Takuya KANAZAWAAssociate Professor

■Researcher basic information

Organization

  • College of Agriculture Department of Food and Life Science
  • Graduate School of Agriculture( Master's Program) Major in Agricultural Science Course in Practical Agricultural Food Science
  • Faculty of Applied Biological Science Domain of Food and Life Science

Research Areas

  • Life sciences, Developmental biology, Developmental biology
  • Life sciences, Cell biology, Animal Cell Science and Genetics
  • Life sciences, Zoological sciences, Integrative animal science
  • Life sciences, Cell biology, Cell biology
  • Life sciences, Laboratory animal science, Integrative animal science
  • Life sciences, Animals: biochemistry, physiology, behavioral science, Integrative animal science

Research Keyword

  • Animal Breeding, Cell Biology, Developmental Biology, Mammary gland, Mammary fat pad, Testis, Meiosis, Gene polymorphism, Metabolic syndrome, Skeletal muscle

Degree

  • 1989年06月 農学博士(東京大学)

Educational Background

  • 1989, The University of Tokyo, Graduate School, Division of Agricultural Science, 畜産獣医学

Career

  • Apr. 2016, 茨城大学, 農学部, 准教授

Member History

  • 05 Nov. 2016, President, The Society for Biology of Mammary Gland and Lactation
  • Nov. 2013 - Nov. 2016, 代表, 乳腺・泌乳研究会
  • Nov. 2016, President, The Society for Biology of Mammary Gland and Lactation

Message from Researchers

  • (Message from Researchers)

    (研究経歴)
    1. Regulation of growth and differentiation of mouse mammary epithelial cells---Development and application of systems of serum-free cell culture and Western blotting for casein analysis. (1984-1989) ,2. Role of stromal cells in the induction of neoplastic transformation in the primary mouse mammary epithelial cells. (1989-1991),3. Molecular mechanisms for activation of phosphatidylinositol 3-kinase by Ineterleukin 2-receptor. (1992-1993),4. Immunohistological study on pathological changes in folicular dendritic cells of apes infected with simian immunodeficiency virus. (1993-1994),5. Molecular and cell biological analysis for marbled beef development. (1994- ),6. Molecular and cellular analysis for growth and differentiation of testicular germ cells of the ruminant and the mouse. (1995- ),7. Survey for suppressory substances for mouse mammary tumor. (2001- ),8. Analysis of metabolic syndrome using a newly established mouse model system. (2006-),9. Molecular and cellular analysis of specificity of mammary fad pad. (2009-)

■Research activity information

Paper

  • Simultaneous isolation of two species, Brachyspira pilosicoli and Brachyspira aalborgi, from a patient with ulcerative colitis
    Kanazawa T et al, We succeeded in the simultaneous isolation of Brachyspira (B.) aalborgi and B. pilosicoli from a patient with ulcerative colitis. B. pilosicoli grew quickly and formed colonies within 7 days, while the growth of B. aalborgi was very slow and took over 21 days. Simultaneous isolation of B. pilosicoli and B. aalborgi from a common specimen is generally recognized to be difficult, mainly due to differences in their growth requirements and the growth rates. However, we succeeded in isolating both species from a patient with ulcerative colitis and this is first evidence. The present results suggest that ulcerative colitis may be caused by simultaneous infection with B. pilosicoli and B. aalborgi., The Japanese Society of Veterinary Science
    Journal of Veterinary Medical Science, 07 Aug. 2018, [Reviewed]
  • Genotype analysis of partial growth hormone gene (GH891/Msp1) in Pesisir cattle and Simmental-Pesisir crossbred cattle
    T. Hartatik; D. E. Putra; S. D. Volkandari; T. Kanazawa and Sumadi, Growth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI-And MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH's genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891MspI) in Pesisir and SimPes (Simmental x Pesisir) cattle population were found and no significant effect on growth trait performance., Indonesian Society of Animal Agriculture
    Journal of the Indonesian Tropical Animal Agriculture, Mar. 2018, [Reviewed]
  • Short communication: Identification of growth hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia
    Dino Eka Putra; Sumadi; Takuya Kanazawa; Tety Hartatik, This study aimed to determine gene polymorphism of growth hormone of domestic cattle in Pesisir Selatan District of West Sumatra. The research was conducted at Laboratory of Animal Genetics and Breeding Faculty of Animal Science, UGM from August 2013 to January 2014. Blood samples were collected from 66 individuals consist of 15 Pesisir cattle, 15 SimPes cattle, 15 SimPO cattle, 15 Bali cattle and 6 PO cattle. DNA was extracted from each blood samples after SDS-proteinase K digestion, and used for PCR-amplification for a region of growth hormone gene (211 bp), and then the PCR products were analyzed for restriction fragment length polymorphism (RFLP) using AluI restriction enzyme. The results showed that GH gene of Pesisir, PO and Bali cattle are monomorphic, which frequencies of L allele was 1.00 and V allele was 0.000 while these LL genotype was 1.000. Frequency of L and V alleles in SimPO and SimPes cattle were 0.634, 0.366 and 0.700, 0.300, respectively. SimPO and SimPes cattle were polymorphic, LL and LV of SimPO cattle was 0.733 and 0.267 as well as SimPes cattle which LL and LV was 0.600 and 0.400, respectively. The correlation between genotype and the performance (body weight and body size) was not significant. The present study indicates that polymorphism of growth hormone gene in AluI site could not yet be used as a molecular marker for body weight and body size of beef cattle., Society for Indonesian Biodiversity
    Biodiversitas, 01 Oct. 2016, [Reviewed]
  • Drug-susceptibility of isolates of Brachyspira hyodysenteriae isolated from colonic mucosal specimens of pigs collected from slaughter houses in Japan in 2009
    Keita Kajiwara; Midori Kozawa; Takuya Kanazawa; Kouji Uetsuka; Hiromi Nakajima; Yoshikazu Adachi, Twenty nine isolates identified as Brachyspira hyodysenteriae were most susceptible to carbadox and metronidazole, whereas they were resistant to macrolides. The isolates showed intermediate susceptibility to tiamulin, lincomycin, penicillin G, ampicillin, chloramphenicol, tetracycline, enrofloxacin and valnemulin, with MIC50 values ranging from 0.39 to 3.13., JAPAN SOC VET SCI
    JOURNAL OF VETERINARY MEDICAL SCIENCE, Mar. 2016, [Reviewed]
  • Suppressive effects of ovarian steroid hormones on the expression of synaptonemal complex protein 1 in organ cultured testis of neonatal mice               
    Takuya KANAZAWA, Ovarian steroid hormones, id est, progesterone and beta-estradipol, were shown to suppress the expression of synaptonemal complex protein 1 gene and protein in the organ-cultured testis of neonatal mice, reversibly, which results would account for roles of these hormones in suppression of meiosis of spermatogonial cells until birth.
    世界繁殖生物学会議2014, 03 Sep. 2014, [Reviewed]
  • ミトコンドリアDNAのD-loop領域の塩基配列上のHinf Iサイトを利用したPCR-RFLP法によるリュウキュウイノシシ(Sus scrofa riukiuanus)としての簡易鑑別               
    林(長濱)ちひろ
    日本畜産学会報, May 2013, [Reviewed]
  • In vitro susceptibility of 73 isolates of Aeromonas hydrophilia to 20 antimicrobial agents in American catfish               
    Kazi Md. Azimuddin; Takuya Kanazawa; Yoshikazu Adachi
    Japanese Journal of Animal Hygiene, Feb. 2010, [Reviewed]
  • Effects of NaCl ,salinity on the sugar metabolism of common bean (Phaseolus ,vulgaris L.) cv. 'Tsurunashi Marusaya Kurosando' fruit grown,in solution culture.               
    Tazuke; A.; T. Wada and T. Kanazawa
    J. ISSAAS, May 2009, [Reviewed]
  • Comparison of the hemagglutination of formalinized American Channel Catfish (Ictalurus punctatus) erythrocytes between formalinized and live Aeromonas hydrophila isolated from the catfish rearing in Kasumigaura Lake in Japan
    Kazi Md. Azimuddin; Kazuhiro Hayakawa; Takuya Kanazawa; Yoshikazu Adachi, Formalinized Aeromonas (A.) hydrophila agglutinated loosely with the formalinized American channel catfish erythrocytes (FACCE), while live A. hydrophila agglutinated tightly with the FACCE. There was a significant difference on the number of attaching bacterial cells to the FACCE (p<0.01) (n=40 erythrocytes) between formalinized and live A. hydrophila. The other bacteria such as Salmonella (S.) Typhimurium ST-5, Escherichia (E.) coli V-517 and Staphylococcus (S.) hyicus ATCC1249 used in this experiment did not attach the FACCE., JAPAN SOC VET SCI
    The Journal of Veterinary Medical Science, Jan. 2008, [Reviewed]
  • Comparison of the hemagglutination features between formalinized American Channel Catfish (Ictalurus punctatus) erythrocytes and formalinized chicken erythrocytes with Aeromonas hydrophilia isolated from the catfish rearing Kasumigaura Lake in Japan               
    Kazi Md. Azimuddin; Kazuhiro Hayakawa; Takuya Kanazawa; Yoshikazu Adachi
    The Japanese Journal of Animal Hygiene, Sep. 2007, [Reviewed]
  • Casein expression in heterotopic organs of lactating mice               
    Takuya Kanazawa; Akinobu Takagai; Howard L. Hosick
    Molecular and Cellular Biology, Dec. 2006
  • Establishment and characterization of cell lines cloned from mammary epithelium of p53-deficient mice               
    Takuya Kanazawa; et al.
    Proceedings of American Association for Cancer Research, Apr. 2002, [Reviewed]
  • Establishment and characterization of cell lines cloned from seminal vesicles of p53-deficient mice.
    T Kanazawa; S Aizawa; Y Tomooka, SOC STUDY REPRODUCTION
    BIOLOGY OF REPRODUCTION, 2002
  • Establishment and characterization of cell lines cloned from seminal vesicles of p53-deficient Mmice
    Takuya Kanazawa; et al., SOC STUDY REPRODUCTION
    Biology of Reproduction, 2002
  • Establishment and characterization of cell lines from mammary epithelium of p53-deficient mice               
    Takuya Kanazawa; et al.
    Proceedings of the American Association for Cancer Research, 2002, [Reviewed]
  • Effects of Co-Cultured Mature Adipocytes on Proliferation and Adipogenic Differentiation of Immature Cells Isolated from Rat Adipose Tissues               
    Yasushi Kasahara; Takuya Kanazawa; Motoaki Kosugiyama
    Animal Science Journal (Japanese with English Summary and Figures), 2002, [Reviewed]
  • Immunochemical demonstration of αs1- and β-casein in mouse mammary glands at early stages of pregnancy
    Takuya Kanazawa; Kaoru Kohmoto, We generated monoclonal antibodies (MAbs) against mouse alpha(s1)- and beta-casein and used them to survey casein immunochemically in mammary glands of mice at pericoitous and pregnant stages. Two MAb-producing hybridoma cells, designated MCalpha1 cell and MCbeta1 cell, were established. Each antibody, when used in Western blotting, recognized specifically mouse alpha(s1)- and beta-casein among a wide spectrum of proteins of both a lactating mammary homogenate and mouse skim milk. Immunohistochemistry revealed alpha(s1)- and beta-casein in sections of lactating mammary glands. Staining was found in substances in the lumen and cytoplasm of duct and alveolar cells, particularly in rough endoplasmic reticulum and the Golgi apparatus. Mammary glands at Days 2, 4, 6, 8, and 14 of pregnancy showed positive staining specific to both alpha(s1)- and beta-casein in the lumen and cytoplasm of duct cells, whereas the glands at estrus and Day 0 of pregnancy were positive mainly for alpha(s1)-casein. Semiquantitative Western blotting analysis of both casein components in epithelial cell fractions from glands during pregnancy confirmed that intra-epithelial alpha(s1)- and beta-casein changed during three phases, elevated from trace levels to detectable levels during initial stages of pregnancy (Days 0, 2, and 4), declined to lower levels during mid-pregnancy (Days 6 and 8), and then rose to high levels during late pregnancy (Day 14)., HISTOCHEMICAL SOC INC
    Journal of Histochemistry and Cytochemistry, 2002, [Reviewed]
  • Effects of 1α,25-dihydroxycholecalciferol and cortisol on the growth and differentiation of primary cultures of mouse mammary epithelial cells in collagen gel
    Takuya Kanazawa; Jumpei Enami; Kaoru Kohmoto, We examined the effects of 1 alpha,25-dihydroxycholecalciferol (1,25-DHCC) and the glucocorticoid, cortisol, on primary mouse mammary epithelial cells in collagen gel cell culture systems. Physiological low concentrations (10(-11)-10(-9) M) of 1,25-DHCC stimulated growth of the cells in a collagen gel matrix culture in serum-free DMEM+Ham's F12 (1:1) medium containing BSA, EGF and cholera toxin, and the cell number reached 1.8-fold the control after 6 d in culture. In contrast, supraphysiological concentrations (10(-8)-10(-7) M) of 1,25-DHCC suppressed cell growth. Cortisol produced similar, but smaller, dose-dependent effects. The addition of serum to the culture medium masked the stimulatory effect of 1,25-DHCC and both the stimulatory and inhibitory effects of cortisol. 1,25-DHCC also affected casein synthesis by cells cultured in a serum-free floating collagen gel culture containing prolactin, insulin and cortisol, enhancing synthesis at low concentrations (10(-11)-10(-9) M) and inhibiting it above 10(-8) M. In the absence of cortisol, no detectable change in casein synthesis was induced by 1,25-DHCC. These results suggest a physiological role for 1,25-DHCC in stimulating both growth and differentiation of mouse mammary epithelial cells, though 1,25-DHCC does not substitute for glucocorticoids in the differentiation of the cells. (C) 1999 Academic Press., ACADEMIC PRESS LTD
    Cell Biology International, 1999, [Reviewed]
  • Phylogenetic relationship of ┣DBCaprini(/)-┫DB estimated by Cytochrome b gene sequence analysis
    Katsumi Arai; Isao Munechika; Isao Ito; Akio Kikkawa; Kojiro Nakamura; Takuya Kanazawa; Motoaki Kosugiyama, 通信著者 Corresponding Author, Japanese Society of Animal Science
    Animal Science and Technology (Current: Animal Science Journal), 1997, [Reviewed]
  • Interactions of hormones and growth factors in the growth and differentiation of p53-deficient mouse mammary cell lines.               
    Shougo Yasaka; Takuya Kanazawa; Motoaki Kosugiyama
    The Proceedings of 8th Asian-Australasian Animal Production Society, 1996
  • Stimulatory effect of vitamin C on the growth of mouse myoblastic cells               
    Takuya Kanazawa; Motoaki Kosugiyama; Ryoichi Matsuda
    Zoological Science, 1996
  • Establishment and characterization of cell lines cloned from seminal vesicles of p53-deficient mice               
    Takuya Kanazawa; et al.
    Zoological Science, 1995
  • Serine-rich region of the IL-2 receptor β-chain is required for activation of phosphatidylinositol 3-kinase
    Takuya Kanazawa; Marilyn Keeler; Lyuba Varticovski, The intracellular portion of the IL-2 receptor (IL-2R) signal transducing beta-chain contains a distinct region, designated ''serine-rich,'' which encompasses sequences required for IL-2-mediated cell growth. Although the receptor does not possess intrinsic protein-tyrosine kinase activity, IL-2 binding induces activation of intracellular protein-tyrosine kinases. Activation of many protein-tyrosine kinases leads to activation of phosphatidylinositol 3-kinase (PI 3-kinase). IL-2 binding also induces activation of PI 3-kinase. To study the interaction of PI 3-kinase with the IL-2 receptor beta-chain we analyzed PI 3-kinase activity in cells which express the wild type and mutant beta-chain. IL-2 mediated an increase in association with PI 3-kinase activity and protein in immunoprecipitates from cells expressing mitogenically competent receptors. PI 3-kinase products also increased in response to IL-2 in these cells. Deletion of the beta-chain serine-rich region abolished IL-2-mediated mitogenesis and cells expressing this mutant failed to activate PI 3-kinase. The interaction of the IL-2 receptor with an intracellular tyrosine kinase, lck, has been mapped to the acidic-rich region of the beta-chain. Cells which express the beta-chain lacking the acidic-rich region grow in the presence of IL-2 and had IL-2-dependent activation of PI 3-kinase. Activation of PI 3-kinase in response to IL-2 was not abolished by treatment of cells with rapamicin and occurred only in cells which express mitogenically competent receptors. The results presented in this study suggest that IL-2-mediated PI 3-kinase activation occurs by a mechanism distinct from interaction with the lck protein-tyrosine kinase. (C) 1994 Academic Press, Inc., ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
    Cellular Immunology, 1994, [Reviewed]
  • IL-2 receptor β chain serine-rich region is required for association with PI 3-kinase
    Takuya Kanazawa; Marilyn Keeler; Lyuba Varticovski, CARDEN JENNINGS PUBL CO LTD
    Experimental Hematology, 1993
  • A co-culture system for studies of paracrine effects of stromal cells on the growth of epithelial cells               
    Takuya Kanazawa; Howard L. Hosisck
    Journal of Tissue Culture Methods, 1992, [Reviewed]
  • Transformed growth phenotype of mouse mammary epithelium in primary culture induced by specific fetal mesenchymes
    Takuya Kanazawa; Howard L. Hosick, When mesenchyme from fetal mammary or salivary gland is implanted into adult mouse mammary gland, adjacent epithelium responds with intense hyperplasia. The hyperplastic cells are more vulnerable than are non-stimulated cells to transformation in vivo by a chemical carcinogen or by mammary tumor virus. This system provides a potentially useful model for determining how stroma contributes to mammary tumorigenesis. We have developed co-culture systems and used them to investigate in more detail the nature of the signal produced by the mesenchyme cells. Monolayers of mesenchyme cells were prepared on tissue-culture wells. The mesenchyme cells were trapped on the surface by a thin overlay of agarose. Primary mammary epithelial cells were cultured atop this barrier layer, either as organoids in collagen gels for assessment of anchorage-dependent growth, or as single-cell dispersions in soft agarose for assessment of anchorage-independent growth. Our procedures for assay of anchorage-independent growth allow us for the first time to detect and measure this transformation-defining characteristic in non-immortalized mammary epithelial cells in primary culture. Fetal mammary fat pad precursor tissue and fetal salivary mesenchyme both stimulated anchorage-dependent growth of mammary epithelium, with cell number increasing as much as fifteenfold during a 6-day culture period. These same fetal tissues also stimulated anchorage-independent growth of the mammary epithelial cells, with colony-forming efficiencies of up to 40% in co-cultures with salivary mesenchyme. No colonies formed in the absence of mesenchyme. Cells of colonies contained keratin, which indicates that the colonies grew from epithelial cells and not from a contaminant of another cell type. When co-cultured epithelial cells were subsequently re-cultured in the absence of mesenchyme, they lost their ability to grow independent of anchorage. No colonies grew in co-cultures with fetal cells from heart, kidney, or lung, which is consistent with the lack of stimulation by these tissues in the mammary gland in vivo. A tumor promoter, 12-0-tetradecanoylphorbol acetate (TPA), also caused anchorage-independent growth of the dispersed mammary epithelial cells. Culture medium conditioned by primary or early-passage salivary mesenchyme cells was capable of stimulating growth under both anchorage-dependent and anchorage-independent conditions, confirming that these effects are mediated by a paracrine factor. The results indicate that stimulatory fetal mesenchymes produce soluble molecules that act analogously to transforming growth factors., WILEY-LISS
    Journal of Cellular Physiology, 1992, [Reviewed]
  • Induction of transformation-like phenotype in primary mouse mammary epithelial cells by fetal and adult virgin mouse mammary stromal cells in culture               
    Takuya Kanazawa; Howard L. Hosick
    Journal of Cell Biology, 1991, [Reviewed]
  • Stimulation of anchorage-dependent and anchorage-independent growth of primary mammary epithelial cells by mouse fetal salivary mesenchyme cells               
    Takuya Kanazawa; Howard L. Hosisck
    Proceedings of the American Association for Cancer Research, 1991
  • Stimulation of mammary epithelium in collagen gel cultures by cell lines of fetal mesenchyme               
    Takuya kanazawa; Howard L. Hosick
    Abstracts for The Sixth International Conference on Differentiation of Normal and Neoplastic Cells, 1990
  • Differentiation of mouse mammary epithelial cells during early stages of pregnancy               
    Takkuya Kanazawa; Kimiko Tatikawa; Kaoru Kohmoto; Howard L. Hosick
    Abstracts for the 6th International Conference on Differentiation of Normal and Neoplastic Cells, 1990
  • Effects of prolactin and progesterone on the growth of mammary epithelial cells in culture               
    Takuya Kanazawa; Kaoru Kohmoto
    Abstracts for the Vth International Congress on Prolactin, 1988
  • Role de la 1a,25-dihydroxyvitamine D3 dans la proliferation et la differenciation fonctionnelle des cellules epitheliaux de la glande mammaire chez la souris(仏語)               
    Takuya Kanazawa; Kaoru Kohmoto
    BULLETIN DE LA SOCIETE FRANCO-JAPONAISE DE BIOLOGIE, 1987

MISC

  • マウス乳腺上皮細胞の増殖および分化に及ぼす間質細胞の役割               
    金澤卓弥
    日仏生物学会誌, 30 Oct. 2012, [Invited]
  • 気候変動の下での土地利用・農牧業システムの研究1               
    中川光弘; 田附明夫; 長澤淳; 金澤卓弥
    ICAS年報2009, Aug. 2009
  • Immunological detection of casein in heterotopic organs of lactating mice               
    Takuya Kanazawa; Akinobu Takagai
    Congress Proceedings CD of the XIIth AAAP Animal Science Congress 2006, 18 Sep. 2006
  • Mechanism of vitamin C stimulation in the growth and differentiation of myoblastic cells               
    Takuya Kanazawa; et al.
    平成9年度食肉に関する助成研究調査報告書, Dec. 1998
  • Effects of vitamin C and vitamin E on the growth and differentiation of Myoblasts               
    Takuya Kanazawa; et al.
    平成8年度食肉に関する助成研究調査報告書, Dec. 1997
  • Establishment of cell lines of presumptive myoblasts and preadipocytes from bovine skeletal muscles               
    Takuya Kanazawa; et al.
    平成7年度食肉に関する助成研究調査報告書, Dec. 1996
  • Control mechanisms for growth and differentiation of mammary epithelial cells---Establishment and application of cell culture systems               
    Takuya Kanazawa
    TPC NEWS, 1994
  • 増殖因子およびステロイドによるマウス乳腺細胞の増殖とカゼイン合成の調節               
    金澤卓弥; 榎並淳平; 河本 馨
    第25回乳腺、泌乳研究会講演集, 1985

Books and other publications

  • 動物学の百科事典               
    金澤卓弥, Contributor
    丸善出版, 30 Sep. 2018
    9784621303092
  • Bulletin de la Societe Franco-Japonaise de Biologie, Tome 52 (French)               
    Takuya KANAZAWA, Single work
    日仏生物学会発行、早崎峯夫編集, 31 Oct. 2012
  • Fetal stromal cells produce α-TGF that elicits some preneoplastic traits in adult mammary epithelium               
    Howard L. Hosisck; ...; Takuya Kanazawa, Joint work
    Hormonal Carcinogenesis, Springer-Verlag, New York, Berlin, Heidelberg, London, Paris, Tokyo, Hong Kong, Barcelona Budapest, 1992
  • Growth and Differentiation of Mouse Mammary Epithelial Cells-Comparison of Normal Mammary Epithelial Cells and an Established Line of Mammary Tumor Cells               
    Takuya Kanazawa; Kaoru Kohmoto, Joint work
    The Tissue Culture, New Science Co, Tokyo, 1986

Lectures, oral presentations, etc.

  • 〔Major achievements〕初代マウス乳腺間質未分化細胞の脂肪細胞分化に及ぼすプロゲステロンおよびエストロゲンの効果               
    金澤卓弥
    第51回乳腺・泌乳研究会研究集会, 23 Mar. 2014, 金澤卓弥(研究会代表)
    20240323, 20240323
  • 器官培養法による新生児マウス精巣内精原細胞の減数分裂能の検証               
    金澤卓弥; 他 学生
    日本動物学会関東支部第71回大会, 09 Mar. 2019, 日本動物学会関東支部
  • 【経過報告】イソブチルメチルキサンチンおよびインドメタシン刺激による初代マウス乳腺間質細胞の脂肪細胞分化過程における脂肪合成関連遺伝発現の定量解析               
    金澤卓弥
    第49回乳腺・泌乳研究会研究集会, 01 Dec. 2018, 乳腺・泌乳研究会
  • マウス各種器官におけるカゼイン発現の解析
    第一著者
    48Th Meeting, The Society of Biology for Mammary Gland and Lactation, 02 Dec. 2017, 乳腺・泌乳研究会
  • Lipid metabolic genes in adipogenic differentiating mouse mammary stromal cells stimulated by indomethacin
    Takuya Kanazawa
    日本畜産学会第123回大会, 06 Sep. 2017, 日本畜産学会
  • Adipocyte differentiation induced by indomethacin and 3-isomethyl-1-butylxanthine in mammary stromal cells of retired mice in primary culture.               
    Takuya Kanazawa
    The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 23 Aug. 2016, AAAP
  • 初代マウス乳腺間質細胞の脂肪細胞分化に及ぼすインドメタシン、イソブチルメチルキサンチンおよびトリヨードサイロニンの影響               
    Kenji FUJIMATA and Takuya KANAZAWA
    第46回乳腺・泌乳研究会研究集会, 05 Dec. 2015, The Society for Biology of Mammary Gland and Lactation
  • Suppressive effects of ovarian steroid hormones on the expression of synaptonemal complex protein 1 in organ-cultured testis of neonatal mice
    Takuya Kanazawa
    World Congress of Reproductive Biology 2014, 03 Sep. 2014, Society for Reproduction and Fertility (host society), Society for Reproductive Biology, Society for Study of Reproduction, Society for Reproduction and Development, Chinese Society for Reproductive Biology, Korean Society for Animal Reproduction
  • 細胞培養系および生体移植系におけるマウス乳腺腫瘍由来株化樹立細胞の増殖に及ぼすニンニクエキスの効果               
    金澤卓弥; 菅良子・Howard; L. Hosick
    第23回乳癌基礎研究会, 27 Aug. 2014, 乳癌基礎研究会
  • 減数分裂開始期のマウス精巣におけるシナプトネマ複合体構成タンパク質遺伝子の定量的発現解析               
    第; 著者
    第106回日本繁殖生物学会大会, 12 Sep. 2013, 日本繁殖生物学会
  • 初代マウス乳腺上皮細胞と各種脂肪由来未分化細胞の間の細胞接着および傍分泌増殖効果の差異               
    単独著者
    第22回乳癌基礎研究会, 21 Jul. 2013, 乳癌基礎研究会
  • 若齢マウス精巣におけるシナプトネマ複合体関連タンパク質遺伝子の発現動態               
    第; 著者
    日本動物学会第65回関東支部大会, 16 Mar. 2013, 日本動物学会関東支部
  • 移植マウス乳腺上皮の形態形成および分化への周囲脂肪の影響               
    単独著者
    第21回乳癌基礎研究会, 22 Jul. 2012, 乳癌基礎研究会
  • 各種脂肪組織由来細胞によるマウス乳腺上皮細胞支持機能の培養内解析               
    単独著者
    日本畜産学会第115回大会, 29 Mar. 2012
  • 器官培養マウス精巣における減数分裂の開始および維持に及ぼすホルモンおよび間質細胞の影響
    第; 著者
    日本動物学会第64回関東支部大会, 17 Mar. 2012, 日本動物学会関東支部会
  • 若齢マウス精巣において特異的に発現する遺伝子の探索               
    菊池亜希; 金澤卓弥
    日本動物学会関東支部第63回大会, 12 Mar. 2011
  • マウス各種脂肪組織による乳腺上皮の形態形成および分化支持能力の検証               
    単独著者
    日本畜産学会第112回大会, 28 Mar. 2010
  • 乳腺脂肪細胞の分子細胞生物学的性状解析ならびに脂肪組織未分化細胞の分化誘導系の開発               
    単独著者
    京都大学再生医科学研究所「再生医学・再生医療の先端融合的共同研究拠点」平成21年度 共同研究会, 26 Mar. 2010, 京都大学再生医科学研究所, [Invited]
  • 乳腺脂肪細胞の分子細胞生物学的性状解析ならびに脂肪組織未分化細胞の分化誘導系の開発               
    単独著者
    京都大学再生医科学研究所「再生医学・再生医療の先端融合的共同研究拠点」平成21年度 共同研究会, 26 Mar. 2010, 京都大学再生医科学研究所, [Invited]
  • Effect of vitamins and minerals on inducibility of metabolic syndrome in mice, and differnce of the inducibility between two mouse strains               
    第; 著者
    Japanse Society for Zoology, Kanto Division, 60th Annual Meeting, 22 Mar. 2008
  • マウス乳腺脂肪組織における胎児乳腺原基および表皮移植片の形態形成、ならびに間充織の影響               
    第; 著者
    日本動物学会関東支部第60回大会, 22 Mar. 2008, 日本動物学会関東支部会
  • 代謝症候群誘導におけるBALB/cJおよびC57BL/6Jマウス系統間の差異               
    第; 著者
    日本動物学会関東支部第59回大会, 24 Mar. 2007, 日本動物学会関東支部
  • マウス膵臓におけるカゼイン発現と一型糖尿病発症との関連性               
    第; 著者
    日本動物学会関東支部第59回大会, 24 Mar. 2007, 日本動物学会関東支部
  • Management factors which affect pruductivity of dairy cattle in Japan               
    単独著者
    Third Japanese-Chinese Joint Symposium "Economic Development and Environmental Conservation in Northern China", 25 Aug. 2006, (中国側)内蒙古財経学院、内蒙古大学、内蒙古師範大学、内蒙古農業大学 (日本側)茨城大学、拓殖大学
  • マウス皮膚組織におけるカゼイン様タンパク質の発現               
    第; 著者
    日本動物学会関東支部題58回大会, 19 Mar. 2006, 日本動物学会関東支部
  • ヤギ精原細胞の免疫組織学的性状解析               
    第; 著者
    日本動物学会第76回大会予稿集, 06 Oct. 2005
  • ラット脂肪細胞分化に及ぼす共培養成熟脂肪細胞の影響               
    第; 著者
    日本畜産学会第98回大会, 2001
  • ヒツジ乳腺筋上皮細胞の増殖に及ぼす細胞成長因子および高級不飽和脂肪酸の影響               
    第; 著者
    日本畜産学会第97回大会講演要旨集, Mar. 2000
  • C2C12マウス骨格筋由来株化筋芽細胞の増殖に及ぼすビタミンC、ビタミンEおよびセレンの影響               
    第; 著者
    平成9年関東畜産学会講演要旨集, Sep. 1997
  • 初代ウシ骨格筋細胞の増殖に及ぼすビタミンCおよびビタミンEの効果               
    Takuya Kanazawa; Motoaki Kosugiyama
    日本畜産学会第92回大会講演要旨集, 1997
  • ミトコンドリアDNAシトクロムb遺伝子の解析によるヤギおよびヒツジの野生種と家畜種の系統類縁関係の解明               
    第; 著者
    平成7年日本畜産学会関東支部会講演要旨集, 1995
  • 培養マウス乳腺上皮細胞によるカゼイン合成のホルモン支配               
    第; 著者
    第78回日本畜産学会講演要旨集, 1986

Courses

  • Biology II               
    Ibaraki University, School of Agriculture
  • Biology II               
    Ibaraki University, School of Agriculture
  • 茨城大学農学部
  • 茨城大学
  • 茨城大学
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学系研究会
  • 茨城大学農学系研究会
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学系研究科
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学部
  • 茨城大学農学部

Affiliated academic society

  • 2002, Society for the Study of Reproduction
  • Sep. 1995, 日本動物学会
  • 1986, Societe Franco-Japonaise de Biologie
  • Oct. 1985, Society for Biology of Mammary Glands and Lactation

Social Contribution Activities

  • President of the Society of Biology for Mammary Gland and Lactation
    editor
    Office of the Society of Biology for Mammary Gland and Lactation, Workshop of Biology of Mammary Gland and Lactation, 30 Nov. 2013 - Present
  • 50Th Memorial Meeting of the Society of Mammary Gland Biology and Lactation              
    presenter
    07 Dec. 2019
  • 49Th Annual Meeting of the Society of Mammary Gland Biology and Lactation              
    presenter
    01 Dec. 2018
  • 48Th Annual meeting of the Society of Mammary Gland Biology and Lactation              
    presenter
    02 Dec. 2017
  • 48Th Annual Meeting of the Society of Mammary Gland Biology and Lactation              
    presenter
    02 Dec. 2017
  • 47Th Annual Meeting of the Society of Mammary Gland Biology and Lactation              
    presenter
    乳腺・泌乳研究会, 第47回乳腺・泌乳研究会研究集会, 05 Nov. 2016 - 05 Nov. 2016
  • 第46回乳腺・泌乳研究会研究集会の主催および座長              
    presenter
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 05 Dec. 2015 - 05 Dec. 2015
  • 第45回乳腺・泌乳研究会研究集会の主催および座長              
    presenter
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 08 Nov. 2014 - 08 Nov. 2014
  • 第44回乳腺・泌乳研究会研究集会の主催および座長              
    presenter
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 30 Nov. 2013 - 30 Nov. 2013

Media Coverage

  • 47Th Annual Meeting of the Society of Mammary Gland Biology and Lactation           
    乳腺・泌乳研究会, 第47回乳腺・泌乳研究会研究集会, 05 Nov. 2016, Others
  • 第47回乳腺・泌乳研究会研究集会 座長           
    乳腺・泌乳研究会, 第47回乳腺・泌乳研究会研究集会, 05 Nov. 2016, Others
  • 第46回乳腺・泌乳研究会研究集会の主催および座長           
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 05 Dec. 2015, Others
  • 第46回乳腺・泌乳研究会研究集会 座長           
    乳腺・泌乳研究会, 第46回乳腺・泌乳研究会研究集会, 05 Dec. 2015, Others
  • 第45回乳腺・泌乳研究会研究集会の主催および座長           
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 08 Nov. 2014, Others
  • 第45回乳腺・泌乳研究会研究集会 座長           
    乳腺・泌乳研究会, 第45回乳腺・泌乳研究会研究集会, 08 Nov. 2014, Others
  • 第44回乳腺・泌乳研究会研究集会の主催および座長           
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 30 Nov. 2013, Others
  • 第44回乳腺・泌乳研究会 座長           
    乳腺・泌乳研究会, 第44回乳腺・泌乳研究会, 30 Nov. 2013, Others

Academic Contribution Activities

  • 〔Major achievements〕第51回乳腺・泌乳研究会研究集会               
    Planning etc
    金澤卓弥(研究会代表), 茨城大学阿見キャンパス, 23 Mar. 2024 - 23 Mar. 2024