. Ohta; N. Fujitake; S. Hiradate, C-14 ages and delta C-13 were examined for sclerotium grains to elucidate the characteristics of these grains distributed in forest soils. The ages of the grains from surface A horizons and buried A horizons were ca 100-200 BP and ca 300-1,200 BP, respectively. In comparison with humic acid extracts, the C-14 ages were in the increasing order: humic acid fraction < humic acid Pg fraction < sclerotium grains. The delta C-13 values for sclerotium grains in surface A horizons and buried A horizons were approximately -31 parts per thousand to -28 parts per thousand, and these values were approximately 2-4 parts per thousand smaller than those of humic acids and soils. The C content of the grains had a tendency to decrease with increasing C-14 ages, while the C content of humic acid was constant with age. The C-14 ages of sclerotium grains indicate the individual age of grain formation, which are more likely to assign closer ages to the beginning of soil forming than the C-14 ages of humic acid. The low delta C-13 values for sclerotium grains have presumably originated from characteristically biological organics, which may be protected from attack in soils because of their structure., TAYLOR & FRANCIS LTD
    Soil Science and Plant Nutrition, 2007, [Reviewed]
  • Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., two novel hydrogen-oxidizing facultative chemolithotrophic bacteria isolated from Mt. Pinatubo volcanic mudflow deposits in the Philippines
    Sato; Y.; H. Nishihara; M. Yoshida; M. Watanabe; J. D. Rondal; R. N. Concepcion; H. Ohta, Corresponding, Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus Cupriavidus of the Betaproteobacteria; sequence similarity values with their nearest phylogenetic neighbour, Cupriavidus basilensis, were 97(.)1-98(.)3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA-DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related Cupriavidus species with validly published names. The isolates were aerobic, Gramnegative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65(.)2 to 65(.)9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, Cupriavidus pinatubonensis sp. nov. [nine strains, with 1245(T) (=CIP 108725(T)= PNCM 10346(T)) as the type strain] and Cupriavidus laharis sp. nov. [one strain, the type strain 1263a(T) (=CIP 108726(T)= PNCM 10347(T))]. It is also suggested that Ralstonia sp. LMG 1197 (=JMP 134) should be included in the species C. pinatubonensis., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 2006, [Reviewed]
  • Vertical distribution of chloroform-extractable green fraction (CEGF)in several Andosol and Cambisol profiles
    Kobayashi; T.; M. Watanabe; H. Ohta; and N. Fujitake, The vertical distribution of the content of the chloroform-extractable green fraction (CEGF) in the soil profiles of four Japanese Andosols, one Japanese Cambisol and one Nepalese Cambisol was examined using a colorimetric method with an ultraviolet and visible spectrophotometer. In addition, the relationship between the CEGF content and the soil chemical properties was examined by statistical analysis. CEGF was detected in all the soil samples, and the content ranged from 0.02 to 1.16 g kg(-1). The CEGF content in the surface A horizon of the Andosol tended to be higher than in the Cambisol. The vertical distribution of the CEGF content in three soil profiles (two Andosol profiles and one Nepalese Cambisol) revealed maximum values in the subsurface horizon. In the Japanese Cambisol, the content of the CEGF was highest in the surface horizon. In the remaining two Andosol profiles containing a buried humus horizon, the content of the CEGF was highest in the buried humus horizon and the distribution was considerably different from that of organic carbon. Statistical analysis showed that the content of the CEGF displayed a significant positive correlation (P < 0.001, n = 36) with the contents of dithionite-citrate-bicarbonate-extractable Al (Ald), and with oxalate-oxalic acid-extractable Fe and Al (Feo and Alo, respectively). Furthermore, the content of the CEGF showed a significant positive correlation with the content of Alo (r = 0.944, P < 0.01, n = 6) in the soil with the highest content of CEGF in the profile. It was, therefore, suggested that CEGF is actively produced in humus-rich soils like Andosols, and that Ald, Feo and, in particular, Alo were associated with the vertical distribution of CEGF in the soil profiles., BLACKWELL PUBLISHING
    Soil Science and Plant Nutrition, 2006, [Reviewed]
  • Sphingomonas soli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in the alpha-4 subclass of the Proteobacteria
    D.-C. Yang; W.-T. Im; M. K. Kim; H. Ohta; S.-T. Lee, Strain T5-04(T), a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain T5-04T belongs to the U-4 subgroup of the Proteobacteria, and the highest degrees of sequence similarity determined were to Sphingomonas asaccharolytica IFO 10564(T) (97.5%), Sphingomonas koreensis JSS26(T) (97(.)1%), Sphingomonas mali IFO 15500(T) (96(.)7%) and Sphingomonas pruni IFO 15498(T) (96(.)6%). Chemotaxonomic data revealed that strain T5-04(T) possesses ubiquinone Q-10 predominantly, C-18:1 as the predominant fatty acid and sphingoglycolipids, all of which corroborate its assignment to the genus Sphingomonas. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain T5-04(T) represents a distinct species. Based on polyphasic; evidence, T5-04(T) (= KCTC 12210(T) NBRC 10080(T)= IAM 15213(T)) should be classified as the type strain of a novel Sphingomonas species, for which the name Sphingomonas soli sp. nov. is proposed., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 2006
  • Genetic transformation system for members of the genera, Sphingomonas, Sphingobium, Novosphingobium, and Sphingopyxis
    Miyuki Saito; Yoko Ikunaga; Hiroyuki Ohta and Yasurou Kurusu, This paper describes a plasmid transformation system that permits genetic manipulation of the genus Sphingomonas. A cryptic indigenous plasmid, pAMI-1, of 10 kb from Sphingobium amiense was isolated and characterized. Nucleotide sequencing revealed that pAMI-1 contains five open reading frames, which are predicted to encode proteins associated with integration, recombination, conjugation and replication. Escherichia coli-S. amiense shuttle vectors were successfully introduced into Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis strains by electroporation. The copy number of the shuttle vector was estimated to be 1-2 per chromosome in the Sphingobium yanoikuyae cell. © 2006, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2006, [Reviewed]
  • Isolation and characterization of thallium-tolerant bacteria from heavy metal-polluted river sediment and non-polluted soils
    Zhihua Bao; Yoshinori Sato; Masatsugu Kubota and Hiroyuki Ohta, Thallium (Tl) is a heavy metal found in trace amounts in the earth's crust and has been studied to a much lesser degree than other heavy metals. Since the discovery of high-temperature superconducting components in the Tl-Ca-Ba-Cu-O system, Tl has attracted greater attention as a potential major pollution source of the future. In this study, the response of soil culturable bacterial communities to the addition of Tl was examined with fresh samples of non-polluted garden and arable soils. A preserved air-dried sample from heavy metal-polluted river sediment was also used as a reference. Soil suspension experiments were performed to homogenize the soils to evenly distribute both the microbial populations and Tl and reduce spatial variability. From soil suspensions loaded with Tl at 0.24 to 0.98 mM, bacteria tolerant to 0.49 mM Tl (100 mg Tl l-1) were isolated and characterized phylogenetically. The background level of culturable Tl-tolerant bacterial populations was 0.0003-0.013% for the non-polluted soils. The Tl-tolerant fraction increased rapidly in response to Tl loading and dominated the bacterial community, which might be attributed mainly to an immediate effect due to the death of Tl-sensitive microorganisms and leaking of nutrients from cell lysis of sensitive species supporting the growth of primarily Tl-tolerant heterotrophs. Such Tl-tolerant culturable bacteria were isolated and classified into 9 groups of Alphaproteobacteria, those from the arable soil were identified as Pseudomonas spp., and those from the contaminated river sediment were identified as Bacillus niacini. © 2006, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2006, [Reviewed]
  • Leukotoxin production by Actinobacillus actinomycetemcomitans
    Inoue; T.; K. Fukui; and H. Ohta, Actinobacillus actinomycetemcomitans is a gram-negative pathogen that causes human severe periodontitis. The most intensively studied virulence factor of this bacterium is a pore-forming cytotoxin termed leukotoxin. Production of leukotoxin varies considerably among A. actinomycetemcomitans strains. Interstrain variations in leukotoxin production are thought to derive from differences in structure of the leukotoxin promoter region. In addition, several studies showed that culture conditions are also important factors influencing leukotoxin production levels. Export of A. actinomycetemcomitans leukotoxin across the cell envelope is likely mediated by a type I secretion system. Furthermore, it has also been suggested that outer membrane vesicles are involved in extracellular localization of the leukotoxin. This review summarizes current knowledge on the genetic and environmental factors affecting leukotoxin production and the export pathway for extracellular localization of leukotoxin., TAYLOR & FRANCIS INC
    Toxin Reviews, 2006, [Reviewed]
  • Method for estimation the content of the chloroform-extractable green fraction (CEGF) in HCl-DMSO extract of soils               
    Kobayashi; T.; D. Asakawa; Y. Yanagi; T. Suzuki; M. Watanabe; H. Ohta; N. Fujitake
    Soil Science and Plant Nutrition, Nov. 2005, [Reviewed]
  • Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria
    Kim; M.K.; Im; W.T.; Ohta; H.; Lee; M.; Lee; S.T., Strain Kw07(T). a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery waste-water. 16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the α-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033(T) (97.8%). Chemotaxonomic data revealed that strain Kw07(T) possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C (18:1) ω 7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07(T) represents a distinct species. Based on these data, Kw07(T) (= KCTC 12209(T) = NBRC 100800(T)) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed., MICROBIOLOGY SOC KOREA
    The Journal of Microbiology, 2005, [Reviewed]
  • Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations
    Hiroyuki Ohta; Reiko Hattori; Yuuji Ushiba; Hisayuki Mitsui; Masao Ito; Hiroshi Watanabe; Akira Tonosaki and Tsutomu Hattori, The taxonomic position of a halo- and organo-sensitive, oligotrophic soil bacterium, strain S213(T), was investigated. Cells were Gram-negative, non-motile, strictly aerobic, yellow-pigmented rods of short to medium length on diluted nutrient broth. When 0(.)1 -0(.)4 % (w/v) NaCl was added to diluted media composed of peptone and meat extract, growth was inhibited with increasing NaCl concentration and the cells became long aberrant forms. When 6 mM CaCl2 was added, the cells grew quite normally and aberrant cells were no longer found at 0(.)1-0(.)5 % (w/v) NaCl. Chemotaxonomically, strain S213(T) contains chemical markers that indicate its assignment to the Sphingomonadaceae: the presence of ubiquinone Q-10 as the predominant respiratory quinone, C-18:1 and C-16:0 as major fatty acids, C-14:0 2-OH as the major 2-hydroxy fatty acid and sphingoglycolipids. 16S rRNA gene sequence analysis indicated that strain S213(T) belongs to the genus Sphingomonas, exhibiting high sequence similarity to the 1 6S rRNA gene sequences of Sphingomonas mali IFO 15500(T) (98(.)3 %), Sphingomonas pruni IFO 15498(T) (98(.)0%), Sphingomonas asaccharolytica IFO 15499(T) (97.9%) and Sphingomonas echinoides DSM 1805(T) (97-8%). The results of DNA-DNA hybridization experiments and its phenotypic characteristics clearly distinguished the strain from its nearest neighbours and demonstrate that strain S213(T) represents a novel Sphingomonas species, for which the name Sphingomonas oligophenolica sp. nov. is proposed. The type strain is S213(T) (=jCM 12082(T)=Clp 107926(T))., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 2004
  • Ecophysiology of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans with special emphasis on leukotoxin production
    Yuuji Ushiba and Hiroyuki Ohta, Actinobacillus actinomycetemcomitans, a Gram-negative periodontopathic bacterium, produces a leukotoxin belonging to the RTX family. The leukotoxin determinant consists of four contiguous genes designated ltxC, ltxA, ltxB and ltxD. Although the ltx operon appears to be present in all A. actinomycetemcomitans strains, the level of toxin expression varies considerably between the strains. Several molecular genetic studies have indicated that the difference in leukotoxin production among strains was dependent on the structure of the leukotoxin promoter region. In addition, chemostat culture studies revealed that the production of leukotoxin was influenced greatly by various environmental factors, e.g. growth rate, oxygen, bicarbonate, external sugar level, and pH. In this paper, the state of knowledge of leukotoxin expression in A. actinomycetemcomitans is reviewed. © 2004, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2004
  • 非アロフェン黒ぼく土における菌核粒子の分布と土壌化学性との関係
    坂上伸生; 渡邊眞紀子; 太田寛行; 藤嶽暢英, Sclerotium grain is the resting body of ectomycorrhizal fungi found in forest soils. A melanic-spherical shape in approximately 1-2mm diameter characterizes the external feature of the grain, and a hollow structure with honeycomb transverse wall appears inside the grain. In our previous studies, we reported a high aluminum concentration inside sclerotium grains and suggested the close relationship between the status of active aluminum and the distribution of these grains in Andosols (Watanabe et al., 2001; 2002). Here we examined the chemical properties focused on active aluminum and carbon in several nonallophanic Andosols under forest vegetation for further discussions on the regulating factor of the distribution of the sclerotium grains. In each studied soil profile, the mean weight of sclerotium grain (mg grain^<-1>) had a tendency to increase with the content of exchangeable aluminum, content of total organic carbon and nitrogen, carbon content of humic acid extracted from soils. The ratio of Alp to total organic C(Al_p/T-C) showed a strong negative correlation between the mean weight sclerotium grains regardless of profiles. The bonding ratio of carbon and aluminum in soils was assumed to be one of the factor influencing the development of sclerotium grains., Japanese Society of Pedology
    ペドロジスト, 2004
  • オカボ栽培でのカバークロップ利用と耕うんシステムが耕地の土壌炭素と生物相に及ぼす影響
    辜松; 小松崎将一; 太田寛行; 本林隆; 森泉昭治; 山口昌良
    農作業研究, 2004
  • Occurrence of hydrogen-oxidizing Ralstonia species as primary microorganisms in the Mt.Pinatubo volcanic mudflow deposits
    Yoshinori Sato; Hirofumi Nishihara; Masao Yoshida; Makiko Watanabe; Jose D. Rondal and Hiroyuki Ohta, Ralstonia eutropha is an aerobic, hydrogen-oxidizing, facultative chemolithotrophic bacterium. Recently, R. eutropha-related bacteria have been found to predominate in the cultural bacterial communities of fresh mudflow deposits from Mt. Pinatubo, the Philippines. In the present study, the R. eutropha-related strains were examined for their chemolithotrophic growth in the presence of H-2, O-2, and CO2 (85:5:10) and for their phylogenetic relationship with R. eutropha. Eleven of the 12 strains tested grew in the presence of H-2, O-2, and CO2, although the growth was not as rapid as that of the well-characterized H-2-oxidizing strain, R. eutropha H16. To examine the hydrogenase activity, membrane and soluble fractions were prepared from R. eutropha-related strain 1245 and its activity was assayed with methylene blue and NAD as electron acceptors. A significant hydrogenase activity was detected in both membrane and soluble fractions of the cell-free extract. The 16S rDNA analysis of the 12 strains of R. eutropha-related bacteria revealed that they could be classified into two clusters, both of which were clearly separated from the cluster of R. eutropha sensu stricto. The probable ecological niche of H2-oxidizing Ralstonia species in the volcanic mudflow deposits was examined., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2004
  • Soil chemical properties and distribution of sclerotium grains in forest soils, Harz Mts., Germany
    Makiko Watanabe; Shunpei Ohishi; Angelika Pott; Ulrike Hardenbicker; Kumiko Aoki; Nobuo Sakagami; Hiroyuki Ohta and Nobuhide Fujitake, Relationships between the distribution of fungal sclerotium grains and soil chemical properties were studied in forest soils of Podsole, Braunfahlerde, and Braunerde-Podsoles in Harz Mts., Germany. Development of sclerotium grains was dominant in surface horizons (Ah, E horizons) within a 10-cm depth and weight density of grains ranged from 0.01 to 4.99 g kg(-1) soil. The SEM-EDX analysis proved that the weight percentage (excluding C and N) of Al2O3 was 39.8-63.9% inside the grains. The content of elgosterol, a biomarker of viable fungal biomass, showed good correlations with weight density of sclerotium grains in grain-detected soils. The sclerotium grains were likely to be formed in soils with high ratios (>0.6) of organic bonding Al (Al-p) to amorphous Al (Al-o), and with high contents of exchangeable Al (Al3+) (>0.54 g kg(-1)). The content and state of active Al were believed to be responsible for the development of sclerotium because sclerotium grains were not detected in acid soils which had lower levels of free colloidal Al (Al-d, Al-o, Al-p). We believed that the intensive clay destruction associated with past lessivage process induced the absence of free colloid Al in such forest soils., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2004
  • Degradation profiles of branched nonylphenol isomers by Sphingobium amiense and Sphingomonas cloacae
    Yoko Ikunaga; Shu-ichi Miyagawa; Morifumi Hasegawa; Yasuhiro Kasahara; Osamu Kodama and Hiroyuki Ohta, Biodegradation activity of branched nonylphenols (BNPs) by Sphingobium amiense strain YTT and Sphingomonas cloacae strain S-3(T) was examined. High-resolution GC-MS was used to separate five isomer groups from the commercial BNP mixture. In the time course experiments of BNP degradation, the isomers with alpha-dimethyl and those with alpha-methyl, alpha-ethyl configurations were rapidly degraded by Sphingobium amiense strain YTT, while those with highly branched chains were broken down less efficiently. In contrast, Sphingomonas cloacae strain S-3(T) degraded all the isomers of the BNP groups at similar rates. The utilization and degradation of 4-n-alkylphenols (C3 to C9) and 8 phenolic acids were also tested with the strains. The results suggested that Sphingomonas cloacae strain S-3(T) displays a catabolic activity possesses the catabolism specific of branched alkylphenols, while Sphingobium amiense strain YTT is a versatile organism capable of utilizing a wide range of phenolic compounds including phenolic acids, and of degrading 4-n-alkylphenols and a limited range of BNPs., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2004
  • Aluminum oxyhydroxide polymorphs and some micromorphological characteristics in sclerotium grains
    Makiko Watanabe; Akira Genseki; Nobuo Sakagami; Yudsuru Inoue; Hiroyuki Ohta and Nobuhide Fujitake, The presence of aluminum oxyhydroxide and its micromorphological characteristics in sclerotium. grains were examined by SEM, TEM, and EDX analyses. A spherical fraction with a diameter of 100 nm, which contained C and Al as the predominant elements, was recognized as the unit particle of the matrix of sclerotium grains. The Al polymorphs and the Si spherical structures found in the ignited grains were assumed to correspond to boehmite and opaline silica particles, respectively. SEM analysis revealed the presence of a needle ball structure inside the hollow part of sclerotium grains. The relationship between the inner structure of sclerotium and biochemical processes of the host fungi was discussed., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2004
  • バングラデシュ、マルア村土壌のヒ素及び重金属元素含有量および米中のヒ素含有量
    Jesmin M. Sabina; 太田寛行; 佐合隆一; 平田 熙; 久保田正亜, 日本環境学会
    人間と環境, 2004, [Reviewed]
  • Quinone profiling of bacterial populations developed in the surface layen of volcanic mud flow deposits from Mt.Pinatubo (the Philippines)
    第1著者、他11名, Lead, The diversity of bacterial populations developed in the surface layer (0-0.25 m depth) of volcanic mudflow deposits from the Mt. Pinatubo volcano (the Philippines) was investigated using quinone profiling. Samples were collected from sites (named N and S1) that had been hit repeatedly by mudflows during successive rainy seasons after the violent eruption of 1991 and also from sites (F1 and F3) covered by mudflow in 1991 but with no deposition in following years. The total microscopic count ranged from 10(8) g(-1) (N and S1 sites) to 3.9 x 10(9) g(-1)(site F3). In the N sample only three quinone species were detected, while the quinone profiles of samples from sites S3 and F3 showed higher diversity. Tetrahydrogenated menaquinone with eight isoprenoid units [MK-8 (H-4)] was the predominant quinone species in the sample from site N, while MK-8, MK-8 (H-2), MK-8 (H-4), MK-9 (H-4) and MK-9 (H-8) were found as major quinones in the sample from site F3. Because these MK species are known to be the major respiratory quinones of the Actinobacteria, this bacterial group is expected to predominate in the land with primary vegetation recovery following the impact of the volcanic mudflow. (C) 2003 Elsevier Science Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
    Soil Biology and Biochemistry, 2003, [Reviewed]
  • Characterization of Sphingomonas species found as predominant members in the culturable bactevial community of a green pigment-containing sderotium grain from Mt.Myoko (Japan) volcanic ash soil
    Ohta; H.; M. Yagi; J. Suzuki; N. Fujitake; M. Watanabe, Lead, A small spherical black fungal sclerotium grain from podzolic soils, which was tentatively identified as the resting body of Cenococcum graniforme, was assumed as the source of green polynuclear quinone pigments in P type humic acid (K. Kumada and H.M. Hurst, Nature 214: 631-633, 1967). To examine the presence of bacteria inside sclerotium grains collected from an Andosol profile in Mt. Myoko, central Japan, the grains were repeatedly washed, ultrasonicated and then cultured on diluted nutrient broth. The sum of recovered bacteria as colony-forming units from the wash and ultrasonicate fractions was 1.46×106 (g fresh weight)-1: 88% of the count in the wash fractions (assumably resulting from grain surface and attached soil) and 12% in the ultrasonicate (inside grain). Thirty-one bacterial strains were isolated from the ultrasonicate fraction and their 16S rDNA partial sequences were determined. The predominant group was the Alphaproteobacteria (71%), chiefly the Sphingomonas group (52%). Representative isolates of the Sphingomonas group were examined for their ability to grow on naphthalenesulfonic acids as a model compound of polycyclic aromatic hydrocarbons (PAHs) and also on several phenolic acids. None of the isolates tested utilized the model PAH but many of them used p-hydroxy benzoic, vanillic, p-coumaric and ferulic acids for growth. Based on these results, the relationship between the predominance of Sphingomonas and the chemical character of the sclerotium grain was discussed. © 2003, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2003, [Reviewed]
  • Soil formation of new lahar materials derived from Mt.Pinatubo. I. Physical and chemical properties of new lahar materials and soils formed by old lahar materials
    Sasaki; R.; M. Yoshida; M. Miyahira; H. Ohta; M. Watanabe; S. Suzuki, The early stage of weathering/soil formation processes from new volcanic mudflow (new lahar materials) brought from Mt. Pinatubo was examined. For this purpose, comparative studies on the physical/chemical characteristics of the new lahar materials and soils formed by the deposition of old lahar materials were conducted. Original soils contained less coarse sand, more silt, and clay than the new lahar materials. Increase in the clay content of the original soils reflected the progression of weathering. The occurrence of a higher leaching process of Fe, Ca, and Mg and of a moderate accumulation of organic matter in the original soils was suggested, based on the differences in the soil elemental composition and chemical properties. Values of base saturation and phosphate absorption coefficient (PAC) of the new lahar materials were 388% and 44, while those of all the original soils ranged 15-160% and 55-894, respectively. With the progression of weathering, the PAC value tended to increase gradually while the base saturation value decreased abruptly under the environmental conditions prevailing in the Philippines., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2003, [Reviewed]
  • The estimation of lahar surface moisture conditions for regetation recovery in Mt.Pinatubo lahar a Hected area by remote sensing
    M. Yoshida; H. Takahashi; H. Ohta; M. Watanabe; A. Inanaga and S. Suzuki, システム農学会
    Japanese Journal of Agricultural System Society, 2003, [Reviewed]
  • Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment
    Yuuji Ushiba; Yoshiharu Takahara and Hiroyuki Ohta, Corresponding, A nonylphenol-degrading bacterial strain (YTT) was isolated previously from a river sediment sample obtained in Ami-machi, lbaraki, Japan, and identified as a Sphingomonas species. In this study, the taxonomic relationship between strain YTT, a recently described nonylphenol-de grading strain, Sphingomonas cloacae, and Sphingobium yanoikuyae, which is phylogenetically related, was examined. Their phenotypic characteristics were compared and levels of DNA-DNA relatedness between these strains were determined. Based on the results of physiological and biochemical tests and DNA-DNA hybridization, it is proposed that strain YTT (= \AM 15006(T) = JCM 11777(T) = CIP 107839(T)) represents a novel species of the genus Sphingobium, Sphingobium amiense sp. nov., SOC GENERAL MICROBIOLOGY
    International Journal of Systematic and Evolutionary Microbiology, 2003, [Reviewed]
  • 森林土壌中の菌核様粒子から分離された細菌の特徴(2002年度大会一般講演要旨)
    鈴木 順子; 佐藤 嘉則; 藤嶽 暢英; 渡邊 眞紀子; 太田 寛行, 日本土壌微生物学会
    土と微生物, 2002
  • Molecular genotyping of isolates of actinobacillus actinomycetem comitans by pulsed-field gel electrophoresis after Separate digestion with Sse8387 I and Xho I
    M Matsuda; K Takayama; S Haga; S Kagawa; H Ohta; K Fukui; JE Moore, Genomic DNA from 18 Japanese clinical isolates of Actinobacillus actinomycetemcomitans was obtained from six periodontitis patients and analyzed using pulsed-field gel electrophoresis (PFGE) after separate digestion with Sse8387I and with XhoI, Three isolates from an identical patient were found to share an identical PFGE profile, and isolates from distinct patients were found to have PFGE profiles distinctly different from each other. Consequently, the 18 Japanese clinical isolates were discriminated into six distinct genotypes by means of PFGE,
    The genomic DNA from the other six reference strains (ATCC33384, ATCC43717, ATCC 43718, JCM2434, JCM2435 and Nig-1) was discriminated into six genotypes by the same PFGE methodology, and these six genotypes were found to be distinctly different from the six genotypes of the 18 Japanese clinical isolates described above,
    Serotyping demonstrated three PFGE genotypes in the serotype a strains, four the serotype b strains and three the serotype c strains.
    The present results clearly suggest that the PFGE procedure after separate digestion with Sse83871 and with XhoI has an excellent discriminatory power amongst strains and has a good genotypability for A. actinomycetemcomitans., WILEY-V C H VERLAG GMBH
    Journal of Basic Microbiology, 2002, [Reviewed]
  • Distribution and development of sclerotium grains as influenced by aluminum status in volcanic ash soils
    M Watanabe; T Kado; H Ohta; N Fujitake, The distribution of sclerotium grains, which are considered to be the resting bodies formed by fungi species, was studied in 5 Andosol profiles in central Japan. Sclerotium grains were detected in the surface A and buried A horizons of nonallophanic (Alp / Alo > 0.5) soils but not in allophanic (Alp / Alo < 0.5) soils. The maximum density of the sclerotium grains in each profile was approximately 2.5 g kg(-1). A highly positive relationship was observed between the mean weight of the sclerotium grains and the content of exchangeable aluminum (Al3+) in soils. Aluminum concentration inside of the sclerotium grains was confirmed by energy dispersive X-ray spectrometer (EDS) analysis. The results obtained suggest that the status of active Al3+ in soils is an important factor for sclerotium. development., TAYLOR & FRANCIS LTD
    Soil Science and Plant Nutrition, 2002, [Reviewed]
  • 日鉱金属佐賀関製錬所周辺土壌のアンチモン等金属元素による汚染               
    根目澤卓男; 太田寛行; 久保田正亜
    人間と環境, 2001, [Reviewed]
  • Aluminum concentrations in sclerotia from a buried humic horizon of volcanic ash soils in Mt. Myoko, Central Japan
    M Watanabe; N Fujitake; H Ohta; T Yokoyama, The morphology and chemical composition of fungal sclerotium-like grains collected from the buried humic horizon of volcanic ash soil in Mt, Myoko were examined using a scanning electron microscope (SEM): energy dispersion Xray micro-analyzer (EDX), and electron probe micro-analyzer (EPMA). A high C content, low level of Si and high concentration of Al characterized the grains considered to correspond to the sclerotia of Cenoccocum graniforme. The Al-27 MAS NMR spectrum of the sclerotia showed the existence of 6- and 4-coordinated Al. X-ray diffraction analyses supported the presence of amorphous Al (ex. Al-humus complex) in the sclerotia., TAYLOR & FRANCIS LTD
    Soil Science and Plant Nutrition, 2001, [Reviewed]
  • Energy metabolism of Actinobacillus actinomycetemcomitans during anaerobic and micro aerobic growth in low-and high-potassium continuous cultures
    H Ohta; T Inoue; K Fukui, Lead, Actinobacillus actinomycetemcomitans, a member of the gamma subclass of the Proteobacteria, has been implicated as the agent responsible for human periodontitis. In this study, A. actinomycetemcomitans 301-b was grown in fructose-limited chemostat cultures under anaerobic [redox potential (E-h) < -400 mV] and microaerobic (E-h = -200 mV) conditions to characterize its energy metabolism. Effects of K+ and Na+ on growth and metabolism were also examined. In a control medium containing 5(.)2 mM K+ and 24 mM Na+, the molar growth yield on fructose (Y-fructose) of microaerobic cultures was 1(.)3 times higher than the yield of anaerobic cultures at D less than or equal to 0(.)10 h(-1), but the difference in the Y-fructose between microaerobic and anaerobic cultures decreased at D > 0(.)10 h(-1). When the ATP yield from fermentation was estimated from the amounts of fructose consumed and acetate formed, the value of the microaerobic culture (2(.)49 mol ATP produced per mol fructose consumed) was lower than the anaerobic value [3(.)13 mol ATP (mol fructose)(-1)]. Therefore, ATP production from fermentation could not account for the increase in the Y-fructose at D less than or equal to 0(.)10 h(-1) and thus additional ATP was expected to be generated via respiration. Assuming that the Y-ATP (g cells formed per mol ATP synthesized) was similar between anaerobic and microaerobic cultures, the estimated ATP yield from respiration was between 1(.)2 and 2(.)0 mol ATP (mol fructose)(-1) below D = 0(.)10 h(-1) and decreased to 0(.)3 mol ATP (mol fructose)(-1) when D was increased to 0(.)19 h(-1). Such growth-rate-dependent decreases in the Y-fructose and the estimated ATP production from respiration were also observed in a high-Na+ (5(.)2 mM K+ and 106 mM Na+) culture but not in a high-K+ (81 mM K+ and 24 mM Na+) culture. In the high-K+ culture, the microaerobic Y-fructose was 1(.)4-2(.)0 times higher than the anaerobic value and the respiration-derived ATP yield was estimated to be between 1(.)2 and 1(.)9 mol ATP (mol fructose)(-1) over a wide range of dilution rate. These results suggest that higher concentrations of extracellular K+ are required for the respiration to occur in rapidly growing cells of A. actinomycetemcomitans., SOC GENERAL MICROBIOLOGY
    Microbiology, 2001, [Reviewed]
  • Kinetic analysis of ferulic acid degradation by oligotrophic Sphingomonas sp. S213 during growth in batch and continuous cultures
    Hiroyuki Ohta, Lead, Sphingomonas sp. S213 is a soil oligotrophic, Gram-negative bacterium capable of degrading lignin-related ferulic acid. In this study, the capacity of strain S213 to degrade ferulic acid was characterized from its growth parameters in batch and continuous cultures and the first steps in the degradation pathway were identified. Batch cultures with different concentrations of ferulate (0-9.8 mM) showed a profile of substrate inhibition of growth
    i.e. the growth rate increased with ferulate concentration up to an optimum value (0.15-0.3 mM), after which the inhibitory effect became dominant. In contrast, the ferulate degradation rate decreased progressively with increasing concentration. HPLC analysis and oxygen uptake experiments on resting cell suspensions indicated that the first steps in ferulate degradation involve the conversion to vanillate followed by demethylation to protocatechuate, then ring fission of protocatechuate. In chemostat cultures, the specific rates for converting ferulate to vanillate and for converting vanillate to protocatechuate were a linear function of the growth rate. At growth rates above 0.044 h-1 (medium with 0.02% yeast extract) or 0.054 h-1 (with 0.05% yeast extract), the increase in the rate for converting ferulate to vanillate was not accompanied by a proportional increase in the rate for converting vanillate to protocatechuate. This implied that strain S213 had an overcapacity for the conversion of ferulate to vanillate during ferulate metabolism. © 2001, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2001, [Reviewed]
  • Organic nutrient-dependent degradation of branched nonylphenol by Sphingomonas sp. YT isolated from a river sediment sample
    De Vries; Y.P.; Y. Takahara; Y. Ikunaga; Y. Ushiba; M. Hasegawa; Y. Kasahara; H. Shimomura; S. Hayashi; Y. Hirai; H. Ohta, Corresponding, A conventional enrichment culture on branched nonylphenol (NP) with diluted nutrient broth as an additional source of organic nutrients yielded a bacterial strain able to degrade branched NP. The isolate (designated YT) was identified as Sphingomonas sp. based on an analysis of its 16S ribosomal RNA genes and cellular lipids. The degradation of NP by strain YT occurred primarily during the exponential phase of cell growth in cultures on a yeast extract-mineral salts medium. The degree of degradation was directly proportional to the amount of yeast extract present in the medium and no significant growth occurred when NP was the sole source of carbon and energy. Gas chromatography-mass spectrometry (GC-MS) of resting cell suspensions incubated with branched NP revealed that the degradation did not yield any metabolites containing aromatic residues but only branched alcohols. When a linear NP was used as the target substrate, GS-MS of the suspensions indicated the appearance of a hydroxylated linear NP as an intermediate during the degradation. Strain YT is expected to attack NP by an initial oxidative cleavage of the phenol ring. © 2001, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2001, [Reviewed]
  • Nucleotide sequencing and transcriptional analysis of two tandem genes encoding glucosyltransferase(water-soluble-glucan synthetase)in Streptococcus cricetus HS-6
    M Inoue; T Inoue; A Miyagi; Tanimoto, I; R Shingaki; H Ohta; K Fukui, Two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (GTF-S) were cloned from the lambda gene library of Streptococcus cricetus HS-6 (serotype a) using anti-GTF-S antibody, and the nucleotide sequences were analyzed, The two genes (ORF1 and ORF2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and C-terminal repeated units for dextran binding, and [2] a homology search revealed that the ORF1 and ORF2 products are homologous to the GtfS protein (77.4 %) of S, downei Mfe28 and GtfT protein (83.8 %) of S, sobrinus OMZ176, respectively, which are both known to have GTF-S activity. Therefore, ORF1 and ORF2 might be designated gtfS and gtfT of S. criceas, respectively, A Northern blotting and RNase protection assay suggested that the gtfS and gtfT of S, criceas are transcribed as a single bicistronic mRNA as well as separate monocistronic mRNAs, Primer extension analysis indicated multiple transcriptional start points for each gene., CENTER ACADEMIC PUBL JAPAN
    Microbiology and Immunology, 2000, [Reviewed]
  • Endophytic bacterial population in Philippine sugarcane cultivars and isolation of nitrogen-fixing strains
    Constancio A. Asis; Masatsugu Kubota; Vladimir K. Chebotar; Hiroyuki Ohta; Yasuhiro Arima; Koushi Nishiyama; Ken-Ichi Tsuchiya; Shoichiro Akao, The number of endophytic bacteria in the stem of sugarcane cultivars collected from different sites in the Philippines ranged from 104 to 109 cells ml-1 juice. Bacterial population was correlated with the sugar content (% Brix) of the juice and varied depending on the cultivar and sampling site. Twenty-eight isolates had a positive reaction in the acetylene reduction assay (ARA). Presumptive tests indicated that 15 isolates were putative strains of Acetobacter diazotrophicus, 5 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 8 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in sugarcane cultivars in the Philippines. © 2000, Japanese Society of Microbial Ecology &
    The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2000, [Reviewed]
  • Isolation and partial characterization of endophytic diazotrophs associated with Japanese sugarcane cultivar
    CA Asis; M Kubota; H Ohta; Y Arima; VK Chebotar; K Tsuchiya; S Akao, Endophytic bacteria were isolated from the juice of a 6 month-old sugarcane cv. NiF-8, which was collected from Miyako, Okinawa and planted in a field lysimeter in Tsukuba, Ibaraki, Japan. The most probable number of N-2-fixing endophytes was 4.5X10(5) cells per gram of fresh weight sample and 21 isolates have a positive reaction for ARA in an N-free semi-solid medium with 10% crystallized sugarcane sugar and 0.5% sugarcane juice. Analyses of some of the biochemical properties of the N-2-fixing isolates indicated that 13 isolates were putative strains of Acetobacter diazotrophicus, 4 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 4 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N-2-fixing endophytic bacteria in the Japanese sugarcane cv. NiF-8., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Science and Plant Nutrition, 2000, [Reviewed]
  • Growth characteristics of Agromonas oligotrophica on ferulic acid
    Hiroyuki Ohta, Lead, Agromonas oligotrophica is an oligotrophic, dinitrogen-fixing soil bacterium which is found to grow on ferulic acid. This study aimed to characterize in more detail the growth of A. Oligotrophica on ferulate and the degradation of ferulate. Ten- and 100-fold dilutions of conventional nutrient broth (NB/10 and NB/100, respectively) and yeast extract-supplemented mineral salts (MS) media were used for the batch growth experiments. In these media, the initial growth of A. Oligotrophica was suppressed by ferulate in a dose-dependent manner. However, the cell biomass formed in the cultures was positively correlated with the amounts of ferulate used at ferulate concentrations between 0 and 5 mM, indicating that ferulate was the growth-limiting nutrient under the conditions. Thus, the growth yield on ferulate was calculated to be 59-67 g dry weight (mol ferulate)-1 in the NB/10 and the yeast extract-supplemented MS cultures, while in the NB/100 cultures a lower value [33 g dry weight (mol ferulate)-1] was obtained. In a resting cell experiment with ferulate, vanillate was identified by high performance liquid chromatography and the degradation pathway was expected to be analogous to the well-known pathway which includes vanillate and protocatechuate as intermediates. © 2000, Japanese Society of Microbial Ecology – The Japanese Society of Soil Microbiology. All rights reserved.
    Microbes and Environments, 2000, [Reviewed]
  • Heterogeneous post-translational modification of Actinobacillus actinomycetemcomitans fimbrillin
    T Inoue; H Ohta; Tanimoto, I; R Shingaki; K Fukui, Fresh isolates of Actinobacillus actinomycetemcomitans produce bundle-forming fimbriae, The exact molecular mass of A. actinomycetemcomitans fimbrillin, a structural subunit of fimbriae, was determined by liquid ionization mass spectrometry, Three major molecular species with 6,226.0, 6,366.0, and 6,513.0 Da were detected in a purified fimbrial fraction from the strain 310-a. These molecular masses were significantly higher than the molecular weight (5,118 Da) calculated from nucleotide sequence data of the fimbrillin gene, flp, suggesting that the fimbrial peptides were post-translationally modified. Modification of the fimbrial peptides was also suggested by an N-terminal amino acid sequence analysis of fimbrillin peptic fragments, with the modified amino acids being due to seven serine or asparagine residues located in the C-terminal region. A periodate oxidation/biotin-hydrazide labeling assay of fimbrillin suggested that it might be glycosylated., CENTER ACADEMIC PUBL JAPAN
    Microbiology and Immunology, 2000, [Reviewed]
  • Cell surface-associated enolase in Actinobacillus actinomycetemcomitans
    H Hara; H Ohta; T Inoue; T Ohashi; S Takashiba; Y Murayama; K Fukui, Corresponding, Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI, The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase, Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity, In contrast, the relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant, These results suggest the external localization of enolase in this bacterium., CENTER ACADEMIC PUBL JAPAN
    Microbiology and Immunology, 2000, [Reviewed]
  • Cytotoxic necrotizing factor type 2 produced by pathogenic Escherichia coli deamidates a Gln residue in the conserved G-3 domain of the Rho-family and preferentially inhibits the GTP ase activity of Rho A and Rac1
    M Sugai; K Hatazaki; A Mogami; H Ohta; SY Peres; F Herault; Y Horiguchi; M Masuda; Y Ueno; H Komatsuzawa; H Suginaka; E Oswald, Cytotoxic necrotizing factor types 1 and 2 (CNF1 and -2) produced by pathogenic Escherichia coli strains have 90% conserved residues over 1,014-amino-acid sequences. Both CNFs are able to provoke a remarkable increase in F-actin structures in cultured cells and covalently modify the RhoA small GTPases. In this study, we demonstrated that CNF2 reduced RhoA GTPase activity in the presence and absence of P122(RhoGAP). Subsequently, peptide mapping and amino acid sequencing of CNF2-modified FLAG-RhoA produced in E, coli revealed that CNF2 deamidates Q63 of RhoA-like CNF1. In vitro incubation of the C-terminal domain of CNF2 with FLAG-RhoA resulted also in deamidation of the FLAG-Rhoil suggesting that this region contains the enzymatic domain of CNF2. An oligopeptide antibody (anti-E63) which specifically recognized the altered G-3 domain of the Rho family reacted wtith glutathione S-transferase (GST)-RhoA and GST-Rac1 but not with GST-Cdc42 when coexpressed with CNF2. In addition, CNF2 selectively induced accumulation of GTP form of FLAG-RhoA and FLAG-Rac1 but not of FLAG-Cdc42 in Cos-7 cells. Taken together, these results indicate that CNF72 preferentially deamidates RhoA Q63 and Rad Q61 and constitutively activates these small GTPases in cultured cells. In contrast, anti-E63 reacted with GST-RhoA and GST-Cdc42 but not with GST-Rac1 when coexpressed with CNF1, These results indicate that CNF2 and CNF1 share the same catalytic activity but have distinct substrate specificities, which may reflect their differences in toxic activity in vivo., AMER SOC MICROBIOLOGY
    Infection and Immunity, 1999, [Reviewed]
  • Structure and enzymatic properties of genetically truncated forms of the water-insoluble glucan-synthesizing glucosyltransfevase from Streptococcus sobrinus
    N Konishi; Y Torii; T Yamamoto; A Miyagi; H Ohta; K Fukui; S Hanamoto; H Matsuno; H Komatsu; T Kodama; E Katayama, Glucosyltransferase-I (GTF-I: 175 kDa) of a cariogenic bacterium, Streptococcus sobrinus 6715, mediates the conversion of water-soluble dextran (alpha-1,6-glucan) into a water-insoluble form by making numerous alpha-1,3-glucan branches along the dextran chains with sucrose as the glucosyl donor, The structures and catalytic properties were compared for two GTF-I fragments, GTF-I' (138 kDa) and GS (110 kDa). Both lack the N-terminal 84 residues of GTF-I. While GTF-I' still contains four of the six C-terminal repeats characteristic of streptococcal glucosyltransferases, GS lacks all of them, Electron microscopy of negatively stained samples indicated a double-domain structure for GTF-I', consisting of a spherical head with a smaller spherical tail, which was occasionally seen as a long extension. GS was seen just as the head portion of GTF-I', In the absence of dextran, both fragments simply hydrolyzed sucrose with similar K-m and k(cat) values at low concentrations (<5 mM), At higher sucrose concentrations (>10 mM), however, GTF-I' exhibited glucosyl transfer activity to form insoluble alpha-1,3-glucans. So did GS, but less efficiently. Dextran increased the rate and efficiency of the glucosyl transfer by GTF-I'. On removal of the C-terminal repeats of GTF-I' by mild trypsin treatment, this dextran-stimulated transfer was completely lost and the dextran-independent transfer became less efficient. These results indicate that the N-terminal two-thirds of the GTF-I sequence are organized as a structurally and functionally independent domain to catalyze not only sucrose hydrolysis but also glucosyl transfer to form alpha-1,3-glucan chains, although not efficiently; the C-terminal repeat increases the efficiency of the intrinsic glucosyl transfer by the N-terminal domain as well as rendering the whole molecule primer-dependent for far more efficient insoluble glucan synthesis., JAPANESE BIOCHEMICAL SOC
    Journal of Biochemistry, 1999, [Reviewed]
  • Isolation of MHC class I-restricted tumor antigen peptide and its precursors associated with heat shock protein hsp 70, hsp 90, and gp 96
    T Ishii; H Udono; T Yamano; H Ohta; A Uenaka; T Ono; A Hizuta; N Tanaka; PK Srivastava; E Nakayama, We have previously demonstrated that vaccination with heat shock proteins hsp70, hsp90, and gp96 elicits specific immunity against the tumor from which the hsps were purified. Although the association of tumor Ag peptides with these hsps have been suggested, the identification of the peptides or their precursors stripped from the hsps remained to be resolved. We show in this report that an L-d-restricted cytotoxic T lymphocyte epitope of a mouse leukemia RL male 1 and its precursors are associated with the chaperones hsp90 and hsp70 in the cytosol and gp96 in the lumen of the endoplasmic reticulum, Hsp70 was associated,vith only final sized octamer, while hsp90 was found to associate with the octamer and two distinct precursor peptides, The gp96,vas associated with the octamer and one of the two precursors. Thus, each of the hsps bound a distinct set of peptides, Our results have demonstrated for the first time that the hsps associate not only with final sized tumor Ag peptide but also with its precursors. The implication of this evidence is also discussed in terms of the roles of hsps in MHC class I Ag processing/presentation., AMER ASSOC IMMUNOLOGISTS
    The Journal of Immunology, 1999, [Reviewed]
  • Molecular characterization of low-molecular-weight component protein, Flp, in Actinobacillus actinomycetemcomitans fimbriae
    T Inoue; Tanimoto, I; H Ohta; K Kato; Y Murayama; K Fukui, Fimbriae preparation from Actinobacillus actinomycetemcomitans was found to contain an abundant low-molecular-weight protein (termed Flp) with an apparent molecular mass of approximately 6.5 kDa, in addition to a small amount of 54-kDa protein. Immunogold electron microscopy localized the Flp protein at the bacterial fimbriae but not at the cell surface. The DNA fragment including the flp gene was cloned from A. actinomycetemcomitans 304-a and its nucleotide sequence was determined. An open reading frame of the flp gene was composed of 225 bp encoding a protein of 75 amino acids. Comparison of the translated amino acid sequence with the sequence of native Flp determined by Edman degradation indicated that the N-terminal part of 26 amino acids is leader peptide. The N-terminal sequence of mature Flp exhibited some similarity to type-IV pilin, Furthermore, the processing site of premature Flp is also similar to that of type-IV prepilin, and a gene encoding a protein homologous to type-IV prepilin-like protein leader peptidase was found downstream of the flp gene. These findings indicate that Flp is the major component protein of A. actinomycetemcomitans fimbriae., CENTER ACADEMIC PUBL JAPAN
    Microbiology and Immunology, 1998, [Reviewed]
  • The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans
    K Mizoguchi; H Ohta; A Miyagi; H Kurihara; S Takashiba; K Kato; Y Murayama; K Fukui, Corresponding, The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A. actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h(-1) and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, alpha-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain., ELSEVIER SCIENCE BV
    FEMS Microbiology Letters, 1997, [Reviewed]
  • Microaerophilic property of Actinobacillus actinomycetemcomitans in fructose-limited chemostat cultures
    Hiroyuki Ohta; Daisuke Moriki; Atsushi Miyagi; Tatsuo Watanabe; Keijiro Kato; Kazuhiro Fukui, Lead, The effect of oxygen on the growth, metabolism, and leukotoxin production of Actinobacillus actinomycetemcomitans 301-b was examined using a chemostat equipped with a redox potential control system. Steady states were obtained with fructose-limited cultures grown at a dilution rate of 0.1 h(-1) under strictly anaerobic (E(h) = -460 mV) and microaerobic conditions (E(h) less than or equal to -150 mV) but not under highly aerated conditions (E(h) greater than or equal to -100 mV). The optimum growth was recorded at E(h) = -300 to -200 mV and the recorded Y-fructose value was about 1.3 times the Y-fructose of anaerobic cultures. Although the organism contains a respiratory chain, the increased Y-fructose under the microaerobic conditions might result from the increased substrate-level phosphorylation at the site of acetate kinase but not from electron transport phosphorylation. After passing threshold aeration (E(h) = -100 mV), the culture yielded a variant with transparent colony morphology. Under anaerobic conditions, the Y-fructose of the variant was about 1.6 times that of the original opaque colony-forming cells. The optimum growth of the variant was also recorded at E(h) = -300 to -200 mV. In both types of cells, the production of leukotoxin reached a maximum at E(h) = -350 to -200 mV. These findings suggested the microaerophilic nature of A. actinomycetemcomitans., ELSEVIER SCIENCE BV
    FEMS Microbiology Letters, 1996, [Reviewed]
  • The relationships between leukotoxin production, growth rate and the bicarbonate concentration in a toxin-production-variable strain of Actinobacillus actinomycetemlonitans
    H Ohta; A Miyagi; K Kato; K Fukui, Lead, Actinobacillus actinomycetemcomitans. a Gram-negative periodontopathic bacterium, produces a leukotoxin belonging to the RTX family. The production of leukotoxin varies greatly among different strains of this species. In this paper the effects of growth rate and bicarbonate on the leukotoxin production by a toxin-production-variable strain (301-b) during growth in a chemostat were examined. When the bacterium was grown in anaerobic fructose-limited chemostat cultures (ph 7.0 and 37 degrees C) at dilution rates (D) ranging from 0.04 to 0.20 h(-1) in the absence and presence of 10 mM bicarbonate, it produced leukotoxin as a cluster of two polypeptides (M(r) 113000 and 120000) and complexed with nucleic acids on the bacterial cell surface. The relationship between leukotoxin production and specific growth rate was analysed by plotting the specific rate of leukotoxin production [q(LT), in mu g (mg dry wt)(-1) h(-1)] against D. The plots were approximated to the linear relationships q(LT) = 2.7D - 0.058 and q(LT) = 9.3D - 0.407 without and with bicarbonate, respectively. These relationships suggest that the apparent leukotoxin production is a result of both growth-rate-dependent production and growth-rate-independent decomposition. The cellular leukotoxin level was also followed after the change from chemostat to batch culture in the same fermenter. In batch culture leukotoxin production stopped immediately and the cellular toxin level rapidly decreased, suggesting toxin decomposition. From the slopes of the approximated linear relationships between q(LT) and D, a theoretical maximum leukotoxin yield (Y-LT) was estimated as 2.7 and 9.3 mu g (mg dry wt)(-1) in the absence and presence of 10 mM bicarbonate, respectively. The increased Y-LT value in the cultures containing bicarbonate indicated that the addition stimulated the efficiency of leukotoxin synthesis up to about threefold. Further increases of bicarbonate concentration to between 20 and 40 mM had no effect on the total leukotoxin production, but the amount of extracellular leukotoxin increased with higher bicarbonate concentrations., SOC GENERAL MICROBIOLOGY
    Microbiology, 1996, [Reviewed]
  • Novel FNR homologues identified in four representutive oral facaltutive anaerobes : Capnocytophaga ochracea, Capnocytophaga spatigena, Haemophilus aphrophilus, and Actinobacillus actinomycetem comitans
    T Hattori; K Takahashi; T Nakanishi; H Ohta; K Fukui; S Taniguchi; M Takigawa, Based upon DNA sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the FNR family were identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans. The similarity to E. coli FNR and to HlyX (itself 71% similar to E. coli FNR, while regulating expression of hemolysin operon in Actinobacillus pleuropneumoniae) was estimated from the deduced partial amino acid sequence to be, in the above order of tested species, 98, 98, 86, and 85%, and 75, 75, 88, and 88%, respectively. The phylogenetic relatedness indicates a rather closer link of HlyX to the FNR homologues from both pathogens, H. aphrophilus and A. actinomycetemcomitans. The possibility that the A. actinomycetemcomitans FNR homologue functions as a redox-sensing transcriptional factor to regulate, in addition to anaerobic respiration, microaerobic expression of the leukotoxin operon (Itx gene) is suggested., ELSEVIER SCIENCE BV
    FEMS Microbidogy Letters, 1996, [Reviewed]
  • Distribution of black-pigmented Prevotella and Porphyromonas species in the dentition of moderate periodontitis patients
    Osamu Isoshiam; Hiroyuki Ohta; Hidemi Kurihara; Keijiro Kato; Kazuhiro Fukui; Yoji Murayama, The distribution of Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella denticola, Prevotella intermedia, Prevotella loescheii, and Prevotella melaninogenica in periodontally healthy and diseased sites of patients was investigated. The periodontal pockets of two moderate periodontitis patients were scored clinically and sampled microbiologically at their first visit to Okayama University Dental Hospital. A total of 427 isolates of black-pigmented, anaerobic gram-negative rods (BPARs) were identified using a quick method of DNA-DNA hybridisation. In both subjects, all six BPAR species examined were distributed in clusters in certain areas of the dentition. P. gingivalis and Pr. intermedia were the most prevalent species identified. The DNA fingerprinting analysis of P. gingivalis isolates demonstrated that such a cluster was composed of one or two clonal types of the same species, and that five clonal types of the species were present in the whole dentition. For one subject, clinical and microbiological examinations were performed again 3 months later. P. gingivalis and PI. inter-media were still prevalent and distributed in clusters. Among the five preexisting clonal types of P. gingivalis, four were still found, and six other types were recognised. Site-to-site and time-to-time variations in the species profile of BPAR and the clonal profile of P. gingivalis in individual sites may explain the uneven progression of disease in the dentition., JOHN WILEY & SONS LTD
    Microbial Ecology in Health and Disease, 1995, [Reviewed]
  • Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238
    Manabu Miyamoto; Yoshitomo Kobayashi; Susumu Kokeguchi; Hiroyuki Ohta; Hidemi Kurihara; Kazuhiro Fukui; Yoji Murayama, A genomic DNA library of Campylobacter rectus (Wolinella recta) ATCC 33238 was constructed using bacteriophage lambda EMBL3 as a vector. One clone expressing the S-laver protein was identified immunologically with the antiserum to the S-layer protein of C. rectus ATCC 33238. Western immunoblotting using monoclonal antibodies directed against the S-layer protein showed a single blot of recombinant protein at 150 kDa, suggesting that this clone contained the entire coding region of the S-layer protein gene. Additionally, the S-layer protein gene was subcloned into plasmid vector pUC18. Southern hybridization revealed that the S-layer protein gene was present on the chromosome of C. rectus as a single-copy gene, and that there were minor heterogeneities among the S-layer protein genes of clinical isolates. Moreover, a spontaneous stable mutant strain, which has no S-layer expression, also conserved the full-length gene of the S-layer protein., ELSEVIER SCIENCE BV
    FEMS Microbiol. Lett, 1994, [Reviewed]
  • Metabolic and energetic aspects of the growth response of Streptococcus rattus to environmental acidification in anaerobic continuous culture
    Atsushi Miyagi; Hiroyuki Ohta; Takao Kodama; Kazuhiro Fukui; Keijiro Kato; Tsutomu Shimono, Streptococcus rattus. a serotype b strain of mutans streptococci, was grown in an anaerobic glucose-limited chemostat. The molar growth yield of glucose [Y-glucose, g dry wt (mol glucose)(-1)] together with the maximum growth yields (Y-max) and maintenance coefficients for glucose utilization and calculated ATP generation were estimated as a function of pH. When the pH was lowered from 7.0 to 5.0, Y-glucose decreased, with a concomitant gradual change in the composition of the end product from a mixture of formate, acetate and ethanol to one mostly of lactate. Whereas the Y-max for glucose decreased without any change in the Y-max for ATP on acidification, both of the maintenance coefficients markedly increased. Kinetic and immunochemical examinations indicated the presence of an F1F0-type proton-translocating ATPase in the membrane fraction prepared from bacterial cells grown under acidic conditions; no detectable level of the enzyme was found in cells grown at neutral pH. However, when incubated with glucose under non-growing conditions, these acid-adapted and unadapted cells showed an insignificant difference in the ability to maintain the intracellular pH alkaline relative to the acidic environments. These results suggest that the organism responds and adapts to environmental acidification by sacrificing some energy cost in terms of both the efficiency of glucose utilization to generate ATP and the extra maintenance required to continue biomass production as efficiently as under neutral pH., SOC GENERAL MICROBIOLOGY
    Microbiology, 1994, [Reviewed]
  • Antigenic properties of Campylobacter rectus (Wolinella recta) major S-layer proteins
    Yoshitomo Kobayashi; Hiroyuki Ohta; Susumu Kokeguchi; Yoji Murayama; Keijiro Kato; Hidemi Kurihara; Kazuhiro Fukui, The antigenic properties of the surface layer (S-layer) proteins of various Campylobacter rectus strains including 24 clinical isolates and the type strain ATCC 33238 were examined. S-layer proteins were extracted from whole cells by acid treatment according to the method of McCoy et al. (Infect. Immun. 11, 517-525, 1975). The acid extracts from 23 of the isolates and ATCC 33238 contained two major proteins with molecular masses of 130 kDa and 150 kDa, both of which were identified as subunits of the S-layer after comparison with the protein profiles of acid-treated (S-layer-deficient) cells. An S-layer protein from one isolate (CI-808) demonstrated a different molecular mass (160 kDa). Both the 150-kDa proteins of ATCC 33238 and isolate CI-306 and the 160-kDa protein of CI-808 were purified by ion-exchange chromatography in the presence of urea. In Ouchterlony immunodiffusion experiments with these purified proteins and rabbit antiserum raised to each purified protein, both common and strain-specific antigenic determinants were identified in the C. rectus S-layer proteins., ELSEVIER SCIENCE BV
    FEMS Microbiol. Lett, 1993, [Reviewed]
  • Effect of Ca2+ on the binding of Actinobacillus actinomycetemcomitans leukotoxin and the cytotoxicity to promyelocytic leukemia HL-60 cells
    Nobuhiko Sato; koujiro Takahashi; Hiroyuki Ohta; Hidemi Kurihara; Kazuhiro Fukui; Yoji Murayama; Shigehiko Taniguchi, ACADEMIC PRESS AUST
    Biochem. Mol. Biol. Int, 1993, [Reviewed]
  • Association of Actinobacillus actinomycetemcomitans leukotoxin with nucleic acids on the bacterial cell surface
    Hiroyuki Ohta; Hideaki Hara; Kazuhiro Fukui; Hidemi Kurihara; Yoji Murayama; Keijiro Kato, Lead, Actinobacillus actinomycetemcomitans, a periodontopathic gram-negative bacterium, produces a leukotoxin that is a member of the RTX cytotoxin family. Although genes may function in toxin secretion, the leukotoxin is not secreted extracellularly but remains associated with the bacterial cell surface. We report here that this toxin-cell surface association is mediated by nucleic acids and directly demonstrate that the extracellular secretion of toxin occurs in growing cultures with increased ionic strength of medium. All examinations were performed with freshly harvested A. actinomycetemcomitans 301-b from anaerobic fructose-limited chemostat cultures. The occurrence of cell surface-localized DNA was shown by directly digesting whole cells with the restriction endonuclease EcoRI or HindIII, which yielded many DNA fragments. The cell surface DNA constituted about 20% of the total cellular DNA. The leukotoxin was released from the whole cells by digestion with DNase I as well as restriction endonucleases. Because the leukotoxin binds ionically to DNA, it is dependent on the ionic strength of buffers or media. Accordingly, the toxin was released from cells suspended in saline at pH 7.5 in the presence of increasing amounts of MgCl2 (0 to 10 mM) or NaCl (0 to 50 mM). Moreover, a considerable quantity of leukotoxin was detected in the culture supernatant of fructose-limited chemostat cultures when sodium succinate solution was pumped into the steady state as an additional salt (30 and then 50 mM). This toxin-DNA association was also found in well-characterized strains including not only the leukotoxin-producing ATCC 29522 but also the toxin production-variable ATCC 29523 and the non-leukotoxin-producing ATCC 33384 when these strains were grown in the chemostat culture., AMER SOC MICROBIOLOGY
    Infection and Immunity, 1993, [Reviewed]
  • Sts1, a new Fok1 isoschizomer from Streptococcus sanguis 54, cleaves 5' GGATG(N)10/14 3'
    Keiko Kita; H. Kotani; Hiroyuki Ohta; H. Yanase; N. Kato, OXFORD UNIV PRESS UNITED KINGDOM
    Nucleic Acids Res, 1992, [Reviewed]
  • 口腔細菌の嫌気的呼吸系発現に係わるFNR様転写制御蛋白質
    高橋浩二郎; 服部高子; 太田寛行; 野地澄晴; 加藤慶二郎; 谷口茂彦
    Jpn. J. Oral Biol., 1992, [Reviewed]
  • Microbial interactions and the development of periodontal disease
    Hiroyuki Ohta; Keijiro Kato; Kazuhiro Fukui; Jan C. Gottschal, Lead
    Journal of Periodontal Research, 1991, [Reviewed]
  • Peptide sequences for sucrose splitting and glucan binding within Streptococcus sobrinus glucosyltransferase (water-insoluble glucan synthetase)
    Hirokazu Abo; T. Matsumura; Takao Kodama; Hiroyuki Ohta; Kazuhiro Fukui; Keijiro Kato; H. Kagawa, The gene encoding glucosyltransferase responsible for water-insoluble glucan synthesis (GTF-I) of Streptococcus sobrinus (formerly mutans 6715) was cloned, expressed, and sequenced. A gene bank from S. sobrinus 6715 DNA was constructed in vector pUC18 and screened with anti-GTF-I antibody to detect clones producing GTF-I peptide. Five immunopositive clones were isolated, all of which produced peptides that bound-alpha-1, 6 glucan. GTF-I activity was found in only two large peptides: one stretching over the full length of the GTF-I peptide and composed of about 1,600 amino acid residues (AB1 clone) and the other lacking about 80 N-terminal residues and about 260 C-terminal residues (AB2 clone). A deletion study of the AB2 clone indicated that specific glucan binding, which is essential for water-insoluble glucan synthesis, was lost prior to sucrase activity with an increase in deletion from the 3' end of the GTF-I gene. These results suggest that the GTF-I peptide consists of three segments: that for sucrose splitting (approximately 1,100 residues), that for glucan binding (approximately 240 residues), and that of unknown function (approximately 260 residues), in order from the N terminus. The primary structure of the GTF-I peptide, deduced by DNA sequencing of the AB1 clone, was found to be very similar to that of the homologous protein from another strain of S. sorbrinus., AMER SOC MICROBIOLOGY
    J. Bacteriol., 1991, [Reviewed]
  • Nuclease-sensitive binding of an Actinobacillus actinomycetemcomitans leukotoxin to the bacterial cell surface
    Hiroyuki Ohta; Keijiro Kato; Susumu Kokeguchi; Hiroaki Hara; Kazuhiro Fukui; Yoji Murayama, Lead, A leukotoxin of Actinobacillus actinomycetemcomitans 301-b was solubilized from cell-associated membrane vesicles by treatment with externally added DNase and RNase and was further purified by a procedure which included ammonium sulfate fractionation, gel filtration chromatography, and ion-exchange chromatography. The purified toxin had a molecular mass of 113,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a high isoelectric point (approximately 8.8). From these characteristics, it was to be expected that the membrane vesicle toxin was almost identical to the leukotoxin extracted with polymyxin B in an earlier study (C.-C. Tsai, B. J. Shenker, J. M. DiRienzo, D. Malamud, and N. S. Taichman, Infect. Immun. 43:700-705, 1984). The treatment with DNase and RNase was also highly effective for solubilizing the leukotoxin directly from whole cells, suggesting that the toxin is secreted extracellularly but retained in nucleic acids on the outermost surface of bacterial cells., AMER SOC MICROBIOLOGY
    Infection and Immunity, 1991, [Reviewed]
  • Aspartate and asparagine as electron acceptors for Wolinella recta
    Hiroyuki Ohta; Jan C. Gottschal; Kazuhiro Fukui; Keijiro Kato, Lead, Since fumarate and nitrate are not usually available in the oral ecosystem, it was investigated whether aspartate and asparagine could be used as alternative electron acceptors by Wolinella recta, which is strictly dependent on a respiratory metabolism with formate or H-2 as electron donors. Both aspartate and asparagine were indeed shown to support growth of W. recta with formate as electron donor. Fermentative growth with aspartate alone was not possible. Succinate was the major end-product and was formed in equimolar quantities with respect to the amount of formate consumed. The consumption of aspartate and asparagine, on a molar basis, was 10-30% higher than that of formate. Cell-free extracts were prepared from cells grown with formate + fumarate, formate + aspartate, formate + asparagine, and formate + fumarate + aspartate. All these extracts contained high activities of asparaginase, aspartate ammonia-lyase and fumarate-reductase, but no significant activity of aspartate aminotransferase was detected, indicating that fumarate was synthesized directly from aspartate and subsequently reduced to succinate. Based on these results it seems likely that aspartate and asparagine can serve as natural electron acceptors for W. recta in periodontal lesions in which proteolytic bacteria abound., MUNKSGAARD INT PUBL LTD
    Oral Microbiology and Immunology, 1991, [Reviewed]
  • Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans
    Tetsuyoshi Inouye; Hiroyuki Ohta; Susumu Kokeguchi; Kazuhiro Fukui; Keijiro Kato, ELSEVIER SCIENCE BV
    FEMS Microbiol. Lett, 1990, [Reviewed]
  • Streptococcus mutansの増殖に及ぼす酸素の影響               
    嶋本達家; 福井一博; 児玉孝雄; 下野 勉; 太田寛行; 加藤慶二郎
    Jpn. J. Oral Biol, 1990, [Reviewed]
  • Interrelationships between Wolinella recta and Streptococcus sanguis in mixed continuous cultures
    Hiroyuki Ohta; Jan C. Gottschal; Kazuhiro Fukui; Keijiro Kato, Lead, Mixed cultures of Wolinella recta, an asaccharolytic formate-oxidising bacterium, and Streptococcus sanguis, a saccharolytic formate-producing bacterium, were studied in chemostats with glucose as the limiting nutrient and fumarate and/or oxygen as an electron acceptor. Steady-state cultures were established at different levels of fumarate and air supply. Two different types of relationships between both species were recognised in such cultures. A commensal relationship, with W.recta growing at the expense of formate produced by S.sanguis, was observed in strictly anaerobic cultures with fumarate as the sole electron acceptor. A distinctly mutualistic relationship was established in aerated cultures. In these cultures W.recta scavenged oxygen (or possibly H2O2 produced by S.sanguis) whereas S.sanguis provided W.recta with formate through the fermentation of glucose. The ecological consequences for the role of W.recta in the changeover from a healthy to a diseased subgingival microflora is discussed. ©1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted., Informa Healthcare
    Microb. Ecol. Health Dis, 1990, [Reviewed]
  • Effect of bicarbonate on the growth of Actinobacillus actinomycetemcomitans in anaerobic fructose-limited chemostat culture
    Hiroyuki Ohta; Kazuhiro Fukui; Keijiro Kato, Lead, SOC GENERAL MICROBIOLOGY
    J. Gen. Microbiol., 1989, [Reviewed]
  • ATP formation in aerobic catabolism of ethanol by oral streptococci
    Fukui; K.; Kato; K.; Kodama; T.; Ohta; H.; Shimamoto; T. and Shimono; T., JAPAN ACAD
    Proc. Japan Acad., 1988, [Reviewed]
  • Microaerophilic growth of Wolinella recta ATCC33238
    Ohta; H. and Gottschal; J.C., Lead, ELSEVIER SCIENCE BV
    FEMS Microbiol. Ecol, 1988, [Reviewed]
  • Formate oxidation by Wolinella recta ATCC33238 with oxygen as electron acceptor
    Ohta; H. and Gottschal; J.C., Lead, ELSEVIER SCIENCE BV
    FEMS Microbiol. Lett., 1988, [Reviewed]
  • Kinetic study of a change in intracellular ATP level associated with aerobic catabolism of ethanol by Streptococcus mutans
    Fukui; K.; Kato; K.; Kodama; T.; Ohta; H.; Shimamoto; T. and Shimono; T., AMER SOC MICROBIOLOGY
    J. Bacteriol, 1988, [Reviewed]
  • Respiratory characteristics of two oligotrophic bacteria: Agromonas oligotrophica JCM 1494 and Aeromonas hydrophila 315
    Ohta; H. and Taniguchi; S., Lead, MICROBIOL RES FOUNDATION
    J. Gen. Appl. Microbiol., 1988, [Reviewed]
  • Growth characteristics of the soil oligotrophic bacterium: Agromonas oligotrophica JCM1494 on diluted nutrient broth.
    Ohta; H. and Taniguchi; S., Lead, MICROBIOL RES FOUNDATION
    J. Gen. Appl. Microbiol., 1988, [Reviewed]
  • Amphipathic antigen from Eubacterium nodatum
    Kokeguchi; S. Ohta; H.; Fukui; K.; Kato; K. and Tsujimoto, ELSEVIER SCIENCE BV
    FEMS Microbiol. Lett., 1987, [Reviewed]
  • Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae
    Kokeguchi; S. Kato; K.; Ohta; H.; Fukui; K.; Tsujimoto; M.; Ogawa; T.; Takeda; H. and Kotani; S., FACULTY PRESS
    Microbios, 1987, [Reviewed]
  • Leukotoxic activity in Actinobacillus (Haemophilus) actinomycetemcomitans isolated from periodontal disease patients
    Ohta; H.; Kokeguchi; S.; Fukui; K. and Kato. K., Lead, CENTER ACADEMIC PUBL JAPAN
    Microbiol. Immunol., 1987, [Reviewed]
  • Effects of oxygen on glucose-limited growth of Streptococcus mutans
    Kodama; T.; Fukui; K.; Shimamoto; T.; Ohta; H.; Kokeguchi; S. and Kato; K., AMER SOC MICROBIOLOGY
    Infect. Immun., 1987, [Reviewed]
  • 早期発症型歯周炎を伴った母娘症例の細菌叢、ならびに宿主防御機能に関する研究
    者, Early-onset periodontitis has been implicated from the perspective of a host-parasite interaction. This case report describes microbiological examinations and the assessment of host defence function which were performed on a mother and daughter both of whom had early-onset periodontitis. The mother's diagnosis was rapidly progressive periodontitis. She had severe gingival inflammation and progressive bone destraction. The daughter's diagnosis was localized juvenile periodontitis. She had little gingival inflammation, but presented with progressive bone resorption. Microbiological examinations revealed an elevated proportion of rods and spirochetes from the mother's affected sites. Fewer spirochetes were found in the daughter's affected sites. Fusobacterium sp. were the predominant microorganisms in the plaque of both the mother and the daughter. Actinobacillus actinomycetemcomitans was not detected in the subgingival plaque of either the mother or the daughter. Humoral immune responces both in mother and daughter were much higher to F. nucleatum B. gingival, and A. actinomycetemcomitans than any other periodontally related microorganisms examined. Peripheral neutrophil functions were also studied. These studies demonstrated that the mother had a depressive neutrophil phagocytosis. The neutrophil studies performed for the daughter revealed a depressed function not only in neutrophil phagocytosis but also in neutrophil chemotaxis. From the perspective of host-parasite interaction, we propose that the disease status of the mother and the daughter are very similar. We believe the disease process in both the mother and the daughter strongly suggests an identical mechanism., The Japanese Society of Periodontology
    日本歯周病学会会誌, 1987, [Reviewed]
  • Actinobacillus (Haemophilus) actinomycetemcomitans in periodontal disease
    Ohta; H. Kokeguchi; S.; Fukui; K. and Kato; K., Lead, CENTER ACADEMIC PUBL JAPAN
    Microbiol. Immunol., 1986, [Reviewed]
  • Oligotrophic bacteria on organic debris and plant roots in a paddy field soil
    Ohta; H. and Hattori; T., Lead, PERGAMON-ELSEVIER SCIENCE LTD
    Soil Biol. Biochem., 1983, [Reviewed]
  • Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium
    Ohta; H. and Hattori; T., Lead, KLUWER ACADEMIC PUBL
    Antonie van Leeuwenhoek, 1983, [Reviewed]
  • Nitrogen fixation by oligotrophic bacteria on the flood rice roots
    Ohta; H. and Hattori; T., Lead, TAYLOR & FRANCIS LTD
    Soil Sci. Plant Nutr., 1983, [Reviewed]
  • Characterization of halo- and organo-sensitive bacteria from the natural environments               
    Ohta; H. and Hattori.; T., Lead
    Verh. Internat. Verein. Limnol., 1982, [Reviewed]
  • Bacteria sensitive to nutrient broth medium in terrestrial environments
    Ohta; H. and Hattori; T., Lead, JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
    Soil Sci. Plant Nutr., 1980, [Reviewed]

MISC

Books and other publications

  • 現場が動き出す大学教育のマネジメントとは               
    「茨城大学コミットメント」プロジェクト, Compilation
    技術評論社, 11 May 2023
    9784297135096
  • 土壌学概論               
    太田寛行 他, Contributor
    朝倉書店, Aug. 2018, [Reviewed]
    9784254435719
  • 農学が世界を救う! 食料・生命・環境をめぐる科学の挑戦               
    生源寺眞一; 太田寛行; 安田弘法, Joint editor
    岩波ジュニア新書, 20 Oct. 2017
    9784005008612
  • 難培養微生物研究の最前線III               
    太田寛行; 佐藤嘉則; 西澤智康, Joint work
    シーエムシー出版, 07 Aug. 2015
  • 微生物の地球化学               
    太田寛行; 難波謙二; 諏訪裕一; 片山葉子, Joint work
    東海大学出版部, 30 Jun. 2015
    9784486020707
  • 農学入門-食料・生命・環境科学の魅力-               
    太田寛行; 成澤才彦, Joint work
    養賢堂, 20 Sep. 2013
    9784842505190
  • Designing Our Future: Local Perspectives on Bioproduction, Ecosystems and Humanity, 2-1 Sustainable agriculture practices               
    Komatsuzaki; M.; H. Ohta, Joint work
    United Nations University Press, 2011
    9789280811834
  • Establishing a Resource-Circulating Society in Asia: Challenges and Opportunities               
    Niita; Y.; H. Ohta; and T. Kato, Joint work
    United Nations University Press, 2011
    9789280811841
  • 書名,「通信」の時代と現在の間;担当章タイトル,栗原康先生の「生態学的種」をめぐって:1965年からの展開を考える               
    太田寛行, Joint work
    博友社, 10 May 2010
  • 書名、「サステイナビリティ学をつくる」:担当項タイトル、「地球環境を支える微生物たち」               
    三村信男; 伊藤哲司; 田村誠; 佐藤嘉則; 編集; 太田寛行他著, Joint work
    新曜社, 05 Jun. 2008
  • 微生物生態学入門(日本微生物生態学会教育研究部会編)               
    太田寛行他, Joint work
    日科技連, Sep. 2004
  • 新・土の微生物(10)研究の歩みと展望               
    太田寛行, Joint work
    博友社, 2003
  • 土壌微生物生態学               
    Joint work
    朝倉書店, 2003
  • Metro Manila: In Search of a Sustainable Future, Impact analysis of metropolitanpolicies for development and environmental conservation               
    Watanabe; M.; M. Yoshida; H. Ohta; et al., Joint work
    University of the Philippines Press, 2002
  • 自然力を知る-ピナシボ火山災害地域の環境再生-               
    吉田正夫; 太田寛行, Joint work
    古今書院, 2002
  • 新・土の微生物(5)系統分類からみた土の細菌               
    太田寛行他, Joint work
    博友社, 2000
  • バイオフィルムーその生成メカニズムと防止のサイエンスー               
    太田寛行; 鳥井康弘, Joint work
    サイエンスフォーラム, 1998
  • う蝕細菌の分子生物学:研究の成果と展望               
    宮城淳; 太田寛行; 福井一博, Joint work
    クインテッセンス出版, 1997
  • 新土壌微生物実験法               
    太田寛行他, Joint work
    養賢堂, 1992
  • 歯周治療の科学               
    太田寛行; 加藤慶二郎, Joint work
    医歯薬出版, 1991
  • Periodontal disease: pathogens & host immune responses               
    Ohta; H.; Kato; K.; et al., Joint work
    Quintessence Publishing Co., Ltd., 1991
  • 微生物学ハンドブック               
    太田寛行; 鈴木健一朗; 渡辺信; 島津 昭 他, Joint work
    丸善, 1990
  • 微生物の生態(6)               
    服部 勉; 服部黎子; 太田寛行; 塩田悠賀里, Joint work
    学会出版センター, 1979

Lectures, oral presentations, etc.

  • 24 継続的な不耕起農法によって形成される土壌団粒の物質循環系メタゲノム解析(関東支部講演会)
    浦本 匠; 郭 永; 迫田 翠; 坂上 伸生; 小松﨑 将一; 太田 寛行; 西澤 智康
    日本土壌肥料学会講演要旨集, 04 Sep. 2023, 一般社団法人 日本土壌肥料学会
    20230904, 20230904
  • P5-1-1 三宅島2000年噴火後の植生回復過程における土壌の有機・無機成分の変化について(5-1 土壌生成・分類 2023年度愛媛大会)
    竹内 琴音; 川崎 愛希; 坂上 伸生; 西澤 智康; 太田 寛行
    日本土壌肥料学会講演要旨集, 04 Sep. 2023, 一般社団法人 日本土壌肥料学会
    20230904, 20230904
  • 29 三宅島2000年噴火後の植生回復過程における土壌生成と有機物の集積(関東支部講演会)
    竹内 琴音; 宇賀神 温; 西澤 智康; 太田 寛行; 坂上 伸生
    日本土壌肥料学会講演要旨集, 04 Sep. 2023, 一般社団法人 日本土壌肥料学会
    20230904, 20230904
  • 三宅島2000年噴火後の植生回復過程における土壌生成と有機物の集積               
    竹内琴音; 宇賀神温; 西澤智康; 太田寛行; 坂上伸生
    日本土壌肥料学会講演要旨集(Web), 2023
    2023, 2023
  • 三宅島2000年噴火後の植生回復過程における土壌の有機・無機成分の変化について               
    竹内琴音; 川崎愛希; 坂上伸生; 坂上伸生; 西澤智康; 西澤智康; 太田寛行
    日本土壌肥料学会講演要旨集(Web), 2023
    2023, 2023
  • 1 不耕起畑地に形成される土壌団粒が土壌窒素循環に及ぼす影響(関東支部講演会 2021年度支部講演会)
    浦本 匠; 坂上 伸生; 迫田 翠; 郭 永; 小松﨑 将一; 太田 寛行; 西澤 智康
    日本土壌肥料学会講演要旨集, 05 Sep. 2022, 一般社団法人 日本土壌肥料学会
    20220905, 20220905
  • 三宅島火山灰堆積物において窒素循環を駆動する微生物群集のメタゲノミクス               
    宇賀神温・郭永・坂上伸生・上條隆志・太田寛行・西澤智康
    日本土壌微生物学会2022 年度大会, 18 Jun. 2022, 日本土壌微生物学会
    20220618, 20220619
  • 不耕起畑地に形成される土壌団粒が土壌窒素循環に及ぼす影響               
    浦本 匠・坂上 伸生・迫田 翠・郭 永・小松﨑 将一・太田 寛行・西澤 智康
    2021 年度日本土壌肥料学会関東支部大会, 18 Nov. 2021, 日本土壌肥料学会関東支部大会
    20211118, 20211120
  • 三宅島 2000 年火山灰堆積物における微生物生態系解析:20 年間の遷移               
    宇賀神 温・坂上伸生・上條隆志・太田寛行・西澤智康
    日本土壌微生物学会2021年度大会, 18 Jun. 2021, 日本土壌微生物学会
    20210618, 20210619
  • 三宅島2000年噴火から20年間の生態系モニタリング:2000年噴火火山灰中の微生物群集の構造と動態変化               
    太田寛行、西澤智康
    日本生態学会第68回全国大会, 17 Mar. 2021, 日本生態学会, [Invited]
    20210317, 20210321
  • 三宅島2000年火山灰堆積物における微生物生態系解析:20年間の遷移               
    宇賀神温; 坂上伸生; 上條隆志; 太田寛行; 西澤智康
    日本土壌微生物学会大会講演要旨集(CD-ROM), 2021
    2021, 2021
  • CLPP解析に基づく環境保全型畑地土壌の炭素利用性               
    阿蘇日和、荒木肇、小松崎将一、太田寛行、西澤 智康
    日本土壌肥料学会2020年度岡山大会, 08 Sep. 2020, 日本土壌肥料学会
    20200908, 20200910
  • 3-1-10 CLPP解析に基づく環境保全型畑地土壌の炭素利用性(3-1 土壌生物の生態と機能 2020年度岡山大会)
    阿蘇 日和; 荒木 肇; 小松崎 将一; 太田 寛行; 西澤 智康
    日本土壌肥料学会講演要旨集, 01 Sep. 2020, 一般社団法人 日本土壌肥料学会
    20200901, 20200901
  • Soil aggregate structure and soil processes II
    Arai Miwa; Tago Kanako; Wagai Rota; Toride Nobuo; Aoyama Masakazu; Nishizawa Tomoyasu; Kaneko Nobuhiro; Matsuoka Kensuke; Ohta Hiroyuki
    Japanese Journal of Soil Science and Plant Nutrition, 2020, Japanese Society of Soil Science and Plant Nutrition
    2020, 2020
  • カバークロップ施用畑地土壌における土壌団粒微生物の動態解析               
    阿蘇日和、中根麻冴美、荒木肇、小松崎将一、太田寛行、西澤智康
    日本土壌肥料学会2019年静岡大会, 05 Sep. 2019, 日本土壌肥料学会
    20190903, 20190905
  • P3-1-2 カバークロップ施用畑地土壌における土壌団粒微生物の動態解析(3-1 土壌生物の生態と機能,2019年静岡大会)
    阿蘇 日和; 中根 麻冴美; 荒木 肇; 小松崎 将一; 太田 寛行; 西澤 智康
    日本土壌肥料学会講演要旨集, 03 Sep. 2019, 一般社団法人 日本土壌肥料学会
    20190903, 20190903
  • Veronaeopsis simplex Y34 接種トマトの根圏微生物叢の経時的モニタリング               
    野口 愛、高田圭太、橋本実佳、松下紗季、坂上伸生、郭 永、西澤智康、太田寛行、成澤才彦
    日本土壌微生物学会2019年度大会, 15 Jun. 2019, 日本土壌微生物学会
    20190615, 20190616
  • Mortierella 属糸状菌に内生する Mycoavidus 属細菌のゲノム縮小化               
    郭 永、高島勇介、Dilruba Sharmin、佐藤嘉則、成澤才彦、太田寛行、西澤智康
    日本土壌微生物学会2019年度大会, 15 Jun. 2019, 日本土壌微生物学会
    20190615, 20190616
  • イネ科カバークロップ-不耕起栽培畑地土壌の耐水性団粒構造と微生物叢の解析               
    阿蘇日和、中根麻冴美、荒木 肇、小松崎将一、太田寛行、西澤智康
    日本土壌微生物学会2019年度大会, 15 Jun. 2019, 日本土壌微生物学会
    20190615, 20190616
  • Succession of Betaproteobacteria in the 2010 volcanic deposits of Mt. Merapi, Indonesia               
    Annisa N. Lathifah; Yong Guo; Nobuo Sakagami; Wataru Suda; Masanobu Higuchi; Tomoyasu Nishizawa; Irfan D. Prijambada; Hiroyuki Ohta
    日本土壌微生物学会2019年度大会, 15 Jun. 2019, 日本土壌微生物学会
    20190615, 20190616
  • 高温および有機態窒素条件における Mortierella humilis とその内生細菌がトマト生育に及ぼす影響               
    松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
    日本土壌微生物学会2019年度大会, 15 Jun. 2019, 日本土壌微生物学会
    20190615, 20190616
  • Mortierella humilis/verticillata complexとその内生細菌のトマト生育への影響について               
    松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
    日本微生物生態学会大会(Web), 2019
    2019, 2019
  • 根部エンドファイトVeronaeopsis simplex Y34のトマト根部微生物叢への時空間的な影響               
    野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
    日本微生物生態学会大会(Web), 2019
    2019, 2019
  • 高温および有機態窒素条件におけるMortierella humilisとその内生細菌がトマト生育に及ぼす影響               
    松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
    日本土壌微生物学会講演要旨集, 2019
    2019, 2019
  • Isolation and characterization of endophytic microbes associated with the pioneer grass colonizing on the recent volcanic deposits in the island of Miyake, Japan               
    孫寒; 西澤智康; 成澤才彦; 太田寛行
    日本土壌微生物学会講演要旨集, 2019
    2019, 2019
  • Veronaeopsis simplex Y34接種トマトの根圏微生物叢の経時的モニタリング               
    野口愛; 高田圭太; 橋本実佳; 松下紗季; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
    日本土壌微生物学会講演要旨集, 2019
    2019, 2019
  • Mortierella属糸状菌に内生するMycoavidus属細菌のゲノム縮小化               
    郭永; 高島勇介; SHARMIN Dilruba; 佐藤嘉則; 成澤才彦; 太田寛行; 西澤智康
    日本土壌微生物学会講演要旨集, 2019
    2019, 2019
  • 初成土壌形成と微生物:炭素集積と微生物群集の遷移に関する考察               
    太田寛行、西澤智康、郭 永、平野明則、上條隆志
    日本土壌肥料学会2018年度神奈川大会, 31 Aug. 2018, 日本土壌肥料学会
    20180829, 20180831
  • 三宅島2000年噴火後の土壌層位形成-各層における化学性状の遷移               
    海老原諒子、平野明則、坂上伸生、太田寛行、西澤智康
    日本土壌肥料学会2018年度神奈川大会, 29 Aug. 2018, 日本土壌肥料学会
    20180829, 20180831
  • Molecular characterization of opdA homologs and nmoA genes involved in nonylphenol degradation               
    Mina Ootsuka、Yoko Yoshioka-Ikunaga、Tomoyasu Nishizawa、Morifumi Hasegawa、Yasurou Kurusu、Hiroyuki Ohta
    日本土壌微生物生態学会第32回大会, 11 Jul. 2018, 日本土壌微生物生態学会
    20180711, 20180713
  • 土壌団粒と微生物群集の動態変化:農地土壌管理のインパクトを考える               
    中根麻冴美、雫田麻衣、小松崎将一、橋本知義、西澤智康、太田寛行
    日本土壌微生物学会2018年度大会, 17 Jun. 2018, 日本土壌微生物学会
    20180616, 20180617
  • Veronaeopsis simplex Y34 資材を用いたハウス栽培におけるトマト根部微生物叢への影響               
    野口 愛、高田圭太、坂上伸生、郭 永、西澤智康、太田寛行、成澤才彦
    日本土壌微生物学会2018年度大会, 16 Jun. 2018, 日本土壌微生物学会
    20180616, 20180617
  • 糸状菌内生細菌Mycoavidusの二次代謝産物生合成遺伝子の系統解析               
    郭 永、Dilruba Sharmin、高島勇介、成澤才彦、太田寛行、西澤智康
    日本土壌微生物学会2018年度大会, 16 Jun. 2018, 日本土壌微生物学会
    20180616, 20180617
  • Mortierella 属菌とその内生細菌がトマト生育に及ぼす影響               
    松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
    日本土壌微生物学会2018年度大会, 16 Jun. 2018, 日本土壌微生物学会
    20180616, 20180617
  • 三宅島2000年噴火後の土壌層位形成-各層における化学性状の遷移-               
    海老原諒子; 平野明則; 坂上伸生; 太田寛行; 西澤智康
    日本土壌肥料学会講演要旨集, 2018
    2018, 2018
  • Veronaeopsis simplex Y34資材を用いたハウス栽培におけるトマト根部微生物叢への影響               
    野口愛; 高田圭太; 坂上伸生; 郭永; 西澤智康; 太田寛行; 成澤才彦
    日本土壌微生物学会講演要旨集, 2018
    2018, 2018
  • Mortierella属菌とその内生細菌がトマト生育に及ぼす影響               
    松下紗季; 高島勇介; 西澤智康; 太田寛行; 成澤才彦
    日本土壌微生物学会講演要旨集, 2018
    2018, 2018
  • 不耕起と耕起栽培畑地の耐水性土壌団粒における微生物動態               
    西澤智康、中根麻冴美、雫田麻衣、橋本知義、小松崎将一、太田寛行
    日本土壌肥料学会2017年度仙台大会, 05 Sep. 2017, 日本土壌肥料学会
    20170905, 20170907
  • 不耕起・草生栽培畑地土壌の微生物生態学:耐水性団粒構造と微生物の分布について               
    太田寛行、雫田麻衣、中根麻冴美、西澤智康、橋本知義、西脇淳子、小松崎 将一
    日本土壌肥料学会2017年度仙台大会, 05 Sep. 2017, 日本土壌肥料学会
    20170905, 20170907
  • 三宅島火山灰堆積物に新たに形成された土壌層位のメタゲノム解析               
    平野明則、郭 永、坂上伸生、西澤智康、上條隆志、太田 寛行
    日本土壌肥料学会2017年度仙台大会, 05 Sep. 2017, 日本土壌肥料学会
    20170905, 20170907
  • 三宅島初成土壌に生育するパイオニア植物根圏のニトロゲナーゼ活性と根圏のBurkholderiaceae科細菌               
    海老原諒子、郭 永、西澤智康、上條隆志、太田寛行
    日本土壌肥料学会2017年度仙台大会, 05 Sep. 2017, 日本土壌肥料学会
    20170905, 20170907
  • イネ生育促進効果をもつ脱窒細菌がイネ根域土壌細菌群集に及ぼす影響               
    迫田 翠、水澤舞花、郭 永、塩津文隆、坂上伸生、増冨祐司、太田寛行、藤原 徹、妹尾啓史、西澤智康
    日本土壌肥料学会2017年度仙台大会, 05 Sep. 2017, 日本土壌肥料学会
    20170905, 20170907
  • 土壌層位形成過程や土壌団粒構造の微生物群集構造解析               
    西澤智康、太田寛行
    環境微生物系学会合同大会 2017, 31 Aug. 2017, 日本微生物生態学会、日本土壌微生物学会、環境バイオテクノロジー学会、日本菌学会、,日本微生物資源学会、東北大学大学院生命科学研究科
    20170829, 20170831
  • Comparative analysis of alkylphenol degradation genes in Sphingobium amiense and Sphingobium cloacae               
    Mina Ootsuka; Yoko Yoshioka-Ikunaga; Tomoyasu Nishizawa; Morifumi Hasegawa; Yasurou Kurusu; Hiroyuki Ohta
    環境微生物系学会合同大会 2017, 30 Aug. 2017, 日本微生物生態学会、日本土壌微生物学会、環境バイオテクノロジー学会、日本菌学会、,日本微生物資源学会、東北大学大学院生命科学研究科
    20170829, 20170831
  • Comparative characterization of Arthrobacter strains isolated from the Mt. Merapi and Miyake-jima               
    Annisa Nur Lathifah; Hirano Akinori; Yong Guo; Tomoyasu Nishizawa; Takashi Kamijo; Kazuhiko Narisawa; Irfan D. Prijambada; Hiroyuki Ohta
    環境微生物系学会合同大会 2017, 30 Aug. 2017, 日本微生物生態学会、日本土壌微生物学会、環境バイオテクノロジー学会、日本菌学会、,日本微生物資源学会、東北大学大学院生命科学研究科
    20170829, 20170831
  • Colonization of Burkholderia groups on the rhizosphere of the Miyake-jima pioneer plant               
    Akiko Ebihara; Yong Guo; Takashi Kamijo; Tomoyasu Nishizawa; Hiroyuki Ohta
    環境微生物系学会合同大会 2017, 29 Aug. 2017, 日本微生物生態学会、日本土壌微生物学会、環境バイオテクノロジー学会、日本菌学会、,日本微生物資源学会、東北大学大学院生命科学研究科
    20170829, 20170831
  • 火山遷移は何から始まる?-三宅島における化学合成細菌とその役割-               
    太田寛行
    日本生態学会第64回大会, 15 Mar. 2017, 日本生態学会, [Invited]
    20170314, 20170318
  • 三宅島火山灰堆積物で形成された土壌層位における微生物群集構造解析               
    平野明則、郭 永、西澤智康、上條隆志、成澤才彦、太田 寛行
    日本土壌肥料学会2016年度佐賀大会, 21 Sep. 2016, 日本土壌肥料学会
    20160920, 20160922
  • 自然農法圃場に形成される土壌団粒の組成割合および微生物群集構造解析               
    雫田麻衣、中根麻冴美、橋本知義、小松﨑将一、西脇淳子、太田寛行、西澤智康
    日本土壌肥料学会2016年度佐賀大会, 21 Sep. 2016, 日本土壌肥料学会
    20160920, 20160922
  • Microbial community structure analysis of water-stable soil aggregates in no-till cropping systems               
    Nakane; M.; M. Shizukuda; T. Nishizawa; M. Komatsuzaki; T. Hashimoto; H. Ohta
    XVII International Colloquium on Soil Zoology, 24 Aug. 2016
    20160822, 20160826
  • Molecular Analysis of Microbial Community in Water Stable Aggregation of Upland Field Soils Managed by No-tillage Practices for Seven Years               
    Shizukuda; M.; M. Nakane; T. Nishizawa; M. Komatsuzaki; K. Narisawa; T. Hashimoto; H. Ohta1
    XVII International Colloquium on Soil Zoology, 24 Aug. 2016
    20160822, 20160826
  • 不耕起栽培畑地土壌における耐水性団粒内の微生物群集構造解析               
    中根麻冴美; 雫田麻衣; 西澤智康; 小松崎将一; 橋本知義; 太田寛行
    日本土壌微生物学会2016年度大会, 12 Jun. 2016, 日本土壌微生物学会
  • 糸状菌に内生する細菌の系統と相互作用に関する考察               
    太田寛行; 佐藤嘉則; 西澤智康; 成澤才彦
    日本土壌微生物学会2016年度大会, 11 Jun. 2016, 日本土壌微生物学会
  • Mortierella属糸状菌に内生する細菌の多様性解析               
    生田目光; 高島勇介; 佐藤嘉則; 西澤智康; 成澤才彦; 太田寛行
    日本土壌微生物学会2016年度大会, 11 Jun. 2016, 日本土壌微生物学会
  • Genome analysis of a DSE-commensal bacterium Rhizobium sp. Y9 associated with Veronaeopsis simplex Y34               
    Yong Guo ・Yuuto Matsuoka・Tomoyasu Nishizawa・Hiroyuki Ohta・Kazuhiko Narisawa
    日本土壌微生物学会2016年度大会, 11 Jun. 2016, 日本土壌微生物学会
  • 日本微生物生態学会第30回大会・第7回JTKシンポジウムの報告(大会開催報告)
    太田 寛行; 西澤 智康; 長南 茂
    日本微生物生態学会誌, 2016, 日本微生物生態学会
    2016, 2016
  • 3-1-15 三宅島火山灰堆積物で形成された土壌層位における微生物群集構造解析(3-1 土壌生物の生態と機能,2016年度佐賀大会)
    平野 明則; 郭 永; 西澤 智康; 上條 隆志; 成澤 才彦; 太田 寛行
    日本土壌肥料学会講演要旨集, 2016, 一般社団法人 日本土壌肥料学会
    2016, 2016
  • 3-1-3 脱窒細菌Azoarcus属KH32C株接種による窒素無施肥水田土壌でのイネ生育促進(3-1 土壌生物の生態と機能,2016年度佐賀大会)
    西澤 智康; 水澤 舞花; 塩津 文隆; 作田 庄平; 藤原 徹; 太田 寬行; 妹尾 啓史
    日本土壌肥料学会講演要旨集, 2016, 一般社団法人 日本土壌肥料学会
    2016, 2016
  • The development of soil microbial community during vegetation recovery and pedogenesis on the new volcanic deposits of Miyake-jima               
    GUO Yong; HIRANO Akinori; FUJIMURA Reiko; SATO Yoshinori; NISHIZAWA Tomoyasu; KAMIJO Takashi; NARISAWA Kazuhiko; OHTA Hiroyuki
    日本微生物生態学会第30回大会, 20 Oct. 2015, 日本微生物生態学会
  • Release from catabolite repression and enhancement of butanol production in solventogenic Clostridium beijerinckii SBP2-HB by ammonium acetate               
    Miho Kanemoto; Kent Uehara; Manami Akiyama; Yoshinori Sato; Shigeru Chonan; Youji Nitta; Yasurou Kurusu; Hiroyuki Ohta
    日本微生物生態学会第30回大会, 19 Oct. 2015, 日本微生物生態学会
  • Bacterial community shift along the primary soil horizons on the recent volcanic deposits in the Island of Miyake (Miyake-jima), Japan               
    Akinori Hirano; Yong Guo; Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Takashi Kamijo; Kazuhiko Narisawa; Hiroyuki Ohta
    日本微生物生態学会第30回大会, 19 Oct. 2015, 日本微生物生態学会
  • Detection of uncultured Legionella spp. in cooling tower water samples by amoebic coculturing and quantitative PCR               
    Hiroaki Inoue; Kunio Agata; Hiroyuki Ohta
    日本微生物生態学会第30回大会, 18 Oct. 2015, 日本微生物生態学会
  • 土壌糸状菌に内生するMycophilus cysteinexigensのゲノム解析:内生を確立するメカニズムの推定               
    西澤智康; 藤村玲子; 大島翔子; 大島健志朗; 郭永; 佐藤嘉則; 服部正平; 成澤才彦; 太田寬行
    日本微生物生態学会第30回大会, 18 Oct. 2015, 日本微生物生態学会
  • 不耕起・草生畑地土壌中に形成される土壌団粒の真核微生物群集構造解析               
    雫田麻衣; 中根麻冴美; 西澤智康; 小松﨑将一; 成澤才彦; 橋本知義; 太田寛行
    日本微生物生態学会第30回大会, 18 Oct. 2015, 日本微生物生態学会
  • Ammonium acetate increases the production of butanol by Clostridium beijerinckii cultures growing in sweet sorghum juice               
    Kent Uehara; Manami Akiyama; Miho Kanemoto; Tomoyasu Nishizawa; Shigeru Chohnan; Youji Nitta; Yasurou Kurusu; Hiroyuki Ohta
    日本微生物生態学会第30回大会, 18 Oct. 2015, 日本微生物生態学会
  • 土壌糸状菌に内生するBurkholderiaceae科細菌の比較ゲノム解析               
    西澤智康; 藤村玲子; 大島翔子; 大島健志朗; 郭永; 佐藤嘉則; 服部正平; 成澤才彦; 太田寬行
    日本土壌肥料学会2015年度京都大会, 10 Sep. 2015, 日本土壌肥料学会2015年度京都大会
  • 土壌糸状菌に内生するBurkholderiaceae科細菌の比較ゲノム解析
    西澤智康; 藤村玲子; 大島翔子; 大島健志朗; 郭永; 佐藤嘉則; 服部正平; 服部正平; 成澤才彦; 太田寛行
    日本土壌肥料学会講演要旨集, 09 Sep. 2015
    20150909, 20150909
  • 土壌生成プロセスにおける微生物の役割               
    太田寛行; 西澤智康
    日本土壌微生物学会 2015 年度大会(つくば大会):学会シンポジウム,「国際土壌年-土と微生物を考える」, 22 May 2015, 日本土壌微生物学会, [Invited]
  • 土壌糸状菌 Mortierella elongata に内生する細菌のゲノム特性の解析               
    大島翔子; 藤村玲子; 水上沙紀; 佐藤嘉則; 西澤智康; 成澤才彦; 太田寛行
    日本土壌微生物学会 2015 年度大会(つくば大会), 22 May 2015, 日本土壌微生物学会
  • Characterization of microbial communities in new soil horizons forming on the recent Miyake-jima volcanic deposits               
    郭永; 平野明則; 藤村玲子; 佐藤嘉則; 西澤智康; 上條隆志; 成澤才彦; 太田寛行
    日本土壌微生物学会 2015 年度大会(つくば大会), 22 May 2015, 日本土壌微生物学会
  • 三宅島2000年噴火堆積物中の窒素固定細菌に関する研究               
    平野明則; 郭永; 藤村玲子; 佐藤嘉則; 西澤智康; 上条隆志; 成澤才彦; 太田寛行
    日本土壌微生物学会 2015 年度大会(つくば大会), 22 May 2015, 日本土壌微生物学会
  • 土壌糸状菌Mortierella elongataに内生する細菌のゲノム特性の解析(2015年度大会講演要旨)
    大島 翔子; 藤村 玲子; 水上 沙紀; 佐藤 嘉則; 西澤 智康; 成澤 才彦; 太田 寛行
    土と微生物, 2015, 日本土壌微生物学会
    2015, 2015
  • P25-18 糸状菌Mortierella elongataに内生する新属新種細菌の性状解析(ポスター発表)
    大島,翔子; 佐藤,嘉則; 藤村,玲子; 西村,歩; 西澤,智康; 成澤,才彦; 太田,寛行
    日本微生物生態学会講演要旨集, 16 Oct. 2014, 日本微生物生態学会
    20141016, 20141016
  • 霞ヶ浦流域畑地および林地土壌表層10mレイヤーの薬剤耐性菌の分布(土壌生態系(soil ecosystem),一般講演,環境微生物系学会合同大会2014講演要旨)               
    平野 明則; 上原 研人; 金田一 美有; 郭 永; 西澤 智康; 黒田 久雄; 太田 寛行
    土と微生物, 01 Oct. 2014, 日本土壌微生物学会
    20141001, 20141001
  • Change in rhizosphere microbial community of Miscanthus condensatus, a pioneer plant on recent Miyake-jima volcanic deposit, during the vegetation development(The Joint Meeting of Japanese Environmental Microbiology-related Associations 2014)               
    Guo,Yong; Fujimura,Reiko; Sato,Yoshinori; Nishizawa,Tomoyasu; Kamijo,Takashi; Ohta,Hiroyuki
    土と微生物, Oct. 2014, the Japanese Society of Soil Microbiology
    201410, 201410
  • 土壌細菌の分類および群集解析に関する研究 : H.J. Connの研究からメタゲノミクスへの展開を考える               
    太田寛行
    日本土壌肥料学会2014年度東京大会, 10 Sep. 2014, 日本土壌肥料学会, [Invited]
    20140909, 20140911
  • 3-1-16 Molecular Analysis of Microbial Communities Associated with Roots of Pioneer Grass Miscanthus condensatus on Recent Miyake-jima Volcanic Deposits               
    郭 永; 藤村 玲子; 佐藤 嘉則; 西澤 智康; 上條 隆志; 太田 寛行
    Abstracts of the meeting, the Society of the Science of Soil and Manure, 09 Sep. 2014, Japanese Society of Soil Science and Plant Nutrition
    20140909, 20140909
  • Genomic insights into a novel betaproteobacterium associated endosymbiotically with a fungus Mortierella elongata               
    Shoko Ohshima; Yoshinori Sato; Reiko Fujimura; Ayumu Nishimura; Tomoyasu Nishizawa; Kazuhiko Narisawa; Hiroyuki Ohta
    The 15th International Symposium on Microbial Ecology, 28 Aug. 2014, ISME
  • The abundance and diversity of nitrogen cycling genes in long-term cattle manure slurry-applied upland soils               
    Miyuki Ishikawa; Reiko Fujimura; Hiroshi Niimi; Tomoyoshi Hashimoto; Wataru Suda; Seok-Won Kim; Kenshiro Oshima; Masahira Hattori; Hiroyuki Ohta
    The 15th International Symposium on Microbial Ecology, 26 Aug. 2014, ISME, [Invited]
  • Iron-oxidizing chemolithoautotrophy contributes to the input of C and N from the atmosphere to the early acidic volcanic environment in the Island of Miyake, Japan               
    Hiroyuki Ohta; Reiko Fujimura; Momoko Sato; Yoshinori Sato; Tomoyasu Nishizawa; Takashi Kamijo
    The 15th International Symposium on Microbial Ecology, 26 Aug. 2014, ISME
  • Bacterial and fungal communities associated with roots of a pioneer grass, Miscanthus condensatus, colonized the recent volcanic deposits in the Island of Miyake, Japan               
    Yong Guo; Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Takashi Kamijo; Hiroyuki Ohta
    The 15th International Symposium on Microbial Ecology, 26 Aug. 2014, ISME
  • T-RFLP-based assessment of bacterial community in upland field soils managed by non-tillage and cover-cropping               
    Tomoyasu Nishizawa; Akihiro Kawamura; Masahide Ishii1; Miyuki Ishikawa; Masakazu Komatsuzaki; Yoshinori Sato; Nobuhiro Kaneko; Hiroyuki Ohta
    The 15th International Symposium on Microbial Ecology, 26 Aug. 2014, ISME
  • Metabolic characterization of butanol-producing Clostridium beijerinckii SBP2-HB grown in chemostat culture on glucose and sucrose               
    Miho Kanemoto*1; Manami Akiyama2; M. Habibur Rahman2; Yoshinori Sato; Shigeru Chonan; Youji Nitta; Yasurou Kurusu; Hiroyuki Ohta
    The 15th Internationa Symposium on Microbial Ecology, 25 Aug. 2014, ISME
  • Microbial ecology in early soil development               
    Hiroyuki Ohta; Reiko Fujimura; Yong Guo; Tomoyasu Nishizawa; and Yoshinori Sato
    The 6th Korea-Japan-Taiwan International Symposium on Microbial Ecology, 24 Aug. 2014, The Organizing Committee of the 6th Korea-Japan-Taiwan International Symposium on Microbial Ecology, [Invited]
  • Early microbial succession on volcanic deposits along a vegetation gradient in the Island of Miyake, Japan               
    Hiroyuki Ohta; Yong Guo; Reiko Fujimura; Yoshinori Sato and Takashi Kamijo
    The 6th International Congress of East Asian Federation of Ecological Societies (EAFES), 10 Apr. 2014, East Asian Federation of Ecological Societies (EAFES), [Invited]
  • Root-associated microbial diversity of a pioneer grass, Miscanthus condensatus, on the recent volcanic deposits in the Island of Miyake, Japan               
    Yong Guo; Reiko Fujimura; Yoshinori Sato; Tomoyasu Nishizawa; Takashi Kamijo and Hiroyuki Ohta
    The 6th International Congress of East Asian Federation of Ecological Societies (EAFES), 10 Apr. 2014, East Asian Federation of Ecological Societies (EAFES)
  • P22-18 霞ヶ浦流域畑地および林地土壌表層10mレイヤーの薬剤耐性菌の分布(ポスター発表)
    平野 明則; 上原 研人; 金田一 美有; 郭 永; 西澤 智康; 黒田 久雄; 太田 寛行
    日本微生物生態学会講演要旨集, 2014, 日本微生物生態学会
    2014, 2014
  • 長期スラリー連用畑地土壌の窒素循環遺伝子の解析(2013年度大会一般講演要旨)
    石川 美友紀; 太田 寛行; 藤村 玲子; 新美 洋; 橋本 知義; 奈良岡 雅大; 金錫 元; 須田 亙; 大島 健志朗; 服部 正平
    土と微生物, 2014, 日本土壌微生物学会
    2014, 2014
  • 内生細菌が糸状菌Moritierella elongataの性状に及ぼす影響(2013年度大会一般講演要旨)
    西村 歩; 大島 翔子; 藤村 玲子; 佐藤 嘉則; 大島 健志郎; 服部 正平; 成澤 才彦; 太田 寛行
    土と微生物, 2014, 日本土壌微生物学会
    2014, 2014
  • 畑地への長期的な液状きゅう肥施用がもたらす土壌微生物群集構造の変化
    石川美友紀; 藤村玲子; 新美洋; 橋本知義; 金錫元; 須田亙; 大島健志朗; 服部正平; 太田寛行
    日本微生物生態学会講演要旨集, 17 Nov. 2013
    20131117, 20131117
  • PB-017 Characterization of microbial communities associated with the pioneer plant Miscanthus condensatus colonized the fresh volcanic deposits in the Island of Miyake, Japan               
    Guo,Yong; Fujimura,Reiko; Hosokawa,Ken; Sato,Momoko; Sato,Yoshinori; Nishizawa,Tomoyasu; Kamijo,Takashi; Ohta,Hiroyuki
    日本微生物生態学会講演要旨集, Nov. 2013, Japanese Society of Microbial Ecology
    201311, 201311
  • N2Oアイソトポマーを用いた不耕起畑地圃場からのN2O発生要因の解明
    中島泰弘; 星野(高田)裕子; 佐藤嘉則; 西村歩; 太田寛行; 秋山博子; 早津雅仁; 小松崎将一
    日本土壌肥料学会講演要旨集, 11 Sep. 2013
    20130911, 20130911
  • 水田土壌から分離されたDeinococcus sp. S34株の分類と生態的考察               
    太田寛行; 服部黎子; 三井久幸; 服部 勉
    日本土壌肥料学会2013年度名古屋大会, 11 Sep. 2013, 日本土壌肥料学会
  • 長期スラリー連用畑地土壌の窒素循環遺伝子の解析               
    石川美友紀; 藤村玲子; 新美 洋; 橋本知義; 奈良岡雅大; 金 錫元; 須田 亙; 大島健志朗; 服部正平; 太田寛行
    日本土壌微生物学会2013年度大会, 20 Jun. 2013, 日本土壌微生物学会
  • 混合培養による好気的バイオブタノール生産               
    秋山真成美; 金本美穂; M. habibur Rahman; 佐藤嘉則; 長南 茂; 新田洋司; 久留主泰郎; 太田寛行
    日本土壌微生物学会2013年度大会, 20 Jun. 2013, 日本土壌微生物学会
  • Rhizobiales目細菌の分類学的考察とAlsobacteriaceae fam. nov.の提唱について               
    太田寛行; 包 智華; 藤村玲子; 佐藤嘉則
    日本土壌微生物学会2013年度大会, 20 Jun. 2013, 日本土壌微生物学会
  • 内生細菌が糸状菌Mortierella elongataの性状に及ぼす影響               
    西村 歩; 大島翔子; 藤村玲子; 佐藤嘉則; 大島健志郎; 服部正平; 成澤才彦; 太田寛行
    日本土壌微生物学会2013年度大会, 20 Jun. 2013, 日本土壌微生物学会
  • 長期スラリー連用畑地土壌の窒素循環遺伝子の解析
    石川美友紀; 藤村玲子; 新美洋; 橋本知義; 奈良岡雅大; 金錫元; 須田亙; 大島健志朗; 服部正平; 太田寛行
    日本土壌微生物学会講演要旨集, 19 Jun. 2013
    20130619, 20130619
  • 糸状菌Mortierella elongataの菌糸内部に生息する細菌の全ゲノム解析(最優秀賞,JSME2012豊橋大会特集)
    藤村 玲子; 西村 歩; 佐藤 嘉則; 大島 翔子; 大島 健志朗; 服部 正平; 成澤 才彦; 太田 寛行
    日本微生物生態学会誌, 2013, 日本微生物生態学会
    2013, 2013
  • Succession of microbial ecosystem in fresh volcanic ash deposits on the island Miyake (Miyake-jima), Japan, revealed by metagenomics               
    藤村玲子; 金 錫元; 佐藤 嘉則; 須田 亙; 西澤 智康; 大島 健志朗; 服部 正平; 上條 隆志; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会, [Invited]
  • 霞ヶ浦流域における多剤耐性セラチアとメタロβラクタマーゼの普及               
    今井 達也; M; Habibur Rahman; 黒田 久雄; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • 基質誘導呼吸法を用いた土壌モデル系での異なる農地土壌における真菌類の亜酸化窒素生成活性の比較               
    奈良岡 雅大; 佐藤 嘉則; 新美 洋; 橋本 知義; 石川 美友紀; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • Clostridium beijerinckii SBP2-HB のスイートソルガム搾汁液における発酵特性               
    金本 美穂; 秋山 真成美; Rahman M. Habibur; 佐藤 嘉則; 長南 茂; 新田 洋司; 久留主 泰朗; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • スラリー長期連用圃場における土壌微生物生態系のメタゲノム解析               
    石川 美友紀; 藤村 玲子; 新美 洋; 橋本 知義; 金 錫元; 須田 亙; 大島 健志朗; 服部 正平; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • 糸状菌Mortierella elongata 菌糸内部に生息する細菌のゲノム解析               
    藤村 玲子; 西村 歩; 佐藤 嘉則; 大島 翔子; 大島 健志朗; 服部 正平; 成澤 才彦; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • 三宅島2000 年噴火後の植生回復への菌類エンドファイトの関わりについて               
    臼井 絵里香; 太田 寛行; 成澤 才彦
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • 糸状菌Mortierella elongata の菌糸内部に生息する細菌の除去及び再導入化に関する研究               
    大島 翔子; 佐藤 嘉則; 西村 歩; 藤村 玲子; 木川 りか; 成澤 才彦; 太田 寛行
    第28回日本微生物生態学会大会, 20 Sep. 2012, 日本微生物生態学会
  • S02-1 Succession of microbial ecosystem in fresh volcanic ash deposits on the island of Miyake (Miyake-Jima), Japan, revealed by metagenomics(Session 2 Metagenomic approach to studying soil environmental change,Symposium session)
    Fujimura,Reiko; Kim,Seok-Won; Sato,Yoshinori; Suda,Wataru; Nishizawa,Tomoyasu; Oshima,Kenshiro; Hattori,Masahira; Kamijo,Takashi; Ohta,Hiroyuki
    日本微生物生態学会講演要旨集, 19 Sep. 2012, 日本微生物生態学会
    20120919, 20120919
  • Effect of vegetation cover on bacterial diversity of volcanic deposits in Miyake-jima, Japan               
    Yong Guo; Reiko Fujimura; Yoshinori Sato; Wataru Suwata; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta
    日本土壌肥料学会2012年度鳥取大会, 04 Sep. 2012, 日本土壌肥料学会
  • 土壌水分含量が畑地土壌のin vitro脱窒活性に及ぼす影響の解析               
    太田寛行; 新美 洋; 橋本知義; 梅津昌史; 奈良岡雅大; 佐藤嘉則; 西澤智康; 赤池 恵; 藤村玲子; 小松崎将一; 金子信博
    日本土壌肥料学会2012年度鳥取大会, 04 Sep. 2012, 日本土壌肥料学会
  • Occurrence of multidrug-resistant Serratia spp. in the Kasumigaura Lake drainage basin, Japan               
    Tatsuya Imai; M. Habibur Rahman; Hisao Kuroda; and Hiroyuki Ohta
    14th International Symposium on Microbial Ecology, 23 Aug. 2012, Internationa Society for Microbial Ecology
  • Metagenomic analysis of pioneer microbial community in young volcanic ash deposit on the island of Miyake, Japan.               
    Reiko Fujimura; Seok-Won Kim; Yoshinori Sato; Wataru Suda; Tomoyasu Nishizawa; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo and Hiroyuki Ohta
    14th International Symposium on Microbial Ecology, 21 Aug. 2012, International Society for Microbial Ecology
  • Bacterial community of the fresh volcanic deposits on barren land, grassland, and woodland in the Island of Miyake, Japan, as revealed by pyrosequencing-based analysis of 16S rRNA genes.               
    Yong Guo; Reiko Fujimura; Yoshinori Sato; Kenshiro Oshima; Masahira Hattori; Takashi Kamijo; Hiroyuki Ohta
    14th International Symposium on Microbial Ecology, 21 Aug. 2012, International Society for Microbial Ecology
  • Optimization of culture conditions for the production of butanol from sweet sorghum juice by Clostridium beijerinckii SBP2-HB               
    Miho Kanemoto; M. Habibur Rahman; Yoshinori Sato; Shigeru Chohnan; Youji Nitta; Yasurou Kurusu; and Hiroyuki Ohta
    14th International Symposium on Microbial Ecology, 20 Aug. 2012, International Society for Microbial Ecology
  • P6-8 土壌水分含量が畑地土壌のin vitro脱窒活性に及ぼす影響の解析(S6.N_2O生成と消去に関わる微生物研究の最前線-遺伝子解析からN_2O発生抑制まで-,6.土壌生物,2012年度鳥取大会)
    太田 寛行; 新美 洋; 橋本 知義; 梅津 昌史; 佐藤 嘉則; 西澤 智康; 小松崎 将一; 金子 信博
    日本土壌肥料学会講演要旨集, 2012, 一般社団法人 日本土壌肥料学会
    2012, 2012
  • 土壌モデルを利用した畑地土壌における糸状菌の亜酸化窒素生成の解析               
    奈良岡雅大; 佐藤嘉則; 赤池恵; 太田寛行
    日本土壌微生物学会2011年度大会, 26 Nov. 2011, 日本土壌微生物学会
  • 三宅島2000年噴火堆積物から分離された鉄酸化細菌Leptospirillum ferrooxidans C2-3株のゲノム解析               
    藤村玲子; 佐藤嘉則; 細川研; 西澤智康; 大島健志朗; 服部正平; 上條隆志; 太田寛行
    日本土壌微生物学会2011年度大会, 26 Nov. 2011, 日本土壌微生物学会
  • 不耕起畑地土壌より分離した糸状菌株の亜酸化窒素生成活性               
    赤池 恵; 鶴田和人; 佐藤嘉則; 西村 歩; 小松崎将一; 太田寛行
    日本土壌微生物学会2011年度大会, 26 Nov. 2011, 日本土壌微生物学会
  • 不耕起畑地土壌における微生物多様性の解析-特定サイトでの7年間の調査-               
    河村明寛; 西澤智康; 小松崎将一; 金子信博; 太田寛行
    日本土壌微生物学会2011年度大会, 25 Nov. 2011, 日本土壌微生物学会
  • 三宅島2000年噴火堆積物から分離された鉄酸化細菌Leptospirillum ferrooxidans C2‐3株のゲノム解析
    藤村玲子; 佐藤嘉則; 細川研; 西澤智康; 大島健志朗; 服部正平; 上條隆志; 太田寛行
    土と微生物, 01 Nov. 2011
    20111101, 20111101
  • 微生物多様性研究の現状と可能性:土壌微生物研究からの考察               
    太田寛行; 藤村玲子
    日本微生物生態学会第27回大会, 10 Oct. 2011, 日本微生物生態学会, [Invited]
  • 三宅島2000年噴火火山灰堆積物における初成土壌微生物生態系のメタゲノム解析               
    藤村玲子; 金 錫元; 郭 永; 佐藤嘉則; 細川 研; 須田 亙; 大島健志朗; 服部正平; 上條隆志; 太田寛行
    日本微生物生態学会第27回大会, 09 Oct. 2011, 日本微生物生態学会
  • スイートソルガム搾汁液からブタノールを高生産する細菌株の選抜               
    金本美穂; H.M. Rahman; 佐藤嘉則; 長南 茂; 新田洋司; 久留主泰朗; 太田寛行
    日本微生物生態学会第27回大会, 09 Oct. 2011, 日本微生物生態学会
  • PA-026 基質誘導呼吸阻害法を用いた土壌モデル系での細菌と糸状菌の亜酸化窒素生成活性の解析(PA 土壌生態系,ポスター発表)
    奈良岡,雅大; 佐藤,嘉則; 赤池,恵; 太田,寛行
    日本微生物生態学会講演要旨集, 08 Oct. 2011, 日本微生物生態学会
    20111008, 20111008
  • 三宅島2000年噴火火山灰堆積物における初成土壌微生物態系のメタゲノム解析
    藤村玲子; 金錫元; 敦永; 佐藤嘉則; 細川研; 須田亙; 大島健志朗; 服部正平; 上條隆志; 太田寛行
    日本微生物生態学会講演要旨集, 08 Oct. 2011
    20111008, 20111008
  • 霞ヶ浦流域におけるテトラサイクリン耐性細菌の季節動態               
    今井達也; H.M. Rahman; N.A. Farzana; 黒田久雄; 太田寛行
    日本微生物生態学会第27回大会, 08 Oct. 2011, 日本微生物生態学会
  • 基質誘導呼吸阻害法を用いた土壌モデル系での細菌と糸状菌の亜酸化窒素生成活性の解析               
    奈良岡雅大; 佐藤嘉則; 赤池 恵; 太田寛行
    日本微生物生態学会第27回大会, 08 Oct. 2011, 日本微生物生態学会
  • 有機系銅剤ノニルフェノールスルホン酸銅(NPS-Cu)負荷による土壌細菌/アーキアおよびアンモニア酸化菌の群集構造解析               
    河村明寛; 山田桂衣; 西澤智康; 佐藤嘉則; 太田寛行
    日本微生物生態学会第27回大会, 08 Oct. 2011, 日本微生物生態学会
  • Change in community of associated bacteria with Veronaeopsis simplex in the response of high temperature stress               
    Khastini; R.; Y. Sato; H. Ohta; K. Narisawa
    日本微生物生態学会第27回大会, 08 Oct. 2011, 日本微生物生態学会
  • Metagenomic analysis of microbial succession on fresh volcanic ash deposits in the Island of Miyake, Japan               
    Reiko Fujimura; Seokwon Kim; Yong Guo; Yoshinori Sato; Ken Hosokawa; Wataru Suda; Erika Iioka; Kenshiro Ohshima; Masahira Hattori; Takashi Kamijo and Hiroyuki Ohta
    20th International Symposium on Environmental Biogeochemistry, 28 Sep. 2011, International Society for Environmental Biogeochemistry
  • Biomass circulation system through the biobutanol production from a sugar-rich plant, sweet sorghum               
    Miho Kanemoto; M. Habibur Rahman; Yoshinori Sato; Shigeru Chohnan; Youji Nitta; Yasurou Kurusu; Hiroyuki Ohta
    20th International Symposiun on Environmental Biogeochemistry, 27 Sep. 2011, International Society for Environmental Biogeochemistry
  • Continuous in vitro measurement of soil N2O production in a headspace gas-circulating jar system connected to an infrared gas analyzer               
    Masahiro Naraoka; Yoshinori Sato; Megumi Akaike; Hiroyuki Ohta
    20th International Symposiun on Environmental Biogeochemistry, 27 Sep. 2011, International Society for Environmental Biogeochemistry
  • Distribution and diversity of tetracycline resistant bacteria in the Lake Kasumigaura environment, Japan               
    Tatsuya Imai; M. Habibur Rahman; Hisao Kuroda; and Hiroyuki Ohta
    20th International Symposiun on Environmental Biogeochemistry, 27 Sep. 2011, International Society for Environmental Biogeochemistry
  • 環流土壌系を用いた硝化菌の動態解析:硝化アーキアの働きを考える               
    太田寛行; 山田桂衣; 河村明寛; 安田智昭; 佐藤嘉則; 西澤智康
    日本土壌肥料学会2011年度つくば大会, 09 Aug. 2011, 日本土壌肥料学会
  • Relationship between development of bacterial community and vegetation succession on early soil ecosystem in the Island of Miyake, Japan               
    郭 永; 藤村玲子; 細川 研; 佐藤嘉則; 西澤智康; 上條隆志; 太田寛行
    日本土壌肥料学会2011年度つくば大会, 09 Aug. 2011, 日本土壌肥料学会
  • 6-9 環流土壌系を用いた硝化菌の動態解析 : 硝化アーキアの働きを考える(6.土壌生物)               
    太田 寛行; 山田 桂衣; 河村 明寛; 安田 智昭; 佐藤 嘉則; 西澤 智康
    日本土壌肥料学会講演要旨集, 08 Aug. 2011, 一般社団法人日本土壌肥料学会
    20110808, 20110808
  • 6-31 Relationship between development of bacterial community and vegetation succession on early soil ecosystem in the Island of Miyake, Japan               
    郭, 永; 藤村, 玲子; 細川, 研; 佐藤, 嘉則; 西沢, 智康; 上條, 隆志; 太田, 寛行
    Abstracts of the meeting, the Society of the Science of Soil and Manure, Aug. 2011, Japanese Society of Soil Science and Plant Nutrition
    201108, 201108
  • Distribution of inorganic components of fine sediment of Tone river estuary and their microbial diversity
    SAKAGAMI Nobuo; YAMAZAKI Ken-ichi; SATO Yoshinori; OHTA Hiroyuki; WATANABE Makiko; ISHIKAWA Tadaharu
    Proceedings of the General Meeting of the Association of Japanese Geographers, 2011, The Association of Japanese Geographers
    2011, 2011
  • 不耕起畑地土壌より分離した糸状菌株の亜酸化窒素生成活性(2011年度大会一般講演要旨)
    赤池 恵; 鶴田 和人; 佐藤 嘉則; 西村 歩; 小松崎 将一; 太田 寛行
    土と微生物, 2011, 日本土壌微生物学会
    2011, 2011
  • 土壌モデルを利用した畑地土壌における糸状菌の亜酸化窒素生成の解析(2011年度大会一般講演要旨)
    奈良岡 雅大; 佐藤 嘉則; 赤池 恵; 太田 寛行
    土と微生物, 2011, 日本土壌微生物学会
    2011, 2011
  • 不耕起栽培畑地土壌における微生物多様性の解析 : 特定サイトでの7年間の調査(2011年度大会一般講演要旨)
    河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
    土と微生物, 2011, 日本土壌微生物学会
    2011, 2011
  • 不耕起畑地土壌から分離した糸状菌の亜酸化窒素生成活性               
    赤池 恵; 佐藤嘉則; 西澤智康; 小松崎将一; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • 霞ヶ浦域におけるテトラサイクリン耐性菌の動態               
    今井達也; Rhaman M. Habibur; Neela F. Ashrafi; 黒田久雄; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • 不耕起栽培土壌におけるアーキア群集の分子生態学的解析               
    河村明寛; 西澤智康; 小松崎将一; 金子信博; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • Analysis of bacterial community in early stage of soil ecosystem after the 2000 volcanic eruption in Miyake Island, Japan               
    郭 永; 藤村玲子; 細川 研; 佐藤嘉則; 西澤智康; 上條隆志; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • ノニルフェノールスルホン酸銅環流土壌中における硝化菌の動態解析               
    山田桂衣; 河村明寛; 安田智昭; 佐藤嘉則; 西澤智康; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • スイートソルガム搾汁液からブタノールを生産するクロストリジウム属細菌の増殖特性               
    金本美穂; Rahman Habibur; 長南 茂; 新田洋司; 久留主泰朗; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • 細菌の生態学的種を考える:エコタイプについての考察               
    太田寛行; 西澤智康; 藤村玲子; 佐藤嘉則
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • バングラデシュ・エビ養殖場関連環境中での薬剤耐性菌の動態               
    ニーラ・ファルザナ; ラーマン・アティクル・バヌー; ナアスリンアクター; ラーマン・ハビブル; 太田寛行; アラム・フィロズ
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • 模擬微小重力がPseudomonas aeruginosa株のswarming運動および遺伝子発現に及ぼす影響               
    佐藤慎哉; 西澤智康; 妹尾啓史; 井上哲圭; 太田寛行
    日本微生物生態学会第26回大会, 25 Nov. 2010
  • Characterization of endobacterial communities in dark-septate endophytic fungus, Veronaeopsis simplex associated with biocontrol mechanisms of Fusarium disease of Chinese Cabbage               
    カスチニ・リダ; 佐藤嘉則; 西澤智康; 太田寛行; 成澤才彦
    日本微生物生態学会第26回大会, 24 Nov. 2010
  • 不耕起草生栽培が畑土壌の微生物群集に及ぼす影響               
    西澤智康; 太田寛行
    日本土壌肥料学会2010年度北海道大会, 09 Sep. 2010, 日本土壌肥料学会, [Invited]
  • Characterization of soil microbial community in the early development of soil ecosystem in Miyake Island, Japan               
    郭 永; 藤村玲子; 細川 研; 佐藤嘉則; 西澤智康; 上條隆志; 太田寛行
    日本土壌肥料学会2010年度北海道大会, 07 Sep. 2010, 日本土壌肥料学会
  • P6-1 Characterization of soil microbial community in the early development of soil ecosystem in Miyake Island, Japan               
    郭, 永; 藤村, 玲子; 細川, 研; 佐藤, 嘉則; 西沢, 智康; 上條, 隆志; 太田, 寛行
    Abstracts of the meeting, the Society of the Science of Soil and Manure, Sep. 2010, Japanese Society of Soil Science and Plant Nutrition
    201009, 201009
  • Nitrogen fixation by iron-oxidizing bacteria during early ecosystem development on Miyake-jima, Japan volcanic deposits               
    Fujimura; R.; Y. Sato; T. Sugawara; K. Hosokawa; T. Nishizawa; T. Kamijo and H. Ohta
    13th International Symposium on Microbial Ecology, 27 Aug. 2010, International Society for Microbial Ecology
  • Microbial community structure in volcanic deposits with different succession stages of vegetation in Miyake Island, Japan               
    Guo; Y.; R. Fujimura; K. Hosokawa; Y. Sato; T. Nishizawa; T. Kamijo; and H. Ohta
    13th International Symposium on Microbial Ecology, 26 Aug. 2010, International Society for Microbial Ecology
  • Predomonance of nitrous oxide-producing fungus, Mortierella elongate, in no-tilled, low-Nitrogen input upland soils               
    Sato; Y.; T. Nishizawa; M. Umezu; K. Tsuruta; K. Narisawa; M. Komatsuzaki; H. Ohta
    13th International Symposium on Microbial Ecology, 23 Aug. 2010, International Society for Microbial Ecology
  • Isolation of soil bacteria able to grow in sweet sorghum juice and produce butanol               
    Kanemoto; M.; M. H. Rahman; S. Chohnan; Y. Nitta; Y. Kurusu; H.Ohta
    13th International Symposium on Microbial Ecology, 23 Aug. 2010, International Society for Microbial Ecology
  • Effect of bactericidal copper nonylphenol sulfonate on soil ammonia-oxidizing bacteria and archaea               
    Yasuda; T.; T. Nishizawa; Y. Sato; H. Ohta
    13th International Symposium on Microbial Ecology, 23 Aug. 2010, International Society for Microbial Ecology
  • T-RFLP profiling of soil microbial communities in no-tilled upland soils               
    Kawamura; A.; T. Nishizawa; M. Komatsuzaki; N. Kaneko; H. Ohta
    13th International Symposium on Microbial Ecology, 23 Aug. 2010, International Society for Microbial Ecology
  • スイートソルガム搾汁液からブタノールを生産する細菌の分離と性状解析               
    金本美穂; Habibur Rahman; 長南 茂; 新田洋司; 久留主泰朗; 太田寛行
    日本土壌微生物学会2010年度大会, 22 May 2010, 日本土壌微生物学会
  • 不耕起栽培畑地土壌における微生物群集の分子生態学的解析               
    河村明寛; 西澤智康; 小松崎将一; 金子信博; 太田寛行
    日本土壌微生物学会2010年度大会, 22 May 2010, 日本土壌微生物学会
  • ノニルフェノールスルホン酸銅環流土壌中におけるアンモニア酸化菌の動態解析               
    西澤智康; 安田智昭; 太田寛行
    日本土壌微生物学会2010年度大会, 22 May 2010
  • 畑地土壌におけるCryptococcus属真核微生物の分布と亜酸化窒素生成活性               
    赤池 恵; 佐藤嘉則; 西澤智康; 昭日格図; 太田寛行
    日本土壌微生物学会2010年度大会, 22 May 2010
  • 三宅島火山噴火と土壌微生物生態系の応答               
    太田寛行
    第57回日本生態学会大会, 16 Mar. 2010, 日本生態学会, [Invited]
  • ノニルフェノールスルホン酸銅環流土壌中におけるアンモニア酸化菌の動態解析(2010年度大会一般講演要旨)
    西澤 智康; 安田 智昭; 太田 寛行
    土と微生物, 2010, 日本土壌微生物学会
    2010, 2010
  • P-106 Characterization of clostridia able to grow in sweet sorghum juice and produce butanol(Poster Session)
    KANEMOTO MIHO; HABINUR RAHMAN; CHOHNAN SHIGERU; NITTA YOUJI; KURUSU YASUROU; OHTA HIROYUKI
    日本微生物生態学会講演要旨集, 2010, 日本微生物生態学会
    2010, 2010
  • P-96 Effect of copper nonylphenol sulfonate on ammonia-oxidizing archaea and bacteria in soil percolation column(Poster Session)
    YAMADA KEI; KAWAMURA AKIHIRO; YASUDA TOMOAKI; SATO YOSHINORI; NISHIZAWA TOMOYASU; OHTA HIROYUKI
    日本微生物生態学会講演要旨集, 2010, 日本微生物生態学会
    2010, 2010
  • P-26 Nitrous oxide-producing activity of fungi isolated from no-tilled upland soils(Poster Session)
    AKAIKE MEGUMI; SATO YOSHINORI; NISHIZAWA TOMOYASU; KOMATSUZAKI MASAKAZU; OHTA HIROYUKI
    日本微生物生態学会講演要旨集, 2010, 日本微生物生態学会
    2010, 2010
  • P-85 Analysis of bacterial community in early stage of soil ecosystem after the 2000 volcanic eruption in Miyake Island, Japan(Poster Session) :
    GUO YONG; FUJIMURA REIKO; HOSOKAWA KEN; SATO YOSHINORI; NISHIZAWA TOMOYASU; KAMIJO TAKASHI; OHTA HIROYUKI
    日本微生物生態学会講演要旨集, 2010, 日本微生物生態学会
    2010, 2010
  • 畑地土壌におけるCryptococcus属真核微生物の分布と亜酸化窒素生成活性(2010年度大会一般講演要旨)
    赤池 恵; 佐藤 嘉則; 西澤 智康; 昭日 格図; 太田 寛行
    土と微生物, 2010, 日本土壌微生物学会
    2010, 2010
  • P-76 T-RFLP profiling of soil archaeal community in no-tilled upland soils(Poster Session)
    KAWAMURA AKIHIRO; NISHIZAWA TOMOYASU; KOMATSUZAKI MASAKAZU; KANEKO NOBUHIRO; OHTA HIROYUKI
    日本微生物生態学会講演要旨集, 2010, 日本微生物生態学会
    2010, 2010
  • 不耕起栽培畑地土壌における微生物群集の分子生態学的解析(2010年度大会一般講演要旨)
    河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
    土と微生物, 2010, 日本土壌微生物学会
    2010, 2010
  • ノニルフェノールスルホン酸銅がアンモニア酸化バクテリア・アーキアに与える影響               
    安田智昭; 梅津昌史; 鶴田和人; 西澤智康; 佐藤嘉則; 高原義治; 太田寛行
    日本微生物生態学会第25回大会, 22 Nov. 2009
  • 環境保全型農法と土壌細菌・アーキア群集:ミミズ導入とカバークロップの影響               
    河村明寛; 西澤智康; 小松崎将一; 金子信博; 太田寛行
    日本微生物生態学会第25回大会, 21 Nov. 2009
  • 環境保全型農法を継続した畑地土壌における真核微生物の群集構造解析               
    西澤智康; 河村明寛; 佐藤嘉則; 小松崎将一; 金子信博; 太田寛行
    日本微生物生態学会第25回大会, 21 Nov. 2009
  • 三宅島火山灰堆積物における微生物の鉄代謝               
    細川 研; 郭 永; 藤村玲子; 佐藤嘉則; 西澤智康; 難波健二; 上條隆志; 太田寛行
    日本微生物生態学会第25回大会, 21 Nov. 2009
  • 細菌についての生態学的種を考える:この数年の展開をめぐって               
    太田寛行; 西澤智康; 佐藤嘉則; 藤村玲子
    日本微生物生態学会第25回大会, 21 Nov. 2009
  • 模擬微小重力条件におけるPseudomonas aeruginosaのpilA転写解析               
    佐藤慎哉; 進士真也; 小里郁美; 西澤智康; 井上哲圭; 太田寛行
    日本微生物生態学会第25回大会, 21 Nov. 2009
  • ^<15>N-DNA-SIP法による三宅島火山灰堆積物中の窒素固定微生物の解析(2009年度大会一般講演要旨)               
    菅原, 智詞; 佐藤, 嘉則; 藤村, 玲子; 長澤, 俊樹; 細川, 研; 西澤, 智康; 難波, 謙二; 上條, 隆志; 太田, 寛行
    土と微生物, Oct. 2009, the Japanese Society of Soil Microbiology
    200910, 200910
  • Fungal denitrification and nitrous oxide-producing activities in Japanese upland soils               
    Ohta; H.; M. Umezu; K. Tsuruta; Y. Sato; T. Nishizawa; H. Niimi; T. Hashimoto; K. Narisawa; M. Komatsuzaki
    XIX International Symposium on Environmental Biogeochemistry, 17 Sep. 2009, International Symposium on Environmental Biogeochemistry
  • Identification of intracellular bacteria related to the genus Burkholderia in the mycelia of the nitrous oxide-producing fungus Mortierella elongate               
    Sato; Y.; K. Narisawa; K. Tsuruta; M. Umezu; T. Nishizawa; M. Komatsuzaki; H. Ohta
    XIX International Symposium on Environmental Biogeochemistry, 14 Sep. 2009
  • 15N-DNA-SIP法による三宅島火山灰堆積物中の窒素固定微生物の解析               
    菅原智詞; 佐藤嘉則; 藤村玲子; 長澤俊樹; 細川 研; 西澤智康; 難波謙二; 上條隆志; 太田寛行
    日本土壌微生物学会2009年度大会, 12 Jun. 2009, 日本土壌微生物学会
  • T-RFLPプロファイルによる畑地土壌微生物群集の解析               
    西澤智康; 石井真英; 梅津昌史; 昭日格図; 小松崎将一; 金子信博; 太田寛行
    日本土壌微生物学会2009年度大会, 12 Jun. 2009, 日本土壌微生物学会
  • 糸状菌細胞内生細菌の存在と亜酸化窒素生成活性の関係               
    佐藤嘉則; 鶴田和人; 梅津昌史; 成澤才彦; 太田寛行
    日本土壌微生物学会2009年度大会, 12 Jun. 2009, 日本土壌微生物学会
  • Contribution of Fungi to Nitrous Oxide Emissions from Upland Soils (Japan)               
    Umezu; M.; K. Tsuruta; Y. Sato; T. Nishizawa; H. Niimi; T. Hashimoto; K. Narisawa; M. Komatsuzaki; H. Ohta
    The 1st Korea-Japan Symposium on Microbial Ecology, 28 May 2009, The Microbiological Society of Korea
  • The Motility of Pseudomonas aeruginosa strains on clinorotation               
    Sato; S.; S. Shinji; I. Kozato; T. Nishizawa; T. Inoue; H. Ohta
    The 1st Korea-Japan Symposium on Microbial Ecology, 28 May 2009, The Microbiological Society of Korea
  • The Burkholdseria-related bacteria presented in the mycelia of nitrous oxide-producing fungus, Moritierella elongata               
    Sato; Y.; K. Narisawa; K. Tsuruta; M. Umezu; T. Nishizawa; M. Komatsuzaki; H. Ohta
    The 1st Korea-Japan Symposium on Microbial Ecology, 28 May 2009, The Microbiological Society of Korea
  • P-83 環境保全型農法を継続した畑地土壌における真核微生物の群集構造解析(ポスター発表)
    西澤 智康; 河村 明寛; 佐藤 嘉則; 小松崎 将一; 金子 信博; 太田 寛行
    日本微生物生態学会講演要旨集, 2009, 日本微生物生態学会
    2009, 2009
  • P-63 環境保全型農法と土壌細菌・アーキア群集 : ミミズ導入とカバークロップの影響(ポスター発表)
    河村 明寛; 西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
    日本微生物生態学会講演要旨集, 2009, 日本微生物生態学会
    2009, 2009
  • 1A-17(P-86) 地表部の撹乱が地下圏微生物にどのような影響をおよぼすのか(口頭発表+ポスター発表)
    永翁 一代; 勝山 千恵; 近藤 菜穂; 村上 正志; 西澤 智康; 太田 寛行; 金子 信博; 加藤 憲二
    日本微生物生態学会講演要旨集, 2009, 日本微生物生態学会
    2009, 2009
  • T-RELPプロファイルによる畑地土壌微生物群集の解析(2009年度大会一般講演要旨)
    西澤 智康; 石井 真英; 梅津 昌史; 昭日 格図; 小松崎 将一; 金子 信博; 太田 寛行
    土と微生物, 2009, 日本土壌微生物学会
    2009, 2009
  • 末端制限酵素フラグメント(T-RFs)データベース検索による畑地土壌微生物群集解析               
    西澤智康; 小松﨑将一; 金子信博; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008, 日本微生物生態学会
  • 有機物連用土壌における亜酸化窒素発生に関わる微生物群集構造の解析               
    梅津昌史; 佐藤嘉則; 西澤智康; 新美洋; 橋本知義; 成澤才彦; 小松崎将一; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • T-RFLPインフォマティクスによる畑地土壌細菌群集構造の解析               
    石井真英; 西澤智康; 小松﨑将一; 金子信博; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • SIP法による三宅島火山堆積物中の微生物群衆構造解析               
    菅原智詞; 佐藤嘉則; 藤村玲子; 長澤俊樹; 細川研; 西澤智康; 難波謙二; 上条隆志; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • 不耕起畑地土壌での糸状菌群集と亜酸化窒素生成               
    昭日格図; 西澤智康; 佐藤嘉則; 鶴田和人; 小松崎将一; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008, 日本微生物生態学会
  • 土壌から検出される菌核の微生物担体としての機能               
    野々山弥; 成澤才彦; 太田寛行; 渡邊眞紀子
    日本微生物生態学会第24回大会, 26 Nov. 2008, 日本微生物生態学会
  • クリノスタット条件下でのPseudomonas aeruginosa株の増殖と運動性               
    佐藤慎哉; 小里郁美; 進士信也; 井上哲圭; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008, 日本微生物生態学会
  • 畑地土壌由来糸状菌の分類群と亜酸化窒素生成活性の関係について               
    鶴田和人; 佐藤嘉則; 昭日格図; 成澤才彦; 小松崎将一; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • 三宅島火山灰堆積物中のケモスタット法による鉄酸化細菌の分離               
    細川研; 佐藤嘉則; 菅原智詞; 藤村玲子; 難波謙二; 上條隆志; 西澤智康; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • 糸状菌細胞内生細菌の検出および系統解析               
    佐藤嘉則; 鶴田和人; 梅津昌史; 成澤才彦; 小松崎将一; 太田寛行
    日本微生物生態学会第24回大会, 26 Nov. 2008
  • 種々の窒素化合物培地におけるMetarhizium strain CY-14の亜酸化窒素生成               
    Purba Islam; Y. Sato; Z. Bao; K. Tsruta; M. Umezu; H. Ohta
    日本微生物生態学会第24回大会, 26 Nov. 2008, 日本微生物生態学会
  • Microbial iron cycle accompanied by nitrogen fixation in volcanic ash deposits of Miyake-jima Island, Japan               
    Hiroyuki Ohta; R. Fujimura; Y. Sato; T. Sugawara; K. Hosokawa; T. Nagasawa; K. Nanba; T. Kamijo
    7th International Symposium for Subsurface Microbiology, 20 Nov. 2008, International Society for Subsurface Microbiology
  • Semi quantitative PCR-based T-RFLP profiling of soil microbial communities               
    Tomoyasu Nishizawa; M. Ishii; M. Komatsuzaki; N. Kaneko; H. Ohta
    7th International Symposium for Subsurface Microbiology, 20 Nov. 2008, International Society for Subsurface Microbiology
  • Identification of nitrogen fixing bacteria in recent volcanic deposits of Miyake-jima by stable isotope probing with 15N2               
    Tomonori Sugawara; Y. Sato; R. Fujimura; T. Nagasawa; K. Hosokawa; T. Nishizawa; K.Nanba; T. Kamijo; H. Ohta
    7th International Symposium for Subsurface Microbiology, 20 Nov. 2008, International Society for Subsurface Microbiology
  • 10-198 Identification of nitrogen fixing bacteria in recent volcanic deposits of Miyake-Jima by stable isotope probing(Microbial community analysis)               
    Sugawara,Tomonori; Sato,Yoshinori; Fujimura,Reiko; Nagasawa,Toshiki; Hosokawa,Ken; Nishizawa,Tomoyasu; Nanba,Kenji; Kamijo,Takashi; Ohta,Hiroyuki
    日本微生物生態学会講演要旨集, Nov. 2008, Japanese Society of Microbial Ecology
    200811, 200811
  • 04-078 Isolation of iron-oxidizing bacteria by chemostat from Miyake-jima volcanic ash deposits(Soil ecosystem)               
    Hosokawa,Ken; Sato,Yoshinori; Sugawara,Tomonori; Fujimura,Reiko; Nanba,Kenji; Kamijo,Takashi; Nishizawa,Tomoyasu; Ohta,Hiroyuki
    日本微生物生態学会講演要旨集, Nov. 2008, Japanese Society of Microbial Ecology
    200811, 200811
  • 三宅島2000年噴火堆積物の初成土壌形成と細菌生態系(2008年度大会一般講演要旨)               
    藤村, 玲子; 佐藤, 嘉則; 長澤, 俊樹; 細川, 研; 西澤, 智康; 難波, 謙二; 加藤, 拓; 片山, 葉子; 上條, 隆志; 東, 照雄; 太田, 寛行
    土と微生物, Oct. 2008, the Japanese Society of Soil Microbiology
    200810, 200810
  • T-RFLPデータベースによる畑地土壌細菌群集構造の解析               
    石井真英; 昭日格図; 西澤智康; 小松崎将一; 太田寛行
    日本土壌肥料学会2008年度愛知大会, 10 Sep. 2008, 日本土壌肥料学会
  • 畑地土壌からの亜酸化窒素生成糸状菌の解析               
    鶴田和人; 佐藤嘉則; Puruba Islam; 成澤才彦; 小松崎将一; 太田寛行
    日本土壌肥料学会2008年度愛知大会, 10 Sep. 2008
  • 6-18 畑地土壌からの亜酸化窒素生成糸状菌の解析(6.土壌生物,2008年度愛知大会)               
    鶴田 和人; 佐藤 嘉則; Islaml Puruba; 成澤 才彦; 小松崎 将一; 太田 寛行
    日本土壌肥料学会講演要旨集, 09 Sep. 2008, 一般社団法人日本土壌肥料学会
    20080909, 20080909
  • Effect of bactericidal copper nonylphenol sulfonate on the soil microbial communities               
    Yasuda; T.; M. Umezu; K. Turuta; H. Nishizawa; Y. Sato; Y. Takahara; H. Ohta
    12th International Symposium on Microbial Ecology, 21 Aug. 2008, International Society for Microbial Ecology
  • Relationship between bacterial and fungal denitrification in upland soils: effect of N source and soil moisture               
    Umezu; M.; Y. Sato; T. Nishizawa; H. Niimi; T. Hashimoto; K. Narisawa; M. Komatsuzaki; H. Ohta
    12th International Symposium on Microbial Ecology, 21 Aug. 2008, International Society for Microbial Ecology
  • Profiling of soil archaeal community by real-time quantitative PCR-based T-RFLP               
    Nishizawa; T.; M. Komatsuzaki; N. Kaneko; H. Ohta
    12th International Symposium on Microbial Ecology, 21 Aug. 2008, International Society for Microbial Ecology
  • Detection and identification of endosymbiotic bacteria in the nitrous oxide producing fungus, Mortierella elongata               
    Sato; Y.; K. Tsuruta; M. Umedsu; K. Narisawa; M. Komatsuzaki; H. Ohta
    12th International Symposium on Microbial Ecology, 21 Aug. 2008, International Society for Microbial Ecology
  • Effect of tillage and cover cropping on nitrous oxide emissions and fungal populations in an upland humic allophane soil               
    Zhaorigete; T. Nishizawa; Y. Sato; Y.; M. Komatsuzaki; H. Ohta
    12th International Symposium on Microbial Ecology, 21 Aug. 2008, International Society for Microbial Ecology
  • Microbial ecosystem fixing carbon and nitrogen in fresh volcanic deposit of Miyake-jima Island, Japan               
    Fujimura; R.; Y. Sato; T. Nagasawa; K. Hosokawa; K. Nanba; T. Nishizawa; T. Kamijyo; Y. Katayama; H. Ohta
    12th International Symposium on Microbial Ecology, 18 Aug. 2008, International Society for Microbial Ecology
  • Swarming motility of Pseudomonas aeruginosa strains under the clinorotation               
    Ohta; H.; I. Kozato; S. Shinji; S. Sato; T. Inoue
    12th International Symposium on Microbial Ecology, 18 Aug. 2008, International Society for Microbial Ecology
  • これからが本番の土壌微生物研究 -開会の挨拶をかねて-               
    太田寛行
    第5回澄石セミナー-土壌微生物研究:いま問題にすべきこと、問題にしたいこと-, 21 Jun. 2008
  • 土壌細菌:いま問題にすべきこと、問題にしたいこと               
    太田寛行
    第5回澄石セミナー-土壌微生物研究:いま問題にすべきこと、問題にしたいこと-, 21 Jun. 2008
  • 三宅島2000年噴火堆積物の初成土壌形成と細菌生態系               
    藤村玲子; 佐藤嘉則; 長澤俊樹; 細川研; 西澤智康; 難波謙二; 加藤 拓; 片山葉子; 上條隆志; 東 照雄; 太田寛行
    日本土壌微生物学会2008年度大会, 14 Jun. 2008
  • 土壌微生物群集の定量的T-RFLP解析:窒素負荷量の異なる土壌について               
    梅津昌史; 佐藤嘉則; 西澤智康; 新美 洋; 橋本知義; 小松崎将一; 太田寛行
    日本土壌微生物学会2008年度大会, 13 Jun. 2008
  • T-RFLP解析による土壌微生物群集の評価:ノニルフェノールスルホン酸銅が及ぼす影響               
    安田智昭; 梅津昌史; 鶴田和人; 西澤智康; 佐藤嘉則; 高原義治; 太田寛行
    日本土壌微生物学会2008年度大会, 13 Jun. 2008
  • 不耕起栽培における糸状菌多様性の評価               
    昭日格図; 西澤智康; 佐藤嘉則; 小松崎将一; 太田寛行
    日本土壌微生物学会2008年度大会, 13 Jun. 2008
  • 定量的T-RFLP 解析法による畑地土壌微生物の多様性評価               
    西澤智康; 小松崎将一; 金子信博; 太田寛行
    日本土壌微生物学会2008年度大会, 13 Jun. 2008
  • T-RFLP解析による土壌微生物群集の評価 : ノニルフェノールスルホン酸銅が及ぼす影響(2008年度大会一般講演要旨)
    安田 智昭; 梅津 昌史; 鶴田 和人; 西澤 智康; 佐藤 嘉則; 高原 義治; 太田 寛行
    土と微生物, 2008, 日本土壌微生物学会
    2008, 2008
  • 土壌微生物群集の定量的T-RFLP解析 : 窒素負荷量の異なる土壌について(2008年度大会一般講演要旨)
    梅津 昌史; 佐藤 嘉則; 西澤 智康; 新美 洋; 橋本 知義; 小松崎 将一; 太田 寛行
    土と微生物, 2008, 日本土壌微生物学会
    2008, 2008
  • 不耕起栽培における糸状菌多様性の評価(2008年度大会一般講演要旨)
    昭日 格図; 西澤 智康; 佐藤 嘉則; 小松崎 将一; 太田 寛行
    土と微生物, 2008, 日本土壌微生物学会
    2008, 2008
  • 04-072 Relationship between phylogenetic characteristic and nitrous oxide-producing activity of fungal species isolated from agricultural soils(Soil ecosystem)
    Tsuruta Kazuto; Sato Yoshinori; Jyoru Guto; Narisawa Kazuhiko; Komatsuzaki Masakazu; Ohta Hiroyuki
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 02-033 Fungal community and nitrous oxide emission in no-tillage upland soils(Soil ecosystem)
    Zhaori Getu; Nishizawa Tomoyasu; Sato Yoshinori; Tsuruta Kazuto; Komatsuzaki Masakazu; Ohta Hiroyuki
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 11-211 Function as microbial carrier of sclerotium grains in soil(Soil ecosystem)
    Nonoyama Yaya; Narisawa Kazuhiko; Ohta Hiroyuki; Watanabe Makiko
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 10-195 Effect of bactericidal copper nonylphenol sulfonate on the soil microbial communities : A model using a soil percolation column(Microbial community analysis)
    Yasuda Tomoaki; Umezu Masahumi; Tsuruta Kazuto; Nishizawa Tomoyasu; Sato Yoshinori; Takahara Yoshiharu; Ohta Hiroyuki
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 北海道大学苫小牧研究林における土壌炭素集積速度
    金子信博; 藤巻玲路; 川口達也; 日浦勉; 村上正志; 陀安一郎; 太田寛行; 西沢智康; 増永二之; 岩島範子; 豊田鮎
    第119回日本森林学会大会, 2008
    2008, 2008
  • 11-201 Microbial community analysis of upland field soils by a database search of the terminal-restriction fragments (T-RFs)(Microbial community analysis)
    Nishizawa Tomoyasu; Komatsuzaki Masakazu; Kaneko Nobuhiro; Ohta Hiroyuki
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 06-105 Analysis of upland soil bacterial community by T-RFLP informatics(Microbial community analysis)
    Ishii Masahide; Nishizawa Tomoyasu; Komatsuzaki Masakazu; Kaneko Nobuhiro; Ohta Hiroyuki
    日本微生物生態学会講演要旨集, 2008, 日本微生物生態学会
    2008, 2008
  • 定量的T-RFLP解析法による畑地土壌微生物の多様性評価(2008年度大会一般講演要旨)
    西澤 智康; 小松崎 将一; 金子 信博; 太田 寛行
    土と微生物, 2008, 日本土壌微生物学会
    2008, 2008
  • Contribution of iron-oxidizing bacteria to carbon and nitrogen accumulation in recent volcanic ash deposits of Miyake-jima Island, Japan               
    Fujimura; R.; Y. Sato; K. Nanba; T. Kamijyo; H. Kato; Y. Katayama; H. Ohta
    18th International Symposium on Environmental Biogeochemistry, 12 Nov. 2007
  • 気候変動と微生物生態学ーはじめに               
    太田寛行; 木暮一啓
    日本微生物生態学会第23回大会, 18 Sep. 2007, [Invited]
  • 土壌微生物多様性の環境ゲノミックス解析に向けて               
    妹尾啓史; 太田寛行
    日本微生物生態学会第23回大会, 18 Sep. 2007, [Invited]
  • Effect of copper nonylphenol sulfonate on soil microbial communities               
    Yasuda; T.; M. Umezu; K. Tsuruta; T. Nishizawa; Y. Sato; Y. Takahara; H. Ohta
    日本微生物生態学会第23回大会, 17 Sep. 2007
  • Utilization of phenolic acids by soil bacteria               
    Ohta; H.; K. Nonaka; K. Hosokawa
    日本微生物生態学会第23回大会, 17 Sep. 2007
  • Carbonyl sulfide(COS)-degrading activity and phylogenetic identity of the bacterial isolates from the MPN for counting COS-degrading microbes in soils               
    Kato; H.; Y. Sato; R. Fujimura; H. Ohta; Y. Katayama
    日本微生物生態学会第23回大会, 17 Sep. 2007
  • Trace gas (CO and methane) utilization and tracegas oxidizing bacteria on successional transects of two recent volcanic deposits               
    King; G.M.; C.F. Weber; K. Nanba; H. Ohta
    日本微生物生態学会第23回大会, 17 Sep. 2007
  • Isolation and culturebased and culture-independent analysis of iron-oxidizing bacteria in the Miyake-jima Island deposits               
    Hosokawa; K.; T. Nagasawa; R. Fujimura; Y. Sato; H. Ohta
    日本微生物生態学会第23回大会, 17 Sep. 2007
  • Microbial nitrous oxide emission from agricultural soils under different nitrogen application.               
    Umezu; M.; Y. Sato; H. Niimi; T. Hashimoto; K. Narisawa; M. Komatsuzaki; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Isolation and characterization of Thallium-accumulating soil bacteria               
    Bao; C.; Y. Sato; M. Kubota; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Detection and characterization of endosymbiotic bacteria in the denitrifying fungi               
    Sato; Y.; M. Umezu; K. Tsuruta; K. Narisawa; M. Komatsuzaki; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Denitrification activities of fungal isolates from agricultural soils               
    Tsuruta; K.; Y. Sato; K. Narisawa; M. Komatsuzaki; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Characterization of Limnobacter, Herbaspirillum and Paracoccus strains isolated from volcanic deposits in Miyake-jima Island               
    Lu; H.; R. Fujimura; Y. Sato; T. Kamijyo; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • 不耕起栽培における糸状菌の動態について               
    昭日格図; 西澤智康; 佐藤嘉則; 小松崎将一; 太田寛行
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Diversity of soil microbial communities assessed by PCR-amplified T-RFLP analysis: effects of tillage treatments               
    Nishizawa; T.; M. Komatsuzaki; N. Kaneko; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Developing factors of sclerotia-like structure of some dark septate fungal isolates               
    Nonoyama; Y.; K. Narisawa; H. Ohta; M. Watanabe
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • Growth characteristics of Pseudomonas aeruginosa strains under the clinorotation               
    Kozato; I.; S. Shinji; T. Inoue; H. Ohta
    日本微生物生態学会第23回大会, 16 Sep. 2007
  • 三宅島2000年噴火堆積物中の微生物活性と機能遺伝子の解析               
    佐藤嘉則; 藤村玲子; 難波謙二; 加藤拓; 上條隆志; 東照雄; 太田寛行
    日本土壌肥料学会2007年度東京大会, 24 Aug. 2007
  • 畑地からの糸状菌分離による亜酸化窒素生成活性               
    鶴田和人; 佐藤嘉則; 成澤才彦; 小松崎将一; 太田寛行
    日本土壌肥料学会2007年度東京大会, 24 Aug. 2007
  • 窒素負荷量の異なる畑地土壌における亜酸化窒素生成に関わる微生物の解析               
    梅津昌史; 佐藤嘉則; 新美洋; 橋本知義; 成澤才彦; 小松崎将一; 太田寛行
    日本土壌肥料学会2007年度東京大会, 24 Aug. 2007
  • 6-23 畑地からの糸状菌分離株による亜酸化窒素生成活性(6.土壌生物,2007年度東京大会)               
    鶴田 和人; 佐藤 嘉則; 成澤 才彦; 小松崎 将一; 太田 寛行
    日本土壌肥料学会講演要旨集, 22 Aug. 2007, 一般社団法人日本土壌肥料学会
    20070822, 20070822
  • Pseudomonas putida groupのフェノール性有機酸分解特性と分類学的性状について               
    野中和裕; 細川桂一; 太田寛行
    日本土壌微生物学会2007年度大会, 08 Jun. 2007
  • Characterization of Herbaspirillum spp. isolated from recent volcanic deposits of Miyake-jima Island               
    Lu; H.; Y. Sato; R. Fujimura; H. Ohta
    日本土壌微生物学会2007年度大会, 08 Jun. 2007
  • "Pseudomonas putida group"におけるフェノール性有機酸の分解特性               
    野中和裕; 太田寛行; 細川桂一
    日本微生物生態学会第22回大会, 29 Oct. 2006
  • アーバスキュラー菌根菌胞子および感染根中のポリリン酸の動態               
    西村伊吹; 大友 量; 對馬誠也; 太田寛行; 斎藤雅典
    日本微生物生態学会第22回大会, 29 Oct. 2006
  • Predominance of Limnobacter and Herbaspirillum in recent Miyake-jima volcanic deposits               
    Lu Hongsheng; Yoshinori Sato; Reiko Fujimura; Yoko Katayama; Hiroyuki Ohta
    日本微生物生態学会第22回大会, 29 Oct. 2006
  • Cenococcum属菌の菌核から分離される菌類及び細菌相について               
    野々山弥; 成澤才彦; 太田寛行; 渡邊眞紀子
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • 不耕起栽培土壌の土壌微生物相とカバークロップの効果               
    昭日格図; 小松崎将一; 太田寛行; 佐藤嘉則
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • 畑地土壌における亜酸化窒素の生成に関係する微生物群の分析               
    佐藤嘉則; 梅津昌史; 鶴田和人; 成澤才彦; 小松崎将一; 太田寛行
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • タリウム耐性土壌細菌のタリウムに対する生理特性               
    包 智華; 久保田正亜; 太田寛行
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • クリノスタット条件下での細菌増殖と遺伝子発現               
    小里郁美; 吉村英尚; 井上哲圭; 大森正之; 太田寛行
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • 様々な土壌における従属栄養性の硫化カルボニル分解微生物               
    加藤広海; 佐藤嘉則; 藤村玲子; 太田寛行; 片山葉子
    日本微生物生態学会第22回大会, 28 Oct. 2006
  • Microbial ecology of the Miyake-jima volcanic deposit: lithotrophic microbes and the development of early micro-ecosystem               
    Hiroyuki Ohta; Reiko Fujimura; Yoshinori Sato
    The 4th Okazaki Biology Conference: "Terra Microbiology 2", 13 Sep. 2006, [Invited]
  • 三宅島2000年噴火堆積物中の鉄代謝微生物生態系               
    佐藤嘉則; 難波謙二; 加藤 拓; 東 照雄; 上條隆志; 太田寛行
    日本土壌肥料学会2006年度秋田大会, 07 Sep. 2006
  • 三宅島スコリア堆積物のガス代謝活性と細菌群集構造:植生レベルの異なる地点試料について               
    藤村玲子; 佐藤嘉則; 陸 洪省; 加藤広海; 片山葉子; 難波謙二; 加藤 拓; 東 照雄; 上條隆志; 太田寛行
    日本土壌肥料学会2006年度秋田大会, 06 Sep. 2006
  • 黒ボク土および褐色森林土の土壌断面における緑色成分の垂直分布               
    小林孝行; 渡邊眞紀子; 太田寛行; 藤嶽暢英
    日本土壌肥料学会2006年度秋田大会, 05 Sep. 2006
  • Microbial ecosystem development in the Miyake-jima (Japan) volcanic deposits ranging from 5 to >800 years old               
    R. Fujimura; Y. Sato; K. Nanba; H. Kato; T. Kato; T. Higashi; T. kamijyo; Y. katayama; H. Ohta
    11th International Symposium on Microbial Ecology, 22 Aug. 2006
  • Isolation and characterization of thallium-resistant bacteria from thallium-contaminated sediment and unpolluted soils               
    Z.H. Bao; M. Kubota; H. Ohta
    11th International Symposium on Microbial Ecology, 21 Aug. 2006
  • Enumeration of carbonyl sulfide-degrading heterotrophic microorganmisms in soil               
    H. Kato; R. Fujimura; Y. Sato; H. Ohta; Y. Katayama
    11th International Symposium on Microbial Ecology, 21 Aug. 2006
  • Lithotriohic bacterial community in the 2000-y volcanic ash deposit of the Miyake-jima (Japan)               
    Y. Sato; R. Fujimura; K. Nanba; T. Kato; T. Higashi; T. Kamijyo; Y. Katayama; H. Ohta
    11th International Symposium on Microbial Ecology, 21 Aug. 2006
  • Sphingobium amiense YT株におけるoctylphenol分解における共存細菌とcyclodextrinの効果               
    大塚美奈; 生長陽子; 長谷川守文; 久留主泰朗; 太田寛行
    日本土壌微生物学会2006年度仙台大会, 11 Jun. 2006
  • ノニルフェノールポリエトキシレート散布によるイネ葉圏細菌への影響               
    花田晶子; 吉田重信; 生長陽子; 小板橋基夫; 太田寛行; 對馬誠也
    日本土壌微生物学会2006年度仙台大会, 11 Jun. 2006
  • Microbes and global environmental change – greenhouse effect gas emission through microbial activities –               
    H. Ohta
    International Symposium on Academic Exchange Cooperation, Gadjah Mada Universitya and Ibaraki University-"Food and Environmental Preservation in Asian Agriculture", 24 Nov. 2005, [Invited]
  • Microbes and global environmental change – greenhouse effect gas emission through microbial activities –               
    H. Ohta
    International Symposium on Academic Exchange Cooperation, Udayana Universitya and Ibaraki University-"Food and Environmental Preservation in Asian Agriculture", 22 Nov. 2005, [Invited]
  • Shingobium amiense YT株によるオクチルフェノール分解:サイクロデキストリンの効果               
    大塚美奈; 生長陽子; 長谷川守文; 久留主泰朗; 太田寛行
    第21回日本微生物生態学会, 02 Nov. 2005
  • 三宅島2000年噴火堆積物中から分離された細菌の無機栄養代謝活性               
    藤村玲子; 佐藤嘉則; 片山葉子; 加藤広海; 加藤 拓; 上條隆志; 太田寛行
    第21回日本微生物生態学会, 01 Nov. 2005
  • 外生菌根菌Cenococcum geophilumの生化学的特徴               
    吉原亮治; 太田寛行; 藤嶽暢英; 渡邊眞紀子; 山田明義
    第21回日本微生物生態学会, 01 Nov. 2005
  • Shingobium amiense YT株によるアルキルフェノール代謝経路               
    生長陽子; 戸嶋浩明; 長谷川守文; 児玉 治; 太田寛行
    第21回日本微生物生態学会, 01 Nov. 2005
  • Shingobium amiense YT株におけるオクチルフェノールヒドロキシラーゼ酵素系の解析               
    末森光紘; 生長陽子; 長谷川守文; 太田寛行
    第21回日本微生物生態学会, 01 Nov. 2005
  • タリウム負荷土壌から分離された細菌の重金属耐性               
    包 智華; 久保田正亜; 太田寛行
    第21回日本微生物生態学会, 31 Oct. 2005
  • PA-46 Chemolithotrophic metabolism in bacteria isolated from the 2000-year erupution-volcanic deposits of Miyake-jima Island.(BIOGEOCHEMICAL CYCLES,Session A,(1) Poster presentations)               
    Fujimura,Reiko; Sato,Yoshinori; Katayama,Yoko; Kato,Hiromi; Kato,Taku; Kamijo,Takashi; Ohta,Hiroyuki
    日本微生物生態学会講演要旨集, Oct. 2005, Japanese Society of Microbial Ecology
    200510, 200510
  • Overview of greenhouse effect gas emission from agricultural soils through microbial activities               
    H. Ohta
    Ecological Analysis and Control of Greenhouse Gas Emission from Agriculture in Aisa, 15 Sep. 2005, [Invited]
  • 三宅島2000年噴火堆積物中の硫黄酸化細菌               
    佐藤嘉則; 加藤 拓; 上條隆志; 東 照雄; 片山葉子; 太田寛行
    日本土壌肥料学会2005年度島根大会, 06 Sep. 2005
  • 鉄型菌核粒子について               
    渡邊眞紀子; 上原英之; 井上 弦; 坂上伸生; 藤嶽暢英; 太田寛行
    日本土壌肥料学会2005年度島根大会, 06 Sep. 2005
  • 土壌菌核粒子の形成と遊離鉄               
    坂上伸生; 渡邊眞紀子; 太田寛行; 藤嶽暢英
    日本土壌肥料学会2005年度島根大会, 06 Sep. 2005
  • HPLC法による土壌中の緑色成分分離定量法の開発               
    小林孝行; 渡邊眞紀子; 太田寛行; 藤嶽暢英
    日本土壌肥料学会2005年度島根大会, 06 Sep. 2005
  • カバークロップが土壌微生物相に及ぼす影響について               
    昭日格図; 小松崎将一; 佐藤嘉則; 太田寛行; 笠原康裕
    2005年度日本本土壌微生物学会, 12 Jun. 2005
  • アーバスキュラー菌根菌Archaeospora leptotichaおよびその類縁菌のrDNA-ITS領域に基づく系統解析               
    西村伊吹; 澤木弘道; 篠崎直人; 吉田重信; 對馬誠也; 太田寛行; 斎藤雅典
    2005年度日本土壌微生物学会, 12 Jun. 2005
  • 初期土壌生成と微生物群集-火山噴出物中における物質代謝と微生物-               
    太田寛行; 佐藤嘉則
    日本ペドロジー学会2005年度大会, 03 Apr. 2005, [Invited]
  • 土壌菌核粒子の土壌腐植成分としての特徴               
    坂上伸生; 井上 弦; B. Oyuntsetseg; 平舘俊太郎; 渡邊眞紀子; 藤嶽暢英; 太田寛行
    日本ペドロジー学会2005年度大会, 03 Apr. 2005
  • カバークロップが土壌微生物相に及ぼす影響について(2005年度大会一般講演要旨)
    昭日 格図; 小松崎 将一; 佐藤 嘉則; 太田 寛行; 笠原 康裕
    土と微生物, 2005, 日本土壌微生物学会
    2005, 2005
  • 土壌におけるノニルフェノール系農薬の動態               
    高原義治; 太田寛行
    第20回日本微生物生態学会, 21 Nov. 2004
  • Rulianti, A. D., Y. Akama, and H. Ohta               
    Degradation of alkylphenol polyethoxylates by soil bacteria: a model study using soil percolation columns
    第20回日本微生物生態学会, 21 Nov. 2004
  • Sphingobium amiense YT株と共存細菌によるオクチルフェノール分解の解析               
    大塚美奈; 韓 宇波; 生長陽子; 久留主泰朗; 太田寛行
    第20回日本微生物生態学会, 21 Nov. 2004
  • 土壌からのdi(2-ethylhexyl)phthalate(DEHP)分解菌の分離               
    山内三和; 星 侑希; 佐藤嘉則; 高原義治; 太田寛行
    第20回日本微生物生態学会, 21 Nov. 2004
  • イネ葉面より分離されたSphingomonas属細菌におけるアルキルフェノール系化合物の分解性               
    生長陽子; 篠原弘亮; 對馬誠也; 太田寛行
    第20回日本微生物生態学会, 21 Nov. 2004
  • 三宅島火山灰堆積層における無機栄養細菌の分布               
    佐藤嘉則; 西原宏史; 加藤 拓; 東 照雄; 上條隆志; 太田寛行
    第20回日本微生物生態学会, 21 Nov. 2004
  • Development of bacterial community on fresh volcanic deposits               
    H. Ohta; Y. Sato
    The Second Okazaki Biology Conference on "Terra Microbiology", 28 Sep. 2004, [Invited]
  • Phylogenetic diversity of bacterial community in the Mt. Pinatubo pyroclastic deposit               
    Y. Sato; K. Ogiwara; H. Nishihara; M. Yoshida; M. Watanabe; and H. Ohta
    The 10th International Symposium on Microbial Ecology, 26 Aug. 2004
  • Degradation profiles of alkylphenols by two strains belong to the Sphingomonas group               
    Y. Ikunaga; M. Hasegawa; Y. Kasahara; H. Ohta
    The 10th International Symposium on Microbial Ecology, 26 Aug. 2004
  • Degradation of tert-octylphenol by Sphingobium amiense               
    S.-I. Miyakawa; M. Suemori; M. Hasegawa; O. Kodama; H. Ohta
    The 10th International Symposium on Microbial Ecology, 26 Aug. 2004
  • Regulation of leukotoxin production by the oral-periodonto-pathogen Actinobacillus actinomycetemcomitans               
    U. Ushiba; T. Inoue; R. Shingaki; K. Fukui and H. Ohta
    The 10th International Symposium on Microbial Ecology, 26 Aug. 2004
  • Bacterial diversity and sulfur cycle-driving bacteria in the early microbial ecosystem of scidic volcanic ash deposits from the 2000-year eruption of the Miyake-jima Island, Japan               
    H. Ohta; S. Kamimura; M. Sato-Hirako; Y. Sato; C. Bao; H. Lu; T. Kato; T. Kamijyo; T. Higashi
    The 10th International Symposium on Microbial Ecology, 24 Aug. 2004
  • タリウム耐性土壌細菌の分離と類別               
    包 智華; 佐藤嘉則; 久保田正亜; 太田寛行
    2004年度日本土壌微生物学会, 04 Jun. 2004
  • Actinobacillus actinomycetemcomitansにおける白血球毒素産生とカタボライト抑制の関係               
    牛場裕司; 井上哲圭; 新垣隆資; 福井一博; 太田寛行
    第77回日本細菌学会総会, 03 Apr. 2004
  • 土壌からHCl-DMSOで抽出される緑色色素について(2. 土壌有機・無機化学, 2004年度大会講演要旨集)
    小林 孝行; 浅川 大地; 柳 由貴子; 鈴木 武志; 渡邊 眞紀子; 太田 寛行; 藤嶽 暢英
    日本土壌肥料学会講演要旨集, 2004, 一般社団法人 日本土壌肥料学会
    2004, 2004
  • Status of Aluminum and Humus as the Regulating Factor of the Growth of Sclerotium Grain in Forest Soils, Japan.               
    SAKAGAMI N; WATANABE M; OHTA H; FUJITAKE Nobuhide
    Proceedings of the 6th International Symposium on Plant-Soil Interaction at Low pH PSILPH., 2004
    2004, 2004
  • タリウム負荷土壌懸濁液における細菌群集の動態解析               
    包 智華; 久保田正亜; 太田寛行
    日本微生物生態学会第19回大会, 29 Oct. 2003
  • Sphingomonas属細菌を宿主とするベクター系の開発               
    斎藤美有紀; 生長陽子; 太田寛行; 久留主泰朗
    日本微生物生態学会第19回大会, 27 Oct. 2003
  • Phenolic compounds-degrading Sphingomonas spp. in fungal sclerotium-like grains from Mt. Myko (Japan) volcanic ash soil               
    Ohta; H.; Yagi; M.; Suzuki; J.; Fujitake; N. and Watanabe; M.
    16th International Symposium on Environmental Biogeochemistry, 05 Sep. 2003
  • Physiological and biochemical comparison of two nonylphenol-degrading Sphingomonas strains               
    Ikunaga; Y.; Hasegawa; M.; Kasahara; Y. and Ohta; H.
    16th International Symposium on Environmental Biogeochemistry, 05 Sep. 2003
  • Morphology, chemical properties and distribution of sclerotium grains studied in foreast soils               
    Watanabe; M; Fujitake; N. and Ohta; H.
    16th International Symposium on Environmental Biogeochemistry, 03 Sep. 2003
  • 7 フィリピン・ピナツボ火山泥流物質から形成された周辺土壌中の腐植の特徴(関東支部講演会)               
    佐々木 玲奈; 吉田 正夫; 大津 豊; 宮平 美樹; 太田 寛行; 渡邊 眞紀子
    日本土壌肥料学会講演要旨集, 20 Aug. 2003, 一般社団法人日本土壌肥料学会
    20030820, 20030820
  • 2-33 土壌菌核粒子内にみられる生体鉱物について(2.土壌有機・無機化学)               
    渡邊 眞紀子; 坂上 伸生; 藤嶽 暢英; 太田 寛行; 井上 弦
    日本土壌肥料学会講演要旨集, 20 Aug. 2003, 一般社団法人日本土壌肥料学会
    20030820, 20030820
  • Sphingomonas amiensisとSphingomonas cloacaeによるノニルフェノール分解特性の解析               
    生長陽子; 長谷川守文; 笠原康裕; 太田寛行
    日本土壌微生物学会2003年度大会, 13 Jul. 2003
  • Sphingomonas amiensisによるアルキルフェノール分解に関する研究               
    宮川修一; 生長陽子; 長谷川守文; 太田寛行; 児玉 治
    日本農薬学会第28回大会, 24 Mar. 2003
  • 高濃度及び低濃度ノニルフェノール分解性Sphingomonas属細菌における分解特性の解析               
    生長陽子; 高原義治; 久留主泰朗; 長谷川守文; 白井 誠; 太田寛行
    日本微生物生態学会第18回大会, 16 Nov. 2002
  • 細菌混合培養系におけるノニルフェノール分解特性の解析               
    韓 宇波; 高原義治; 生長陽子; 太田寛行
    日本微生物生態学会第18回大会, 15 Nov. 2002
  • 火山噴出物堆積層中の微生物世界               
    太田寛行
    日本微生物生態学会第18回大会、シンポジウム「土壌における生態系形成と微生物」, 15 Nov. 2002, [Invited]
  • ノニルフェノール(NP)分解性Sphingomonas sp. YTと共存するNP非分解性細菌の分類学的研究               
    韓 宇波; 牛場裕司; 高原義治; 笠原康裕; 太田寛行
    日本微生物生態学会第18回大会, 15 Nov. 2002
  • フィリピン・ピナツボ火山泥流堆積物から分離したRalstonia sp.の分類学的研究               
    佐藤嘉則; 西原宏史; 吉田正夫; 渡邊真紀子; 太田寛行
    日本微生物生態学会第18回大会, 15 Nov. 2002
  • A study of groundwater resource in the Pinatubo lahar affected area, Pasig-Potrro river and Porac river basin, Philippines               
    Hiraide; S.; Morishima; W.; Watanabe; M.; Ohta; H.; Yoshida; M.; Collado; M. B.; Rondal; J. D.; Suzuki; K. and Kinugasa; Y.
    International Conference on Water Resources and Environment Research (ICMRER), 22 Jul. 2002
  • 森林土壌中の菌核様粒子から分離された細菌の特徴               
    鈴木順子; 佐藤嘉則; 藤嶽暢英; 渡邊真紀子; 太田寛行
    日本土壌微生物学会2002年度大会, 05 Jun. 2002
  • 三宅島火山灰堆積層中の微生物分析               
    太田寛行; 平子めぐみ; 佐藤嘉則; 加藤 拓; 上條隆志; 東 照雄
    日本土壌肥料学会2002年度名古屋大会, 04 Apr. 2002
  • 有機物分解限界を微生物の生理から考える               
    太田寛行
    日本土壌肥料学会2002年度名古屋大会、シンポジウム「土壌生物圏はどこまで有機物資源をリサイクルできるか?」, 02 Apr. 2002, [Invited]
  • 13-10 テフラに存在する腐植酸Pgの特性と成因について(13.土壌生成・分類)               
    青木 一恵; 渡邊 眞紀子; 平山 良治; 太田 寛行; 藤嶽 暢英
    日本土壌肥料学会講演要旨集, 25 Mar. 2002, 一般社団法人日本土壌肥料学会
    20020325, 20020325
  • 森林土壌中の菌核様粒子から分離された細菌の特徴(2002年度大会一般講演要旨)
    鈴木 順子; 佐藤 嘉則; 藤嶽 暢英; 渡邊 眞紀子; 太田 寛行
    土と微生物, 2002, 日本土壌微生物学会
    2002, 2002
  • Sphingomonas sp. YT株の低濃度ノニルフェノールの分解性               
    生長陽子; 久留主泰朗; 笠原康裕; 太田寛行
    日本微生物生態学会第17回大会, 11 Nov. 2001, 日本微生物生態学会
  • 微好気性細菌の増殖と環境中のカリウム:口腔細菌Actinobacillus actinomycetemcomitansの場合               
    太田寛行; 井上哲圭; 福井一博
    日本微生物生態学会第17回大会, 09 Nov. 2001, 日本微生物生態学会
  • 河川底質から分離されたノニルフェノール分解性Sphingomonas sp. YT株の分類学的性質               
    牛場裕司; 太田寛行
    日本微生物生態学会第17回大会, 09 Nov. 2001, 日本微生物生態学会
  • ピナツボ・ラハール地帯の窒素の集積と細菌フロラ               
    太田寛行; 荻原一也; 佐藤嘉則; 高橋寛至; 吉田正夫; 渡邊眞紀子
    日本土壌微生物学会2001年度大会, 24 May 2001, 日本土壌微生物学会
  • 6-24 ピナツボ・ラハール地帯パイオニア植物の根圏細菌フロラ(6.土壌生物)               
    佐藤 嘉則; 荻原 一也; 吉田 正夫; 渡邊 眞紀子; 太田 寛行
    日本土壌肥料学会講演要旨集, 25 Mar. 2001, 一般社団法人日本土壌肥料学会
    20010325, 20010325
  • ピナツボ・ラハール地帯の窒素の集積と細菌フロラ(2001年度大会一般講演要旨)
    太田 寛行; 荻原 一也; 佐藤 嘉則; 高橋 寛至; 吉田 正夫; 渡邊 眞紀子
    土と微生物, 2001, 日本土壌微生物学会
    2001, 2001
  • Degradation of branched nonylphenol by Sphingomonas sp. strain YT               
    de Vries; Y. P.; Y. Takahara; Y. Ikunaga; M. Hasegawa; H. Ohta
    日本微生物生態学会第16回大会, 14 Nov. 2000, 日本微生物生態学会
  • 河川底泥から分離したノニルフェノール分解菌群の解析               
    高原義治; 太田寛行
    日本土壌肥料学会2000年度東京大会, 04 Apr. 2000, 日本土壌肥料学会
  • フィリピン・ピナツボ火山泥流堆積物の非培養法による微生物群集構造解析               
    荻原一也; 村上恵理; 河村絢子; 吉田正夫; 渡邊眞紀子; 太田寛行
    日本土壌肥料学会2000年度東京大会, 03 Apr. 2000, 日本土壌肥料学会
  • 妙高燕土壌断面における菌核の分布と化学組成               
    加戸 卓; 渡邊眞紀子; 藤嶽暢英; 太田寛行
    日本土壌肥料学会2000年度東京大会, 02 Apr. 2000, 日本土壌肥料学会
  • 妙高燕土壌中の菌核粒子微生物フロラの解析               
    八木通江; 生長陽子; 藤嶽暢英; 太田寛行; 渡邊眞紀子
    日本土壌肥料学会2000年度東京大会, 02 Apr. 2000, 日本土壌肥料学会
  • 24-31 フィリピン、ピナツボ火山泥流物質中の窒素の年代経過に伴う蓄積挙動(24.地球環境)               
    高橋 寛至; 澄川 慎一朗; 吉田 正夫; 太田 寛行; 渡辺 真紀子
    日本土壌肥料学会講演要旨集, 25 Mar. 2000, 一般社団法人日本土壌肥料学会
    20000325, 20000325
  • 6-9 フィリピン・ピナツボ火山泥流堆積物の非培養法による微生物群集構造解析(6.土壌生物)               
    荻原 一也; 村上 恵理; 河村 絢子; 吉田 正夫; 渡辺 眞紀子; 太田 寛行
    日本土壌肥料学会講演要旨集, 25 Mar. 2000, 一般社団法人日本土壌肥料学会
    20000325, 20000325
  • 6-6 妙高燕土壌中の菌核粒子微生物フロラの解析(6.土壌生物)               
    八木 通江; 生長 陽子; 藤嶽 暢英; 太田 寛行; 渡辺 眞紀子
    日本土壌肥料学会講演要旨集, 25 Mar. 2000, 一般社団法人日本土壌肥料学会
    20000325, 20000325
  • 6-5 妙高燕土壌断面における菌核の分布と化学組成(6.土壌生物)               
    加戸 卓; 渡邊 眞紀子; 藤嶽 暢英; 太田 寛行
    日本土壌肥料学会講演要旨集, 25 Mar. 2000, 一般社団法人日本土壌肥料学会
    20000325, 20000325
  • フィリピン・ピナツボ火山泥流堆積物中の硫黄酸化細菌の動態               
    河村絢子; 太田寛行; 澄川慎一郎; 渡辺紀子; 吉田正夫; 渡邊眞紀子; 片山葉子
    日本微生物生態学会第15回大会, 07 Nov. 1999, 日本微生物生態学会
  • フィリピン・ピナツボ火山泥流堆積物における植生回復と土壌微生物               
    荻原一也; 関口 勝; 渡邊眞紀子; 吉田正夫
    日本土壌肥料学会1999年度北海道大会, 31 Jul. 1999, 日本土壌肥料学会
  • 6-5 フィリピン・ピナツボ火山泥流堆積物における植生回復と土壌微生物(6.土壌生物)               
    荻原 一也; 関口 勝; 渡辺 真紀子; 吉田 正夫; 太田 寛行
    日本土壌肥料学会講演要旨集, 25 Jul. 1999, 一般社団法人日本土壌肥料学会
    19990725, 19990725
  • フィリピン・ピナツボ火山新生火山灰中の微生物               
    太田寛行; 荻原一也; 関口 勝; 越田淳一; 染谷 孝; 渡邊眞紀子; 吉田正夫
    日本微生物生態学会第14回大会, 21 Nov. 1998, 日本微生物生態学会
  • 土壌低栄養性細菌のフェノール性酸利用性               
    太田寛行; 久保田正亜
    日本土壌肥料学会1998年度大阪大会, 04 Apr. 1998, 日本土壌肥料学会

Affiliated academic society

  • 2009, International Society for Environmental Biogeochemistry
  • Jan. 1985, Japanese Society for Soil Microbiology
  • 日本土壌肥料学会
  • 日本農芸化学会
  • The Society for General Microbiology
  • American Society for Microbiology
  • International Society of Microbial Ecology
  • 日本土壌微生物学会

Research Themes