Yoshihito SUZUKIProfessor
■Researcher basic information
Organization
Educational Background
Career
Member History
- Nov. 2024 - Present, 監事, 一般社団法人 植物化学調節学会
- Oct. 2016 - Present, 代議員, 植物化学調節学会
- Nov. 2020 - Nov. 2024, 代表理事，会長, 一般社団法人植物化学調節学会
- Feb. 2023 - Mar. 2024, オープンイノベーション研究・実用化推進事業評議委員, 国立研究開発法人農業・食品産業技術総合研究機構 生物系特定産業技術研究支援センター
- Jan. 2021 - Mar. 2022, 評議委員, 国立研究開発法人農業・食品産業技術総合研究機構
- Jan. 2017 - Feb. 2021, 英文誌編集委員長, 日本農芸化学会
- Apr. 2012 - Feb. 2021, 英文誌総務会委員, 日本農芸化学会
- Oct. 2016 - Nov. 2020, 理事（副会長）、学会賞選考委員長, 植物化学調節学会
- Aug. 2018 - Jun. 2019, 特別研究員等審査会専門委員，卓越研究員候補者選考委員会書面審査員および国際事業委員会書面審査員・書面評価員, 独立行政法人日本学術振興会
- May 2015 - May 2019, 英文誌編集理事, 日本農芸化学会
- Mar. 2019 - Mar. 2019, イノベーション創出強化研究推進事業評議委員, 国立研究開発法人農業・食品産業技術総合研究機構
- Jul. 2016 - Feb. 2019, 農芸化学女性研究者賞等授賞選考委員, 日本農芸化学会
- Mar. 2015 - Feb. 2019, 男女共同参画委員, 日本農芸化学会
- Feb. 2017 - Mar. 2018, 1次（書面）審査専門評価委員, 農林水産業・食品産業科学技術研究推進事業
- Jan. 2012 - May 2017, 役員候補者等選考委員会委員, 日本農芸化学会
- Apr. 2001 - Sep. 2016, 会計幹事, 植物化学調節学会
- Feb. 2015 - Mar. 2016, 一次（書面）審査専門評価委員, 農林水産業・食品産業科学技術研究推進事業
- Apr. 2013 - Feb. 2015, 学術活動強化委員会委員, 日本農芸化学会
- Apr. 2012 - Feb. 2015, 英文紙編集委員, 日本農芸化学会
- Apr. 2012 - Mar. 2013, 関東支部幹事, 日本農芸化学会
- Mar. 2012 - Mar. 2012, 書類審査専門委員, 農林水産省「イノベーション創出基礎的研究推進事業」
- Sep. 2011 - Mar. 2012, 専門委員, 農業・食品産業技術総合研究機構競争的資金事業
- Dec. 2009 - Nov. 2011, 科学研究費委員会専門委員, 日本学術振興会
- Apr. 2001 - Sep. 2009, 農学会運営委員, 植物化学調節学会
- Apr. 2005 - Mar. 2007, 和文誌編集委員, 日本農芸化学会
- Apr. 2005 - Mar. 2007, 関東支部幹事, 日本農芸化学会
- Jan. 2002 - Mar. 2005, 和文誌編集幹事, 日本農芸化学会
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Message from Researchers
(Message from Researchers)
(教員からのメッセージ)
学部4年生から博士課程修了まで東京大学農学部の高橋信孝先生の指導のもとに研究を行いました。その研究室は植物ホルモンの1つであるジベレリンの発見からの歴史を持つ研究室で，私も植物ホルモンに代表される植物の生理活性物質に関心を持っています。この分野の研究に関して，分子生物学，遺伝学，その他様々な手法を用いた研究を行ってきましたが，その経験を生かして，今後は植物の生長や分化を制御する新たな生理活性物質を見出すような研究を行っていきたいと考えています。特に化学生態学分野の研究として，植物以外の生物によって植物の生長や分化などが影響を受ける現象について，そのメカニズムの解明を行いたいと思っています。最近はおもにゴール（虫えい）の研究をおこなっています。虫えいは，植物に寄生する虫が植物組織を自分の都合の良い形に変形させたもので，虫はそこに住み着いて食糧としています。とてもドラマティックな現象で，この形成に関わる機構やゴール組織の性状を明らかにしたいと思っています。最近，ゴール形成昆虫が植物ホルモンであるオーキシンやサイトカイニンを生合成することを見いだしました。これらの植物ホルモンがゴール形成に重要な働きをしていると考えています。現在は，植物ホルモン類の生合成の仕組みの解明を行っています。また，どのようにして様々な面白い形のゴールが形成されるのかを知りたいと考えています。組織学的な観察，遺伝子発現制御など様々な観点からのアプローチを考え，検討を加えています。
■Research activity information
Award
Paper
- A unique substrate specificity of PonAAS2, an aromatic aldehyde synthase, involved in phytohormone auxin biosynthesis in a gall-inducing sawfly Euura sp. “Pontania”
Yoshihito Suzuki; Hikaru Ichikawa; Yuri Kunioka; Umi Miyata; Shugo Nakamura; Zui Fujimoto, Lead
Bioscience, Biotechnology, and Biochemistry, 24 Mar. 2025, [Reviewed] - Conferring High IAA Productivity on Low-IAA-Producing Organisms with PonAAS2, an Aromatic Aldehyde Synthase of a Galling Sawfly, and Identification of Its Inhibitor
Takeshi Hiura; Hibiki Yoshida; Umi Miyata; Tadao Asami and Yoshihito Suzuki, Last, The gall-inducing sawfly (Pontania sp.) possesses high concentrations of indoleaceticacid (IAA; the active form of the phytohormone auxin), which may play an importantrole in gall induction. Among the insect aromatic aldehyde synthases (AASs) studied to date, sawfly PonAAS2 is the only AAS involved in IAA biosynthesis that produces indoleacetaldehyde from Trp. In this study, we show that the introduction of the PonAAS2 gene is able to confer high IAA productivity on other organisms, using Caenorhabditis elegans as the model system. We also identified a specific inhibitor of PonAAS2., MDPI
Insects, 02 Jul. 2023, [Reviewed] - Terrestrial arthropods broadly possess endogenous phytohormones auxin and cytokinins
M. Tokuda; Y. Suzuki; S. Fujita; H. Matsuda; S. Adachi‐Fukunaga & A. K. Elsayed, Some herbivorous insects possess the ability to synthesize phytohormones and are considered to use them for manipulating their host plants, but how these insects acquired the ability remains unclear. We investigated endogenous levels of auxin (IAA) and cytokinins (iP and tZ), including their ribosides,(iPR and tZR), in various terrestrial arthropod taxa. Surprisingly, IAA was detected in all arthropods analysed. In contrast, tZ and/or tZR was detected only in some taxa. Endogenous levels of IAA were not significantly different among groups with different feeding habits, but gall inducers possessed significantly higher levels of iPR, tZ and tZR. Ancestral state reconstruction of the ability to synthesize tZ and tZR revealed that the trait has only been acquired in taxa containing gall inducers. Our results strongly suggest critical role of the cytokinin synthetic ability in the evolution of gall-inducing habit and IAA has some function in arthropods.
Scentific Reports, Mar. 2022, [Reviewed] - Identification of an aromatic aldehyde synthase involved in indole-3-acetic acid biosynthesis in the galling sawfly (Pontania sp.) and screening of an inhibitor
Umi Miyata; Kenta Arakawa; Mami Takei; Tadao Asami; Kazuya Asanbou; Hiroaki Toshima; Yoshihito Suzuki, Last, Indole-3-acetic acid (IAA), a phytohormone auxin, may be involved in insect gall induction. We previously proposed that the IAA biosynthetic pathway is Trp → indole-3-acetaldoxime → indole-3-acetaldehyde (IAAld) → IAA or Trp → IAAld → IAA. In this study, we surveyed galling sawfly enzymes responsible for the rate-limiting steps using a heterologous protein expression system and identified PonAAS2, an aromatic aldehyde synthase, that catalyzed the conversion of Trp to IAAld. The PonAAS2 gene was highly expressed in early- and mid-stage larvae that contained high concentrations of IAA, but the expression level was almost negligible in larvae that had escaped from their gall in autumn and contained very low concentrations of IAA. An inhibitor of PonAAS2, obtained by screening a chemical library, inhibited IAA production in sawfly enzyme solution by 80%, suggesting the important role of this enzyme in IAA biosynthesis in sawfly., Elseview
Insect Biochemistry and Molecular Biology, 21 Aug. 2021, [Reviewed] - Reprograming of the Developmental Program of Rhus javanica During Initial Stage of Gall Induction by Schlechtendalia chinensis
Hirano; T.; Kimura; S.; Sakamoto; A.; Okamoto; A.; Nakayama; T.; Suzuki; Y.; Ohshima; I.
Frontiers in Plant Science, 15 May 2020, [Reviewed] - Quantification of Functional Aromatic Amino Acid Metabolites in Fermented Foods and Their Production by Food Microorganisms
Yuka Kimura; Reiji Aoki; Yoshiharu Takayama; Chise Suzuki; Yoshihito Suzuki, Last, Aromatic amino acid metabolites, including aromatic pyruvates and aromatic lactates, have been shown to be antioxidants with high radical scavenging activity. We surveyed the occurrence and distribution of these metabolites in foods and beverages, focusing on fermented products. Lactic acid bacteria (LAB)-fermented products showed high concentrations of aromatic lactates and much lower concentrations of aromatic pyruvates, while yeast-fermented products showed relatively high levels of aromatic pyruvates. The results were in accordance with the production of aromatic lactates and pyruvates by LAB and Saccharomyces cerevisiae under standard experimental culture conditions. Our findings therefore indicate the importance of utilizing the metabolic activities of fermenting microorganisms for developing foods with beneficial functions., JAPANESE SOC FOOD SCI & TECHNOLOGY
FOOD SCIENCE AND TECHNOLOGY RESEARCH, Jan. 2020, [Reviewed] - Identification of an aldehyde oxidase involved in indole-3-acetic acid synthesis in Bombyx mori silk gland.
Mami Takei; Syota Kogure; Chiaki Yokoyama; Yoshiaki Kouzuma; and Yoshihito Suzuki, Last, Taylors & Francis
Biosci. Biotechnol. Biochem., Jan. 2019, [Reviewed] - Novel tryptophan metabolic pathways in auxin biosynthesis in silkworm
Chiaki Yokoyama; Mami Takei; Yoshiaki Kouzuma; Shinji Nagata; Yoshihito Suzuki, Last, In the course of our study of the biosynthetic pathway of auxin, a class of phytohormones, in insects, we proposed the biosynthetic pathway tryptophan (Trp) -> indole-3-acetaldoxime (IAOx) -> indole-3-acetadehyde (IAA1d) -> indole-3-acetic acid (IAA). In this study, we identified two branches in the metabolic pathways in the silkworm, possibly affecting the efficiency of IAA production: Trp indole-3-pyruvic acid -> indole-3-lactic acid and IAAld -> indole-3-ethanol. We also determined the apparent conversion activities (2.05 x 10(-7) UmL(-1) for Trp -> IAA, 1.30 x 10(-5) U mL(-1) for IAOx -> IAA, and 3.91 x 10(-1) U mL(-1) for IAAld -> IAA), which explain why IAOx and IAAld are barely detectable as either endogenous compounds or metabolites of their precursors. The failure to detect IAAld, even in the presence of an inhibitor of the conversion IAAld -> IAA, is explained by a switch in the conversion from IAAld -> IAA to IAAld -> IEtOH., PERGAMON-ELSEVIER SCIENCE LTD
JOURNAL OF INSECT PHYSIOLOGY, Aug. 2017, [Reviewed] - Life history of Stenopsylla nigricornis (Hemiptera: Psylloidea: Triozidae) and phytohormones involved in its gall induction
Shinya Kai; Shun Kumashiro; Shuhei Adachi; Yoshihito Suzuki; Yoshihisa Shiomi; Kiyoko Matsunaga; Naohisa Gyoutoku; Tadao Asami; Makoto Tokuda, Many phytophagous insects have an ability to manipulate plant tissue and induce galls, but the mechanism is not yet fully understood. Some insects have multivoltine life cycles, and each generation induces galls on different plant species or different organs in the same host. Such host-use patterns are interesting study subjects to clarify the gall-inducing mechanisms of insects. We focused on a multivoltine and gall-inducing psyllid Stenopsylla nigricornis Kuwayama (Hemiptera: Psylloidea: Triozidae), which is associated with Symplocos lucida Sieb. (Symplocaceae). Based on periodic field surveys in Kyushu, Japan, S. nigricornis is revealed to have a bivoltine life history. Then, we revealed that the spring generation induces galls on leaves, while the autumn generation does so on flower buds and overwintering leaf buds. We also analyzed phytohormones in normal plant tissue, S. nigricornis nymphs, and their galls. As a result, nymphs were discovered to contain much higher concentrations of isopentenyladenosine and its possible precursor, isopentenyladenosine riboside than plant tissues, strongly suggesting that the phytohormone is involved in gall induction by S. nigricornis. Because flower bud galls contained significantly lower concentrations of abscisic acid (ABA) than normal flower bud, the autumn generation nymphs are considered to regulate the ABA level and to promote the earlier opening of host flower buds., SPRINGER
ARTHROPOD-PLANT INTERACTIONS, Feb. 2017, [Reviewed] - Transcriptomic characterization of gall tissue of Japanese elm tree (Ulmus davidiana var. japonica) induced by the aphid Tetraneura nigriabdominalis
Mami Takei; Shinsaku Ito; Keisuke Tanaka; Taichiro Ishige; Yoshihito Suzuki, Last, Insect galls are abnormal plant tissues induced by parasitic insect(s) for use as their habitat. In previous work, we suggested that gall tissues induced by the aphid Tetraneura nigriabdominalis on Japanese elm trees are less responsive than leaf tissues to jasmonic acid (JA), which is involved in the production of volatile organic compounds as a typical defensive reaction of plants against attack by insect pests. A comprehensive analysis of gene expression by RNA sequencing indicated that the number of JA responsive genes was markedly lower in gall tissues than in leaf tissues. This suggests that gall tissues are mostly defective in JA signaling, although JA signaling is not entirely compromised in gall tissue. Gene ontology analysis sheds light on some stress-related unigenes with higher expression levels in gall tissues, suggesting that host plants sense aphids as a biotic stress but are defective in the JA-mediated defense response in gall tissues., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2017, [Reviewed] - Eucommicin A, a beta-truxinate lignan from Eucommia ulmoides, is a selective inhibitor of cancer stem cells
Ayaka Fujiwara; Mayuko Nishi; Shigeo Yoshida; Morifumi Hasegawa; Chieko Yasuma; Akihide Ryo; Yoshihito Suzuki, Last, Cancer stem cells (CSCs) constitute a small population of undifferentiated cells within a tumor that have the ability to self-renew and drive tumor formation, thus behaving as cancer-initiating cancer cells. Therapeutic interventions that eliminate CSCs are necessary to completely cure patients, since CSCs are a crucial source of tumor recurrence and metastasis. An induced CSC-like (iCSCL) model was recently established using induced pluripotent stem cells (iPSCs). In this study, a natural product-eucommicin A-was identified from Eucommia ulmoides leaves by screening for anti-CSC activity using the iCSCL model. Its structure was elucidated by spectroscopic methods as a quinic acid diester of 3,4,3',4'-tetrahydroxy-beta-truxinic acid. Eucommicin A exhibited selective anti-CSC activity and inhibited tumor sphere formation by iCSCL cells. The results of this study suggest that eucommicin A could serve as a lead compound in the development of drugs to abrogate the sternness and self-renewal ability of CSCs. (C) 2015 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
PHYTOCHEMISTRY, Feb. 2016, [Reviewed] - Adaptive significance of gall formation for a gall-inducing aphids on Japanese elm trees
Mami Takei; Sayaka Yoshida; Takashi Kawai; Morifumi Hasegawa; Yoshihito Suzuki, Last, Insect galls are abnormal plant tissues induced by external stimuli from parasitizing insects. It has been suggested that the stimuli include phytohormones such as auxin and cytokinins produced by the insects. In our study on the role of hormones in gall induction by the aphid Tetraneura nigriabdominalis, it was found that feedback regulation related to auxin and cytokinin activity is absent in gall tissues, even though the aphids contain higher concentrations of those phytohormones than do plant tissues. Moreover, jasmonic acid signaling appears to be compromised in gall tissue, and consequently, the production of volatile organic compounds, which are a typical defense response of host plants to herbivory, is diminished. These findings suggest that these traits of the gall tissue benefit aphids, because the gall tissue is highly sensitive to auxin and cytokinin, which induce and maintain it. The induced defenses against aphid feeding are also compromised. The abnormal responsiveness to phytohormones is regarded as a new type of extended phenotype of gall-inducing insects. (C) 2014 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
JOURNAL OF INSECT PHYSIOLOGY, Jan. 2015, [Reviewed] - Biosynthetic pathway of the phytohormone auxin in insects and screening of its inhibitors
Hiroyoshi Suzuki; Junpei Yokokura; Tsukasa Ito; Ryoma Arai; Chiaki Yokoyama; Hiroaki Toshima; Shinji Nagata; Tadao Asami; Yoshihito Suzuki, Last, Insect galls are abnormal plant tissues induced by galling insects. The galls are used for food and habitation, and the phytohormone auxin, produced by the insects, may be involved in their formation. We found that the silkworm, a non-galling insect, also produces an active form of auxin, indole-3-acetic acid (IAA), by de nova synthesis from tryptophan (Trp). A detailed metabolic analysis of IAA using IAA synthetic enzymes from silkworms indicated an IAA biosynthetic pathway composed of a three-step conversion: Trp -> indole-3-acetaldoxime -> indole-3-acetaldehyde (IAAld) -> IAA, of which the first step is limiting IAA production. This pathway was shown to also operate in gall-inducing sawfiy. Screening of a chemical library identified two compounds that showed strong inhibitory activities on the conversion step IAAld IAA. The inhibitors can be efficiently used to demonstrate the importance of insect-synthesized auxin in gall formation in the future. (C) 2014 Elsevier Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, Oct. 2014, [Reviewed] - Phytohormones in Japanese Mugwort Gall Induction by a Gall-Inducing Gall Midge
Yuichiro Tanaka; Koichi Okada; Tadao Asami; Yoshihito Suzuki, Last, A variety of insect species induce galls on host plants. Liquid chromatographic/tandem mass spectrometric analyses showed that a gall midge (Rhopalomyia yomogicola) that induces galls on Artemisia princeps contained high levels of indole-3-acetic acid and cytokinins. The gall midge larvae also synthesized indole-3-acetic acid from tryptophan. Close observation of gall tissue sections indicated that the larval chamber was surrounded by layers of cells having secondary cell walls with extensive lignin deposition, except for the part of the gall that constituted the feeding nutritive tissue which was composed of small cells negatively stained for lignin. The differences between these two types of tissue were confirmed by an expression analysis of the genes involved in the synthesis of the Secondary cell wall. Phytohormones may have functioned in maintaining the feeding part of the gall as fresh nutritive tissue. Together with the results in our previous study, those presented here suggest the importance of phytohormones in gall induction., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2013, [Reviewed] - Phytohormones and willow gall induction by a gall-inducing sawfly
Hiroki Yamaguchi; Hiroki Tanaka; Morifumi Hasegawa; Makoto Tokuda; Tadao Asami; Yoshihito Suzuki, Last, A variety of insect species induce galls on host plants. Several studies have implicated phytohormones in insect-induced gall formation. However, it has not been determined whether insects can synthesize phytohormones. It has also never been established that phytohormones function in gall tissues. Liquid chromatography and tandem mass spectrometry (LC/MS/MS) were used to analyse concentrations of endogenous cytokinins and the active auxin IAA in the gall-inducing sawfly (Pontania sp.) and its host plant, Salix japonica. Feeding experiments demonstrated the ability of sawfly larvae to synthesize IAA from tryptophan. Gene expression analysis was used to characterize hormonal signalling in galls. Sawfly larvae contain high concentrations of IAA and t-zeatin, and produce IAA from tryptophan. The glands of adult sawflies, the contents of which are injected into leaves upon oviposition and are involved in the initial stages of gall formation, contain an extraordinarily high concentration of t-zeatin riboside. Transcript levels of some auxin- and cytokinin-responsive genes are significantly higher in gall tissue than in leaves. The abnormally high concentration of t-zeatin riboside in the glands strongly suggests that the sawfly can synthesize cytokinins as well as IAA. Gene expression profiles indicate high levels of auxin and cytokinin activities in growing galls., WILEY-BLACKWELL
NEW PHYTOLOGIST, Oct. 2012, [Reviewed] - Trans-Repression of Gene Activity Upstream of T-DNA Tagged RLK902 Links Arabidopsis Root Growth Inhibition and Downy Mildew Resistance
Colette A. ten Hove; Mark de Jong; Dmitry Lapin; Annemiek Andel; Gabino F. Sanchez-Perez; Yoshiaki Tarutani; Yoshihito Suzuki; Renze Heidstra; Guido van den Ackerveken, Receptor-like kinases (RLKs) constitute a large family of signal perception molecules in Arabidopsis. The largest group of RLKs is the leucine-rich repeat (LRR) class that has been described to function in development and defense. Of these, CLAVATA1 (CLV1) and ERECTA (ER) receptors function in maintaining shoot meristem homeostasis and organ growth, but LRR RLKs with similar function in the root remain unknown. For the interaction of Arabidopsis with the oomycete pathogen Hyaloperonospora arabidopsidis the involvement of LRR RLKs has not been demonstrated. A set of homozygous T-DNA insertion lines mutated in LRR RLKs was investigated to assess the potential role of these receptors in root meristem maintenance and compatibility. One mutant line, rlk902, was discovered that showed both reduced root growth and resistance to downy mildew in a recessive manner. The phenotypes of this mutated line could not be rescued by complementation, but are nevertheless linked to the T-DNA insertion. Microarray studies showed that gene expression spanning a region of approximately 84 kb upstream of the mutated gene was downregulated. The results suggest T-DNA mediated trans-repression of multiple genes upstream of the RLK902 locus links both phenotypes., PUBLIC LIBRARY SCIENCE
PLOS ONE, Apr. 2011, [Reviewed] - Feedback-Regulation of Strigolactone Biosynthetic Genes and Strigolactone-Regulated Genes in Arabidopsis
Kiyoshi Mashiguchi; Eriko Sasaki; Yukihisa Shimada; Miyu Nagae; Kotomi Ueno; Takeshi Nakano; Koichi Yoneyama; Yoshihito Suzuki; Tadao Asami, Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2009, [Reviewed] - Genome-Wide Identification, Structure and Expression Studies, and Mutant Collection of 22 Early Nodulin-Like Protein Genes in Arabidopsis
Kiyoshi Mashiguchi; Tadao Asami; Yoshihito Suzuki, Last, Early nodulin-like proteins (ENODLs) are chimeric arabinogalactan proteins (AGPs) related to the phytocyanin family. Although they show similarities with other phytocyanins, they lack amino acid residues for copper binding. Despite the existence of other phytocyanins, information about the function of ENODLs in plants is largely lacking. In this study, we characterized ENODL genes consisting of 22 members in Arabidopsis thaliana. Structure prediction indicated that most ENODLs are glycosylphosphatidylinositol-anchored chimeric AGPs. Expression analysis by real-time reverse transcription polymerase chain reaction indicated that most ENODL genes showed spatially specific expression, mainly in the flower organs. Furthermore, we obtained and analyzed 26 homozygous T-DNA insertion lines of 15 ENODL genes, but novel biological roles were not uncovered, probably due to functional redundancy. The detailed phylogenetic and expression analyses and characterization of the available insertion lines in this study might facilitate future studies to elucidate the biological roles of ENODLs., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2009, [Reviewed] - Anti-metatype peptides, a molecular tool with high sensitivity and specificity to monitor small ligands
J. Inaba; S. Nakamura; K. Shimizu; T. Asami and Y. Suzuki, Corresponding
Anal. Biochem., 10 Feb. 2009, [Reviewed] - A new gibberellin detection system in living cells based on antibody V-H/V-L interaction
Younggiu Lee; Tadao Asami; Isomaro Yamaguchi; Hiroshi Ueda; Yoshihito Suzuki, Last, As a new detection method of bioactive gibberellin A(4) (GA(4)) in living cells, a combined system of GA(4)-dependent interaction Of V-H and V-L composed of a variable region fragment (Fv) of anti-GA(4) antibodies and protein-fragment complementation assay (PCA) was developed. First, when V-H and V-L were displayed in proximity on a phage, they could constitute a functional Fv. Thereafter, V-H and V-L were shown to interact with each other in a GA(4)-dependent manner. We then applied this interaction to PCA using GFP as a reporter. V-H fused to the C-terminal half of GFP and V-L fused to the N-terminal half of GFP were simultaneously expressed in Escherichia coli. The E. coli in which these fusion proteins were inductively produced in the presence of GA(4) showed clear GFP fluorescence, while those in the absence of GA(4) showed only scarce GFP fluorescence, demonstrating the feasibility of this system to detect GA(4) in living Organisms. (C) 2008 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2008, [Reviewed] - Immunomodulation of gibberellin biosynthesis using an anti-precursor gibberellin antibody confers gibberellin-deficient phenotypes
Eriko Urakami; Isomaro Yamaguchi; Tadao Asami; Udo Conrad; Yoshihito Suzuki, Last, Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. We have recently succeeded in obtaining gibberellin (GA)-deficient phenotypes in Arabidopsis thaliana by using anti-bioactive GA antibodies. In this study, a single-chain antibody (scFv) against GA(24), a precursor GA, was utilized to repress the biosynthesis of bioactive gibberellins. Stable accumulation of the scFv in endoplasmic reticulum (ER) was achieved by being produced as a fusion with GFP as well as KDEL ER-retention signal. The transgenic plants showed GFP fluorescence in the reticulate cortical ER network in epidermal cells. The GFP-scFv fusion produced in plants maintained its binding activity. The transgenic plants showed GA-deficient phenotypes, including reduced rosette leaf development, delayed flower induction and reduced stem elongation of the main culm, especially in the early stage of inflorescence growth. Contrarily, stem elongation of the main culm at a later stage, or that of lateral shoots was much less affected by scFv production. These phenotypes were different from anti-bioactive GA scFv-producing lines, whose stem elongation was continuously repressed throughout the inflorescence development. The GA-deficient phenotypes were recovered by treatment with GA(24) and bioactive GA(4), the latter being more effective. The transgenic lines contained conspicuously higher endogenous GA(24) and clearly less GA(4) than wild-type plants. The expression of GA 20-oxidase and GA 3-oxidase genes, which are feedback-regulated by GA signaling, were up-regulated in those plants. These results demonstrate that the scFv trapped GA(24) in ER and inhibited metabolism of GA(24) to bioactive GA(4)., SPRINGER
PLANTA, Oct. 2008, [Reviewed] - Immunomodulation of bioactive gibberellin confers gibberellin-deficient phenotypes in plants
Yoshihito Suzuki; Toru Mizuno; Eriko Urakami; Isomaro Yamaguchi; Tadao Asami, Lead, Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. This method was applied to plants to repress the function of gibberellins (GAs), a class of phytohormones responsible for plant elongation, by anti-bioactive GA antibodies. Two different antibodies were produced in Arabidopsis as single-chain variable fragment (scFv) fused to green fluorescent protein (GFP) with four different subcellular localizations: endoplasmic reticulum (ER), cytosol, apoplastic space or the outer surface of the plasma membrane. When targeting scFv-GFP to ER, plants showed the highest accumulation of scFv-GFP, with binding activity, strong GFP fluorescence in ER-derived compartments and mild but clear GA-deficient phenotypes, including a smaller leaf size, delayed bolting, shorter inflorescence length and decreased germination. Plants expressing scFv-GFP in ER responded to exogenous GA, and contained 15-40 times greater endogenous GA, than wild-type plants. They also showed increased gene expression for GA3ox1, GA20ox1 and GA20ox2, but decreased expression for GA2ox1, which are feedback and feedforward regulated by GA signalling, respectively. These results suggest that the level of free functional GA(4) decreased when trapped in the ER with scFv to the extent that mild GA-deficient phenotypes were created. A dramatic increase in the total sum of GA4 (free plus scFv-GFP bound) was detected as a result of the up-regulation of GA biosynthesis (feedback regulated), and a decrease in GA, catabolism as a result of protection by scFv-GFP binding. This study demonstrates that the use of immunomodulation to inhibit the action of bioactive GAs is an effective method of creating GA-deficient plants., BLACKWELL PUBLISHING
PLANT BIOTECHNOLOGY JOURNAL, May 2008, [Reviewed] - Defense-related signaling by interaction of arabinogalactan proteins and beta-glucosyl Yariv reagent inhibits gibberellin signaling in barley aleurone cells
Kiyoshi Mashiguchi; Eriko Urakami; Morifumi Hasegawa; Kazutsuka Sanmiya; Ichiro Matsumoto; Isomaro Yamaguchi; Tadao Asami; Yoshihito Suzuki, Last, Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at the plasma membrane and in extracellular spaces. A synthetic chemical, beta-glucosyl Yariv reagent (beta-GlcY), binds specifically to AGPs. We previously reported that gibberellin signaling is specifically inhibited by beta-GlcY treatment in barley aleurone protoplasts. In the present study, we found that beta-GlcY also inhibited gibberellin-induced programmed cell death (PCD) in aleurone cells. We examined the universality and specificity of the inhibitory effect of beta-GlcY on gibberellin signaling using microarray analysis and found that beta-GlcY was largely effective in repressing gibberellin-induced gene expression. In addition, >100 genes were up-regulated by beta-GlcY in a gibberellin-independent manner, and many of these were categorized as defense-related genes. Defense signaling triggered by several defense system inducers such as jasmonic acid and a chitin elicitor could inhibit gibberellin-inducible events such as alpha-amylase secretion, PCD and expression of some gibberellin-inducible genes in aleurone cells. Furthermore, beta-GlcY repressed the gibberellin-inducible Ca(2+)-ATPase gene which is important for gibberellin-dependent gene expression, and induced known repressors of gibberellin signaling, two WRKY genes and a NAK kinase gene. These effects of beta-GlcY were also phenocopied by the chitin elicitor and/or jasmonic acid. These results indicate that gibberellin signaling is under the regulation of defense-related signaling in aleurone cells. It is also probable that AGPs are involved in the perception of stimuli causing defense responses., OXFORD UNIV PRESS
PLANT AND CELL PHYSIOLOGY, Feb. 2008, [Reviewed] - 28. Gene expression pattern and substrate specificity of a novel rice gibberellin 2-oxidase, OsGA2ox7
Nakamura Hidemitsu; Park Seung-Hyun; Tagiri Akemi; Okada Keiko; Hakata Makoto; Toki Seiichi; Nakajima Masatoshi; Suzuki Yoshihito; Asami Tadao; Ichikawa Hiroaki, Concentrations of bioactive gibberellins (GAs) are controlled by the balance between their synthesis and deactivation. Therefore, it is very important to know precise expression patter ns and substrate specificity of individual GA-synthesizing and -deactivating enzymes to understand the mechanisms regulating GA levels. Out of over 12,000 rice FOX (F__-ull-length cDNA O__-ver-eX__-presser) lines, we have found the ectopic over-expression of AK101758 gene conferred super-dwarf phenotype to rice plants. The AK101758 protein was homologous to six previously known rice GA 2-oxidases (GA2oxs). A phylogenetic analysis showed AK101758 was grouped into GA2oxs that hydroxylate C19-gibberellins. Accordingly, we designated the AK101758 gene as OsGA2ox7. In situ hybridization analyses showed that OsGA2ox7 was expressed at the base of shoot apical meristems just like OsGA2ox1. Furthermore, OsGA2ox7 transcripts were observed in glumous flower primordia, indicating that the deactivation of GAs is important for the differentiation of glumous flowers. The levels of endogenous bioactive GAs, GA_1 and GA_4, and that of an immediate precursor of GA_1, GA_<20>, were reduced in the super-dwarf OsGA2ox7-FOX rice. Interestingly, the level of GA_<19> was also decreased in the super-dwarf rice, indicating that OsGA2ox7 hydroxylates upstream intermediates of GA synthesis including C20-gibberellins. OsGA2ox7 protein produced in E. coli showed GA2ox activity. The substrate specificity is currently under examination., The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 2008 - The BIG gene is involved in regulation of sulfur deficiency-responsive genes in Arabidopsis thaliana
Ichiro Kasajima; Naoko Ohkama-Ohtsu; Yoko Ide; Hiroaki Hayashi; Tadakatsu Yoneyama; Yoshihito Suzuki; Satoshi Naito; Toru Fujiwara, The Arabidopsis thaliana mutants altered sulfur response 1-1 (asr1-1) and asr1-2 were isolated using the green fluorescent protein gene (GFP), as a marker, driven by a sulfur deficiency-responsive promoter containing the beta(SR) fragment, which is responsible for the induction of gene expression under sulfur deficiency. In the asr1 mutants, the expression of three sulfur deficiency-responsive genes beta(SR)-driven GFP, sulfate transporter 2;2 (SULTR2;2) and adenosine-5'-phosphosulfate reductase 1 (APR1) were induced in medium containing a normal sulfate concentration. The ASR1 locus was mapped to a 53-kb region on the upper arm of chromosome III; this is also the region of the BIG gene, which encodes a calossin-like protein necessary for the polar transport of auxin. The morphology of the asr1 mutants, i.e. reduced leaf size and inflorescence elongation, resembled that of big mutants. Using nucleotide sequence analysis of the BIG gene, we identified independent nonsense mutations in asr1-1 and asr1-2. To confirm that ASR1 was BIG, we established lines of transgenic A. thaliana carrying a transfer DNA (T-DNA) insertion in the BIG gene. In these T-DNA insertion mutants, mRNA levels of beta(SR)-driven GFP and APR1 were upregulated in normal sulfate medium. The F-1 plants from crosses between asr1-1 and T-DNA insertion lines exhibited reduced leaf size and inflorescence length, indicating that ASR1 was indeed BIG. Taken together, the present results established that BIG is involved in the regulation of beta(SR)-driven GFP and APR1 mRNA level gene expression. Indole-3-acetic acid also upregulated beta(SR)-driven GFP and APR1 together with SULTR2;2 mRNA level, suggesting that the big effect on beta(SR)-driven GFP and APR1 is a pleiotropic aspect of the BIG gene., BLACKWELL PUBLISHING
PHYSIOLOGIA PLANTARUM, Feb. 2007, [Reviewed] - Sequential regulation of gibberellin, brassinosteroid, and jasmonic acid biosynthesis occurs in rice coleoptiles to control the transcript levels of anti-microbial Thionin genes
Yukihiro Kitanaga; Cui Jian; Morifumi Hasegawa; Junshi Yazaki; Naoki Kishimoto; Shoshi Kikuchi; Hidemitsu Nakamura; Hiroaki Ichikawa; Tadao Asami; Shigeo Yoshida; Isomaro Yamaguchi; Yoshihito Suzuki, Last, Transcripts of thionin genes encoding antimicrobial peptides were present at a high level in rice coleoptiles just after germination, and decreased to an undetectable level after about 3 d, but this decline was suppressed by co-treatment with gibberellic acid (GA3) and brassinolide (BL). The temporal expression patterns of key enzyme genes for the biosyntheses of gibberellins (GAs) and brassinosteroids (BRs) were correlated with the fluctuation of thionin mRNAs. Jasmonic acid (JA) replaced the effect of GA3 and BL, and its change in endogenous level was parallel to that of the thionin genes. These results strongly suggest that thionin gene expression was positively regulated by JA, whose endogenous level was synergistically regulated by GAs and BRs. In contrast, thionin gene expression in etiolated seedlings remained high while the endogenous level of JA was low, suggesting the presence of another signaling pathway in the dark to maintain the thionin level., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Oct. 2006, [Reviewed] - Identification of a peptide mimic of bioactive gibberellins with affinity to GA 2-oxidase
Haruko Sekimoto; Seung-Hyun Park; Masatoshi Nakajima; Isomaro Yamaguchi; Yoshihito Suzuki, Last, Previously we reported the first example of peptide mimics of a small hydrophobic molecule, a phytohormone gibberellin. The second peptide mimic of gibberellin has been identified from random peptide libraries by its affinity to a type of catalyzing enzyme of gibberellins, which specifically recognizes bioactive gibberellins. These results suggest that even hydrophobic compounds can be mimicked by peptides., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2006, [Reviewed] - AtFLA11, a fasciclin-like arabinogalactan-protein, specifically localized in screlenchyma cells
S Ito; Y Suzuki; K Miyamoto; J Ueda; Yamaguchi, I, Corresponding, An arabinogalactan-protein macroarray of all 48 Arabidopsis arabinogalactan-protein genes was prepared as a handy detection system for arabinogalactan-protein gene expression. The major transcript in inflorescence stems was identified as AtFLA11. AtFLA11 is categorized as a fasciclin-like arabinogalactan-protein that possesses a fasciclin domain with a cell adhesion function in animal cells. AtFLA11 was specifically expressed in the sclerenchyma cells of infloreseence stems and siliques, which are characterized by their thick secondary cell walls, and was confirmed by immunostaining at the protein level. The fluctuation of AtFLA11 transcripts during the maturation process of sclerenchyma cells suggests its role in the formation of the secondary cell wall., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Oct. 2005, [Reviewed] - Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand
T Murata; H Hemmi; S Nakamura; K Shimizu; Y Suzuki; Yamaguchi, I, Corresponding, Using NMR spectroscopy and simulated annealing calculations, we determined the solution structure of the disulfide-linked cyclized decapeptide ACLPWSDGPC (SD), which is bound to an anti-(gibberellin A(4)) mAb 4-B8(8)/E9 and was found to be the first peptidyl mimotope for a hydrophobic ligand. The resulting structure of the peptide showed a beta-turn-like conformation in residues three to seven and the region converges well (average rmsd 0.54 angstrom). The binding activity and the epitopes of the peptide to the antibody were assessed using saturation transfer difference (STD)-NMR experiments. We also conducted docking simulations between the peptide and the mAb to determine how the peptide is bound to the mAb. Resonances around the beta-turn-like conformation of peptide SD (residues 3-5) showed strong STD enhancement, which agreed well with results from docking simulation between peptide SD and the mAb. Together with the commonality of amino acid residues of the mAb involved in interactions with gibberellin A(4) (GA(4)) and peptide SD, we concluded that peptide SD is bound to the antigen-binding site of mAb 4-B8(8)/E9 as a GA(4) mimic, confirming evidence for the existence of peptide mimics even for hydrophobic ligands., BLACKWELL PUBLISHING
FEBS JOURNAL, Oct. 2005, [Reviewed] - Secretion of bacterial xenobiotic-degrading enzymes from transgenic plants by an apoplastic expressional system: An applicability for phytoremediation
E Uchida; T Ouchi; Y Suzuki; T Yoshida; H Habe; Yamaguchi, I; T Omori; H Nojiri, In search of an effective method for phytoremediation of wastewater contaminated with organic compounds, we investigated the application of an apoplastic expressional system that secretes useful bacterial enzymes from transgenic plants into hydroponic media through the addition of a targeting signal. We constructed transgenic Arabidopsis expressing the aromatic-cleaving extradiol dioxygenase (DbfB), which degrades 2,3-dihydroxybiphenyl (2,3-DHB), and transgenic tobacco expressing haloalkane dehalogenase (OhaA), which catalyzes hydrolytic dechlorination of 1-chlorobutane (1-CB). Although crude leaf extracts of transgenic plants expressing cytoplasm-targeted degradative enzymes showed higher activity than did those from transgenic plants expressing apoplast-targeted enzymes, the hydroponic media of the latter showed 23.2 times (DbfB) and 76.4 times (DhaA) higher activity than plants containing the cytoplasm-targeted enzymes. Addition of crystalline 2,3DHB to 100 mL of the hydroponic medium of transgenic or wild-type seedlings revealed that only medium from the transgenic Arabidopsis expressing apoplast-targeted DbfB showed rapid ring cleavage of 2,3-DHB. Transgenic tobacco expressing apoplast-targeted DhaA also resulted in the accumulation of the dehalogenation product 1-butanol in the hydroponic medium and showed a higher tolerance to 1-CB than wild-type or transgenic plants expressing cytoplasm-targeted DhaA. These results demonstrate the usefulness of the apoplastic expression of bacterial recombinant proteins in phytoremediation., AMER CHEMICAL SOC
ENVIRONMENTAL SCIENCE & TECHNOLOGY, Oct. 2005, [Reviewed] - Systemic effect of a brassinosteroid on root nodule formation in soybean as revealed by the application of brassinolide and brassinazole
J Terakado; S Fujihara; S Goto; R Kuratani; Y Suzuki; S Yoshida; T Yoneyama, Leguminous plants form nitrogen-fixing root nodules and the number of nodules is controlled by a self-regulating mechanism called autoregulation. However, signaling substances involved in nodule regulation have not been identified. In the present study, we used brassinolide, a most effective molecular species of plant hormone brassinosteroids, and brassinazole, an effective inhibitor of brassinosteroid biosynthesis to determine whether brassinolide played a role in systemic regulation of nodule formation in wild type soybean and its super-nodulating mutant. Foliar application or direct injection of brassinolide into the root base inhibited nodule formation and root development in the super-nodulating mutant (En6500), but not in the parent line (cv. Enrei). The internodes in the plants subjected to foliar application were significantly longer than those in the untreated plants. In contrast, the application of brassinazole on mature leaves or into the culture media resulted in the increase of the nodule number in Enrei. These treatments also inhibited internodal growth in Enrei. The results indicate that brassinosteroids may regulate the nodule number in soybean plants. The function of brassinosteroids for the systemic regulation of nodule formation was examined., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
SOIL SCIENCE AND PLANT NUTRITION, Jun. 2005, [Reviewed] - Preparation of functional single-chain antibodies against bioactive gibberellins by utilizing randomly mutagenized phage-display libraries
Y Suzuki; S Ito; K Otsuka; E Iwasawa; M Nakajima; Yamaguchi, I, Lead, Screening randomly mutagenized proteins displayed on a phage surface by biopanning is a powerful strategy to obtain evolved clones with improved properties such as higher stability and functionality. We utilized this method to overcome the problem that functional single-chain antibodies against active gibberellins, a class of plant hormones, can not be prepared by some of the conventional methods. Single-chain antibody libraries with random mutations were constructed from two independent anti-bioactive gibberellin monoclonal antibody lines in a phagemid vector, so that the mutagenized scFvs were expressed in a phage-displayed form upon helper phage infection. From both libraries, scFv clones with binding activity to GA(4) were successfully obtained by successive rounds of biopanning against BSA-GA(4), the original immunogen. The results are highly suggestive that this approach might be a general solution when a single-chain antibody does not show binding activity. We found further that a ribosomal frameshift to complement a nonsense mutation frequently occurred in an amber suppressor strain of E. coli TG1, resulting in the display of a functional antibody, while such a nonsense mutant failed to produce a soluble antibody in a non-amber suppressor strain. This result explains at least partly why single-chain antibodies are sometimes functional only in a phage-displayed form, not in a soluble form., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Mar. 2005, [Reviewed] - Expression of a single-chain antibody against GA(24/19) in vascular tissues induces dwarf phenotype for rice plants
Y Tanaka; A Fukuda; S Nagai; T Fujiwara; Y Suzuki; Yamaguchi, I; T Yoneyama; H Hayashi, Corresponding, GA(1) and GA(4) are active gibberellins (CrAs) in rice plants, which are synthesized from GA(19) and GA(24), respectively. However, the plant tissues where active GAs biosynthesis occurs and the route of their transport have not been fully elucidated. To study the importance of vascular tissues for the function of GAs, cDNA encoding anti-GA(24/19) single-chain variable fragment (scFv) was expressed under the control of the thioredoxin h promoter, which is active in companion cells of phloem tissues. The transgenic rice plants showed a dwarf phenotype with increased levels of GA(19). This indicated that the expression of the anti-GA(24/19) scFv antibody in companion cells changed the synthesis and/or translocation of GAs in rice plants effectively, suggesting that the vascular tissues play an important role in the function of GAs in rice plants., JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
SOIL SCIENCE AND PLANT NUTRITION, Dec. 2004, [Reviewed] - Identification of three clones which commonly interact with the kinase domains of highly homologous two receptor-like kinases, RLK902 and RKL1
Y Tarutani; A Sasaki; M Yasuda; H Nakashita; S Yoshida; Yamaguchi, I; Y Suzuki, Last, We have previously reported the characterization of highly homologous two leucine-rich repeat (LRR)-receptor-like kinase (RLK) genes, RLK902 and RKL1, which showed 75% identity at the amino acid sequence level. To investigate the RLK902 and RKL1 mediated signal transduction pathways, we performed yeast two-hybrid screening using the kinase domains of RLK902 and RKL1 as baits. Three clones, Y-1, 2 and 3, were found to interact commonly with the kinase domain of RLK902 and RKL1 and not to interact with the kinase domain of BRIT, a member of LRR-RLKs. This result suggests that RLK902 and RKL1 may have common biochemical functions, especially in their downstream signal transduction. Furthermore, the detail analysis of their responsiveness to various conditions suggests their involvement in such stress conditions as mechanical wounding, treatment with salicylic acid, and pathogen infection., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Dec. 2004, [Reviewed] - Isolation and identification of glycosylphosphatidylinositol-anchored arabinogalactan proteins and novel beta-glucosyl Yariv-reactive proteins from seeds of rice (Oryza sativa)
K Mashiguchi; Yamaguchi, I; Y Suzuki, Last, Arabinogalactan proteins (AGPs) are highly glycosylated extracellular glycoproteins playing important roles in plant growth and development. We have previously reported the possibility that AGPs are involved in the induction of alpha-amylase by gibberellin (GA) in barley aleurone layers by using the beta-glucosyl Yariv reagent (beta-GlcY), which has been presumed to specifically bind AGPs. In this present study, we isolated beta-GlcY-reactive proteins from rice bran rich in aleurone cells. The N-terminal sequences of classical AGP and AG peptides were determined from hydrophilic fractions obtained by reversed phase HPLC. Interestingly, a novel non-specific lipid transfer protein-like protein (OsLTPL1) and a novel early nodulin-like protein (OsENODL1) were also identified in the more hydrophobic fractions from HPLC as beta-GlcY-reactive proteins. Expression analysis of the genes coding for these proteins was performed. While classical AGP, AG peptides and OsLTPL1 were expressed in various parts of rice, OsENODL1 showed temporally and spatially specific expression in the aleurone layers. This new beta-GlcY-reactive protein is a promising candidate for the extracellular signaling factors of GA action in cereal seeds. Furthermore, the possibility that proteins with the AG glycomodule might react with beta-GlcY may broaden the definition of AGPs., OXFORD UNIV PRESS
PLANT AND CELL PHYSIOLOGY, Dec. 2004, [Reviewed] - Identification,of peptidyl mimics of bioactive gibberellin recognized by an antibody
H Sekimoto; Y Suzuki; Yamaguchi, I, Corresponding, We screened a phage display peptide library for peptidyl mimotopes of gibberellin against anti-bioactive gibberellin antibody. The peptides obtained were grouped into two homologous sequences and their binding to the antibody was put in competition with free GA(4) but not with GA(4) methylester, suggesting that the peptides behave as mimics of GA(4). As an application, the phage display peptide was shown to work as a tracer for enzyme-linked immunosorbent assay (ELISA) analysis of GA(4)., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2004, [Reviewed] - Molecular characterization of two highly homologous receptor-like kinase genes, RLK902 and RKL1, in Arabidopsis thaliana
Y Tarutani; T Morimoto; A Sasaki; M Yasuda; H Nakashita; S Yoshida; Yamaguchi, I; Y Suzuki, Last, Receptor-like kinases (RLKs) constitute a large family of signal perception molecules. We characterized two highly homologous RLK genes, RLK902 and RKL1, in Arabidopsis. RLK902 and RKL1 showed a 75% amino acid sequence identity over their entire regions. In the RLK902 pro::GUS transgenic lines, GUS activity was strong in the root tips, lateral root primordia, stipules, and floral organ abscission zones, while the RKL1 promoter activity was dominant in the stomata cells, hydathodes and trichomes of young rosette leaves, and floral organ abscission zones. Neither the rlk902 mutant line, rkl1 mutant line nor rlk902/rkl1 double-knockout mutant line showed any significant phenotypes under normal growth conditions. These results suggest that RLK902 and RKL1 might mediate the signal transduction pathway in which at least one other complementary signaling pathway to these two RLKs might exist., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2004, [Reviewed] - 10. Expressional analysis of GA-responsive genes in tobacco BY-2 cells
Sakane Masayuki; Nakajima Masatoshi; Suzuki Yoshihito; Yamaguchi Isomaro, Tobacco cultured cell BY-2 is widely-used, because its synchronous culture and transformation systems have been established. The cells haven't been used for the research of GAs very often, however, because GA has no obvious effect on their growth. According to Amador et al. (2001), the expression level of a gene for GA-catabolism, GA 2-oxidase increased responding to GA, which suggested that the system required for GA-response still remained in BY-2 cells. Thus, we planed to elucidate the mechanism for the GA-response in the cells. We had obtained a GA 2-oxidase gene (NtGA2ox1) expressed in the cells by degenerate-PCR and RACEs, examined its expression patterns to applied GA_4 by using RNA-blottings, and confirmed its GA-repsonsiveness reported by Amador et al. Then we further studied its expression patterns in various culture conditions. Reaching the stationary phase of the cell-growth, the expression level of NtGA2ox1 increased. A halt of shaking elevated the expression level of NtGA2ox1, which made it difficult to see the response of the gene to GA. Incomplete aeration to the cells might have activated the expression of the gene. We examined the response of the gene to various GAs, and showed that some GAs with less or no biological activities showed lower effect on its expression than GA_4. We are going to evaluate the affinity of each GA to NtGA2ox1, using its recombinant., The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 2004 - A dwarf mutant strain of Pharbitis nil, Uzukobito (kobito), has defective brassinosteroid biosynthesis
Y Suzuki; K Saso; S Fujioka; S Yoshida; E Nitasaka; S Nagata; H Nagasawa; S Takatsuto; Yamaguchi, I, Lead, Japanese morning glory (Pharbitis nil) is a model plant characterized by a large stock of spontaneous mutants. The recessive mutant Uzukobito shows strong dwarfism with dark-green rugose leaves. The phenotype was rescued by the application of brassinolide, a bioactive brassinosteroid (BR), indicating that Uzukobito was a BR-deficient mutant. A detailed analysis of the endogenous BR levels in Uzukobito and its parental wild-type plant showed that Uzukobito had a lower level of BRs downstream of (24R)-24-methyl-5alpha-cholestan-3-one and (22S, 24R)-22-hydroxy-24-methyl-5alpha-cholestan-3-one than those in wild-type plants, while their immediate precursors (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one accumulated relatively more in Uzukobito. These results indicate that Uzukobito had a defect in the conversion of (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one to their 5alpha-reduced forms, which is catalyzed by de-etiolated2 (DET2) in Arabidopsis. The P. nil ortholog of the DET2 gene (PnDET2) was cloned and shown to have the greatest similarity to DET2 among all the putative genes in Arabidopsis. Uzukobito had one amino acid substitution from Glu(62) to Val(62) in the deduced amino acid sequence of PnDET2. Recombinant PnDET2 expressed in COS-7 cells was found to be a functional steroid 5alpha-reductase (S5alphaR) converting (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one, while PnDET2 with the mutation did not show any catalytic activity. This shows that a plant S5alphaR can convert an intrinsic substrate. All these results clearly demonstrate that the Uzukobito phenotype resulted from a mutation on PnDET2, and a morphological mutant has been characterized at the molecular level among a large stock of P. nil mutants., BLACKWELL PUBLISHING LTD
PLANT JOURNAL, Nov. 2003, [Reviewed] - Anti-herbicide single-chain antibody expression confers herbicide tolerance in transgenic plants
J Eto; Y Suzuki; H Ohkawa; Yamaguchi, I, Corresponding, An anti-chlorpropham single-chain variable-fragment (scFv) gene was introduced into Arabidopsis in a manner to express the antibody fragment in each of four different subcellular compartments. The accumulation of scFv in transgenic plants was detected by targeting the fragment in the endoplasmic reticulum or apoplastic space, or by expressing the fragment as a glycosylphosphatidylinositol-anchored protein, while no accumulation could be detected by targeting the fragment in the cytosol. Transgenic plants accumulating the scFv gene at a high level in the endoplasmic reticulum had enhanced tolerance to chlorpropham in comparison with the non-transformants. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies., ELSEVIER SCIENCE BV
FEBS LETTERS, Aug. 2003, [Reviewed] - CsAGP1, a gibberellin-responsive gene from cucumber hypocotyls, encodes a classical arabinogalactan protein and is involved in stem elongation.
Me Hea Park; Yoshihito Suzuki; Makiko Chono; J Paul Knox; Isomaro Yamaguchi, Corresponding, Fluorescence differential display was used to isolate the gibberellin (GA)-responsive gene, CsAGP1, from cucumber (Cucumis sativus) hypocotyls. A sequence analysis of CsAGP1 indicated that the gene putatively encodes a "classical" arabinogalactan protein (AGP) in cucumber. Transgenic tobacco (Nicotiana tabacum) plants overexpressing CsAGP1 under the control of the cauliflower mosaic virus 35S promoter produced a Y(betaGlc)(3)-reactive proteoglycan in addition to AGPs present in wild-type tobacco plants. Immuno-dot blotting of the product, using anti-AGP antibodies, showed that the CsAGP1 protein had the AGP epitopes common to AGP families. The transcription level of CsAGP1 in cucumber hypocotyls increased in response not only to GA but also to indole-3-acetic acid. Although CsAGP1 is expressed in most vegetative tissues of cucumber, including the shoot apices and roots, the GA treatment resulted in an increase in the mRNA level of CsAGP1 only in the upper part of the hypocotyls. Y(betaGlc)(3), which selectively binds AGPs, inhibited the hormone-promoted elongation of cucumber seedling hypocotyls. Transgenic plants ectopically expressing CsAGP1 showed a taller stature and earlier flowering than the wild-type plants. These observations suggest that CsAGP1 is involved in stem elongation.
Plant physiology, Mar. 2003, [Reviewed] - Effects of partial suppression of ribosomal protein s6 on organ formation in Arabidopsis thaliana
T Morimoto; Y Suzuki; Yamaguchi, I, Corresponding, An expression library of Arabidopsis thaliana cDNAs was randomly introduced into A. thaliana. The transformant pool was used to obtain a line, c105, with reduced apical dominance and irregular positioning of leaves and flowers. The inserted DNA was a 3'-fragment of the ribosomal protein S6 gene with antisense orientation. The transcriptional level of the ribosomal protein S6 was lower in c105 than in the wild-type plant. Introduction of the same fragment into the wild-type plant gave phenotypes similar to those of c105, so the phenotypes of c105 were due to the S6 antisense expression. The phenotypes suggest selectively reduced function of specific proteins rather than an overall decrease in protein function caused by defective ribosomal biogenesis., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2002, [Reviewed] - Cloning and characterization of the abscisic acid-specific glucosyltransferase gene from adzuki bean seedlings
ZJ Xu; M Nakajima; Y Suzuki; Yamaguchi, I, The glycosylated forms of abscisic acid (ABA) have been identified from many plant species and are known to be the forms of ABA-catabolism, although their (physiological) roles have not yet been elucidated. ABA-glucosyltransferase (-GTase) is thought to play a key role in the glycosylation of ABA. We isolated an ABA-inducible GTase gene from UDP-GTase homologs obtained from adzuki bean (Vigna angularis) seedlings. The deduced amino acid sequence (accession no. AB065190) showed 30% to 44% identity with the known UDP-GTase homologs. The recombinant protein with a glutathione S-transferase-tag was expressed in Escherichia coli and showed enzymatic activity in an ABA-specific manner. The enzymatic activity was detected over a wide pH range from 5.0 to 9.0, the optimum range being between pH 6.0 and 7.3, in a citrate and Tris-HCI buffer. The product from racemic ABA and UDP-D-glucose was identified to be ABA-GE by gas chromatography/mass spectrometry. The recombinant GTase (rAOG) converted 2-trans-(+)-ABA better than (+)-S-ABA and (-)-RABA. Although trans-cinnamic acid was slightly converted to its conjugate by the GTase, (-)-PA was not at all. The mRNA level was increased by ABA application or by water stress and wounding. We suggest that the gene encodes an ABA-specific GTase and that its expression is regulated by environmental stress., AMER SOC PLANT BIOLOGISTS
PLANT PHYSIOLOGY, Jul. 2002, [Reviewed] - A role for arabinogalactan proteins in gibberellin-induced alpha-amylase production in barley aleurone cells
Y Suzuki; M Kitagawa; JP Knox; Yamaguchi, I, Lead, Arabinogalactan proteins (AGPs) are plant proteoglycans that have been implicated in plant growth and development. The possible involvement of AGPs in the action of gibberellin (GA), a class of plant hormones, was examined by applying beta-glucosyl Yariv reagent (beta-Glc)(3)Y, a synthetic phenyl glycoside that interacts selectively with AGPs, to barley aleurone protoplasts. Gibberellin induces transcription and secretion of alpha-amylases in the protoplasts. Induction of alpha-amylase was clearly inhibited by (beta-Glc)(3)Y but not by (alpha-Gal)(3)Y, a negative control of the Yariv reagent that does not interact with AGPs. Transfection analysis, using an alpha-amylase promoter-GUS fusion gene in the protoplasts, indicated that the transcriptional activation of the alpha-amylase promoter was inhibited specifically by (beta-Glc)(3)Y. These observations are the first indication of an involvement of AGPs in a plant hormone function. The inhibitory effect of (beta-Glc)(3)Y was not observed when aleurone layers or half-seed grains were used. This result, together with the fact that protoplasts do not have cell walls, suggests that the AGPs that function in alpha-amylase induction reside at the plasma membrane. An aleurone-specific AGP was detected by reversed-phase HPLC, and supported the idea that an AGP may play an important role in aleurone-specific events. The possible mechanism of AGP function in gibberellin-induced alpha-amylase production is discussed., BLACKWELL PUBLISHING LTD
PLANT JOURNAL, Mar. 2002, [Reviewed] - Preparation of a functional single-chain antibody against chlorpropham
J Eto; Y Suzuki; H Ohkawa; Yamaguchi, I, Corresponding, PESTICIDE SCI SOC JAPAN
JOURNAL OF PESTICIDE SCIENCE, 2002, [Reviewed] - Expression pattern of the CsPK3 auxin-responsive protein kinase gene
M Chono; Y Suzuki; K Nemoto; H Yamane; N Murofushi; Yamaguchi, I, We have previously cloned a cDNA of a putative serine/threonine protein kinase gene named CsPK3 from cucumber, the mRNA level of which was up-regulated by auxin and down-regulated by light irradiation. To examine the CsPK3 gene expression in detail, we cloned a genomic DNA of CsPK3 gene and made transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) plants containing the fused CsPK3 promoter-beta -glucuronidase gene. The beta -glucuronidase expression was detected in the shoot apex, vascular tissues, and the outermost layer of cortex. The histological distribution of CsPK3 mRNA in cucumber seedlings was supported by in situ hybridization, where the positive signals were observed in similar tissues as those observed by beta -glucuronidase staining. The responsiveness of the CsPK3 gene to auxin and light was also confirmed for beta -glucuronidase activity. The pattern of beta -glucuronidase staining changed during the development of the tobacco seedlings. The results of our experiment showed that CsPK3 was expressed in a wide variety of tissues and cells in which the developmental and growth controls by auxin are suggested., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Mar. 2001, [Reviewed] - A novel transposon tagging element for obtaining gain-of-function mutants based on a self-stabilizing Ac derivative
Y Suzuki; S Uemura; Y Saito; O Murofushi; G Schmitz; K Theres; Yamaguchi, I, Lead, A novel tagging system AcREH, designed for obtaining gain-of-function mutations, was prepared on the basis of a self-stabilizing Ac transposon derivative. The transposable element, DsAT, was constructed in a way that it can activate transcription of neighboring genes by two 35S promoters and/or by four tandem repeats of the enhancer fragment of this promoter. DsAT revealed somatic excision in the first generation of the tobacco transformants. The element exhibited germinal excision to the next generation, as demonstrated by PCR and Southern hybridization analysis. In spite of the structure of the element, which may inhibit the expression of the transposase gene, the frequency of germinal excision was comparable to or higher than those so far reported, suggesting the applicability of the element for gene tagging., KLUWER ACADEMIC PUBL
PLANT MOLECULAR BIOLOGY, Jan. 2001, [Reviewed] - Pharbitis class-1 knotted-like homeobox gene, PKn3, shares similar characteristics to those of class-2 knotted-like genes
A Kobayashi; N Kiyosawa; Y Suzuki; N Murofushi; Yamaguchi, I, Corresponding, Three novel homeobox genes, PKn1-3 (Pharbitis knotted-like), were isolated from Japanese morning glory (Pharbitis nil Chois, strain Violet). A sequence analysis showed that these genes belong to the knotted class-1 gene family and that PKn3 has a relatively unique sequence. All PKn genes are expressed in shoot apices, stems and roots, but not in cotyledons. Transformed tobacco with PKn1 or PKn2 displayed leaf shrinkage and a dwarf phenotype, while the ectopic expression of PKn3 gave no altered phenotypes. In situ hybridization showed that PKn3 is up regulated in developing leaf primordia and that this expression becomes restricted in the basal region of young leaf blades, which is reminiscent of the expression pattern of the class-2 knotted gene, NTH23. These data suggest that these Pharbitis homeobox genes participate in the differentiation in shoots and suggest a unique function of PKn3 in developing leaves., SPRINGER-VERLAG
PLANT CELL REPORTS, Sep. 2000, [Reviewed] - Cloning and characterization of ABA-specific glucosyltransferase gene from adzuki bean
Xu Zheng-Jun; NAKAJIMA Masatoshi; SUZUKI Yoshihito; YAMAGUCHI Isomaro, The Janapese Society for Chemical Regulation of Plants
Chemical Regulation of Plants, 2000 - Cloning and characterization of ABA-specific glucosyltransferase gene from adzuki bean
Xu Zheng-Jun; Nakajima Masatoshi; Suzuki Yoshihito; Yamaguchi Isomaro, The occurrence of glucosylated ABA such as ABA-O-glucoside or ABA glucosyl ester in many plant species has been reported. Their physiological roles, however, remain unknown. Under the hypothesis that the glucosylation participates in the regulation of endogenous level of free ABA, we tried to isolated cDNAs which encode gucosyl transferases(GTase) catalizing the glucosylation of ABA. PCR was carried out using mRNA prepared from the ABA treated azuki epicotyls and the degenerated primers for the conserved region in many GTase homologs. To facilitate the selection of the target genes, we focused to screen the ABA-upregulated UDP-GTase genes. We detected one by northern hybridization analysis using the PCR products as probes. We cloned the full length cDNA from the library prepared from ABA treated azuki epicotyls. The putative amino acid sequence encoded by the cDNA showed 30-44% identity with known homologs. The GST-fused recombinant protein was expressed in E. coli and showed the activity glucosylating ABA when incubated with tritiated ABA and UDP-glucose. The study on the substrate specificity of the protein is under way., The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 2000 - Preparation and application of anti-idiotypic antibody against anti-gibberellin A(4) antibody
Y Suzuki; N Shimada; R Niwa; M Yokoi; M Nakajima; N Murofushi; Yamaguchi, I, Lead, A monoclonal anti-idiotypic antibody was raised against anti-gibberellin A(4) (GA(4)) antibody, which recognizes biologically active gibberellins such as GA(1) and GA(4) specifically, Amino acid sequences of variable regions of both anti-GA(4) and anti-idiotypic antibodies were analyzed. By using the property of the anti-idiotypic antibody to compete with GA(1/4) in binding to the anti-GA(4) antibody, we successfully applied the anti-idiotypic antibody to ELISA as a tracer for measuring GA(1/4). The single-chain Fv (scFv) gene of the anti-idiotypic antibody was constructed, and scFv expressed in E. coli showed binding activity to anti-GA(4) antibody. These results suggest the possible application of anti-idiotypic antibody as a handy and stable source of an enzymatic tracer for ELISA by production of fusion protein of the scFv and an appropriate enzyme., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 1999, [Reviewed] - Expression of a functional single-chain antibody against GA(24/19) in transgenic tobacco
N Shimada; Y Suzuki; M Nakajima; U Conrad; N Murofushi; Yamaguchi, I, Corresponding, An anti-gibberellin A(24/19) single-chain Fv gene was constructed from gamma and kappa genes cloned from a hybridoma cell line producing monoclonal antibody against gibberellin A(24/19), biosynthetic precursors of gibberellin A(4/1) which are biologically active per se. The single-chain Fv gene was introduced into tobacco plants after the binding activity of the single-chain Fv expressed in Escherichia coli was confirmed. When the single-chain Fv expression is targeted to endoplasmic reticulum, the plants could accumulate the single-chain Fv protein with the antigen binding activity up to 3.6% of the total soluble protein. On the other hand, when the expression is targeted to cytosol, accumulation of the single-chain Fv protein was not detected at all. The dwarf phenotype of the transgenic plants expressing the single-chain Fv protein, together with the preliminary analytical data indicating a decreased level of gibberellin A(1) in the dwarf transgenics, suggested that the single-chain Fv decreased the concentration of bioactive gibberellins by trapping and inhibiting the metabolism of gibberellin A(24) and/or A(19) to gibberellin A(4) and/or A(1)., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 1999, [Reviewed] - Construction and characterization of escherichia coli disruptants defective in the yaeM Gene
Naoki Shimada; Yoshihito Suzuki; Masatoshi Nakajima; Udo Conrad; Noboru Murofushi; Isomaro Yamaguchi, An anti-gibberellin A24/19single-chain Fv gene was constructed from γ and κ genes cloned from a hybridoma cell line producing monoclonal antibody against gibberellin A24/19, biosynthetic precursors of gibberellin A4/1which are biologically active per se. The single-chain Fv gene was introduced into tobacco plants after the binding activity of the single-chain Fv expressed in Escherichia coli was confirmed. When the single-chain Fv expression is targeted to endoplasmic reticulum, the plants could accumulate the single-chain Fv protein with the antigen binding activity up to 3.6% of the total soluble protein. On the other hand, when the expression is targeted to cytosol, accumulation of the single-chain Fv protein was not detected at all. The dwarf phenotype of the transgenic plants expressing the single-chain Fv protein, together with the preliminary analytical data indicating a decreased level of gibberellin A1in the dwarf transgenics, suggested that the single-chain Fv decreased the concentration of bioactive gibberellins by trapping and inhibiting the metabolism of gibberellin A24and/or A19to gibberellin A4and/or A1. © 1999, Taylor &
Francis Group, LLC. All rights reserved.
Bioscience, Biotechnology and Biochemistry, 01 Jan. 1999, [Reviewed] - 4 Isolation of gibberellin-inducible glucosyltransferases from Adzuki bean
Xu Zheng-Jun; Nakajima Masatoshi; Suzuki Yoshihito; Yamaguchi Isomaro, Gibberellin glycosides (Gas-G) have been identified from many plant species and considered to be a form of GA-catabolites, although their physiological role is not clear yet. We attempted to isolate a GA-specitically inducible glucosyltransferase genes with the speculation that a kind of GA-specitic glucosyltransferases exists and plays a key role in the glucosylation of Gas. We picked up 28 PCR fragments of UDP-glucosyltransferase homologs from the cDNA prepared from Adzuki bean seedlings. Northern hybridization experments showed [I] three of the homologs were GA-inducible, [ii] their mRNA expression levels induced by GA were different. Their putative amino acid sequence showed 30%-45% identity with the known UDP-glucosyltransferase homologs., The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 1999 - Isolation of gibberellin-inducible glucosyltransfer-ases from Adzuki bean.
Zheng-Jun Xu; NAKAJIMA Masatoshi; SUZUKI Yoshihito; YAMAGUCHI Isomaro, The Janapese Society for Chemical Regulation of Plants
Chemical Regulation of Plants, 1999 - D-43 Analysis of transgenic plants expressing anti-GA24 single-chain Fv
SHIMADA Naoki; SUZUKI Yoshihito; YOKOI Michinori; NAKAJIMA Masatoshi; MUROFUSHI Noboru; YAMAGUCHI Isomaru; Udo Conrad, We generated a scFv against GA19/24. These GAs are considered to be pool and/or translocating forms. The anti-GA24scFv expressed in E.coli showed binding-activity to its antigen. The anti-GA24 scFv gene was introduced into tobacco. The vectors were designed to target the scFv into either ER or cytosol, using the combination of signal peptide and ER-retention signal. Western blotting analysis showed the accumulation of the scFv in the plants designed to retain the scFv in ER. We will report further information such as binding-activity of the scFv in plants, GAs contents, phenotypes of the transgenic plants. No accumulation of the scFv was observed when it was targetted to cytosol., The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 1998 - 13 Introduction anti-GA_<24> single-chain FV gene into tobacco
Shimada Naoki; Suzuki Yoshihito; Yokoi Michinori; Nakajima Masatoshi; Yamaguchi Isomaro; Murofushi Noboru; Conrad Udo, The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 1997 - The dwarf-1 (d1) mutant of Zea mays blocks three steps in the gibberellin-biosynthetic pathway
CR Spray; M Kobayashi; Y Suzuki; BO Phinney; P Gaskin; J MacMillan, In plants, gibberellin (GA)-responding mutants have been used as tools to identify the genes that control specific steps in the GA biosynthetic pathway, They have also been used to determine which native GAs are active per se, i.e., further metabolism is not necessary for bioactivity. We present metabolic evidence that the D1 gene of maize (Zea mays L.) controls the three biosynthetic steps: GA(20) to GA(1), GA(20) to GA(5), and GA(5) to GA(3). We also present evidence that three gibberellins, GA(1), GA(5), and GA(3), have per se activity in stimulating shoot elongation in maize, The metabolic evidence comes from the injection of [17-C-13,H-3]GA(20) and [17-C-13,H-3]GA(5) into seedlings of d1 and controls (normal and d5), followed by isolation and identification of the C-13-labeled metabolites by full-scan GC-MS and Kovats retention index, For the controls, GA(20) was metabolized to GA(1), GA(3), and GA(5); GA(5) was metabolized to GA(3), For the d1 mutant, GA(20) was not metabolized to GA(1), GA(3), or to GA(5), and GA(5) was not metabolized to GA(3). The bioassay evidence is based on dosage response curves using d1 seedlings for assay. GA(1), GA(3), and GA(5) had similar bioactivities, and they were 10-times more active than GA(20)., NATL ACAD SCIENCES
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Sep. 1996, [Reviewed] - 11 Preparation of single-chain Fvs derived from aniti-GA-and aniti-idiotypic antibodies
Shimada Naoki; Suzuki Yoshihito; Uemura Satoko; Niwa Rimpei; Nakajima Masatoshi; Asami Tadao; Yamaguchi Isomaro; Murofushi Noboru, The Janapese Society for Chemical Regulation of Plants
The Janapese Society for Chemical Regulation of Plants, Abstract, 1996 - ENDOGENOUS GIBBERELLINS IN AEGINETIA-INDICA, A PARASITIC PLANT, AND ITS HOST, MISCANTHUS-SINENSIS
H SUWA; Y SUZUKI; YH ZHANG; N MUROFUSHI; Y TAKEUCHI, Corresponding, Endogenous gibberellins in a parasitic plant, Aeginetia indica L,, and its host, Miscanthus sinensis Andress (eulalia) were analyzed, Gibberellins of the early-non-hydroxylation pathway and their putative metabolites were identified as the major endogenous gibberellins from both types of A, indica parasitizing M, sinensis and parasitizing Oryza sativa L, (rice), Members of both the early-non- and early-13-hydroxylation pathways were detected in the host M, sinensis, Since the early-13-hydroxylation pathway has been reported to he the major pathway operating in vegetative tissues of O, sativa, these results suggest that A, indica can biosynthesize gibberellins independent of its hosts., TAYLOR & FRANCIS LTD
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 1995, [Reviewed] - METABOLISM AND TRANSLOCATION OF GIBBERELLINS IN SEEDLINGS OF PHARBITIS-NIL .1. EFFECT OF PHOTOPERIOD ON STEM ELONGATION AND ENDOGENOUS GIBBERELLINS IN COTYLEDONS AND THEIR PHLOEM EXUDATES
YY YANG; YAMAGUCHI, I; K TAKENOWADA; Y SUZUKI; N MUROFUSHI, Gibberellin A(1) (GA(1)), GA(19), and GA(20), in phloem exudates and cotyledons of seedlings of Pharbitis nil cv. Violet, grown under different photoperiodic conditions, were qualitatively and semi-quantitatively analyzed by a combination of high performance-liquid chromatography (HPLC) and radioimmunoassays (RIA). The levels of GA(19) and GA(20) were higher in cotyledons from plants grown under dark treatment (DT) conditons of 16 h-light/8 h-dark for 6 days followed by 8 h-light/16 h-dark for 3 days than in those grown under continuous light (CL) for 9 days. This relationship was also observed for the GAs in phloem exudates, although the levels were much lower than in the cotyledons. When GAs were applied to the cotyledons, elongation of the epicotyl was promoted more by GA(20) than by GA(1) or GA(19), especially under the CL treatment. The relative effect of GA(1) and GA(20) on the epicotyl elongation was reversed when these GAs were applied to epicotyls pre-treated with prohexadione, an inhibitor of 2-oxoglutarate-dependent dioxygenases., JAPANESE SOC PLANT PHYSIOLOGISTS
PLANT AND CELL PHYSIOLOGY, Mar. 1995, [Reviewed] - 3-EPIGIBBERELLIN A(1) - NATURAL OCCURRENCE IN PLANTS AND ARTIFACTUAL FORMATION FROM GIBBERELLIN A(1)
P GASKIN; J MACMILLAN; CR SPRAY; Y SUZUKI; BO PHINNEY, Whether 3-epigibberellin(3-epiGA(1)) is endogenous in plants or is formed from GA(1) as an artefact, can be determined by adding [C-13]GA(1) to the plant material as an internal reference and measuring the C-12: C-13 ratio of 3-epiGA(1) and GA(1), recovered from the plant extract. We use this method to show that 3-epiGA(1) is not a natural constituent of vegetative tissues of Zea mays (maize) but is endogenous in Lactuca sativa (lettuce)., PERGAMON-ELSEVIER SCIENCE LTD
PHYTOCHEMISTRY, Jan. 1995, [Reviewed] - Endogenous gibberellins in clover broomrape, a parasitic plant, and its host, clover: Dependency of the parasite on the host for gibberellin production
Yoshihito Suzuki; Noboru Murofushi; Yun-Hui Zhang; Yasutomo Takeuchi, Lead, Endogenous gibberellins were analyzed from a parasitic plant, clover broomrape (Orobanche minor Smith), and its host, clover (Trifolium repens L.). Members of both the early-13- and the early-non-hydroxylation pathways were identified from both the parasite and the host (GA12, GA24, GA9 GA4, GA44, GA19, GA20, and GA1 from clover broomrape
GA9, GA4, GA44, GA19, GA20, and GA1 from clover). Quantitative analyses showed that GA44 was present at high levels in both host and parasite. The similarity in the gibberellins suggests the possibility that the major gibberellins in clover broomrape are transported from clover. However gibberellins such as GA58, GA38, and notably GA47 which was identified from a plant for the first time were detected only from clover broomrape, suggesting that the parasite may have the ability to produce at least those gibberellins © 1994 Springer-Verlag New York Inc., Springer-Verlag
Journal of Plant Growth Regulation, Jun. 1994, [Reviewed] - METABOLISM AND BIOLOGICAL-ACTIVITY OF GIBBERELLIN A4 IN VEGETATIVE SHOOTS OF ZEA-MAYS, ORYZA-SATIVA, AND ARABIDOPSIS-THALIANA
M KOBAYASHI; P GASKIN; CR SPRAY; Y SUZUKI; BO PHINNEY; J MACMILLAN, [17-C-13,H-3]Gibberellin A4 (GA4) was injected into the shoots of tall (W23/L317), dwarf-1 (d1), and dwarf-5 (d5) Zea mays L. (maize); tall (cv Nipponbare), dwarf-x (dx), and dwarf-y (dy) Oryza sativa L. (rice); and tall (ecotype Landsberg erecta), ga4, and ga5 Arabidopsis thaliana (L.) Heynh. [C-13]GA4 and its metabolites were identified from the shoots by full-scan gas chromatography-mass spectrometry and Kovats retention indices. GA4 was metabolized to GA1 in all nine genotypes. GA4 was also metabolized in some of the genotypes to 3-epi-GA1, GA2, 2beta-OH-GA2, 3-epi-GA2, endo-GA4, 16alpha,17-H-2-16,17-(OH)2-GA4, GA34, endo-GA34, GA-58, 15-epi-GA63, GA71, and 16-epi-GA82. No evidence was found for the metabolism of GA4 to GA7 or of GA4 to GA3. The bioactivities of GA4 and GA1 were determined using the six dwarf mutants for assay. GA4 and GA1 had similar activities for the maize and rice mutants. For the Arabidopsis mutants, GA4 Was more active than GA1 at low dosages; GA4 was less active than GA1 at higher dosages., AMER SOC PLANT PHYSIOLOGISTS
PLANT PHYSIOLOGY, Jun. 1993, [Reviewed] - Endogenous gibberellins in parasitic plants
Y. Suzuki; Y-H. Zhang; H. Suwa; N. Murofushi; and Y. Takeuchi, Lead
Proc. JSPS-NUS Seminar on Physiol. Mol. Aspects of Plant Growth and Differetiation, 1993 - PROMOTION OF FLOWERING AND MORPHOLOGICAL ALTERATIONS IN ATROPA-BELLADONNA TRANSFORMED WITH A CAMV 35S-ROLC CHIMERIC GENE OF THE RI PLASMID
Y KURIOKA; Y SUZUKI; H KAMADA; H HARADA, Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacteriwn mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of die rolC gene., SPRINGER VERLAG
PLANT CELL REPORTS, Dec. 1992, [Reviewed] - METABOLISM OF ENT-KAURENE TO GIBBERELLIN A12-ALDEHYDE IN YOUNG SHOOTS OF NORMAL MAIZE
Y SUZUKI; H YAMANE; CR SPRAY; P GASKIN; J MACMILLAN; BO PHINNEY, Lead, Young shoots of normal maize (Zea mays L.) were used to determine both the stepwise metabolism of ent-kaurene to gibberellin A12-aldehyde and the endogenous presence of the members in this series. Each of the five steps in the sequence was established by feeds of 17-C-13,H-3-labeled kauranoids to cubes from the cortex of elongating internodes, to homogenates from the cortex of elongating internodes, and/or to homogenates from dark-grown seedlings. The C-13-metabolites were identified by Kovats retention indices (KRI) and full-scan capillary gas chromatography-mass spectrometry (GC-MS). Five substrates and the final product in this sequence were shown to be native by the isotopic dilution of 17-C-13,H-3-labeled substrates added as internal standards to extracts obtained from elongating internodes. Evidence for the isotopic dilution was obtained by KRI and full-scan capillary GC-MS. Thus, we document the presence in young maize shoots of the metabolic steps, ent-kaurene --> ent-kaurenol --> ent-kaurenal --> ent-kaurenoic acid --> ent-7-alpha-hydroxykaurenoic acid --> gibberellin A12-aldehyde., AMER SOC PLANT PHYSIOLOGISTS
PLANT PHYSIOLOGY, Feb. 1992, [Reviewed] - Physiological characteristic and secondary metabolite production in Atropa belladonna transformed with a rolC gene of Ri plasmid.
H.Kamada; Y.Kurioka; Y. Suzuki; and H. Harada
Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals, 1992 - GIBBERELLIN METABOLISM IN MAIZE - TISSUE-SPECIFICITY
BO PHINNEY; CR SPRAY; Y SUZUKI; P GASKIN, SPRINGER-VERLAG BERLIN
GIBBERELLINS, 1991 - IDENTIFICATION AND SEMI-QUANTIFICATION OF GIBBERELLINS FROM THE POLLEN AND ANTHERS OF ZEA-MAYS BY IMMUNOASSAY AND GC/MS
YAMAGUCHI, I; H NAKAZAWA; R NAKAGAWA; Y SUZUKI; S KUROGOCHI; N MUROFUSHI; N TAKAHASHI; EW WEILER, Radioimmunoassays and enzyme-linked immunosorbent assays for methyl esters of gibberellins A1, A3, A4, and A7 were established using an antiserum specific for GA1-Me. The antiserum was characterized by high titer and specificity for such C19-GAs with 3-beta-hydroxyl group as GA1, GA3, GA4 and GA7. Combination of this antiserum and HPLC enabled us to identify and quantify GA1 and GA4 from the pollen of Zea mays with a high degree of reliability. Similarly, identification and quantification of GA9 and GA20 were also made possible by use of an antiserum specific for GA20-Me. Combined use of immunoassays and GC/MS enabled us to identify nine GAs from the pollen and four from the anthers of Zea mays. The identification of non-13-hydroxylated GAs, such as GA4 and GA9, in addition to 13-hydroxylated GAs from the pollen and the anthers suggests that the early-non-hydroxylation pathway, as well as the early-13-hydroxylation pathway, operates in the male reproductive organs of Zea mays, and that the organ-specific biosynthesis and/or localization of GAs in Zea mays is similar to that in Oryza sativa., JAPANESE SOC PLANT PHYSIOLOGISTS
PLANT AND CELL PHYSIOLOGY, Dec. 1990, [Reviewed] - SYNTHESIS OF BENZYL 6-O-BETA-D-APIOFURANOSYL-BETA-D-GLUCOPYRANOSIDE, A METABOLITE OF BENZOIC-ACID IN LEMNA-PAUCICOSTATA
Y SUZUKI; YAMAGUCHI, I; N MUROFUSHI; N TAKAHASHI; F SUGAWARA; S YOSHIDA; T NUKADA; T OGAWA, Lead, JAPAN SOC BIOSCI BIOTECHN AGROCHEM
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, May 1988, [Reviewed] - IDENTIFICATION OF PHENYLGLYOXAL AS A FLOWER-INDUCING SUBSTANCE OF LEMNA FROM PHARBITIS-PURPUREA
Y SUZUKI; YAMAGUCHI, I; N MUROFUSHI; N TAKAHASHI, Lead, JAPAN SOC BIOSCI BIOTECHN AGROCHEM
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, Apr. 1988, [Reviewed] - BIOLOGICAL CONVERSION OF BENZOIC-ACID IN LEMNA-PAUCICOSTATA-151 AND ITS RELATION TO FLOWER INDUCTION
Y SUZUKI; YAMAGUCHI, I; N MUROFUSHI; N TAKAHASHI, Lead, JAPANESE SOC PLANT PHYSIOLOGISTS
PLANT AND CELL PHYSIOLOGY, Apr. 1988, [Reviewed] - RELATIONSHIP BETWEEN FLOWER INDUCTION BY BENZOIC-ACID AND ITS METABOLISM IN LEMNA-PAUCICOSTATA-151
Y SUZUKI; YAMAGUCHI, I; N MUROFUSHI; N TAKAHASHI, Lead, JAPANESE SOC PLANT PHYSIOLOGISTS
PLANT AND CELL PHYSIOLOGY, Apr. 1988, [Reviewed] - IDENTIFICATION OF CASTASTERONE, TYPHASTEROL AND TEASTERONE FROM THE POLLEN OF ZEA-MAYS
Y SUZUKI; YAMAGUCHI, I; T YOKOTA; N TAKAHASHI, Lead, JAPAN SOC BIOSCI BIOTECHN AGROCHEM
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, Dec. 1986, [Reviewed] - IDENTIFICATION OF CASTASTERONE AND BRASSINONE FROM IMMATURE SEEDS OF PHARBITIS-PURPUREA
Y SUZUKI; YAMAGUCHI, I; N TAKAHASHI, Lead, JAPAN SOC BIOSCI BIOTECHN AGROCHEM
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, [Reviewed]
MISC
- Insect gall induction and phytohormones
鈴木義人、徳田誠
Regulation of Plant Growth & Development, 21 Dec. 2023
Lead - Plant hormone production by insects for gall formation.
鈴木義人
化学と生物, 01 Mar. 2014
Lead - ヤナギのゴール形成ハバチにおける植物ホルモン合成，昆虫と自然
鈴木義人
ニューサイエンス社, 30 Dec. 2013, [Invited]
Lead - Study on hormonal plant growth regulation and its immunological control.
Yoshihito Suzuki
Regulation of Plant Growth & Development, 31 May 2008
Lead - 抗体を用いた植物の成長調節：イムノモジュレーション
鈴木義人
化学と生物, 2008
Lead - A gibberellin mimetic peptide recognized by an anti-gibberellin monoclonal antibody
H. Hemmi; T. Murata; S. Nakamura; K. Shimizu; Y. Suzuki; I. Yamaguchi
American Peptide Symposia, 2006 - 生物の教科書の植物ホルモン
鈴木義人
高校理科教育, 2006
Lead - 高等植物におけるアラビノガラクタン蛋白質の構造と機能について
鈴木義人
化学と生物, 2006
Lead - A gibberellin mimetic peptide recognized by an anti-gibberellin monoclonal antibody
H Hemmi; T Murata; S Nakamura; K Shimizu; Y Suzuki; Yamaguchi, I
BIOPOLYMERS, 2005 - 脂溶性化合物のペプチド性ミミック ファージディスプレイ法によって活性型ジベレリンのミミックを取得
鈴木義人
化学と生物, 2005, [Reviewed]
Lead
Books and other publications
- 生物の教科書の植物ホルモン，高校理科教育12
鈴木義人, Single work
大日本図書, 2006 - 植物における抗体生産の現状と展望，抗体エンジニアリングの最前線
鈴木義人, Single work
シーエムシー出版, 2004 - 植物ホルモンのシグナル伝達系，農芸化学の辞典
鈴木義人; 中嶋正敏; 山口五十麿, Joint work
朝倉書店, 2003 - ジベレリン 化学, 植物ホルモンハンドブック
鈴木義人; 山根久和; 山口五十麿; 室伏旭, Joint work
培風館, 1994 - Transformation in Atropa belladonna
Y. Suzuki; Y Kurioka; T. Ogasawara and H. Kamada, Joint work
Springer-Verlag Berlin Heidelberg New York, 1993
Lectures, oral presentations, etc.
- マダラケシツブゾウムシが合成する植物ホルモンの虫瘤形成における役割
土`田 努; 伊澤 勇人; 井野 隆一朗; 佐野 遥太; 二河 成男; 山口 勝司; 重信 秀治; 佐野 孔亮; 別所-上原 奏子; 鈴木 義人
第69回日本応用動物昆虫学会
20250320, 20250322 - Effects of phytohormones on the development of blossom end enlargement in cucumber
Huang, J.; N. Saginuma; Y. Watabe; I. Komatsuzawa; R. Li; C. Cen; J. Satimunnaithum; Y. Tashiro; Y. Suzuki; S. Tanabata; T. Sato
園芸学会令和7年度春季大会
20250320, 20250321 - エンドウヒゲナガアブラムシを用いた昆虫におけるサイトカイニン水酸化酵素の追究
佐藤 渓、鈴木 義人
第59回植物化学調節学会, 01 Nov. 2024
20241031, 20241102 - Investigating the mysteries of gall formation: A study of gall-forming weevil and its symbiotic bacteria
Ryo Murakami; Ryo Ushima; Ryoma Sugimoto; Yota Sano; Ryuichiro Ino; Yoshihito Suzuki; Kanako Bessho-Uehara; Naruo Nikoh; Tsutomu Tsuchida
27th International Congress of Engomoogy, 28 Aug. 2024
20240825, 20240830 - エンドウヒゲナガアブラムシを用いた昆虫におけるサイトカイニン水酸化酵素の探索
佐藤 渓、鈴木 義人
日本農芸化学会2024年度大会, 25 Mar. 2024, 日本農芸化学会
20240324, 20240327 - 昆虫におけるインドール-3-酢酸生合成酵素に関する研究
市川 輝、國岡 祐里、宮田 海、日裏 雄史、浅見 忠男、土田 努、中村 周吾、藤本 瑞、鈴木 義人
日本農芸化学会2024年度大会, 25 Mar. 2024, 日本農芸化学会
20240324, 20240327 - ゴール形成ハバチ（Pontania sp.）のIAA生合成酵素PonAAS2に対する阻害剤の活性評価
日裏 雄史、吉田 響、浅見 忠男、 鈴木 義人
第58回植物化学調節学会, 19 Nov. 2023
20231117, 20231119 - エンドウヒゲナガアブラムシを用いた昆虫におけるサイトカイニン水酸化酵素の探索
佐藤 渓、鈴木 義人
第58回植物化学調節学会, 17 Nov. 2023
20231117, 20231119 - Studies on PonAAS2, an aromatic aldehyde synthase responsible for IAA production in the gall-inducing sawfly (Pontania sp.)
Hiura; T.; Yoshida; H.; Miyata; U.; Asami; T.; Suzuki; Y.
22th International Conference on Plant Growth Substances, 05 Jul. 2023
20230704, 20230708 - 虫こぶ非形成生物へのオーキシン生産能の付与
吉田響,依田真一,��田努,重信秀治,鈴木義人
日本農芸化学会2023年度大会, 15 Mar. 2023, 日本農芸化学会
20230314, 20230317 - ゴール形成ハバチ（Pontania sp.）のIAA生合成酵素PonAAS2の阻害剤探索
日裏 雄史,浅見 忠男,鈴木 義人
第57回植物化学調節学会, 26 Nov. 2022, 植物化学調節学会
20221125, 20221127 - 身近にある虫コブたち，その形成のメカニズムは？
東京大学柏キャンパス一般公開セミナー「植物と昆虫の切っても切れない関係」, 22 Oct. 2022, [Invited]
20221022 - ゴール非形成性動物へのオーキシン生産能の付与
吉田響,鈴木義人,土田努
日本農芸化学会2022年度大会, 18 Mar. 2022, 日本農芸化学会
20220315, 20220318 - 新規モデル昆虫マダラケシツブゾウムシＳｍｉｃｒｏｎｙｘ ｍａｄａｒａｎｕｓを用いた虫瘤形成機構解析の試み
杉本 凌真,村上 涼生,鵜嶋 涼,別所 奏子,若杉 達也,鈴木 義人,土`田 努
日本動物学会中部支部2021年度大会, 04 Dec. 2021, 日本動物学会中部支部
20211204, 20211205 - ゴール形成ハバチ（Pontania sp.)のIAA生合成酵素PonAAS2の解析
宮田海,阿讃坊和也,戸嶋浩明、鈴木義人
植物化学調節学会第56回大会, 13 Nov. 2021, 植物化学調節学会
20211113, 20211114 - 線虫Caenorhabditis elegansへのオーキシン生産能の付与
吉田響,土田努,鈴木義人
植物化学調節学会第56回大会, 13 Nov. 2021, 植物化学調節学会
20211113, 20211114 - ゴール形成ハバチにおけるインドール酢酸生合成に関わる芳香族アルデヒド合成酵素の解析
宮田海,武井麻美,鈴木義人
日本農芸化学会2021年度大会, 19 Mar. 2021, 日本農芸化学会
20210318, 20210321 - 昆虫におけるサイトカイニン生合成に関する研究
村上輝起; 遠藤理夏; 土田努; 徳田誠; 鈴木義人
第55回植物化学調節学会, 15 Nov. 2020
20201115, 20201117 - アサガオの矮性系統キダチの矮性形質の解析
森藤あかね; 菅野裕也; Melisa Acosta Ramirez; 小野公代; 小野道之; 鈴木義人
第54回植物化学調節学会大会, 15 Nov. 2019
20191115, 20191117 - 昆虫における内生オーキシン量の雌雄別・発育段階別・組織別の比較
松田浩輝; 鈴木義人; 徳田 誠
第54回植物化学調節学会大会, 15 Nov. 2019
20191115, 20191117 - ゴール形成昆虫ハバチによるインドール酢酸生合成酵素に関する研究.
小暮奨太; 武井麻美; 伊藤晋作; 上妻由章; 鈴木義人
第54回植物化学調節学会大会, 15 Nov. 2019
20191115, 20191117 - 陸生節足動物における植物ホルモン（オーキシン・サイトカイニン）内生量の比較および植食性・ゴール形成性との関連
徳田誠; 鈴木義人; 藤田 将平; Ayman Elsayed; 松田 浩輝
第63回日本応用動物昆虫学会大会, 27 Mar. 2019 - 昆虫におけるインドール酢酸生合成酵素の同定
武井麻美; 小暮奨太; 伊藤晋作; 鈴木義人
日本農芸化学会2019年度大会, 27 Mar. 2019 - 節足動物における植物ホルモン内生量の比較および植食性・ゴール形成性の進化との関連
徳田 誠; 鈴木義人; 藤田将平
第66回日本生態学会大会, 17 Mar. 2019 - Transposon insertion in GA3-oxidase gene causing dwarfism of Ipomoea nil strain Kidachi
Melisa Acosta Ramirez; Kimiyo Sage-Ono; Eiji Nitasaka; Nobuyoshi Nakajima; Kenta Shiraiwa; Yoshihito Suzuki; Michiyuki Ono.
第10回アサガオ研究集会, 03 Mar. 2019 - 矮性系統アサガオ「木立」の内生ジベレリンの分析
菅野裕也; Melisa Acosta Ramirez; 小野公代; 小野道之; 鈴木義人
第10回アサガオ研究集会, 03 Mar. 2019 - Acosta Ramirez Melisa, Kimiyo Sage-Ono, Seika Motoyama, Nobuyoshi Nakajima, Kenta Shirasawa, Yoshihito Suzuki and Michiyuki Ono
Acosta Ramirez Melisa; Kimiyo Sage-Ono; Seika Motoyama; Nobuyoshi Nakajima; Kenta Shirasawa; Yoshihito Suzuki and Michiyuki Ono
第41回日本分子生物学会, 28 Nov. 2018 - 節足動物における植物ホルモン(IAA, CKs）内生量の比較および植食性・ゴール形成性との関連
徳田 誠; 鈴木義人; 藤田将平
第53回植物化学調節学会, 02 Nov. 2018 - Effects of Riptortus pedestris infestation on the phenology and physiology of the present and next generations of soybean and Glycine soja
Effects of Riptortus pedestris infestation on the phenology and physiology of the present and next generations of soybean and Glycine soja
第62回日本応用動物昆虫学会大会, 27 Mar. 2018 - 昆虫による植物ホルモン生産とゴール形成
鈴木義人
日本農芸化学会2018年度大会シンポジウム, 18 Mar. 2018, 日本農芸化学会 - 節足動物における植物ホルモン（オーキシン、サイトカイニン）内生量の比較および植食性の進化との関連
節足動物における植物ホルモン(オーキシン; サイトカイニン; 内生量の比較および植食性の進化との関連
日本生態学会第65回全国大会, 17 Mar. 2018 - 有用芳香族アミノ酸代謝物の発酵食品における分布と食品微生物による生産性に関する研究
木村優花; 青木玲二; 高山喜晴; 鈴木チセ; 鈴木義人
日本農芸化学会2018年度大会, 17 Mar. 2018 - 昆虫におけるインドール酢酸の生合成の機構に関する研究
武井麻美; 小暮奨太; 横山千晃; 上妻由章; 鈴木義人
日本農芸化学会2018年度大会, 16 Mar. 2018 - Endogenous levels of phytohormone auxin and cytokinins in arthropods and implications for the evolution of phytophagous and gall inducting habits in insects
Tokuda; M.; Suzuki; Y.; Fujita; S.; Adachi; S.; Hattori; M.; Sobagaki; K.
7th INTERNATIONAL SYMPOSIUM ON CECIDOLOGY, 08 Mar. 2018 - ホソヘリカメムシによる花芽や種子の吸汁がダイズとツルマメに及ぼす影響
中林ゆい; 安達修平; 藤田将平; 鈴木義人; 遠藤信幸; 徳田誠
九州病害虫研究会第94回研究発表会, 08 Nov. 2017 - 昆虫におけるインドール酢酸生合成経路の検証と生合成酵素の同定
小暮奨太; 横山千晃; 武井麻美; 上妻由章; 鈴木義人
第52回植物化学調節学会, 28 Oct. 2017 - 昆虫体内おける植物ホルモン内生量の比較およびニセダイコンアブラムシ体内における急速なtZRの蓄積
徳田 誠; 鈴木義人; 安達修平; 藤田将平
第52回植物化学調節学会, 28 Oct. 2017 - Rapid accumulation of a cytokinin precursor in aphid body and implications for host plant manipulation by aphids
Makoto Tokuda; Yoshihito Suzuki; Muryati; Shohei Fujita; Shuhei Adachi
10th International symposium on aphids, 07 Sep. 2017 - 昆虫体内おける植物ホルモン内生量の比較およびアブラムシ体内における急速なサイトカイニン前駆 物質合成の可能性
徳田 誠; 鈴木義人; 藤田将平; 安達修平
日本昆虫学会第77回大会, 04 Sep. 2017 - 発酵食品における有用芳香族アミノ酸代謝物の分布
木村優花; 青木玲二; 高山喜晴; 鈴木チセ; 鈴木義人
日本農芸化学会関東支部2017年度支部大会, 02 Sep. 2017 - 昆虫による植物ホルモン生産とゴール形成
鈴木義人
第35回日本植物細胞分子生物学会（さいたま）大会, 31 Aug. 2017, [Invited] - 昆虫におけるインドール酢酸の生合成に関する研究
横山千晃; 永田晋治; 上妻由章; 鈴木義人
日本農芸化学会2017年度大会, 20 Mar. 2017 - A study on adaptive significance of gall formation for an aphid inducing galls on Japanese elm trees
Mami Takei; Shinsaku Ito; Taichiro Ishige; Keisuke Tanaka; and Yoshihito Suzuki
22nd International Plant Growth Substances Association, 23 Jun. 2016 - Insect-producing auxin involved in insect gall induction
Yoshihito Suzuki; Hiroyoshi Suzuki; Chiaki Yokohama; Shinji Nagata; and Tadao Asami
22nd International Plant Growth Substances Association, 23 Jun. 2016 - 刈込みにより獲得されたスズメノカタビラの矮性形質に関する研究
鈴木宏佳; 岡崎麻衣子; 小笠原勝; 鈴木義人
日本農芸化学会2016年度大会, Mar. 2016 - トチュウに含まれる癌幹細胞特異的阻害剤Eucommicin Aの同定
藤原綾香; 西真由子; 吉田茂男; 長谷川守文; 安間智慧子; 梁明秀; 鈴木義人
日本農芸化学会2016年度大会, Mar. 2016 - オカボノクロアブラムシが形成するハルニレゴールの適応的意義に関する研究
武井麻美; 伊藤晋作; 石毛太一郎; 田中啓介; 鈴木義人
日本農芸化学会2016年度大会, Mar. 2016 - 昆虫におけるインドール酢酸生合成過程に関する研究
横山千晃; 永田晋治; 鈴木義人
日本農芸化学会2016年度大会, Mar. 2016 - 昆虫におけるインドール酢酸の生合成過程に関する研究
横山千晃; 鈴木義人
第50回植物化学調節学会, 24 Oct. 2015 - オカボノクロアブラムシによるハルニレゴール形成の適応的意義に関する研究
武井麻美; 伊藤晋作; 石毛太一郎; 田中啓介; 鈴木義人
日本農芸化学会2015年度大会, 27 Mar. 2015 - ヒゲブトトガリキジラミの生活史および虫えい形成機構
甲斐進也; 神代 瞬; 安達修平; 鈴木義人; 塩見宜久; 徳田 誠
平成26年度日本昆虫学会九州支部大会, 06 Dec. 2014, 日本昆虫学会九州支部 - オカボノクロアブラムシによるハルニレゴール形成の適応的意義に関する研究
武井麻美; 伊藤晋作; 石毛太一郎; 田中啓介; 仲下英雄; 鈴木義人
第49回植物化学調節学会, 18 Oct. 2014, 植物化学調節学会 - 昆虫におけるインドール酢酸生合成
鈴木宏佳; 横倉淳平; 伊藤つかさ; 永田晋治; 浅見忠男; 鈴木義人
日本農芸化学会2014年度大会, 28 Mar. 2014, 日本農芸化学会 - Characterization of gall tissue formed by an aphid on elm leaves.
武井麻美; 吉田彩夏; 川合隆史; 鈴木義人
第48回植物化学調節学会, 31 Oct. 2013 - シバヤナギにゴールを形成するハバチのサイトカイニン合成能の検討
山口大貴; 貫井光太; 鈴木義人
日本農芸化学会2013年度大会, 25 Mar. 2013 - オカボノクロアブラムシがハルニレに形成する虫えい（ハルニレハフクロフシ）組織の性状解析
吉田彩夏; 川合隆史; 鈴木義人
日本農芸化学会2013年度大会, 25 Mar. 2013 - ハバチによるシバヤナギのゴール（ハウラタマフシ）形成における植物ホルモンの役割
山口大貴; 田中弘毅; 徳田誠; 浅見忠男; 鈴木義人
日本農芸化学会2012年度大会, 24 Mar. 2012 - タマバエによるゴール形成における植物ホルモン類の関与
田中祐一朗; 岡田浩一; 徳田誠; 浅見忠男; 鈴木義人
日本農芸化学会2012年度大会, 24 Mar. 2012 - アブラムシによるハルニレのゴール形成機構に関する研究
吉田彩夏; 鈴木義人
日本農芸化学会2012年度大会, 24 Mar. 2012 - シバヤナギハウラタマフシにおけるゴール形成機構に関する研究
山口大貴; 田中弘毅; 徳田誠; 浅見忠男; 鈴木義人
第46回植物化学調節学会, 01 Nov. 2011 - 昆虫に含まれる内生インドール-3-酢酸の起源
横倉淳平; 山口大貴; 永田晋治; 鈴木義人
第46回植物化学調節学会, 01 Nov. 2011 - ストリゴラクトン新規機能発現因子の探索
長江未有; 増口潔; 上野琴巳; 嶋田幸久; 中野雄司; 中村英光; 米山弘一; 森昌樹; 鈴木義人; 浅見忠男
日本農芸化学会2011年度大会, 26 Mar. 2011 - シバヤナギハウラタマフシにおける虫えい形成機構の研究
山口大貴; 田中弘毅; 浅見忠男; 徳田誠; 鈴木義人
日本農芸化学会2011年度大会, 26 Mar. 2011 - ヨモギハエボシフシにおける虫えい形成機構に関する研究
岡田浩一; 山口大貴; 徳田誠; 浅見忠男; 鈴木義人
日本農芸化学会2011年度大会, 26 Mar. 2011 - Studies on the gall formation mechanism in gall midge-induced Artemisia galls.
Koichi Okada; Hiroki Yamaguchi; Makoto Tokuda; Tadao Asami; Yoshihito Suzuki
第45回植物化学調節学会, 01 Nov. 2010 - Studies on the gall formation mechanism in sawfly-induced willow galls.
Hiroki Yamaguchi; Hiroki Tanaka; Makoto Tokuda; Tadao Asami; Yoshihito Suzuki
第45回植物化学調節学会, 01 Nov. 2010 - Studies on the strigolactone-signaling genes in Arabidopsis.
長江未有; 増口潔; 上野琴巳; 佐々木江里子; 嶋田幸久; 中野雄司; 中村英光; 米山弘一; 森昌樹; 鈴木義人; 浅見忠男
第45回植物化学調節学会, 01 Nov. 2010 - 抗BRI1抗体のスクリーニングとその応用
加藤久美子; 中村郁子; 中野雄司; 浅見忠男; 鈴木義人
日本農芸化学会2010年度大会, 29 Mar. 2010 - ストリゴラクトン生合成阻害剤の開発
伊藤晋作; 北畑信隆; 梅原三喜久; 花田篤志; 上野琴巳; 増口潔; 鈴木義人; 米山弘一; 経塚淳子; 森昌樹; 山口信次郎; 浅見忠男
日本農芸化学会2010年度大会, 29 Mar. 2010 - カロテノイド酸化開裂酵素を標的としたストリゴラクトン生合成阻害剤の探索
加藤敦隆; 北畑信隆; 伊藤晋作; 上野琴巳; 米山香織; 鈴木義人; 米山弘一; 経塚淳子; 中野雄司; 浅見忠男
日本農芸化学会2010年度大会, 29 Mar. 2010 - イムノモジュレーションにより見出されたフィラメント状小胞体の応用
岡田浩一; 浦上恵理子; 浅見忠男; 鈴木義人
第44回植物化学調節学会, 2009 - 抗BRI1抗体のスクリーニングとその応用
加藤久美子; 中村郁子; 中野雄司; 浅見忠男; 鈴木義人
第44回植物化学調節学会, 2009 - 枝分かれに関するジベレリンとストリゴラクトンの効果
伊藤晋作; 梅原三喜久; 花田篤志; 北畑信隆; 鈴木義人; 経塚淳子; 山口信次郎; 浅見忠男
第44回植物化学調節学会, 2009 - カロテノイド酸化開裂酵素を標的としたストリゴラクトン生合成阻害剤の探索
加藤敦隆; 北畑信隆; 伊藤晋作; 上野琴巳; 米山香織; 鈴木義人; 米山弘一; 経塚淳子; 中野雄司; 浅見忠男
第44回植物化学調節学会, 2009 - シロイヌナズナにおけるストリゴラクトン応答性遺伝子の解析
長江未有; 増口潔; 上野琴巳; 佐々木江理子; 嶋田幸久; 中野雄司; 中村英光; 米山弘一; 鈴木義人; 浅見忠男
第44回植物化学調節学会, 2009 - ストリゴラクトン（SL）生合成阻害剤の開発
伊藤晋作; 北畑信隆; 加藤敦隆; 花田篤志; 米山香織; 梅原三喜久; 鈴木義人; 米山弘一; 経塚淳子; 山口信次郎; 浅見忠男
日本農芸化学会2009年度大会, 2009 - 新規骨格を有するストリゴラクトン生合成阻害剤の探索
加藤敦隆; 北畑信隆; 伊藤晋作; 上野琴巳; 花田篤志; 米山香織; 梅原三喜久; 鈴木義人; 米山弘一; 経塚淳子; 山口信次郎; 中野雄司; 浅見忠男
日本農芸化学会2009年度大会, 2009 - ストリゴラクトン応答性遺伝子の探索と機能解析
増口潔; 佐々木江里子; 嶋田幸久; 中野雄司; 米山弘一; 鈴木義人; 浅見忠男
日本農芸化学会2009年度大会, 2009 - ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究
増口潔; 浦上恵理子; 長谷川守文; 三宮一宰; 松本一郎; 山口五十麿; 浅見忠男; 鈴木義人
日本農薬学会34回大会, 2009 - 抗ジベレリン抗体に対する抗メタタイプペプチドのスクリーニング
稲葉絢子; 中村周吾; 清水謙多郎; 浅見忠男; 鈴木義人
日本農薬学会34回大会, 2009 - Regulation of plant growth by immunomodulational approach
Yoshihito Suzuki
2008 Annual Meeting of the Korean Society for Applied Biological Chemistry, 2008, [Invited] - ストリゴラクトン（MAX因子）生合成阻害剤の探索
伊藤晋作; 加藤敦隆; 花田篤志; 米山香織; 北畑信隆; 梅原三喜久; 鈴木義人; 米山弘一; 山口信次郎; 浅見忠男
第43回植物化学調節学会, 2008 - 新規イネジベレリン2-酸化酵素OsGA2ox7の遺伝子発現部位とその基質特異性の解析
中村英光; 朴昇げん; 田切明美; 岡田恵子; 羽方誠; 土岐精一; 中嶋正敏; 鈴木義人; 浅見忠男; 市川裕章
第43回植物化学調節学会, 2008 - 抗活性型ジベレリン抗体の抗メタタイプペプチドの解析
稲葉絢子; 中村周吾; 清水謙多郎; 浅見忠男; 鈴木義人
第43回植物化学調節学会, 2008 - イムノモジュレーションにより見いだされたフィラメント状小胞体の解析
浦上恵理子; 山口五十麿; 浅見忠男; 鈴木義人
日本農芸化学会2008年度大会, 2008 - 抗活性型ジベレリン抗体の抗メタタイプペプチドのスクリーニング
稲葉絢子; 浅見忠男; 鈴木義人
日本農芸化学会2008年度大会, 2008 - GA24/19に対する抗体を用いたイムノモジュレーション
浦上恵理子; 山口五十麿; 浅見忠男; 鈴木義人
第49回日本植物生理学会年会, 2008 - 抗ジベレリン抗体を用いた植物の免疫学的成長調節に関する2つの様式の比較研究
鈴木義人; 浦上恵理子; 山口五十麿; 浅見忠男
日本農薬学会33回大会, 2008 - Defense-related signaling by interaction of arabinogalactan-proteins and b-glucosyl Yariv reagent inhibits gibberellin signaling in barley aleurone cells.
K. Mashiguchi; E. Urakami; M. Hasegawa; K. Sanmiya; I. Matsumoto; I. Yamaguchi; T. Asami and Y. Suzuki
19th International Conference on Plant Growth Substances, Jul. 2007 - Immunomodulational study of gibberellins in plants.
Y. Suzuki; T. Mizuno; I. Yamaguchi and T. Asami
19th International Conference on Plant Growth Substances, Jul. 2007 - 抗活性型ジベレリン抗体を用いた植物の免疫学的機能改変
鈴木義人; 水野徹; 山口五十麿; 浅見忠男
日本農薬学会32回大会, 2007 - Sequential regulation of biosyntheses of gibberellins, brassinosteroids and jasmonic acid operates in rice coleoptiles to control transcript levels of anti-microbial thionin genes.
Y. Suzuki; Y. Kitanaga; K. Sai; J. Yazaki; N. Kisimoto; S. Kikuchi; H. Nakamura; H. Ichikawa; T. Asami; S. Yoshida; I. Yamaguchi
International Chemical Congress of Pacific Basin Societies 2005, 2005 - Isolation and identification of arabinogalactan proteins and novel b-glucosyl Yariv-reactive proteins from rice seeds.
K. Mashiguchi; Y. Suzuki and I. Yamaguchi
International Chemical Congress of Pacific Basin Societies 2005, 2005 - Glycoproteins having arabinogalactan carbohydrate chains for plant growth
Y. Suzuki
Xth France-Japan Workshop on Plant Sciences, 2005, [Invited] - Molecular characterization of highly homologous two receptor-like kinase genes, RLK902 and RKL1, from Arabidopsis thaliana.
Y. Tarutani; T. Morimoto; A. Sasaki; M. Yasuda; H. Nakashita; S. Yoshida; I. Yamaguchi and Y. Suzuki
18th International Conference on Plant Growth Substances, 2005 - Arabinogalactan-proteins in rice seeds possibly involved in gibberellin signaling.
K. Mashiguchi; Y. Suzuki and I. Yamaguchi
18th International Conference on Plant Growth Substances, 2004 - A dwarf mutant strain of Japanese morning glory (Phrbitis nil), uzukobito, has defective brassinosteroid biosynthesis.
Y. Suzuki1; K. Saso; S. Fujioka; S. Yoshida; E. Nitasaka; S. Nagata1; H. Nagasawa; S. Takatsuto; I. Yamaguchi
30th annual meeting of Plant Growth Regulation Society of America, 2003
Research Themes
- マダラケシツブゾウムシ超入れ子型共生系を用いた虫瘤形成機構の包括的解析
基盤研究(B)
Apr. 2021 - Mar. 2025 - Transgenerational changes in pod maturation phenology infested by the bean bug
Grant-in-Aid for Challenging Research (Exploratory)
Saga University
Jul. 2020 - Mar. 2022 - ゴール形成昆虫による植物ホルモン生産能の進化的獲得機構の解明
基盤B
Apr. 2018 - Mar. 2021 - 昆虫における植物ホルモン合成能の獲得が植食性の進化に及ぼしたインパクトの検証
基盤研究(B)
Apr. 2017 - Mar. 2020 - 昆虫におけるオーキシン生合成酵素の分子進化とゴール形成能獲得の因果性の解明
基盤研究(C)
Apr. 2015 - Mar. 2018 - Development of the Medical, Food and Agricultural Integrated Sector Based on Rural Society
Grant-in-Aid for Scientific Research (B)
Ibaraki University
Apr. 2013 - Mar. 2016 - イムノモジュレーションを用いた植物細胞膜タンパク質の網羅的機能解析
Grant-in-Aid for Scientific Research(B)
Ibaraki University
Apr. 2010 - Mar. 2013 - 植物ホルモンを認識する抗体を用いた不稔性植物の開発
JSTシーズ発掘試験研究
Apr. 2008 - Mar. 2009 - 穀類種子におけるジベレリン情報伝達に関与する糖タンパク質の生理学的解析
Grant-in-Aid for Scientific Research(B)
The University of Tokyo
Apr. 2005 - Mar. 2007 - TRALNSFORMATION OF PLANTS WITH ANTI-PLANT HROMONE ANTIBODY
Grant-in-Aid for Scientific Research (B).
THE UNIVERSITY OF TOKYO
1997 - 1999 - Synthesis of DNA coding the amino acid sequence in an anti-GA4-antibody responsible for the recognition of GA and its application.
Grant-in-Aid for General Scientific Research (B)
The University of Tokyo
1993 - 1995
Academic Contribution Activities
- Int J Mol Sci査読
Peer review
Mar. 2025 - 日本農学進歩賞授賞選考
Review
30 Sep. 2024 - Insectsの査読
Peer review
Sep. 2024 - Insectsの査読
Peer review
Jul. 2024 - Arthropod-Plant Int査読
Peer review
Jul. 2024 - 日本農学賞・読売農学賞 選考
Review
10 Feb. 2024 - 日本農学進歩賞授賞選考
Review
27 Sep. 2023 - 公開 Bioscience, Biotechnology, and Biochemistryの査読
Peer review
Sep. 2023 - Arthropod-Plant Int査読
Peer review
Aug. 2023 - 日本農学賞・読売農学賞 選考
Review
11 Feb. 2023 - Biomoleculesの査読
Peer review
Jan. 2023 - Appl Entomol Zoolの査読
Peer review
Nov. 2022 - 日本農学進歩賞授賞選考
Review
03 Oct. 2022 - Plantsの査読
Peer review
09 Aug. 2022 - Insectsの査読
Peer review
May 2022 - BMC Plant Biol.の査読
Peer review
May 2022 - 日本農学賞・読売農学賞 選考
Review
11 Feb. 2022 - J. Ins. Physiol.の査読
Peer review
Sep. 2020 - SN Applied Sciencesの査読
Peer review
Aug. 2020 - Bioscience, Biotechnology, and Biochemistryの査読
Peer review
Jul. 2020 - Frontiers Plant Scienceの査読
Peer review
Mar. 2020 - Plantsの査読
Peer review
Dec. 2019 - Bioscience, Biotechnology, and Biochemistryの査読
Peer review
Oct. 2019 - Frontiers Plant Scienceの査読
Peer review
Aug. 2019 - 日本学術振興会 特別研究員等審査会専門委員，卓越研究員候補者選考委員会書面審査員および国際事業委員会書面審査員・書面評価員
Review
01 Aug. 2018 - 30 Jun. 2019 - Frontiers Plant Scienceの査読
Peer review
Jun. 2019 - J.Chem. Ecol.の査読
Peer review
Mar. 2019 - 日本農芸化学会 農芸化学女性研究者賞等授賞選考委員
Review
07 Jul. 2016 - 28 Feb. 2019 - J. Pesticide Sci.の査読
Peer review
Dec. 2016 - J. Pesticide Sci.の査読
Peer review
Feb. 2016 - J. Pesticide Sci.の査読
Peer review
Jan. 2016 - PlosOneの査読
Peer review
Oct. 2015 - J. Pesticide Sci.の査読
Peer review
Jun. 2015 - Floraの査読
Peer review
Mar. 2014 - 日本農芸化学会関東支部例会「生物個体間の化学 最近の話題から」
Planning etc
09 Feb. 2013 - Anal. Chem.の査読
Peer review
Nov. 2012 - J. Plant Growth Regul.の査読
Peer review
Jan. 2012 - J. Plant Growth Regul.の査読
Peer review
Dec. 2011 - 日本学術振興会科学研究費委員会専門委員
Review
01 Dec. 2009 - 30 Nov. 2011 - Bioscience, Biotechnology, and Biochemistryの査読
Peer review
Aug. 2011 - Bioscience, Biotechnology, and Biochemistryの査読
Peer review
Sep. 2010 - Bioscience, Biotechnology, and Biochemistryの査読
Peer review
May 2010 - シンポジウム企画「不毛な土地での植物生産」
Planning etc
Mar. 2010 - Anal. Biochem.の査読
Peer review
Nov. 2009 - Febs Lett.の査読
Peer review
Sep. 2007 - BMC Plant Biol.の査読
Peer review
Aug. 2005 - BMC Plant Biol.の査読
Peer review
Dec. 2004
