A distinct double-ring LH1–LH2 photocomplex from an extremophilic phototroph
Kazutoshi Tani; Kenji V. P. Nagashima; Risa Kojima; Masaharu Kondo; Ryo Kanno; Issei Satoh; Mai Kawakami; Naho Hiwatashi; Kazuna Nakata; Sakiko Nagashima; Kazuhito Inoue; Yugo Isawa; Ryoga Morishita; Shinichi Takaichi; Endang R. Purba; Malgorzata Hall; Long-Jiang Yu; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Yukihiro Kimura; Yutaka Nagasawa; Takehisa Dewa; Zheng-Yu Wang-Otomo, Last, Springer Science and Business Media LLC
Nature Communications, 06 Feb. 2025, [Reviewed]

Genomic highlights of the phylogenetically unique halophilic purple nonsulfur bacterium, Rhodothalassium salexigens
Michael T. Madigan; Kelly S. Bender; Mary N. Parenteau; Yukihiro Kimura; Zheng-Yu Wang-Otomo; W. Matthew Sattley, Abstract

Rhodothalassium (Rts.) salexigens is a halophilic purple nonsulfur bacterium and the sole species in the genus Rhodothalassium, which is itself the sole genus in the family Rhodothalassiaceae and sole family in the order Rhodothalassiales (class Alphaproteobacteria). The genome of this phylogenetically unique phototroph comprises 3.35 Mb and is highly chimeric, with nearly half of its genes originating from families other than the Rhodothalassiaceae, many of which lack phototrophic species. Photosynthesis genes in Rts. salexigens are not arranged in a typical photosynthesis gene cluster but are scattered across the genome, suggesting an origin from horizontal transfers. Despite an encoded RuBisCO, autotrophy has not been observed in Rts. salexigens, and enzymes that oxidize common inorganic electron donors are not encoded. Phospholipid biosynthesis in Rts. salexigens is restricted, and phosphoglycerolipids are the only phospholipids present in its intracytoplasmic membranes. Rts. salexigens fixes nitrogen using a Mo-containing nitrogenase and uses ammonia despite previous results that indicated it was a glutamate auxotroph. Glycine betaine is the sole osmolyte in Rts. salexigens, and enzymes are encoded that facilitate both its uptake and its biosynthesis from glycine. The genomic data also support chemotactic swimming motility, growth over a range of salinities, and the production of membrane-strengthening hopanoids., Springer Science and Business Media LLC
Extremophiles, 25 Jan. 2025, [Reviewed]

A Native LH1–RC–HiPIP Supercomplex from an Extremophilic Phototroph
Kazutoshi Tani; Ryo Kanno; Kenji V. P. Nagashima; Mai Kawakami; Naho Hiwatashi; Kazuna Nakata; Sakiko Nagashima; Kazuhito Inoue; Shinichi Takaichi; Endang R. Purba; Malgorzata Hall; Long-Jiang Yu; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Yukihiro Kimura; Zheng-Yu Wang-Otomo, Last, Springer Science and Business Media LLC
Communications Biology, 11 Jan. 2025, [Reviewed]

Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum
Xiang-Ping Wang; Guang-Lei Wang; Yuan Fu; Akane Minamino; Mei-Juan Zou; Fei Ma; Bo Xu; Zheng-Yu Wang-Otomo; Yukihiro Kimura; Michael T. Madigan; Jörg Overmann; Long-Jiang Yu, Springer Science and Business Media LLC
Communications Biology, 19 Dec. 2024, [Reviewed]

The Thermal-Stable LH1–RC Complex of a Hot Spring Purple Bacterium Powers Photosynthesis with Extremely Low-Energy Near-Infrared Light
Yukihiro Kimura; Ryo Kanno; Kaisei Mori; Yoshiki Matsuda; Ryuta Seto; Shinji Takenaka; Hiroyuki Mino; Tatsunari Ohkubo; Mai Honda; Yuji C. Sasaki; Jun-ichi Kishikawa; Kaoru Mitsuoka; Kazuhiro Mio; Malgorzata Hall; Endang R. Purba; Toshiaki Mochizuki; Akira Mizoguchi; Bruno M. Humbel; Michael T. Madigan; Zheng-Yu Wang-Otomo; Kazutoshi Tani, American Chemical Society (ACS)
Biochemistry, 16 Dec. 2024, [Reviewed]

Spectral modulation of B850 bacteriochlorophyll a in light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum by detergents and calcium ions
Yoshitaka Saga; Yuhi Sasamoto; Kazuki Inada; Zheng-Yu Wang-Otomo; Yukihiro Kimura, Elsevier BV
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Aug. 2024, [Reviewed]

High-resolution structure and biochemical properties of the LH1–RC photocomplex from the model purple sulfur bacterium, Allochromatium vinosum
Kazutoshi Tani; Ryo Kanno; Ayaka Harada; Yuki Kobayashi; Akane Minamino; Shinji Takenaka; Natsuki Nakamura; Xuan-Cheng Ji; Endang R. Purba; Malgorzata Hall; Long-Jiang Yu; Michael T. Madigan; Akira Mizoguchi; Kenji Iwasaki; Bruno M. Humbel; Yukihiro Kimura; Zheng-Yu Wang-Otomo, Last, Abstract

The mesophilic purple sulfur phototrophic bacterium Allochromatium (Alc.) vinosum (bacterial family Chromatiaceae) has been a favored model for studies of bacterial photosynthesis and sulfur metabolism, and its core light-harvesting (LH1) complex has been a focus of numerous studies of photosynthetic light reactions. However, despite intense efforts, no high-resolution structure and thorough biochemical analysis of the Alc. vinosum LH1 complex have been reported. Here we present cryo-EM structures of the Alc. vinosum LH1 complex associated with reaction center (RC) at 2.24 Å resolution. The overall structure of the Alc. vinosum LH1 resembles that of its moderately thermophilic relative Alc. tepidum in that it contains multiple pigment-binding α- and β-polypeptides. Unexpectedly, however, six Ca ions were identified in the Alc. vinosum LH1 bound to certain α1/β1- or α1/β3-polypeptides through a different Ca²⁺-binding motif from that seen in Alc. tepidum and other Chromatiaceae that contain Ca²⁺-bound LH1 complexes. Two water molecules were identified as additional Ca²⁺-coordinating ligands. Based on these results, we reexamined biochemical and spectroscopic properties of the Alc. vinosum LH1–RC. While modest but distinct effects of Ca²⁺ were detected in the absorption spectrum of the Alc. vinosum LH1 complex, a marked decrease in thermostability of its LH1–RC complex was observed upon removal of Ca²⁺. The presence of Ca²⁺ in the photocomplex of Alc. vinosum suggests that Ca²⁺-binding to LH1 complexes may be a common adaptation in species of Chromatiaceae for conferring spectral and thermal flexibility on this key component of their photosynthetic machinery., Springer Science and Business Media LLC
Communications Biology, 12 Feb. 2024, [Reviewed]

Genomic basis for the unique phenotype of the alkaliphilic purple nonsulfur bacterium Rhodobaca bogoriensis
Michael T. Madigan; Kelly S. Bender; Sophia A. Sanguedolce; Mary N. Parenteau; Marisa H. Mayer; Yukihiro Kimura; Zheng-Yu Wang-Otomo; W. Matthew Sattley
EXTREMOPHILES, Aug. 2023, [Reviewed]

Atomic force microscopic analysis of the light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum.
Masayuki Morimoto; Haruna Hirao; Masaharu Kondo; Takehisa Dewa; Yukihiro Kimura; Zheng-Yu Wang-Otomo; Hitoshi Asakawa; Yoshitaka Saga, Structural information on the circular arrangements of repeating pigment-polypeptide subunits in antenna proteins of purple photosynthetic bacteria is a clue to a better understanding of molecular mechanisms for the ring-structure formation and efficient light harvesting of such antennas. Here, we have analyzed the ring structure of light-harvesting complex 2 (LH2) from the thermophilic purple bacterium Thermochromatium tepidum (tepidum-LH2) by atomic force microscopy. The circular arrangement of the tepidum-LH2 subunits was successfully visualized in a lipid bilayer. The average top-to-top distance of the ring structure, which is correlated with the ring size, was 4.8 ± 0.3 nm. This value was close to the top-to-top distance of the octameric LH2 from Phaeospirillum molischianum (molischianum-LH2) by the previous analysis. Gaussian distribution of the angles of the segments consisting of neighboring subunits in the ring structures of tepidum-LH2 yielded a median of 44°, which corresponds to the angle for the octameric circular arrangement (45°). These results indicate that tepidum-LH2 has a ring structure consisting of eight repeating subunits. The coincidence of an octameric ring structure of tepidum-LH2 with that of molischianum-LH2 is consistent with the homology of amino acid sequences of the polypeptides between tepidum-LH2 and molischianum-LH2.
Photosynthesis research, 17 Mar. 2023

Rhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex.
Kazutoshi Tani; Ryo Kanno; Xuan-Cheng Ji; Itsusei Satoh; Yuki Kobayashi; Malgorzata Hall; Long-Jiang Yu; Yukihiro Kimura; Akira Mizoguchi; Bruno M Humbel; Michael T Madigan; Zheng-Yu Wang-Otomo, Rhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the light-harvesting 1-reaction center (LH1-RC) complex, but lacks protein-U, a U-shaped polypeptide in the LH1-RC of its close relative Rba. sphaeroides. Here we present a cryo-EM structure of the Rba. capsulatus LH1-RC purified by DEAE chromatography. The crescent-shaped LH1-RC exhibits a compact structure containing only 10 LH1 αβ-subunits. Four αβ-subunits corresponding to those adjacent to protein-U in Rba. sphaeroides were absent. PufX in Rba. capsulatus exhibits a unique conformation in its N-terminus that self-associates with amino acids in its own transmembrane domain and interacts with nearby polypeptides, preventing it from interacting with proteins in other complexes and forming dimeric structures. These features are discussed in relation to the minimal requirements for the formation of LH1-RC monomers and dimers, the spectroscopic behavior of both the LH1 and RC, and the bioenergetics of energy transfer from LH1 to the RC.
Nature communications, 15 Feb. 2023

Advances in the Spectroscopic and Structural Characterization of Core Light-Harvesting Complexes from Purple Phototrophic Bacteria
Yukihiro Kimura; Kazutoshi Tani; Michael T. Madigan; Zheng-Yu Wang-Otomo, American Chemical Society (ACS)
The Journal of Physical Chemistry B, 03 Jan. 2023

Selective Expression of Light-Harvesting Complexes Alters Phospholipid Composition in the Intracytoplasmic Membrane and Core Complex of Purple Phototrophic Bacteria
I. Satoh; K. Gotou; S. Nagatsuma; K. V; P. Nagashima; M. Kobayashi; L.-J. Yu; M. T. Madigan; Y. Kimura; Zheng-Yu Wang-Otomo, Elsevier BV
Biochim. Biophy. Acta, 2023, [Reviewed]

Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1–RC complex
Kazutoshi Tani; Ryo Kanno; Riku Kikuchi; Saki Kawamura; Kenji V. P. Nagashima; Malgorzata Hall; Ai Takahashi; Long-Jiang Yu; Yukihiro Kimura; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Zheng-Yu Wang-Otomo, Abstract

Rhodobacter sphaeroides is a model organism in bacterial photosynthesis, and its light-harvesting-reaction center (LH1–RC) complex contains both dimeric and monomeric forms. Here we present cryo-EM structures of the native LH1–RC dimer and an LH1–RC monomer lacking protein-U (ΔU). The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. PufX plays a critical role in connecting the two monomers in a dimer, with one PufX interacting at its N-terminus with another PufX and an LH1 β-polypeptide in the other monomer. One protein-U was only partially resolved in the dimeric structure, signaling different degrees of disorder in the two monomers. The ΔU LH1–RC monomer was half-moon-shaped and contained 11 α- and 10 β-polypeptides, indicating a critical role for protein-U in controlling the number of αβ-subunits required for dimer assembly and stabilization. These features are discussed in relation to membrane topology and an assembly model proposed for the native dimeric complex., Springer Science and Business Media LLC
Nature Communications, Dec. 2022

An LH1–RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins
Kazutoshi Tani; Ryo Kanno; Keigo Kurosawa; Shinichi Takaichi; Kenji V. P. Nagashima; Malgorzata Hall; Long-Jiang Yu; Yukihiro Kimura; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Zheng-Yu Wang-Otomo, Abstract

Rhodopila globiformis is the most acidophilic of anaerobic purple phototrophs, growing optimally in culture at pH 5. Here we present a cryo-EM structure of the light-harvesting 1–reaction center (LH1–RC) complex from Rhodopila globiformis at 2.24 Å resolution. All purple bacterial cytochrome (Cyt, encoded by the gene pufC) subunit-associated RCs with known structures have their N-termini truncated. By contrast, the Rhodopila globiformis RC contains a full-length tetra-heme Cyt with its N-terminus embedded in the membrane forming an α-helix as the membrane anchor. Comparison of the N-terminal regions of the Cyt with PufX polypeptides widely distributed in Rhodobacter species reveals significant structural similarities, supporting a longstanding hypothesis that PufX is phylogenetically related to the N-terminus of the RC-bound Cyt subunit and that a common ancestor of phototrophic Proteobacteria contained a full-length tetra-heme Cyt subunit that evolved independently through partial deletions of its pufC gene. Eleven copies of a novel γ-like polypeptide were also identified in the bacteriochlorophyll a-containing Rhodopila globiformis LH1 complex; γ-polypeptides have previously been found only in the LH1 of bacteriochlorophyll b-containing species. These features are discussed in relation to their predicted functions of stabilizing the LH1 structure and regulating quinone transport under the warm acidic conditions., Springer Science and Business Media LLC
Communications Biology, 07 Nov. 2022, [Reviewed]

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species
Yukihiro Kimura; Kazuna Nakata; Shingo Nojima; Shinji Takenaka; Michael T. Madigan; Zheng-Yu Wang-Otomo, Halorhodospira (Hlr.) species are the most halophilic and alkaliphilic of all purple bacteria. Hlr. halochloris exhibits the lowest LH1 Qy transition energy among phototrophic organisms and is the only known triply extremophilic anoxygenic phototroph, displaying a thermophilic, halophilic, and alkaliphilic phenotype. Recently, we reported that electrostatic charges are responsible for the unusual spectroscopic properties of the Hlr. halochloris LH1 complex. In the present work, we examined the effects of salt and pH on the spectroscopic properties and thermal stability of LH1-RCs from Hlr. halochloris compared with its mesophilic counterpart, Hlr. abdelmalekii. Experiments in which the photocomplexes were subjected to different levels of salt or variable pH revealed that the thermal stability of LH1-RCs from both species was largely retained in the presence of high salt concentrations and/or at alkaline pH but was markedly reduced by lowering the salt concentration and/or pH. Based on the amino acid sequences of LH1 polypeptides and their composition of acidic/basic residues and the Hofmeister series for cation/anion species, we discuss the importance of electrostatic charge in stabilizing the Hlr. halochloris LH1-RC complex to allow it to perform photosynthesis in its warm, hypersaline, and alkaline habitat., MDPI AG
Microorganisms, 02 May 2022

A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes
Kazutoshi Tani; Kazumi Kobayashi; Naoki Hosogi; Xuan-Cheng Ji; Sakiko Nagashima; Kenji V.P. Nagashima; Airi Izumida; Kazuhito Inoue; Yusuke Tsukatani; Ryo Kanno; Malgorzata Hall; Long-Jiang Yu; Isamu Ishikawa; Yoshihiro Okura; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Yukihiro Kimura; Zheng-Yu Wang-Otomo, Elsevier BV
Journal of Biological Chemistry, Apr. 2022

Correction to: Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria.
Michael T Madigan; Jill N Absher; Joseph E Mayers; Marie Asao; Deborah O Jung; Kelly S Bender; Megan L Kempher; Mackenzie K Hayward; Sophia A Sanguedolce; Abigail C Brown; Shinichi Takaichi; Ken Kurokawa; Atsushi Toyoda; Hiroshi Mori; Yusuke Tsukatani; Zheng-Yu Wang-Otomo; David M Ward; W Matthew Sattley
Archives of microbiology, 11 Mar. 2022

Identification of metal-sensitive structural changes in the Ca2+-binding photocomplex from Thermochromatium tepidum by isotope-edited vibrational spectroscopy
Yukihiro Kimura; Michie Imanishi; Li Yong; Yuki Yura; Takashi Ohno; Yoshitaka Saga; Michael T Madigan; Zheng-Yu Wang-Otomo, AIP Publishing
The Journal of Chemical Physics, 14 Feb. 2022

Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria.
Michael T Madigan; Jill N Absher; Joseph E Mayers; Marie Asao; Deborah O Jung; Kelly S Bender; Megan L Kempher; Mackenzie K Hayward; Sophia A Sanguedolce; Abigail C Brown; Shinichi Takaichi; Ken Kurokawa; Atsushi Toyoda; Hiroshi Mori; Yusuke Tsukatani; Zheng-Yu Wang-Otomo; David M Ward; W Matthew Sattley, We describe a new species of purple sulfur bacteria (Chromatiaceae, anoxygenic phototrophic bacteria) isolated from a microbial mat in the sulfidic geothermal outflow of a hot spring in Rotorua, New Zealand. This phototroph, designated as strain NZ, grew optimally near 45 °C but did not show an absorption maximum at 915 nm for the light-harvesting-reaction center core complex (LH1-RC) characteristic of other thermophilic purple sulfur bacteria. Strain NZ had a similar carotenoid composition as Thermochromatium tepidum, but unlike Tch. tepidum, grew photoheterotrophically on acetate in the absence of sulfide and metabolized thiosulfate. The genome of strain NZ was significantly larger than that of Tch. tepidum but slightly smaller than that of Allochromatium vinosum. Strain NZ was phylogenetically more closely related to mesophilic purple sulfur bacteria of the genus Allochromatium than to Tch. tepidum. This conclusion was reached from phylogenetic analyses of strain NZ genes encoding 16S rRNA and the photosynthetic functional gene pufM, from phylogenetic analyses of entire genomes, and from a phylogenetic tree constructed from the concatenated sequence of 1090 orthologous proteins. Moreover, average nucleotide identities and digital DNA:DNA hybridizations of the strain NZ genome against those of related species of Chromatiaceae supported the phylogenetic analyses. From this collection of properties, we describe strain NZ here as the first thermophilic species of the genus Allochromatium, Allochromatium tepidum NZT, sp. nov.
Archives of microbiology, 04 Jan. 2022

A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex
Kazutoshi Tani; Kenji V. P. Nagashima; Ryo Kanno; Saki Kawamura; Riku Kikuchi; Malgorzata Hall; Long-Jiang Yu; Yukihiro Kimura; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Zheng-Yu Wang-Otomo, Rhodobacter (Rba.) sphaeroides is the most widely used model organism in bacterial photosynthesis. The light-harvesting-reaction center (LH1-RC) core complex of this purple phototroph is characterized by the co-existence of monomeric and dimeric forms, the presence of the protein PufX, and approximately two carotenoids per LH1 αβ-polypeptides. Despite many efforts, structures of the Rba. sphaeroides LH1-RC have not been obtained at high resolutions. Here we report a cryo-EM structure of the monomeric LH1-RC from Rba. sphaeroides strain IL106 at 2.9 Å resolution. The LH1 complex forms a C-shaped structure composed of 14 αβ-polypeptides around the RC with a large ring opening. From the cryo-EM density map, a previously unrecognized integral membrane protein, referred to as protein-U, was identified. Protein-U has a U-shaped conformation near the LH1-ring opening and was annotated as a hypothetical protein in the Rba. sphaeroides genome. Deletion of protein-U resulted in a mutant strain that expressed a much-reduced amount of the dimeric LH1-RC, indicating an important role for protein-U in dimerization of the LH1-RC complex. PufX was located opposite protein-U on the LH1-ring opening, and both its position and conformation differed from that of previous reports of dimeric LH1-RC structures obtained at low-resolution. Twenty-six molecules of the carotenoid spheroidene arranged in two distinct configurations were resolved in the Rba. sphaeroides LH1 and were positioned within the complex to block its channels. Our findings offer an exciting new view of the core photocomplex of Rba. sphaeroides and the connections between structure and function in bacterial photocomplexes in general., Springer Science and Business Media LLC
Nature Communications, Dec. 2021

Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris
Yukihiro Kimura; Shingo Nojima; Kazuna Nakata; Takuya Yamashita; Xiang-Ping Wang; Shinji Takenaka; Seiji Akimoto; Masayuki Kobayashi; Michael T. Madigan; Zheng-Yu Wang-Otomo; Long-Jiang Yu
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Nov. 2021

Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Complex
Kenji V. P. Nagashima; Sakiko Nagashima; Masaharu Kitashima; Kazuhito Inoue; Michael T. Madigan; Yukihiro Kimura; Zheng-Yu Wang-Otomo
BIOCHEMISTRY, Sep. 2021

Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum
Kazutoshi Tani; Ryo Kanno; Xuan-Cheng Ji; Malgorzata Hall; Long-Jiang Yu; Yukihiro Kimura; Michael T. Madigan; Akira Mizoguchi; Bruno M. Humbel; Zheng-Yu Wang-Otomo
BIOCHEMISTRY, Aug. 2021

Exciton Origin of Color-Tuning in Ca2+-Binding Photosynthetic Bacteria
Kou Timpmann; Margus Ratsep; Liina Kangur; Alexandra Lehtmets; Zheng-Yu Wang-Otomo; Arvi Freiberg
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Jul. 2021

Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP
Tomoaki Kawakami; Long-Jiang Yu; Tai Liang; Koudai Okazaki; Michael T. Madigan; Yukihiro Kimura; Zheng-Yu Wang-Otomo
NATURE COMMUNICATIONS, Feb. 2021

Quinone transport in the closed light-harvesting 1 reaction center complex from the thermophilic purple bacterium Thermochromatium tepidum
Rikako Kishi; Michie Imanishi; Masayuki Kobayashi; Shinji Takenaka; Michael T. Madigan; Zheng-Yu Wang-Otomo; Yukihiro Kimura
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jan. 2021

Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides
Kazutoshi Tani; Ryo Kanno; Yuki Makino; Malgorzata Hall; Mizuki Takenouchi; Michie Imanishi; Long-Jiang Yu; Joerg Overmann; Michael T. Madigan; Yukihiro Kimura; Akira Mizoguchi; Bruno M. Humbel; Zheng-Yu Wang-Otomo
NATURE COMMUNICATIONS, Oct. 2020

The two light-harvesting membrane chromoproteins of Thermochromatium tepidum expose distinct robustness against temperature and pressure
Liina Kangur; Margus Ratsep; Kou Timpmann; Zheng-Yu Wang-Otomo; Arvi Freiberg
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Aug. 2020

Lycopene-Family Carotenoids Confer Thermostability on Photocomplexes from a New Thermophilic Purple Bacterium
Ryuta Seto; Shinichi Takaichi; Toshiyuki Kurihara; Rikako Kishi; Mai Honda; Shinji Takenaka; Yusuke Tsukatani; Michael T. Madigan; Zheng-Yu Wang-Otomo; Yukihiro Kimura
BIOCHEMISTRY, Jun. 2020

Direct injection of pigment–protein complexes and membrane fragments suspended in water from phototrophs to C18 HPLC
Shinichi Takaichi; Akira Okoshi; Seiu Otomo; Masahiro Misumi; Kintake Sonoike; Jiro Harada, Springer Science and Business Media {LLC}
Photosynthesis Research, 20 Apr. 2020, [Reviewed]

Blastochloris tepida, sp. nov., a thermophilic species of the bacteriochlorophyll b-containing genus Blastochloris
Michael T. Madigan; Sol M. Resnick; Megan L. Kempher; Alice C. Dohnalkova; Shinichi Takaichi; Zheng-Yu Wang-Otomo; Atsushi Toyoda; Ken Kurokawa; Hiroshi Mori; Yusuke Tsukatani
ARCHIVES OF MICROBIOLOGY, Dec. 2019

A Dual Role for Ca2+ in Expanding the Spectral Diversity and Stability of Light-Harvesting 1 Reaction Center Photocomplexes of Purple Phototrophic Bacteria
Michie Imanishi; Mizuki Takenouchi; Shinichi Takaich; Shiori Nakagawa; Yoshitaka Saga; Shinji Takenaka; Michael T. Madigan; Joerg Overmann; Zheng-Yu Wang-Otomo; Yukihiro Kimura
BIOCHEMISTRY, Jun. 2019

Phospholipid distributions in purple phototrophic bacteria and LH1-RC core complexes.
Nagatsuma S; Gotou K; Yamashita T; Yu LJ; Shen JR; Madigan MT; Kimura Y; Wang-Otomo ZY
Biochimica et biophysica acta. Bioenergetics, Jun. 2019, [Reviewed]

Novel features of LH1-RC from Thermochromatium tepidum revealed from its atomic resolution structure
Yu Long-Jiang; Suga Michihiro; Wang-Otomo Zheng-Yu; Shen Jian-Ren
FEBS JOURNAL, Dec. 2018, [Reviewed]

Cooperative Photoprotection by Multicompositional Carotenoids in the LH1 Antenna from a Mutant Strain of Rhodobacter sphaeroides
Yu Jie; Tan Li-Ming; Kawakami Tomoaki; Wang Peng; Fu Li-Min; Wang-Otomo Zheng-Yu; Zhang Jian-Ping
JOURNAL OF PHYSICAL CHEMISTRY B, 23 Aug. 2018, [Reviewed]

Biochemical and Spectroscopic Characterizations of a Hybrid Light-Harvesting Reaction Center Core Complex
Kimura Yukihiro; Hashimoto Kanako; Akimoto Seiji; Takenouchi Mizuki; Suzuki Kengo; Kishi Rikako; Imanishi Michie; Takenaka Shinji; Madigan Michael T; Nagashima Kenji V. P; Wang-Otomo Zheng-Yu
BIOCHEMISTRY, 31 Jul. 2018, [Reviewed]

New Insights into the Mechanism of Uphill Excitation Energy Transfer from Core Antenna to Reaction Center in Purple Photosynthetic Bacteria
Li-Ming Tan; Jie Yu; Tomoaki Kawakami; Masayuki Kobayashi; Peng Wang; Zheng-Yu Wang-Otomo; Jian-Ping Zhang
Journal of Physical Chemistry Letters, 21 Jun. 2018, [Reviewed]

Properties and structure of a low-potential, penta-heme cytochrome c552 from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum
Jing-Hua Chen; Long-Jiang Yu; Alain Boussac; Zheng-Yu Wang-Otomo; Tingyun Kuang; Jian-Ren Shen
Photosynthesis Research, 24 Apr. 2018, [Reviewed]

Structure of photosynthetic LH1-RC supercomplex at 1.9 Å resolution
Long-Jiang Yu; Michihiro Suga; Zheng-Yu Wang-Otomo; Jian-Ren Shen
Nature, 01 Apr. 2018, [Reviewed]

C-terminal cleavage of the LH1 α-polypeptide in the Sr2+-cultured Thermochromatium tepidum
Yukihiro Kimura; Tomoaki Kawakami; Teruhisa Arikawa; Yong Li; Long-Jiang Yu; Takashi Ohno; Michael T. Madigan; Zheng-Yu Wang-Otomo
Photosynthesis Research, 01 Mar. 2018, [Reviewed]

Carotenoid Singlet Fission Reactions in Bacterial Light Harvesting Complexes As Revealed by Triplet Excitation Profiles
Jie Yu; Li-Min Fu; Long-Jiang Yu; Ying Shi; Peng Wang; Zheng-Yu Wang-Otomo; Jian-Ping Zhang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Nov. 2017, [Reviewed]

Spectrally Selective Spectroscopy of Native Ca-Containing and Ba-Substituted LH1-RC Core Complexes from Thermochromatium tepidum
Margus Ratsep; Kou Timpmann; Tomoaki Kawakami; Zheng-Yu Wang-Otomo; Arvi Freiberg
JOURNAL OF PHYSICAL CHEMISTRY B, Nov. 2017, [Reviewed]

Probing structure-function relationships in early events in photosynthesis using a chimeric photocomplex
Kenji V. P. Nagashima; Mai Sasaki; Kanako Hashimoto; Shinichi Takaichi; Sakiko Nagashima; Long-Jiang Yu; Yuto Abe; Kenta Gotou; Tomoaki Kawakami; Mizuki Takenouchi; Yuuta Shibuya; Akira Yamaguchi; Takashi Ohno; Jian-Ren Shen; Kazuhito Inoue; Michael T. Madigan; Yukihiro Kimura; Zheng-Yu Wang-Otomo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Oct. 2017, [Reviewed]

Metal Cations Induced alpha beta-BChl a Heterogeneity in LH1 as Revealed by Temperature-Dependent Fluorescence Splitting
Fei Ma; Long-Jiang Yu; Manuel J. Llansola-Portoles; Bruno Robert; Zheng-Yu Wang-Otomo; Rienk van Grondelle
CHEMPHYSCHEM, Aug. 2017, [Reviewed]

Excitonic and Vibrational Coherence in the Excitation Relaxation Process of Two LH1 Complexes as Revealed by Two-Dimensional Electronic Spectroscopy
Fei Ma; Long-Jiang Yu; Ruud Hendrikx; Zheng-Yu Wang-Otomo; Rienk van Grondelle
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, Jun. 2017, [Reviewed]

Dependence of the hydration status of bacterial light-harvesting complex 2 on polyol cosolvents
Ying Shi; Jie Yu; Long-Jiang Yu; Peng Wang; Li-Min Fu; Jian-Ping Zhang; Zheng-Yu Wang-Otomo
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, May 2017, [Reviewed]

Effects of Calcium Ions on the Thermostability and Spectroscopic Properties of the LH1-RC Complex from a New Thermophilic Purple Bacterium Allochromatium tepidum
Yukihiro Kimura; Shuwen Lyu; Akira Okoshi; Koudai Okazaki; Natsuki Nakamura; Akira Ohashi; Takashi Ohno; Manami Kobayashi; Michie Imanishi; Shinichi Takaichi; Michael T. Madigan; Zheng-Yu Wang-Otomo
JOURNAL OF PHYSICAL CHEMISTRY B, May 2017, [Reviewed]

Direct Observation of Energy Detrapping in LH1-RC Complex by Two-Dimensional Electronic Spectroscopy
Fei Ma; Long-Jiang Yu; Ruud Hendrikx; Zheng-Yu Wang-Otomo; Rienk van Grondelle
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Jan. 2017, [Reviewed]

Spectroscopic and Thermodynamic Characterization of the Metal-Binding Sites in the LH1-RC Complex from Thermophilic Photosynthetic Bacterium Thermochromatium tepidum
Yukihiro Kimura; Yuki Yura; Yusuke Hayashi; Yong Li; Moe Onoda; Long-Jiang Yu; Zheng-Yu Wang-Otomo; Takashi Ohno
JOURNAL OF PHYSICAL CHEMISTRY B, Dec. 2016, [Reviewed]

Structural Basis for the Unusual Q(y) Red-Shift and Enhanced Thermostability of the LH1 Complex from Thermochromatium tepidum
Long-Jiang Yu; Tomoaki Kawakami; Yukihiro Kimura; Zheng-Yu Wang-Otomo
BIOCHEMISTRY, Nov. 2016, [Reviewed]

Challenges facing an understanding of the nature of low-energy excited states in photosynthesis
Jeffrey R. Reimers; Malgorzata Biczysko; Douglas Bruce; David F. Coker; Terry J. Frankcombe; Hideki Hashimoto; Juergen Hauer; Ryszard Jankowiak; Tobias Kramer; Juha Linnanto; Fikret Mamedov; Frank Mueh; Margus Ratsep; Thomas Renger; Stenbjom Styring; Jian Wan; Zhuan Wang; Zheng-Yu Wang-Otomo; Yu-Xiang Weng; Chunhong Yang; Jian-Ping Zhang; Arvi Freiberg; Elmars Krausz
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Sep. 2016, [Reviewed]

Bacterial Light-Harvesting Complexes Showing Giant Second-Order Nonlinear Optical Response as Revealed by Hyper-Rayleigh Light Scattering
Fei Ma; Long-Jiang Yu; Xiao-Hua Ma; Peng Wang; Zheng-Yu Wang-Otomo; Jian-Ping Zhang
JOURNAL OF PHYSICAL CHEMISTRY B, Sep. 2016, [Reviewed]

Temperature dependent LH1 -> RC energy transfer in purple bacteria Tch. tepidum with shiftable LH1-Q(y) band: A natural system to investigate thermally activated energy transfer in photosynthesis
Fei Ma; Long-Jiang Yu; Zheng-Yu Wang-Otomo; Rienk van Grondelle
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Apr. 2016, [Reviewed]

The origin of the unusual Q(y) red shift in LH1-RC complexes from purple bacteria Thermochromatium tepidum as revealed by Stark absorption spectroscopy
Fei Ma; Long-Jiang Yu; Zheng-Yu Wang-Otomo; Rienk van Grondelle
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Dec. 2015, [Reviewed]

Thermal Adaptability of the Light-Harvesting Complex 2 from Thermochromatium tepidum: Temperature-Dependent Excitation Transfer Dynamics
Ying Shi; Ning-Jiu Zhao; Peng Wang; Li-Min Fu; Long-Jiang Yu; Jian-Ping Zhang; Zheng-Yu Wang-Otomo
JOURNAL OF PHYSICAL CHEMISTRY B, Nov. 2015, [Reviewed]

Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum
Yuuka Kimura; Tomoaki Kawakami; Long-Jiang Yu; Miku Yoshimura; Masayuki Kobayashi; Zheng-Yu Wang-Otomo
FEBS Letters, 03 Jul. 2015, [Reviewed]

The roles of C-terminal residues on the thermal stability and local heme environment of cytochrome c' from the thermophilic purple sulfur bacterium Thermochromatium tepidum
Yukihiro Kimura; Sachiko Kasuga; Masashi Unno; Takashi Furusawa; Shinsuke Osoegawa; Yuko Sasaki; Takashi Ohno; Zheng-Yu Wang-Otomo
PHOTOSYNTHESIS RESEARCH, Apr. 2015, [Reviewed]

Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum
Anne-Marie Carey; Kirsty Hacking; Nichola Picken; Suvi Honkanen; Sharon Kelly; Dariusz M. Niedzwiedzki; Robert E. Blankenship; Yuuki Shimizu; Zheng-Yu Wang-Otomo; Richard J. Cogdell
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Nov. 2014, [Reviewed]

Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 angstrom
Satomi Niwa; Long-Jiang Yu; Kazuki Takeda; Yu Hirano; Tomoaki Kawakami; Zheng-Yu Wang-Otomo; Kunio Miki
NATURE, Apr. 2014, [Reviewed]

Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 angstrom
Satomi Niwa; Long-Jiang Yu; Kazuki Takeda; Yu Hirano; Tomoaki Kawakami; Zheng-Yu Wang-Otomo; Kunio Miki
NATURE, Apr. 2014, [Reviewed]

1P255 Thermodynamic analysis of metal-protein interaction in the light-harvesting 1 reaction center complex from purple sulfur bacteria(18B. Photobiology:Photosynthesis,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Kimura Yukihiro; Hayashi Yusuke; Otomo Seiu; Ohno Takashi, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2014

1P253 Isotope-edited ATR-FTIR analysis of the light-harvesting 1 reaction center complex from thermophilic purple photosynthetic bacteria(18B. Photobiology:Photosynthesis,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Yura Yuki; Kimura Yukihiro; Otomo Seiu; Ohno Takashi, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2014

ATR-FTIR Detection of Metal-Sensitive Structural Changes in the Light-Harvesting 1 Reaction Center Complex from the Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum
Yong Li; Yukihiro Kimura; Teruhisa Arikawa; Zheng-Yu Wang-Otomo; Takashi Ohno
BIOCHEMISTRY, Dec. 2013, [Reviewed]

Structure analysis and characterization of the cytochrome c-554 from thermophilic green sulfur photosynthetic bacterium Chlorobaculum tepidum
Long-Jiang Yu; Masaki Unno; Yukihiro Kimura; Kasumi Yanagimoto; Hirozo Oh-oka; Zheng-Yu Wang-Otomo
PHOTOSYNTHESIS RESEARCH, Dec. 2013, [Reviewed]

Strontium ions are functionally replaceable with calcium ions in the light-harvesting 1 reaction center core complex from thermophilic purple sulfur bacterium thermochromatium tepidum
Yukihiro Kimura; Yuta Inada; Longjiang Yu; Takashi Ohno; Zhengyu Wang, Springer
Advanced Topics in Science and Technology in China, 2013

Structure Analysis and Comparative Characterization of the Cytochrome c ' and Flavocytochrome c from Thermophilic Purple Photosynthetiic Bacterium Thermochromatium tepidum
Yu Hirano; Yukihiro Kimura; Hideaki Suzuki; Kunio Miki; Zheng-Yu Wang
BIOCHEMISTRY, Aug. 2012, [Reviewed]

Structure Analysis and Comparative Characterization of the Cytochrome c ' and Flavocytochrome c from Thermophilic Purple Photosynthetiic Bacterium Thermochromatium tepidum
Yu Hirano; Yukihiro Kimura; Hideaki Suzuki; Kunio Miki; Zheng-Yu Wang
BIOCHEMISTRY, Aug. 2012, [Reviewed]

Metal cations modulate the bacteriochlorophyll-protein interaction in the light-harvesting 1 core complex from Thermochromatium tepidum
Yukihiro Kimura; Yuta Inada; Tomoko Numata; Teruhisa Arikawa; Yong Li; Jian-Ping Zhang; Zheng-Yu Wang; Takashi Ohno
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2012, [Reviewed]

Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum
Fumie Sekine; Kentaro Horiguchi; Yasuhiro Kashino; Yuuki Shimizu; Long-Jiang Yu; Masayuki Kobayashi; Zheng-Yu Wang
PHOTOSYNTHESIS RESEARCH, Mar. 2012, [Reviewed]

3PT234 Hydrogen-bonding interactions between BChl-a and Trp residues in the light-harvestingl complexes from Thermochro-matium tepidum(The 50th Annual Meeting of the Biophysical Society of Japan)
Li Yong; Kimura Yukihiro; Numata Tomoko; Inada Yuta; Arikawa Teruhisa; Otomo Seiu; Ohno Takashi, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2012

3PT233 Molecular mechanisms for the thermostabilization of cytochrome c' from the thermophilic purple sulfur bacterium Thermochromatium tepidum(The 50th Annual Meeting of the Biophysical Society of Japan)
Kasuga Sachiko; Kimura Yukihiro; Furusawa Kei; Ohno Takashi; Otomo Seiu, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2012

Effects of Aggregation on the Excitation Dynamics of LH2 from Thermochromatium tepidum in Aqueous Phase and in Chromatophores
Fan Yang; Long-Jiang Yu; Peng Wang; Xi-Cheng Ai; Zheng-Yu Wang; Jian-Ping Zhang
JOURNAL OF PHYSICAL CHEMISTRY B, Jun. 2011, [Reviewed]

A Spectroscopic Variant of the Light-Harvesting 1 Core Complex from the Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum
Yukihiro Kimura; Yuta Inada; Long-Jiang Yu; Zheng-Yu Wang; Takashi Ohno
BIOCHEMISTRY, May 2011, [Reviewed]

Examination of the putative Ca2+-binding site in the light-harvesting complex 1 of thermophilic purple sulfur bacterium Thermochromatium tepidum
Long-Jiang Yu; Shogo Kato; Zheng-Yu Wang
PHOTOSYNTHESIS RESEARCH, Dec. 2010, [Reviewed]

Excitation Dynamics of the Light-Harvesting Complex 2 from Thermochromatium Tepidum
Yang Fan; Yu Long-Jiang; Wang Peng; Ai Xi-Cheng; Wang Zheng-Yu; Zhang Jian-Ping
ACTA PHYSICO-CHIMICA SINICA, Jul. 2010, [Reviewed]

Crystal Structure of the Electron Carrier Domain of the Reaction Center Cytochrome c(z) Subunit from Green Photosynthetic Bacterium Chlorobium tepidum
Yu Hirano; Makoto Higuchi; Chihiro Azai; Hirozo Oh-oka; Kunio Miki; Zheng-Yu Wang
JOURNAL OF MOLECULAR BIOLOGY, Apr. 2010, [Reviewed]

2P288 Biosynthetic Replacement of Ca with Sr in pigment-protein complexes from thermophilic Purple Sulfur Bacterium Thermochromatium tepidum(The 48th Annual Meeting of the Biophysical Society of Japan)
Kimura Yukihiro; Inada Yuta; Kasuga Sachiko; Sasaki Yuko; Yu Long-Jiang; Wang Zheng-Yu; Ohno Takashi, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2010

Overexpression, characterization, and crystallization of the functional domain of cytochrome c(z) from Chlorobium tepidum
Makoto Higuchi; Yu Hirano; Yukihiro Kimura; Hirozo Oh-oka; Kunio Miki; Zheng-Yu Wang
PHOTOSYNTHESIS RESEARCH, Oct. 2009, [Reviewed]

Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling
Fei Ma; Yukihiro Kimura; Long-Jiang Yu; Peng Wang; Xi-Cheng Ai; Zheng-Yu Wang; Jian-Ping Zhang
FEBS JOURNAL, Mar. 2009, [Reviewed]

On the excitation-trap dynamics, red absorption and thermal stability of the lh1-rc complex from photosynthetic bacterium thermochromatium tepidum　　　　　　　　　　　　　　　
Fei Ma; Yukihiro Kimura; Long-Jiang Yu; Peng Wang; Xi-Cheng Ai; Zheng-Yu Wang; Jian-Ping Zhang
Optics InfoBase Conference Papers, 2009

Calcium Ions Are Required for the Enhanced Thermal Stability of the Light-harvesting-Reaction Center Core Complex from Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum
Yukihiro Kimura; Long-Jiang Yu; Yu Hirano; Hiroaki Suzuki; Zheng-Yu Wang
JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2009, [Reviewed]

Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum
Fei Ma; Yukihiro Kimura; Xiao-Hui Zhao; Yi-Shi Wu; Peng Wang; Li-Min Fu; Zheng-Yu Wang; Jian-Ping Zhang
BIOPHYSICAL JOURNAL, Oct. 2008, [Reviewed]

Calcium ions are involved in the unusual red shift of the light-harvesting 1 Q(y) transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum
Yukihiro Kimura; Yu Hirano; Long-Jiang Yu; Hiroaki Suzuki; Masayuki Kobayashi; Zheng-Yu Wang
JOURNAL OF BIOLOGICAL CHEMISTRY, May 2008, [Reviewed]

Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum.
Hiroaki Suzuki; Yu Hirano; Yukihiro Kimura; Shinichi Takaichi; Masayuki Kobayashi; Kunio Miki; Zheng-Yu Wang, A light-harvesting-reaction center (LH1-RC) core complex has been highly purified from a thermophilic purple sulfur bacterium, Thermochromatium tepidum. The bacteriochlorophyll (BChl) a molecules in the LH1 exhibit a Q(y) transition at 914 nm, more than 25 nm red-shift from those of its mesophilic counterparts. The LH1-RC complex was isolated in a monomeric form as confirmed by sucrose density gradient centrifugation, blue native PAGE and size-exclusion chromatography. Four subunits (L, M, H and a tetraheme cytochrome) in RC and two polypeptides (alpha and beta) in LH1 were identified. Spirilloxanthin was determined to be the predominant carotenoid in the core complex. The purified core complex was highly stable, no significant change in the LH1 Q(y) transition was observed over 10 days of incubation at room temperature in dark. Circular dichroism spectrum of the LH1 complex was characterized by low intensity and nonconservative spectral shape, implying a high symmetry of the large LH1 ring and interaction between the BChl a and carotenoid molecules. A dimeric feature of the BChl a molecules in LH1 was revealed by magnetic circular dichroism spectrum. Crystals of the core complex were obtained which diffracted X-rays to about 10 A.
Biochimica et biophysica acta, Aug. 2007, [Reviewed]

Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum
Hiroaki Suzuki; Yu Hirano; Yukihiro Kimura; Shinichi Takaichi; Masayuki Kobayashi; Kunio Miki; Zheng-Yu Wang
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Aug. 2007, [Reviewed]

Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum
Hiroaki Suzuki; Yu Hirano; Yukihiro Kimura; Shinichi Takaichi; Masayuki Kobayashi; Kunio Miki; Zheng-Yu Wang
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Aug. 2007

Solution structure of the Rhodobacter sphaeroides PufX membrane protein: Implications for the quinone exchange and protein-protein interactions
Zheng-Yu Wang; Hiroaki Suzuki; Masayuki Kobayashi; Tsunenori Nozawa
BIOCHEMISTRY, Mar. 2007, [Reviewed]

Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli
Shiho Onodera; Hiroaki Suzuki; Yuichiro Shimada; Masayuki Kobayashi; Tsunenori Nozawa; Zheng-Yu Wang
PHOTOCHEMISTRY AND PHOTOBIOLOGY, Jan. 2007, [Reviewed]

2P344 Roles of calcium ions on the structural stability of the LH1-RC core complex from thermophilic purple bacterium Thermochromatium tepidum(43. Photosynthesis,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Kimura yukihiro; Suzuki Hiroaki; Dao rina; Nakamura Masao; Otomo Seiu, The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2006

Isotopic labeling of proteins by utilizing photosynthetic bacteria
H Suzuki; Y Shimada; M Kobayashi; M Kudo; T Nozawa; ZY Wang
ANALYTICAL BIOCHEMISTRY, Dec. 2005, [Reviewed]

Electronic properties and thermal stability of soluble redox proteins from a thermophilic purple sulfur photosynthetic bacterium, Thermochromatium tepidum
M Kobayashi; T Saito; K Takahashi; ZY Wang; T Nozawa
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, Dec. 2005, [Reviewed]

Selective detection of the solid-state NMR signals from the bacteriochlorophyll a dimers in a reconstituted light-harvesting 1 complex
M Umetsu; T Kadota; ZY Wang; Y Tanaka; T Adschiri; T Nozawa
CHEMISTRY LETTERS, Jul. 2005, [Reviewed]

Reconstitution of photosynthetic reaction centers and core antenna-reaction center complexes in liposomes and their thermal stability
M Kobayashi; Y Fujioka; T Mori; M Terashima; H Suzuki; Y Shimada; T Saito; ZY Wang; T Nozawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jun. 2005, [Reviewed]

Solution structures of the core light-harvesting alpha and beta polypeptides from Rhodospirillum rubrum: Implications for the pigment-protein and protein-protein interactions
ZY Wang; K Gokan; M Kobayashi; T Nozawa
JOURNAL OF MOLECULAR BIOLOGY, Mar. 2005

3P122 Examination of the solution structures between native and E. coli -expressed lightharvesting membrane proteins
Otomo S.; Mochizuki Y.; Shimada Y.; Suzuki H., The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2005

Functional expression and characterization of a bacterial light-harvesting membrane protein in Escherichia coli and cell-free synthesis systems
Y Shimada; ZY Wang; Y Mochizuki; M Kobayashi; T Nozawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2004, [Reviewed]

NMR relaxation study of the bacteriochlorophyll c in solutions
ZY Wang; T Kadota; M Kobayashi; A Kasuya; T Nozawa
JOURNAL OF PHYSICAL CHEMISTRY B, Sep. 2004

Magic-angle spinning nuclear magnetic resonance under ultrahigh field reveals two forms of intermolecular interaction within CH2Cl2-treated (3(1)R)-type bacteriochlorophyll c solid aggregate
M Umetsu; JG Hollander; J Matysik; ZY Wang; T Adschiri; T Nozawa; HJM de Groot
JOURNAL OF PHYSICAL CHEMISTRY B, Feb. 2004, [Reviewed]

1P119 Structural analysis of bacterial light-harvesting membrane proteins
Otomo S.; Gokan K.; Kobayashi M.; Nozawa T., The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2004

Dynamic exchange properties of the antiparallel bacteriochlorophyll c dimers
M Umetsu; R Seki; T Kadota; ZY Wang; T Adschiri; T Nozawa
JOURNAL OF PHYSICAL CHEMISTRY B, Sep. 2003, [Reviewed]

Determination of the B820 subunit size of a bacterial core light-harvesting complex by small-angle neutron scattering
Wang, Z. Y.; Muraoka, Y.; Nagao, M.; Shibayama, M.; Kobayashi, M.; Nozawa, T.
Biochemistry, 2003, [Reviewed]

Purification and characterization of the polypeptides of core light-harvesting complexes from purple sulfur bacteria
WANG Z.-Y.
Photosynth. Res., 2003

Circular and magnetic circular dichroism studies of bacteriochlorophyll c aggregates: T-shaped and antiparallel dimers
Mitsuo Umetsu; Ryoichi Seki; Zheng-Yu Wang; Izumi Kumagai; Tsunenori Nozawa
Journal of Physical Chemistry B, 18 Apr. 2002, [Reviewed]

Interaction of photosynthetic pigments with various organic solvents 2. Application of magnetic circular dichroism to bacteriochlorophyll a and light-harvesting complex 1
M Umetsu; ZY Wang; K Yoza; M Kobayashi; T Nozawa
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Apr. 2000, [Reviewed]

The structure for thermostability of thermophilic photosynthetic reaction center
Mori T.; Kobayashi M.; Nogi T.; Wang Z.-Y.; Miki K.; Nozawa T., The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 2000

13C- and 15N-NMR studies on the intact bacteriochlorophyll c dimers in solutions
Zheng-Yu Wang; Mitsuo Umetsu; Masayuki Kobayashi; Tsunenori Nozawa
Journal of the American Chemical Society, 13 Oct. 1999, [Reviewed]

Complete Assignment of 1H NMR Spectra and Structural Analysis of Intact Bacteriochlorophyll C Dimer in Solution
Zheng-Yu Wang; Mitsuo Umetsu; Masayuki Kobayashi; Tsunenori Nozawa
The Journal of Physical Chemistry B, Apr. 1999, [Reviewed]

Interaction of photosynthetic pigments with various organic solvents. Magnetic circular dichroism approach and application to chlorosomes
Mitsuo Umetsu; Zheng-Yu Wang; Masayuki Kobayashi; Tsunenori Nozawa
Biochimica et Biophysica Acta - Bioenergetics, 27 Jan. 1999, [Reviewed]

CP/MAS ^<13>C NMR spectra of the relatively ordered R[E, E]BChl c high aggregates
Umetsu M.; Wang Z.-Y.; Yamauchi K.; Kobayashi M.; Nozawa T., The Biophysical Society of Japan General Incorporated Association
Seibutsu Butsuri, 1999

Interaction of photosynthetic pigments with various organic solvents as revealed from magnetic circular dichroism measurements
M Umetsu; ZY Wang; M Kobayashi; T Nozawa
PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, 1998, [Reviewed]

A small-angle neutron scattering study on the small aggregates of bacteriochlorophylls in solutions
Zheng-Yu Wang; Mitsuo Umetsu; Kenji Yoza; Masayuki Kobayashi; Masayuki Imai; Yushu Matsushita; Nobuo Niimura; Tsunenori Nozawa
Biochimica et Biophysica Acta - Bioenergetics, 16 May 1997, [Reviewed]

New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1',2'-dihydro-γ-carotene, 1',2'-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains
Shinichi Takaichi; Zheng-Yu Wang; Mitsuo Umetsu; Tsunenori Nozawa; Keizo Shimada; Michael T. Madigan
Archives of Microbiology, 1997, [Reviewed]

Molecular structures and optical properties of aggregated forms of chlorophylls analyzed by means of magnetic circular dichroism
Masayuki Kobayashi; Zheng-Yu Wang; Kenji Yoza; Mitsuo Umetsu; Hideo Konami; Mamuro Mimuro; Tsunenori Nozawa, Elsevier
Spectrochimica Acta - Part A Molecular Spectroscopy, 1996, [Reviewed]

Morphology and spectroscopy of chlorosomes from Chlorobium tepidum by alcohol treatments
Zheng-Yu Wang; Giselher Marx; Mitsuo Umetsu; Masayuski Kobayashi; Mamoru Mimuro; Tsunenori Nozawa
BBA - Bioenergetics, 12 Dec. 1995, [Reviewed]

MAGNETIC CIRCULAR-DICHROISM INVESTIGATION ON CHROMOPHORES IN REACTION CENTERS OF PHOTOSYSTEM-I AND PHOTOSYSTEM-II OF GREEN PLANT PHOTOSYNTHESIS
T NOZAWA; M KOBAYASHI; ZY WANG; S ITOH; M IWAKI; M MIMURO
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Jan. 1995, [Reviewed]

Molecular networks and funneling process of energy transfer in green photosynthetic bacteria
M Mimuro; K Matsuura; K Shimada; Y Nishimura; Yamazaki, I; M Kobayashi; ZY Wang; T Nozawa
PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL I, 1995, [Reviewed]

好熱性紅色光合成細菌Halorhodospira halochlorisの特性解析　　　　　　　　　　　　　　　
能島紳吾; 小林正幸; 竹中慎治; M.T.Madaigan; 大友征宇; 木村行宏
日本農芸化学会 関西・中部支部2019合同神戸大会（神戸）, Sep. 2019

紅色光合成細菌電子伝達系におけるキノン輸送の追跡　　　　　　　　　　　　　　　
岸利華子; 今西三千絵; 小林正幸; 永島賢治; 竹中慎治; M.T.Madaigan; 大友征宇; 木村行宏
日本農芸化学会 関西・中部支部2019合同神戸大会（神戸）, Sep. 2019

紅色光合成細菌におけるキノン動態の解析　　　　　　　　　　　　　　　
岸利華子; 今西三千絵; 小林正幸; 永島賢治; 竹中慎治; M.T.Madaigam; 大友征宇; 木村行宏
第27回「光合成セミナー2019：反応中心と色素系の多様性」（豊中）, Jul. 2019

好熱性および好塩性を示す Halorhodospira halochloris由来光捕集反応中心複合体の特性解析　　　　　　　　　　　　　　　
能島紳吾; 小林正幸; 竹中慎治; Michael; T. Madigan; 大友征宇; 木村行宏
第26回「光合成セミナー2018：反応中心と色素系の多様性」（神戸）, Jul. 2018

光誘起FTIR分光法を用いた紅色細菌由来LH1-RC複合体におけるキノン分子の追跡　　　　　　　　　　　　　　　
岸利華子; 今西三千絵; 小林正幸; 永島賢治; Michael T. Madigan; 大友征宇; 木村 行宏
第26回「光合成セミナー2018：反応中心と色素系の多様性」（神戸）, Jul. 2018

紅色光合成細菌 Thiorhodovibrio Strain 970が示す異常なLH1Qyレッドシフトの起源　　　　　　　　　　　　　　　
今西三千絵; 竹之内瑠貴; 小林正幸; 竹中慎治; ࣭Michael T. Madigan; Jorg Overmann; 大友征宇; 木村 行宏
第26回「光合成セミナー2018：反応中心と色素系の多様性」（神戸）, Jul. 2018

Monitoring of quinone reduction in the thermophilic purple bacterium Thermochromatium tedium by means of isotope-edited FTIR spectroscopy　　　　　　　　　　　　　　　
今西 三千絵; 岸 利華子; 小林 正幸; 大友 征宇; 木村 行宏
第55回日本生物物理学会年会（熊本）, Sep. 2017

同位体標識化された好熱性紅色細菌Thermochromatium tediumにおけるキノール分子の振動分光学的検出　　　　　　　　　　　　　　　
岸利華子; 今西三千絵; 小林正幸; 大野 隆; 大友征宇; 木村 行宏
第25回「光合成セミナー2017：反応中心と色素系の多様性」（神戸）, Jul. 2017

FTIR 分光法を用いた好熱性紅色細菌 Thermochromatium tepidumにおける光合成電子伝達反応のモニタリング　　　　　　　　　　　　　　　
今西三千絵; 橋本佳奈子; 永島賢治; 小林正幸; 由良優季; 大野 隆; 大友征宇; 木村 行宏
第58回日本植物生理学会年会, Mar. 2017

ハイブリッド型光捕集反応中心複合体の機能解析
後藤健太; 佐々木舞; 阿部悠人; 小沼里沙; 永島賢治; 永島咲子; 大友征宇
生体分子科学討論会講演要旨集, 2017

好熱性紅色光合成細菌Thermochromatium tepidum 由来反応中心複合体におけるカルシウムイオンの機能的、構造的役割　　　　　　　　　　　　　　　
Michie Imanishi; Masayuki Kobayashi; Manami Kobayashi; Mari Matsuzaki; Yuki Yura; Takashi Ohno; Seiu Otomo; Yukihiro Kimura
第54回日本生物物理学会年会（東京）, Nov. 2016

光合成細菌，光合成膜を用いた光電変換特性について　　　　　　　　　　　　　　　
小林正幸; 高木洋介; 豊福航平; 大友征宇; 野澤庸則
第21回「光合成セミナー2013：光合成の色素系と反応中心に関するセミナー」（名古屋）, Jun. 2013

光合成膜顆粒懸濁液を用いた光電変換特性　　　　　　　　　　　　　　　
小林正幸; 高木洋介; 野沢庸則; 大友征宇
第19回光合成の色素系と反応中心に関するセミナー（大阪）, Jul. 2011

紅色光合成細菌の光捕集複合体LH2の特性評価　　　　　　　　　　　　　　　
関根文恵; 堀口健太郎; 菓子野康浩; 清水佑記; 于龍江; 小林正幸; 大友征宇
第19回光合成の色素系と反応中心に関するセミナー（大阪）, Jul. 2011

好熱性紅色光合成細菌Thermochromatium tepidumシトクロム複合体の単離精製　　　　　　　　　　　　　　　
小林正幸; Y.Xin; A.M.Collin; J.Wen; P.Bell; 齊藤貴之; 大友征宇; R. E. Blankenship
第18回光合成の色素系と反応中心に関するセミナー（京都）, Jul. 2010

Optical properties of light-harvesting complex 2 from thermophilic purple sulfur bacterium, Thermochromatium tepidum　　　　　　　　　　　　　　　
小林正幸; 鈴木宏昭; 大友征宇; 野澤庸則
13th International Symposium on Phototrophic Prokaryotes, Aug. 2009

Isolations of reaction center and antenna complexes from photosynthetic bacteria
大友 征宇; 小林 正幸; 大岡 宏造
Low temperature science, 2008

Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction centers.
Masayuki Kobayashi; Atsuo Takaya; Nobuyuki Kanai; Yosuke Ota; Takayuki Saito; Zheng-Yu Wang; Tsunenori Nozawa
Journal of biochemistry, Sep. 2004, [Reviewed]

Probing the Transmembrane Potential of Bacterial Cells by Voltage-Sensitive Dyes
Hiroaki SUZUKI; Zheng-Yu WANG; Mie YAMAKOSHI; Masayuki KOBAYASHI; Tsunenori NOZAWA
The Japan Society for Analytical Chemistry ANALYTICAL SCIEMCES, 2003

A dimeric structure of bacteriochlorophyllide c molecules studied by scanning tunneling microscopy
Qing-Min Xu; Li-Jun Wan; Shu-Xia Yin; Chen Wang; Chun-Li Bai; Takasada Ishii; Kaku Uehara; Zheng-Yu Wang; Tsunenori Nozawa
Journal of Physical Chemistry B, 28 Mar. 2002

N-terminal methylation of the core light-harvesting complex in purple photosynthetic bacteria
WANG Z.-Y.
FEBS Lett., 2002

Selective detection and assignment of the solution NMR signals of bacteriochlorophyll a in a reconstituted subunit of a light-harvesting complex
WANG Z.-Y.
J. Am. Chem. Soc., 2002

Combination of conditioned medium and elicitation enhances taxoid production in bioreactor cultures of Taxus chinensis cells
WANG Z. Y.
Biochem. Eng. J., 2002

The dimerization of folded monomers of ribulose 1,5-bisphosphate carboxylase/oxygenase
S Luo; ZY Wang; M Kobayashi; T Nozawa
JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2001

Re-identification of the N-terminal amino acid residue and its modification of beta-polypeptide of light-harvesting complex I from Rhodospirillum rubrum
ZY Wang; M Shimonaga; Y Muraoka; M Kobayashi; T Nozawa
PHOTOSYNTHESIS RESEARCH, 2001

Methionine oxidation and its effect on the stability of reconstituted subunit of light-harvesting complex from Rhodospirillum rubrum
WANG Z.-Y.
Eur. J. Biochem., 2001

Molecular assembly of covalently-linked mesoporphyrin dimers with light-harvesting polypeptides
A Kashiwada; Y Takeuchi; H Watanabe; T Mizuno; H Yasue; K Kitagawa; K Iida; ZY Wang; T Nozawa; H Kawai; T Nagamura; Y Kurono; M Nango
TETRAHEDRON LETTERS, Mar. 2000

How the formation process influences the structure of BChl c aggregates
M Umetsu; ZY Wang; J Zhang; T Ishii; K Uehara; Y Inoko; M Kobayashi; T Nozawa
PHOTOSYNTHESIS RESEARCH, May 1999

Biochemical and spectral characterization of the core light harvesting complex 1 (LH1) from the thermophilic purple sulfur bacterium Chromatium tepidum
Fathir, I; M Ashikaga; K Tanaka; T Katano; T Nirasawa; M Kobayashi; ZY Wang; T Nozawa
PHOTOSYNTHESIS RESEARCH, Nov. 1998

In situ measurements of ribulose-1,5-bisphosphate carboxylase activity by nuclear magnetic resonance
ZY Wang; S Luo; K Sato; M Kobayasi; T Nozawa
ANALYTICAL BIOCHEMISTRY, Mar. 1998

Measurements of the CO2/O2 specificity of ribulose 1,5-bisphosphate carboxylase/oxygenase by 31P- and 1H-NMR
Zheng-Yu Wang; Shen Luo; Kazuhiro Sato; Masayuki Kobayashi; Tsunenori Nozawa
Photosynthesis Research, 1998

The genes coding for the L, M and cytochrome subunits of the photosynthetic reaction center from the thermophilic purple sulfur bacterium Chromatium tepidum
Fathir, I; K Tanaka; K Yoza; A Kojima; M Kobayashi; ZY Wang; F Lottspeich; T Nozawa
PHOTOSYNTHESIS RESEARCH, Jan. 1997

Size distribution and photophosphorylation of chromatophores from Rhodospirillum rubrum
ZY Wang; M Kudoh; Y Ohama; H Nakatani; M Kobayashi; T Nozawa
PHOTOSYNTHESIS RESEARCH, Jan. 1997

High-resolution solid-state 13C NMR of the LH1 from Rhodospirillum rubrum
Kenji Yoza; Zheng-Yu Wang; Masayuki Kobayashi; Tsunenori Nozawa
Photosynthesis Research, 1997

Excitation energy transfer in the green photosynthetic bacterium Chloroflexus aurantiacus: A specific effect of 1-hexanol on the optical properties of baseplate and energy transfer processes
M Mimuro; Y Nishimura; Yamazaki, I; M Kobayashi; ZY Wang; T Nozawa; K Shimada; K Matsuura
PHOTOSYNTHESIS RESEARCH, May 1996

A SMALL-ANGLE X-RAY-SCATTERING STUDY OF ALGINATE SOLUTION AND ITS SOL-GEL TRANSITION BY ADDITION OF DIVALENT-CATIONS
ZY WANG; JW WHITE; M KONNO; S SAITO; T NOZAWA
BIOPOLYMERS, Feb. 1995

Magnetic circular dichroism investigation on chromophores in reaction centers of photosystem I and II of green plant photosynthesis
Tsunenori Nozawa; Masayuki Kobayashi; Zheng-Yu Wang; Shigeru Itoh; Masayo Iwaki; Mamoru Mimuro; Kimiyuki Satoh
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 1995

STRUCTURES OF CHLOROSOMES AND AGGREGATED BCHL-C IN CHLOROBIUM-TEPIDUM FROM SOLID-STATE HIGH-RESOLUTION CP/MAS C-13 NMR
T NOZAWA; K OHTOMO; M SUZUKI; H NAKAGAWA; Y SHIKAMA; H KONAMI; ZY WANG
PHOTOSYNTHESIS RESEARCH, Jul. 1994